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8 results on '"Cai Sulan"'

5. 7 α and 7 β Hydroxylation of Dehydroepiandrosterone by Gibberella sp. and Absidia Coerulea Biotransformation.

Author

Song, Ming, Fu, Ruicheng, Cai, Sulan, Jiang, Xuliang, Wang, Fuju, Xu, Weizhuo, and Xu, Wei

Subjects
DEHYDROEPIANDROSTERONE, HYDROXYLATION
Abstract

The hydroxylation of dehydroepiandrosterone (DHEA) to 7α -hydroxy-5-androstene-17-one (7α-OH-DHEA) and 7β-hydroxy-5-androstene-17-one (7β-OH-DHEA) by Gibberella sp. CICC 2498 and Absidia coerulea CICC 41050 was investigated. The media ingredients were optimized. Single factors such as the DHEA concentration, culture time, medium volume, and inoculum rate were individually investigated to generate optimum biotransformation conditions. An orthogonal optimization process using a four-factor, three- level L9 (33) experiment was designed and performed. Finally, the maximum production of 7β-OH-DHEA from DHEA biotransformation by Absidia coerulea is 69.61%. This strategy would provide a possible way to enhance the 7β-OH-DHEA yield in the pharmaceutical industry. [ABSTRACT FROM AUTHOR]

Published
2023
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7. A sensitive and rapid immunoassay for Mycoplasma pneumoniae in children with pneumonia based on single-walled carbon nanotubes

Author

Jiang Zhuquan, Weizhuo Xu, Wei Xu, Mingming Tao, Shi Li, Chunsheng Zhang, Ying Tang, Ming Song, Cai Sulan, and Ying Zhang

Subjects
Male, 0301 basic medicine, Mycoplasma pneumoniae, Microbiological culture, Adolescent, 030106 microbiology, lcsh:Medicine, medicine.disease_cause, Sensitivity and Specificity, Article, Antibodies, Microbiology, Serology, 03 medical and health sciences, Predictive Value of Tests, Pneumonia, Mycoplasma, medicine, Humans, Child, Saliva, lcsh:Science, Pathogen, Immunoassay, Multidisciplinary, biology, Nanotubes, Carbon, business.industry, lcsh:R, Respiratory infection, medicine.disease, Pneumonia, Child, Preschool, Predictive value of tests, biology.protein, Female, lcsh:Q, Gold, Antibody, business
Abstract

Mycoplasma pneumoniae(MP) is a leading pathogen of respiratory infection, especially community-acquired pneumonia (CAP), in children worldwide. However, its diagnosis is frequently ineffective because bacterial culture and serology test are usually positive 1–2 weeks or more after the disease onset. To achieve a better detection efficiency, the single-walled carbon nanotubes(SWCNT) were coupled with the colloidal gold-monoclonal antibody immunochromatographic strips(CGIC). Interestingly, the SWCNT/CGIC assay allowed MP identification, with a detection limit of 1 × 102 copies/ml. Using referenced throat swabs of 97 MP and 40 non-MP cases, the assay yielded 72.2% sensitivity, 100.0% specificity, 100.0% positive predictive value (PPV), 59.7% negative predictive value (NPV). In summary, our assay was far more effective than any conventional methods for the diagnosis of acute MP. The ease of use, rapid and stability further enhance its feasibility for clinical use on-site.

Published
2017
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