1. The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A
- Author
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Jonas Seiler, Annika Eiteneuer, Hemmo Meyer, Monica Gotta, René Holtackers, Françoise Schwager, Esther Zanin, François Prodon, Asako Sugimoto, Patrick Meraldi, Elsa Kress, and Mika Toya
- Subjects
Cell division ,Medizin ,Caenorhabditis elegans Proteins/metabolism/physiology ,Mitosis ,Centrosome cycle ,Cell Cycle Proteins ,Biology ,Protein Serine-Threonine Kinases ,Adaptor Proteins, Signal Transducing/metabolism/physiology ,Article ,Gene Expression Regulation, Enzymologic ,Cell Line ,03 medical and health sciences ,0302 clinical medicine ,Animals ,Humans ,Caenorhabditis elegans Proteins ,ddc:612 ,Research Articles ,030304 developmental biology ,Adaptor Proteins, Signal Transducing ,Green Fluorescent Proteins/metabolism ,Adenosine Triphosphatases ,Centrosome ,0303 health sciences ,Cell Cycle Proteins/metabolism ,Cell Cycle ,Gene Expression Regulation, Developmental ,Cell Biology ,Adenosine Triphosphatases/metabolism ,Protein-Serine-Threonine Kinases/metabolism ,Cell cycle ,Caenorhabditis elegans/cytology/genetics ,Cell biology ,Spindle apparatus ,Spindle checkpoint ,Centrosome/metabolism ,RNA Interference ,Centrosome separation ,030217 neurology & neurosurgery - Abstract
UBXN-2, a substrate adaptor of the AAA ATPase CDC-48/p97, is required to coordinate centrosome maturation timing with mitosis., Coordination of cell cycle events in space and time is crucial to achieve a successful cell division. Here, we demonstrate that UBXN-2, a substrate adaptor of the AAA ATPase Cdc48/p97, is required to coordinate centrosome maturation timing with mitosis. In UBXN-2–depleted Caenorhabditis elegans embryos, centrosomes recruited more AIR-1 (Aurora A), matured precociously, and alignment of the mitotic spindle with the axis of polarity was impaired. UBXN-2 and CDC-48 coimmunoprecipitated with AIR-1 and the spindle alignment defect was partially rescued by co-depleting AIR-1, indicating that UBXN-2 controls these processes via AIR-1. Similarly, depletion in human cells of the UBXN-2 orthologues p37/p47 resulted in an accumulation of Aurora A at centrosomes and a delay in centrosome separation. The latter defect was also rescued by inhibiting Aurora A. We therefore postulate that the role of this adaptor in cell cycle regulation is conserved.
- Published
- 2013