1. Association of increased CCL5 and CXCL7 chemokine expression with neutrophil activation in severe stable COPD
- Author
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Cesare Usai, Fabio Luigi Massimo Ricciardolo, Ian M. Adcock, Paola Brun, Alberto Papi, Gaetano Caramori, A. Di Stefano, Marco Carbone, S Ennio D'Anna, Laura Bristot, Francesca Magno, A. Capelli, Federica Sabatini, Isabella Gnemmi, Kian Fan Chung, Peter J. Barnes, A. Zanini, Bruno Balbi, Marco Contoli, Di Stefano, Caramori, G, Gnemmi, I, Contoli, M, Bristot, L, Capelli, A, Ricciardolo, FL, Magno, F, D'Anna, SE, Zanini, A, Carbone, M, Sabatini, F, Usai, C, Brun, P, Chung, KF, Barnes, PJ, Papi, A, Adcock, I, and Balbi, B
- Subjects
Male ,Pulmonary and Respiratory Medicine ,Chemokine ,Pathology ,medicine.medical_specialty ,COPD, neutrophils, bronchial mucosa, CCL5, CXCL7 ,Bronchi ,Respiratory Mucosa ,Granulocyte ,Neutrophil Activation ,CCL5 ,Pulmonary Disease, Chronic Obstructive ,neutrophils ,Submucosa ,COPD ,Humans ,Medicine ,CXC chemokine receptors ,Chemokine CCL5 ,Aged ,bronchial mucosa ,biology ,Settore BIO/16 - Anatomia Umana ,CD11 Antigens ,business.industry ,CD44 ,Epithelial Cells ,Middle Aged ,respiratory system ,medicine.disease ,Respiratory Function Tests ,respiratory tract diseases ,CXCL1 ,Hyaluronan Receptors ,medicine.anatomical_structure ,Acute Disease ,Immunology ,CXCL7 ,biology.protein ,Female ,Leukocyte Elastase ,business ,Chemokines, CXC ,COPD, CCL5,CXCL7,NEUTROPHIL - Abstract
BACKGROUND: Increased numbers of activated neutrophils have been reported in the bronchial mucosa of patients with stable chronic obstructive pulmonary disease (COPD), particularly in severe disease. OBJECTIVES: To investigate the expression of neutrophilic chemokines and adhesion molecules in bronchial biopsies from patients with stable COPD of different severity (GOLD stages I-IV) compared with age-matched control subjects, smokers with normal lung function and never smokers. METHODS: The expression of CCL5, CXCL1, 5, 6, 7 and 8, CXCR1, CXCR2, CD11b and CD44 was measured in the bronchial mucosa using immunohistochemistry, confocal immunofluorescence, real-time quantitative polymerase chain reaction (RT-QPCR) and Western blotting (WB). RESULTS: The numbers of CCL5+ epithelial cells and CCL5+ and CXCL7+ immunostained cells were increased in the bronchial submucosa of patients with stable severe COPD compared with control never smokers and smokers with normal lung function. This was also confirmed at the level of mRNA expression. The numbers of CCL5+ cells in the submucosa of patients with COPD were 2-15 times higher than any other chemokines. There was no correlation between the number of these cells and the number of neutrophils in the bronchial submucosa. Compared with control smokers, the percentage of neutrophils co-expressing CD11b and CD44 receptors was significantly increased in the submucosa of patients with COPD. CONCLUSION: The increased expression of CCL5 and CXCL7 in the bronchial mucosa of patients with stable COPD, together with an increased expression of extracellular matrix-binding receptors on neutrophils, may be involved in the pathogenesis of COPD.
- Published
- 2009
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