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214 results on '"Calero-Bernal, R."'

1. Genotyping of European Toxoplasma gondii strains by a new high-resolution next-generation sequencing-based method

Author

Joeres, M., Maksimov, P., Höper, D., Calvelage, S., Calero-Bernal, R., Fernández-Escobar, M., Koudela, B., Blaga, R., Vrhovec, M. Globokar, Stollberg, K., Bier, N., Sotiraki, S., Sroka, J., Piotrowska, W., Kodym, P., Basso, W., Conraths, F. J., Mercier, A., Galal, L., Dardé, M. L., Balea, A., Spano, F., Schulze, C., Peters, M., Scuda, N., Lundén, A., Davidson, R. K., Terland, R., Waap, H., de Bruin, E., Vatta, P., Caccio, S., Ortega-Mora, L. M., Jokelainen, P., and Schares, G.

Published
2023
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3. Genotyping of European Toxoplasma gondii strains by a new high-resolution next-generation sequencing-based method

Author

VPDC pathologie, Dutch Wildlife Health Centre (DWHC), Joeres, M., Maksimov, P., Höper, D., Calvelage, S., Calero-Bernal, R., Fernández-Escobar, M., Koudela, B., Blaga, R., Vrhovec, M. Globokar, Stollberg, K., Bier, N., Sotiraki, S., Sroka, J., Piotrowska, W., Kodym, P., Basso, W., Conraths, F. J., Mercier, A., Galal, L., Dardé, M. L., Balea, A., Spano, F., Schulze, C., Peters, M., Scuda, N., Lundén, A., Davidson, R. K., Terland, R., Waap, H., de Bruin, E., Vatta, P., Caccio, S., Ortega-Mora, L. M., Jokelainen, P., Schares, G., VPDC pathologie, Dutch Wildlife Health Centre (DWHC), Joeres, M., Maksimov, P., Höper, D., Calvelage, S., Calero-Bernal, R., Fernández-Escobar, M., Koudela, B., Blaga, R., Vrhovec, M. Globokar, Stollberg, K., Bier, N., Sotiraki, S., Sroka, J., Piotrowska, W., Kodym, P., Basso, W., Conraths, F. J., Mercier, A., Galal, L., Dardé, M. L., Balea, A., Spano, F., Schulze, C., Peters, M., Scuda, N., Lundén, A., Davidson, R. K., Terland, R., Waap, H., de Bruin, E., Vatta, P., Caccio, S., Ortega-Mora, L. M., Jokelainen, P., and Schares, G.

Published
2024

5. Genotyping of European Toxoplasma gondii strains by a new high-resolution next-generation sequencing-based method.

Author

Joeres, M., Maksimov, P., Höper, D., Calvelage, S., Calero-Bernal, R., Fernández-Escobar, M., Koudela, B., Blaga, R., Vrhovec, M. Globokar, Stollberg, K., Bier, N., Sotiraki, S., Sroka, J., Piotrowska, W., Kodym, P., Basso, W., Conraths, F. J., Mercier, A., Galal, L., and Dardé, M. L.

Subjects
TOXOPLASMA gondii, SINGLE nucleotide polymorphisms, WHOLE genome sequencing, NUCLEOTIDE sequencing, HAPLOTYPES, GENOTYPES
Abstract

Purpose: A new high-resolution next-generation sequencing (NGS)-based method was established to type closely related European type II Toxoplasma gondii strains. Methods: T. gondii field isolates were collected from different parts of Europe and assessed by whole genome sequencing (WGS). In comparison to ME49 (a type II reference strain), highly polymorphic regions (HPRs) were identified, showing a considerable number of single nucleotide polymorphisms (SNPs). After confirmation by Sanger sequencing, 18 HPRs were used to design a primer panel for multiplex PCR to establish a multilocus Ion AmpliSeq typing method. Toxoplasma gondii isolates and T. gondii present in clinical samples were typed with the new method. The sensitivity of the method was tested with serially diluted reference DNA samples. Results: Among type II specimens, the method could differentiate the same number of haplotypes as the reference standard, microsatellite (MS) typing. Passages of the same isolates and specimens originating from abortion outbreaks were identified as identical. In addition, seven different genotypes, two atypical and two recombinant specimens were clearly distinguished from each other by the method. Furthermore, almost all SNPs detected by the Ion AmpliSeq method corresponded to those expected based on WGS. By testing serially diluted DNA samples, the method exhibited a similar analytical sensitivity as MS typing. Conclusion: The new method can distinguish different T. gondii genotypes and detect intra-genotype variability among European type II T. gondii strains. Furthermore, with WGS data additional target regions can be added to the method to potentially increase typing resolution. [ABSTRACT FROM AUTHOR]

Published
2024
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8. Experimental infection of sheep at mid-pregnancy with archetypal type II and type III Toxoplasma gondii isolates exhibited different phenotypic traits

Author

Junta de Castilla y León, European Commission, Ministerio de Economía, Industria y Competitividad (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia e Innovación (España), Benavides, Julio [0000-0001-9706-100X], Arteche Villasol, Noive [0000-0001-5793-9578], Ferreras Estrada, Mª del Carmen [0000-0003-1996-2229], Pérez Pérez, Valentín [0000-0003-0075-1587], Gutiérrez Expósito, Daniel [0000-0002-7683-623X], Vallejo, R., Benavides, Julio, Arteche-Villasol, Noive, Sánchez-Sánchez, R., Calero-Bernal, R., Ferreras, Mª del Carmen, Criado, M., Pérez Pérez, Valentín, Ortega-Mora, L.M., Gutiérrez-Expósito, Daniel, Junta de Castilla y León, European Commission, Ministerio de Economía, Industria y Competitividad (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia e Innovación (España), Benavides, Julio [0000-0001-9706-100X], Arteche Villasol, Noive [0000-0001-5793-9578], Ferreras Estrada, Mª del Carmen [0000-0003-1996-2229], Pérez Pérez, Valentín [0000-0003-0075-1587], Gutiérrez Expósito, Daniel [0000-0002-7683-623X], Vallejo, R., Benavides, Julio, Arteche-Villasol, Noive, Sánchez-Sánchez, R., Calero-Bernal, R., Ferreras, Mª del Carmen, Criado, M., Pérez Pérez, Valentín, Ortega-Mora, L.M., and Gutiérrez-Expósito, Daniel

Abstract

Toxoplasma gondii is a major cause of reproductive failure in small ruminants. Genotypic diversity of T. gondii strains has been associated with variations in phenotypic traits in in vitro and murine models. However, whether such diversity could influence the outcome of infection in small ruminants remains mostly unexplored. Here, we investigate the outcome of oral challenge in sheep at mid-pregnancy with 10 sporulated oocysts from three different T. gondii isolates belonging to archetypal II and III and selected according to their genetic and phenotypic variations shown in previous studies. Seventy-three pregnant sheep were divided in four groups: G1 infected with TgShSp1 isolate (type II, ToxoDB#3), G2 with TgShSp16 isolate (type II, ToxoDB#3), G3 with TgShSp24 isolate (type III, ToxoDB#2) and G4 of uninfected control sheep. Two different approaches were carried out within this study: (i) the outcome for the pregnancy after infection (n = 33) and (ii) the lesions and parasite tropism and burden at 14 and 28 days post infection (dpi) (n = 40). The onset of hyperthermia and seroconversion occurred one and two days later, respectively in G1 when compared to G2 and G3. However, sheep that suffered from reproductive failure, either by abortion, foetal dead at the time of euthanasia or stillbirth were similar among infected groups (50%, 40% and 47%, respectively). Histological lesions in placentomes and foetal tissues from euthanized animals from the second approach were only detected at 28 dpi and mainly in G1. At 14 dpi, T. gondii-DNA was only detected in G1 in the 11% of the placentomes. However, at 28 dpi the frequency of detection in placentomes was higher in G1 (96%) than in G2 and G3 (7% and 47%, respectively) besides in foetuses was lower in G2 (20%) than in G1 and G3 (100% and 87%, respectively). Regarding late abortions, stillbirths, and lambs of G1, G2 and G3, the frequency of microscopic lesions was similar between groups (79%, 78% and 67%, respectively) whe

Published
2023

9. Presencia y potencial zoonósico de Cryptosporidium spp., Giardia duodenalis y Balantioides coli en ungulados en libertad y semilibertad en España

Author

Balseiro Morales, Ana María [0000-0002-5121-7264], Dashti, A., Köster, P.C., Bailo, B., Sánchez de las Matas, A., Habel, M.A., Rivero Juarez, A., Vicente, J., Serrano, E., Arnal, M.C., Fernández de Luco, D., Morrondo, P., Armenteros, J.A., Balseiro, Ana, Cardona, G.A., Martínez Carrasco, C., Ortiz, J.A., Carpio, A.J., Calero Bernal, R., González-Barrio, D., Carmena, D., Balseiro Morales, Ana María [0000-0002-5121-7264], Dashti, A., Köster, P.C., Bailo, B., Sánchez de las Matas, A., Habel, M.A., Rivero Juarez, A., Vicente, J., Serrano, E., Arnal, M.C., Fernández de Luco, D., Morrondo, P., Armenteros, J.A., Balseiro, Ana, Cardona, G.A., Martínez Carrasco, C., Ortiz, J.A., Carpio, A.J., Calero Bernal, R., González-Barrio, D., and Carmena, D.

Published
2023

10. Experimental infection of sheep at mid-pregnancy with archetypal type II and type III Toxoplasma gondii isolates exhibited different phenotypic traits

Author

Benavides, Julio [0000-0001-9706-100X], Arteche Villasol, Noive [0000-0001-5793-9578], Ferreras Estrada, Mª del Carmen [0000-0003-1996-2229], Pérez Pérez, Valentín [0000-0003-0075-1587], Gutiérrez Expósito, Daniel [0000-0002-7683-623X], Vallejo García, Raquel, Benavides, Julio, Arteche-Villasol, Noive, Sánchez-Sánchez, R., Calero-Bernal, R., Ferreras, Mª del Carmen, Pérez, Valentín, Ortega-Mora, L.M., Gutiérrez-Expósito, Daniel, Benavides, Julio [0000-0001-9706-100X], Arteche Villasol, Noive [0000-0001-5793-9578], Ferreras Estrada, Mª del Carmen [0000-0003-1996-2229], Pérez Pérez, Valentín [0000-0003-0075-1587], Gutiérrez Expósito, Daniel [0000-0002-7683-623X], Vallejo García, Raquel, Benavides, Julio, Arteche-Villasol, Noive, Sánchez-Sánchez, R., Calero-Bernal, R., Ferreras, Mª del Carmen, Pérez, Valentín, Ortega-Mora, L.M., and Gutiérrez-Expósito, Daniel

Published
2023

19. Neosporosis in Pigs

Author

Dubey, J. P., primary, Hemphill, A., additional, Calero-Bernal, R., additional, and Schares, G., additional

Published
2017
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22. General Biology

Author

Dubey, J. P., primary, Hemphill, A., additional, Calero-Bernal, R., additional, and Schares, G., additional

Published
2017
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24. Techniques

Author

Dubey, J. P., primary, Hemphill, A., additional, Calero-Bernal, R., additional, and Schares, G., additional

Published
2017
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35. Neosporosis in Dogs

Author

Dubey, J. P., primary, Hemphill, A., additional, Calero-Bernal, R., additional, and Schares, G., additional

Published
2017
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36. Toxoplasmosis ovina: falta de correlación entre la gravedad de las lesiones o la respuesta inmunitaria local y las consecuencias clínicas de la infección con tres aislados diferentes de T. gondii

Author

Benavides, Julio [0000-0001-9706-100X], Arteche Villasol, Noive [0000-0001-5793-9578], Ferreras Estrada, Mª del Carmen [0000-0003-1996-2229], Pérez Pérez, Valentín [0000-0003-0075-1587], Gutiérrez Expósito, Daniel [0000-0002-7683-623X], Vallejo, R., Benavides, Julio, Arteche-Villasol, Noive, Sánchez-Sánchez, R., Calero-Bernal, R., Ferreras, Mª del Carmen, Pérez Pérez, Valentín, Ortega Mora, Luis M., Gutiérrez-Expósito, Daniel, Benavides, Julio [0000-0001-9706-100X], Arteche Villasol, Noive [0000-0001-5793-9578], Ferreras Estrada, Mª del Carmen [0000-0003-1996-2229], Pérez Pérez, Valentín [0000-0003-0075-1587], Gutiérrez Expósito, Daniel [0000-0002-7683-623X], Vallejo, R., Benavides, Julio, Arteche-Villasol, Noive, Sánchez-Sánchez, R., Calero-Bernal, R., Ferreras, Mª del Carmen, Pérez Pérez, Valentín, Ortega Mora, Luis M., and Gutiérrez-Expósito, Daniel

Published
2022

37. Response to Toxoplasma gondii in vitro infection of macrophages and neutrophils from vaccinated sheep

Author

Ministerio de Economía y Competitividad (España), Gutiérrez Expósito, Daniel [0000-0002-7683-623X], Arteche Villasol, Noive [0000-0001-5793-9578], Ferreras Estrada, Mª del Carmen [0000-0003-1996-2229], Pérez Pérez, Valentín [0000-0003-0075-1587], Benavides, Julio [0000-0001-9706-100X], Gutiérrez-Expósito, Daniel, Arteche-Villasol, Noive, Vallejo, R., Calero-Bernal, R., Ferreras, Mª del Carmen, Pérez Pérez, Valentín, Ortega-Mora, L.M., Benavides, Julio, Ministerio de Economía y Competitividad (España), Gutiérrez Expósito, Daniel [0000-0002-7683-623X], Arteche Villasol, Noive [0000-0001-5793-9578], Ferreras Estrada, Mª del Carmen [0000-0003-1996-2229], Pérez Pérez, Valentín [0000-0003-0075-1587], Benavides, Julio [0000-0001-9706-100X], Gutiérrez-Expósito, Daniel, Arteche-Villasol, Noive, Vallejo, R., Calero-Bernal, R., Ferreras, Mª del Carmen, Pérez Pérez, Valentín, Ortega-Mora, L.M., and Benavides, Julio

Published
2022

38. Infection outcomes after challenge of sheep at mid-pregnancy with Toxoplasma gondii isolates showing different phenotypic traits

Author

Benavides, Julio [0000-0001-9706-100X], Arteche Villasol, Noive [0000-0001-5793-9578], Ferreras Estrada, Mª del Carmen [0000-0003-1996-2229], Pérez Pérez, Valentín [0000-0003-0075-1587], Gutiérrez Expósito, Daniel [0000-0002-7683-623X], Vallejo, R., Benavides, Julio, Arteche-Villasol, Noive, Sánchez-Sanchez, R., Calero-Bernal, R., Ferreras, Mª del Carmen, Pérez Pérez, Valentín, Ortega-Mora, L.M., Gutiérrez-Expósito, Daniel, Benavides, Julio [0000-0001-9706-100X], Arteche Villasol, Noive [0000-0001-5793-9578], Ferreras Estrada, Mª del Carmen [0000-0003-1996-2229], Pérez Pérez, Valentín [0000-0003-0075-1587], Gutiérrez Expósito, Daniel [0000-0002-7683-623X], Vallejo, R., Benavides, Julio, Arteche-Villasol, Noive, Sánchez-Sanchez, R., Calero-Bernal, R., Ferreras, Mª del Carmen, Pérez Pérez, Valentín, Ortega-Mora, L.M., and Gutiérrez-Expósito, Daniel

Published
2022

39. Toxoplasmosis ovina: falta de correlación entre la gravedad de las lesiones o la respuesta inmunitaria local y las consecuencias clínicas de la infección con tres aislados diferentes de T. gondii

Author

Vallejo, R., Benavides, Julio, Arteche, N., Sánchez-Sánchez, R., Calero-Bernal, R., Ferreras, Mª del Carmen, Pérez Pérez, Valentín, Ortega Mora, Luis M., Gutiérrez-Expósito, D., Benavides, Julio [0000-0001-9706-100X], Arteche Villasol, Noive [0000-0001-5793-9578], Ferreras Estrada, Mª del Carmen [0000-0003-1996-2229], Pérez Pérez, Valentín [0000-0003-0075-1587], Gutiérrez Expósito, Daniel [0000-0002-7683-623X], Benavides, Julio, Arteche Villasol, Noive, Ferreras Estrada, Mª del Carmen, Pérez Pérez, Valentín, and Gutiérrez Expósito, Daniel

Abstract

Trabajo presentado a la: XXXIII Reunión de la Sociedad Española de Anatomía Patológica Veterinaria (SEAPV). Lugo. O19. 15-17 junio.

Published
2022

40. Response to Toxoplasma gondii in vitro infection of macrophages and neutrophils from vaccinated sheep

Author

Gutiérrez-Expósito, D., Arteche, Noive, Vallejo, R., Calero-Bernal, R., Ferreras, Mª del Carmen, Pérez Pérez, Valentín, Ortega-Mora, L. M., Benavides Silván, Julio, Ministerio de Economía y Competitividad (España), Gutiérrez Expósito, Daniel, Arteche Villasol, Noive, Ferreras Estrada, Mª del Carmen, Pérez Pérez, Valentín, and Benavides, Julio

Abstract

Trabajo presentado al: 6th International Meeting on Apicomplexan Parasites in Farm Animals. P-33. Berna (Suiza), 5-7 de octubre., This work was supported by the Spanish Ministry of Economy and Competitiveness (PID2019- 104713RB-C22).

Published
2022

41. Infection outcomes after challenge of sheep at mid-pregnancy with Toxoplasma gondii isolates showing different phenotypic traits

Author

Vallejo, R., Benavides Silván, Julio, Arteche, Noive, Sánchez-Sanchez, R., Calero-Bernal, R., Ferreras, Mª del Carmen, Pérez Pérez, Valentín, Ortega-Mora, L. M., Gutiérrez-Expósito, D., Benavides, Julio [0000-0001-9706-100X], Arteche Villasol, Noive [0000-0001-5793-9578], Ferreras Estrada, Mª del Carmen [0000-0003-1996-2229], Pérez Pérez, Valentín [0000-0003-0075-1587], Gutiérrez Expósito, Daniel [0000-0002-7683-623X], Benavides, Julio, Arteche Villasol, Noive, Ferreras Estrada, Mª del Carmen, Pérez Pérez, Valentín, and Gutiérrez Expósito, Daniel

Abstract

Trabajo presentado al: 6th International Meeting on Apicomplexan Parasites in Farm Animals. O-30. Berna (Suiza), 5-7 de octubre.

Published
2022

42. Influencia de la variabilidad en la virulencia de diferentes aislados de Toxoplasma gondii sobre las lesiones de encéfalos fetales ovinos

Author

Vallejo, R., Seehusen, F., Kipar, A., Arteche, N., Sánchez Sánchez, R., Calero Bernal, R., Ferreras, Mª del Carmen, Ortega Mora, Luis M., Gutiérrez-Expósito, D., Pérez Pérez, Valentín, Benavides, Julio, Arteche Villasol, Noive, Ferreras Estrada, Mª del Carmen, Gutiérrez Expósito, Daniel, Pérez Pérez, Valentín, Benavides, Julio, Arteche Villasol, Noive [0000-0001-5793-9578], Ferreras Estrada, Mª del Carmen [0000-0003-1996-2229], Gutiérrez Expósito, Daniel [0000-0002-7683-623X], Pérez Pérez, Valentín [0000-0003-0075-1587], and Benavides, Julio [0000-0001-9706-100X]

Abstract

Trabajo presentado a la: XXXII Reunión de la Sociedad Española de Anatomía Patológica Veterinaria (SEAPV). 1 octubre. Congreso virtual.

Published
2021

43. Cytokine and TLR profiles of monocyte-derived macrophages from vaccinated and non-vaccinated ewes after in vitro infection with toxoplasma gondii

Author

Gutiérrez-Expósito, D., Arteche, N., Vallejo, R., Calero Bernal, R., Ortega Mora, Luis M., Ferreras, Mª del Carmen, Pérez Pérez, Valentín, Benavides, Julio, Gutiérrez Expósito, Daniel, Arteche Villasol, Noive, Ferreras Estrada, Mª del Carmen, Pérez Pérez, Valentín, Benavides, Julio, Gutiérrez Expósito, Daniel [0000-0002-7683-623X], Arteche Villasol, Noive [0000-0001-5793-9578], Ferreras Estrada, Mª del Carmen [0000-0003-1996-2229], Pérez Pérez, Valentín [0000-0003-0075-1587], and Benavides, Julio [0000-0001-9706-100X]

Subjects
Sheep, Vaccination, Immune response, Toxoplasmosis
Abstract

Trabajo presentado al: 7th European Veterinary Immunology Workshop. Livestreaming. 29-31 agosto.

Published
2021

48. Molecular survey of Besnoitia spp. (Apicomplexa) in faeces from European wild mesocarnivores in Spain. Short running title: Molecular survey of Besnoitia spp in faeces from wild carnivores

Author

Martín-Pérez M, Marta Mateo, Miguel A. Habela, Ana Balseiro, Alvarez-Garcia G, María Jesús Palacios, González-Barrio D, David Carmena, Fernández-García J, Fernando Nájera, Calero-Bernal R, Barrel M, Pamela C. Köster, and Figueiredo A

Subjects
Apicomplexa, biology, Zoology, Besnoitia, biology.organism_classification, Feces
Abstract

Numerous studies have unsuccessfully tried to unravel the definitive host of the coccidian parasite Besnoitia besnoiti. Cattle infections by B. besnoiti cause a chronic and debilitating condition called bovine besnoitiosis that has emerged in Europe during the last two decades, mainly due to limitations in its control associated to the absence of vaccines and therapeutical tools. Although the exact transmission pathway of B. besnoiti is currently unknown, it is assumed that the parasite might have an indirect life cycle with a carnivore as definitive host. Current lack of studies in wildlife might underestimate the importance of free-living species in the epidemiology of B. besnoiti. Thus, the aim of the present study is to assess the presence of Besnoitia spp. in free-ranging mesocarnivores in Spain. DNA was searched by PCR on faeces collected from wild carnivores as a first approach to determine which species could be considered as potential definitive host candidates in further research. For this purpose, a total of 352 faecal samples from 12 free-living wild carnivore species belonging to the Canidae, Felidae, Herpestidae, Mustelidae, Procyonidae, and Viverridae families were collected in seven Spanish regions. PCR testing showed that Besnoitia spp. DNA was present in four faecal samples from red foxes collected in western Spain, an area with the greatest density of extensively reared cattle and associated to high incidence of bovine besnoitiosis in the country. To date, this is the first report of a Besnoitia besnoiti-like sequence (99.57% homology) from carnivore faeces in a worldwide context. Red foxes might contribute to the epidemiology of B. besnoiti, although further studies, mostly based on bioassay, would be needed to elucidate the accuracy and extent of these interesting findings.

Published
2021

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