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Your search keyword '"Calderon, Angie"' showing total 12 results
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12 results on '"Calderon, Angie"'

2. Substrate specificity of FUT8 and chemoenzymatic synthesis of core-fucosylated asymmetric N -glycans

Author

Calderon, Angie D, Liu, Yunpeng, Li, Xu, Wang, Xuan, Chen, Xi, Li, Lei, and Wang, Peng G

Subjects
Organic Chemistry, Chemical Sciences, Biocatalysis, Fucose, Fucosyltransferases, Humans, Polysaccharides, Substrate Specificity, Medicinal and Biomolecular Chemistry, Medicinal and biomolecular chemistry, Organic chemistry
Abstract

Substrate specificity studies of human FUT8 using 77 structurally-defined N-glycans as acceptors showed a strict requirement towards the α1,3-mannose branch, but a great promiscuity towards the α1,6-mannose branch. Accordingly, a chemoenzymatic strategy was developed for the efficient synthesis of core-fucosylated asymmetric N-glycans.

Published
2016

3. Efficient chemoenzymatic synthesis of an N-glycan isomer library

Author

Li, Lei, Liu, Yunpeng, Ma, Cheng, Qu, Jingyao, Calderon, Angie D, Wu, Baolin, Wei, Na, Wang, Xuan, Guo, Yuxi, Xiao, Zhongying, Song, Jing, Sugiarto, Go, Li, Yanhong, Yu, Hai, Chen, Xi, and Wang, Peng George

Subjects
Chemical Sciences
Abstract

Quantification, characterization and biofunctional studies of N-glycans on proteins remain challenging tasks due to complexity, diversity and low abundance of these glycans. The availability of structurally defined N-glycans (especially isomers) libraries is essential to help on solving these tasks. We reported herein an efficient chemoenzymatic strategy, namely Core Synthesis/Enzymatic Extension (CSEE), for rapid production of diverse N-glycans. Starting with 5 chemically prepared building blocks, 8 N-glycan core structures containing one or two terminal N-acetyl-D-glucosamine (GlcNAc) residue(s) were chemically synthesized via consistent use of oligosaccharyl thioethers as glycosylation donors in the convergent fragment coupling strategy. Each of these core structures was then extended to 5 to 15 N-glycan sequences by enzymatic reactions catalyzed by 4 robust glycosyltransferases. Success in synthesizing N-glycans with Neu5Gc and core-fucosylation further expanded the ability of enzymatic extension. High performance liquid chromatography with an amide column enabled rapid and efficient purification (>98% purity) of N-glycans in milligram scales. A total of 73 N-glycans (63 isomers) were successfully prepared and characterized by MS2 and NMR. The CSEE strategy provides a practical approach for "mass production" of structurally defined N-glycans, which are important standards and probes for Glycoscience.

Published
2015

4. Application of Augmented Reality in Teaching and Learning in Engineering Programs

Author

Carrion Silva, Alexis Javier, Reyes Calderon, Angie Melanie, Giraldo Retuerto, Margarita, and Andrade-Arenas, Laberiano

Subjects
learning, university formation, Computer Networks and Communications, distance education, augmented reality, teaching, Computer Science Applications
Abstract

Augmented reality is one of the new emerging technologies in the world, since its appearance was considered for many years its application in education as a tool in the student's learning process. Taking these aspects into account, the present research article was developed, which is based on the design proposal using augmented reality in the learning process at a university in northern Lima. In which the analysis, architecture and development of a prototype was showed in detail. The result of the article was obtained by means of surveys, in the first survey it was demonstrated that the augmented reality is an unknown technology for several of the students and the feasible use in the distance education as a support in the classes, in the same way the second survey demonstrated a high interest on the part of the students at the moment of using this technology in its learning of university formation.

Published
2022

5. Impact of virtual education in times of pandemic at the University of North Lima

Author

Laberiano Andrade-Arenas, Acosta Espinoza-Max, Ventocilla Gomero-Fanny, Aguila Ruiz-Bengy, Cruz Acuna-Jeferson, and Reyes Calderon-Angie

Subjects
2019-20 coronavirus outbreak, Higher education, Work (electrical), Coronavirus disease 2019 (COVID-19), business.industry, Virtual teaching, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Pandemic, ComputingMilieux_COMPUTERSANDEDUCATION, Sociology, Public relations, business
Abstract

This research work is based on the study of the analysis of the impact of the use of technological tools in university higher education, which we will show in detail based on our university environment that will be investigated in Lima, Peru. In it we will explain the methodology that we will use, in addition to the current problems that we have because of the COVID-19 (global pandemic) that influences in great amount the education that the universities offer at national and global level, that went from being in person to virtual in our country. This implied taking measures in which they are adaptable for both students and teachers, who in both cases do not have, for the most part, knowledge about the use of virtual tools towards learning and teaching necessary to have an optimal achievement. The result of the research work has been obtained based on the analysis of the Malteza cross, a tool that allowed us to analyze the information obtained from CATDWE, with its 4 quadrants North-East, North-West, South-East, South-West, from which we obtained that students and teachers adapted to virtual teaching with the technological resources they had at the time.

Published
2020

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