174 results on '"Caiqin Wang"'
Search Results
2. Research progress on the fanconi anemia signaling pathway in non-obstructive azoospermia
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Haohui Xu, Yixin Zhang, Caiqin Wang, Zhuoyan Fu, Jing Lv, Yufang Yang, Zihan Zhang, Yuanmin Qi, Kai Meng, Jinxiang Yuan, and Xiaomei Wang
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non-obstructive azoospermia ,Fanconi anemia pathway ,Fanconi anemia gene ,interstrand crosslinks ,homologous recombination ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
Non-obstructive azoospermia (NOA) is a disease characterized by spermatogenesis failure and comprises phenotypes such as hypospermatogenesis, mature arrest, and Sertoli cell-only syndrome. Studies have shown that FA cross-linked anemia (FA) pathway is closely related to the occurrence of NOA. There are FA gene mutations in male NOA patients, which cause significant damage to male germ cells. The FA pathway is activated in the presence of DNA interstrand cross-links; the key step in activating this pathway is the mono-ubiquitination of the FANCD2-FANCI complex, and the activation of the FA pathway can repair DNA damage such as DNA double-strand breaks. Therefore, we believe that the FA pathway affects germ cells during DNA damage repair, resulting in minimal or even disappearance of mature sperm in males. This review summarizes the regulatory mechanisms of FA-related genes in male azoospermia, with the aim of providing a theoretical reference for clinical research and exploration of related genes.
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- 2024
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3. Elderly patients with stage II gastric cancer do not benefit from adjuvant chemotherapy
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Jianping Guo, Zhizhong Xiong, Shi Yin, Yue’e Wen, Longyang Jin, Caiqin Wang, Huaxian Chen, Dandong Luo, Zijian Deng, Dayin Huang, Xianzhe Li, Biying Yi, Chaobin Mao, and Lei Lian
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Gastric cancer ,Elderly ,Adjuvant chemotherapy ,Survival ,Surgery ,RD1-811 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background With the aging of the population, the burden of elderly gastric cancer (EGC) increases worldwide. However, there is no consensus on the definition of EGC and the efficacy of adjuvant chemotherapy in patients with stage II EGC. Here, we investigated the effectiveness of adjuvant chemotherapy in defined EGC patients. Methods We enrolled 5762 gastric cancer patients of three independent cohorts from the Sixth Affiliated Hospital of Sun Yat-sen University (local), the Surveillance, Epidemiology, and End Results (SEER), and the Asian Cancer Research Group (ACRG). The optimal age cutoff for EGC was determined using the K-adaptive partitioning algorithm. The defined EGC group and the efficacy of adjuvant chemotherapy for them were confirmed by Cox regression and Kaplan–Meier survival analyses. Furthermore, gene set variation analyses (GSVA) were performed to reveal pathway enrichment between groups. Results The optimal age partition value for EGC patients was 75. In the local, SEER, and ACRG cohorts, the EGC group exhibited significantly worse overall survival and cancer-specific survival than the non-EGC group (P
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- 2023
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4. Bond Behavior of High-Strength Steel Rebar in Ultra-High-Performance Manufactured Sand Concrete: Experiment and Modelling
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Caiqin Wang, Yubo Jiao, Jian Xing, and Yaojia Chen
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UHPC ,manufactured sand ,HRB 600 rebar ,pull-out test ,bond-slip behavior ,Building construction ,TH1-9745 - Abstract
Manufactured sand (MS), due to its wide availability and cost-effectiveness, is used as an alternative aggregate for quartz sand (QS) in ultra-high-performance concrete (UHPC) to prepare ultra-high-performance manufactured sand concrete (UHPMC). This study aims to assess the bond behavior of 600 MPa-grade, high-strength, hot-rolled ribbed bars (HRB 600) in UHPMC. Thirty specimens were designed for the pull-out tests, taking into account several feature parameters, including MS replacement ratio (0%, 50%, 100%), water–binder ratio (0.17, 0.19, 0.21), steel fiber content (0%, 1%, 2%), and anchorage length (2d, 3d, 4d, 5d). The effects of the feature parameters on the failure mode, bond stress–slip curves, bond strength, bond-slip mechanism, and constitutive model were analyzed and illustrated. The results reveal that the pull-out specimen of UHPMC exhibits three distinct failure modes: rebar pull-out failure, UHPMC splitting failure, and splitting-pull-out failure. The bond strength increases from 46.57 MPa to 56.92 MPa when the steel fiber content increases from 0% to 2%. Additionally, a decrease in anchoring length is beneficial for improving the bond strength; as the anchoring length increases from 2d to 5d, the bonding strength decreases by 35.84%. The bond strength increases with an increase in the MS replacement ratio. As for the water–binder ratio, the bond strength presents the highest value when the water–binder ratio is 0.17. In addition, a new bond-slip constitutive model applicable to UHPMC and HRB 600 rebar, considering the MS replacement ratio, the water–binder ratio, etc., is proposed, which presents favorable prediction accuracy.
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- 2024
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5. Effect of Composition Characteristics on Mechanical Properties of UHPMC Based on Response Surface Methodology and Acoustic Emission Monitoring
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Ranran Chen, Yubo Jiao, Mingqi Xiao, Hua Yang, and Caiqin Wang
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UHPC ,manufactured sand ,mechanical and flexural properties ,response surface methodology ,AE monitoring ,Technology ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Microscopy ,QH201-278.5 ,Descriptive and experimental mechanics ,QC120-168.85 - Abstract
Manufactured sand (MS) is a promising alternative aggregate to quartz sand (QS) in ultra-high-performance concrete (UHPC) in the preparation of ultra-high-performance manufactured sand concrete (UHPMC), which possesses the characteristics of high strength, low cost, and environmental friendliness. In this study, the effects of variable compositional characteristics including the water–binder ratio, the stone powder (SP) content, and the MS replacement ratio on the mechanical and flexural strength of UHPMC were compared and analyzed based on response surface methodology (RSM). Meanwhile, the damage characteristics of UHPMC during compressive and flexural stress were monitored and evaluated using acoustic emission (AE) technology. The results reveal that the compressive and flexural strengths of UHPMC are both negatively correlated with the water–binder ratio, while they are positively correlated with the MS replacement rate. They tend to firstly increase and subsequently decrease with the increase in the stone powder content. In the load–displacement curve of concrete with a high MS replacement ratio and a low water–binder ratio, the slope in the elastic stage is steeper, the stiffness is higher, and the bending toughness and ductility are also better. The specimens with a 10% to 0% stone powder content present a steeper elastic phase slope, a slightly higher stiffness, and superior ductility. The specimens with a low MS replacement ratio and a high water–binder ratio display earlier cracking and weaker resistance, and the destruction process is complex and very unstable. The damage mode analysis based on RA-AF shows that an increase in the MS replacement ratio and a decrease in the water–binder ratio can both reduce the tensile cracking of UHPMC specimens under a four-point bending test. Although 10% stone powder can marginally slow down crack growth, the failure mode is not significantly affected.
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- 2024
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6. Induction of filopodia formation by α-Actinin-2 via RelA with a feedforward activation loop promoting overt bone marrow metastasis of gastric cancer
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Caiqin Wang, Bo Xie, Shi Yin, Jianghua Cao, Junhao Huang, Longyang Jin, Ge Du, Xiaohui Zhai, Rongqin Zhang, Shanshan Li, Taiyuan Cao, Hongen Yu, Xinjuan Fan, Zuli Yang, Junsheng Peng, Jian Xiao, and Lei Lian
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Gastric cancer ,Bone marrow metastasis ,α-Actinin-2 ,Filopodia formation ,RelA ,Medicine - Abstract
Abstract Background Bone marrow metastasis (BMM) is underestimated in gastric cancer (GC). GC with BMM frequently complicate critical hematological abnormalities like diffused intravascular coagulation and microangiopathic hemolytic anemia, which constitute a highly aggressive GC (HAGC) subtype. HAGC present a very poor prognosis with peculiar clinical and pathological features when compared with not otherwise specified advanced GC (NAGC). But the molecular mechanisms underlying BMM from GC remain rudimentary. Methods The transcriptomic difference between HAGC and NAGC were analyzed. Genes that were specifically upregulated in HAGC were identified, and their effect on cell migration and invasion was studied. The function of ACTN2 gene were confirmed by GC cell lines, bone-metastatic animal model and patients’ tissues. Furthermore, the molecular mechanism of ACTN2 derived-BMM was explored by multiple immunofluorescence staining, western blot, chromatin immunoprecipitation, and luciferase reporter assays. Results We elucidated the key mechanisms of BMM depending on the transcriptomic difference between HAGC and NAGC. Five genes specifically upregulated in HAGC were assessed their effect on cell migration and invasion. The ACTN2 gene encoding protein α-Actinin-2 was detected enhanced the metastatic capability and induced BMM of GC cells in mouse models. Mechanically, α-Actinin-2 was involved in filopodia formation where it promoted the Actin filament cross-linking by replacing α-Actinin-1 to form α-Actinin-2:α-Actinin-4 complexes in GC cells. Moreover, NF-κB subunit RelA and α-Actinin-2 formed heterotrimers in the nuclei of GC cells. As a direct target of RelA:α-Actinin-2 heterotrimers, the ACTN2 gene was a positive auto-regulatory loop for α-Actinin-2 expression. Conclusions We demonstrated a link between filopodia, BMM and ACTN2 activation, where a feedforward activation loop between ACTN2 and RelA is established via actin in response to distant metastasis. Given the novel filopodia formation function and the new mechanism of BMM in GC, we propose ACTN2 as a druggable molecular vulnerability that may provide potential therapeutic benefit against BMM of GC.
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- 2023
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7. Different outer membrane c‐type cytochromes are involved in direct interspecies electron transfer to Geobacter or Methanosarcina species
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Dawn E. Holmes, Jinjie Zhou, Jessica A. Smith, Caiqin Wang, Xinying Liu, and Derek R. Lovley
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c‐type cytochrome ,direct interspecies electron transfer (DIET) ,extracellular electron transfer ,Geobacter ,Methanosarcina ,Microbiology ,QR1-502 - Abstract
Abstract Direct interspecies electron transfer (DIET) may be most important in methanogenic environments, but mechanistic studies of DIET to date have primarily focused on cocultures in which fumarate was the terminal electron acceptor. To better understand DIET with methanogens, the transcriptome of Geobacter metallireducens during DIET‐based growth with G. sulfurreducens reducing fumarate was compared with G. metallireducens grown in coculture with diverse Methanosarcina. The transcriptome of G. metallireducens cocultured with G. sulfurreducens was significantly different from those with Methanosarcina. Furthermore, the transcriptome of G. metallireducens grown with Methanosarcina barkeri, which lacks outer‐surface c‐type cytochromes, differed from those of G. metallireducens cocultured with M. acetivorans or M. subterranea, which have an outer‐surface c‐type cytochrome that serves as an electrical connect for DIET. Differences in G. metallireducens expression patterns for genes involved in extracellular electron transfer were particularly notable. Cocultures with c‐type cytochrome deletion mutant strains, ∆Gmet_0930, ∆Gmet_0557 and ∆Gmet_2896, never became established with G. sulfurreducens but adapted to grow with all three Methanosarcina. Two porin–cytochrome complexes, PccF and PccG, were important for DIET; however, PccG was more important for growth with Methanosarcina. Unlike cocultures with G. sulfurreducens and M. acetivorans, electrically conductive pili were not needed for growth with M. barkeri. Shewanella oneidensis, another electroactive microbe with abundant outer‐surface c‐type cytochromes, did not grow via DIET. The results demonstrate that the presence of outer‐surface c‐type cytochromes does not necessarily confer the capacity for DIET and emphasize the impact of the electron‐accepting partner on the physiology of the electron‐donating DIET partner.
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- 2022
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8. Luteolin regulates the distribution and function of organelles by controlling SIRT1 activity during postovulatory oocyte aging
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Xupeng Xing, Jingfeng Peng, Jingyu Zhao, Ruoxi Shi, Caiqin Wang, Zihan Zhang, Zihan Wang, Zicong Li, and Zhenfang Wu
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luteolin ,postovulatory oocyte aging ,aging-induced changes ,organelles ,SIRT1 ,Nutrition. Foods and food supply ,TX341-641 - Abstract
The quality of oocytes determines their development competence, which will be rapidly lost if the oocytes are not fertilized at the proper time after ovulation. SIRT1, one of the sirtuin family members, has been proven to protect the quality of oocytes during postovulatory oocyte aging. However, evidence of the effect of SIRT1 on the activity of organelles including the mitochondria, the endoplasmic reticulum (ER), the Golgi apparatus, and the lysosomes in postovulatory aging oocyte is lacking. In this study, we investigated the distribution and function of organelles in postovulatory aged oocytes and discovered abnormalities. Luteolin, which is a natural flavonoid contained in vegetables and fruits, is an activator of SIRT1. When the oocytes were treated with luteolin, the abnormal distribution of mitochondria, ER, and Golgi complex were restored during postovulatory oocyte aging. The ER stress protein GRP78 and the lysosome protein LAMP1 increased, while the mitochondrial membrane potential and the Golgi complex protein GOLPH3 decreased in aged oocytes, and these were restored by luteolin treatment. EX-527, an inhibitor of SIRT1, disrupted the luteolin-mediated normal distribution and function of mitochondria, ER, Golgi apparatus, and lysosomes. In conclusion, we demonstrate that luteolin regulates the distribution and function of mitochondria, ER, Golgi apparatus, and lysosomes during postovulatory oocyte aging by activating SIRT1.
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- 2023
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9. Bone marrow metastasis from advanced gastric cancer complicated with disseminated intravascular coagulation: a highly aggressive but manageable disease subtype
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Xiaohui Zhai, Caiqin Wang, Shanshan Li, Taiyuan Cao, Ge Du, Yan Zhang, Dianke Chen, Xinjuan Fan, Yan Huang, Ping Hu, Jian Zheng, Zuli Yang, Lei Lian, Junsheng Peng, Ping Lan, and Jian Xiao
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Published
- 2022
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10. Correction to: ALG3 contributes to stemness and radioresistance through regulating glycosylation of TGF-β receptor II in breast cancer
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Xiaoqing Sun, Zhenyu He, Ling Guo, Caiqin Wang, Chuyong Lin, Liping Ye, Xiaoqing Wang, Yue Li, Meisongzhu Yang, Sailan Liu, Xin Hua, Wen Wen, Chao Lin, Zhiqing Long, Wenwen Zhang, Han Li, Yunting Jian, Ziyuan Zhu, Xianqiu Wu, and Huanxin Lin
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Published
- 2022
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11. ALG3 contributes to stemness and radioresistance through regulating glycosylation of TGF-β receptor II in breast cancer
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Xiaoqing Sun, Zhenyu He, Ling Guo, Caiqin Wang, Chuyong Lin, Liping Ye, Xiaoqing Wang, Yue Li, Meisongzhu Yang, Sailan Liu, Xin Hua, Wen Wen, Chao Lin, Zhiqing Long, Wenwen Zhang, Han Li, Yunting Jian, Ziyuan Zhu, Xianqiu Wu, and Huanxin Lin
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Breast cancer ,ALG3 ,Radioresistance ,Stemness ,Glycosylation ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Radiotherapy is a conventional and effective local treatment for breast cancer. However, residual or recurrent tumors appears frequently because of radioresistance. Novel predictive marker and the potential therapeutic targets of breast cancer radioresistance needs to be investigated. Methods In this study, we screened all 10 asparagine-linked glycosylation (ALG) members in breast cancer patients’ samples by RT-PCR. Cell viability after irradiation (IR) was determined by CCK-8 assay and flow cytometry. The radiosensitivity of cell lines with different ALG3 expression was determined with the colony formation assay by fitting the multi-target single hit model to the surviving fractions. Cancer stem-like traits were assessed by RT-PCR, Western blot, and flow cytometry. The mechanisms of ALG3 influencing radiosensitivity was detected by Western blot and immunoprecipitation. And the effect of ALG3 on tumor growth after IR was verified in an orthotopic xenograft tumor models. The association of ALG3 with prognosis of breast cancer patients was confirmed by immunohistochemistry. Results ALG3 was the most significantly overexpressing gene among ALG family in radioresistant breast cancer tissue. Overexpression of ALG3 predicted poor clinicopathological characteristics and overall survival (OS), and early local recurrence-free survival (LRFS) in breast cancer patients. Upregulating ALG3 enhanced radioresistance and cancer stemness in vitro and in vivo. Conversely, silencing ALG3 increased the radiosensitivity and repressed cancer stemness in vitro, and more importantly inhibition of ALG3 effectively increased the radiosensitivity of breast cancer cells in vivo. Mechanistically, our results further revealed ALG3 promoted radioresistance and cancer stemness by inducing glycosylation of TGF-β receptor II (TGFBR2). Importantly, both attenuation of glycosylation using tunicamycin and inhibition of TGFBR2 using LY2109761 differentially abrogated the stimulatory effect of ALG3 overexpression on cancer stemness and radioresistance. Finally, our findings showed that radiation played an important role in preventing early recurrence in breast cancer patients with low ALG3 levels, but it had limited efficacy in ALG3-overexpressing breast cancer patients. Conclusion Our results suggest that ALG3 may serve as a potential radiosensitive marker, and an effective target to decrease radioresistance by regulating glycosylation of TGFBR2 in breast cancer. For patients with low ALG3 levels, radiation remains an effective mainstay therapy to prevent early recurrence in breast cancer.
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- 2021
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12. Progress on Rapidly and Self-Assembly Magnetically Responsive Photonic Crystals With High Tunability and Stability
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Nan Liu, Lugang Deng, Peixi Wang, Shuge Tang, Pinle Li, Caiqin Wang, Ya Li, Ravikumar Ayyanu, and Fengjiao Zheng
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magnetically responsive photonic crystals ,self-assembly ,magnetic particles ,magnetic nanoparticles ,external magnetic field ,Technology - Abstract
Magnetically induced self-assembling is considered a novel method to form photonic crystals (PCs) by the directive arrangement of nanoparticles (NPs) under a magnetic field. Magnetically responsive PCs (MRPCs) have become one of the most promising materials due to their adjustable bandgap along with the field intensity and direction, and rapid and reversible response. In this paper, we review the basic principles of MRPCs, the research progress of magnetically induced self-assembling PCs including synthesis and modification of magnetically induced NPs, the formation of an ordered structure of MRPCs, the non-spherical materials self-assemble into PC structure, and the non-magnetic materials self-assembling into PC structure. And then we also summarize the regulatory factors of the physical and chemical responses under magnetic field, and give an outlook as to the applications of MRPCs.
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- 2022
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13. Clonorchis sinensis Granulin Promotes Malignant Transformation of Hepatocyte Through EGFR-Mediated RAS/MAPK/ERK and PI3K/Akt Signaling Pathways
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Caiqin Wang, Qing He, Yingxuan Yin, Yinjuan Wu, and Xuerong Li
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Clonorchis sinensis granulin (CsGRN) ,hepatocyte ,malignant transformation ,RAS/MAPK/ERK signaling pathways ,PI3K/Akt signaling pathways ,Microbiology ,QR1-502 - Abstract
The biological functions of growth factor, such as granulins, have been explored in parasites, and we elucidated that Clonorchis sinensis granulin (CsGRN) promoted the metastasis of hepatocellular carcinoma in our previous study. However, it is still unclear for the malignant transformation role of CsGRN in normal human hepatocytes. In this study, by transfecting pEGFP-C1-CsGRN eukaryotic expression plasmid, a cell line with stable overexpression of CsGRN in normal hepatocyte (LO2-GRN cells) was constructed. The effects on cell proliferation were detected by carrying out cell counting kit-8 (CCK8) assay and colony formation assay. Additionally, we conducted flow cytometry analysis to determine whether the proliferation of CsGRN was due to cell cycle arrest. Subsequently, the migration ability and the invasion ability of LO2-GRN cells were evaluated through wound-healing assay and transwell assay. Meanwhile, the levels of the markers of RAS/MAPK/ERK and PI3K/Akt signaling pathways activation in LO2-GRN cells were assessed by quantitative RT-PCR and Western blot. Our results indicated that CsGRN promoted the proliferation of LO2 cells by regulating the expression of cell-cycle-related genes. Moreover, the overexpression of CsGRN regulates malignant metastasis of liver cells by inducing the upregulation of epithelial–mesenchymal transition (EMT) marker proteins. Furthermore, both mRNA and protein expression levels of p-EGFR, RAS, p-ERK, p-AKT, p-PI3K, and p-braf have been enhanced by CsGRN. These results showed that CsGRN promoted the malignant transformation of hepatocytes by regulating epidermal growth factor receptor (EGFR)-mediated RAS/MAPK/ERK and PI3K/Akt signaling pathways, which suggested that CsGRN could serve as a novel oncoprotein during Clonorchis sinensis–associated malignant transformation of hepatocytes.
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- 2021
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14. SOX2 promotes resistance of melanoma with PD-L1 high expression to T-cell-mediated cytotoxicity that can be reversed by SAHA
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Chunyan Li, Yun Huang, Xuan Li, Ruiyan Wu, Caiqin Wang, Jian Xiao, Xuemin Wang, Pengfei Kong, Jianghua Cao, Fuxue Huang, Zhiling Li, Hailiang Zhang, Jia Mai, Gongkan Feng, and Xiaofeng Zhu
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Background Immune checkpoint inhibitors (ICIs) induce better tumor regression in melanoma with programmed cell death 1 ligand 1 (PD-L1) high expression, but there has been an upsurge of failed responses. In this study, we aimed to explore the additional mechanisms possibly accounting for ICIs resistance and interventional strategies to overcome the resistance in melanoma with PD-L1 high expression.Methods Melanoma xenografts and cytotoxicity assays were used to investigate function of SOX2 in regulating antitumor immunity. The activity of the janus kinase-signal transducer and activator of transcriptions (JAK-STAT) pathway was investigated by western blots, quantitative PCR and luciferase assay. Epigenetic compounds library screen was employed to identify inhibitors that could decrease SOX2 level. The effect of histone deacetylase inhibitor SAHA in antitumor immunity alone or in combination with immunotherapy was also determined in vitro and in vivo. Prognostic impact of SOX2 was analyzed using transcriptional profiles and clinical data download from the Gene Expression Omnibus and The Cancer Genome Atlas repository.Results We uncovered a role of SOX2 in attenuating the sensitivity of melanoma cells to CD8+ T-cell killing. Mechanistically, SOX2 inhibited phosphatases suppressor of cytokine signaling 3 (SOCS3) and protein tyrosine phosphatase non-receptor type 1 (PTPN1) transcription, induced duration activation of the JAK-STAT pathway and thereby overexpression of interferon stimulated genes resistance signature (ISG.RS). By targeting the SOX2-JAK-STAT signaling, SAHA promoted the antitumor efficacy of IFNγ or anti-PD-1 in vitro and in vivo. Moreover, SOX2 was an independent prognostic factor for poor survival and resistant to anti-PD-1 therapy in melanoma with PD-L1 high expression.Conclusions Our data unveiled an additional function of SOX2 causing immune evasion of CD8+ T-cell killing through alleviating the JAK-STAT pathway and ISG.RS expression. We also provided a rationale to explore a novel combination of ICIs with SAHA clinically, especially in melanoma with PD-L1 and SOX2 high expression.
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- 2020
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15. Clonorchis sinensis granulin: identification, immunolocalization, and function in promoting the metastasis of cholangiocarcinoma and hepatocellular carcinoma
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Caiqin Wang, Huali Lei, Yanli Tian, Mei Shang, Yinjuan Wu, Ye Li, Lu Zhao, Mengchen Shi, Xin Tang, Tingjin Chen, Zhiyue Lv, Yan Huang, Xiaoping Tang, Xinbing Yu, and Xuerong Li
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Clonorchis sinensis ,Granulin ,Cholangicarcinoma ,Hepatocellular carcinoma ,Immunolocalization ,Cell migration ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Long-term infections by Clonorchis sinensis are associated with cholangitis, cholecystitis, liver fibrosis, cirrhosis, and even liver cancer. Molecules from the worm play vital roles in disease progress. In the present study, we identified and explored molecular characterization of C. sinensis granulin (CsGRN), a growth factor-like protein from C. sinensis excretory/secretory products (CsESPs). Methods The encoding sequence and conserved domains of CsGRN were identified and analysed by bioinformatics tools. Recombinant CsGRN (rCsGRN) protein was expressed in Escherichia coli BL21 (DE3). The localisation of CsGRN in adult worms and Balb/c mice infected with C. sinensis was investigated by immunofluorescence and immunohistochemistry, respectively. Stable CsGRN-overexpressed cell lines of hepatoma cells (PLC-GRN cells) and cholangiocarcinoma cells (RBE-GRN cells) were constructed by transfection of eukaryotic expression plasmid of pEGFP-C1-CsGRN. The effects on cell migration and invasion of CsGRN were assessed through the wound-healing assay and transwell assay. The levels of matrix metalloproteinase 2 and 9 (MMP2 and MMP9) in PLC-GRN or RBE-GRN cells were detected by real-time PCR (qRT-PCR). The levels of E-cadherin, vimentin, N-cadherin, zona occludens proteins (ZO-1), β-catenin, phosphorylated ERK (p-ERK) and phosphorylated AKT (p-AKT) were analysed by Western blotting. Results CsGRN, including the conserved GRN domains, was confirmed to be a member of the granulin family. CsGRN was identified as an ingredient of CsESPs. CsGRN was localised in the tegument and testes of the adult worm. Furthermore, it appeared in the cytoplasm of hepatocytes and biliary epithelium cells from infected Balb/c mouse. The enhancement of cell migration and invasion of PLC-GRN and RBE-GRN cells were observed. In addition, CsGRN upregulated the levels of vimentin, N-cadherin, β-catenin, MMP2 and MMP9, while it downregulated the level of ZO-1 in PLC-GRN/RBE-GRN cells. In total proteins of liver tissue from rCsGRN immunised Balb/c mice, vimentin level decreased, while E-cadherin level increased when compared with the control groups. Meanwhile, the levels of p-ERK reached a peak at 4 weeks post immunisation and the level of p-AKT did at 2 weeks after immunisation. Conclusions The encoding sequence and molecular characteristics of CsGRN were identified. As a member of granulin superfamily, CsGRN induced mesenchymal characteristics of PLC and RBE cells and was found to regulate the activities of the downstream molecules of the ERK and PI3K/AKT signalling pathways, which could contribute to the enhancement of cell migration and invasion.
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- 2017
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16. A Two-Step Strategy for Fabrication of Biocompatible 3D Magnetically Responsive Photonic Crystals
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Hui Liu, Caiqin Wang, Peixi Wang, Nan Liu, and Qingfeng Du
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3D ,magnetically responsive photonic crystals ,biocompatible ,superparamagnetic nanosphere ,self-assembling ,Chemistry ,QD1-999 - Abstract
Extremely stable and biocompatible 3D magnetically responsive photonic crystals (MRPCs) are successfully prepared in aqueous solution. Classic hydrothermal synthesis was applied for preparation of the Fe3O4@C core. Modified Stöber method was then employed for synthesis of the different size of Fe3O4@C@SiO2. Unlike the traditional magnetic nanoparticles, the highly negative charged superparamagnetic nanospheres (SMNs), i.e., the double-shell structure Fe3O4@C@SiO2 are capable of rapidly self-assembling into 3D MRPCs with full visible and various colors that can be periodically and reversibly tuned under different kinds of external magnetic fields (EMFs) within 1 s. The assembling behavior and mechanism of the 3D MRPCs under EMF were monitored and analyzed. The preparation is simple and the size of the SMN is easily controllable by adjusting the amount of catalyst. Compared with the previous works, the synthesized 3D MRPCs are hydrophilic, and exhibit extremely high stability after 6-month storage. To conclude, our study provides an effective two-step strategy for fabrication of biocompatible 3D MRPCs and it reveals great potentials in biological fields.
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- 2019
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17. Clonorchis sinensis adult-derived proteins elicit Th2 immune responses by regulating dendritic cells via mannose receptor.
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Lu Zhao, Mengchen Shi, Lina Zhou, Hengchang Sun, Xiaona Zhang, Lei He, Zeli Tang, Caiqin Wang, Yinjuan Wu, Tingjin Chen, Mei Shang, Xinyi Zhou, Zhipeng Lin, Xuerong Li, Xinbing Yu, and Yan Huang
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Arctic medicine. Tropical medicine ,RC955-962 ,Public aspects of medicine ,RA1-1270 - Abstract
Clonorchis sinensis (C. sinensis) is the most widespread human liver fluke in East Asia including China and Korea. Clonorchiasis as a neglected tropical zoonosis, leads to serious economic and public health burden in China. There are considerable evidences for an etiological relation between chronic clonorchiasis and liver fibrosis in human beings. Liver fibrosis is a highly conserved and over-protected response to hepatic tissue injury. Immune cells including CD4+ T cell as well as dendritic cell (DC), and pro-fibrogenic cytokines like interleukin 4 (IL-4), IL-13 have been identified as vital manipulators in liver fibrogenesis. Our previous studies had a mere glimpse of T helper type 2 (Th2) dominant immune responses as key players in liver fibrosis induced by C. sinensis infection, but little is known about the involved mechanisms in this pathological process.By flow cytometry (FACS), adult-derived total proteins of C. sinensis (CsTPs) down-regulated the expression of surface markers CD80, CD86 and major histocompatibility complex class II (MHC-II) on lipopolysaccharide (LPS) induced DC. ELISA results demonstrated that CsTPs inhibited IL-12p70 release from LPS-treated bone marrow-derived dendritic cells (BMDC). IL-10 level increased in a time-dependent manner in LPS-treated BMDCs after incubation with CsTPs. CD4+ T cells incubated with LPS-treated BMDCs plus CsTPs could significantly elevate IL-4 level by ELISA. Meanwhile, elevated expression of pro-fibrogenic mediators including IL-13 and IL-4 were detected in a co-culture system of LPS-activated BMDCs and naive T cells containing CsTPs. In vivo, CsTPs-immunized mice enhanced expression of type 2 cytokines IL-13, IL-10 and IL-4 in both splenocytes and hepatic tissue. Exposure of BMDCs to CsTPs activated expression of mannose receptor (MR) but not toll like receptor 2 (TLR2), TLR4, C-type lectin receptor DC-SIGN and Dectin-2 on the cell surface by RT-PCR and FACS. Blockade of MR almost completely reversed the capacity of CsTPs to suppress LPS-induced BMDCs surface markers CD80, CD86 and MHC-II expression, and further made these BMDCs fail to induce a Th2-skewed response as well as Th2 cell-associated cytokines IL-13 and IL-4 release in vitro.Collectively, we validated that CsTPs could suppress the maturation of BMDCs in the presence of LPS via binding MR, and showed that the CsTPs-pulsed BMDCs actively polarized naive T helper cells to Th2 cells though the production of IL-10 instead of IL-12. CsTPs endowed host with the capacity to facilitate Th2 cytokines production including IL-13 and IL-4 in vitro and vivo. The study might provide useful information for developing potential therapeutic targets against the disease.
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- 2018
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18. Facile Synthesis of MnPO4·H2O Nanowire/Graphene Oxide Composite Material and Its Application as Electrode Material for High Performance Supercapacitors
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Bo Yan, Duan Bin, Fangfang Ren, Zhiping Xiong, Ke Zhang, Caiqin Wang, and Yukou Du
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supercapacitors ,manganese phosphate ,graphene oxide ,high power density ,Chemical technology ,TP1-1185 ,Chemistry ,QD1-999 - Abstract
In this work, we reported a facile one-pot hydrothermal method to synthesize MnPO4·H2O nanowire/graphene oxide composite material with coated graphene oxide. Transmission electron microscopy and scanning electron microscope were employed to study its morphology information, and X-ray diffraction was used to study the phase and structure of the material. Additionally, X-ray photoelectron spectroscopy was used to study the elements information. To measure electrochemical performances of electrode materials and the symmetry cell, cyclic voltammetry, chronopotentiometry and electrochemical impedance spectrometry were conducted on electrochemical workstation using 3 M KOH electrolytes. Importantly, electrochemical results showed that the as-prepared MnPO4·H2O nanowire/graphene oxide composite material exhibited high specific capacitance (287.9 F·g−1 at 0.625 A·g−1) and specific power (1.5 × 105 W·kg−1 at 2.271 Wh·kg−1), which is expected to have promising applications as supercapacitor electrode material.
- Published
- 2016
- Full Text
- View/download PDF
19. Research progress on the fanconi anemia signaling pathway in nonobstructive azoospermia.
- Author
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Haohui Xu, Yixin Zhang, Caiqin Wang, Zhuoyan Fu, Jing Lv, Yufang Yang, Zihan Zhang, Yuanmin Qi, Kai Meng, Jinxiang Yuan, and Xiaomei Wang
- Subjects
FANCONI'S anemia ,AZOOSPERMIA ,DOUBLE-strand DNA breaks ,CELLULAR signal transduction ,DNA repair ,GERM cells - Abstract
Non-obstructive azoospermia (NOA) is a disease characterized by spermatogenesis failure and comprises phenotypes such as hypospermatogenesis,mature arrest, and Sertoli cell-only syndrome. Studies have shown that FA cross-linked anemia (FA) pathway is closely related to the occurrence of NOA. There are FA genemutations in male NOA patients, which cause significant damage to male germ cells. The FA pathway is activated in the presence of DNA interstrand cross-links; the key step in activating this pathway is the mono-ubiquitination of the FANCD2-FANCI complex, and the activation of the FA pathway can repair DNA damage such as DNA doublestrand breaks. Therefore, we believe that the FA pathway affects germ cells during DNA damage repair, resulting in minimal or even disappearance of mature sperm in males. This review summarizes the regulatory mechanisms of FA-related genes in male azoospermia, with the aim of providing a theoretical reference for clinical research and exploration of related genes. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
20. Affinity-based alleviation of dissolved organic matter (DOM) on tetracycline toxicity to photosynthesis of green algae Chlorella vulgaris: roles of hydrophilic and hydrophobic DOM
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Caiqin Wang, Hang Xu, Tingfeng Cheng, Shuting Tang, Daoyong Zhang, Meichao Li, and Xiangliang Pan
- Subjects
Health, Toxicology and Mutagenesis ,Environmental Chemistry ,General Medicine ,Pollution - Published
- 2023
21. 3D PdPb nanochain networks with efficient catalytic performance for ethylene glycol electrooxidation
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Bin Zou, Yangping Zhang, Fei Gao, Zhuolin Li, Caiqin Wang, Zhengying Wu, Dongqiong Wang, and Yukou Du
- Subjects
Fuel Technology ,Renewable Energy, Sustainability and the Environment ,Energy Engineering and Power Technology ,Condensed Matter Physics - Published
- 2022
22. Hierarchical AgAu alloy nanostructures for highly efficient electrocatalytic ethanol oxidation
- Author
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Caiqin Wang, Danil Bukhvalov, M. Cynthia Goh, Yukou Du, and Xiaofei Yang
- Subjects
General Medicine - Published
- 2022
23. Ultra-low Ru doped MOF-derived hollow nanorods for efficient oxygen evolution reaction
- Author
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Dongmei Liu, Cheng Wang, Zhengming Zhou, Changqing Ye, Rui Yu, Caiqin Wang, and Yukou Du
- Subjects
Inorganic Chemistry - Abstract
Facile reduction and doping methods have been proposed for the synthesis of Ru@CoFe/D-MOFs with enhanced conductivity, increased active sites and optimized electronic structures for efficient water splitting.
- Published
- 2022
24. Metal-Support Interaction Promoted Multifunctional Electrocatalysis on Ptco/Nc with Ultralow Pt Loading for Oxygen Reduction Reaction and Zinc-Air Battery
- Author
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Manting Zhang, Tingting Zhou, D. W. Boukhvalov, Fengyan Han, Caiqin Wang, and Xiaofei Yang
- Published
- 2023
25. Combined tumor‐associated macrophages biomarker predicting extremely poor outcome of patients with primary central nervous system lymphoma
- Author
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Peng Sun, Xianqiu Wu, Jianghua Cao, Yu Wang, Xiaohua He, Cui Chen, Wenqi Jiang, Caiqin Wang, Xiao-Qing Sun, Zhi Ming Li, and Jia Jia Huang
- Subjects
Male ,Oncology ,Cancer Research ,medicine.medical_specialty ,Poor prognosis ,Lymphoma ,Malignancy ,B7-H1 Antigen ,Programmed cell death ligand 1 ,Central Nervous System Neoplasms ,hemic and lymphatic diseases ,Internal medicine ,PD-L1 ,Tumor-Associated Macrophages ,Biomarkers, Tumor ,Tumor Microenvironment ,medicine ,Humans ,Retrospective Studies ,Receiver operating characteristic ,biology ,business.industry ,Primary central nervous system lymphoma ,Hematology ,General Medicine ,Middle Aged ,Prognosis ,medicine.disease ,Survival Rate ,Improved performance ,biology.protein ,Biomarker (medicine) ,Female ,business ,Follow-Up Studies - Abstract
Primary central nervous system lymphoma (PCNSL) is an aggressive and rare malignancy with poor prognosis. However, there are no reliable prognostic biomarkers for PCNSL in clinical practice. Here, we aimed to identify a reliable prognostic biomarker for predicting the survival of PCNSL patients. In this study, multiplex immunofluorescence and digital imaging analysis were used to characterize tumor-associated macrophages (TAMs) immunophenotypes and the expression of programmed cell death ligand 1 on TAMs, with regard to prognosis from diagnostic tumor tissue samples of 59 PCNSL patients. We found that the M2-to-M1 ratio was a more reliable prognostic biomarker for PCNSL than M1-like or M2-like macrophage infiltration. In addition, the combination of programmed death-ligand 1 (PD-L1) expression on TAMs and the M2-to-M1 ratio in PCNSL demonstrated improved performance in prognostic discrimination than PD-L1-positive TAMs or M2-to-M1 ratio. To validate the prognostic significance of the combined TAMs associated biomarkers, they were integrated into the International Extranodal Lymphoma Study Group (IELSG) index and termed as IELSG-M index. Kaplan-Meier plots showed that the IELSG-M index could discriminate patients into low-, intermediate- or high-risk subgroups, better than IELSG, in terms of prognosis. The areas under the receiver operating characteristic curves of IELSG-M was 0.844 for overall survival; superior to the IELSG model (0.580). Taken together, this study's findings showed that the combination of PD-L1 on TAMs and the M2-to-M1 ratio could be strong prognostic predictive biomarkers for PCNSL and the IELSG-M index had improved prognostic significance than the IELSG index.
- Published
- 2021
26. Electrochemical properties of humic acid and its novel applications: A tip of the iceberg
- Author
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Caiqin Wang, Tingfeng Cheng, Daoyong Zhang, and Xiangliang Pan
- Subjects
Environmental Engineering ,Environmental Chemistry ,Pollution ,Waste Management and Disposal - Abstract
The widely existed humic acid (HA) with abundant redox-active groups has been considered to play an important role in biogeochemistry in sediments and soils. Recent studies reported that HA showed great performance in terms of electron transfer capacity (up to HA
- Published
- 2022
27. Effects of PFOS at ng/L levels on photostability of extracellular polymeric substances under solar irradiation by fluorescence and infrared spectroscopy
- Author
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Shuyan Xu, Yitian Zhu, Pengfeng Zhu, Caiqin Wang, Daoyong Zhang, and Xiangliang Pan
- Subjects
Environmental Engineering ,Extracellular Polymeric Substance Matrix ,Spectrum Analysis ,Environmental Chemistry ,Water ,Pollution ,Waste Management and Disposal - Abstract
The ubiquitous EPS (extracellular polymeric substances), as a type of dissolved organic carbon, plays a key role in carbon cycling in water environment. When EPS meet the omnipresent PFOS (perfluorooctane sulfonate), they must interact with each other and exert profound effect on behavior and fate of both, which is still not well known. We hypothesized that the highly persistent PFOS at real environmental levels may significantly influence behavior of EPS under solar irradiation which may retard carbon turnover. In this study, 3D-EEM fluorescence spectroscopy and FTIR spectroscopy were used to probe responses of composition and structure of EPS under solar irradiation in the absence and presence of PFOS (5-500 ng/L). The experimental results showed that PFOS at ng/L levels significantly affected responses of EPS to sunlight irradiation and the effects were dependent on the components in EPS. Photostability of humic-like substances was significantly increased in the presence of PFOS; Degradation and unfolding of proteins induced by solar light were reduced by PFOS. In addition, degradation of both hydrophilic and hydrophobic functional groups by sunlight was inhibited by PFOS. The novel findings provide new insights for assessing the environmental behavior of EPS and PFOS and understanding the effect of PFOS on carbon cycling in water environments.
- Published
- 2022
28. Temporospatial nano-heterogeneity of self-assembly of extracellular polymeric substances on microplastics and water environmental implications
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Shuyan Xu, Caiqin Wang, Pengfeng Zhu, Daoyong Zhang, and Xiangliang Pan
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Environmental Engineering ,Extracellular Polymeric Substance Matrix ,Health, Toxicology and Mutagenesis ,Microplastics ,Environmental Chemistry ,Water ,Environmental Pollutants ,Polypropylenes ,Pollution ,Waste Management and Disposal ,Plastics ,Water Pollutants, Chemical - Abstract
Environmental behavior and ecotoxicity of microplastics (MPs) are significantly influenced by the omnipresent self-assembly of microbial extracellular polymeric substances (EPS) on them. However, mechanisms of EPS self-assembly onto MPs at nanoscale resolution and effects of aging are unclear. For the first time, temporospatial nano-heterogeneity of self-assembly of EPS onto fresh and one-year aged polypropylene (PP) MPs were investigated by atomic-force-microscopy-infrared-spectroscopy (AFM-IR). Natural aging caused high degree nanoscale fragmentation of MPs physically and chemically. Self-assembly of EPS on MPs was aging-dependent. Polysaccharides were assembled on MP surface faster than proteins. Initially, regardless of the fresh or aged MPs, polysaccharides and proteins, with the former being predominant, were successively and separately assembled to different nanospaces because of their competition for binding sites. More and more proteins and polysaccharides were superimposed on each other with assembly time due to intermolecular forces. The nanochemical textural analysis showed that the nano-heterogeneity of EPS assembly to MPs was clearly correlated with the aging-induced nanochemical and nanomechanical heterogeneity of MP surface. The spontaneous self-assembly of EPS with temporospatial nano-heterogeneity on MPs have multiple impacts on behavior, ecotoxicity and fate of MPs and their associated pollutants as well as other key ecological processes in aquatic environment.
- Published
- 2022
29. ALG3 contributes to stemness and radioresistance through regulating glycosylation of TGF-β receptor II in breast cancer
- Author
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Zhen-Yu He, Wen Wen, Zhi-Qing Long, Chao Lin, Ling Guo, Meisongzhu Yang, Wen-Wen Zhang, Xiaoqing Wang, Sai-Lan Liu, Liping Ye, Xianqiu Wu, Huan-Xin Lin, Yunting Jian, Xiao-Qing Sun, Chuyong Lin, Caiqin Wang, Han Li, Yue Li, Ziyuan Zhu, and Xin Hua
- Subjects
0301 basic medicine ,Cancer Research ,Glycosylation ,medicine.medical_treatment ,Breast Neoplasms ,Mannosyltransferases ,Radiation Tolerance ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Breast cancer ,Radioresistance ,Cell Line, Tumor ,medicine ,Gene silencing ,Animals ,Humans ,Viability assay ,Radiosensitivity ,Stemness ,RC254-282 ,Predictive marker ,business.industry ,Research ,Receptor, Transforming Growth Factor-beta Type II ,Cancer ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,Xenograft Model Antitumor Assays ,Radiation therapy ,ALG3 ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,Cancer research ,Female ,business - Abstract
Background Radiotherapy is a conventional and effective local treatment for breast cancer. However, residual or recurrent tumors appears frequently because of radioresistance. Novel predictive marker and the potential therapeutic targets of breast cancer radioresistance needs to be investigated. Methods In this study, we screened all 10 asparagine-linked glycosylation (ALG) members in breast cancer patients’ samples by RT-PCR. Cell viability after irradiation (IR) was determined by CCK-8 assay and flow cytometry. The radiosensitivity of cell lines with different ALG3 expression was determined with the colony formation assay by fitting the multi-target single hit model to the surviving fractions. Cancer stem-like traits were assessed by RT-PCR, Western blot, and flow cytometry. The mechanisms of ALG3 influencing radiosensitivity was detected by Western blot and immunoprecipitation. And the effect of ALG3 on tumor growth after IR was verified in an orthotopic xenograft tumor models. The association of ALG3 with prognosis of breast cancer patients was confirmed by immunohistochemistry. Results ALG3 was the most significantly overexpressing gene among ALG family in radioresistant breast cancer tissue. Overexpression of ALG3 predicted poor clinicopathological characteristics and overall survival (OS), and early local recurrence-free survival (LRFS) in breast cancer patients. Upregulating ALG3 enhanced radioresistance and cancer stemness in vitro and in vivo. Conversely, silencing ALG3 increased the radiosensitivity and repressed cancer stemness in vitro, and more importantly inhibition of ALG3 effectively increased the radiosensitivity of breast cancer cells in vivo. Mechanistically, our results further revealed ALG3 promoted radioresistance and cancer stemness by inducing glycosylation of TGF-β receptor II (TGFBR2). Importantly, both attenuation of glycosylation using tunicamycin and inhibition of TGFBR2 using LY2109761 differentially abrogated the stimulatory effect of ALG3 overexpression on cancer stemness and radioresistance. Finally, our findings showed that radiation played an important role in preventing early recurrence in breast cancer patients with low ALG3 levels, but it had limited efficacy in ALG3-overexpressing breast cancer patients. Conclusion Our results suggest that ALG3 may serve as a potential radiosensitive marker, and an effective target to decrease radioresistance by regulating glycosylation of TGFBR2 in breast cancer. For patients with low ALG3 levels, radiation remains an effective mainstay therapy to prevent early recurrence in breast cancer.
- Published
- 2021
30. Protective-mechanism-exclusive thermal stability modes of soil organic matter: Novel implication for wildfire effect on soil organic carbon
- Author
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Caiqin, Wang, Tingfeng, Cheng, Shuting, Tang, Mengxuan, Li, Daoyong, Zhang, and Xiangliang, Pan
- Subjects
Environmental Engineering ,Environmental Chemistry ,Pollution ,Waste Management and Disposal - Abstract
Stability of soil organic matter (SOM) is considered to be governed by different protection mechanisms including physical protection (PP), biochemical protection (BCP) and physical plus biochemical protection (PBCP). The thermostability of SOM protected by different mechanisms is unknown, despite its importance for understanding the stability of soil organic carbon (SOC) under frequently occurred wildfires. In this study, we reported for the first time that pyrolysis of SOM under different protection mechanisms in three types of soil (shrub soil, cultivated soil, and meadow soil) followed their own distinct modes regardless of soil type. Specifically, SOM-PP, SOM-PBCP and SOM-BCP from each type of soil were pyrolyzed in the double-step-shape, the mono-step-shape and the linear modes, respectively, when they were heated from room temperature to 800 °C by thermogravimeter. There were more thermolabile organic fractions (pyrolysis temperature200 °C) enriched in SOM-PP, while more thermostable organic fractions enriched in SOM-BCP and SOM-PBCP. These findings are of great importance for deeper insight into stability responses of SOM with different occurrence of wildfire.
- Published
- 2023
31. Different Routes for Direct Interspecies Electron Transfer with Diverse Electron-Accepting Partners
- Author
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Dawn E. Holmes, Jinjie Zhou, Jessica A. Smith, Caiqin Wang, Xinying Liu, and Derek R. Lovley
- Abstract
Direct interspecies electron transfer (DIET) may be most important in methanogenic environments, but studies to date that have examined the mechanisms for electron transfer from electron-donating partners have primarily focused on co-cultures in which fumarate was the terminal electron acceptor. To better understand DIET with methanogens, the transcriptome of Geobacter metallireducens during DIET-based growth with Geobacter sulfurreducens reducing fumarate was compared with the transcriptome of G. metallireducens grown in co-culture with diverse Methanosarcina species. The transcriptome of G. metallireducens co-cultured with G. sulfurreducens was significantly different from those with Methanosarcina. Furthermore, the transcriptome of G. metallireducens grown with Methanosarcina barkeri, which lacks outer-surface cytochromes, was different from the transcriptome of G. metallireducens co-cultured with Methanosarcina acetivorans or Methanosarcina subterranea, which have an outer-surface c-type cytochrome that serves as an electrical connect for DIET. Differences in G. metallireducens expression patterns for c-type cytochrome genes were particularly notable. The impact of deleting G. metallireducens c-type cytochrome genes depended upon the electron-accepting partner. Multiple cytochromes that G. metallireducens required for DIET with G. sulfurreducens and for Fe(III) oxide reduction were not required for DIET with the Methanosarcina species. Porin-cytochrome complexes and e-pili were required for optimal DIET with all partners. Shewanella oneidensis, another electroactive microbe with abundant outer-surface c-type cytochromes, did not grow via DIET. The results demonstrate that the presence of outer-surface cytochromes does not necessarily confer the capacity for DIET, and also emphasize the substantial impact of the physiology of the electron-accepting partner on the physiology of the electron-donating DIET partner.
- Published
- 2022
32. Chemical structure and nanomechanics relevant electrochemistry of solid-phase humic acid along a typical forest-river-paddy landscape section in eastern China and its environmental implications
- Author
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Caiqin Wang, Tingfeng Cheng, Shuting Tang, Mengxuan Li, Daoyong Zhang, and Xiangliang Pan
- Subjects
China ,Soil ,Environmental Engineering ,Rivers ,Electrochemistry ,Environmental Chemistry ,Humans ,Forests ,Pollution ,Waste Management and Disposal ,Carbon ,Humic Substances - Abstract
Solid-phase humic acid (HA
- Published
- 2022
33. Tolerance of Aceticlastic Methanogenesis Enhanced by Magnetite under the Condition of Ammonia Stress
- Author
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Liang Zhu, Qiujin Xu, Zixian Han, Chen Wang, Jieyi Liu, Caiqin Wang, and Xiangyang Xu
- Subjects
animal structures ,biology ,Renewable Energy, Sustainability and the Environment ,Methanogenesis ,General Chemical Engineering ,02 engineering and technology ,General Chemistry ,Methanosarcina ,Metabolism ,010402 general chemistry ,021001 nanoscience & nanotechnology ,biology.organism_classification ,01 natural sciences ,0104 chemical sciences ,Stress (mechanics) ,Ammonia ,chemistry.chemical_compound ,Electron transfer ,chemistry ,Biophysics ,Environmental Chemistry ,0210 nano-technology ,Magnetite - Abstract
Magnetite-mediated direct interspecies electron transfer (DIET) has been considered as an effective mechanism to proceed the syntrophic methanogenic metabolism. However, the enhancement effect of m...
- Published
- 2020
34. Porous Pt–Rh–Te nanotubes: an alleviated poisoning effect for ethanol electrooxidation
- Author
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Yukou Du, Hui Xu, Chunyan Chen, Yong Wang, Hongyuan Shang, Liujun Jin, and Caiqin Wang
- Subjects
Inorganic Chemistry ,Metal ,Materials science ,Nanostructure ,Chemical engineering ,visual_art ,visual_art.visual_art_medium ,Nanowire ,Porosity ,Electrocatalyst ,Catalysis ,Anode ,Template method pattern - Abstract
Extraordinary attempts have been dedicated to increase the utilization efficiency and catalytic performance and decrease the economy issues related to Pt-based electrocatalysts for fuel cells due to their daunting price, extremely low earth abundance and unsatisfactory catalytic performance for practical applications in the past few decades. Recently, our group successfully synthesized novel one-dimensional (1D) Pt–Rh–Te nanotubes (NTs) as anodic electrocatalysts via a sacrificial template method. The typical 1D nanotubes that comprise porous walls and hollow interiors could be achieved by using PtCl62− and Rh3+ as the metal precursors and Te nanowires as the reductive template in this project. By integrating the multiple compositional and structural merits into one catalyst, more significantly, the mass activities of the as-resulted Pt–Rh–Te NTs/C catalyst were calculated to be 5.08 and 2.98-fold higher than those of commercial Pt/C catalysts and PtTe NTs/C catalysts, respectively. Apart from the excellent catalytic activity when compared with that of the commercial Pt/C catalysts, the optimized Pt–Rh–Te NT electrocatalyst exhibited substantially enhanced long-term stability owing to the combination of a hollow tubular nanostructure and extra incorporation of the Rh element. This work probably provides a general orientation to design and fabricate cost-efficient low-dimensional electrocatalysts.
- Published
- 2020
35. Amorphous/Crystalline Heterostructure Transition-Metal-based Catalysts for High-Performance Water Splitting
- Author
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Yangping Zhang, Fei Gao, Dongqiong Wang, Zhuolin Li, Xiaomei Wang, Caiqin Wang, Kewang Zhang, and Yukou Du
- Subjects
Inorganic Chemistry ,Materials Chemistry ,Physical and Theoretical Chemistry - Published
- 2023
36. Environmental concentration PFOS as a light shield for lake exopolymers against photodegradation by formation of sandwiched supramolecular nanostructures
- Author
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Shuyan, Xu, Pengfeng, Zhu, Caiqin, Wang, Daoyong, Zhang, and Xiangliang, Pan
- Subjects
Lakes ,Photolysis ,Environmental Engineering ,Extracellular Polymeric Substance Matrix ,Food ,Ecological Modeling ,Pollution ,Waste Management and Disposal ,Nanostructures ,Water Science and Technology ,Civil and Structural Engineering - Abstract
EPS (exopolymers) play a central role in global carbon cycling due to huger amount in aquatic environment, and PFOS (Perfluorooctane sulfonate) is also ubiquitous and persistent pollutant. Whether and how can PFOS of environmental concentrations affect behavior and fate of EPS was unclear. In this study, for the first time interaction between lake EPS and PFOS of environmental concentrations was visually probed by AFM-IR technique. It was found that EPS could effectively trap PFOS and the latter of environmental concentrations could trigger nanoscale reassembly of the former. Sandwiched PFOS-EPS nanostructures were formed via supramolecular interaction between EPS and PFOS, confirmed by fluorescence quenching titration and FTIR spectroscopy. The PFOS microlayers sandwiched in EPS was proven to be a light shield that could protect EPS from photodegradation because of its high reflectivity and nearly zero absorbance of UV-Vis light. The light shielding effect of PFOS was confirmed by evidences from photodegradation experiments, including change of concentrations of ions released and molecular weight distribution patterns. These novel findings provided valuable information for deep insight into environmental behavior of PFOS and its effects on biogeochemical carbon cycle of biopolymers in global waters.
- Published
- 2022
37. Author response for 'Combined tumor‐associated macrophages biomarker predicting extremely poor outcome of patients with primary central nervous system lymphoma'
- Author
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Yu Wang, Xianqiu Wu, Peng Sun, Zhi Ming Li, Jia Jia Huang, Xiaoqing Sun, Caiqin Wang, Wenqi Jiang, Jianghua Cao, Xiaohua He, and Cui Chen
- Subjects
Oncology ,Extremely Poor ,medicine.medical_specialty ,business.industry ,Internal medicine ,medicine ,Primary central nervous system lymphoma ,Biomarker (medicine) ,medicine.disease ,business - Published
- 2021
38. Enhancing solar-driven photoelectrocatalytic efficiency of Au nanoparticles with defect-rich hydrogenated TiO2 toward ethanol oxidation
- Author
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Caiqin Wang, Cheng Qian, Ting Hu, and Xiaofei Yang
- Subjects
History ,Polymers and Plastics ,General Chemical Engineering ,Environmental Chemistry ,General Chemistry ,Business and International Management ,Industrial and Manufacturing Engineering - Published
- 2022
39. Monodispersed bimetallic platinum-copper alloy nanospheres as efficient catalysts for ethylene glycol electrooxidation
- Author
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Chunyan Chen, Cheng Wang, Hongyuan Shang, Tongxin Song, Liujun Jin, Fei Gao, Yangping Zhang, Yukou Du, and Caiqin Wang
- Subjects
Horizontal scan rate ,Materials science ,chemistry.chemical_element ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Redox ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Catalysis ,Anode ,Biomaterials ,chemistry.chemical_compound ,Colloid and Surface Chemistry ,chemistry ,Chemical engineering ,Cyclic voltammetry ,0210 nano-technology ,Platinum ,Ethylene glycol ,Bimetallic strip - Abstract
Designing and fabricating highly active and efficient catalysts are of vital importance for the practical applications of direct ethylene glycol fuel cells (DEGFCs). In this study, we employ a feasible one-pot synthetic method to construct highly monodispersed PtCu nanospheres (NSs) as high-efficiency anode electrocatalysts for DEGFCs. Interestingly, the optimized carbon supported Pt1Cu1 NSs can display the highest mass activity of 2146.9 mA mg−1 in 1 M KOH + 1 M EG solution under the scan rate of 50 mV s−1, which is 1.9 times higher than that of commercial Pt/C catalysts. This is ascribed to the favorable electronic effect between Pt and Cu, which is beneficial for ethylene glycol oxidation reaction (EGOR) in fuel cells. Meanwhile, such monodispersed Pt1Cu1 NSs can also exhibit excellent durability, where the Pt1Cu1 catalyst retains 62.6% of the initial value after the cyclic voltammetry of 500 cycles. This work not only provides a significant approach for designing catalysts for fuel cells, but also constructs a novel class of active and stable electrocatalysts for EGOR.
- Published
- 2019
40. Shape-controlled PdSn alloy as superior electrocatalysts for alcohol oxidation reactions
- Author
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Jun Guo, Ping'er Yu, Shujin Li, Caiqin Wang, Tongxin Song, Yukihide Shiraishi, Fei Gao, Yukou Du, Cheng Wang, and Yangping Zhang
- Subjects
Materials science ,Reducing agent ,General Chemical Engineering ,Nanoparticle ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Redox ,Nanomaterial-based catalyst ,0104 chemical sciences ,Catalysis ,chemistry.chemical_compound ,Chemical engineering ,chemistry ,Alcohol oxidation ,0210 nano-technology ,Bimetallic strip ,Ethylene glycol - Abstract
At present, it is still a great challenge to control the morphology and size of nanoparticles for obtaining more efficient and stable catalysts for direct fuel cell reactions. Herein, we have synthesized a series of Pd3Sn bimetallic catalysts with different morphologies that transitioned from nanonetwork to nanostrips and then to nanodots by altering the varieties of reducing agents and the amount of surfactant. And the formation mechanisms of nanocatalysts were also investigated. It is worth noting that Pd3Sn-dots, due to the unique structure, uniform dispersion and ultra-small size, exhibit excellent electrocatalytic activity for ethanol oxidation reaction and ethylene glycol oxidation reaction, which distinctly higher than the other Pd3Sn catalysts and commercial Pd/C catalysts. Moreover, the as-prepared Pd3Sn catalysts also performed greater long-term durability due to the electronic and synergistic effects. This work provides guidance on the morphology-dependent nanocrystals, which will show a great potential as anodic catalysts in direct fuel cell applications.
- Published
- 2019
41. Engineering Spiny PtFePd@PtFe/Pt Core@Multishell Nanowires with Enhanced Performance for Alcohol Electrooxidation
- Author
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Yukihide Shiraishi, Yukou Du, Fei Gao, Yangping Zhang, and Caiqin Wang
- Subjects
Alcohol fuel ,Materials science ,Nanowire ,Alcohol ,Core (manufacturing) ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Catalysis ,chemistry.chemical_compound ,Key point ,chemistry ,Chemical engineering ,General Materials Science ,0210 nano-technology ,Ethanol oxidation reaction - Abstract
Engineering robust electrocatalysts is always a key point in direct alcohol fuel cells. Catalysts with a one-dimension (1D) structure are well studied and considered as promising candidates among various catalysts in the past decades; however, the precise regulation on the surface structure of 1D nanomaterials is still a worthy subject. By creatively introducing a trimetallic nanoalloy, core@multishell structure, and 1D nanowire (NW) morphology, we have constructed a kind of novel spiny PtFePd@PtFe/Pt core@multishell 1D NW catalysts with PtFePd as the core and PtFe/Pt as the multishell on the basis of improving catalytic property. The composition-optimized Pt
- Published
- 2019
42. The chain-typed nanoflowers structure endows PtBi with highly electrocatalytic activity of ethylene glycol oxidation
- Author
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Fei Gao, Cheng Wang, Pingping Song, Caiqin Wang, Jun Guo, Tongxin Song, Yangping Zhang, Yukou Du, and Jin Wang
- Subjects
Materials science ,Mechanical Engineering ,Metals and Alloys ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Mass activity ,Redox ,Nanomaterial-based catalyst ,0104 chemical sciences ,Catalysis ,Corrosion ,Anode ,chemistry.chemical_compound ,chemistry ,Chemical engineering ,Mechanics of Materials ,Materials Chemistry ,Fuel cells ,0210 nano-technology ,Ethylene glycol - Abstract
Pursuing high-effective electrocatalysts is of paramount importance yet a great challenge, drawbacks such as irregular morphology, weak corrosion resistance, and poor anti-poisoning that limited their catalytic performance. Kinds of strategies have been developed to synthesize nanocatalysts with properties of low-cost, high-performance, and long lifetime. Pt-based catalyst is one of the most effective anodic materials for direct fuel cells (DFCs), especially towards ethylene glycol oxidation reaction (EGOR). Herein, we integrate the advantages of both composition and morphology to finally synthesize a series of PtBi chain-typed nanoflowers (NFs) serving as high-efficiency anode catalysts. The Pt1Bi1 chain-typed NFs show much higher catalytic performances than Pt1Bi2, Pt2Bi1, and commercial Pt/C catalysts with the mass activity (1.17, 1.64, and 5.71 times higher, respectively) and retained mass activities (1.68, 3.26, and 13.1 times higher, respectively). Our present work opens a new way to promote the electrocatalytic activity and stability via synthesizing well-defined PtBi NFs with chain-typed structure. We also believe that the as-obtained PtBi NFs can be one of the candidates as promising anodic catalysts in DFCs.
- Published
- 2019
43. Self-template construction of Sub-24 nm Pd Ag hollow nanodendrites as highly efficient electrocatalysts for ethylene glycol oxidation
- Author
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Pingping Song, Yukou Du, Jin Wang, Fei Gao, Caiqin Wang, Jun Guo, and Yangping Zhang
- Subjects
Materials science ,Nanostructure ,Renewable Energy, Sustainability and the Environment ,Kinetics ,Energy Engineering and Power Technology ,Nanotechnology ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Redox ,Nanomaterial-based catalyst ,0104 chemical sciences ,Catalysis ,Anode ,chemistry.chemical_compound ,chemistry ,Fuel cells ,Electrical and Electronic Engineering ,Physical and Theoretical Chemistry ,0210 nano-technology ,Ethylene glycol - Abstract
The development of efficient anode nanocatalysts with high performance for direct ethylene glycol fuel cells is highly desirable and still difficult due to the sluggish kinetics at the anode. For that reason, it is necessary to engineer cost-effective Pd-based catalysts with fascinating structure and superior activity. Inspired by this, we shed light on a facile self-template one-pot approach to synthesize sub-24 nm PdAg hollow nanodendrites with tunable compositions. More importantly, owing to the exquisite hollow nanostructure and electronic effect, as well as synergistic role, the as-prepared unique binary PdAg nanocatalysts show outstanding mass activity of 4168.8 mA mgPd−1 for ethylene glycol oxidation reaction under the alkaline medium, 3.8-fold enhancements than that of commercial Pd/C catalysts. Remarkably, based on this facile synthesis method and superior electrocatalytic performance of as-prepared catalysts, we believe that our efforts will open a new way to promote the commercialization of fuel cells to some extent.
- Published
- 2019
44. Sensitive detection of caffeic acid with trifurcate PtCu nanocrystals modified glassy carbon electrode
- Author
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Yuting Shi, Zhulan Gu, Yukou Du, Caiqin Wang, and Hui Xu
- Subjects
Detection limit ,Materials science ,Glassy carbon electrode ,02 engineering and technology ,Electrochemical detection ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Electrochemistry ,01 natural sciences ,Nanomaterial-based catalyst ,0104 chemical sciences ,chemistry.chemical_compound ,Colloid and Surface Chemistry ,chemistry ,Chemical engineering ,Nanocrystal ,Linear range ,Caffeic acid ,0210 nano-technology - Abstract
Electrochemical property and detection performance of nanocatalysts are largely affected by the component and morphology. Here, an advanced class of PtCu nanocrystal with uniform trifurcate structure has been synthesized by using KI as the structure-directing agent during the reduction process. Due to the large surface area of such synthesized dendritic trifurcate nanocrystal and the synergistic effect between Pt and Cu, this nanocatalyst performed nicely when applied it to the electrochemical detection of caffeic acid. Specifically, the detection limit is 0.35 μM (S/N = 3) and the linear range is wide from 1.2 μM-1.9 mM. The finding may be beneficial for developing novel and high-performance sensors for the monitoring of dietetical and environmental safety.
- Published
- 2019
45. A study of the coupled bioelectrochemical system-upflow anaerobic sludge blanket for efficient transformation of 2,4-dichloronitrobenzene
- Author
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Hui Chen, Liang Zhu, Linlin Chen, Donghui Lu, Caiqin Wang, and Xiangyang Xu
- Subjects
Pollutant ,Salinity ,Halogenation ,Sewage ,Chemistry ,Health, Toxicology and Mutagenesis ,General Medicine ,Wastewater ,010501 environmental sciences ,Blanket ,Pulp and paper industry ,01 natural sciences ,Pollution ,Extracellular polymeric substance ,Chlorides ,Electricity ,Reductive dechlorination ,Bioreactor ,Environmental Chemistry ,Sewage treatment ,Water treatment ,Nitrobenzenes ,0105 earth and related environmental sciences - Abstract
Coupled bioelectrochemical system-upflow anaerobic sludge blanket (BES-UASB) was utilized for wastewater treatment containing 2,4-dichloronitrobenzene (DClNB). The results indicated that a proper voltage enhanced the DClNB reduction, however, over high voltage presented a negative impact (2.0 V). Synergistic effect of external voltage and anaerobic sludge was observed, and dechlorination efficiency reached 57.8 ± 5.4% in the coupled BES, which was higher than the sum of anaerobic sludge and electric system (48.2%). Moreover, the coupled system was more tolerant of high salinity and pollutant concentration. Dehydrogenase activity (DHA) was related to microbial electron transfer activity and DHA reached a maximum 453 ± 33 μgTF g−1VSS h−1 in the coupled reactor which was 1.6-fold that of the control, meanwhile, extracellular polymeric substances (EPS) content was significantly enhanced in the presence of external voltage. In summary, the coupled BES-UASB systems could be an alternative for removal of recalcitrant pollutants such as DClNB.
- Published
- 2019
46. A short-term stimulation of ethanol enhances the effect of magnetite on anaerobic digestion
- Author
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Liang Zhu, Xiangyang Xu, Hui Chen, Weilong Qiao, and Caiqin Wang
- Subjects
Sucrose ,Applied Microbiology and Biotechnology ,Electron Transport ,03 medical and health sciences ,chemistry.chemical_compound ,Bioreactors ,Extracellular polymeric substance ,Syntrophy ,Anaerobiosis ,Food science ,030304 developmental biology ,Magnetite ,0303 health sciences ,Ethanol ,Sewage ,biology ,030306 microbiology ,Chemistry ,Pyridoxine ,Chloroflexi ,General Medicine ,Methanosarcina ,biology.organism_classification ,Ferrosoferric Oxide ,Anaerobic digestion ,Anaerobic exercise ,Bacteria ,Biotechnology - Abstract
Conductive iron oxides (CIO) have been proved recently to facilitate the anaerobic microbial syntrophy based on the direct interspecies electron transfer (DIET) in batch experiments. However, the effect of CIO was always insignificant in anaerobic digestion (AD) reactor especially when the DIET-based syntrophic partners were absent. In this study, the effect of magnetite on performance of AD system with sucrose as a sole carbon source was investigated, but limited enhancement was achieved during the first 36-day operation. The short-term effect of ethanol addition was further studied in the magnetite-amended AD reactor, and results showed that the AD reactor with 10gFe/L micro-sized magnetite (R3) achieved higher performance of COD removal and methane proportion compared with the other reactors (R1 without magnetite; R2 with 2gFe/L micro-sized magnetite; R4 with 2gFe/L nano-sized magnetite). Meanwhile, the pyridoxine in extracellular polymeric substances (EPS) and conductivity of anaerobic sludge from R3 increased more significantly than those of the others. Analysis of high-throughput sequencing indicated that the abundance of archaea increased in sludge from R3 and Methanosarcina responsible for DIET was dominant (63.64%). Additionally, the abundance of potential electroactive bacteria Chloroflexi in R3 was 7.57-fold, 3.61-fold and 7.37-fold as that of R1, R2 and R4, respectively. These results demonstrated that the electroactive microbes and methanogens could be enriched efficiently in anaerobic sludge via synergetic effect of magnetite addition and ethanol short-term stimulation.
- Published
- 2018
47. Visible-light-driven trimetallic Pt-Ag-Ni alloy nanoparticles for efficient nanoelectrocatalytic oxidation of alcohols
- Author
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Fei Gao, Yukihide Shiraishi, Pingping Song, Caiqin Wang, Yangping Zhang, Yukou Du, Jin Wang, Hui Xu, and Fangfang Ren
- Subjects
Materials science ,General Chemical Engineering ,Alloy ,Nanoparticle ,02 engineering and technology ,General Chemistry ,engineering.material ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Nanomaterial-based catalyst ,0104 chemical sciences ,chemistry.chemical_compound ,Adsorption ,chemistry ,Chemical engineering ,Alcohol oxidation ,Photocatalysis ,engineering ,0210 nano-technology ,Ethylene glycol ,Visible spectrum - Abstract
High-efficiency adsorption of solar energy is a lofty goal and the pursuit of efficient photocatalyst is also one of the tough challenges. It's a growing interesting in the technology that is on the basis of surface plasma resonance (SPR) plasma resonance of the alloy nanoparticles (NPs). We herein successfully design a facile class of trimetallic Pt-Ag-Ni alloy NPs that combined the advantages of electronic effect, size and synergistic effect. Impressively, the Pt2Ag1Ni1 NPs possess excellent electrocatalytic under visible light ethylene glycol oxidation reaction (EGOR) and glycerol oxidation reaction (GOR) with the mass activity founded to be: 7.99 and 4.88 A/mg, respectively. More significantly, SPR effect induces the optimal Pt-Ag-Ni NPs mass activity enhancement at 1.83 and 1.78 times higher under visible light than under environmental conditions. The design and SPR investigation of the trimetallic Pt-Ag-Ni alloy NPs in the research may provide a new approach for further development of high-performance nanocatalysts.
- Published
- 2018
48. Visible light enhanced electrochemical detection of caffeic acid with waxberry-like PtAuRu nanoparticles modified GCE
- Author
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Shumin Li, Yuting Shi, Yukou Du, Jin Wang, Caiqin Wang, Zhiping Xiong, Hui Xu, and Bo Yan
- Subjects
Materials science ,Nanocomposite ,Metals and Alloys ,Nanoparticle ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Electrochemistry ,01 natural sciences ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Catalysis ,Linear range ,Materials Chemistry ,Differential pulse voltammetry ,Electrical and Electronic Engineering ,Surface plasmon resonance ,0210 nano-technology ,Instrumentation ,Nuclear chemistry ,Visible spectrum - Abstract
In this work, trimetallic PtAuRu nanoparticles were synthesized by a facile one-pot method and glassy carbon electrode (GCE) modified with this trimetallic catalyst was used to detect caffeic acid (CA). Particularly, visible light illumination was introduced to assist the electrochemical assay and satisfactory results were achieved. The linear range obtained from differential pulse voltammetry (DPV) analysis was wide from 0.0087 mM to 16.6 mM. The obvious improvement of the response current towards CA is probably because of the surface plasmon resonance (SPR) effect of Au in the as-synthesized nanoparticles. These results indicate valuable potential of light illumination in enhancing the performance of metallic nanocomposites-based sensors in electrochemical detection.
- Published
- 2018
49. Phosphorus-doped cobalt-iron oxyhydroxide with untrafine nanosheet structure enable efficient oxygen evolution electrocatalysis
- Author
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Yukou Du, Lin Tian, Hui Xu, Caiqin Wang, Yangping Zhang, Jingjing Wei, and Chaofan Liu
- Subjects
Tafel equation ,Materials science ,Oxygen evolution ,chemistry.chemical_element ,02 engineering and technology ,Overpotential ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Electrocatalyst ,01 natural sciences ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Biomaterials ,Colloid and Surface Chemistry ,Chemical engineering ,chemistry ,Water splitting ,Chemical stability ,0210 nano-technology ,Cobalt ,Nanosheet - Abstract
Although explosive progresses have been achieved in the field of water splitting, the design and development of stable and inexpensive electrocatalysts for oxygen evolution remain a formidable challenge. Herein, the cost-efficient two dimensional (2D) phosphorus-doped CoFe oxyhydroxide nanosheets (denoted as CoFeP NSs) are successfully engineered and showing exceptional oxygen evolution reaction (OER) activity and chemical stability in 1 M KOH solution. This unique 2D nanosheet structure facilitates the mass transfer and electron transport, resulting in the remarkable OER activity that delivers a current density of 10 mA cm−2 at a low overpotential of 305 mV with an ultra-small Tafel slope 49.6 mV/dec. More significantly, the doped P also plays a vital role in modulating the surface active sites, leading to the substantial enhancement of electrocatalytic performances. Our study provides a facile one-pot method for the successful fabrication of 2D P-doped CoFe NSs which display superior electrocatalytic performance, shedding great promise for environment and energy-related fields.
- Published
- 2018
50. Additional file 5 of ALG3 contributes to stemness and radioresistance through regulating glycosylation of TGF-β receptor II in breast cancer
- Author
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Xiaoqing Sun, Zhenyu He, Guo, Ling, Caiqin Wang, Chuyong Lin, Liping Ye, Xiaoqing Wang, Li, Yue, Meisongzhu Yang, Sailan Liu, Hua, Xin, Wen, Wen, Lin, Chao, Zhiqing Long, Wenwen Zhang, Li, Han, Yunting Jian, Ziyuan Zhu, Xianqiu Wu, and Huanxin Lin
- Abstract
Additional file 5: Supplementary Figure 5. (A, B) Radioresistance-associated pathways were examined by luciferase assay to find out the most influenced pathway after ALG3-knocked out. Each bar represents the mean ± SD of three independent experiments. All data were analyzed by one-way ANOVA with Tukey’s multiple comparison test. (C) mRNA expression level of several genes that regulated by TGF-β pathway in ALG3-sg cells or cells treated by tunicamycin compared with ALG3 control cells were detected by RT-PCR. GAPDH was detected as a loading control in RT-PCR. (D) Upregulation of ALG3 decreased the apoptosis ratio, whereas inhibition of TGFBR2 increased the apoptosis ratio of ALG3-transduced cells after radiation treatment, which were detected by flow cytometry. (E) Statistical graphs of apoptotic cells proportion in the vector, ALG3-vector +LY2109761(TGFBR2 inhibitor), ALG3-transduced, and ALG3-transduced +LY2109761 cells were plotted. Each bar represents the mean ± SD of three independent experiments. Data were analyzed by Student’s t-test. (F) Upregulation of ALG3 led to increasing the number of spheres, while inhibition of TGFBR2 decreased the number of spheres in ALG3-overexpressing cells, which were detected by secondary sphere assay. Each bar represents the mean ± SD of three independent experiments. Data were analyzed by Student’s t-test. (G) Upregulation of ALG3 led to increasing proportion of the CD44+CD24−subpopulation, whereas inhibition of TGFBR2 decreased proportion of the CD44+CD24− subpopulation in ALG3-transduced cells. Each bar represents the mean ± SD of three independent experiments. All data were analyzed by Student’s t-test. *P
- Published
- 2021
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