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Background: Although transradial access (TRA) has become the main vascular access for coronary intervention, its high radial artery occlusion rate limits its application in some patients. Studies have shown that compared with TRA, distal transradial access (dTRA) with the snuffbox area or the Hegu acupoint area as the puncture point significantly decreases the incidence of radial artery occlusion. However, no randomized controlled study has confirmed the safety and efficacy of coronary artery intervention via dTRA in China. Methods and analyses: This single-center, prospective, randomized controlled, superiority open-label study will enroll 428 consecutive patients with coronary heart disease undergoing percutaneous coronary intervention as the study population. After preoperative evaluation, the participants will be randomly divided into a study group (dTRA) and control group (TRA) in a 1:1 ratio. The primary endpoint (radial artery occlusion at 24 hours after operation) and secondary endpoint events will be evaluated and recorded. Study registration: This study has been registered in the Chinese Clinical Trial Registry (registration number: ChiCTR2300073902).

Objective: This study aimed to study the changes of active components in irradiated Rhodiola rosea extracts （RRE） and their hypoglycemic effect. Methods: Rhodiola rosea was irradiated with 60Co-γ rays at doses of 5, 10, 20 and 30 kGy, respectively. High performance liquid chromatography （HPLC） was used to analyze RRE compounds before （0 kGy） and after irradiation. The type 1 diabetic mouse models established by intraperitoneal injection of Streptozotocin （STZ） were given a gavage. The body weight, fasting blood glucose, food intake and water intake of mice were measured regularly. Oral glucose tolerance was measured on the last day, after dissection, organ coefficient, serum total cholesterol （TC）, glycosylated hemoglobin （GHb）, total triglyceride （TG）, malondialdehyde （MDA）, superoxide dismutase （SOD） and catalase （CAT） were measured. Results: The content of salidroside in RRE was significantly decreased after irradiation （P＜0.05）, and the content of loxavir was significantly increased at 5, 10 and 20 kGy （P＜0.05）. Tyrosol was detected at 20 kGy. Compared with the model group, the body weight of mice was given a gavage with irradiation RRE was increased, the food intake, water intake and blood glucose level decreased, and the food utilization rate increased significantly （P＜0.05）. The levels of TC, GHb, TG and MDA were significantly decreased （P＜0.05）, while the activities of SOD and CAT were significantly increased （P＜0.05）. The phenomenon of organ swelling was improved, and the effect was better at 20 kGy, and there was a significant difference with other irradiation doses （P＜0.05）. Conclusion: The composition and content of RRE compounds changed after irradiation, and RRE can reduce the level of diabetes in type 1 diabetic mice before and after irradiation. The irradiation dose of 20 kGy is effective.

Objective: To explore the effects of organic acids on tobacco pH and flue gas pH. Methods: 34 kinds of single-grade tobacco and 11 kinds of finished cigarettes were taken as the research objects, and their non-volatile organic acids, tobacco pH, and the pH of mainstream smoke total particulate matter were measured respectively. Grey correlation analysis and principal component analysis were used to analyze the correlation between organic acids and pH. Results: The results of grey correlation analysis show that oxalic acid, palmitic acid, malic acid and oleic acid have a relatively large influence on the pH of single-grade tobacco; Palmitic acid, malic acid, linolenic acid and oxalic acid have a relatively large impact on the pH of cigarette cut tobacco; Malic acid, palmitic acid, linolenic acid and oxalic acid have relatively large effects on the pH of mainstream smoke total particulate matter of the cigarettes. Conclusion: Comprehensive analysis shows that malic acid and oxalic acid have a greater influence on the pH of single-grade tobacco, and malic acid and palmitic acid have a greater influence on the pH of cigarette cut tobacco and the pH of mainstream smoke total particulate matter.

ObjectiveUsing meta-analysis, we evaluate circulating tumor cells(CTCs) as a potential diagnostic tool for breast cancer.MethodsA document search was conducted using publicly available databases up to May 2021. Specific inclusion and exclusion criteria were formulated and summarize relevant data through literature types, research types, case populations, samples, etc. Subgroup analysis of documents based on regions, enrichment methods, and detection methods. The included research projects were evaluated using DeeKs’ bias, and evaluation indicators such as specificity (SPE), sensitivity (SEN), diagnosis odds ratio (DOR) were used as evaluation indicators.Results16 studies on the use of circulating tumor cells to diagnose breast cancer were included in our meta-analysis. Overall sensitivity value was 0.50 (95%CI:0.48-0.52), specificity value was 0.93 (95%CI:0.92- 0.95), DOR value was 33.41 (95%CI:12.47-89.51), and AUC value was 0.8129.ConclusionIn meta-regressions and subgroup analysis, potential heterogeneity factors were analyzed, but the source of heterogeneity is still unclear. CTCs, as a novel tumor marker, have a good diagnostic value, but its enrichment and detection methods still need to continue to be developed to improve detection accuracy. Therefore, CTCs can be used as an auxiliary means of early detection, which is helpful to the diagnosis and screening of breast cancer.

Due to the nonstationarity, complex transmission path, high system noise and serious modulation phenomenon of wind turbine gearbox vibration signal, the conventional spectrum analysis method was difficult to identify the fault location of gearbox. In order to solve the above problems, a fault diagnosis method of wind turbine planetary gearbox based on synchronous extraction（SET）and vold-Kalman filter（SEV） was proposed in this paper. This paper first describes the principle and process of the proposed method. The vibration signal of the generator bearing was extracted by the local energy maximum method based on SET（LMS）, and then the instantaneous frequency was accurately extracted based on the ward Kaman filter to obtain the complete phase information. Finally, the detailed fault location information of wind turbine gearbox was obtained by order analysis. Simulation and experimental analysis verify the effectiveness of the proposed method.

Most anticancer treatments only induce the death of ordinary cancer cells, while cancer stem cells (CSCs) in the quiescent phase of cell division are difficult to kill, which eventually leads to cancer drug resistance, metastasis, and relapse. Therefore, CSCs are also important in targeted cancer therapy. Herein, we developed dual-targeted and glutathione (GSH)-responsive novel nanoparticles (SSBPEI–DOX@siRNAs/iRGD–PEG–HA) to efficiently and specifically deliver both doxorubicin and small interfering RNA cocktails (siRNAs) (survivin siRNA, Bcl-2 siRNA and ABCG2 siRNA) to ovarian CSCs. They are fabricated via electrostatic assembly of anionic siRNAs and cationic disulfide bond crosslinking-branched polyethyleneimine-doxorubicin (SSBPEI–DOX) as a core. Interestingly, the SSBPEI–DOX could be degraded into low-cytotoxic polyethyleneimine (PEI). Because of the enrichment of glutathione reductase in the tumor microenvironment, the disulfide bond (–SS–) in SSBPEI–DOX can be specifically reduced to promote the controlled release of siRNA and doxorubicin (DOX) in the CSCs. siRNA cocktails could specifically silence three key genes in CSCs, which, in combination with the traditional chemotherapy drug DOX, induces apoptosis or necrosis of CSCs. iRGD peptides and “sheddable” hyaluronic acid (HA) wrapped around the core could mediate CSC targeting by binding with neuropilin-1 (NRP1) and CD44 to enhance delivery. In summary, the multifunctional delivery system SSBPEI–DOX@siRNAs/iRGD–PEG–HA nanoparticles displays excellent biocompatibility, accurate CSC-targeting ability, and powerful anti-CSC ability, which demonstrates its potential value in future treatments to overcome ovarian cancer metastasis and relapse. To support this work, as exhaustive search was conducted for the literature on nanoparticle drug delivery research conducted in the last 17 years (2007–2023) using PubMed, Web of Science, and Google Scholar.

Objective: This study focused on screening lactic acid bacteria strains with potential probiotic properties. Methods: Five strains of acid-tolerant lactic acid bacteria were screened from twelve low-salt pickles with salt content ≤6%, identified by 16S rDNA, and their probiotic properties were studied. Results: The five strains were Leuconostoc mesenteroides J2, Lactobacillus paracasei N6, Lactobacillu paraplantarum F8, Weissella cibaria M1 and Weissella cibaria M12. Among them, the relative growth rates of L. paracasei N6, W. cibaria M1 and W. cibaria M12 in pH 4.0 medium were 84.23%, 78.32% and 76.29%, respectively, which had strong acid-tolerant capacity. After digestion with gastric fluid at pH 3.0 and intestinal fluid at pH 8.0, the survival rates were 79.26%, 50.65% and 53.37%, respectively, which had strong tolerance to both artificial gastric fluid and intestinal fluid environments. After cultured in 0.3% bile salt for 8 h, the survival rates were more than 30%, and they had strong bile salt tolerance. The hydrophobic rates and self-aggregation rates were both above 60%, and they had strong hydrophobic and self-aggregating capacity. Conclusion: In this study, three strains of acid-tolerant lactic acid bacteria L. paracasei N6, W. cibaria M1 and W. cibaria M12 with probiotic properties were screened, which could be used in the study of compound probiotics.

Dengue virus (DENV) is a small envelope virus of Flaviviridae that is mainly transmitted by Aedes aegypti and Aedes albopictus. It can cause dengue fever with mild clinical symptoms or even life-threatening dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). At present, there are no specific drugs or mature vaccine products to treat DENV. microRNAs (miRNAs) are a class of important non-coding small molecular RNAs that regulate gene expression at the post-transcriptional level. It is involved in and regulates a series of important life processes, such as growth and development, cell differentiation, cell apoptosis, anti-virus, and anti-tumor. miRNAs also play important roles in interactions between host and viral genome transcriptomes. Host miRNAs can directly target the genome of the virus or regulate host factors to promote or inhibit virus replication. Understanding the expression and function of miRNAs during infection with DENV and the related signal molecules of the miRNA-mediated regulatory network will provide new insights for the development of miRNA-based therapies.

Photodynamic therapy (PDT) is a promising therapy due to its efficiency and accuracy. The photosensitizer is delivered to the target lesion and locally activated. Viral nanoparticles (VNPs) have been explored as delivery vehicles for PDT in recent years because of their favorable properties, including simple manufacture and good safety profile. They have great potential as drug delivery carriers in medicine. Here, we review the development of PDT photosensitizers and discuss applications of VNP-mediated photodynamic therapies and the performance of VNPs in the treatment of tumor cells and antimicrobial therapy. Furthermore, future perspectives are discussed for further developing novel viral nanocarriers or improving existing viral vectors.

Cyclodextrins (CDs) are a series of cyclic oligosaccharides formed by amylose under the action of CD glucosyltransferase that is produced by Bacillus. After being modified by polymerization, substitution and grafting, high molecular weight cyclodextrin polymers (pCDs) containing multiple CD units can be obtained. pCDs retain the internal hydrophobic-external hydrophilic cavity structure characteristic of CDs, while also possessing the stability of polymer. They are a class of functional polymer materials with strong development potential and have been applied in many fields. This review introduces the research progress of pCDs, including the synthesis of pCDs and their applications in analytical separation science, materials science, and biomedicine.

In recent years, researchers across various fields have shown a keen interest in the exploitation of biocompatible natural polymer materials, especially the development and application of seaweed polysaccharides. Seaweed polysaccharides are a multi-component mixture composed of one or more monosaccharides, which have the functions of being anti-virus, anti-tumor, anti-mutation, anti-radiation and enhancing immunity. These biological activities allow them to be applied in various controllable and sustained anti-inflammatory and anticancer drug delivery systems, such as seaweed polysaccharide-based nanoparticles, microspheres and gels, etc. This review summarizes the advantages of alginic acid, carrageenan and other seaweed polysaccharides, and focuses on their application in gel drug delivery systems (such as nanogels, microgels and hydrogels). In addition, recent literature reports and applications of seaweed polysaccharides are also discussed.

Prussian blue nanoparticles (PBNPs) have attracted increasing research interest in immunosensors, bioimaging, drug delivery, and application as therapeutic agents due to their large internal pore volume, tunable size, easy synthesis and surface modification, good thermal stability, and favorable biocompatibility. This review first outlines the effect of tumor markers using PBNPs-based immunosensors which have a sandwich-type architecture and competitive-type structure. Metal ion doped PBNPs which were used as T1-weight magnetic resonance and photoacoustic imaging agents to improve image quality and surface modified PBNPs which were used as drug carriers to decrease side effects via passive or active targeting to tumor sites are also summarized. Moreover, the PBNPs with high photothermal efficiency and excellent catalase-like activity were promising for photothermal therapy and O2 self-supplied photodynamic therapy of tumors. Hence, PBNPs-based multimodal imaging-guided combinational tumor therapies (such as chemo, photothermal, and photodynamic therapies) were finally reviewed. This review aims to inspire broad interest in the rational design and application of PBNPs for detecting and treating tumors in clinical research.