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6. Methodologies for in vitro and in vivo evaluation of efficacy of antifungal and antibiofilm agents and surface coatings against fungal biofilms

Author

Van Dijck, P., Sjollema, J., Cammue, B.P., Lagrou, K., Berman, J., d'Enfert, C., Andes, D.R., Arendrup, M.C., Brakhage, A.A., Calderone, R., Cantón, E., Coenye, T., Cos, P., Cowen, L.E., Edgerton, M., Espinel-Ingroff, A., Filler, S.G., Ghannoum, M., Gow, NAR, Haas, H., Jabra-Rizk, M.A., Johnson, E.M., Lockhart, S.R., Lopez-Ribot, J.L., Maertens, J., Munro, C.A., Nett, J.E., Nobile, C.J., Pfaller, M.A., Ramage, G., Sanglard, D., Sanguinetti, M., Spriet, I., Verweij, P.E., Warris, A., Wauters, J., Yeaman, M.R., Zaat, SAJ, and Thevissen, K.

Subjects
antibiofilm material coating, antifungal susceptibility testing, biofilm eradication, biofilm inhibition, in vivo models
Abstract

Unlike superficial fungal infections of the skin and nails, which are the most common fungal diseases in humans, invasive fungal infections carry high morbidity and mortality, particularly those associated with biofilm formation on indwelling medical devices. Therapeutic management of these complex diseases is often complicated by the rise in resistance to the commonly used antifungal agents. Therefore, the availability of accurate susceptibility testing methods for determining antifungal resistance, as well as discovery of novel antifungal and antibiofilm agents, are key priorities in medical mycology research. To direct advancements in this field, here we present an overview of the methods currently available for determining (i) the susceptibility or resistance of fungal isolates or biofilms to antifungal or antibiofilm compounds and compound combinations; (ii) the in vivo efficacy of antifungal and antibiofilm compounds and compound combinations; and (iii) the in vitro and in vivo performance of anti-infective coatings and materials to prevent fungal biofilm-based infections.

Published
2018

10. In vitro models for Candida biofilm development

Author

Krom, B.P., Willems, H.M.E., Calderone, R., Cihlar, R., Preventieve tandheelkunde (OII, ACTA), Preventive Dentistry, Calderone, R., and Cihlar, R.

Subjects
0301 basic medicine, In Vitro Techniques, 030106 microbiology, Microfluidics, Biofilm, Model system, Biology, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, In vitro, Microbiology, 03 medical and health sciences, Biophysics, Candida spp, Candida albicans
Abstract

Development of Candida spp. biofilms on medical devices such as catheters and voice prosthesis has been recognized as an increasing clinical problem. Different in vitro models are presented with increasing complexity. Each model system can be utilized for analysis of new active compounds to prevent or treat Candida biofilms as well as to study molecular processes involved in biofilm formation. Susceptibility studies of clinical isolates are generally performed in a simple 96-well model system similar to the CLSI standard. In the present chapter, optimized conditions that promote biofilm formation within individual wells of microtiter plates are described. In addition, the method has proven useful in preparing C. albicans biofilms for investigation by a variety of microscopic and molecular techniques. A more realistic and more complex biofilm system is presented by the Amsterdam Active Attachment (AAA) model. In this 24-well model all crucial steps of biofilm formation: adhesion, proliferation, and maturation, can be simulated on various surfaces, while still allowing a medium throughput approach. This model has been applied to study susceptibility, complex molecular mechanisms as well as interspecies (Candida–bacterium) interactions. Finally, a realistic microfluidics channel system is presented to follow dynamic processes in biofilm formation. In this Bioflux-based system, molecular mechanisms as well as dynamic processes can be studied at a high time-resolution.

Published
2016

11. Methodologies for in vitro and in vivo evaluation of efficacy of antifungal and antibiofilm agents and surface coatings against fungal biofilms

Author

Dijck, P. Van, Sjollema, J., Cammue, B.P., Lagrou, K., Berman, J., D'Enfert, C., Andes, D.R., Arendrup, M.C., Brakhage, A.A., Calderone, R., Canton, E., Coenye, T., Cos, P., Cowen, L.E., Edgerton, M., Espinel-Ingroff, A., Filler, S.G., Ghannoum, M., Gow, N.A., Haas, H. de, Jabra-Rizk, M.A., Johnson, Emma C., Lockhart, S.R., Lopez-Ribot, J.L., Maertens, J., Munro, C.A., Nett, J.E., Nobile, C.J., Pfaller, M.A., Ramage, G., Sanglard, D., Sanguinetti, M., Spriet, I., Verweij, P.E., Warris, A., Wauters, J., Yeaman, M.R., Zaat, S.A., Thevissen, K., Dijck, P. Van, Sjollema, J., Cammue, B.P., Lagrou, K., Berman, J., D'Enfert, C., Andes, D.R., Arendrup, M.C., Brakhage, A.A., Calderone, R., Canton, E., Coenye, T., Cos, P., Cowen, L.E., Edgerton, M., Espinel-Ingroff, A., Filler, S.G., Ghannoum, M., Gow, N.A., Haas, H. de, Jabra-Rizk, M.A., Johnson, Emma C., Lockhart, S.R., Lopez-Ribot, J.L., Maertens, J., Munro, C.A., Nett, J.E., Nobile, C.J., Pfaller, M.A., Ramage, G., Sanglard, D., Sanguinetti, M., Spriet, I., Verweij, P.E., Warris, A., Wauters, J., Yeaman, M.R., Zaat, S.A., and Thevissen, K.

Abstract

Contains fulltext : 196638.pdf (publisher's version ) (Open Access), Unlike superficial fungal infections of the skin and nails, which are the most common fungal diseases in humans, invasive fungal infections carry high morbidity and mortality, particularly those associated with biofilm formation on indwelling medical devices. Therapeutic management of these complex diseases is often complicated by the rise in resistance to the commonly used antifungal agents. Therefore, the availability of accurate susceptibility testing methods for determining antifungal resistance, as well as discovery of novel antifungal and antibiofilm agents, are key priorities in medical mycology research. To direct advancements in this field, here we present an overview of the methods currently available for determining (i) the susceptibility or resistance of fungal isolates or biofilms to antifungal or antibiofilm compounds and compound combinations; (ii) the in vivo efficacy of antifungal and antibiofilm compounds and compound combinations; and (iii) the in vitro and in vivo performance of anti-infective coatings and materials to prevent fungal biofilm-based infections.

Published
2018

12. Identification of Secreted Candida Proteins Using Mass Spectrometry

Author

Gómez-Molero, E., Dekker, H.L., de Boer, A.D., de Groot, P.W., Calderone, R., Cihlar, R., and Mass Spectrometry of Biomacromolecules (SILS, FNWI)

Subjects
0301 basic medicine, Signal peptide, 030106 microbiology, Biology, Trypsin, Bioinformatics, Mass spectrometry, Yeast, Cell wall, 03 medical and health sciences, Secretory protein, Biochemistry, Proteome, medicine, Secretion, medicine.drug
Abstract

Analysis of fungal secretomes using mass spectrometry is a useful technique in cell biology. Knowledge of the secretome of a human fungal pathogen may yield important information of host-pathogen interactions and may be useful for identifying vaccines candidates or diagnostic markers for antifungal strategies. In this chapter, with a main focus on sample preparation aspects, we describe the methodology that we apply for gel-independent batch identification and quantification of proteins that are secreted during growth in liquid cultures. Using these techniques with Candida and other yeast species, the majority of the identified proteins are classical secretory proteins and cell wall proteins containing N-terminal signal peptides for secretion, although dependent on sample preparation quality and the mass spectrometric analysis also usually, a number of nonsecretory proteins are identified.

Published
2016

19. Raman and AFM piezoresponse study of dense BaTiO3 nanocrystalline ceramics

Author

Buscaglia, V., Buscaglia, M. T., Viviani, M., Ostapchuk, T., Gregora, I., Petzelt, J., Mitoseriu, L., Nanni, P., Testino, A., Calderone, R., Harnagea, C., Zhao, Zhe, Nygren, M., Buscaglia, V., Buscaglia, M. T., Viviani, M., Ostapchuk, T., Gregora, I., Petzelt, J., Mitoseriu, L., Nanni, P., Testino, A., Calderone, R., Harnagea, C., Zhao, Zhe, and Nygren, M.

Abstract

Dense nanocrystalline BaTiO3 (BT) ceramics with grain sizes (GSs) below 100 nm obtained by spark plasma sintering (SPS), were investigated by micro-Raman spectroscopy in order to obtain information about the changes in the local order induced by size effects. The obtained spectra in the range 80-700 K showed the presence of all the crystalline phases of BaTiO3, even in the finest structure (50 nm grain size ceramic), with particularities attributed to the high density of non-ferroelectric grain boundaries. The AFM piezoresponse study incontestably proved the ferroelectric switching at local scale in nanocrystalline BaTiO3 ceramics at room temperature., QC 20120307

Published
2005
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21. Phase I clinical trial of namitecan (ST1968): Results with D1-3 q3wks schedule.

Author

Fasolo, A., primary, Del Conte, G., additional, Calderone, R. G., additional, Gallerani, E., additional, Hagner, N., additional, Barbieri, P., additional, Luraghi, L., additional, Pace, S., additional, Capocasa, F., additional, Coceani, N., additional, Maccioni, E., additional, Hess, D., additional, Sessa, C., additional, and Gianni, L., additional

Published
2011
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24. Raman and AFM piezoresponse study of dense BaTiO3 nanocrystalline ceramics

Author

Buscaglia, V., primary, Buscaglia, M.T., additional, Viviani, M., additional, Ostapchuk, T., additional, Gregora, I., additional, Petzelt, J., additional, Mitoseriu, L., additional, Nanni, P., additional, Testino, A., additional, Calderone, R., additional, Harnagea, C., additional, Zhao, Z., additional, and Nygren, M., additional

Published
2005
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27. FEMS Yeast Research

Author

KRUPPA, M, primary, GOINS, T, additional, CUTLER, J, additional, LOWMAN, D, additional, WILLIAMS, D, additional, CHAUHAN, N, additional, MENON, V, additional, SINGH, P, additional, LI, D, additional, and CALDERONE, R, additional

Published
2003
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32. Candida albicans : adherence, signaling and virulence

Author

Calderone, R., primary, Suzuki, S., additional, Cannon, R., additional, Cho, T., additional, Boyd, D., additional, Calera, J., additional, Chibana, H., additional, Herman, D., additional, Holmes, A., additional, Jeng, H-W., additional, Kaminishi, H., additional, Matsumoto, T., additional, Mikami, T., additional, O'Sullivan, J. M., additional, Sudoh, M., additional, Suzuki, M., additional, Nakashima, Y., additional, Tanaka, T., additional, Tompkins, G. R., additional, and Watanabe, T., additional

Published
2000
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