Search

Your search keyword '"CNIEL Syndifrais"' showing total 9 results
Start Over Author "CNIEL Syndifrais"
9 results on '"CNIEL Syndifrais"'

1. The first dairy product exclusively fermented by Propionibacterium freudenreichii: A new vector to study probiotic potentialities in vivo: The first dairy product exclusively fermented by[i] Propionibacterium freudenreichii[/i]: A new vector to study probiotic potentialities in vivo

Author

Cousin, Fabien, Louesdon, Séverine, Maillard, Marie-Bernadette, Parayre-Breton, Sandrine, Falentin, Hélène, Deutsch, Stéphanie-Marie, Boudry, Gaëlle, Jan, Gwénaël, Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), centre nationale des industries laitières et du lait (CNIEL/SYNDIFRAIS), Alimentation Adaptations Digestives, Nerveuse et Comportementales (ADNC), Institut National de la Recherche Agronomique (INRA), CNIEL/SYNDIFRAIS, Conseil Régional de Bretagne : CBB développement, and Alimentation Adaptions Digestives, Nerveuses et Comportementales (ADNC)

Subjects
intestin, [SDV]Life Sciences [q-bio], food and beverages, propionibacterium, probiotique, fermentation, lait
Abstract

Dairy propionibacteria display probiotic properties which require high populations of live and metabolically active propionibacteria in the colon. In this context, the probiotic vector determines probiotic efficiency. Fermented dairy products protect propionibacteria against digestive stresses and generally contain a complex mixture of lactic and propionic acid bacteria. This does not allow the identification of dairy propionibacteria specific beneficial effects. The aim of this study was to develop a dairy product exclusively fermented by dairy propionibacteria. As they grow poorly in milk, we determined their nutritional requirements concerning carbon and nitrogen by supplementing milk ultrafiltrate (UF) with different concentrations of lactate and casein hydrolysate. Milk or UF supplemented with 50 mM lactate and 5 g L 1 casein hydrolysate allowed growth of all dairy propionibacteria studied. In these new fermented dairy products, dairy propionibacteria remained viable and stress-tolerant in vitro during minimum 15 days at 4 C. The efficiency of milk fermented by the most tolerant Propionibacterium freudenreichii strain was evaluated in piglets. Viability and SCFA content in the colon evidenced survival and metabolic activity of P. freudenreichii. This work results in the design of a new food grade vector, which will allow preclinical and clinical trials.

Published
2012

2. A new dairy product fermented exclusively by dairy propionibacteria: a new tool to study probiotic potentialities in vivo

Author

Cousin, Fabien, Louesdon, Séverine, Maillard, Marie-Bernadette, Madec, Marie-Noelle, Parayre-Breton, Sandrine, Falentin, Hélène, Deutsch, Stéphanie-Marie, Gaillard, Loic, Le Normand, Laurence, Boudry, Gaëlle, Jan, Gwénaël, Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, CNIEL Syndifrais, Systèmes d'élevage, nutrition animale et humaine (SENAH), cniel syndifrais inra, AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), AGROCAMPUS OUEST, and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, intestin, propionibacterium freudenreichii, [SDV.IDA]Life Sciences [q-bio]/Food engineering, food and beverages, fromage, affinage, probiotique, santé, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, produit laitier
Abstract

Propionibacterium freudenreichii is a GRAS bacterium used as a ripening culture. It is also studied for its probiotic potentialities such as the modulation of gut microbiota and enzymatic activities, and of the gut epithelium proliferation/apoptosis balance. These effects require high populations of live and metabolically active P. freudenreichii in the gut. Fermented milks like yoghurts protect P. freudenreichii from digestive stresses but also contain lactic acid starter bacteria. Thus, we developed a new fermented milk with P. freudenreichii as a sole bacterium to specifically investigate its probiotic effects.

Published
2010

3. Challenges underlying the in situ analysis of single bacterial cells by synchrotron FTIR spectromicroscopy

Author

Julie Meneghel, Stéphane Lefrançois, Stéphanie Passot, Frédéric Jamme, Fernanda Fonseca, Paul Dumas, Génie et Microbiologie des Procédés Alimentaires (GMPA), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Synchrotron SOLEIL, CNIEL Syndifrais, and AgroParisTech-Institut National de la Recherche Agronomique (INRA)

Subjects
stomatognathic system, synchrotron FTIR spectromicroscopy, [SDV]Life Sciences [q-bio], bacterial cells, macromolecular substances
Abstract

Challenges underlying the in situ analysis of single bacterial cells by synchrotron FTIR spectromicroscopy. European Conference on the Spectroscopy of Biological Molecules (ECSBM 16)

Published
2015

4. Assessment of the Probiotic Potential of a Dairy Product Fermented by [i]Propionibacterium freudenreichii[/i] in Piglets

Author

Gwénaël Jan, Gaëlle Boudry, Fabien J. Cousin, Sébastien Massart, Yves Le Loir, Sandrine Parayre, Benoît Foligné, Stéphanie-Marie Deutsch, Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Centre National Interprofessionnel de l'Economie Laitière [Paris] (CNIEL), Lactic Acid Bacteria & Mucosal Immunity - CIIL, Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Université Lille Nord de France (COMUE), DNA Vision SA, Alimentation Adaptations Digestives, Nerveuse et Comportementales (ADNC), Institut National de la Recherche Agronomique (INRA), Conseil Régional de Breatgne, CRITT santé (Prodico), CBB Développement ( profusion), CNIEL/Syndifrais, ANR -10-ALIA-016, Centre d’Infection et d’Immunité de Lille (CIIL) - U1019 - UMR 8204 (CIIL), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Alimentation Adaptions Digestives, Nerveuses et Comportementales (ADNC), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, and Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)

Subjects
Male, Colon, [SDV]Life Sciences [q-bio], Sus scrofa, immunomodulation, produit laitier, law.invention, Microbiology, 03 medical and health sciences, Probiotic, Eating, Species Specificity, law, intestin, Animals, Interleukin 8, Food science, Intestinal Mucosa, fermentation, Barrier function, 030304 developmental biology, 2. Zero hunger, chemistry.chemical_classification, 0303 health sciences, biology, 030306 microbiology, Propionibacterium freudenreichii, Tumor Necrosis Factor-alpha, Probiotics, Interleukin-8, Propionibacterium, Fatty acid, food and beverages, General Chemistry, biology.organism_classification, probiotique, In vitro, Milk, chemistry, inflammation, propionibacterium freudenreichii, Fermentation, Female, General Agricultural and Biological Sciences, Ex vivo
Abstract

Dairy propionibacteria, including Propionibacterium freudenreichii , display promising probiotic properties, including immunomodulation. These properties are highly strain-dependent and rarely studied in a fermented dairy product. We screened 10 strains, grown in a newly developed fermented milk ultrafiltrate, for immunomodulatory properties in vitro. The most anti-inflammatory strain, P. freudenreichii BIA129, was further tested on piglets. P. freudenreichii -fermented product improved food intake and growth of piglets. Colonic mucosa explants of treated pigs secreted less interleukin 8 (-25%, P0.05) and tumor necrosis factor α (-20%, P0.05), either in basal conditions or after a lipopolysaccharide challenge. By contrast, the gut structure, barrier function (measured ex vivo in Ussing chambers), microbial diversity (assessed by 16S rRNA pyrosequencing), and colonic short-chain fatty acid content were unchanged, assuming maintenance of normal intestinal physiology. In conclusion, this work confirms in vivo probiotic properties of dairy propionibacteria-fermented products, which are promising for the prevention or healing of inflammatory bowel diseases.

Published
2012

5. SURFING: A national project to understand immunomodulatury effects of dairy starters

Author

Benoît Foligné, Valentin Loux, Christophe Hervé, Jean-René Kerjean, Denis Matter, Maarten van de Guchte, Gwénaël Jan, Lactic Acid Bacteria & Mucosal Immunity - CIIL, Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Unité Mathématique Informatique et Génome (MIG), Institut National de la Recherche Agronomique (INRA), Laboratoires Standa, Institut Technique du Lait et des Produits Laitiers, CNIEL Syndifrais, MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, ANR Surfing (ALIA 2010-016) Valorial, Unité Mathématique, Informatique et Génome (MIG), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Centre d’Infection et d’Immunité de Lille (CIIL) - U1019 - UMR 8204 (CIIL), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Archive Ouverte

Subjects
[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, produit laitier fermenté, intestin, inflammation, propionibacterium freudenreichii, [SDV.IDA]Life Sciences [q-bio]/Food engineering, fromage, [SDV.IDA] Life Sciences [q-bio]/Food engineering, santé, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

absent

Published
2011

6. Promising immunomodulatory effects of selected strains of dairy propionibacteria

Author

Foligné, B., Deutsch, Stéphanie-Marie, Breton, Jérôme, Cousin, Fabien, Parayre-Breton, Sandrine, Dewulf, Joëlle, Thierry, Anne, Falentin, Hélène, Samson, Michel, Lortal, Sylvie, Pot, Bruno, Jan, Gwénaël, Lactic Acid Bacteria & Mucosal Immunity - CIIL, Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, CNIEL Syndifrais, Signalisation et Réponses aux Agents Infectieux et Chimiques (SeRAIC), Université de Rennes (UR), Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), ProdInra, Archive Ouverte, Centre d’Infection et d’Immunité de Lille (CIIL) - U1019 - UMR 8204 (CIIL), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), and Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-IFR140

Subjects
disease prevention, prévention des maladies, fromage, affinage, santé humaine, [SDV.IDA] Life Sciences [q-bio]/Food engineering, human health, probiotique, produit laitier, identification d'espèce, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, inflammation, intestin, propionibacterium freudenreichii, [SDV.IDA]Life Sciences [q-bio]/Food engineering, souche, inflammation intestinale, identification, santé, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, probiotic
Abstract

Inflammatory bowel disease (IBD) constitutes a growing health concern in developed countries. Augmentation of its incidence seems related to the westernized lifestyle, including changes in the diet. The exact mechanisms involved in the etiology of IBD are not completely elucidated, but indigenous intestinal bacteria play a key pro-inflammatory role in it. Selected immunomodulatory probiotic bacteria can counteract inflammation of the intestine through regulatory activities. This involves cross-talk with immune and epithelial cells at the intestine level and induction of regulatory cytokines. Probiotic strains able to induce anti-inflammatory cytokines in human peripheral blood mononucleated cells (PBMCs) in vitro were shown to exert efficient anti-inflammatory effects on colitis in vivo. However, little is known about the immunomodulatory potential of starter bacteria consumed in high amounts in traditional fermented dairy products, including dairy propionibacteria. Available literature, however, suggests an antiinflammatory potential for a strain of P. freudenreichii. We thus investigated immunomodulatory properties of various strains of this bacterium on PBMCs. This included strains used as cheese ripening cultures and strains used as probiotics for human consumption. This investigation revealed a great diversity in the immunomodulatory profile, depending on the strain. One strain, showing a particularly anti-inflammatory profile, was further tested on distinct experimental models of colitis in mice. The promising protective effects observed in vivo suggest that selected strains of dairy propionibacteria and/or fermented dairy products containing these bacteria may be useful in a preventive diet designed to prevent or limit the severity of IBD in humans.

Published
2010

7. Propionibacterium freudenreichii selectively induces apoptosis of cancer, but not healthy, colon epithelial cells

Author

Jan, Gwénaël, Cousin, Fabien, Lan, Annaïg, Jouan, Elodie, Rabot, Sylvie, Brenner, Catherine, Dimanche-Boitrel, Marie-Thérèse, Signalisation et Réponses aux Agents Infectieux et Chimiques (SeRAIC), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Laboratoire de génétique et biologie cellulaire (LGBC), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, CNIEL Syndifrais, AGROCAMPUS OUEST, Laboratoires Standa, Université de Rennes (UR), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Brébion, Alice, Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-IFR140, Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-École pratique des hautes études (EPHE)-Centre National de la Recherche Scientifique (CNRS), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-École pratique des hautes études (EPHE), and ProdInra, Archive Ouverte

Subjects
apoptose, epithelial cell, disease prevention, prévention des maladies, cellule épithéliale, prophylaxie, [SDV.CAN]Life Sciences [q-bio]/Cancer, [SDV.IDA] Life Sciences [q-bio]/Food engineering, probiotique, produit laitier, prévention, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, aliment santé pour l'homme, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, [SDV.CAN] Life Sciences [q-bio]/Cancer, dairy product, intestin, cancer colorectal, propionibacterium freudenreichii, [SDV.IDA]Life Sciences [q-bio]/Food engineering, cancer, colorectal neoplasm, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, probiotic
Abstract

Colorectal cancer constitutes a major concern in developed countries and is tightly linked to environmental factors such as diet. Modification of the diet, including heavily roasted red meat and excess of fat, is in particular regarded as responsible for an increased cancer occurrence. Several studies suggest that it may be avoided via modulation of the diet, including the use of probiotics and prebiotics.Short chain fatty acids (SCFA, mainly butyrate, provided through dietary fibres) play a central protective role in this context and were shown to favour apoptosis of colon cancer cells, yet growth and differentiation of healthy ones. SCFA result mainly from bacterial fermentation of carbohydrates in the intestine and would be responsible for the preventive effect of dietary fibres. However, not all studies support a chemopreventive effect of SCFA, because of a lack of agreement between in vivo and in vitro studies.Dairy propionibacteria constitute promising probiotics in this context and release SCFA, mainly propionate and acetate, in their microenvironment. Indeed, SCFA constitute the final products of their peculiar obligatory fermentation. We have shown that this fermentation occurs within the gut, leading to enhanced intraluminal concentrations of SCFA. Propionibacterial SCFA induce in vitro apoptosis of colon cancer cells (HT-29, Caco-2) through a mitochondriotoxic effect of SCFA and may thus prove useful in the prophylaxis of colorectal cancer.METHODSPropionibacterial SCFA were used to treat both malignant human colorectal cancer cells HT-29 and normal healthy human colonocytes HIEC. Viability was monitored and apoptosis quantified by Hoescht staining and caspases activity assay. Human microbiota associated rats were fed with propionibacteria. The rats were either treated with 1,2-dimethylhydrazine (DMH) or not. Apoptosis and proliferation were quantified in the rats colon using TUNEL and PCNA labelling, respectively.RESULTSWe report here a specific pro-apoptotic effect of dairy propionibacteria. Normal human intestinal epithelial crypt cells HIEC, used as a model of non-cancer cells, were much less susceptible to apoptosis induction by propionibacteria than malignant proliferative HT-29 cells, suggesting a differential sensitivity between normal and transformed cells.Accordingly, specific apoptosis induction by propionibacteria was evidenced in rats treated with the carcinogen 1,2-dimethylhydrazine (DMH). In these mutagenised rats, proliferation was furthermore reduced by the presence of propionibacteria in their diet. By contrast, propionibacteria had no effect in healthy control rats, neither on proliferation, nor on apoptosis, at the level of intestinal mucosa.DISCUSSIONAltogether, these data indicate that probiotic products containing dairy propionibacteria should be considered as part of a preventive diet for the prevention of colorectal cancer or as a dietary supplement used in conjunction with chemotherapy

Published
2010

8. Role of the β-glucan surface polysaccharide in Propionibacterium freudenreichii: physiological and immunomodulatory properties

Author

Foligné, B., Parayre-Breton, Sandrine, Cousin, Fabien, Dewulf, J., Jan, Gwénaël, Deutsch, Stéphanie-Marie, Centre d’Infection et d’Immunité de Lille (CIIL) - U1019 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, CNIEL Syndifrais, Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), ProdInra, Archive Ouverte, and Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS)

Subjects
gène, fromage, affinage, [SDV.IDA] Life Sciences [q-bio]/Food engineering, food quality, beta glucane, probiotique, qualité des aliments, aliment santé, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, polysaccharide, propionibacterium freudenreichii, [SDV.IDA]Life Sciences [q-bio]/Food engineering, health food, immunodétection, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, probiotic, séquençage de gènes
Abstract

P. freudenreichii (PF) is a Gram + species, with the GRAS (Generally Recognized As Safe) status in the USA and a Qualified Presumption of Safety (QPS) status in Europe. It is widely consumed by humans because it is an essential ripening culture in Swiss type cheeses (Emmental, Leerdammer®…), and also as a probiotic in food supplement (Propio-Fidus®). Recently, PF was shown to produce a (1→3, 1→2)-β-D-glucan polysaccharide. A single gene (named gtf) is responsible for the biosynthesis of this surface polysaccharide, that has been immunodetected for 30% of the 100 strains of PF tested. Here we investigated the role(s) of this β-D-glucan.

Published
2010

9. The probiotic Propionibacterium freudenreichii as a new adjuvant for TRAIL-based therapy in colorectal cancer.

Author

Cousin FJ, Jouan-Lanhouet S, Théret N, Brenner C, Jouan E, Le Moigne-Muller G, Dimanche-Boitrel MT, and Jan G

Subjects
Animals, Blotting, Western, Cattle, Cell Cycle drug effects, Cell Proliferation drug effects, Colorectal Neoplasms drug therapy, Colorectal Neoplasms metabolism, Cultured Milk Products, Humans, Membrane Potential, Mitochondrial drug effects, RNA, Messenger genetics, Reactive Oxygen Species metabolism, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, TNF-Related Apoptosis-Inducing Ligand genetics, Transcriptome drug effects, Tumor Cells, Cultured, Apoptosis drug effects, Colorectal Neoplasms pathology, Probiotics pharmacology, Propionibacterium freudenreichii physiology, TNF-Related Apoptosis-Inducing Ligand metabolism
Abstract

TNF-Related Apoptosis-Inducing Ligand (TRAIL) is a well-known apoptosis inducer, which activates the extrinsic death pathway. TRAIL is pro-apoptotic on colon cancer cells, while not cytotoxic towards normal healthy cells. However, its clinical use is limited by cell resistance to cell death which occurs in approximately 50% of cancer cells. Short Chain Fatty Acids (SCFA) are also known to specifically induce apoptosis of cancer cells. In accordance, we have shown that food grade dairy propionibacteria induce intrinsic apoptosis of colon cancer cells, via the production and release of SCFA (propionate and acetate) acting on mitochondria. Here, we investigated possible synergistic effect between Propionibacterium freudenreichii and TRAIL. Indeed, we hypothesized that acting on both extrinsic and intrinsic death pathways may exert a synergistic pro-apoptotic effect. Whole transcriptomic analysis demonstrated that propionibacterial supernatant or propionibacterial metabolites (propionate and acetate), in combination with TRAIL, increased pro-apoptotic gene expression (TRAIL-R2/DR5) and decreased anti-apoptotic gene expression (FLIP, XIAP) in HT29 human colon cancer cells. The revealed synergistic pro-apoptotic effect, depending on both death receptors (TRAIL-R1/DR4, TRAIL-R2/DR5) and caspases (caspase-8, -9 and -3) activation, was lethal on cancer cells but not on normal human intestinal epithelial cells (HIEC), and was inhibited by Bcl-2 expression. Finally, milk fermented by P. freudenreichii induced HT29 cells apoptosis and enhanced TRAIL cytotoxic activity, as did P. freudenreichii DMEM culture supernatants or its SCFA metabolites. These results open new perspectives for food grade P. freudenreichii-containing products in order to potentiate TRAIL-based cancer therapy in colorectal cancer.

Published
2016
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results