1. In planta comparative transcriptomics of host-adapted strains of Ralstonia solanacearum
- Author
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Tiffany M. Lowe, Stéphane Cruveiller, Isabelle Robène, Caitilyn Allen, Philippe Prior, Florent Ailloud, Peuplements végétaux et bioagresseurs en milieu tropical (UMR PVBMT), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université de La Réunion (UR), Laboratoire de santé des végétaux (LSV Angers), Laboratoire de la santé des végétaux (LSV), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Department of Plant Pathology, University of Wisconsin-Madison, Commissariat Energie Atom & Energie Alternat, Lab Anal Bioinformat Genom & Metab, Evry, France, Partenaires INRAE, Génomique métabolique (UMR 8030), Genoscope - Centre national de séquençage [Evry] (GENOSCOPE), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), UAR 0865 Paris Réunion, Institut National de la Recherche Agronomique (INRA)-Direction des Ressources Humaines (DRH)-Paris Réunion (PARIS REUNION), European Union (ERDF), Conseil Regional de La Reunion, French Agence Nationale de la Recherche, CIRAD, ANSES [11-237/BSL], NIH National Research Service Award [T32 GM07215], Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2015-67011-22799], Prior, Philippe, Laboratoire de la Santé des Végétaux, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), and CNRS, CNRS, UMR 8030, Genom Metab, Evry, France
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0301 basic medicine ,Identification ,Phylogénie ,[SDV]Life Sciences [q-bio] ,Denitrification pathway ,lcsh:Medicine ,Medical and Health Sciences ,Expression des gènes ,2.2 Factors relating to the physical environment ,Aetiology ,Pathotype ,2. Zero hunger ,Ralstonia solanacearum ,Virulence ,Strain (chemistry) ,General Neuroscience ,food and beverages ,General Medicine ,Genomics ,Biological Sciences ,Transcriptomics ,Host adaptation ,General Agricultural and Biological Sciences ,Infection ,Plante hôte ,Pouvoir pathogène ,030106 microbiology ,Biology ,Séquence d'ARN ,Microbiology ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Métabolisme ,Genetics ,Adaptation ,Gene ,H20 - Maladies des plantes ,Génie génétique ,Comparative genomics ,Transcription génique ,Host (biology) ,transcriptomique ,lcsh:R ,Musa ,biology.organism_classification ,Gène ,U30 - Méthodes de recherche - Abstract
Background.Ralstonia solanacearumis an economically important plant pathogen with an unusually large host range. The Moko (banana) and NPB (not pathogenic to banana) strain groups are closely related but are adapted to distinct hosts. Previous comparative genomics studies uncovered very few differences that could account for the host range difference between these pathotypes. To better understand the basis of this host specificity, we used RNAseq to profile the transcriptomes of anR. solanacearumMoko strain and an NPB strain underin vitroandin plantaconditions.Results.RNAs were sequenced from bacteria grown in rich and minimal media, and from bacteria extracted from mid-stage infected tomato, banana and melon plants. We computed differential expression between each pair of conditions to identify constitutive and host-specific gene expression differences between Moko and NPB. We found that type III secreted effectors were globally up-regulated upon plant cell contact in the NPB strain compared with the Moko strain. Genes encoding siderophore biosynthesis and nitrogen assimilation genes were highly up-regulated in the NPB strain during melon pathogenesis, while denitrification genes were up-regulated in the Moko strain during banana pathogenesis. The relatively lower expression of oxidases and the denitrification pathway during banana pathogenesis suggests thatR. solanacearumexperiences higher oxygen levels in banana pseudostems than in tomato or melon xylem.Conclusions.This study provides the first report of differential gene expression associated with host range variation. Despite minimal genomic divergence, the pathogenesis of Moko and NPB strains is characterized by striking differences in expression of virulence- and metabolism-related genes.
- Published
- 2016
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