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Feline chronic gingivostomatitis (FCGS) is a challenge for the veterinary practitioner since its etiology and treatments are still undefined. The present paper investigated the role of the feline immunodeficiency virus (FIV) in the severity of the FCGS. Oral mucosal biopsies obtained from 19 cats with FCGS were divided into two groups according to their FIV serology status. Later, the clinical lesion score was correlated with the histopathological grade of FCGS lesions and the degree of immunostaining in both groups. Most of the animals had significant histological changes; however, no correlation with FIV immunostaining intensity was observed. It was concluded that the presence of FIV infection or the animal’s seropositivity status does not seem to interfere with the severity of clinical signs nor the degree of histopathological changes when compared to the seronegative group. A gengivoestomatite crônica felina (FCGS) é um desafio para o veterinário, uma vez que a sua etiologia e tratamentos permanecem indefinidos. O presente trabalho investigou o papel do vírus da imunodeficiência felina (FIV) na gravidade do FCGS. Biópsias da mucosa oral de 19 gatos com FCGS foram divididas em dois grupos de acordo com o status sorológico de FIV. Mais tarde, o escore de lesão clínica foi correlacionado com o grau histopatológico das lesões FCGS e o grau de imunocoloração em ambos os grupos. A maioria dos animais apresentou alterações histológicas significativas, porém não foi observada correlação com a intensidade de imunocoloração para FIV. Concluiu-se que a presença de infecção por FIV ou o estado soropositivo dos animais não parece interferir com a gravidade dos sinais clínicos nem com o grau de alterações histopatológicas quando comparado ao grupo soronegativo.

A gengivo-estomatite crônica é uma patologia oral caracterizada por apresentar lesões inflamatórias crônicas que atinge principalmente o arco glossopalatino. A sua etiologia ainda é desconhecida, mas parece ser multifatorial e apresenta fatores imunomediados. O diagnóstico deve ser baseado na anamnese, exame clínico e análises laboratoriais, devendo a terapêutica ser instituída de acordo com o caso clínico. Este trabalho tem por objetivo apresentar breve revisão de literatura relativa à patogenia, sinais clínicos, diagnóstico e tratamento da gengivo-estomatite crônica felina. Além disso, pretendemos alertar aos médicos veterinários quanto à importância da enfermidade e apresentar as dificuldades diagnósticas e terapêuticas de acordo com o estágio de evolução da doença.

Made available in DSpace on 2015-10-06T13:02:50Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-12-12. Added 1 bitstream(s) on 2015-10-06T13:19:26Z : No. of bitstreams: 1 000847194.pdf: 1197011 bytes, checksum: 7209fde66f8374f6f0f630a8a112ded9 (MD5) Herpesvirus bovino (BoHV-5) é um alfaherpesvirus que causa meningoencefalite não-supurativa preferencialmente em bovinos jovens. Esta enfermidade ocorre naturalmente em surtos ou casos isolados, apresentando baixa morbidade e alta letalidade. A epidemiologia, as características anatomopatológicas e o diagnóstico do BoHV-5 são bem conhecido. No entanto, as interações moleculares entre a células hospedeiras e o BoHV-5 são pobremente compreendidas. Técnicas moleculares como a PCR quantitativa e o sequenciamento de RNA (RNA-seq) são importantes ferramentas para o estudo de interações entre vírus e células hospedeiras. Neste estudo células MDBK foram infectadas pelo BoHV-5, cepa SV507-99 e o mRNA extraído em 0, 6, 12, 18, e 24 horas após a infecção (pi) foi utilizado para avaliar a expressão de genes do vírus e da célula hospedeira por qPCR e o mRNA de 1h, 6h e 24h pi foram utilizado no estudo por RNA-seq. Células MDBK não infectadas foram usadas como controle. A qPCR demonstrou que os três genes do BoHV-5 estudados (bICP0, UL9 e US4) apresentaram perfil de expressão semelhante nos diferentes momentos após infecção e que o gene bovino GAPDH teve sua expressão diminuída pela infecção viral. Ao menos 900 genes foram diferencialmente expressos para cada momento de infecção na análise por RNA-seq. Estes genes estavam principalmente relacionados a vias celulares de controle de ciclo celular, produção de interleucinas, quimiotaxia para células inflamatórias, reparo e dano ao DNA, resposta a vírus, apoptose, processo oxidativo, e ubiquitização de moléculas. Estes resultados representam novos conhecimentos sobre a interação entre o BoHV-5 e as células bovinas e podem representar um ponto de partida para novas pesquisas e melhor compreensão da patogenia deste vírus Bovine herpesvirus 5 (BoHV-5) is an Alphaherpesvirus that causes nonsuppurative meningoencephalitis mainly in young cattle. This disease occurs naturally in either outbreaks or isolated cases, and exhibits low morbidity and high lethality. Besides BoHV-5 epidemiology, pathological findings and diagnosis were well known, the molecular interactions between host cell and BoHV-5 are poorly understood. Molecular biology techniques such as quantitative PCR (qPCR) and RNA sequencing (RNA-seq) are important tools to study virus and host cell interactions. In this study we infected MDBK cells and use the extracted mRNA at 0, 6, 12, 18 and 24h post infection (pi) to analyse BoHV-5 (bICP0, UL9 e US4) and host cell gene (GAPDH) expression using qPCR and 1h, 6h and 24h pi in the RNA-seq study. Mock-infected cells were used to control purpouse. The qPCR releveled that the three BoHV-5 genes showed the same expression behavior during the infection and the GAPDH gene were down-regulated in the infected group. At least 900 genes were differentially expressed during BoHV-5 in each moment analysed by RNA-seq. These genes were up- or down-regulated and mainly associated with cell cycle, interleukin production, inflammatory cells chemotaxis, DNA damage and repair, response to virus, apoptosis, oxidation-reduction process, and ubiquitination pathways. The results demonstrate new insights about BoHV-5 and bovine cells interactions and could be a starting point to new researches and to better understand the pathology of this virus

Made available in DSpace on 2020-12-10T18:04:15Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-01-01 Background: Choroid plexus tumors are uncommon neoplasms derived from the neuroepithelium that covers the ventricular cavity and the central canal of the spinal cord that are characterized by papillar aspect and intraventricular growth. It occurs more commonly in dogs, but it has been reported in horses, cows, ferrets and is rare in cats. After the meningiomas and gliomas, choroid plexus tumors are the third encephalic tumors in dogs. In veterinary medicine, there are not many actual reports of this type of neoplasia, therefore, the aim of this paper was to report a case of a choroid plexus papilloma in a poodle dog. Case: A six year old dog, female, poodle, was admitted at the Veterinary Hospital of the Sao Paulo State University 'Julio de Mesquita Filho' with a complaint of seizures for 15 days, blindness and head tilt to the right side. At the neurological examination, it had depression, head tilt to the right, circling and ataxia, postural reactions deficits, absent menace response, absent light pupillary reflex with mydriasis and augmented patellar reflex. The patient was submitted to blood harvest to perform CBC count and biochemical profile (renal and hepatic), which were normal. The cerebrospinal fluid analysis presented a discrete raise of proteins. The animal was treated with phenobarbital and prednisone, under suspicion of inflammatory disease or encephalic neoplasia. The patient developed a progressive paresis of the four limbs and the seizures remained unaltered. After approximately one month of the first consultation, the dog was admitted in status epileticus, without success of control. Necropsy revealed flattening of convolutions due to cerebral edema, dilatation of the lateral ventricles (hydrocephalus) and the presence of a greyish nodule, soft and measuring 0.6 x 0.8 cm in length coming out of the fourth ventricle and extending laterally between the cerebellum and brainstem and cranially to the thalamus. At the cut of the tissue, it presented a dark area at the region of the thalamus. Neoplastic tissue was composed of cuboidal to columnar epithelial cells with oval and regular nuclei, supported by richly vascular stroma, arranged in papillae. Discussion: Choroid plexus papillomas are tumors that originate in the choroid plexus epithelium. Affected dogs usually have six or more years, earlier than most brain tumors. However, cases have been observed in younger dogs, and dogs affected by carcinoma are generally older than those affected by the papilloma. In dogs, the tumor occurs most commonly in the fourth ventricle, as in the present report, but can also occur in the third ventricle and the lateral ventricles. The clinical signs in patients with choroid plexus tumors are varied depending on the location of the tumor in the central nervous system, but may include behavioral changes, ataxia, paresis, seizures, circling, cranial nerve and proprioceptive reflexes changes. Macroscopically, the choroid plexus papilloma is a well-defined mass, expansive, granular to papillary, grayish-white to red color. Microscopically, this tumor resembles the choroid plexus and have arboriform and vascular stroma which is coated by a single layer of cuboidal to columnar epithelial cells. The findings were consistent with choroid plexus papilloma located in the fourth ventricle. UNESP, FMVZ, Univ Estadual Paulista Julio de Mesquita Filho, Programa Posgrad Med Vet, BR-18618970 Botucatu, SP, Brazil MV Autonoma, Bauru, SP, Brazil UNESP, FMVZ, Dept Clin Vet, BR-18618970 Botucatu, SP, Brazil UNESP, FMVZ, Univ Estadual Paulista Julio de Mesquita Filho, Programa Posgrad Med Vet, BR-18618970 Botucatu, SP, Brazil UNESP, FMVZ, Dept Clin Vet, BR-18618970 Botucatu, SP, Brazil

Made available in DSpace on 2016-04-01T18:43:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2013. Added 1 bitstream(s) on 2016-04-01T18:49:29Z : No. of bitstreams: 1 ISSN1679-9216-2013-41-13-01-07.pdf: 194429 bytes, checksum: ed08ef2b25d531745e7827294e23f005 (MD5) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Background: Choroid plexus tumors are uncommon neoplasms derived from the neuroepithelium that covers the ventricular cavity and the central canal of the spinal cord that are characterized by papillar aspect and intraventricular growth. It occurs more commonly in dogs, but it has been reported in horses, cows, ferrets and is rare in cats. After the meningiomas and gliomas, choroid plexus tumors are the third encephalic tumors in dogs. In veterinary medicine, there are not many actual reports of this type of neoplasia, therefore, the aim of this paper was to report a case of a choroid plexus papilloma in a poodle dog. Case: A six year old dog, female, poodle, was admitted at the Veterinary Hospital of the São Paulo State University ‘Júlio de Mesquita Filho’ with a complaint of seizures for 15 days, blindness and head tilt to the right side. At the neurological examination, it had depression, head tilt to the right, circling and ataxia, postural reactions defi cits, absent menace response, absent light pupillary refl ex with mydriasis and augmented patellar refl ex. The patient was submitted to blood harvest to perform CBC count and biochemical profi le (renal and hepatic), which were normal. The cerebrospinal fl uid analysis presented a discrete raise of proteins. The animal was treated with phenobarbital and prednisone, under suspicion of infl ammatory disease or encephalic neoplasia. The patient developed a progressive paresis of the four limbs and the seizures remained unaltered. After approximately one month of the fi rst consultation, the dog was admitted in status epileticus, without success of control. Necropsy revealed fl attening of convolutions due to cerebral edema, dilatation of the lateral ventricles (hydrocephalus) and the presence of a greyish nodule, soft and measuring 0.6 x 0.8 cm in length coming out of the fourth ventricle and extending laterally between the cerebellum and brainstem and cranially to the thalamus. At the cut of the tissue, it presented a dark area at the region of the thalamus. Neoplastic tissue was composed of cuboidal to columnar epithelial cells with oval and regular nuclei, supported by richly vascular stroma, arranged in papillae. Discussion: Choroid plexus papillomas are tumors that originate in the choroid plexus epithelium. Affected dogs usually have six or more years, earlier than most brain tumors. However, cases have been observed in younger dogs, and dogs affected by carcinoma are generally older than those affected by the papilloma. In dogs, the tumor occurs most commonly in the fourth ventricle, as in the present report, but can also occur in the third ventricle and the lateral ventricles. The clinical signs in patients with choroid plexus tumors are varied depending on the location of the tumor in the central nervous system, but may include behavioral changes, ataxia, paresis, seizures, circling, cranial nerve and proprioceptive refl exes changes. Macroscopically, the choroid plexus papilloma is a well-defi ned mass, expansive, granular to papillary, grayishwhite to red color. Microscopically, this tumor resembles the choroid plexus and have arboriform and vascular stroma which is coated by a single layer of cuboidal to columnar epithelial cells. The fi ndings were consistent with choroid plexus papilloma located in the fourth ventricle. Universidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Clínica Veterinária, Faculdade de Medicina Veterinária e Zootecnia de Botucatu, Botucatu, Rubião Júnior s/n, CEP 18618-000, SP, Brasil Universidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Clínica Veterinária, Faculdade de Medicina Veterinária e Zootecnia de Botucatu, Botucatu, Rubião Júnior s/n, CEP 18618-000, SP, Brasil

Made available in DSpace on 2016-07-07T12:34:05Z (GMT). No. of bitstreams: 0 Previous issue date: 2012. Added 1 bitstream(s) on 2016-07-07T12:43:18Z : No. of bitstreams: 1 ISSN2178-3764-2012-19-02-186-191.pdf: 773932 bytes, checksum: 2ddd4f6c3404e844fb794e690655b07b (MD5) A esporotricose é uma micose de humanos e animais, causada por um fungo dimórfico chamado Sporothrix schenckii. Os animais domésticos, particularmente os gatos, são importantes fontes de infecção para humanos. O diagnóstico da esporotricose pode ser realizado com o uso de exames citológicos e histopatológicos, isolamento fúngico, imunofluorescencia ou por métodos moleculares. O presente trabalho relata o diagnóstico citológico com agulha fina e o tratamento de esporotricose em um felino. Sporotrichosis is a mycotic disease of humans and animals caused by a dimorphic fungus called Sporothrix schenckii. Domestic animals, particularly cats, play an important role for human infections. The diagnosis of sporotrichosis can be made by using cytologic and histopathologic examination, culturing the fungus, immunofluorescence or molecular methods. This paper describes the cytologic diagnosis by fine-needle aspiration and treatment of a case of feline sporotrichosis. La esporotricosis es una enfermedad micótica que afecta a los humanos y a los animales provocada por el hongo dimórfico Sporothrix schenckii. La infección es usualmente provocada por la inoculación traumática con tierra, plantas y materia orgánica contaminada por el hongo. Los animales domésticos, en particular los gatos, son fuentes importantes de transmisión para el hombre. El diagnóstico de esporotricosis puede ser realizado mediante el examen citológico y histopatológico, aislamiento del hongo, inmunofluorescencia y métodos moleculares. En este trabajo, se reporta el diagnóstico por medio de citología por aspiración con aguja fina así como el tratamiento de un caso de la esporotricosis felina. Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Departamento de Clínica Veterinária, Botucatu, SP, Brasil Pontifícia Universidade Católica de Minas Gerais (PUC Minas), Poços de Caldas, MG, Brasil Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Departamento de Clínica Veterinária, Botucatu, SP, Brasil

Made available in DSpace on 2014-06-11T19:23:47Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-02-15Bitstream added on 2014-06-13T20:51:06Z : No. of bitstreams: 1 cagnini_dq_me_botfmvz.pdf: 1790427 bytes, checksum: 7e7453715dc898af589170842b6953fe (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) A meningoencefalite herpética bovina é uma infecção viral do SNC causada pelo BoHV-5, um vírus DNA de fita dupla, envelopado, pertencente á família Herpesviridae, subfamília Alphaherpesvirinae. A meningoencefalite acomete principalmente animais jovens, ocorrendo principalmente em forma de surtos de casos isolados ou de surtos com morbidade baixa e letalidade é alta. A doença não possui caráter sazonal e nem predileção por raça ou sexo. Surtos de meningoencefalite por BHV-5 têm sido relatados após algumas práticas de manejo, todas associadas a situações de estresse. O diagnóstico final baseiase nos achados clínicos, epidemiológicos, macro e microscópicos e o isolamento viral associado a técnicas como imunoperoxidase, restrição da endonuclease e PCR. Entrentanto, o isolamento viral nem sempre é possível devido a distância das fazendas e ao tempo entre a morte dos animais e a coleta das amostras. Nestes casos a imunoistoquímica e a PCR são ferramentas diagnósticas úteis. Além disso, estas técnicas permitem o diagnóstico a partir de tecido fixado em formol e incluído em parafina. O objetivo deste trabalho foi descrever as alterações histopatológicas nas diferentes regiões do sistema nervoso central de bovinos infectados experimentalmente pelo BoHV-5 e relacionar a intensidade e o tipo das lesões encontradas com os resultados da PCR e da imunoistoquímica (IHQ) para detecção do BoHV-5. Para isso, blocos de parafina do sistema nervoso central de seis bovinos provenientes de animais inoculados experimentalmente pelo BoHV-5 foram avaliados microscopicamente e pelas técnicas da IHQ e da PCR. Todos os animais apresentaram alterações histopatológicas de meningoencefalite não supurativa, com intensidade variável entre as áreas e os animais, sendo que as regiões do córtex cerebral apresentaram alterações microscópicas mais acentuadas... Bovine meningoencephalitis is a viral infection caused by BHV-5, a doublestranded DNA and enveloped virus, that belongs to the Herpesviridae family, subfamily Alphaherpesvirinae. Meningoencephalitis mainly occurs in young animals, causing individual deaths or outbreaks with low morbidity and high mortality. The disease has no seasonal character or predilection for breed or gender. Outbreaks of meningoencephalitis have been reported associated with stressful factors like weaning, vaccinations, transport, changes in feeding. The final diagnosis is based on clinical, epidemiological, gross and microscopic findings and virus isolation associated with others techniques like immunoperoxidase, endonucleases restriction and PCR. Wherever, virus isolation is no always possible due the distance of farms and time from animal death to the samples collection. Immunohistochemistry and PCR are better diagnostic tools in this cases. Besides this two techniques could be used in formalin-fixed, paraffin-embedded tissues. The aim of this study was to describe the histological changes in the CNS of BHV-5 experimentally infected calves and associate it with the PCR and IHC results. For this purpose, formalin-fixed, paraffin-embedded CNS tissue from previously BHV-5 inoculated calves (Cunha, 2010) were microscopically evaluated and were tested by immunohistochemistry (IHC) and polymerase chain reaction (PCR). All animals presented non-suppurative meningoencephalitis that had variable distribution among the cases and among the various sections of brain regions in each case. The cerebral cortex had more accentuated lesions. From 108 evaluated areas, 32.41% and 35.19% were positives by IHC and PCR, respectively. The IHQ and PCR positive areas had more accentuated microscopic lesions. These findings extend the knowledge about the BHV-5 distribution in the CNS of calves and show that IHC and PCR could be useful

Made available in DSpace on 2016-07-07T12:34:08Z (GMT). No. of bitstreams: 0 Previous issue date: 2011. Added 1 bitstream(s) on 2016-07-07T12:43:23Z : No. of bitstreams: 1 ISSN0102-5716-2011-18-02-313-319.pdf: 187636 bytes, checksum: aad8d8cb3800592a77e46714e5efa3cc (MD5) Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP) O presente trabalho objetivou estabelecer a concentração do sulfeto de hidrogênio ruminal utilizando tubos colorimétricos em bovinos Nelore sadios criados em pasto de Brachiaria decumbens contendo 0,15% de enxofre na matéria seca. Quatro bovinos foram submetidos a exames físicos e laboratoriais, com intervalos de 96 horas, durante período de 40 dias. Os resultados indicaram que a técnica se mostrou adequada e que as dosagens de sulfeto de hidrogênio ruminal foram inferiores a 100 ppm. A técnica utilizando tubos colorimétricos para dosagem de sulfeto de hidrogênio ruminal foi de fácil execução a campo e os bovinos ingerindo B. decumbens com 0,15% de enxofre na matéria seca apresentaram concentração mínima de 25 ppm e máxima de 100 ppm de gás sulfídrico ruminal. The aim of this work was to establish the concentration of ruminal hydrogen sulfide using a detector tube method in health Nelore bovine kept in Brachiaria decumbens pasture (0.15% S dry matter). Four animals were clinically evaluated and laboratorial exams performed with 96 hour interval, during 40 days of experimental period. Clinical and laboratorial results indicated that technique was suitable and ruminal sulfide hydrogen values were inferior to 100 ppm. It was concluded that the technique was simple and healthy Nelore bovine kept in Brachiaria decumbens pasture with sulfur content of 0.15% of dry matter presented minimal concentrations of 25 ppm and maximum of 100 ppm of H2S. La ingestión de dietas con altas concentraciones de azufre pueden ocasionar la producción excesiva de sulfuro de hidrógeno ruminal (H2S), pudiendo ocasionar polioencefalomalacia en vacunos. El presente estudio tuvo como objetivo determinar la concentración de sulfuro de hidrógeno en el rumen utilizando tubos colorimétricos en vacunos Nelore, sanos, creados en pastura de Brachiaria decumbens, conteniendo 0,15% de azufre en la materia seca. Fueron realizados exámenes clínicos y laboratoriales en cuatro vacunos, a intervalos de 96 horas, durante un período de 40 días. Los resultados indicaron que la técnica era conveniente y que el dosaje de sulfuro de hidrógeno ruminal en los mismos fue inferior a 100 ppm. La técnica de utilización de tubos colorimétricos para el dosaje de sulfuro de hidrógeno ruminal a campo fue de ejecución fácil y como resultado los bovinos tuvieron una concentración mínima de 25 ppm e máxima de 100 ppm de gas sulfhídrico ruminal. Universidade Federal de Goiás (UFG), Escola de Veterinária, Departamento de Medicina Veterinária, Goiânia, GO, Brasil Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Departamento de Clínica Veterinária, Botucatu, SP, Brasil Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Departamento de Clínica Veterinária, Botucatu, SP, Brasil FAPESP: 2006/05836-6

Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:25:23Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:41:35Z : No. of bitstreams: 1 2-s2.0-79953117390.pdf: 2295290 bytes, checksum: 8479b8ac9c07f4879ce4848b4ac202cd (MD5) Made available in DSpace on 2014-05-27T11:25:23Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-12-01 The present study reports the occurrence of lead poisoning in a male, 2 year-old mongrel dog attended at UNESP Botucatu Veterinary Hospital. The animal was brought to the hospital with claim of gastrointestinal and nervous disturbs for 10 days, worsening in the last 24 hours. After unsuccessfully attempts to control status epileticus, the animal died. Hematological evaluation revealed significant rubricytosis and basophilic stippling, highly suggestive of lead poisoning. Serum lead concentration confirmed toxic levels of this element. Necropsy evidenced the presence of foreign bodies in stomach (one stone and two metallic objects) and, microscopically, characteristic inclusions in renal parenchyma. Fragments obtained from liver and kidneys revealed lead concentrations of 0, 8 μg/g and 25 μg/g, respectively, which in association to clinical signs of saturnism, determined the diagnosis of lead poisoning. Universidade Estadual Paulista (UNESP), Campus de Botucatu Universidade Estadual Paulista (UNESP), Campus de Botucatu

Atualmente, tem-se estudado a predisposição de cadelas obesas ao desenvolvimento tumoral. Em mulheres, sabe-se que a obesidade pode estar associada ao aparecimento de tumores de mama. Nos animais de companhiaesse dado ainda é escasso, principalmente associando a clínica da paciente com achados laboratoriais e histológicos. Este trabalho objetivou avaliar os aspectos clínicos e laboratoriais de cadelas com neoplasia mamária, investigando se possuem relação com a obesidade. Foram estudados quatro grupos de cadelas, castradas ou inteiras, divididos em: (G1) cadelas sem tumor de mama e escore de condição corporal normal (ECC), (G2) cadelas sem tumor de mama e obesas, (G3) cadelas com tumor de mama e ECC normal, (G4) cadelas com tumor de mama e obesas. Foram avaliados o Escore de Condição Corporal (ECC), Índice de Massa Corporal Canina (IMCC) hemograma, glicose, colesterol total, triglicerídeos, exame citopatológico e histopatológico. Os animais sem raça definida foram os mais presentes no estudo (67,5%). Na análise do Índice de Massa Corporal Canina ocorreu diferença estatística entre grupo G2 e G4. A glicose também diferiu estatisticamente entre os grupos G1xG3 e G2xG4. Portanto, observou-se que a obesidade não desencadeou alterações clínicas e laboratoriais nas cadelas. Não foi observada relação entre o ECC e do IMCC com o tipo histológico ou malignidade da neoplasia mamária, pois tanto no grupo com escore ideal quanto no grupo com sobrepeso a obeso os tipos histológicos malignos foram mais presenciados. Houve uma limitação quanto ao uso do índice de massa corporal canino em relação a proporcionalidade corpórea. Dos 28 tumores avaliados, o carcinoma em tumor misto foi o tipo histológico maligno mais presente e de benigno foi o adenomioepitelioma. Presently, the predisposition of obese bitches to tumor development has been studied. In women, it is known that obesity may be associated with the appearance of breast tumors. In companion animals this data is still scarce, mainly associating the clinic of the patient with laboratory and histological findings. This study aimed to evaluate the clinical and laboratory aspects of bitches with breast neoplasia, investigating whether they are related to obesity. Four groups of bitches, either castrated or whole, were divided into: (G1) bitches without breast tumor and normal body condition score (GCE), (G2) bitches without breast tumor and obese, (G3) bitches with tumor of breast and normal ECC, (G4) bitches with breast and obese tumors. The Body Condition Score (ECC), Canine Body Mass Index (IMCC), blood glucose, total cholesterol, triglycerides, cytopathological and histopathological examination were evaluated. The non-defined animals were the most present in the study (67.5%). In the analysis of the Canine Body Mass Index there was a statistical difference between group G2 and G4. Glucose also differed statistically between the G1xG3 and G2xG4 groups. Therefore, it was observed that obesity did not trigger clinical and laboratory changes in bitches. No relationship was found between ECC and the IMCC with the histological type or malignancy of the mammary neoplasia, since both the ideal and the overweight group had obese malignant histological types. There was a limitation on the use of canine body mass index in relation to body proportionality. Of the 28 tumors evaluated, carcinoma in mixed tumor was the most present malignant histological type and benign was adenomioepithelioma.

Embora o uso de cartilagem elástica de origem animal, como xenoenxerto, seja conhecido na rotina veterinária, suas propriedades e as reações que provocam no organismo do animal ainda são pouco conhecidas, principalmente quando tratadas em solução alcalina. O conhecimento dessas particularidades poderá possibilitar maior segurança no desenvolvimento de novos biomateriais para a rotina clínica e cirúrgica humana e veterinária. Desta forma, este estudo teve como objetivo principal analisar comparativamente a biocompatibilidade entre as cartilagens elásticas bovinas tratada em solução alcalina e as cartilagens não tratadas, implantadas em ratos Wistar. As cartilagens foram obtidas de orelhas bovinas e, metade destas foram tratadas em solução alcalina e a outra metade não foram tratadas. Independente do tratamento, as cartilagens foram esterilizadas em óxido de etileno. As cartilagens foram implantadas no subcutâneo da região dorsal, entre as escápulas em ratos (N=50) sendo que GE (N=25) receberam cartilagens tratadas e GC (N=25) receberam cartilagens não tratadas. Para a retiradas dos implantes foram eutanasiados cinco animais de cada grupo nos dias 3, 7, 14, 21 e 42 sendo que as características macroscópicas desencadeadas pelo implante foram avaliadas e estadiadas como fibroses, coágulos e infecções. Foram realizadas avaliações histopatológicas para verificar a reação tecidual, qualificando e quantificando-se o processo inflamatório e da proliferação do tecido conjuntivo. Os resultados mostram que GE possui menor quantidade de infiltrado inflamatório e melhor organização do tecido conjuntivo em relação a GC. Os padrões de degradação da cartilagem em GE mostraram diminuição na matriz cartilaginosa em relação a GC e não houve diferença significativa do processo de neovascularização entre os grupos. Concluiu-se que o tratamento alcalino proporcionou melhor biocompatibilidade para a cartilagem elástica quando implantada no subcutâneo de ratos. Although the use of elastic cartilage of animal origin, as xenograft, is known in the veterinary routine, its properties and the reactions that provoke in the organism of the animal are still little known, especially when treated in alkaline solution. Knowledge of these particularities may allow greater safety in the development of new biomaterials for routine human and veterinary clinical and surgical procedures. Thus, the main objective of this study was to analyze the biocompatibility between bovine elastic cartilage treated with alkaline solution and untreated cartilage implanted in Wistar rats. Cartilages were obtained from bovine ears and half of them were treated in alkaline solution and the other half were not treated. Regardless of the treatment, the cartilages were sterilized in ethylene oxide. Cartilages were implanted in the subcutaneous region of the dorsal region, between the scapulae in rats (N = 50), and SG (N = 25) received treated cartilages and CG (N = 25) received untreated cartilages. Five animals from each group were euthanized on days 3, 7, 14, 21 and 42 and the macroscopic characteristics triggered by the implant were evaluated and staged as fibroses, clots and infections. Histopathological evaluations were performed to verify the tissue reaction, qualifying and quantifying the inflammatory process. The results show that SG has a smaller amount of inflammatory infiltrate and better organization of the connective tissue in relation to CG. Cartilage degradation patterns in SG showed a decrease in the cartilage matrix in relation to CG and there was no significant difference in the neovascularization process between the groups. It was concluded that the alkaline treatment provided better biocompatibility for the elastic cartilage when implanted in the subcutaneous of rats. Fundação de Amparo à Pesquisa do Estado de Goiás - FAPEG