314 results on '"Caño-Delgado A"'
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2. Analysis of expressed sequence tags generated from full-length enriched cDNA libraries of melon
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Bendahmane Abdelhafid, Katzir Nurit, Caño-Delgado Ana I, Mascarell-Creus Albert, Portnoy Vitaly, Dolcet-Sanjuan Ramon, Hernandez-Gonzalez Maria, Boualem Adnane, Huang Mingyun, Truniger Veronica, Jublot Delphine, Zheng Yi, Joobeur Tarek, Clepet Christian, Giovannoni James J, Aranda Miguel A, Garcia-Mas Jordi, and Fei Zhangjun
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Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Melon (Cucumis melo), an economically important vegetable crop, belongs to the Cucurbitaceae family which includes several other important crops such as watermelon, cucumber, and pumpkin. It has served as a model system for sex determination and vascular biology studies. However, genomic resources currently available for melon are limited. Result We constructed eleven full-length enriched and four standard cDNA libraries from fruits, flowers, leaves, roots, cotyledons, and calluses of four different melon genotypes, and generated 71,577 and 22,179 ESTs from full-length enriched and standard cDNA libraries, respectively. These ESTs, together with ~35,000 ESTs available in public domains, were assembled into 24,444 unigenes, which were extensively annotated by comparing their sequences to different protein and functional domain databases, assigning them Gene Ontology (GO) terms, and mapping them onto metabolic pathways. Comparative analysis of melon unigenes and other plant genomes revealed that 75% to 85% of melon unigenes had homologs in other dicot plants, while approximately 70% had homologs in monocot plants. The analysis also identified 6,972 gene families that were conserved across dicot and monocot plants, and 181, 1,192, and 220 gene families specific to fleshy fruit-bearing plants, the Cucurbitaceae family, and melon, respectively. Digital expression analysis identified a total of 175 tissue-specific genes, which provides a valuable gene sequence resource for future genomics and functional studies. Furthermore, we identified 4,068 simple sequence repeats (SSRs) and 3,073 single nucleotide polymorphisms (SNPs) in the melon EST collection. Finally, we obtained a total of 1,382 melon full-length transcripts through the analysis of full-length enriched cDNA clones that were sequenced from both ends. Analysis of these full-length transcripts indicated that sizes of melon 5' and 3' UTRs were similar to those of tomato, but longer than many other dicot plants. Codon usages of melon full-length transcripts were largely similar to those of Arabidopsis coding sequences. Conclusion The collection of melon ESTs generated from full-length enriched and standard cDNA libraries is expected to play significant roles in annotating the melon genome. The ESTs and associated analysis results will be useful resources for gene discovery, functional analysis, marker-assisted breeding of melon and closely related species, comparative genomic studies and for gaining insights into gene expression patterns.
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- 2011
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3. An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (Cucumis melo L.)
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Garcia-Mas Jordi, Aranda Miguel A, Picó-Silvent Belén, López-Bigas Nuria, Deleu Wim, Roig Cristina, Saladié Montserrat, Gonzalez-Ibeas Daniel, Blanca José, Mora-García Santiago, Vilarrasa-Blasi Josep, Cañizares Joaquin, Mascarell-Creus Albert, Nuez Fernando, Puigdomènech Pere, and Caño-Delgado Ana I
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Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Melon (Cucumis melo) is a horticultural specie of significant nutritional value, which belongs to the Cucurbitaceae family, whose economic importance is second only to the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic diversity has prompted the development of genetic tools in the last decade. However, the unprecedented existence of a transcriptomic approaches in melon, highlight the importance of designing new tools for high-throughput analysis of gene expression. Results We report the construction of an oligo-based microarray using a total of 17,510 unigenes derived from 33,418 high-quality melon ESTs. This chip is particularly enriched with genes that are expressed in fruit and during interaction with pathogens. Hybridizations for three independent experiments allowed the characterization of global gene expression profiles during fruit ripening, as well as in response to viral and fungal infections in plant cotyledons and roots, respectively. Microarray construction, statistical analyses and validation together with functional-enrichment analysis are presented in this study. Conclusion The platform validation and enrichment analyses shown in our study indicate that this oligo-based microarray is amenable for future genetic and functional genomic studies of a wide range of experimental conditions in melon.
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- 2009
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4. MELOGEN: an EST database for melon functional genomics
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Puigdomènech Pere, Garcia-Mas Jordi, Nuez Fernando, Arús Pere, Caño-Delgado Ana, Deleu Wim, Gómez Pedro, Truniger Verónica, González-To Mireia, Picó Belén, Roig Cristina, Blanca José, Gonzalez-Ibeas Daniel, and Aranda Miguel A
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Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Melon (Cucumis melo L.) is one of the most important fleshy fruits for fresh consumption. Despite this, few genomic resources exist for this species. To facilitate the discovery of genes involved in essential traits, such as fruit development, fruit maturation and disease resistance, and to speed up the process of breeding new and better adapted melon varieties, we have produced a large collection of expressed sequence tags (ESTs) from eight normalized cDNA libraries from different tissues in different physiological conditions. Results We determined over 30,000 ESTs that were clustered into 16,637 non-redundant sequences or unigenes, comprising 6,023 tentative consensus sequences (contigs) and 10,614 unclustered sequences (singletons). Many potential molecular markers were identified in the melon dataset: 1,052 potential simple sequence repeats (SSRs) and 356 single nucleotide polymorphisms (SNPs) were found. Sixty-nine percent of the melon unigenes showed a significant similarity with proteins in databases. Functional classification of the unigenes was carried out following the Gene Ontology scheme. In total, 9,402 unigenes were mapped to one or more ontology. Remarkably, the distributions of melon and Arabidopsis unigenes followed similar tendencies, suggesting that the melon dataset is representative of the whole melon transcriptome. Bioinformatic analyses primarily focused on potential precursors of melon micro RNAs (miRNAs) in the melon dataset, but many other genes potentially controlling disease resistance and fruit quality traits were also identified. Patterns of transcript accumulation were characterised by Real-Time-qPCR for 20 of these genes. Conclusion The collection of ESTs characterised here represents a substantial increase on the genetic information available for melon. A database (MELOGEN) which contains all EST sequences, contig images and several tools for analysis and data mining has been created. This set of sequences constitutes also the basis for an oligo-based microarray for melon that is being used in experiments to further analyse the melon transcriptome.
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- 2007
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5. The IFMIF-DONES remote handling control system: Experimental setup for OPC UA integration
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Valenzuela, E., Cano-Delgado, A., Cruz-Miranda, J., Rouret, M., Miccichè, G., Ros, E., Arranz, F., and Diaz, J.
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- 2023
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6. Analysis of metabolic dynamics during drought stress in Arabidopsis plants
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Lozano-Elena, Fidel, Fàbregas, Norma, Coleto-Alcudia, Veredas, and Caño-Delgado, Ana I.
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- 2022
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7. Molecular and physiological characterization of brassinosteroid receptor BRI1 mutants in Sorghum bicolor
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Rico-Medina, Andrés, primary, Blasco-Escámez, David, additional, Fontanet-Manzaneque, Juan B, additional, Laibach, Natalie, additional, Lozano-Elena, Fidel, additional, Martignago, Damiano, additional, and Caño-Delgado, Ana, additional
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- 2024
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8. A Method for Rapid and Reliable Molecular Detection of Drought-Response Genes in Sorghum bicolor (L.) Moench Roots
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Fontanet-Manzaneque, Juan B., primary, Blasco-Escámez, David, additional, Martignago, Damiano, additional, Rico-Medina, Andrés, additional, and Caño-Delgado, Ana I., additional
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- 2022
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9. MyROOT 2.0: An automatic tool for high throughput and accurate primary root length measurement
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González, Alejandro, Sevillano, Xavier, Betegón-Putze, Isabel, Blasco-Escámez, David, Ferrer, Marc, and Caño-Delgado, Ana I.
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- 2020
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10. Precise transcriptional control of cellular quiescence by BRAVO/WOX5 complex in Arabidopsis roots
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Isabel Betegón‐Putze, Josep Mercadal, Nadja Bosch, Ainoa Planas‐Riverola, Mar Marquès‐Bueno, Josep Vilarrasa‐Blasi, David Frigola, Rebecca C Burkart, Cristina Martínez, Ana Conesa, Rosangela Sozzani, Yvonne Stahl, Salomé Prat, Marta Ibañes, and Ana I Caño‐Delgado
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BRAVO ,mathematical modelling ,quiescent centre ,root growth ,WOX5 ,Biology (General) ,QH301-705.5 ,Medicine (General) ,R5-920 - Abstract
Abstract Understanding stem cell regulatory circuits is the next challenge in plant biology, as these cells are essential for tissue growth and organ regeneration in response to stress. In the Arabidopsis primary root apex, stem cell‐specific transcription factors BRAVO and WOX5 co‐localize in the quiescent centre (QC) cells, where they commonly repress cell division so that these cells can act as a reservoir to replenish surrounding stem cells, yet their molecular connection remains unknown. Genetic and biochemical analysis indicates that BRAVO and WOX5 form a transcription factor complex that modulates gene expression in the QC cells to preserve overall root growth and architecture. Furthermore, by using mathematical modelling we establish that BRAVO uses the WOX5/BRAVO complex to promote WOX5 activity in the stem cells. Our results unveil the importance of transcriptional regulatory circuits in plant stem cell development.
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- 2021
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11. MyROOT 2.0: An automatic tool for high throughput and accurate primary root length measurement.
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Alejandro González, Xavier Sevillano, Isabel Betegón-Putze, David Blasco-Escámez, Marc Ferrer, and Ana I. Caño-Delgado
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- 2020
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12. Heterodimerization and Endocytosis of Arabidopsis Brassinosteroid Receptors BRI1 and AtSERK3 (BAK1)
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Russinova, Eugenia, Borst, Jan-Willem, Kwaaitaal, Mark, Caño-Delgado, Ana, Yin, Yanhai, Chory, Joanne, and de Vries, Sacco c.
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- 2004
13. New methods for sorghum transformation in temperate climates
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Miller, Sara, primary, Rønager, Asta, additional, Holm, Rose, additional, Fontanet-Manzaneque, Juan B, additional, Caño-Delgado, Ana I, additional, and Bjarnholt, Nanna, additional
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- 2023
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14. Extragenic Suppressors of the Arabidopsis gai Mutation Alter the Dose-Response Relationship of Diverse Gibberellin Responses
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Peng, Jinrong, Richards, Donald E., Moritz, Thomas, Caño-Delgado, Ana, and Harberd, Nicholas P.
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- 1999
15. PloidyQuantX: A Quantitative Microscopy Imaging Tool for Ploidy Quantification at Cell and Organ Level in Arabidopsis Root.
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Xavier Sevillano, Marc Ferrer, Mary-Paz González-García, Irina Pavelescu, and Ana I. Caño-Delgado
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- 2015
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16. PloidyQuantX: A Quantitative Microscopy Imaging Tool for Ploidy Quantification at Cell and Organ Level in Arabidopsis Root
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Sevillano, Xavier, Ferrer, Marc, González-García, Mary-Paz, Pavelescu, Irina, Caño-Delgado, Ana I., Hutchison, David, Series editor, Kanade, Takeo, Series editor, Kittler, Josef, Series editor, Kleinberg, Jon M., Series editor, Kobsa, Alfred, Series editor, Mattern, Friedemann, Series editor, Mitchell, John C., Series editor, Naor, Moni, Series editor, Nierstrasz, Oscar, Series editor, Pandu Rangan, C., Series editor, Steffen, Bernhard, Series editor, Terzopoulos, Demetri, Series editor, Tygar, Doug, Series editor, Weikum, Gerhard, Series editor, Istrail, Sorin, Series editor, Pevzner, Pavel, Series editor, Waterman, Michael S., Series editor, Ortuño, Francisco, editor, and Rojas, Ignacio, editor
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- 2015
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17. A diffusion-driven switch specifies rhizoid precursor cells inMarchantia polymorpha
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Mercadal, Josep, primary, Ferreira-Guerra, Mar, additional, Caño-Delgado, Ana, additional, and Ibañes, Marta, additional
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- 2023
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18. Brassinosteroid receptor BRL3 triggers systemic plant adaptation to elevated temperature from the phloem cells
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Gupta, Aditi, primary, Rico-Medina, Andrés, additional, Lozano-Elena, Fidel, additional, Marqués-Bueno, Mar, additional, Fontanet, Juan B., additional, Fàbregas, Norma, additional, Alseekh, Saleh, additional, Fernie, Alisdair R., additional, and Caño-Delgado, Ana I., additional
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- 2023
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19. Overexpression of the vascular brassinosteroid receptor BRL3 confers drought resistance without penalizing plant growth
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Fàbregas, Norma, Lozano-Elena, Fidel, Blasco-Escámez, David, Tohge, Takayuki, Martínez-Andújar, Cristina, Albacete, Alfonso, Osorio, Sonia, Bustamante, Mariana, Riechmann, José Luis, Nomura, Takahito, Yokota, Takao, Conesa, Ana, Alfocea, Francisco Pérez, Fernie, Alisdair R., Caño-Delgado, Ana I., Fàbregas, Norma, Lozano-Elena, Fidel, Blasco-Escámez, David, Tohge, Takayuki, Martínez-Andújar, Cristina, Albacete, Alfonso, Osorio, Sonia, Bustamante, Mariana, Riechmann, José Luis, Nomura, Takahito, Yokota, Takao, Conesa, Ana, Alfocea, Francisco Pérez, Fernie, Alisdair R., and Caño-Delgado, Ana I.
- Abstract
Drought represents a major threat to food security. Mechanistic data describing plantresponses to drought have been studied extensively and genes conferring drought resistancehave been introduced into crop plants. However, plants with enhanced drought resistanceusually display lower growth, highlighting the need for strategies to uncouple droughtresistance from growth. Here, we show that overexpression of BRL3, a vascular-enrichedmember of the brassinosteroid receptor family, can confer drought stress tolerance inArabidopsis. Whereas loss-of-function mutations in the ubiquitously expressed BRI1 receptorleads to drought resistance at the expense of growth, overexpression of BRL3 receptorconfers drought tolerance without penalizing overall growth. Systematic analyses reveal thatupon drought stress, increased BRL3 triggers the accumulation of osmoprotectant metabo-lites including proline and sugars. Transcriptomic analysis suggests that this results fromdifferential expression of genes in the vascular tissues. Altogether, this data suggests thatmanipulating BRL3 expression could be used to engineer drought tolerant crops.
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- 2023
20. New methods for sorghum transformation in temperate climates
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Villum Fonden, European Commission, Miller, Sara, Rønager, Asta, Holm, Rose, Fontanet-Manzaneque, Juan B., Caño-Delgado, Ana I., Bjarnholt, Nanna, Villum Fonden, European Commission, Miller, Sara, Rønager, Asta, Holm, Rose, Fontanet-Manzaneque, Juan B., Caño-Delgado, Ana I., and Bjarnholt, Nanna
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Sorghum (Sorghum bicolor) is an emerging cereal crop in temperate climates due to its high drought tolerance and other valuable traits. Genetic transformation is an important tool for the improvement of cereals. However, sorghum is recalcitrant to genetic transformation which is almost only successful in warmer climates. Here, we test the application of two new techniques for sorghum transformation in temperate climates, namely transient transformation by Agrobacterium tumefaciens-mediated agroinfiltration and stable transformation using gold particle bombardment and leaf whorls as explants. We optimized the transient transformation method, including post-infiltration incubation of plants in the dark and using Agrobacterium grown on plates with a high cell density (OD600 = 2.0). Expression of the green fluorescence protein (GFP)-tagged endogenous sorghum gene SbDHR2 was achieved with low transformation efficiency, and our results point out a potential weakness in using this approach for localization studies. Furthermore, we succeeded in the production of callus and somatic embryos from leaf whorls, although no genetic transformation was accomplished with this method. Both methods show potential, even if they seem to be influenced by climatic conditions and therefore need further optimization to be applied routinely in temperate climates.
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- 2023
21. New methods for sorghum transformation in temperate climates
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Miller, Sara, Rønager, Asta, Holm, Rose, Fontanet-Manzaneque, Juan B., Caño-Delgado, Ana I., Bjarnholt, Nanna, Miller, Sara, Rønager, Asta, Holm, Rose, Fontanet-Manzaneque, Juan B., Caño-Delgado, Ana I., and Bjarnholt, Nanna
- Abstract
Sorghum (Sorghum bicolor) is an emerging cereal crop in temperate climates due to its high drought tolerance and other valuable traits. Genetic transformation is an important tool for the improvement of cereals. However, sorghum is recalcitrant to genetic transformation which is almost only successful in warmer climates. Here, we test the application of two new techniques for sorghum transformation in temperate climates, namely transient transformation by Agrobacterium tumefaciens–mediated agroinfiltration and stable transformation using gold particle bombardment and leaf whorls as explants. We optimized the transient transformation method, including post-infiltration incubation of plants in the dark and using Agrobacterium grown on plates with a high cell density (OD600 = 2.0). Expression of the green fluorescence protein (GFP)-tagged endogenous sorghum gene SbDHR2 was achieved with low transformation efficiency, and our results point out a potential weakness in using this approach for localization studies. Furthermore, we succeeded in the production of callus and somatic embryos from leaf whorls, although no genetic transformation was accomplished with this method. Both methods show potential, even if they seem to be influenced by climatic conditions and therefore need further optimization to be applied routinely in temperate climates.
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- 2023
22. A diffusion-driven switch specifies rhizoid precursor cells inMarchantia polymorpha
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Josep Mercadal, Mar Ferreira-Guerra, Ana I. Caño-Delgado, and Marta Ibañes
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The specification of rhizoid precursor cells in the epidermis ofMarchantia polymorphagemmae has been shown to involve lateral inhibition mediated by the microRNA FRH1, which represses its activator RSL1, a rhizoidspecific transcription factor. However, how inhibition is conferred to adjacent cells and which is the mechanism underlying the emergence of rhizoid precursors remain unknown. In this paper, we use mathematical and computational modeling to show that the previously reported rhizoid patterns in WT, gain-of-function and lossof-function mutants ofFRH1andRSL1are consistent with lateral inhibition mediated by a mobile FRH1. Our modeling results suggest that cells inMarchantiawildtype gemmae reside close to a critical state, where diffusion of FRH1 drives a switch of RSL1 expression that specifies rhizoid precursors. This process involves an initially random trigger and subsequent lateral inhibition, leading to cellular patterns consisting of small and filamentous clusters of rhizoid precursors. We confirm these predictions with new data on WT rhizoid distributions. Our findings highlight a novel mechanism of cellular pattern formation, opening new research directions for understanding cellular differentiation and tissue morphogenesis, with potential implications for a broad range of biological systems.
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- 2023
23. Brassinosteroid receptor BRL3 triggers systemic plant adaptation to elevated temperature from the phloem cells
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Aditi Gupta, Andrés Rico-Medina, Fidel Lozano-Elena, Mar Marqués-Bueno, Juan B. Fontanet, Norma Fàbregas, Saleh Alseekh, Alisdair R. Fernie, and Ana I. Caño-Delgado
- Abstract
Understanding plant receptor signaling is crucial to mitigate climate change impact on agriculture. BRs bind to membrane receptor-kinase BR-INSENSITIVE 1 (BRI1) in most plant cells that is essential to promote growth and stress responses, while the roles of vascular BRI1-LIKE1 and 3 (BRL3) receptors were considered redundant. While going unnoticed for twenty years, our study unveils thatbrl3mutants show conditional phenotypes to climate stress factors such as elevated temperatures, water deprivation and rising CO2levels. In response to adverse climate conditions such as elevated temperature, BRL3 signaling at the phloem-companion cells can promote growth by activating BRI1-EMS-SUPPRESSOR1 (BES1) effector, hormonal homeostasis, and central carbon metabolism. This study shifts the paradigm for our present understanding of BR signaling and opens innovative strategies to develop climate-smart crops.One sentence summaryPhloem-specific BRL3 receptor pathway controls plant adaption to elevated temperature.
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- 2023
24. BES1 regulates the localization of the brassinosteroid receptor BRL3 within the provascular tissue of the Arabidopsis primary root
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Salazar-Henao, Jorge E., Lehner, Reinhard, Betegón-Putze, Isabel, Vilarrasa-Blasi, Josep, and Caño-Delgado, Ana I.
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- 2016
25. Overexpression of the vascular brassinosteroid receptor BRL3 confers drought resistance without penalizing plant growth
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Fàbregas, Norma, Lozano-Elena, Fidel, Blasco-Escámez, David, Tohge, Takayuki, Martínez-Andújar, Cristina, Albacete, Alfonso, Osorio, Sonia, Bustamante, Mariana, Riechmann, José Luis, Nomura, Takahito, Yokota, Takao, Conesa, Ana, Alfocea, Francisco Pérez, Fernie, Alisdair R., and Caño-Delgado, Ana I.
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- 2018
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26. The genetic basis of fruit morphology in horticultural crops: lessons from tomato and melon
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Monforte, Antonio J., Diaz, Aurora, Caño-Delgado, Ana, and van der Knaap, Esther
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- 2014
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27. Experimental and Theoretical Methods to Approach the Study of Vascular Patterning in the Plant Shoot
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Fàbregas, Norma, primary, Formosa-Jordan, Pau, additional, Ibañes, Marta, additional, and Caño-Delgado, Ana I., additional
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- 2017
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28. The Primary Root of Sorghum bicolor (L. Moench) as a Model System to Study Brassinosteroid Signaling in Crops
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Blasco-Escámez, David, primary, Lozano-Elena, Fidel, additional, Fàbregas, Norma, additional, and Caño-Delgado, Ana I., additional
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- 2017
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29. Methods for Modeling Brassinosteroid-Mediated Signaling in Plant Development
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Frigola, David, primary, Caño-Delgado, Ana I., additional, and Ibañes, Marta, additional
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- 2017
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30. A Method for Rapid and Reliable Molecular Detection of Drought-Response Genes in Sorghum bicolor (L.) Moench Roots
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Juan B, Fontanet-Manzaneque, David, Blasco-Escámez, Damiano, Martignago, Andrés, Rico-Medina, and Ana I, Caño-Delgado
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Plant Breeding ,Gene Expression Regulation, Plant ,Stress, Physiological ,Brassinosteroids ,Arabidopsis ,Edible Grain ,Ecosystem ,Sorghum ,Droughts - Abstract
Drought is a major environmental stress that limits growth and productivity in agricultural ecosystems limiting crop yield worldwide. Breeding crops for enhanced drought tolerance is a priority to preserve food security on the increasing world population. Recent work in Arabidopsis has shown that vascular brassinosteroid receptor BRL3 (Brassinosteroid insensitive like-3) transcriptionally controls the production of osmoprotectant metabolites that confer drought resistance without penalizing growth, offering new and exciting possibilities for biotechnological improvement of drought-resistant crops. In cereals, understanding transcriptional responses to drought is an essential step for the production of gene-edited drought-resistant cereals. In this chapter, we present a method to analyze the transcriptional responses to drought in Sorghum bicolor (L.) Moench, our cereal of choice. Among the genes we tested, we found that drought marker gene SbDHN1 has a 1000-fold increase only after 1 day of drought, bringing possibilities for the development of molecular sensors for testing drought. Overall, this analysis is useful to set up conditions of high-throughput transcriptomic analysis of drought stressed plants before drought phenotype is observed.
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- 2022
31. BRAVO self-confined expression through WOX5 in the Arabidopsis root stem-cell niche
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Mercadal, Josep, primary, Betegón-Putze, Isabel, additional, Bosch, Nadja, additional, Caño-Delgado, Ana I., additional, and Ibañes, Marta, additional
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- 2022
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32. The BRASSINOSTEROID INSENSITIVE1—LIKE3 Signalosome Complex Regulates Arabidopsis Root Development
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Fàbregas, Norma, Li, Na, Boeren, Sjef, Nash, Tara E., Goshe, Michael B., Clouse, Steven D., de Vries, Sacco, and Caño-Delgado, Ana I.
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- 2013
33. Genetic and molecular study of brassinosteroid receptors in sorghum bicolor
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Caño-Delgado, Ana I., Blasco‐Escámez, David, Caño-Delgado, Ana I., and Blasco‐Escámez, David
- Abstract
[EN] Brassinosteroids have been proven to be of great importance for the development of plants but also in the adaptation of plants to different biotic and abiotic stresses. Our previous work in Arabidopsis showed that BRL3, a discretely expressed Brassinosteroid receptor, intervenes in the adaptative response of plants to drought, and BRL3 overexpressing plants were tolerant to drought. Therefore, we wanted to test whether this adaptative mechanism is conserved in one of the most important crops in arid and semiarid zones of the planet, Sorghum bicolor. In the first chapter, we identified the two Brassinosteroid receptor genes of Sorghum bicolor. Their genetic sequence and the protein 3D structure of BRI1 was analysed showing a high degree of conservation. Furthermore, we tested their functionality through heterologous complementation in Arabidopsis. In the second chapter, a mutagenized collection was screened in an effort to isolate six sorghum brassinosteroid receptor mutants. The plants were backcrossed to eliminate background mutations and to obtain segregant populations for plant phenotyping. Furthermore, different lines were subjected to drought and elevated temperature stresses, showing that both receptors control abiotic stress responses. The third chapter constitutes an effort to establish sorghum transformation protocol in our laboratory. Sorghum is known to be a recalcitrant specie for transformation. Thus, we established a collaboration with the laboratory of Prof. Ian Godwin, in Australia. The results obtained at CRAG during two years were compared with the results obtained during an international stay in QAAFI (Australia). We obtained transgenic sorghum plants, which will be a useful tool for further research. Overall, the aim of the present PhD thesis is to contribute to the translational research from molecular biology to agriculture. Towards this direction, we managed to set Sorghum research at CRAG, adapt protocols from Arabidopsis to Sorghum, and, [ES] Los brasinosteroides son fitothormonas esenciales para el desarrollo de las plantas y su adaptación a diferentes tipos de estrés ambiental. Nuestro trabajo previo en Arabidopsis demostró que BRL3, uno de los receptores de brasinosteroides, interviene en la respuesta adaptativa de estas plantas a la sequía. Las plantas sobreexpresoras de BRL3 toleran mejor la sequía sin penalizar el rendimiento. Por lo tanto, queríamos comprobar si este mecanismo de adaptación está conservado en uno de los cultivos más importantes en las zonas áridas y semiáridas del planeta, el Sorghum bicolor. En el primer capítulo, identificamos los dos genes receptores de brasinosteroides en el genoma de Sorghum bicolor. Su secuencia genética y la estructura proteica 3D de BRI1 fue analizada mostrando un alto grado de conservación. Además, comprobamos que son funcionales mediante complementación heteróloga en Arabidopsis. En el segundo capítulo, se identificaron varios alelos mutantes en los receptores de brasinosteroides a partir de una colección existente de mutantes. Las plantas fueron retrocruzadas dos veces, para eliminar mutaciones en el fondo genético y así obtener poblaciones segregantes para el fenotipado de las plantas. Asimismo, diferentes de estas líneas fueron sometidas a caracterización fisiológica, en ensayos de estrés por sequía y a temperaturas elevadas. En el tercer capítulo se reportan los avances hacia el establecimiento de un protocolo de transformación de sorgo en nuestro laboratorio. El sorgo es conocido como una especie recalcitrante a transformación. Así pues, establecimos una colaboración con el laboratorio del Prof. Ian Godwin, en Australia. Los resultados obtenidos en el CRAG durante dos años diferentes fueron comparados con los resultados obtenidos durante mi estancia internacional en QAAFI (Australia). Obtuvimos plantas transgénicas de sorgo que serán útiles para investigaciones futuras. En general, el objetivo de esta tesis doctoral es contribuir a la biología, [CA] La importància dels brasinosteroides per al desenvolupament de les plantes i la seva adaptació a diferents tipus d'estrès, biòtic i abiòtic, ha estat demostrada. El nostre treball previ amb Arabidopsis va demostrar que BRL3, un dels receptors de brasinosteroides discretament expressat, intervé en la resposta adaptativa d'aquestes plantes a sequera, i plantes sobreexpressores de BRL3 toleren millor la sequera. Per tant, volíem comprovar si aquest mecanisme d'adaptació està conservat en un dels cultius més importants a les zones àrides i semiàrides del planeta, Sorghum bicolor. Al primer capítol, identifiquem els dos gens receptors de brasinosteroides a Sorghum bicolor. La seqüència genètica i l'estructura proteica 3D de BRI1 va ser analitzada mostrant un alt grau de conservació. A més, vam comprobar que són funcionals mitjançant complementació heteròloga a Arabidopsis. Al segon capítol, una col·lecció mutagenitzada va ser cribada amb l'objectiu d'aïllar sis mutants de receptors de brasinosteroides. Les plantes van ser retrocreuades per eliminar mutacions en el fons genètic i així obtenir poblacions segregants per al fenotipat de les plantes. Així mateix, diferents d'aquestes línies van ser sotmeses a estrès per sequera ia temperatures elevades. El tercer capítol es reporten els esforços per establir el protocol de transformació de sorgo al nostre laboratori. El sorgo és conegut com una espècie recalcitrant a transformació. Així doncs, vam establir una col·laboració amb el laboratori del Prof. Ian Godwin, a Austràlia. Els resultats obtinguts al CRAG durant dos anys diferents van ser comparats amb els resultats obtinguts durant una estada internacional a QAAFI (Austràlia). Vam obtenir plantes transgèniques de sorgo que seran útils per a altres investigacions. En general, l'objectiu d'aquesta tesi doctoral és contribuir a la recerca translativa de biologia molecular cap a l'agricultura. En aquesta direcció, aconseguim establir la investigació sobre sorgo al CRAG, a
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- 2022
34. Brassinosteroid-mediated stem cell divisions in response to DNA damage in Arabidopsis thaliana
- Author
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Caño-Delgado, Ana I., Planas-Riverola, Ainoa, Caño-Delgado, Ana I., and Planas-Riverola, Ainoa
- Abstract
[EN] The present PhD thesis dissertation reports novel mechanisms for stem cell divisions mediated by brassinosteroids (BRs) upon DNA damage conditions in plants. BRs are steroid hormones involved in multiple process of plant growth and development, and the adaptation to the environment. One of the processes that are known to be regulated by BRs is the stem cell divisions. Plants stem cells are in niches, which are mainly found in the root and shoot apexes. At the core of the root stem cell niche it is placed the quiescent center (QC), a group of cells with very low mitotic activity that maintain the undifferentiated status of surrounding stem cells and act as a cell reservoir. QC cells only trigger their divisions when need to replenish the stem cells, for example, after a DNA damage. BR signaling is in charge of triggering these QC divisions, but the exact mechanisms of how this process is regulated is still unknown. Here, we use an interdisciplinary approach, using Arabidopsis thaliana as a model system, including molecular genetics, physiology and bioinformatics to decipher the role of BR receptors upon DNA damage regulating the QC divisions. The results presented in this thesis dissertation uncover novel roles for the BR-receptor kinase BRL3 (BRI1-like 3) protein in DNA damage and DNA repair machinery in plants. This is important in order to control DNA repair mechanisms and cell cycle progression in the root meristem, which requires a balance of these processes to ensure plant adaptation to adverse conditions. We found that, at the root apex, BRL3 downstream signaling events modulate an enzyme that is specifically expressed in the QC cells, the RNR2A (RIBONUCLEOTIDE REDUCTASE 2A), in charge of maintaining dNTPs (deoxynucleotide triphosphates) supply during DNA synthesis. Overall, we found that RNR2A is crucial for a proper QC division in response to DNA damage conditions. Moreover, we also discovered that BRL3 pathway is also involved in the triggering of ROS, [ES] La presente tesis doctoral describe nuevos mecanismos mediados por brasinoesteroides (BRs) para controlar las divisiones de las células madre en condiciones de daño en el ADN en las plantas. Los BRs son hormonas esteroideas involucradas en múltiples procesos de crecimiento y de desarrollo en las plantas, y en la adaptación al medio ambiente. Se sabe que la división de las células madre está regulada por BRs. Las células madre de las plantas se encuentran en nichos, que se localizan principalmente en los ápices de las raíces y de los tallos. En el centro del nicho de células madre de la raíz se encuentra el centro quiescente (QC), un grupo de células con muy baja actividad mitótica, que mantiene el estado indiferenciado de las células madre circundantes y actúa como un reservorio celular. Las células del QC solo se dividen cuando necesitan reponer las células madre, por ejemplo, después de un daño en el ADN. Las señales mediadas por BR son las encargadas de activar estas divisiones del QC pero aún se desconocen los mecanismos exactos de cómo se regula este proceso. En este trabajo, utilizamos un enfoque interdisciplinario, utilizando Arabidopsis thaliana como sistema modelo, que incluye genética molecular, fisiología y bioinformática, para descifrar el papel de los receptores de los BRs en respuesta al daño al ADN y en la regulación de las divisiones del QC. Los resultados presentados en esta tesis descubren nuevos roles para la proteína quinasa BRL3 (BRI1-like 3) en el daño al ADN y la maquinaria de reparación del ADN en las plantas. Esto es importante para controlar los mecanismos de reparación del ADN y la progresión en el ciclo celular del meristemo de la raíz, lo que requiere un equilibrio de estos procesos para asegurar la adaptación de la planta a condiciones adversas. Encontramos que, en el ápice de la raíz, los eventos de señalización aguas abajo de BRL3 modulan una enzima que se expresa específicamente en las células del QC, la RNR2A (RIBONUCLEOTIDE RE, [CA] La present tesi doctoral descriu nous mecanismes regulats per brassinosteroides (BRs) per controlar les divisions de les cèl·lules mare en condicions de dany al DNA a les plantes. Les BRs són hormones esteroides implicades en múltiples processos de creixement i de desenvolupament de les plantes, i en l'adaptació al medi ambient. Se sap que les divisions de les cèl·lules mare estan regulades per BRs. Les cèl·lules mare de les plantes es troben en nínxols, que es troben principalment als àpexs de les arrels i de les tiges. Al centre del nínxol de les cèl·lules mare de l'arrel està el centre quiescent (QC), un grup de cèl·lules amb una activitat mitòtica molt baixa que manté l'estat indiferenciat de les cèl·lules mare circumdants i actua com a reservori cel·lular. Les cèl·lules del QC només activen les seves divisions quan necessiten reemplenar les cèl·lules mare, per exemple, després d'un dany al DNA. La senyalització per BRs s'encarrega d'activar aquestes divisions del QC, però encara es desconeixen els mecanismes exactes de com es regula aquest procés. En aquest treball, utilitzem un enfocament interdisciplinari, utilitzant Arabidopsis thaliana com a sistema model, que inclou genètica molecular, fisiologia i bioinformàtica, per desxifrar el paper dels receptors de BRs en resposta al dany al DNA i en la regulació de les divisions del QC. Els resultats presentats en aquesta tesi descobreixen noves funcions per a la proteïna quinasa BRL3 (BRI1-like 3) en el dany al DNA i en la maquinària de reparació de l'ADN a les plantes. Això és important per controlar els mecanismes de reparació del DNA i la progressió del cicle cel·lular al meristema de l'arrel, que requereix un equilibri d'aquests processos per garantir l'adaptació de la planta a condicions adverses. Vam trobar que, a l'àpex de l'arrel, els esdeveniments de senyalització aigües avall de BRL3 modulen un enzim que s'expressa específicament a les cèl·lules del QC, la RNR2A (RIBONUCLEOTIDE REDUCTASE 2A), encarre
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- 2022
35. A method for rapid and reliable molecular detection of drought-response genes in Sorghum bicolor (L.) Moench roots
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Fontanet-Manzaneque, Juan B., Blasco‐Escámez, David, Martignago, Damiano, Rico Medina, Andrés, Caño-Delgado, Ana I., Fontanet-Manzaneque, Juan B., Blasco‐Escámez, David, Martignago, Damiano, Rico Medina, Andrés, and Caño-Delgado, Ana I.
- Abstract
Drought is a major environmental stress that limits growth and productivity in agricultural ecosystems limiting crop yield worldwide. Breeding crops for enhanced drought tolerance is a priority to preserve food security on the increasing world population. Recent work in Arabidopsis has shown that vascular brassinosteroid receptor BRL3 (Brassinosteroid insensitive like-3) transcriptionally controls the production of osmoprotectant metabolites that confer drought resistance without penalizing growth, offering new and exciting possibilities for biotechnological improvement of drought-resistant crops. In cereals, understanding transcriptional responses to drought is an essential step for the production of gene-edited drought-resistant cereals. In this chapter, we present a method to analyze the transcriptional responses to drought in Sorghum bicolor (L.) Moench, our cereal of choice. Among the genes we tested, we found that drought marker gene SbDHN1 has a 1000-fold increase only after 1 day of drought, bringing possibilities for the development of molecular sensors for testing drought. Overall, this analysis is useful to set up conditions of high-throughput transcriptomic analysis of drought stressed plants before drought phenotype is observed.
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- 2022
36. Analysis of metabolic dynamics during drought stress in Arabidopsis plants
- Author
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European Commission, Ministerio de Economía y Competitividad (España), EMBO, Fundación Renta Corporación, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Generalitat de Catalunya, Lozano-Elena, Fidel, Fàbregas Vallvé, Norma, Coleto, Veredas, Caño-Delgado, Ana I., European Commission, Ministerio de Economía y Competitividad (España), EMBO, Fundación Renta Corporación, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Generalitat de Catalunya, Lozano-Elena, Fidel, Fàbregas Vallvé, Norma, Coleto, Veredas, and Caño-Delgado, Ana I.
- Abstract
Drought is a major cause of agricultural losses worldwide. Climate change will intensify drought episodes threatening agricultural sustainability. Gaining insights into drought response mechanisms is vital for crop adaptation to climate emergency. To date, only few studies report comprehensive analyses of plant metabolic adaptation to drought. Here, we present a multifactorial metabolomic study of early-mid drought stages in the model plant Arabidopsis thaliana. We sampled root and shoot tissues of plants subjected to water withholding over a six-day time course, including brassinosteroids receptor mutants previously reported to show drought tolerance phenotypes. Furthermore, we sequenced the root transcriptome at basal and after 5 days drought, allowing direct correlation between metabolic and transcriptomic changes and the multi-omics integration. Significant abiotic stress signatures were already activated at basal conditions in a vascular-specific receptor overexpression (BRL3ox). These were also rapidly mobilized under drought, revealing a systemic adaptation strategy driven from inner tissues of the plant. Overall, this dataset provides a significant asset to study drought metabolic adaptation and allows its analysis from multiple perspectives.
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- 2022
37. BRAVO self-confined expression through WOX5 in the Arabidopsis root stem-cell niche
- Author
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European Commission, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Economía y Competitividad (España), European Research Council, Generalitat de Catalunya, Mercadal, Josep, Betegón-Putze, Isabel, Bosch, Nadja, Caño-Delgado, Ana I., Ibañes, Marta, European Commission, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Economía y Competitividad (España), European Research Council, Generalitat de Catalunya, Mercadal, Josep, Betegón-Putze, Isabel, Bosch, Nadja, Caño-Delgado, Ana I., and Ibañes, Marta
- Abstract
In animals and plants, stem-cell niches are local microenvironments that are tightly regulated to preserve their unique identity while communicating with adjacent cells that will give rise to specialized cell types. In the primary root of Arabidopsis thaliana, two transcription factors, BRAVO and WOX5, among others, are expressed in the stem-cell niche. Intriguingly, BRAVO, a repressor of quiescent center divisions, confines its own gene expression to the stem-cell niche, as evidenced in a bravo mutant background. Here, we propose through mathematical modeling that BRAVO confines its own expression domain to the stem-cell niche by attenuating a WOX5-dependent diffusible activator of BRAVO. This negative feedback drives WOX5 activity to be spatially restricted as well. The results show that WOX5 diffusion and sequestration by binding to BRAVO are sufficient to drive the experimentally observed confined BRAVO expression at the stem-cell niche. We propose that the attenuation of a diffusible activator can be a general mechanism acting at other stem-cell niches to spatially confine genetic activity to a small region while maintaining signaling within them and with the surrounding cells.
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- 2022
38. Tackling Drought Stress: RECEPTOR-LIKE KINASES Present New Approaches
- Author
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Marshall, Alex, Aalen, Reidunn B., Audenaert, Dominique, Beeckman, Tom, Broadley, Martin R., Butenko, Melinka A., Caño-Delgado, Ana I., de Vries, Sacco, Dresselhaus, Thomas, Felix, Georg, Graham, Neil S., Foulkes, John, Granier, Christine, Greb, Thomas, Grossniklaus, Ueli, Hammond, John P., Heidstra, Renze, Hodgman, Charlie, Hothorn, Michael, Inzé, Dirk, Østergaard, Lars, Russinova, Eugenia, Simon, Rüdiger, Skirycz, Aleksandra, Stahl, Yvonne, Zipfel, Cyril, and De Smet, Ive
- Published
- 2012
39. BRAVO self-confined expression through WOX5 in the Arabidopsis root stem-cell niche
- Author
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Josep Mercadal, Isabel Betegón-Putze, Nadja Bosch, Ana I. Caño-Delgado, Marta Ibañes, European Commission, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Economía y Competitividad (España), European Research Council, and Generalitat de Catalunya
- Subjects
Homeodomain Proteins ,WOX5 ,Arabidopsis Proteins ,Meristem ,Arabidopsis ,Sequestration ,Plant Roots ,Diffusion ,Root ,Gene Expression Regulation, Plant ,Nitriles ,BRAVO ,Stem Cell Niche ,Molecular Biology ,Stem-cell niche ,Developmental Biology - Abstract
In animals and plants, stem-cell niches are local microenvironments that are tightly regulated to preserve their unique identity while communicating with adjacent cells that will give rise to specialized cell types. In the primary root of Arabidopsis thaliana, two transcription factors, BRAVO and WOX5, among others, are expressed in the stem-cell niche. Intriguingly, BRAVO, a repressor of quiescent center divisions, confines its own gene expression to the stem-cell niche, as evidenced in a bravo mutant background. Here, we propose through mathematical modeling that BRAVO confines its own expression domain to the stem-cell niche by attenuating a WOX5-dependent diffusible activator of BRAVO. This negative feedback drives WOX5 activity to be spatially restricted as well. The results show that WOX5 diffusion and sequestration by binding to BRAVO are sufficient to drive the experimentally observed confined BRAVO expression at the stem-cell niche. We propose that the attenuation of a diffusible activator can be a general mechanism acting at other stem-cell niches to spatially confine genetic activity to a small region while maintaining signaling within them and with the surrounding cells., This work was supported by the Ministerio de Ciencia e Innovación (MCIN), Agencia Estatal de Investigación (AEI) and European Regional Development Fund (Fondo Europeo de Desarrollo Regional, FEDER) program ‘Una manera de hacer Europa’ MCIN/AEI/10.13039/501100011033/FEDER (PGC2018-101896-B-I00 to M.I.); Ministerio de Ciencia e Innovación, Agencia Estatal de Investigación and European Social Fund (Fondo Social Europeo, FSE) program ‘El FSE invierte en tu futuro’ (BES-2016-078218 to J.M.); Ministerio de Ciencia e Innovación (MCIN), Agencia Estatal de Investigación (AEI) and European Regional Development Fund (ERDF) (BIO2016-78955 to A.I.C.-D.); the European Research Council (ERC) Consolidator Grant (ERC-2015-CoG–683163 to A.I.C.-D.); Generalitat de Catalunya (M.I. and J.M. in Grup de Recerca Consolidat 2017 SGR 1061; FI-DGR 2016FI-B 00472, also funded by FSE, to N.B.); and the Ministerio de Educación, Cultura y Deporte (FPU15/02822 to I.B.-P.).
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- 2022
40. A Method for Rapid and Reliable Molecular Detection of Drought-Response Genes in Sorghum bicolor (L.) Moench Roots
- Author
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Juan B. Fontanet-Manzaneque, David Blasco-Escámez, Damiano Martignago, Andrés Rico-Medina, and Ana I. Caño-Delgado
- Subjects
Drought ,Drought marker genes ,Root ,Sorghum ,qRT-PCR ,Brassinosteroids ,Droughts ,Ecosystem ,Edible Grain ,Gene Expression Regulation, Plant ,Plant Breeding ,Stress, Physiological ,Arabidopsis ,Physiological ,Settore BIO/11 - Biologia Molecolare ,Plant ,Stress ,Gene Expression Regulation ,Settore BIO/04 - Fisiologia Vegetale - Published
- 2022
41. Self-confined expression in the Arabidopsis root stem cell niche
- Author
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Josep Mercadal, Isabel Betegón-Putze, Nadja Bosch, Ana I. Caño-Delgado, and Marta Ibañes
- Abstract
Stem cell niches are local microenvironments that preserve their unique identity while communicating with adjacent tissues. In the primary root of Arabidopsis thaliana, the stem cell niche comprises the expression of two transcription factors, BRAVO and WOX5, among others. Intriguingly, these proteins confine their own gene expression to the niche, as evidenced in each mutant background. Here we propose through mathematical modeling that BRAVO confines its own expression domain to the stem cell niche by attenuating its WOX5-dependent diffusible activator. This negative feedback drives WOX5 action to be spatially restricted as well. The results show that WOX5 diffusion and sequestration by binding to BRAVO is sufficient to drive realistic confined BRAVO expression at the stem cell niche. We propose that attenuation of a diffusible activator can be a general mechanism to confine genetic activity to a small region while at the same time maintain signaling within it and with the surrounding cells.
- Published
- 2021
42. Brassinosteroid Signaling and Auxin Transport Are Required to Establish the Periodic Pattern of Arabidopsis Shoot Vascular Bundles
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Ibañes, Marta, Fàbregas, Norma, Chory, Joanne, and Caño-Delgado, Ana I.
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- 2009
- Full Text
- View/download PDF
43. Tomato BRASSINOSTEROID INSENSITIVE1 Is Required for Systemin-Induced Root Elongation in Solanum pimpinellifolium but Is Not Essential for Wound Signaling
- Author
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Holton, Nicholas, Caño-Delgado, Ana, Harrison, Kate, Montoya, Teresa, Chory, Joanne, and Bishop, Gerard J.
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- 2007
- Full Text
- View/download PDF
44. Self-confined expression in the Arabidopsis root stem cell niche
- Author
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Mercadal, Josep, primary, Betegón-Putze, Isabel, additional, Bosch, Nadja, additional, Caño-Delgado, Ana I., additional, and Ibañes, Marta, additional
- Published
- 2021
- Full Text
- View/download PDF
45. The BES1/BZR1-family transcription factor MpBES1 regulates cell division and differentiation in Marchantia polymorpha
- Author
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Mecchia, Martin A., primary, García-Hourquet, Mariano, additional, Lozano-Elena, Fidel, additional, Planas-Riverola, Ainoa, additional, Blasco-Escamez, David, additional, Marquès-Bueno, Mar, additional, Mora-García, Santiago, additional, and Caño-Delgado, Ana I., additional
- Published
- 2021
- Full Text
- View/download PDF
46. PloidyQuantX: A Quantitative Microscopy Imaging Tool for Ploidy Quantification at Cell and Organ Level in Arabidopsis Root
- Author
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Sevillano, Xavier, primary, Ferrer, Marc, additional, González-García, Mary-Paz, additional, Pavelescu, Irina, additional, and Caño-Delgado, Ana I., additional
- Published
- 2015
- Full Text
- View/download PDF
47. Analysis of metabolic dynamics during drought stress in Arabidopsis plants
- Author
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Fidel Lozano-Elena, Norma Fàbregas, Veredas Coleto-Alcudia, and Ana I. Caño-Delgado
- Subjects
Statistics and Probability ,Arabidopsis Proteins ,fungi ,Arabidopsis ,food and beverages ,Molecular engineering in plants ,Library and Information Sciences ,Adaptation, Physiological ,Computer Science Applications ,Education ,Droughts ,Gene Expression Regulation, Plant ,Stress, Physiological ,Metabolomics ,Statistics, Probability and Uncertainty ,Transcriptomics ,Information Systems - Abstract
Drought is a major cause of agricultural losses worldwide. Climate change will intensify drought episodes threatening agricultural sustainability. Gaining insights into drought response mechanisms is vital for crop adaptation to climate emergency. To date, only few studies report comprehensive analyses of plant metabolic adaptation to drought. Here, we present a multifactorial metabolomic study of early-mid drought stages in the model plant Arabidopsis thaliana. We sampled root and shoot tissues of plants subjected to water withholding over a six-day time course, including brassinosteroids receptor mutants previously reported to show drought tolerance phenotypes. Furthermore, we sequenced the root transcriptome at basal and after 5 days drought, allowing direct correlation between metabolic and transcriptomic changes and the multi-omics integration. Significant abiotic stress signatures were already activated at basal conditions in a vascular-specific receptor overexpression (BRL3ox). These were also rapidly mobilized under drought, revealing a systemic adaptation strategy driven from inner tissues of the plant. Overall, this dataset provides a significant asset to study drought metabolic adaptation and allows its analysis from multiple perspectives.
- Published
- 2021
48. Precise transcriptional control of cellular quiescence by BRAVO/WOX5 complex in Arabidopsis roots
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Betegón‐Putze, Isabel, primary, Mercadal, Josep, additional, Bosch, Nadja, additional, Planas‐Riverola, Ainoa, additional, Marquès‐Bueno, Mar, additional, Vilarrasa‐Blasi, Josep, additional, Frigola, David, additional, Burkart, Rebecca C, additional, Martínez, Cristina, additional, Conesa, Ana, additional, Sozzani, Rosangela, additional, Stahl, Yvonne, additional, Prat, Salomé, additional, Ibañes, Marta, additional, and Caño‐Delgado, Ana I, additional
- Published
- 2021
- Full Text
- View/download PDF
49. TOPLESS mediates brassinosteroid control of shoot boundaries and root meristem development in Arabidopsis thaliana
- Author
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Ana I. Caño-Delgado, Ana Espinosa-Ruiz, Norma Fàbregas, Miguel de Lucas, Cristina Martínez, Nadja Bosch, Salomé Prat, Ministerio de Ciencia e Innovación (España), Ministerio de Economía y Competitividad (España), European Research Council, and European Commission
- Subjects
0301 basic medicine ,Cell division ,Organ boundary ,Organogenesis ,Quiescence ,03 medical and health sciences ,chemistry.chemical_compound ,TOPLESS ,Arabidopsis ,Gene expression ,Botany ,Brassinosteroid ,Molecular Biology ,Transcription factor ,biology ,Meristem ,biology.organism_classification ,Cell biology ,030104 developmental biology ,chemistry ,Quiescent center ,EAR domain ,Ectopic expression ,BES1 ,BR signaling ,Developmental Biology - Abstract
The transcription factor BRI1-EMS-SUPRESSOR 1 (BES1) is a master regulator of brassinosteroid (BR)-regulated gene expression. BES1 together with BRASSINAZOLE-RESISTANT 1 (BZR1) drive activated or repressed expression of several genes, and have a prominent role in negative regulation of BR synthesis. Here, we report that BES1 interaction with TOPLESS (TPL), via its ERF-associated amphiphilic repression (EAR) motif, is essential for BES1-mediated control of organ boundary formation in the shoot apical meristem and the regulation of quiescent center (QC) cell division in roots. We show that TPL binds via BES1 to the promoters of the CUC3 and BRAVO targets and suppresses their expression. Ectopic expression of TPL leads to similar organ boundary defects and alterations in QC cell division rate to the bes1-d mutation, while bes1-d defects are suppressed by the dominant interfering protein encoded by tpl-1, with these effects respectively correlating with changes in CUC3 and BRAVO expression. Together, our data unveil a pivotal role of the co-repressor TPL in the shoot and root meristems, which relies on its interaction with BES1 and regulation of BES1 target gene expression., C.M. was initially supported by a Juan de la Cierva contract from the Spanish Ministry of Science and Innovation (Ministerio de Ciencia e Innovación). This work was supported by grants BIO2011-30546 and BIO2014-60064-R from the Spanish Ministry of Economy and Competitiveness (Ministerio de Economía y Competitividad, MINECO). The A.I.C.-D. laboratory is funded by a BIO2013-43873 grant from MINECO and by a European Research Council Consolidator Grant (ERC-2015-CoG-683163).
- Published
- 2021
50. Auxin-regulated reversible inhibition of TMK1 signaling by MAKR2 modulates the dynamics of root gravitropism
- Author
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Lise C. Noack, Tom Beeckman, Jiří Friml, Laia Armengot, Zachary L. Nimchuk, Ana I. Caño-Delgado, Maria Mar Marquès-Bueno, Barbara K. Möller, Matthieu Pierre Platre, Davy Opdenacker, Vincent Bayle, Mengying Liu, Steffen Vanneste, Lesia Rodriguez, Yvon Jaillais, Joseph Bareille, European Research Council, European Commission, Agence Nationale de la Recherche (France), Ministerio de Economía y Competitividad (España), National Science Foundation (US), Research Foundation - Flanders, Reproduction et développement des plantes (RDP), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institute of Science and Technology [Austria] (IST Austria), Vlaams Instituut voor Biotechnologie [Ghent, Belgique] (VIB), University of North Carolina [Chapel Hill] (UNC), University of North Carolina System (UNC), Centre for Research in Agricultural Genomics (CRAG), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), and Institute of Science and Technology [Klosterneuburg, Austria] (IST Austria)
- Subjects
0301 basic medicine ,EFFLUX ,[SDV]Life Sciences [q-bio] ,PROTEIN ,Plant Roots ,chemistry.chemical_compound ,0302 clinical medicine ,Loss of Function Mutation ,Arabidopsis ,CELL-SURFACE ,Brassinosteroid ,Auxin ,Receptor-like kinase ,chemistry.chemical_classification ,Anionic lipids ,biology ,food and beverages ,Plants, Genetically Modified ,ARABIDOPSIS ,Transmembrane protein ,gravitropism ,Cell biology ,receptor-like kinase ,Gain of Function Mutation ,INTRACELLULAR TRAFFICKING ,Plant hormone ,Signal transduction ,ABP1 ,General Agricultural and Biological Sciences ,Gravitation ,Signal Transduction ,EXPRESSION ,ENDOCYTOSIS ,Gravitropism ,Protein Serine-Threonine Kinases ,anionic lipids ,Endocytosis ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,RHO ,ROP6 ,Report ,Indoleacetic Acids ,Arabidopsis Proteins ,fungi ,Membrane Proteins ,Biology and Life Sciences ,RECEPTOR-LIKE KINASES ,biology.organism_classification ,root ,TMK ,MAKR ,030104 developmental biology ,chemistry ,Root ,brassinosteroid ,auxin ,030217 neurology & neurosurgery - Abstract
Summary Plants are able to orient their growth according to gravity, which ultimately controls both shoot and root architecture.1 Gravitropism is a dynamic process whereby gravistimulation induces the asymmetric distribution of the plant hormone auxin, leading to asymmetric growth, organ bending, and subsequent reset of auxin distribution back to the original pre-gravistimulation situation.1, 2, 3 Differential auxin accumulation during the gravitropic response depends on the activity of polarly localized PIN-FORMED (PIN) auxin-efflux carriers.1, 2, 3, 4 In particular, the timing of this dynamic response is regulated by PIN2,5,6 but the underlying molecular mechanisms are poorly understood. Here, we show that MEMBRANE ASSOCIATED KINASE REGULATOR2 (MAKR2) controls the pace of the root gravitropic response. We found that MAKR2 is required for the PIN2 asymmetry during gravitropism by acting as a negative regulator of the cell-surface signaling mediated by the receptor-like kinase TRANSMEMBRANE KINASE1 (TMK1).2,7, 8, 9, 10 Furthermore, we show that the MAKR2 inhibitory effect on TMK1 signaling is antagonized by auxin itself, which triggers rapid MAKR2 membrane dissociation in a TMK1-dependent manner. Our findings suggest that the timing of the root gravitropic response is orchestrated by the reversible inhibition of the TMK1 signaling pathway at the cell surface., Graphical Abstract, Highlights • MAKR2 is co-expressed with PIN2 and regulates the pace of root gravitropism • MAKR2 controls PIN2 asymmetric accumulation at the root level during gravitropism • MAKR2 binds to and is a negative regulator of the TMK1 receptor kinase • Auxin antagonizes the MAKR2 inhibition of TMK1 by delocalizing MAKR2 in the cytosol, Marquès-Bueno, Armengot et al. show that the unstructured protein MAKR2 controls the dynamics of the root gravitropic response by acting as a negative regulator of the TMK1 receptor kinase. In addition, the MAKR2 inhibitory effect on TMK1 signaling is antagonized by auxin itself, which triggers rapid MAKR2 membrane dissociation in a TMK1-dependent manner.
- Published
- 2021
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