Search

The present study was conducted to investigate the heavy metal pollution status of the agricultural soils in Ramhormoz Plain, Khuzestan province, Iran. A total of 54 samples from the depths (0–20 cm) of the soil were collected from the selected areas in (March) 2021. According to the obtained results, the concentrations of As, Cd, Cr, Cu, Hg, Mn, Ni, Pb, Zn, Al in soil samples were 1.3, 0.68, 60, 10.6, 0.01, 218, 23, 3.65, 14 and 0.58 mg/kg, respectively, in which As showed significant soil contamination and Cu, Hg, Mn, Ni, Pb, Zn and Al moderate contamination according to the calculation of contamination factor (CF). PCA analysis revealed that both anthropogenic and natural sources of pollution like agricultural and transport activities contributed to the enrichment of study area. Investigation of ecological risk indicated that Ramhormoz Plain was categorized as a high risk area. [ABSTRACT FROM AUTHOR]

The works of literature evaluating the eco-environmental risks posed by metals in agricultural areas in developing countries remains limited. This study sought to evaluate the environmental and ecological risks posed by metals in the intensively cultivated areas of the Amik Plain as well as to determine the origins of the metals. For this purpose, 137 soil samples were taken from agricultural production areas of the Amik Plain, and 11 metals (Al, Fe, Ni, Pb, Co, Cr, Cu, Mn, Zn, Cd and As) were examined in the samples. As Ni had the highest average enrichment factor (EF) value (8.04) when compared with the other metals, the soils were found to be significantly enriched with Ni. The Pearson correlation analysis and principal component analysis showed that the Zn concentration was controlled by lithogenic sources, while the Ni, Pb, Cd, Cr and Cu concentrations were controlled by both anthropogenic and lithogenic sources. [ABSTRACT FROM AUTHOR]

Background: Despite the undisputed benefits of breastfeeding, infants might become exposed to xenobiotics that could be excreted into breast milk following maternal exposure. This study was conducted to assess breastmilk cadmium levels among lactating women in Palestine, a resource-limited country. Estimated daily intake (EDI) of cadmium via breastmilk was also calculated and predictors of high breastmilk cadmium levels and high infant exposure via breastmilk were identified. Methods: This multicenter study was conducted using a descriptive-analytical design. The lactating women were recruited from different maternity and public health clinics in all regions of Palestine. Demographic variables and exposure to sources of cadmium were collected in an interviewer-administered questionnaire. Foremilk samples (about 5 mL) were collected in polyethylene tubes using the hand-expression technique. The breast milk samples were collected in the period between December 2020 and March 2021. A pre-validated method using inductively coupled plasma mass spectrometry (ICP-MS) was used to quantify breastmilk cadmium levels. EDI values were calculated from the quantified breastmilk cadmium levels. Results: Breastmilk samples were obtained from 256 lactating women. The mean breastmilk cadmium level was 0.34 (SD: 0.33) μg / L and the mean EDI of cadmium via breastmilk was 0.059 (SD: 0.058) µg / kg per body weight / day. Breastmilk cadmium levels were quantified in 92.6% of the breastmilk samples. Of the breastmilk samples, 13 (5.1%) had cadmium levels above those reported as "normal" by the World Health Organization (WHO). Multiple linear regression showed that higher breastmilk cadmium levels and higher EDI were predicted by being a smoker, living in a refugee camp, living close to an industrial area, living close to disposal of wastes, living close to paint shops, living in a house with peeling / chipping paint, frequent use of cosmetics, frequent use of hair dyes, and not using vitamins. Conclusion: The breastmilk cadmium levels and infant exposure were predicted by maternal exposure to sources of cadmium. The findings reported in this study are valuable to antenatal and postnatal healthcare service providers. More studies are needed to plan and implement measures to reduce breastmilk cadmium levels and infants' exposure to cadmium via breastmilk. [ABSTRACT FROM AUTHOR]

cadmium usually enter the environment and water resources through wastewater, released by various industries, and may have adverse effects. The current study employs surface of bentonite clay available locally in order to remove cadmium In solutions contaminated with this type of ions, in order to research on a surface with a high ability to adsorption of cadmium (II) ions, study Some factors affect the adsorption process on bentonite clay, such as contact time, pH the solution, Adsorbent particle size, Initial concentration of solutions and temperature of the solution were examined in the a batch process mode. The amount of adsorbed Cd (II) increased with height temperature, the optimum adsorption pH was about 6.5. Under this condition, the percent removal was 95.17%. The adsorption isotherms were studied and the results of adsorption processes were more fitted with Friendlich model rather than Langmuir adsorption model. Thermodynamic study showed that, ΔH was endothermic, ΔG is found to be negative That is, the process is automatic and ΔS was found to be positive. The current study also involves practical application using bentonite to get rid of Cd(II) ions to from wastewater of Hamdan's station of the Basra-iraq, The results indicate high affinity (97.84%) removal of Cd(II) ions. [ABSTRACT FROM AUTHOR]

BACKGROUND: Elevating dietary calcium (Ca) intake can reduce metal(loid)oral bioavailability. However, the ability of a range of Ca minerals to reduce oral bioavailability of lead (Pb), cadmium (Cd), and arsenic (As) from indoor dust remains unclear. OBJECTIVES: This study evaluated the ability of Ca minerals to reduce Pb, Cd, and As oral bioavailability from indoor dust and associated mechanisms. METHODS: A mouse bioassay was conducted to assess Pb, Cd, and As relative bioavailability (RBA) in three indoor dust samples, which were amended into mouse chow without and with addition of CaHPO4, CaCO3, Ca gluconate, Ca lactate, Ca aspartate, and Ca citrate at 200–5,000 μg/g Ca. The mRNA expression of Ca and phosphate (P) transporters involved in transcellular Pb, Cd and As transport in the duodenum of mice was quantified using real-time polymerase chain reaction. Serum 1,25-Dihydroxyvitamin D3 [1,25(OH)2D3], parathyroid hormone (PTH), and renal CYP27B1 activity controlling 1,25(OH)2D3 synthesis were measured using ELISA kits. Metal(loid) speciation in the feces of mice was characterized using X-ray absorption near-edge structure (XANES) spectroscopy. RESULTS: In general, mice exposed to each of the Ca minerals exhibited lower Pb-, Cd-, and As-RBA for three dusts. However, RBAs with the different Ca minerals varied. Among minerals, mice fed dietary CaHPO4 did not exhibit lower duodenal mRNA expression of Ca transporters but did have the lowest Pb and Cd oral bioavailability at the highest Ca concentration (5,000 μg/g Ca; 51%-95% and 52 -74% lower in comparison with the control). Lead phosphate precipitates (e.g., chloropyromorphite) were observed in feces of mice fed dietary CaHPO4. In comparison, mice fed organic Ca minerals (Ca gluconate, Ca lactate, Ca aspartate, and Ca citrate) had lower duodenal mRNA expression of Ca transporters, but Pb and Cd oral bioavailability was higher than in mice fed CaHPO4. In terms of As, mice fed Ca aspartate exhibited the lowest As oral bioavailability at the highest Ca concentration (5,000 μg/g Ca; 41%-72% lower) and the lowest duodenal expression of P transporter (88% lower). The presence of aspartate was not associated with higher As solubility in the intestine. DISCUSSION: Our study used a mouse model of exposure to household dust with various concentrations and species of Ca to determine whether different Ca minerals can reduce bioavailability of Pb, Cd, and As in mice and elucidate the mechanism(s) involved. This study can contribute to the practical application of optimal Ca minerals to protect humans from Pb, Cd, and As coexposure in the environment. [ABSTRACT FROM AUTHOR]

BACKGROUND: Exposure to cadmium (Cd) is associated with cardiovascular diseases. Maternal Cd exposure is a significant risk factor for congenital heart disease. However, mechanisms of Cd on developmental cardiotoxicity are not well defined. OBJECTIVES: We evaluated the effects of Cd on the different stages (mesoderm, cardiac induction, cardiac function) of cardiac development using an early embryo development in vitro model and two- or three-dimensional (2- or 3D) cardiomyocyte and cardiac organoid formation models mimicking early cardiac development. METHODS: Embryonic stem cells (ESCs) form 3D aggregates, called embryoid bodies, that recapitulate events involved with early embryogenesis (e.g., germ layer formation). This model was used for early germ layer formation and signaling pathway identification. The 2D cardiomyocyte differentiation from the NKX2-5eGFP/w human ESCs model was used to explore the effects of Cd exposure on cardiomyocyte formation and to model mesoderm differentiation and cardiac induction, allowing us to explore different developmental windows of Cd toxicity. The 3D cardiac organoid model was used in evaluating the effects of Cd exposure on contractility and cardiac development. RESULTS: Cd (0.6 µM; 110 ppb) lowered the differentiation of embryoid bodies to mesoderm via suppression of Wnt/ß-catenin–signaling pathways. During early mesoderm induction, the mesoderm-associated transcription factors MESP1 and EOMES showed a transient up-regulation, which decreased later in the cardiac induction stage. Cd (0.15 µM) lowered mesoderm formation and cardiac induction through suppression of the transcription factors and mesoderm marker genes HAND1, SNAI2, HOPX, and the cardiac-specific genes NKX2-5, GATA4, troponin T, and alpha-actinin. In addition, Cd-induced histone modifications for both gene activation (H3K4me3) and repression (H3K27me3), which play vital roles in regulating mesoderm commitment markers. The effects of Cd inhibition on cardiomyocyte differentiation were confirmed in 3D cardiac organoids. DISCUSSION: In conclusion, using a human ESC-derived 2D/3D in vitro differentiation model system and cardiac organoids, we demonstrated that low-dose Cd suppressed mesoderm formation through mesoderm gene histone modification, thus inhibiting cardiomyocyte differentiation and cardiac induction. The studies provide valuable insights into cellular events and molecular mechanisms associated with Cd-induced congenital heart disease. [ABSTRACT FROM AUTHOR]

We have developed an innovative optical emission spectrometry imaging device integrating a diode laser for sample introduction and an atmospheric pressure plasma based on dielectric barrier discharge for atomization and excitation. By optimizing the device parameters and ensuring appropriate leaf moisture, we achieved effective imaging with a lateral resolution as low as 50 μm. This device allows for tracking the accumulation of Cd and related species such as K, Zn, and O 2 +∙, in plant leaves exposed to different Cd levels and culture times. The results obtained are comparable to established in-lab imaging and quantitative methods. With its features of compact construction, minimal sample preparation, ease of operation, and low limit of detection (0.04 μg/g for Cd), this novel methodology shows promise as an in-situ elemental imaging tool for interdisciplinary applications. [Display omitted] • Development of an OES device for in-situ elemental imaging. • Sampling with a cw laser and excitation with dielectric barrier discharge plasma. • Cost-effective and compact instrumentation. • Feasible to track Cd, K, Zn, and O 2 +∙ accumulation in plant leaves. [ABSTRACT FROM AUTHOR]

Copyright of Erzincan University Journal of Science & Technology is the property of Erzincan Binali Yildirim Universitesi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Cosmetics have been used by humans since the start of human civilization. Initially, it was typically consisting of natural products but to get prompt results, heavy metals were frequently added to cosmetics to accelerate the affects. Heavy metals such as mercury (Hg), lead (Pb), cadmium (Cd) and chromium (Cr) are detected in various cosmetic products; most frequently in color cosmetics, hair cosmetics, face-body care products and beauty cosmetic products. These metals are included in toxic metals. The application of cosmetics to different body parts leads to the absorption of metals through the stratum corneum into the blood, accumulate or replace essential elements of different biomolecules which triggers the unfavorable mechanism. Reported data shows that in some common cosmetic products toxic metals are being used greater than permissible limit. The United States Food and Drug Administration (FDA) and World Health Organization (WHO) have established the highest permissible limit of exposure for heavy metals in different cosmetic products. However, commonly in developing world no care is taken for permissible limit set by FDA and WHO and to obey cosmetic production legislations which resulted in fatal health consequences. Thus, in this review article we are focusing on the permissible limits, hazardous effects of the toxic metals and mechanisms associated with the hazardous effects related to heavy metals found in cosmetics. Owing to the growing usage of cosmetics it is necessary to explore the possible sources and routs of metals toxicity to fix the hazardous effects related to heavy metals found in cosmetics. [ABSTRACT FROM AUTHOR]

The screening and design of "green" biochar materials with high adsorption capacity play a pivotal role in promoting the sustainable treatment of Cd(II)-containing wastewater. In this study, six typical machine learning (ML) models, namely Linear Regression, Random Forest, Gradient Boosting Decision Tree, CatBoost, K-Nearest Neighbors, and Backpropagation Neural Network, were employed to accurately predict the adsorption capacity of Cd(II) onto biochars. A large dataset with 1051 data points was generated using 21 input variables obtained from batch adsorption experiments, including preparation conditions for biochar (2 features), physical properties of biochar (4 features), chemical composition of biochar (9 features), and adsorption experiment conditions (6 features). The rigorous evaluation and comparison of the ML models revealed that the CatBoost model exhibited the highest test R 2 value (0.971) and the lowest RMSE (20.54 mg/g), significantly outperforming all other models. The feature importance analysis using Shapley Additive Explanations (SHAP) indicated that biochar chemical compositions had the greatest impact on model predictions of adsorption capacity (42.2 %), followed by adsorption conditions (37.57 %), biochar physical characteristics (12.38 %), and preparation conditions (7.85 %). The optimal experimental conditions optimized by partial dependence plots (PDP) are as follows: as high Cd(II) concentration as possible, C(%) of 33 %, N(%) of 0.3 %, adsorption time of 600 min, pyrolysis time of 50 min, biochar dosage of less than 2 g/L, O(%) of 42 %, biochar pH value of 11.2, and DBE of 1.15. This study unveils novel insights into the adsorption of Cd(II) and provides a comprehensive reference for the sustainable engineering of biochars in Cd(II) wastewater treatment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Integrated health risk assessment strategies for emerging organic pollutants and heavy metals that coexist in water/soil media are lacking. Contents of perfluoroalkyl compounds and potentially toxic elements in multiple media were determined by investigating a county where a landfill and a tungsten mine coexist. The spatial characteristics and sources of contaminants were predicted by Geostatistics-based and multivariate statistical analysis, and their comprehensive health risks were assessed. The average contents of perfluorooctane acid, perfluorooctanesulfonic acid, arsenic, and cadmium in groundwater were 3.21, 0.77, 1.69, and 0.14 μg L -1 , respectively; the maximum content of cadmium in soils and rice highly reached 2.12 and 1.52 mg kg -1 , respectively. In soils, the contribution of mine lag to cadmium was 99 %, and fertilizer and pesticide to arsenic was 59.4 %. While in groundwater, arsenic, cadmium and perfluoroalkyl compounds near the landfill mainly came from leachate leakage. Significant correlations were found between arsenic in groundwater and arsenic and cadmium in soils, as well as perfluoroalkyl compounds in groundwater and pH and sulfate. Based on these correlations, the geographically optimal similarity model predicted high-level arsenic in groundwater near the tungsten mine and cadmium/perfluoroalkyl compounds around the landfill. The combination of analytic network process, entropy weighting method and game theory-based trade-off method with risk assessment model can assess the comprehensive risks of multiple pollutants. Using this approach, a high health-risk zone located around the landfill, which was mainly attributed to the presence of arsenic, cadmium and perfluorooctanesulfonic acid, was found. Overall, perfluoroalkyl compounds in groundwater altered the spatial pattern of health risks in an arsenic‑cadmium contaminated area., Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The co-contamination of soils by microplastics (MPs) and cadmium (Cd), one of the most perilous heavy metals, is emerging as a significant global concern, posing risks to plant productivity and human health. However, there remains a gap in the literature concerning comprehensive evaluations of the combined effects of MPs and Cd on soil-plant-human systems. This review examines the interactions and co-impacts of MPs and Cd in soil-plant-human systems, elucidating their mechanisms and synergistic effects on plant development and health risks. We also review the origins and contamination levels of MPs and Cd, revealing that sewage, atmospheric deposition, and biosolid applications are contributors to the contamination of soil with MPs and Cd. Our meta-analysis demonstrates that MPs significantly (p<0.05) increase the bioavailability of soil Cd and the accumulation of Cd in plant shoots by 6.9 and 9.3 %, respectively. The MPs facilitate Cd desorption from soils through direct adsorption via surface complexation and physical adsorption, as well as indirectly by modifying soil physicochemical properties, such as pH and dissolved organic carbon, and altering soil microbial diversity. These interactions augment the bioavailability of Cd, along with MPs, adversely affect plant growth and its physiological functions. Moreover, the ingestion of MPs and Cd through the food chain significantly enhances the bioaccessibility of Cd and exacerbates histopathological alterations in human tissues, thereby amplifying the associated health risks. This review provides insights into the coexistence of MPs and Cd and their synergistic effects on soil-plant-human systems, emphasizing the need for further research in this critical subject area., Competing Interests: Declaration of Competing Interest The authors declare that they have no competing interests., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Rice consumption is a major pathway for human cadmium (Cd) exposure. Understanding Cd behavior in the soil-rice system, especially under field conditions, is pivotal for controlling Cd accumulation. This study analyzed Cd concentrations and isotope compositions (δ 114/110 Cd) in rice plants and surface soil sampled at different times, along with urinary Cd of residents from typical Cd-contaminated paddy fields in Youxian, Hunan, China. Soil water-soluble Cd concentrations varied across sampling times, with δ 114/110 Cd water lighter under drained than flooded conditions, suggesting supplementation of water-soluble Cd by isotopically lighter Cd pools, increasing Cd phytoavailability. Both water-soluble Cd and atmospheric deposition contributed to rice Cd accumulation. Water-soluble Cd's contribution increased from 28-52% under flooded to 58-87% under drained conditions due to increased soil Cd phytoavailability. Atmospheric deposition's contribution (12-72%) increased with potential atmospheric deposition flux among sampling areas. The enrichment of heavy Cd isotopes occurred from root-stem-grain to prevent rice Cd accumulation. The different extent of enrichment of heavy isotopes in urine indicated different Cd exposure sources. These findings provide valuable insights into the speciation and phytoavailability changes of Cd in the soil-rice system and highlight the potential application of Cd isotopic fingerprinting in understanding the environmental fate of Cd., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The prevention of pollution requires real-time monitoring of cadmium (Cd 2+ ) concentration in the food, as it has a dramatic impact on poultry and can pose a threat to human health. Here, we fabricate a portable workstation integrating a microfluidic chip that facilitates real-time monitoring of Cd 2+ levels in real samples by utilizing the Luminol-KMnO 4 chemiluminescence (CL) system. Interestingly, Cd 2+ can significantly enhance the CL signal, resulting in sensitive detection of Cd 2+ in the range of 0-0.18 mg/L with the limit of detection (LOD) of 0.207 μg/L. Furthermore, a remote-controlled unit is integrated into the portable workstation to form a remote-controlled portable workstation (RCPW) performing automated point-of-care testing (POCT) of Cd 2+ . The as-prepared strategy allows remote control of RCPW to avoid long-distance transportation of samples to achieve real-time target monitoring. Consequently, this system furnishes RCPW for monitoring Cd 2+ levels in real samples, thereby holding potential for applications in preventing food pollution., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

The discarded longan shell-derived porous carbon material (LPC) served as a scaffold for synthesizing bismuth nanoparticle-loaded longan porous carbon nanocomposite (BiNPs@LPC) via a hydrothermal method. Then BiNPs@LPC was utilized to modify screen-printed carbon electrodes (SPCE) for simultaneous detection of Pb(II) and Cd(II) by square wave anodic stripping voltammetry (SWASV). The material was thoroughly characterized by scanning electron microscopy, X-ray diffraction, Raman spectra, Brunauer-Emmett-Teller analysis, electrochemical impedance spectroscopy and cyclic voltammetry. BiNPs@LPC exhibited abundant porous structures, high surface area, and numerous active sites, which could improve significantly response sensitivity. Under optimal conditions, the peak currents of Pb(II) and Cd(II) exhibited favorable linear relationships with the concentration within a range of 0.1-150 μg L -1 , with detection limits (S/N = 3) of 0.02 μg L -1 and 0.03 μg L -1 , respectively. BiNPs@LPC/SPCE demonstrated remarkable selectivity, stability and repeatability. The proposed method was successfully applied for the detection of Pb(II) and Cd(II) in seafoods achieving satisfying recovery of 97.8%-108.3% and 96.7%-106.4%. These excellent test properties were coupled with convenience for batch preparation of the modified electrodes, highlighting its potential for practical applications in heavy metal detection of real samples., Competing Interests: Declaration of competing interest There are no conflicts of interest to declare., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Iron (Fe) and manganese (Mn) oxides can be used to remediate Cd-polluted soils due to their excellent performance in heavy metal adsorption. However, their remediation capability is rather limited, and a higher content of available Mn and Fe in soils can reduce Cd accumulation in wheat plants due to the competitive absorption effect. In this study, goethite and cryptomelane were first respectively used to immobilize Cd in Cd-polluted weakly alkaline soils, and sodium citrate was then added to increase the content of available Mn and Fe content for further reduction of wheat Cd absorption. In the first season, the content of soil-available Cd and Cd in wheat plants significantly decreased when cryptomelane, goethite and their mixture were used as the remediation agents. Cryptomelane showed a better remediation effect, which could be attributed to its higher adsorption performance. The grain Cd content could be decreased from 0.35 mg kg -1 to 0.25 mg kg -1 when the content of cryptomelane was controlled at 0.5%. In the second season, when sodium citrate at 20 mmol kg -1 was further added to the soils with 0.5% cryptomelane treatment in the first season, the content of soil available Cd was increased by 14.8%, and the available Mn content was increased by 19.5%, leading to a lower Cd content in wheat grains (0.16 mg kg -1 ) probably due to the competitive absorption. This work provides a new strategy for the remediation of slightly Cd-polluted arable soils with safe and high-quality production of wheat., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Background: The heavy metal ion Cd 2+ is acutely toxic, and excessive concentrations can have adverse effects on human production and life, and even lead to significant public health risks and environmental impacts. There are several mature non-electrochemical methods for heavy metal detection, but these methods are characterized by high cost, which makes it difficult to be applied to the field for timely detection. Therefore, it is necessary to prepare a new electrochemical sensor that is environmentally friendly and capable of detecting Cd 2+ in the environment quickly, easily and sensitively., Results: In this study, hydrogen-bonded organic frameworks (HOFs) were synthesized by a simple hydrothermal reaction. The prepared materials consisted of only C, N and O and had a thin lamellar structure. The HOFs were integrated into a novel electrochemical sensor to achieve accurate detection of Cd 2+ ions in real aqueous environments by square wave anodic dissolution voltammetry. The sensor has a wide linear range and a detection limit as low as 0.13 μg/L. Several real water samples, such as tap water, lake water, and e-cigarette digest, were analyzed to simulate the working environment of the sensor, and the results showed that the recoveries of Cd 2+ ranged from 95.75 % to 101.2 %., Significance: We pioneered the detection of heavy metal ions Cd 2+ in e-cigarette digestate samples with the innovative use of HOFs as the sensor material, which demonstrated the potential application in electrochemical sensing with extremely low background current value and high sensitivity, providing new ideas for environmental monitoring and public health control., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The impacts of straw removal on rice Cd absorption, behaviour of Cd and microbial community in rhizosphere soil were investigated in paddy fields over two consecutive seasons. The results of the experiments in two fields revealed that straw removal promoted the transformation of soil Cd from acid-extractable and oxidisable fraction to residual fraction and reduced soil DTPA-Cd content with the reduction in DOC and Cd ions in soil porewater, thereby decreasing Cd content in rice. Specifically, the Cd content in brown rice was below 0.2 mg·kg -1 when all rice straw and roots were removed in the slightly Cd-contaminated soils. The α-diversity of soil microbial communities was less influenced by continuous straw removal, β-diversity was altered and the relative abundances of Anaeromyxobacter, Methylocystis and Mycobacterium microbes were increased. Redundancy analysis and network analysis exhibited that soil pH predominantly influenced the microbial community. Path analysis revealed that the Cd content in brown rice could be directly influenced by the soil Total-Cd and DTPA-Cd, as well as soil pH and OM. Straw removal, including roots removal, is an economical and effective technique to reduce Cd accumulation in rice plants., Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Jiao-Feng Gu reports financial support was provided by Department of Science and Technology of Hunan Province. Jiao-Feng Gu reports financial support was provided by Department of Science and Technology of Changsha City. Hang Zhou reports financial support was provided by Department of Science and Technology of Hunan Province. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Biochar is effective in mitigating heavy metal pollution, and cadmium (Cd) is the primary pollutant in agricultural fields. However, traditional trial-and-error methods for determining the optimal biochar remediation efficiency are time-consuming and inefficient because of the varied soil, biochar, and Cd pollution conditions. This study employed the machine learning method to predict the Cd immobilization efficiency of biochar in soil. The predictive accuracy of the random forest (RF) model was superior to that of the other common linear and nonlinear models. Furthermore, to improve the reliability and accuracy of the RF model, it was optimized by employing a root-mean-squared-error-based trial-and-error approach. With the aid of the optimized model, the empirical categories for soil Cd immobilization efficiency were biochar properties (60.96 %) > experimental conditions (19.6 %) ≈ soil properties (19.44 %). Finally, this study identified the optimal biochar properties for enhancing agricultural soil Cd remediation in different regions of China, which was beneficial for decision-making regarding nationwide agricultural soil remediation using biochar. The immobilization effect of alkaline biochar was pronounced in acidic soils with relatively high organic matter. This study provides insights into the immobilization mechanism and an approach for biochar selection for Cd immobilization in agricultural soil., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Cadmium(Cd) contamination can exert significantly adverse effects on soil microbiota in reclaimed areas, however, its effects on bacterial network structure are still limitedly understood. Here we collected soil samples from typical reclaimed wetlands (RW) and ditch wetlands (DW) in coastal reclamation areas and examined the effects of Cd contamination on the bacterial network complexity and stability. The results showed that the bacterial networks were destabilized by the Cd contamination, while bacteria in DW soils showed robust invulnerability characterized by higher node constancy and compositional stability compared with RW soils. Soil bacteria resisted Cd stress by forming a network with intensive connections in the module but sparser connections among the modules. Especially, network modularity was higher in DW soils than in RW soils, but made it more vulnerable to nodes removal. In addition, Cd contamination promoted bacterial positive cohesion but decreased negative cohesion in RW soils. Flavobacteriaceae, Xanthomonadaceae, and Alcaligenaceae were identified as core phylotypes, which played pivotal roles in regulating interspecies interactions due to higher contributions to cohesion and significant correlations with soil nutrients. The findings of this work indicate the changes of bacterial network structure and the indispensable role of core phylotypes in regulating interactions and maintaining network sustainability under Cd contamination., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Concentrations of toxic metals (Hg, Pb, and Cd) were determined in the muscles of Lepomis macrochirus , Barbus callensis , and Barbus nasus caught from the Moulouya River of Morocco by graphite furnace (for Pb and Cd) and cold vapour (for Hg) atomic absorption spectrometry, after acid digestion. Although the concentration of metals in some species was relatively high, no health risk has been identified in comparison to the maximum limits as set by the European Commission. The order of increasing concentrations was Cd < Hg < Pb. Calculated Target Hazard Quotients and Hazard Indices were below 1, indicating that the intake of metals via consumption of the muscles of both fish species does not represent a hazard to human health.

This research aims to assess the concentration of metals in human and canine placentas from the same geographic area and to investigate how these metal levels influence gene expression within the placenta. Placentas of 25 dogs and 60 women who had recently given birth residing in Ankara, Turkey were collected and subjected to metal analysis using ICP-OES. Placentas with detectable metal levels underwent further examination including Next Generation Sequencing, transcriptional analysis, single nucleotide polymorphism investigation, and extensive scrutiny across various groups. For women, placentas with concurrent detection of aluminum (Al), lead (Pb), and cadmium (Cd) underwent transcriptomic analysis based on metal analysis results. However, the metal load in dog placentas was insufficient for comparison. Paired-end sequencing with 100-base pair read lengths was conducted using the DNBseq platform. Sequencing quality control was evaluated using FastQC, fastq screen, and MultiQC. RNA-sequencing data is publicly available via PRJNA936158. Comparative analyses were performed between samples with detected metals and "golden" samples devoid of these metals, revealing significant gene lists and read counts. Normalization of read counts was based on estimated size factors. Principal Component Analysis (PCA) was applied to all genes using rlog-transformed count data. Results indicate that metal exposure significantly influences placental gene expression, impacting various biological processes and pathways, notably those related to protein synthesis, immune responses, and cellular structure. Upregulation of immune-related pathways and alterations in protein synthesis machinery suggest potential defense mechanisms against metal toxicity. Nonetheless, these changes may adversely affect placental function and fetal health, emphasizing the importance of monitoring and mitigating environmental exposure to metals during pregnancy., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Omnipresent in terrestrial ecosystems, microplastics (MPs) represent a hazard to soil biota and human health, while their relationship with other environmental contaminants remains poorly acknowledged. This study investigated MPs prevalence in (sub)urban soils of Serbia and its impact on Cd, As, and Pb mobility in the soil-medicinal plant Capsella bursa-pastoris (L.) Medik system. Soil physicochemical parameters (pH, Eh, SOM, and texture) were analyzed alongside the Cd, As, and Pb pseudo-total (aqua regia) and phytoavailable (EDTA) contents. Toxic elements' concentrations in soil fractions and C. bursa-pastoris roots and shoots were determined by inductively coupled plasma optical emission spectroscopy (ICP-OES). Pseudo-total Cd, As, and Pb contents in soils ranged from 0.16 to 2.23 μg g -1 , 2.00-36.92 μg g -1 , and 0.18-65.54 μg g -1 , respectively. Using an optimized density separation method with 30% H 2 O 2 and 5% NaClO, we found an average abundance of 489 MPs per kg of soil. ATR-FTIR spectroscopy confirmed the presence of seven polymer types, whereby the main contributors were polystyrene (PS) - 28.57% and cardanol prepolymer (PCP) - 23.81%. The dominant associated pollution sources were road networks and industrial activities. Spearman correlation analysis revealed the interconnection among soil MPs, physicochemical variables, and Cd, As, and Pb mobility. We identified significant positive correlations between MPs' abundance and phytoavailable concentrations of Cd, As, and Pb (ρ = 0.82, 0.95, and 0.63). Moreover, soil MPs strongly positively correlated with Cd contents in roots (ρ = 0.61) and shoots of C. bursa-pastoris (ρ = 0.65). These findings underscore the synergistic effects of MPs and toxic metals in urban environmental pollution, with possible implications for human health. Further research is required to deepen our understanding of the impact of MPs on element mobility in complex plant-soil systems and to elucidate the broader consequences of induced alterations., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Polluted environments often contain large amounts of toxic metals, such as cadmium, which pose a major threat to ecosystems and public health. Contamination by cadmium and its compounds is often observed in areas surrounding zinc mining sites and electroplating factories, and the control of cadmium pollution is essential for environmental safety and health. In this study, a highly efficient and straightforward separation strategy for K 4 Fe(CN) 6 @Fe 3 O 4 nanocomposites is successfully developed to capture the Cd ions in the water environment. Batch adsorption experiments revealed that K 4 Fe(CN) 6 @Fe 3 O 4 exhibited a high cadmium removal rate (greater than 98 %) at a pH level of 6.0 and solid-liquid ratio of 1.0 g/L at room temperature (298 K). Kinetic analysis revealed that the adsorption process followed a pseudo-second-order model and cadmium was rapidly removed in the first 10 min, with chemisorption dominating the capture of Cd 2+ by K 4 Fe(CN) 6 @Fe 3 O 4 . Adsorption isotherms revealed a heterogeneous adsorption behavior, with a maximum adsorption capacity of 40.78 mg/g. The intrinsic adsorption of Cd 2+ by K 4 Fe(CN) 6 @Fe 3 O 4 occurring primarily through electrostatic interaction and ion exchange. In addition, K 4 Fe(CN) 6 @Fe 3 O 4 exhibited an excellent regeneration capacity. Therefore, integrating Fe 3 O 4 into the metal cyanide not only provided the composite material with excellent chemical stability and selective adsorption sites for Cd 2+ , but also facilitated subsequent sorbent collection and recovery. Overall, this study presents a simple and feasible approach for integrating Fe 3 O 4 into potassium ferrocyanide frameworks for efficient cadmium removal from contaminated water., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.', (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)

Intercropping with hyperaccumulators can facilitate the safe utilization of cadmium-contaminated soil. However, the effectiveness of this approach is influenced by plant species and varieties, which necessitates research on optimal plant consortia. In this study, 8 tomato varieties (3 cherry tomatoes and 5 common large-fruit tomatoes) were intercropped with Sedum alfredii in a moderately Cd-contaminated vegetable field. The results showed that the Cd concentration in the fruits of common large-fruit tomato varieties under monoculture was 1.03-1.50 mg/kg, while that in the fruits of cherry tomato varieties was 0.67-0.71 mg/kg. After intercropping with S. alfredii, the fruit Cd concentrations of Hangza 501, Hangza 503, and Hangza 108 decreased by 16.42 %, 19.72 %, and 6.76 %, respectively, while those of the other varieties significantly increased, except for those of Hangza 8. In contrast, the shoot Cd concentration of cherry tomatoes was greater than that of large-fruit tomatoes under monoculture. Furthermore, a significant increase in the shoot Cd concentration was noted in the Hangza 501, Hangza 503 and Hangza 603 plants following intercropping. Additionally, intercropping with S. alfredii increased the concentration of soluble sugars in the fruits of Hangza 8, Hangza 501, Hangza 503 and Hangza 603 by 4.66 %, 17.91 %, 10.60 % and 17.88 %, respectively. Intercropping with tomatoes resulted in a decrease in both the biomass and Cd uptake of S. alfredii. Interestingly, the inhibitory effect on S. alfredii was less pronounced when intercropped with cherry tomatoes than when intercropped with large-fruit tomatoes. Among the intercropping treatments, S. alfredii exhibited the greatest total Cd accumulation (0.06 mg/plant) when intercropped with Hangza 503. In conclusion, the cherry tomato variety Hangza 503 was the most suitable for intercropping with S. alfredii and can be used safely for vegetable production and simultaneous phytoremediation of polluted soil. Our findings suggest that strategic selection of tomato varieties can optimize the effectiveness of "phytoextraction coupled with agro-safe production" technology for managing soil Cd concentrations., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)

The adsorption of heavy metals on iron oxides generally increases with pH and is almost complete at neutral to slightly alkaline pH. However, almost complete adsorption on a linear scale does not imply sufficient removal of the heavy metals in terms of their toxicity. Here, we elucidated the chemical reactions that determine the solid-liquid partitioning of Pb(II) and Cd(II) on goethite at high pH. While the removal of both heavy metals was almost complete on a linear scale above pH 7 for Pb(II) and pH 9 for Cd(II), the dissolved metal concentrations decreased on a logarithmic scale with pH, reaching minima at around pH 10 for Pb(II) and pH 10-11 for Cd(II), and then they increased with pH thereafter. The XAFS spectra of Pb(II)- or Cd(II)-adsorbed goethite prepared at pH > 11 were almost the same as those at neutral pH, suggesting that removal of the heavy metals from solution was achieved by a single adsorption reaction over the entire pH range. Based on the observed macroscopic and microscopic adsorption behaviors at high pH, a robust surface complexation model was developed to predict the solid-liquid partitioning of divalent heavy metals over the entire pH range., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Moso bamboo is excellent candidate for cadmium (Cd)/lead (Pb) phytoremediation, while rhizosphere microbiome has significant impact on phytoremediation efficiency of host plant. However, little is known about the rhizosphere bacterial communities of moso bamboo in Cd/Pb contaminated soils. Therefore, this study investigated the assembly patterns and key taxa of rhizosphere bacterial communities of moso bamboo in Cd/Pb polluted and unpolluted soils, by field sampling, chemical analysis, and 16S rRNA gene sequencing. The results indicated α-diversity between Cd/Pb polluted and unpolluted soils showed a similar pattern ( p  > 0.05), while β-diversity was significantly different ( p  < 0.05). The relative abundance analysis indicated α-proteobacteria (37%) and actinobacteria (31%) were dominant in Cd/Pb polluted soils, while γ-proteobacteria (40%) and α-proteobacteria (22%) were dominant in unpolluted soils. Co-occurrence network analysis indicated microbial networks were less complex and more negative in polluted soils than in unpolluted soils. Mantel analysis indicated soil available phosphorus, organic matter, and available Pb were the most important environmental factors affecting microbial community structure. Correlation analysis showed 11 bacterial genera were significantly positively related to Cd/Pb. Overall, this study identified the bacterial community composition of bamboo rhizosphere in responding to Cd/Pb contamination and provides a theoretical basis for microbe-assistant phytoremediation in the future.

Addressing cadmium (Cd) contamination in agricultural lands is crucial, given its health implications and accumulation in crops. This study used pot experiments to evaluate the impact of foliar selenium spray (Se) (0.40 mM), corn straw biochar (1%), and pig manure (1%) on the growth of rice plants, the accumulation of Cd in rice grain, and to examine their influence on health risk indices associated with Cd exposure. The treatments were designated as follows: a control group without any amendment (CK), biochar (T1), pig manure (T2), Se (T3), Se and biochar (T4), Se and pig manure (T5), and Se along with biochar and pig manure (T6). Our results indicated that the treatments affected soil pH and redox potential and improved growth and the nitrogen and phosphorus content in rice plants. The soil-plant analysis development (SPAD) meter readings of leaves during the tillering stage indicated a 5.27%-15.86% increase in treatments T2 to T6 compared to CK. The flag leaves of T2 exhibited increases of 12.06%-38.94% for electrolyte leakage and an 82.61%-91.60% decline in SOD compared to treatments T3 to T6. Treatments T1 to T6 increased protein content; however, amylose content was significantly reduced in T6. Treatment T6 recorded the lowest Cd concentration in rice grains (0.018 mg/kg), while T2 recorded the highest (0.051 mg/kg). The CK treatment group showed a grain Cd content reduction of 29.30% compared to T2. The assessment of acceptable daily intake, hazard quotient, and carcinogenic risk revealed an ascending order as follows: T6 < T3 < T5 < T4 < T1 < CK < T2. In conclusion, the application of treatment T6 demonstrates the potential to lower oxidative stress, enhance production, reduce cancer risk, and ensure the safe cultivation of rice in environments affected by Cd contamination., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier Inc.)

There is currently increasing pressure on agriculture to simultaneously remediate soil and ensure safe agricultural production. In this study, we investigate the potential of a novel combination of biochar and plant growth-promoting bacteria (PGPB) as a promising approach. Two types of biochar, corn stover and rice husk-derived, were used in combination with a PGPB strain, Bacillus sp. PGP5, to remediate Cd and Pb co-contaminated soil and enhance lettuce performance. The contaminated soil was pre-incubated with biochar prior to PGP5 inoculation. The combined application of biochar and PGPB reduced the diethylenetriaminepentaacetic acid (DTPA) -extractable Cd and Pb concentrations in the soil by 46.45%-55.96% and 42.08%-44.83%, respectively. Additionally, this combined application increased lettuce yield by 23.37%-65.39% and decreased Cd and Pb concentrations in the edible parts of the lettuce by 57.39%-68.04% and 13.57%-32.50%. The combined application showed a better promotion on lettuce growth by facilitating chlorophyll synthesis and reducing oxidative stress. These demonstrated a synergistic effect between biochar and PGPB. Furthermore, our study elucidated the specific role of the biochar-PGPB combination in soil microbial communities. Biochar application promoted the survival of PGP5 in the soil. The impact of biochar or PGPB on microbial communities was found to be most significant in the early stage, while the development of plants had a greater influence on rhizosphere microbial communities in later stage. Plants showed a tendency to recruit plant-associated microbes, such as Cyanobacteria, to facilitate growth processes. Notably, the combined application of biochar and PGPB expedited the assembly of microbial communities, enabling them more closely with the rhizosphere microbial communities in late stage of plant development and thus enhancing their effects on promoting plant growth. This study highlights the "accelerating" advantage of the biochar-PGPB combination in the assembly of rhizosphere microbiomes and offers a new strategy for simultaneous soil remediation and safe agricultural production., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Soil heavy metal contamination is often an unintended byproduct of historic land-use. This contamination can negatively impact resident plants and their interactions with other organisms. Plant fitness in contaminated landscapes depends not only on plant growth, but also on the maintenance of interactions with pollinators. Cadmium (Cd) is a heavy metal that is commonly found in agricultural, urban, and industrial ecosystems as a legacy of historic land-use. It is a prioritized pollutant in soils because of its wide distribution and strong biotoxicity. To understand how Cd influences plant growth and pollinator interactions, we grew sunflowers in media with three different Cd concentrations to represent the range of Cd contamination faced by sunflowers growing on land recovering from past land-use. We measured Cd contamination effects on sunflower morphology and pollinator foraging behavior, specifically the number of visits and visit duration. We then measured seed number and weight to determine if contamination directly or indirectly, as mediated by pollinators, altered plant fitness. Plant height was negatively correlated with Cd concentration, but contamination alone (in the absence of pollinators) did not affect sunflower reproduction. Bumble bees visited sunflowers grown in Exceeding Threshold Cd concentrations less often and for shorter time compared to visits to Below Threshold Cd sunflowers, but honey bees and sweat bees showed similar foraging behavior across Cd contamination treatment levels. Sunflower seed set was positively correlated with the total number of pollinator visits, and sunflowers grown in Exceeding Threshold Cd soil had marginally lower seed set compared to those grown in Below Threshold Cd soil. Our results suggest that at Exceeding Threshold Cd contamination levels plant-pollinator interactions are negatively affected with consequences for plant fitness., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

The effects of soil pH variations induced by submergence/drainage and biochar application on soil cadmium (Cd) availability to different rice (Oryza sativa L.) varieties are not well understood. This study aims to investigate the possible reasons for available Cd(II) reduction in paddy soil as influenced by biochar and to determine Cd(II) absorption and translocation rates in different parts of various rice varieties. A pot experiment in a greenhouse using four japonica and four indica rice varieties was conducted in Cd(II) contaminated paddy soil with peanut straw biochar. The results indicated that the submerging led to an increase in soil pH due to the consumption of protons (H + ) by the reduction reactions of iron/manganese (Fe/Mn) oxides and sulfate (SO 4 2- ) and thus the decrease in soil available Cd(II) contents. However, the drainage decreased soil pH due to the release of protons during the oxidation of Fe 2+ , Mn 2+ , and S 2- and thus the increase in soil available Cd(II) contents. Application of the biochar increased soil pH during soil submerging and inhibited the decline in soil pH during soil drainage, and thus decreased soil available Cd(II) contents under both submerging and drainage conditions. The indica rice varieties absorbed more Cd(II) in their roots and accumulated higher amounts of Cd(II) in their shoots and grains than the japonica rice varieties. The Cd(II) sensitive varieties exhibited a greater absorption and translocation rate of Cd(II) compared to the tolerant varieties of both indica and japonica rice. Biochar inhibited the absorption and accumulation of Cd(II) in the rice varieties, which ultimately lowered the Cd(II) contents in rice grains below the national food safety limit (0.2 mg kg -1 ). Overall, planting japonica rice varieties in Cd(II) polluted paddy soils combined with the use of biochar can effectively reduce Cd(II) content in rice grains which protects human health against Cd(II) toxicity., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Cadmium (Cd 2+ ) is a highly toxic heavy metal that can accumulate in the human body through contaminated food and water, posing great health risks. In this study, a label-free fluorescent aptasensor based on SYBR Green I (SGI) for the rapid and sensitive detection of Cd 2+ in food samples was designed. The aptasensor utilizes a Cd 2+ -specific aptamer (Cd-(21)) and its complementary strand (CS Cd-(21) ) to form a double-stranded DNA (dsDNA) structure in the absence of Cd 2+ . SGI intercalates into the dsDNA, resulting in a strong fluorescence signal. In the presence of Cd 2+ , the aptamer undergoes a conformational change, preventing the formation of dsDNA and leading to a decrease in fluorescence intensity. Under optimized conditions, the aptasensor exhibited a linear response to Cd 2+ concentrations ranging from 0.11 to 157.37 ng mL -1 , with a limit of detection (LOD) of 0.07 ng mL -1 . The aptasensor demonstrated high specificity and was successfully applied to detect Cd 2+ in fruits and vegetables, with satisfactory recovery rates (95-111%). The proposed aptasensor provides a promising tool for the rapid and sensitive detection of Cd 2+ in food.

Spices and herbs are susceptible to various contaminants, including heavy metals. Our study aimed to quantify the levels of lead (Pb), mercury (Hg), cadmium (As), and cadmium (Cd) in 96 composite samples of 13 herbs and spices frequently consumed in Lebanon. Twenty percent (19/96) and 4% (4/96) of the samples exceeded the permissible levels of Pb and Cd, respectively and all the samples met the permissible levels of As and Hg according to Codex Alimentarius standards. For Pb and Cd, unpackaged samples had the highest levels of unacceptable samples of 31% (8/26) and 8% (2/26), respectively. The samples locally packaged in companies with and without Food Safety Management System (FSMS) had the same levels of unacceptable samples of 12% (3/26) and 4% (1/26) for Pb and Cd, respectively. Imported packaged samples had unacceptable levels of Pb (28% (5/18)) and were acceptable for the three other heavy metals., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2024 Moussa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

In order to mitigate the risk of excessive heavy metal intake, a study was conducted to assess the levels of arsenic (As), cadmium (Cd), chromium (Cr), and lead (Pb) contamination in 23 edible seafood species obtained from markets in Haikou. The findings were analyzed to evaluate the potential health hazards posed to the local population through consumption. The metals were detected via inductively coupled plasma mass spectrometry (ICP-MS) for quantification. The non-carcinogenic and carcinogenic health risks in humans were assessed via target hazard quotient (THQ), combined target hazard quotient (CTHQ), and target cancer risk (TR). The results indicated that the rank order based on the median metal concentration was As > Cd > Cr > Pb. THQ and CTHQ showed that nine seafood species posed a non-carcinogenic risk regarding from As and Cd consumption separately, or the four targeted metals ingestion together. TR assessment indicated that the InAs in all the species presented a carcinogenic risk to coastal residents. The Cd content in bivalves, algae, and several crustacean (Mantis Shrimp, Orchid Crab, Red spot Swimming Crab) and fish species (Japanese Scad, Pacific Saury), and Cr levels in most bivalve species (Razor Clams, White Clams, Fan Shells, Oysters, Blood Clams) presented a carcinogenic risk. The As, Cd, Pb, and Cr levels of seafood in Haikou were assessed species presented a potential health risk. Necessitating stricter risk should be management and detection capability and monitoring will be improved., (© 2024. The Author(s).)

Identifying the key influencing factors in soil available cadmium (Cd) is crucial for preventing the Cd accumulation in the food chain. However, current experimental methods and traditional prediction models for assessing available Cd are time-consuming and ineffective. In this study, machine learning (ML) models were developed to investigate the intricate interactions among soil properties, climate features, and available Cd, aiming to identify the key influencing factors. The optimal model was obtained through a combination of stratified sampling, Bayesian optimization, and 10-fold cross-validation. It was further explained through the utilization of permutation feature importance, 2D partial dependence plot, and 3D interaction plot. The findings revealed that pH, surface pressure, sensible heat net flux and organic matter content significantly influenced the Cd accumulation in the soil. By utilizing historical soil surveys and climate change data from China, this study predicted the spatial distribution trend of available Cd in the Chinese region, highlighting the primary areas with heightened Cd activity. These areas were primarily located in the eastern, southern, central, and northeastern China. This study introduces a novel methodology for comprehending the process of available Cd accumulation in soil. Furthermore, it provides recommendations and directions for the remediation and control of soil Cd pollution., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Diquat (DQ) is a typical bipyridine herbicide widely used to control weeds in fields and orchards. The severe toxicity of diquat poses a serious threat to the environment and human health. Metal-organic frameworks (MOFs) have received widespread attention due to their unique physical and chemical properties and applications in the detection of toxic and harmful substances. In this work, a two-dimensional (2D) Tb(III) functionalized MOF Tb(III)@1 (1 = [Cd(HTATB)(bimb)] n ·H 2 O (Cd-MOF), H 3 TATB = 4,4',4″-triazine-2,4,6-tribenzoicacid, bimb = 1,4-bis((1H-imidazol-1-yl)methyl)benzene) has been prepared and characterized. Tb(III)@1 has excellent optical properties and high water and chemical stability. After the Tb(III) is fixed by the uncoordinated -COO- in the 1 framework, Tb(III)@1 emits the typical green fluorescence of the lanthanide ion Tb(III) through the "antenna effect". It is worth noting that Tb(III)@1 can be used as a dual emission fluorescence chemical sensor for the ratio fluorescence detection of pesticide DQ, exhibiting a relatively low detection limit of 0.06 nM and a wide detection range of 0-50 nM. After the addition of DQ, a rapid color change of Tb(III)@1 fluorescence from green to blue was observed due to the combined effects of IFE, FRET and dynamic quenching. Therefore, a simple test paper box has been designed for direct on-site determination of pesticide DQ. In addition, the developed sensor has been successfully applied to the detection of DQ in real samples (fruits a Yin-Xia Sun and Bo-Tao Ji contributed equally to this work and should be considered co-first authors.nd vegetables) with satisfactory results. The results indicate that the probe developed in this study has broad application prospects in both real sample detection and actual on-site testing., Competing Interests: Declaration of competing interest All of the authors declare that they have no conflict of interest., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Infants growing up in low- and middle-income countries are at increased risk of suffering adverse childhood experiences, including exposure to environmental pollution and lack of cognitive stimulation. In this study, we aimed to examine the levels of metals in the human milk of women living in São Paulo City, Brazil, and determine the effects on infants' neurodevelopment. For such, a total of 185 human milk samples were analyzed for arsenic (As), lead (Pb), mercury (Hg), and cadmium (Cd) using inductively coupled plasma mass spectrometry (ICP-MS). We applied the Bayley scales of infant and toddler development Third Edition (Bayley-III) to assess developmental milestones. In our analysis, we found a mean (standard deviation) concentration of As in human milk equal to 2.76 (4.09) μg L -1 , followed by Pb 2.09 (5.36) and Hg 1.96 (6.68). Cd was not detected. We observed that infants exposed to Pb presented language trajectories lower than non-exposed infants (β = -0.413; 95% CI -0.653, -0.173) after adjustment for infant age, maternal education, socioeconomic status, infant sex, and sample weights. Our results report As, Pb, and Hg contamination in human milk, and that infant exposure to Pb decreased infants' language development. These results evidence maternal-child environmental exposure and its detrimental impact on infants' health., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision., (Copyright © 2024 Naspolini, Vanzele, Tótolo, Schüroff, Fatori, Vicentini Neto, Barata-Silva, dos Santos, Fujita, Passos-Bueno, Beltrão-Braga, Campos, Carvalho, Polanczyk, Moreira and Taddei.)

Soil heavy metal pollution poses a serious threat to food security, human health, and soil ecosystems. Based on 644 soil samples collected from a typical oasis located at the eastern margin of the Tarim Basin, a series of models, namely, multiple linear regression （LR）, neural network （BP）, random forest （RF）, support vector machine （SVM）, and radial basis function （RBF）, were built to predict the soil heavy metal content. The optimal prediction result was obtained and utilized to analyze the spatial distribution features of heavy metal contamination and relevant health risks. The outcomes demonstrated that： ① The average Cd content in the study area was 0.14 mg·kg -1 , which was 1.17 times the soil background value of Xinjiang, making it the primary factor of soil heavy metal contamination in the area. Additionally, the carcinogenicity risk coefficients of Cd for both adults and children were less than 10 -4 , indicating that there were no significant long-term health risks for humans in the area. ② The estimation accuracies of the five inversion models were compared, and the validation set of the RF model had an R 2 value of 0.763 7, which was the highest among the five models. Additionally, the RMSE, MAE, and MBE of the RF model were the smallest among the five models. Therefore, the predicted values of the RF model were most consistent with the measured values of the soil Cd content. The predicted map of soil Cd distribution derived from the RF model coincided best with the interpolation map. ③ The RF model outperformed the other four models in predicting health risks associated with the soil Cd element for both adults and children, resulting in better prediction results. Comparatively, the predicted values of the LR model in the validation set varied greatly, leading to unreliable results. It was demonstrated that the RF was the best model for predicting soil Cd content and evaluating health risks in the study area, considering its superior generalization capability and anti-overfitting ability.

Electrochemical detection is favorable for the rapid and sensitive determination of heavy metal cadmium. However, the detection sensitivity needs to be further improved, and a portable, low-cost device is needed for on-site detection. Herein, an in-situ bismuth modified pre-anodized screen-printed carbon electrode (SPCE) was developed for Cd 2+ determination by square wave anodic stripping voltammetry (SWASV). The in-situ bismuth modification enhances the enrichment of Cd 2+ , and together with pre-anodization improve the electron transfer rate of electrode, thus enhancing the detection sensitivity. The electrode modification method combines pre-anodization and in-situ bismuth deposition, which is very easy and effective. Furthermore, a self-made PSoC Stat potentiostat coupled with a stirring device was fabricated for portable and low-cost electrochemical detection. After comprehensive optimization, the developed method can reach a testing time of 3 min, a detection limit of 3.55 μg/L, a linear range of 5-100 μg/L, and a recovery rate of 91.7-107.1% in water and rice samples for Cd 2+ determination. Therefore, our method holds great promise for the rapid, sensitive and on-site determination of Cd 2+ in food samples., (© 2024. The Author(s).)

The ecology of the Qinghai‒Tibet Plateau is fragile, and the ecosystems in the region are difficult to remediate once damaged. Currently, landfilling is the mainstay of domestic waste disposal in China, and numerous, widely distributed county landfills exist. trace elements (TEs) in waste are gradually released with waste degradation and cannot be degraded in nature, affecting environmental quality and human health. To reduce the chance bias that exists in studies of individual landfills, we selected 11 representative county landfills in Tibet, total of 76 soil samples were collected, eight TEs (arsenic (As), mercury (Hg), chromium (Cr), copper (Cu), lead (Pb), cadmium (Cd), nickel (Ni), and zinc (Zn)) were determined, and analysed for the current status of pollution, risk to human health, and sources of TEs to explore the impact of the landfills. The results showed that only a few landfills had individual TEs exceeding the risk screening value of the Soil Environmental Quality Risk Control Standard for Soil Contamination (GB 15618-2018) (pH > 7.5). Most of the soils around the landfills had moderate levels of pollution, but some individual landfills had higher levels, mainly due to Cd and Hg concentrations. Source analysis showed that Hg originated mainly from atmospheric transport; the other TEs came mainly from the weathering of soil parent material and bedrock. The potential risk from TEs to human health was low, and the risk to children was greater than the risk to adults. Among the three exposure routes, oral ingestion resulted in the highest carcinogenic risk and noncarcinogenic risk, with a contribution rate of more than 95%. Among the TEs, Ni had the highest carcinogenic risk, followed by Cr and As, and As had the highest noncarcinogenic risk., (© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

This study aimed to evaluate arsenic (As), cadmium (Cd), chromium (Cr), and lead (Pb) levels in dried chili peppers from nine districts in Guizhou Province. These heavy metals, widely dispersed and capable of transferring to crops, pose potential health risks to humans. The assessment included modeling daily intake (EDI), target hazard quotient (THQ), total target hazard index (TTHQ), and target carcinogenic risk (TCR) to assess health risks across different population groups. Results showed chromium (0.9540 ± 0.301 mg/kg) and lead (0.8949 ± 0.266 mg/kg) had the highest concentrations, followed by arsenic (0.3287 ± 0.093 mg/kg) and cadmium (0.0627 ± 0.017 mg/kg). Children exhibited higher EDI values than adults, indicating greater health risks from dried chili pepper consumption at equivalent levels. THQ and TTHQ values were below 1 across all regions, indicating no significant health risks associated with dried chili pepper consumption. Similarly, TCR values were below 10-4 for all nine regions, indicating an acceptable level of carcinogenic risk. Overall, consuming dried chili peppers in Guizhou Province poses an acceptable health risk, but caution is advised, especially for children, to limit heavy metal exposure., (© 2024. The Author(s).)

To enhance the remediation effect of heavy metal pollution, organic fertilizers with different maturity levels were added to cadmium-contaminated soil. The remediation effect was determined by evaluating the form transformation and bioavailability of cadmium in heavy metal-contaminated soil. -Results showed that when the maturity was 50%, although the soil humus (HS) content increased, it didn't contribute to reducing the bioavailability of soil Cd. Appropriately increasing the maturity (GI ≥ 80%), the HS increased by 113.95%∼157.96%, and reduced significantly the bioavailability of soil Cd, among the exchangeable Cd decreased by 16.04%∼33.51% (P < 0.01). The structural equation modeling (SEM) revealed that HS content is a critical factor influencing the transformation of Cd forms and the reduction of exchangeable Cd accumulation; the HS and residual Cd content were positively correlated with the maturity (P < 0.01), while exchangeable Cd content was negatively correlated with maturity (P < 0.01), and the correlation increased with increasing maturity. In summary, appropriately increasing the maturity (GI ≥ 80%) can increase significantly HS, promote the transformation of exchangeable Cd into residual Cd, and ultimately enhance the effectiveness of organic fertilizers in the remediation of soil Cd pollution. These results provide a new insight into the remediation of Cd-contaminated soil through organic fertilizer as soil amendment in Cd-contaminated soil., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier Ltd.)

Cadmium is a toxic heavy metal, which is both an environmental pollutant, and a threat to human health. A fluorescent probe was developed to detect Cd 2+ selectively, sensitively, and quickly. This study reports the successful development of a polypeptide fluorescent probe TPE-HC (TPE-His-Pro-Gly-Cys) which selectively detects Cd 2+ by Aggregation-Induced Emission effect. After fluorescence excitation, Cd 2+ can be effectively detected based on the change of fluorescence intensity. The detection limit of Cd 2+ in buffer solution was determined to be 151 nM (R 2  = 0.9933). This probe exhibits high sensitivity, high cell permeabilit y, and low biological toxicity, and can perform live cell imaging under biological conditions. This study indicates that TPE-HC can detect Cd 2+ in biological environments., (© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)

The majority of the so-called heavy metals are suspected to be involved in a number of pathologies and play a role in human carcinogenesis. Some of them (i.e. arsenic (As), cadmium (Cd), chromium (Cr), lead (Pb), mercury (Hg) and nickel (Ni)) have been defined as carcinogens, increasing the susceptibility of tumor development and progression in humans. Moreover, Ni, Cr, Cd, Hg, and Pb together with zinc (Zn) and iron (Fe), may be capable of stimulating the progression of breast cancer and reducing a patient's sensitivity to treatment through alterations to DNA methylation. In patients with gastric cancers, levels of various heavy metals are augmented and hypothesized to amplify the expression of the human epidermal growth factor receptor type 2 gene. Cd may increase the risk of lung cancer development and have a negative impact on the overall survival of lung cancer patients. To investigate the relation between heavy metals in biological samples and risk, occurrence and survival cancer individuals, a comprehensive review work was performed, with a focus on breast, lung, prostate and gastric cancers. An extensive search strategy was devised to ensure relevant literature could be identified, with the PECO framework being adopted to facilitate this and identify key search terms. As evidenced in this review, there is substantial data to support the hypothesis that heavy metals influence tumor development and progression. Unluckily the number of papers dealing with the determination of metals directly in samples from cancer tissues is still rather limited, so we decided to expand the scope of this review also to analyses carried out on other biological samples, as urine, plasma, hair, nail, etc. The studies reviewed showed that several limitations and current knowledge gaps are present in the literature that require further investigation to improve our comprehension of the impact of different heavy metals on tumorigenesis., (© 2024. The Author(s).)

As many contaminated agricultural soils can no longer be used for food crops, lignocellulosic energy crops matter due to their ability to grow on such soils and to produce biomass for biosourced materials and biofuels, thereby reducing the pressure on the limited arable lands. Sorghum bicolor (L.) Moench, can potentially produce a high biomass suitable for producing bioethanol, renewable gasoline, diesel, and sustainable aircraft fuel, despite adverse environmental conditions (e.g. drought, contaminated soils). A 2-year field trial was carried out for the first time in the northern France for assessing sorghum growth on a Cd, Pb and Zn-contaminated agricultural soil amended with humic/fulvic acid, alone and paired with arbuscular mycorrhizal fungi. Sorghum produced on average (in t DW ha -1 ): 12.4 in year 1 despite experiencing a severe drought season and 15.3 in year 2. Humic/fulvic acids (Lonite 80SP®) and arbuscular mycorrhizal fungi did not significantly act as biostimulants regarding the shoot DW yield and metal uptake of sorghum. The annual shoot Cd, Pb and Zn removals averaged 0.14, 0.20 and 1.97 kg ha -1 , respectively. Sorghum cultivation and its metal uptake induced a significant decrease in 0.01 M Ca(NO 3 ) 2 -extractable soil Cd, Pb and Zn concentrations by 95%, 73% and 95%, respectively, in year 2. Soluble and exchangeable soil Cd, Pb and Zn would be progressively depleted in subsequent crops, which should result in lower pollutant linkages and enhanced ecosystem services. This evidenced sorghum as a relevant plant species for phytomanaging the large area (750 ha) with metal-contaminated soil near the former Pb/Zn Metaleurop Nord smelter, amidst ongoing climate change. The potential bioethanol yield of the harvested sorghum biomass was 5589 L ha -1 . Thus sorghum would be a promising candidate for bioethanol production, even in this northern French region., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

The assessment of human health risk due to the presence of hazardous elements in the environment is now necessary for environmental management and legislative initiatives. This study aims to determine the contamination by As, Cd, Pb, and Cr in soils near gold mines in three municipalities located in the Andean region of Colombia. One of the main objectives of the study is to explore possible correlations between the Lifetime Cancer Risk (LCR) and phytotoxicity biomarkers using a simple and rapid-response plant model, radish (Raphanus sativus L.). In the municipality of Yalí, Puerto Berrío, and Buriticá, the hazardous elements concentrations ranged from 8.1 to 35.5, 1.7 to 892, and 5.8 to 49.8 for As, 0.1 to 4.6, 0.1 to 65.2, and 0.5 to 18.2 for Cd, 18.5 to 201.3, 13.0 to 1908, and 189 to 2345 for Pb, and 5.4 to 118.4, 65.4 to 301, and 5.4 to 102.3 for Cr, respectively. The results showed that the biomarkers intracellular H 2 O 2 concentration, antioxidant activity, and radicle elongation exhibited significant (P < 0.05) variations associated with the concentration of hazardous elements in the soils. Significant correlations (P < 0.05, r > 0.58) were found between the biomarkers and the LCR for Cd, Pb, and Cr, but not for As. The results using biomarkers reveal that soil pH and organic matter content are important variables that control the bioavailability of these elements in the soil. The use of indicators like LCR alone has limitations and should be accompanied by the use of biomarkers that allow for a better understanding of the biological system's response to exposure to potentially toxic elements. The results obtained show the urgent need to implement public policies to minimize exposure to hazardous substances in areas near gold mining projects., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Julián E. López reports financial support was provided by Institución Universitaria Colegio Mayor de Antioquia. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier Ltd.)

Excessive cadmium (Cd) concentration in wheat grain is becoming a widespread concern in China. Considering the complexity of Cd transfer in the soil-wheat system, how the Cd risk in wheat grain be accurately predicted from the limited details available is of great significance for the risk management of Cd. Bayes' theory could leverage existing data by combining prior information and observational data, providing a promising strategy with which to calculate a more robust posterior probability of a grain sample exceeding the food safety standard (FSS) for Cd (0.1 mg kg -1 ). In the current study, a risk prediction model, based on Bayes' theory, was established to achieve a more accurate prediction of the wheat grain Cd risk from a limited number of soil parameters. The risk prediction model could predict the risk probability of wheat grain with a Cd concentration exceeding the FSS under a given soil concentration of either total Cd or diethylenetriaminepentaacetic acid (DTPA)-extractable Cd. Soil total Cd concentration proved to be a better variable for the model with greater predictive accuracy. The model predicted that fewer than 5% of the wheat grain would have a Cd concentration exceeding the FSS when grown in soil with a total Cd concentration of less than 0.299 mg kg -1 . The risk probability rose significantly to 50% when the soil total Cd reached 0.778 mg kg -1 . The accuracy of the model was greater than the widely applied multiple linear regression model, whereas previously published data from similar soil conditions also confirmed that the Bayesian model could predict wheat Cd risk with minimal error. The proposed model provides an accurate, accessible and cost-effective methodology for predicting Cd risk in wheat grown in alkaline soils before harvest. The wider application to other soil conditions, crops or contaminants using the Bayesian model is also promising for risk management authorities., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Hair is good bioindicator of exposure, due to its ability to store and retain trace elements for long periods of time. But it can be especially useful when hair dyes are used since they may contain potentially toxic salts in their composition. In this context, analytical methods for the determination of bismuth, cadmium, lead, and silver in scalp human hair by electrothermal atomic absorption spectrometry were successfully validated. A total of 60 samples obtained from women between 18 and 60 years were analyzed: 34 dyed hairs and 26 untreated hairs (control). Average results expressed in dry weight (dyed/control) for each element were 2.34/0.49 μg g -1 (silver), 0.142/0.139 μg g -1 (bismuth), 0.055/0.054 μg g -1 (cadmium), and 2.09/0.99 μg g -1 (lead), respectively. These results agreed with those previously reported for non-exposed populations. A statistically significant higher Ag concentration in dyed hairs was observed, suggesting the bioaccumulation of this element. The associations between metal concentration and variables of interest (age, education, smoking habit, dye brand, use of dietary supplements) were investigated. A strong Pearson correlation was found for the pair Ag/Pb (r = 0.494, p < 0.05). Also, strong associations between lead levels and all the selected variables were observed (p < 0.05), while strong associations between silver levels with age and dye brand and association between cadmium levels and smoking habit were found. Furthermore, several commercial hair dye brands were analyzed to verify compliance with cosmetic regulations. This constitutes the first study of such characteristics performed in Uruguay, with worldwide relevance., (© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

Biochar has shown promising potential for soil remediation, yet its impact on heavy metals (HMs) immobilization often overlooks soil structure, which could influence soil cracking behavior and HMs transport. To address this gap, this study investigates the role of soil structure (dry density and aggregate size) on the cracking and cadmium (Cd) leaching behavior of biochar-amended fine-grained soils. A series of semi-dynamic leaching tests were conducted on samples with and without wetting-drying (W-D) cycles. Based on the proposed improved method for quantifying the effective diffusion coefficient (D e ) of Cd in unsaturated soils and microstructural analyses, we found that: (1) Higher dry density and larger aggregate generally resulted in smaller D e by decreasing soil pore volume. (2) Biochar could connect isolated pores within large aggregates through its internal pores, yielding greater increases in D e (294.8%-469.0%) compared to small aggregates (29.1%-77.4%) with 3% biochar. However, further increases in biochar dosage led to decreased D e , primarily due to the dense pore structure. (3) Biochar effectively inhibited soil cracking, achieving the highest reduction of 36.8% in surface crack ratio. (4) After W-D cycles, samples exhibited higher D e with increasing dry density, with aggravated cracking being the primary cause, suggesting preferential flow within the cracks, particularly those penetrating the soil. This study highlights the importance of careful consideration of soil structure and cracking potential before in situ field application of biochar as a remediation agent for HMs-contaminated fine-grained soils., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

The significant increase in cadmium (Cd) and lead (Pb) pollution in agricultural soil has greatly heightened environmental contamination issues and the risk of human diseases. However, the mechanisms underlying the transformation of Cd and Pb in soil as well as the influencing factors during their accumulation in crop grains remain unclear. Based on the analysis of the distribution trend of Cd and Pb in soil during the growth and development stages of wheat (tillering, filling, and maturity) in alkaline heavy metal-polluted farmland in northern China, this study investigated the response mechanism of soil heavy metal form transformation to soil physicochemical properties, and elucidated the main determining periods and influencing factors for Cd and Pb enrichment in wheat grains. The results showed that an increase in CEC and SOM levels, along with a decrease in pH level, contributed to enhancing the bioavailability of Cd in the soil. This effect was particularly evident during the tillering stage and grain filling stage of wheat. Nevertheless, the effects of soil physicochemical properties on bioavailable Pb was opposite to that on bioavailable Cd. The enrichment of Cd and Pb in grain was significantly influenced by soil pH (r = -0.786, p < 0.01), SOM (r = 0.807, p < 0.01), K (r = -0.730, p < 0.01), AK (r = 0.474, p = 0.019), and AP (r = -0.487, p = 0.016). The reducible form of Cd in soil during the wheat tillering stage was identified as the primary factor contributing to the accumulation of Cd and Pb in wheat grains, with a significant contribution rate of 84.5%. This study provides a greater scientific evidence for the management and risk control of heavy metal pollution in alkaline farmland., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)