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This study discovers the distribution of vegetation in the Sougna Mountain and its modern pollen representation and investigates the relationships with environment and land use variables. A total of 31 moss polsters were collected between 138 and 1364 m above sea-level from wooded to open areas (e.g. cork oak forest, pine reforestation, and shrubland). At each sampling site land use and environment variables were collected. The vegetation in the Sougna Mountain is scattered in a mosaic along three vegetation belts which are displayed in distinct pollen assemblages dominated by the pollen equivalents of the vegetation types (Quercus suber forest, matorrals of Erica spp. and Cistus spp. and Pinus pinaster reforestation). The variation between the variables and pollen data was performed using CCA analysis. The anthropogenic variables such as grazing, and cultivation, contribute to discrimination of the degraded and grazed areas. High precipitation and altitude have a rather good correlation to wooded communities and consequently a good relation to the tree cover. Our results show that the pollen types of Poaceae, Cannabis, Plantago, Nicotiana, Asteraceae, and Urtica are related mostly to human practice and distinguish the disrupted communities. The presence of those taxa in the pollen diagram must be interpreted carefully. A high taxonomic resolution is recommended to prevent the overestimation of the abundance of some taxa. Our results contribute to the advancement of palynological reconstructions of past vegetation and land use in Sougna Mountain and generally in the Rif landscape. [ABSTRACT FROM AUTHOR]

Understanding the growth efficiency of individual trees, or growth per unit of resource utilization, can inform silvicultural management strategies to maximize tree and stand growth. Stand structure—the size and spatial distributions of trees within the stand—strongly influences water, light, and nutrient availability, as well as the resource-use efficiency of each tree. Key silvicultural tools for stand management include manipulating tree density, size distribution, and arrangement by controlling natural regeneration, artificial seeding, planting seedlings, and/or subsequent thinning of established trees. We analyzed two sets of plots from even-aged stands of common coniferous species in central Spain, 106 pure Scots pine (Pinus sylvestris) and 92 pure Mediterranean pine (Pinus pinaster), to examine the dynamics of the dominance hypothesis, the relationship between stand structure and growth, and the relationship between structure and growth efficiency. Our main findings revealed a negative impact of size-class uniformity on stand growth in both Scots pine and Mediterranean pine, while the positive effect of tree size on growth efficiency was supported for Mediterranean pine stands but uncertain for Scots pine. At the operational level, our results highlight how thinning intensity is more important than the thinning method in Mediterranean pinewoods and how thinning can benefit the provision of multiple ecosystem services. We also recommend integrating dominance effects on growth into individual tree modelling. [ABSTRACT FROM AUTHOR]

Pinus pinaster is a very important species for the Galician wood industry. A genetic breeding program was started in the 1980s to select plus trees based on growth and straightness. In this study, we estimated genetic parameters, juvenile-mature correlations and genetic gains in basic density (BD) and the dynamic modulus of elasticity (MOEd) in Galician breeding families, as well as their relation to volume, straightness, and tree slenderness. All traits were measured at a tree age of 12 years in three half-sib progeny trials. Wood quality traits were also measured at 24 years in two other trials. All progeny trials followed a randomized complete block design and the data were analyzed using several mixed-model approaches. The individual heritability of MOEd remained stable at both ages (~ 0.39) but decreased with age for BD (from 0.44 at age 12 to 0.24 at age 24). The high juvenile-mature correlations (0.51 for BD, 0.57 for MOEd) observed support the viability of early selection for both traits at 12 years. Both wood quality traits correlated positively with each other and weakly with volume, straightness and tree slenderness. Selecting a minimum number of the best families, based on volume or any wood quality trait, would generate genetic gains for the selecting trait and prevent genetic losses of non-selecting traits. We also identified specific families showing positive genetic gains for all traits. The research indicates that either or both of these wood quality traits could be included as new selection criteria in the Galician breeding program. [ABSTRACT FROM AUTHOR]

Aim: Marginal tree populations, either those located at the edges of the species' range or in suboptimal environments, are often a valuable genetic resource for biological conservation. However, there is a lack of knowledge about the genetic consequences of population marginality, estimated across entire species' ranges. Our study addresses this gap by providing information about several genetic indicators and their variability in marginal and core populations identified using quantitative marginality indices. Location: Southwestern Europe and North Africa. Methods: Using 10,185 SNPs across 82 populations of maritime pine (Pinus pinaster Ait.), a widespread conifer characterised by a fragmented range, we modelled the relationship of seven genetic indicators potentially related to population evolutionary resilience, namely genetic diversity (based on both all SNPs and outlier SNPs), inbreeding, genetic differentiation, recessive genetic load and genomic offset, with population geographical, demo‐historical and ecological marginality (as estimated by nine quantitative indices). Models were constructed for both regional (introducing gene pool as a random factor) and range‐wide spatial scales. Results: We showed a trend towards decreasing overall genetic diversity and increasing differentiation with geographic marginality, supporting the centre‐periphery hypothesis (CPH). However, we found no correlation between population inbreeding and marginality, while geographically marginal populations had a lower recessive genetic load (only models without the gene pool effect). Ecologically marginal populations had a higher genomic offset, suggesting higher maladaptation to future climate, albeit some of these populations also had high genetic diversity for climate outliers. Main Conclusions: Overall genetic diversity (but not outlier‐based estimates) and differentiation patterns support the CPH. Ecologically marginal populations and those at the southern edge could be more vulnerable to climate change due to higher climate maladaptation, as predicted by genomic offsets, and/or lower potentially adaptive genetic diversity. This risk is exacerbated by typically small effective population sizes and increasing human impact in marginal populations. [ABSTRACT FROM AUTHOR]

Mediterranean forests are very degraded, mainly due to the intensification of wildfires in recent decades, which, boosted by human activity, have contributed to the acceleration of erosion processes and soil degradation. Under certain conditions, this also contributes to the formation of gullies. The aim of this study is to identify and characterise gullies considering their morphological and topographical aspects and determine the factors that control vegetation regrowth in gullies in a Mediterranean environment after a wildfire. The gullies were identified based on the 2018 orthophotograph, after the large wildfire of October 2017 that affected the entire study area. To analyse the vegetation regrowth, we used the normalised difference vegetation index (NDVI) derived from seven Landsat 8 OLI/TIRS images (2017–2022). The Spearman's rho correlation coefficient was selected to estimate the correlation between gully characteristics and vegetation regrowth. Before running the model, a multicollinearity test was conducted (VIF ≤ 10 and tolerance ≥ 0.1). Stepwise multiple regression was conducted in order to identify the independent variable that has a strong relationship with vegetation regrowth. A marginal effects plot was drawn up. The 38 gullies identified are in forest areas, mainly composed of pine (Pinus pinaster) trees (17 gullies) or a combination of pine trees and broadleaf (Eucalyptus globulus) trees (eight gullies). In all, invasive species are present in 11 gullies, alone (one gully), together with pine trees (four gullies) or with other species (six). The other gully has broadleaf trees. The vegetation in the gully channel recovered well in the year after the wildfire. In the following years there was growth at a slower rate until it reached similar values of NDVI in 2022, 5 years after the wildfire. Stepwise multiple regression (SMR) produced a solution with three models. The three derived dimensions covered 66.8% of the variance, considering the mean width, altitude and flow accumulation. The results can help to devise more effective management strategies for areas where the recurrence and intensity of wildfires have contributed very effectively to soil loss and degradation from gully erosion, with a view to a more resilient and sustainable territory. [ABSTRACT FROM AUTHOR]

Heavy metal levels have steadily risen over the past century due to the presence of human activity and are hazardous to human health and the environment. Regarding the health of humans and the environment, palladium (Pd) is among the most hazardous and toxic heavy metals. It is listed as a priority contaminant by the Agency for Toxic Substances and Disease Registry (ATSDR). As a result, eliminating pollution and tracking changes in Pd contamination in the air are the top research priorities. This study will be conducted on trees grown in Düzce, the fifth dirtiest city on the European continent, to identify appropriate species that may be utilized for tracking and mitigating Pd pollution in the air. Samples from Cupressus arizonica, Pinus pinaster, Cedrus atlantica, Pseudotsuga menziesii, and Picea orientalis will all be analyzed as part of the study, and the variations in Pd content according to species, organ, and direction will be assessed. The goal is to ascertain which biomonitors are best suited for tracking and mitigating lead contamination in the atmosphere. The study consistently found Pd contents in woods with different directions that developed within the same period. However, when the species were compared, notable differences were detected between the Pd levels formed in different directions in the same period for each species and the wood formed in the same direction in other periods. This variation suggests that Pd transfer in wood is restricted in all tested species. Consequently, every species performs as a reliable biomonitor for tracking Pd contamination. [ABSTRACT FROM AUTHOR]

The group selection cutting system provides a promising technique for transforming monospecific even-aged stands into uneven-aged, mixed stands. However, this system has scarcely been used to regenerate Mediterranean shade-intolerant species. In this study, we analyze the height increment patterns of Pinus pinaster seedlings emerging in naturally regenerated gaps. P. pinaster is a shade-intolerant pine species distributed throughout the Mediterranean basin. An experimental trial was established in a 50-year-old P. pinaster (maritime pine) plantation located in the mountains of central Spain. Gaps of two different sizes (diameter equal to 1.5 and 2.5 times the dominant height of the stand, 26 and 44 m, respectively) were opened during winter 2017–18. A total of 531 subplots with a 1-m radius were installed in spring 2018, distributed within the gaps as well as in control plots adjacent to the gaps. Seedlings were tagged individually and variables including height, along with other ecological variables, were collected twice a year (in spring and autumn), providing six time intervals for the development of our models. We applied seemingly unrelated regression to analyze the height growth in this polycyclic species, allowing us to model spring and autumn height increments at the same time. Our results showed that seedling height growth depended on the silvicultural treatment, meteorological conditions during the spring growth season, inter-specific scrub competition, position within the gap, and seedling characteristics, such as the ontogenetic development or the size of the seedling itself. This work, along with germination and survival analysis, aims to provide a holistic perspective on the suitability of the group selection cutting system to regenerate maritime pine stands, taking into account current and future climate change scenarios. [ABSTRACT FROM AUTHOR]

Key message: Cistus ladanifer L. shows an extraordinary plasticity of germination and growth. Fire promotes the regeneration of C. ladanifer by triggering its germination through thermal shock as well as by enhancing its seedling growth through the addition of ashes. On the contrary, stacking of Pinus pinaster Ait. leaf litter may hinder seedling establishment, at least in the first months after germination. Context: Pinus pinaster Ait. is affected by forest fires in the Mediterranean basin. Its extreme flammability is due to the high spatial continuity together with the massive accumulation of leaf litter. Cistus ladanifer L. is a species which is widely distributed in the west Mediterranean region where it can form vast shrublands and flourish under these pinewoods. Although high temperatures associated with the occurrence of fire trigger germination of C. ladanifer, knowledge on how other factors, such as the presence of litter on the forest floor or the ash left after the fire, influence germination and seedling growth is essential to improve land management plans. Aims: The main objective of this study was to determine the effects of different loads of litter from P. pinaster and ash from their combustion on the germination and growth of seedlings of C. ladanifer. Methods: Two experiments were carried out to assess the effect of heat shock (100 °C for 10 min) and two loads of litter and ash from P. pinaster (control, high and low loads of litter, high and low loads of ash) on the germination and growth after 2 and 4 months of C. ladanifer. Results: Heat shock significantly increased the germination of C. ladanifer. In contrast, the addition of litter and ash had no effect on total germination but affected the growth of the seedlings coming from seeds exposed to heat shock. Litter treatments reduced biomass of 2 months seedlings and ash increased biomass of 4 months seedlings. Conclusion: C. ladanifer is a species favoured by fire in different ways depending on the stage of regeneration. Germination is promoted by heat shock while seedling growth is favoured by ash nutrients. In contrast, pine leaf litter hinders seedling growth, although this effect disappears 4 months after germination. Alternative forest management practices to prescribed fires are recommended if preventing the spread of Cistus is a priority. [ABSTRACT FROM AUTHOR]

The maritime pine (Pinus pinaster Ait.) is a highly valuable Mediterranean conifer. However, recurrent drought events threaten its propagation and conservation. P. pinaster populations exhibit remarkable differences in drought tolerance. To explore these differences, we analyzed stem transcriptional profiles of grafts combining genotypes with contrasting drought responses under well-watered and water-stress regimes. Our analysis underscored that P. pinaster drought tolerance is mainly associated with constitutively expressed genes, which vary based on genotype provenance. However, we identified key genes encoding proteins involved in water stress response, abscisic acid signaling, and growth control including a PHD chromatin regulator, a histone deubiquitinase, the ABI5-binding protein 3, and transcription factors from Myb-related, DOF NAC and LHY families. Additionally, we identified that drought-tolerant rootstock could enhance the drought tolerance of sensitive scions by regulating the accumulation of transcripts involved in carbon mobilization, osmolyte biosynthesis, flavonoid and terpenoid metabolism, and reactive oxygen species scavenging. These included genes encoding galactinol synthase, CBL-interacting serine/threonine protein kinase 5, BEL1-like homeodomain protein, dihydroflavonol 4-reductase, and 1-deoxy-D-xylulose-5-phosphate. Our results revealed several hub genes that could help us to understand the molecular and physiological response to drought of conifers. Based on all the above, grafting with selected drought-tolerant rootstocks is a promising method for propagating elite recalcitrant conifer species, such as P. pinaster. [ABSTRACT FROM AUTHOR]

Heavy metal concentrations, which have increased continuously in the environment this century due to anthropogenic factors, severely threaten human and environmental health. Antimony (Sb) is one of the most toxic and harmful heavy metals in terms of human and environmental health. Therefore, the priority research subjects are monitoring the alteration of Sb pollution in the airborne and reducing pollution. This study was conducted to conclude the proper species to monitor and drop airborne Sb contamination on trees grown in Düzce, the 5th most polluted European city. This study examined samples taken from Pseudotsuga menziesii, Cupressus arizonica, Pinus pinaster, Picea orientalis, and Cedrus atlantica, and the Sb concentration changes based on tree species, route, tissue, and age range in the last 40 years were evaluated. The study hypothesizes that Sb concentration varies depending on (1) tree species, (2) direction, (3) plant tissue, and (4) age range, all confirmed in this study. In conclusion, the maximum concentrations were achieved in the outer bark and east (5.45 µg g−1) and north directions (6.72 µg g−1), with high traffic density. In addition, the mining and industrial places (sources of metal pollution) are not close to the study area. Therefore, it was concluded that traffic pollution was the primary source of Sb pollution in the study area. The study revealed that C. arizonica is the most suitable species for monitoring and reducing the change in Sb pollution because the highest Sb concentration (4.47 µg g−1) in wood (the largest organ) was obtained in C. arizonica. [ABSTRACT FROM AUTHOR]

Zinc (Zn) shortage is a common micronutrient deficiency affecting plants worldwide, while Zn toxicity may occur when this metal is in excess. Ectomycorrhizal (ECM) fungi are known to be able to modulate the transfer of macro- and microelements, among them Zn, to the plant. However, the underlying mechanisms are not well understood. We identified the HcZnT2 gene from the ECM fungus Hebeloma cylindrosporum, encoding a member of the Cation Diffusion Facilitator (CDF) family including Zn transporters, and analyzed its transcriptional regulation, the transport function by yeast complementation experiments, and its subcellular localization using a GFP fusion protein in yeast. HcZnT2 is highly induced during mycorrhization of Pinus pinaster, and upregulated in presence of the host plant root even without any direct contact. However, HcZnT2 is repressed by Zn excess conditions. By functional expression in yeast, our results strongly support the ability of HcZnT2 to transport Zn and, to a lesser extent, manganese. HcZnT2 localization was associated with the endoplasmic reticulum of yeast. Mycorrhizal gene activation at low external Zn suggests that the Zn transporter HcZnT2 might be important for the early establishment of the ECM symbiosis during Zn deficiency, rather than under Zn excess. HcZnT2 arises as an extremely remarkable candidate playing a key role in Zn homeostasis and regulation in ectomycorrhiza. [ABSTRACT FROM AUTHOR]

The pinewood nematode (PWN), Bursaphelenchus xylophilus, is the causal agent of pine wilt disease and is considered an A2 quarantine organism by the European Plant Protection Organisation. In Europe, this nematode has been reported in Pinus pinaster, P. radiata, and P. nigra. In May 2024, severe wilting symptoms were observed in P. sylvestris trees at Serra da Lousã (Coimbra, the central area of continental Portugal). Wood samples were collected from six wilted trees, and the presence of PWN was investigated. From these, B. xylophilus specimens were detected in five out of the six trees. Species identification was performed based on species-specific morphological diagnostic characters, and this was confirmed by real-time PCR using species-specific primers targeting the B. xylophilus satellite DNA region. This study presents the first detection of B. xylophilus in P. sylvestris in Portugal and in Europe. [ABSTRACT FROM AUTHOR]

We used cluster analysis on 200-year-old tree-ring chronologies to examine the patterns that emerge from self-organization, driven by environmental heterogeneity, that might drive diversification in ponderosa pine (Pinus ponderosa). We determined the natural patterns on the landscape and then tested these groups against historically separated varieties within this species that could be evidence of diversification. We used 178 previously collected tree-ring chronologies from the western United States that were archived in the International Tree-Ring Databank. We explored a variety of clustering techniques, settling on Ward's clustering with Euclidian distance measures as the most reasonable clustering process. These techniques identified two (p = 0.005) to ten (p = 0.01) potential natural clusters in the ponderosa pine chronologies. No matter the number of clusters, we found that the ponderosa pine varieties ponderosa and benthamiana always cluster together. The variety scopulorum differentiates clearly on its own, but brachyptera is a mix of diverse groups, based on the environmental driving factors that control tree-ring chronology variability. Cluster analysis is a useful tool to examine natural grouping on the landscape using long-term tree-ring chronologies, enabling the researcher to examine the patterns of environmental heterogeneity that should lead to speciation. From this analysis, we suggest that the brachyptera variety should be more varied genetically. [ABSTRACT FROM AUTHOR]

The Canadian Fire Weather Index System (CFWIS), empirically developed for forests in Canada, estimates the fuel moisture content ( m f ) at different depths and loads through meteorological parameters. While it is often suggested that adapting an existing fire danger rating system like CFWIS for a new environment requires developing new relationships or modifying existing ones, it is worth considering if such adaptations are always necessary. Based on a dataset of field measurements for surface litter (Pinus pinaster) carried out in the central region of Portugal (2014–2023), we propose a correction of m f based on the Fine Fuel Moisture Code (FFMC) of the CFWIS. This moisture correction was used to determine the Initial Spread Index (ISI) directly and, subsequently, the Fire Weather Index (FWI). Fire records from the study region were used to analyze the performance of the corrected indices. We found that the moisture correction led to higher values and potentially more accurate indices under dry conditions but did not provide a significant improvement in predicting the number of fires and burned areas compared to the original indices. The results suggest that, in relation to fire activity, the CFWIS is sufficiently robust to variations in the fuel moisture content in the study region. [ABSTRACT FROM AUTHOR]

The impregnability of Portuguese maritime pine (Pinus pinaster Ait.) subjected to microwave (MW) drying was tested, and the hydrophobicity, anti-swelling efficiency (ASE), and water repellence efficiency (WRE) were evaluated. Small wood heartwood samples of Portuguese maritime pine and two distinct MW treatment settings were employed. The levels of ASE and WRE of the wood elements were evaluated throughout four cycles of drying in an oven and soaking in water. Because of MW applied energy, the wood pine samples were satisfactorily impregnated with the preservative product. Regarding the absolutely dry densities of the samples, very subtle reductions were measured, and they were statistically equivalent to the average density of the non-MW-treated group. Slight improvements were identified in the WRE values of wood samples dried in the microwave. In terms of ASE, both MW-treated groups had a statistically significant increase. The MW treatment decreased the volumetric swelling of the maritime pine wood specimens. Hence, this study raises new insights and previously unexplored paths that can contribute to the expansion and greater application of MW technology in maritime pine and other species. [ABSTRACT FROM AUTHOR]

Tree species can have great impacts on soil. Since the rhizosphere is more responsive to external inputs than the surrounding bulk soil, we investigated the rhizosphere effect of the exotic slash pine and the native araucaria in long‐term conifer monocultures in southern Brazil. Araucaria trees in a natural section of a nearby mixed araucaria forest were taken as the control. We assessed physical, chemical, and mineralogical properties of the underlying subtropical highly weathered soil. We did not find rhizosphere effects for most chemical and physical soil attributes, yet principal component analysis clearly distinguished the effects of the exotic and native conifers on rhizosphere properties by separating slash pine cluster from reforested and native araucaria clusters. Reductions in silt and sand contents under slash pine reforestation led to increases in clay and well‐crystallized iron oxides contents. The clay mineralogy comprised kaolinite, mica/illite, and hydroxy‐interlayered minerals, with kaolinite enrichment in the rhizospheres of both araucaria sites, mica/illite depletion in the rhizospheres of both araucaria and pine monocultures, and prevalence of well‐crystallized iron oxyhydroxides in the slash pine rhizosphere. This study demonstrates a tendency toward increasing soil weathering in the conifer monocultures, potential negative impacts of the faster growing slash pine reforestation on soil organic carbon, available P, and total K possibly by mica/illite depletion, while araucaria had lower impacts on soil properties. These results show long‐term potential loss of soil fertility and quality, which should be considered when monitoring soil changes in human modified ecosystems. Core Ideas: Monoculture of conifers slightly increased soil particle fragmentation.Conifer monocultures that had not received K fertilizers promoted mica/illite depletion in the rhizospheres.Monoculture of slash pine caused more negative impact on soil in the long term than did monoculture of araucaria. [ABSTRACT FROM AUTHOR]

Climate change-induced hazards, such as drought, threaten forest resilience, particularly in vulnerable regions such as the Mediterranean Basin. Maritime pine (Pinus pinaster Aiton), a model species in Western Europe, plays a crucial role in the Mediterranean forest due to its genetic diversity and ecological plasticity. This study characterizes transcriptional profiles of scion and rootstock stems of four P. pinaster graft combinations grown under well-watered conditions. Our grafting scheme combined drought-sensitive and drought-tolerant genotypes for scions (GAL1056: drought-sensitive scion; and Oria6: drought-tolerant scion) and rootstocks (R1S: drought-sensitive rootstock; and R18T: drought-tolerant rootstock). Transcriptomic analysis revealed expression patterns shaped by genotype provenance and graft combination. The accumulation of differentially expressed genes (DEGs) encoding proteins, involved in defense mechanisms and pathogen recognition, was higher in drought-sensitive scion stems and also increased when grafted onto drought-sensitive rootstocks. DEGs involved in drought tolerance mechanisms were identified in drought-tolerant genotypes as well as in drought-sensitive scions grafted onto drought-tolerant rootstocks, suggesting their establishment prior to drought. These mechanisms were associated with ABA metabolism and signaling. They were also involved in the activation of the ROS-scavenging pathways, which included the regulation of flavonoid and terpenoid metabolisms. Our results reveal DEGs potentially associated with the conifer response to drought and point out differences in drought tolerance strategies. These findings suggest genetic trade-offs between pine growth and defense, which could be relevant in selecting more drought-tolerant Pinus pinaster trees. [ABSTRACT FROM AUTHOR]

Nucleotide-binding domain and leucine-rich repeat (NLR) immune receptor genes form a major line of defense in plants, acting in both pathogen recognition and resistance machinery activation. NLRs are reported to form large gene clusters in limber pine (Pinus flexilis), but it is unknown how widespread this genomic architecture may be among the extant species of conifers (Pinophyta). We used comparative genomic analyses to assess patterns in the abundance, diversity, and genomic distribution of NLR genes. Chromosome-level whole genome assemblies and high-density linkage maps in the Pinaceae, Cupressaceae, Taxaceae, and other gymnosperms were scanned for NLR genes using existing and customized pipelines. The discovered genes were mapped across chromosomes and linkage groups and analyzed phylogenetically for evolutionary history. Conifer genomes are characterized by dense clusters of NLR genes, highly localized on one chromosome. These clusters are rich in TNL-encoding genes, which seem to have formed through multiple tandem duplication events. In contrast to angiosperms and nonconiferous gymnosperms, genomic clustering of NLR genes is ubiquitous in conifers. NLR-dense genomic regions are likely to influence a large part of the plant's resistance, informing our understanding of adaptation to biotic stress and the development of genetic resources through breeding. [ABSTRACT FROM AUTHOR]

Through the shikimate pathway, a massive metabolic flux connects the central carbon metabolism with the synthesis of chorismate, the common precursor of the aromatic amino acids phenylalanine, tyrosine, and tryptophan, as well as other compounds, including salicylate or folate. The alternative metabolic channeling of chorismate involves a key branch-point, finely regulated by aromatic amino acid levels. Chorismate mutase catalyzes the conversion of chorismate to prephenate, a precursor of phenylalanine and tyrosine and thus a vast repertoire of fundamental derived compounds, such as flavonoids or lignin. The regulation of this enzyme has been addressed in several plant species, but no study has included conifers or other gymnosperms, despite the importance of the phenolic metabolism for these plants in processes such as lignification and wood formation. Here, we show that maritime pine (Pinus pinaster Aiton) has two genes that encode for chorismate mutase, PpCM1 and PpCM2. Our investigations reveal that these genes encode plastidial isoenzymes displaying activities enhanced by tryptophan and repressed by phenylalanine and tyrosine. Using phylogenetic studies, we have provided new insights into the possible evolutionary origin of the cytosolic chorismate mutases in angiosperms involved in the synthesis of phenylalanine outside the plastid. Studies based on different platforms of gene expression and co-expression analysis have allowed us to propose that PpCM2 plays a central role in the phenylalanine synthesis pathway associated with lignification. [ABSTRACT FROM AUTHOR]

Pines are the most economically important trees in the world and, together with eucalyptus, they dominate commercial forests. But the success of a relatively small number of widely planted species, such as Pinus pinaster, the maritime pine, comes at a price. Pines are attractive to damaging pathogens and insect pests, including the pinewood nematode (PWN), Bursaphelenchus xylophilus, the causal agent of pine wilt disease (PWD). Originally described in Japan, PWD has caused widespread destruction to forests in countries such as China, Taiwan, Portugal, Spain and the United States. PWN causes irreparable damage to the vascular system of its pine hosts, leading to mortality within 3 months. Pine sawyer beetles (Monochamus spp.) are key vectors of PWD, introducing the PWN to healthy trees during feeding. Other organisms contribute to PWD spread and development, including bacteria, fungi and bark beetles. Control measures include tree felling to prevent vector transmission of PWN, insecticide treatments, trapping of Monochamus spp. and tree breeding for plant resistance. The PWN is a quarantine pathogen and subject to regular legislation and phytosanitary measures aimed at restricting movement and preventing introduction to new areas. Current research is investigating the use of biopesticides against PWN and Monochamus spp. This review examines the biology, epidemiology, impact and management of PWD through published research, grey literature and interviews with people directly involved in the management of the disease in Portugal. [ABSTRACT FROM AUTHOR]

We investigated whether there are significant differences in the climatic response of resin-tapped and untapped maritime pine (Pinus pinaster Ait.) trees in four forest stands in the central mountains of Spain, where the species is of natural origin. The stands are located in different provenance regions, with the westernmost site located on the northern slopes of Sierra de Gredos and the easternmost in the transition to the middle Duero basin, with altitudes ranging from 900 to 1350 m a.s.l. Dominant trees were sampled and standard dendrochronological methods were used to compare tree growth of both types of trees at each site. Climate-tree growth relationships were assessed using bootstrap correlations and response functions between tree-ring growth indices and climate variables, for both partials and full local chronologies of each stand, selecting climatic data from the most complete and closest meteorological stations. Our results showed that the trees selected for resin extraction were older, on average, and when site characteristics were favorable enough for maritime pine growth and resin extraction, all trees were resintapped. Contrastingly, when site characteristics were unfavorable for resin extraction, only the largest and the best-developed trees were tapped. No major differences were found between the climatic responses of resin-tapped and untapped trees, and it was therefore possible to use all the available tree-ring width series per site, both resin-tapped and untapped trees, to identify the main climatic drivers of maritime pine growth in central Spain. In addition, we have expanded the chronologies network of Pinus pinaster in Spain in number and length. This work may provide valuable information to improve forest management strategies for sustainable resin production. [ABSTRACT FROM AUTHOR]

Microwave (MW) drying of wood has gained popularity in the field of wood modification. The rise in temperature during MW drying leads to increased steam pressure, enhancing wood permeability but potentially decreasing mechanical properties. Understanding temperature and moisture behaviors during MW drying is crucial for its industrial application in wood drying. Therefore, this study aimed to characterize the temperature and moisture behaviors during MW drying of small Portuguese maritime pine (Pinus pinaster Aiton.) wood samples to support a wider use of this technology. The effects on water uptake and the compressive strength parallel to the grain were also investigated. The results indicated three distinct phases in the MW drying rates, with an average of 0.085% of water removed per second. Moreover, the temperature underwent three distinct stages: an initial rapid increase, a period of constant temperature, and a slight decrease until drying was complete. At the beginning of MW drying, the temperatures were below 100 °C, with average temperatures ranging from 126 to 145 °C. Specimens with lower initial moisture content had higher temperatures, and a positive correlation was found between initial moisture content and drying time. In contrast, negative correlations were found between the initial moisture content and average temperature, as well as average temperature and MW drying time. Additionally, the operating condition parameters used in MW drying of pine samples enhanced water impregnability by 65%, generating a slight reduction of 11% in compressive strength. It was also noticed that the initial moisture content did not impact MW-dried samples' water uptake or compressive strength. Finally, although small clear wood samples of maritime pine were utilized, the temperature and moisture patterns observed closely matched real-scale specimens. Thus, the findings corroborate a wide utilization of MW technology for wood drying, mainly demonstrating positive possibilities for structural-sized wood specimens. [ABSTRACT FROM AUTHOR]

The modification of fire regimes and their impact on vegetation recovery, soil properties, and fuel structure are current key research areas that attempt to identify the thresholds of vegetation's susceptibility to wildfires. This study aimed to evaluate the vulnerability of Mediterranean pine forests (Pinus halepensis Mill. and Pinus pinaster Aiton) to wildfires, analyzing two major forest fires that occurred in Yeste (Spain) in 1994 and 2017, affecting over 14,000 and 3200 hectares, respectively. Four recovery regions were identified based on fire severity—calculated using the delta Normalized Burn Ratio (dNBR) index—and recurrence: areas with high severity in 2017 but not in 1994 (UB94-HS17), areas with high severity in 1994 but not in 2017 (HS94-UB17), areas with high severity in both fires (HS94-HS17), and areas unaffected by either fire (UB94-UB17). The analysis focused on examining the recovery patterns of three spectral indices—the Normalized Difference Vegetation Index (NDVI), Normalized Moisture Index (NDMI), and Normalized Burn Ratio (NBR)—using the Google Earth Engine platform from 1990 to 2023. Additionally, the Relative Recovery Indicator (RRI), the Ratio of Eighty Percent (R80P), and the Year-on-Year average (YrYr) metrics were computed to assess the spectral recovery rates by region. These three spectral indices showed similar dynamic responses to fire. However, the Mann–Kendall and unit root statistical tests revealed that the NDVI and NDMI exhibited distinct trends, particularly in areas with recurrence (HS94-HS17). The NDVI outperformed the NBR and NDMI in distinguishing variations among regions. These results suggest accelerated vegetation spectral regrowth in the short term. The Vegetation Recovery Capacity After Fire (VRAF) index showed values from low to moderate, while the Vulnerability to Fire (V2FIRE) index exhibited values from medium to high across all recovery regions. These findings enhance our understanding of how vegetation recovers from fire and how vulnerable it is to fire. [ABSTRACT FROM AUTHOR]

A root disease in plantations of Pinus radiata and Pinus pinaster, where trees died in distinct patches, was present in the Western Cape province of South Africa during the 1970s and 1980s. Phytophthora cinnamomi was initially believed to be the cause, but the disease was later ascribed to the insect-associated fungus Leptographium serpens, a fungal species residing in the Ophiostomatales. Doubt regarding the cause of the disease was raised in a later study due to the fact that most Leptographium spp., particularly those that colonise ray parenchyma tissues, which is the case for L. serpens, are not typically primary disease agents. In this study, cultures of an unidentified sterile fungus collected from the dying trees were revived and identified using DNA sequencing methods, which were not available when the disease was first studied. These cultures were identified as the pyrophillic pathogen Rhizina undulata, well-known to cause patch death of conifers in South Africa and elsewhere in the world. While the patches of dying trees no longer exist and the disease cannot be newly studied, it is most likely that the tree death originally thought to be caused by L. serpens was due primarily to R. undulata. The study provides a vivid example of the value of preserving cultures of fungi for later study and the power of modern techniques to identify fungal pathogens. [ABSTRACT FROM AUTHOR]

Wildfires are a natural part of the dynamics of Mediterranean forest ecosystems. The fire patterns in the Mediterranean basin have been altered mainly due to changes in land use and climate change. In 2017, a wildfire in Yeste (Spain) burned 3200 hectares of two Mediterranean pine forests. We investigated the effects of burn severity and postfire salvage logging practices on vegetation and soil properties in four experimental areas distributed within the wildfire perimeter. These areas included unburned, low, high, and high burn severity with salvage logging, all located under Pinus halepensis Mill and Pinus pinaster Aiton stands. Salvage logging was applied 18 months after the fire. We established 72 circular plots (nine per treatment and pine species). We collected soil samples to analyze physicochemical and biological soil properties, including pH, electrical conductivity (EC), soil organic matter (SOM) content, carbon from microbial biomass (CBM), basal soil respiration (BSR), metabolic quotient (qCO2), and two enzymatic activities: β-glucosidase (GLU) and phosphatase (PHP). To understand how vegetation changed after fire, we implemented three linear transects per plot to calculate α-diversity indices (richness, Shannon, and Simpson), vegetation coverage (COBV), fraction of bare soil (BSOIL), the number of postfire seedlings (NSeed) and their average height (Hm), and we grouped vegetation into different postfire adaptive strategies: facultative seeder (R+S+), obligate resprouter (R+S−), obligate seeder (R−S+), and non-fire-adapted (R−S−). We ran ANOVA and Tukey's HSD post hoc tests to evaluate the differences between burn severity and salvage logging practices on the variables examined for each pine stand. We used PCA and correlation analysis to identify plant-soil interactions. Our results suggest that Pinus halepensis stands were more affected by the wildfire than Pinus pinaster stands due to the distinct characteristics of each species (morphology of the leaves, bark thickness, cone structure, etc.) and the significant differences observed in terms of pH, SOM, CBM, qCO2, GLU, PHP, and Nseed. The proportion of obligate resprouter species was higher in Pinus halepensis stands, and the obligate seeder species were higher in Pinus pinaster stands. The study highlighted the importance of monitoring burn severity and postfire management practices to promote forest recovery and reduce wildfire risk. Limiting the negative impact of postfire salvage logging practices can enhance the resilience of vulnerable ecosystems. [ABSTRACT FROM AUTHOR]

We investigated the influence of fire severity, logging of burnt wood, local ecological factors and their interaction on the natural regeneration, survival and growth of maritime pine (Pinus pinaster Ait.), following a fire that took place in 2005. During the period 2006–2020, a sample of 1900 seedlings were monitored, in which three post-fire treatments were applied: (1) Early logging (before seedling emergence); (2) Delayed logging (after emergence); and (3) No management. Multivariate semi-parametric and non-parametric techniques were used to model seedling survival, estimated density and growth of natural pine regeneration. Seedling survival was 31% with a mean density of more than 2000 seedlings/ha at the end of the study period. Logging before seedling emergence was positively related with pine survival and density. Delayed logging resulted in the lowest seedling density and regeneration. Fire severity had a negative influence on regeneration density. The findings indicate that site conditions and fire severity have a stronger influence on natural regeneration of maritime pine than subsequent post-fire management treatments. In order to ensure the presence of maritime pine in pure or mixed stands, silvicultural work is required to control competition from other species and reduce the risk of new wildfires. [ABSTRACT FROM AUTHOR]

The current energy policy targets reducing energy dependence and minimizing pollutant emissions. Therefore, with the growing interest in using biomass as an alternative energy source, conducting scientific studies on its behavior and optimizing the respective conversion systems has become imperative. The present study focuses on investigating the combustion of biochar pellets in a laboratory-scale traveling grate furnace at three different bed temperatures: 700, 750, and 800 °C. The biochars were obtained via the carbonization process of Pinus pinaster, Acacia dealbata, and Cytisus scoparius pellets. The biochar combustion was studied using a moving-bed carbon particle burning model, supported by kinetic information which was obtained via the combustion of the same biochars in a bubbling fluidized bed. The diffusive parameter which was representative of this traveling grate combustion technology was determined, particularly the bed bypass factor. The combustion tests were carried out with the incomplete combustion of the char pellets. In general, the increase in biochar size led to a decrease in the bypass factor. However, the furnace temperature did not influence this parameter. [ABSTRACT FROM AUTHOR]

Forest dynamics are undergoing profound alteration due to the fact that climate change is increasing the frequency and severity of abiotic and biotic forest disturbances. Because of the unpredictable nature of the drought periods and the variation in their severity, Mediterranean forests are typically more vulnerable. Mediterranean Pinus pinaster forests are showing decay symptoms linked to climate change. There is clear evidence that promoting mixtures can serve as an effective forest adaptation strategy. In this regard, we sought to better understand the responses of P. pinaster radial growth dynamics to various factors, in both mixed and pure forest stands, and provide valuable insights into P. pinaster dynamics when mixed with P. sylvestris. In this study, we examined the variation of intra-annual cumulative radial increment patterns in response to the climate of P. pinaster between pure and mixed stands with P. sylvestris. Using data from band dendrometers collected over five consecutive climatically distinct years (2016–2020), a nonlinear mixed-effect model approach was used to analyze the differences in intra-annual cumulative radial increment patterns for P. pinaster between years in mixed and pure stands. The intra-annual radial increment pattern of P. pinaster showed significant year-to-year variation and varied with tree size, with greater increment in larger trees. Trees in mixed stands had a higher mean radial increment compared to corresponding ones in pure stands. Increased summer maximum temperatures negatively affected tree cumulative annual increment regardless of composition, but with a lower impact on trees in pure stands. Spring precipitation increased the length of the growing season, while higher spring maximum temperatures triggered an earlier inflection point. Our results highlight the high plasticity of P. pinaster in adapting to varying intra- and inter-annual environmental conditions and competition with other species and suggest that promoting mixtures with P. sylvestris may be an interesting management strategy for adaptation to climate change. [ABSTRACT FROM AUTHOR]

This article, titled "Leveraging Transnational Networks of Common Gardens: Range-Wide Sites for Pinus pinaster Adaptive Evaluation," explores the genetic adaptation and phenotypic plasticity of Pinus pinaster, a Mediterranean conifer. The study examines the relationship between phylogeographic structure, climate, and genetic and plastic changes in tree growth and survival. The researchers utilized transnational networks of common gardens in Italy, Morocco, and Spain to gather data from over 100 populations of Pinus pinaster. The article includes photographs of the common gardens and provides insight into the diverse conditions and characteristics of the species. [Extracted from the article]

Wildfires act as a selection force threatening the sustainability and diversity of forest genetic resources. Few studies have investigated the genetic effects of forest wildfires. Species with perennial canopy seed banks in serotinous cones and soil or with long-distance seed and pollen dispersion can preserve genetic diversity and population differentiation under normal fire regimes. To test this hypothesis, we characterised molecularly Pinus pinaster Aiton (maritime pine) seedlings produced from seeds sampled in post-fire, naturally regenerated populations that had been subject to different fire regimes in the North of Portugal using inter-simple sequence repeats (ISSRs). The sampled populations burned once (A), twice (B), or three (D) times or had no prior fire history (C, control). Given the globally low seed germination ability, only 104 plantlets regenerated and were described. These plantlets were grouped according to their origin population. Intra-group ISSR polymorphism ranged from 72.73% (B) to 89.41% (D), revealing genetic differentiation among groups originating from populations that had experienced different fire recurrence. Overall, the unaffected genetic diversity of the regenerated plantlets allowed us to accept the hypothesis. Our findings enhance our understanding of the species ability to withstand fire-induced challenges and their responses to wildfires, guiding conservation endeavours and forest management strategies to bolster ecosystem resilience. [ABSTRACT FROM AUTHOR]

Globally, in remaining wildlands, tree densities and forested cover have increased in grasslands and open forests since European settlement. In the southern Rocky Mountains of Colorado, United States, we determined tree composition and tree cover from historical (years 1875 to 1896) surveys and compared them to current (2002 to 2011) tree composition and current (year 2016) forested land cover for 500,000 ha of the Routt National Forest. Additionally, we examined whether changes in precipitation occurred. Regarding composition, pine (primarily lodgepole pine; Pinus contorta) decreased from 65% to 32% of all trees, with increased subalpine fir (Abies lasiocarpa) from 0.5% to 23% of all trees, and quaking aspen (Populus tremuloides) from 13% to 30% of all trees. According to 80% of 5175 survey points not in forests, the historical landscape was very open, comprised of grasslands, mountain meadows, and other open ecosystems. In contrast, 75% of the current landscape is covered by forests. Change points in the Palmer Modified Drought Index were within historical limits, indicating that forestation was not related to a change in water availability. Based on historical surveys and accounts, we envisioned a historical landscape that was open but embedded with dense lodgepole pine clusters and spruce stands at high elevations, which has now become a predominantly forested landscape of dense forests, similar to global forestation patterns. [ABSTRACT FROM AUTHOR]

The EFSA Panel on Plant Health performed a pest categorisation of Dendrolimus punctatus (Lepidoptera: Lasiocampidae), following a commodity risk assessment of bonsai Pinus parviflora grafted onto P. thunbergii from China, in which D. punctatus was identified as a pest of possible concern to the European Union (EU). D. punctatus, also known as the Masson pine caterpillar, is present in China, Taiwan, Vietnam, India and has recently spread to Japanese islands close to Taiwan. Larval feeding on the needles of Pinus elliottii, P. luchuensis, P. massoniana, P. merkusii and P. tabulaeformis causes important damage. D. punctatus larvae can also feed on P. armandii, P. echinata, P. latteri, P. parviflora, P. sylvestris var. mongolica, P. taeda, P. taiwanensis and P. thunbergii, but full development on these hosts is uncertain. The pest has three to five generations per year; winter is spent as larvae on branch tips, on tree trunks and in the soil. The females lay egg clusters on pine needles. Pupation occurs in cocoons attached to branches or needles. D. punctatus could enter the EU either as eggs, larvae or pupae in the foliage of plants for planting or cut branches, as larvae on wood with bark or as overwintering larvae in branches, crevices in the bark or in the litter of potted plants. However, Annex VI of 2019/2072 prohibits the introduction of D. punctatus hosts (Pinus spp.) from countries and areas where the pest occurs. There are climate zones where the pest occurs in Asia that also occur in the EU, though they are limited, which constitutes an uncertainty regarding establishment. The pest's main hosts are not grown in the EU. However, the fact that it attacks the North American Pinus echinata, P. elliottii and P. taeda in its Asian native area suggests a potential capacity to shift to pine species occurring in the EU territory. D. punctatus satisfies all the criteria that are within the remit of EFSA to assess for it to be regarded as a potential Union quarantine pest. Whether the Pinus commonly found in Europe could act as hosts is unknown but is fundamental, affecting the criteria of establishment and magnitude of impact. [ABSTRACT FROM AUTHOR]

Seedling size-related functional attributes of Mediterranean forest species are critical traits to be considered in nursery practices aimed at improving the survival and performance of seedlings exposed to summer drought in dryland reforestation projects. We looked at how nursery light regimes and nitrogen fertilization affected the survival and performance of Pinus pinaster Ait. under contrasting post-planting watering regimes. Seedlings grown in the nursery for 13 months were given low or high nitrogen doses and exposed to three light regimes (full sun, medium light and low light). After outplanting, half of the seedlings were watered fortnightly during the hottest months of the first summer and the other half were left unwatered. Seedling size-related variables (shoot height, basal diameter, stem volume and sturdiness quotient) were measured at the beginning and at the end of the study, and summer drought survival was checked three times. Our results showed that fertilization and the nursery light regime influenced initial seedling quality and transplant stress. Post-planting first summer watering also increased seedling survival probability. The medium-light and especially the full-sunlight regimes in the nursery decreased the probability of seedling mortality after outplanting. However, a low-light regime in the nursery negatively affected plant traits and increased the probability of post-planting seedling mortality. In comparison with low-N seedlings, high-N fertilization increased post-planting stress (evaluated in the change of sturdiness quotient after one field growth season), but did not affect survival. [ABSTRACT FROM AUTHOR]

Background: We aimed to evaluate the impact of two different phytotherapeutic agents on decision making regarding prostate biopsy for patients with higher-than-normal prostate-specific antigen (PSA) levels. Methods: From June 2022 to May 2023, all patients attending two urological institutions due to higher-than-normal PSA levels were randomized to receive either oral capsules of Curcuma Longa, Boswellia, Pinus pinaster and Urtica dioica (Group A) or Serenoa Repens 320 mg (Group B) for 3 months. At the follow-up visit after 3 months, all patients underwent PSA tests and multiparametric magnetic resonance imaging (mpMRI). Results: In the per-protocol analysis, data from 66 patients in Group A and 76 in Group B were analyzed. Fifty patients in Group A (75.7%) showed a significant reduction in total PSA compared to forty-nine in Group B (64.4%) (p < 0.001). Twenty-eight patients had PI-RADS III or higher in mpMRI: twelve in Group A and fourteen in Group B. Twenty-three patients (34.8%) in Group A and fifty-nine (77.6%) in Group B (p < 0.001) underwent prostate biopsy based on the mpMRI findings and PSA values. Three patients in Group A showed a significant reduction in total PSA values while having positive mpMRI findings (6%) compared with nine in Group B (19.5%) (p < 0.001). On the contrary, 7 patients in Group A did not show significant reduction in total PSA values and had negative mpMRI findings (43%) compared to 22 in Group B (81.4%) (p < 0.001). Conclusions: In conclusion, a three-month course of a combination of Curcuma Longa, Boswellia, Pinus pinaster and Urtica dioica seems to be an interesting tool to avoid unnecessary prostate biopsies among men with higher-than-normal PSA levels. [ABSTRACT FROM AUTHOR]

The effects of selection on an organism's genome are hard to detect on small spatial scales, as gene flow can swamp signatures of local adaptation. Therefore, most genome scans to detect signatures of environmental selection are performed on large spatial scales; however, divergent selection on the local scale (e.g. between contrasting soil conditions) has also been demonstrated, in particular for herbaceous plants.Here, we hypothesised that in topographically complex landscapes, microenvironment variability is strong enough to leave a selective footprint in the genomes of long‐lived organisms. To test this, we investigated paired south‐ versus north‐facing Pinus pinaster stands on the local scale, with trees growing in close vicinity (≤820 m distance between paired south‐ and north‐facing stands), in a Mediterranean mountain area. While trees on north‐facing slopes experience less radiation, trees on south‐facing slopes suffer from especially harsh conditions, particularly during the dry summer season.Two outlier analyses consistently revealed five putatively adaptive loci (out of 4034), in candidate genes two of which encoded non‐synonymous substitutions. Additionally, one locus showed consistent allele frequency differences in all three stand pairs indicating divergent selection despite high gene flow on the local scale. Permutation tests demonstrated that our findings were robust.Functional annotation of these candidate genes revealed biological functions related to abiotic stress response, such as water availability, in other plant species.Synthesis. Our study highlights how divergent selection in heterogeneous microenvironments shapes and maintains the functional genetic variation within populations of long‐lived forest tree species, being the first to focus on adaptive genetic divergence between south‐ and north‐facing slopes within continuous forest stands. This is especially relevant in the current context of climate change, as this variation is at the base of plant population responses to future climate. [ABSTRACT FROM AUTHOR]

The maritime pine (Pinus pinaster Ait.) forest is an essential element of the Portuguese forest landscape due to its social, economic, and environmental importance. The sustainability of these forests in the Mediterranean region faces challenges due to recurrent forest fires and the absence of or delayed management. The species has a high capacity for regeneration, but the perpetuation of pine forests in sustainable conditions depends on adequate management to achieve high biomass production and assure fire resilience. This study aimed to analyse four management scenarios (C1 to C4) for the natural regeneration of maritime pine in six areas with stand ages ranging from 6 to 16 years and densities varying from 15,000 to circa 93,000 trees per ha. The same four scenarios were implemented in each of the six areas. The scenarios considered the evolution of forest growth according to different management prescriptions and were simulated using Modispinaster and PiroPinus models. Scenario C1 considered no intervention, with only the final cut. Scenario C2 considered a thinning schedule to maintain the stand within the 50–60% range of the Stand Density Index (SDI). Scenario C3 followed the area's Forest Management Plan (PGF), which typically includes two or three thinning operations throughout the cycle. Scenario C4 was adapted from the MS1 silvicultural model of the National Institute for Nature Conservation and Forests—ICNF, which involves opening strips at earlier ages (3 and 6 years), with the selection of trees to remain in the wooded area carried out between 4 and 10 years of age and performing thinning whenever the Wilson Spacing Factor (FW) reaches 0.21. The final cutting age was assumed to be 45 years but could be lowered to 35 years in Scenario 3 if defined in the plan. Based on the indicators generated by the simulators, the results showed variations in the total volume of timber produced at the time of harvest depending on the silvicultural guidelines. Scenario C4 was the most effective in generating the highest individual tree volume at the end of the cycle and the total volume of timber collected throughout the cycle. The ability of the forest to resist fire was evaluated before and after the first treatment for density reduction. The treatments performed did not decrease the resistance to fire control. [ABSTRACT FROM AUTHOR]

Chromium (Cr) is one of the most detrimental heavy metals, the concentration of which has increased significantly in nature in the last century. Cr pollution can cause toxic and harmful health effects by causing high accumulation levels in animal and human tissues, seriously affecting ecosystems and natural resources. Due to environmental and human health risks, it is critical to track the change of Cr pollution in the air and reduce it. This study aims to determine the suitable tree species that can be used for observing and reducing Cr pollution. For this purpose, samples (wood, inner bark and outer bark) taken from Pinus pinaster, Cupressus arizonica, Picea orientalis, Cedrus atlantica, and Pseudotsuga menziesii species grown in Düzce, Türkiye, which is among the 5 most polluted cities in Europe according to the World Air Pollution 2021 Report, were examined. The accumulation of Cr concentration was determined by species (40-year-old), organ, and direction, and changes in age range were evaluated. As a result of the study, while the highest concentration levels were determined in the outer bark in general, it was interpreted that the branching structure affected this situation. The study results reveal that, in general, Cr pollution is mainly caused by traffic. It has been determined that C. arizonica and P. menziesii are the suitable tree species that can be used for observing and reducing Cr pollution in the air. [ABSTRACT FROM AUTHOR]

Climate change significantly impacts global forests, leading to tree decline and dieback. To cope with climate change, trees develop several functional traits, such as intra-annual density fluctuations (IADFs) in tree rings. The formation of these traits facilitates trees to optimize resource allocation, allowing them to withstand periods of stress and eventually recover when the conditions become favourable again. This study focuses on a Pinus pinaster Aiton forest in a warm, drought-prone Mediterranean area, comparing two growing seasons with different weather patterns. The innovative continuous monitoring approach used in this study combines high-resolution monitoring of sap flow (SF), analysis of xylogenesis and quantitative wood anatomy. Our results revealed the high plasticity of P. pinaster in water use and wood formation, shedding light on the link between IADFs and tree conductance. Indeed, the capacity to form large cells in autumn (as IADFs) improves the total xylem hydraulic conductivity of this species. For the first time, a continuous SF measurement system captured the dynamics of bimodal SF during the 2022 growing season in conjunction with the bimodal growth pattern observed through xylogenesis monitoring. These results highlight the intricate interplay between environmental conditions, water use, wood formation and tree physiology, providing valuable insights into the acclimation mechanisms employed by P. pinaster to cope with weather fluctuations. [ABSTRACT FROM AUTHOR]

Recent forest decline and amplified mortality have been documented around the world, mainly triggered by the rising water stress associated with more frequent extreme weather events. However, other abiotic and biotic factors may predispose and contribute to these processes. Mediterranean forests are among the biomes considered highly vulnerable to drought-induced decline and mortality. Pinus pinaster ssp. mesogeensis , is a typical western Mediterranean Forest species widely distributed in Spain, where traditional management has focused on a mixed timber-resin production. In the last decades, the species has experienced a severe and generalized process of decline and mortality, especially on inland areas in the Spanish Northern Plateau. The main objectives of this study were to (1) provide an accurate assessment of P. pinaster mortality in resin-tapped forests within the region, (2) identify the main predisposing and inciting abiotic factors controlling the process of dieback and mortality of the species and (3) develop a model for forecasting the annual rate of mortality at landscape scale. We used operational data collected by the Forest Service during 2012–2019, including annual censuses of tree mortality carried out in lots under resin-tapping and periodical forest management inventories. Analysis using spatiotemporal generalized linear mixed models indicated widespread mortality of the species in the territory, which in some areas reached 20 per cent of the trees over an 8-year period. Tree mortality is triggered in warm and dry years and was accelerated following the extreme droughts of 2017 and 2019. High stand stocking and tree aging have been identified as predisposing factors increasing susceptibility to forest decline and mortality. In addition, stands where the species grows mixed with Pinus pinea are more vulnerable, pointing to a possible displacement of P. pinaster in the territory. The developed spatiotemporal generalized linear mixed model allows unbiased estimates of the annual rate of mortality to be calculated through the territory. The model may be used by forest managers in order to identify the most vulnerable areas where the application of adaption strategies should be prioritized, in order to preserve these forests and their associated provision of ecosystem services. [ABSTRACT FROM AUTHOR]

Selecting the best resin tapping method and stimulant paste in the resin tapping process is crucial. In timber-oriented rainy Atlantic pine forests of north-west Spain, the interest in resin tapping is raising but information on the best tapping methods and pastes is still lacking. In this study, an appropriate experimental design used on five representative plots of Pinus pinaster, allowed us to explore the resin productive differences between two tapping methods (traditional Spanish method and circular groove) and three stimulant treatments (control, Ethephon and ASACIF). The use of a standardized measure of resin yield allowed to adequately compare methods differing in groove length. Results indicated that the standard resin yield was 1.43 times greater with the traditional method than with the circular groove method. The two stimulant pastes drastically increased resin yield (up to sixfold) in all sites and for all tapping methods. The effectiveness of the paste was also influenced by the tapping method, obtaining greater increases in resin yield after the application of stimulant paste in trees with the circular groove method. Resin yield was only slightly related to the dasometric variables and varied among test sites when no pastes were used, but differences among sites disappeared when stimulant pastes were used. Our results contribute to the understanding of the factors involved in resin performance and the technological development of the sector. [ABSTRACT FROM AUTHOR]

Maritime pine, scientifically known as Pinus pinaster, holds a vital role in Iberian Peninsula forests, primarily as a source of wood for panels, paper, and cellulose production. Recently, there has been a growing interest in utilising agroforestry by-products to yield valuable chemicals for applications in various sectors, including the food, pharmaceutical, and bioenergy industries. This study aimed to assess the value of the primary by-product of Pinus pinaster from the Minho region of northwestern Portugal, i.e., the bark. The research extensively examined the bark's chemical and thermal characteristics, including ash content, extractives, lignin, cellulose, hemicellulose, fatty acids, and mineral composition. Additionally, various analytical techniques like FTIR, SEM, DSC, DTG, and XRD were used to observe chemical structure differences. The results reveal that the Pinus pinaster bark primarily consists of lignin (51.15%) and holocellulose (46.09%), with extractives mainly soluble in toluene–ethanol, followed by water, and a small amount of them are soluble in ethanol. The bark contained around 0.44% ash, and heavy metals such as Cd and Pb were not found. During degradation, Pinus pinaster experienced a 10% mass loss at 140 °C. In terms of crystallinity, holocellulose and cellulose showed similar percentages at approximately 25.5%, while α-cellulose displayed the highest crystallinity index at 41%. [ABSTRACT FROM AUTHOR]

The Natura 2000 Network, as the European Union's ecological network, aims to ensure the long‐term conservation of Europe's most threatened species and habitats, helping to stop the loss of biodiversity. Based on this assumption, the changes in land cover in the Special Area of Conservation (SAC) of Monchique between 1995 and 2018 are analysed and related to identify favourable and unfavourable policies concerning the habitats and respective species conservation. The most important land covers are compared with the habitats and species legally protected at the National and European level, as well as the Red List of Vascular Flora of Mainland Portugal. As main results can be highlighted the increase in the forest area occupied by species such as Eucalyptus globulus and Pinus pinaster, and a reduction in the areas of grazing and shrub species. Can also be noted an increase in the areas covered by invasive species, which may be associated with the abandonment of rural areas. We identified 21 taxa with high heritage interest in the Monchique SAC, occurring mainly in forest habitats, namely 91E0, 92A0, 9240, 9330 and 9340, which should be considered in the elaboration of future Territorial Management Plans. Thus, it can be concluded that the objectives initially stipulated by the Natura 2000 Network were not fully achieved within the Monchique SAC. Alternative management methods must be studied to allow conservation in a territory with a high land consolidation and low population density. [ABSTRACT FROM AUTHOR]

Next‐generation biomonitoring proposes to combine machine‐learning algorithms with environmental DNA data to automate the monitoring of the Earth's major ecosystems. In the present study, we searched for molecular biomarkers of tree water status to develop next‐generation biomonitoring of forest ecosystems. Because phyllosphere microbial communities respond to both tree physiology and climate change, we investigated whether environmental DNA data from tree phyllosphere could be used as molecular biomarkers of tree water status in forest ecosystems. Using an amplicon sequencing approach, we analysed phyllosphere microbial communities of four tree species (Quercus ilex, Quercus robur, Pinus pinaster and Betula pendula) in a forest experiment composed of irrigated and non‐irrigated plots. We used these microbial community data to train a machine‐learning algorithm (Random Forest) to classify irrigated and non‐irrigated trees. The Random Forest algorithm detected tree water status from phyllosphere microbial community composition with more than 90% accuracy for oak species, and more than 75% for pine and birch. Phyllosphere fungal communities were more informative than phyllosphere bacterial communities in all tree species. Seven fungal amplicon sequence variants were identified as candidates for the development of molecular biomarkers of water status in oak trees. Altogether, our results show that microbial community data from tree phyllosphere provides information on tree water status in forest ecosystems and could be included in next‐generation biomonitoring programmes that would use in situ, real‐time sequencing of environmental DNA to help monitor the health of European temperate forest ecosystems. [ABSTRACT FROM AUTHOR]

Pinus pinaster forestry occupies >20% of the forest ecosystem area in the continental territory of Portugal with a high impact on the national economy. This species' major derived non-wood product is oleoresin, the raw material for rosin production. Rosin comprises mainly a blend of resin acids and has broad industrial and pharmaceutical applications. Oleoresin production in Portugal has been progressively reduced due to low-cost producers in other countries; currently, it reaches only 2% of the existing P. pinaster trees. To support this value chain, the chemical fingerprint of rosin derived from the national forest requires focused analysis. In the present study, we collected oleoresin within seven geographically distinct pure P. pinaster forests in two consecutive collection years. A high-resolution nuclear magnetic resonance (NMR) method was used to quantify the diversity of resin acids in the corresponding rosin samples. Overall, the acquired data highlighted that the profile of resin acids in P. pinaster rosin produced in Portugal is highly regular, regardless of the forest location, having as the major constituents abietic acid and dehydroabietic acid. The diversity of resin acids is possibly influenced, to a minor extent, by some edaphoclimatic factors. [ABSTRACT FROM AUTHOR]

Air pollution, a pressing global issue, encompasses various harmful elements, with heavy metals being particularly significant pollutants affecting all forms of life. Effective monitoring and regulation of heavy metal concentrations, especially in the atmosphere, is pivotal. Employing trees as biomonitors emerges as a potent tool, particularly in retrospectively assessing long-term heavy metal contamination trends. This study aims to furnish insights into both tin (Sn) pollutants and the most suitable species for monitoring and mitigating such pollution. Within this study's ambit, samples were collected from Pinus pinaster, Cupressus arizonica, Picea orientalis, Cedrus atlantica, and Pseudotsuga menziesii species in Duzce Province. This area, ranked as the fourth-most air-polluted in Europe according to the World Air Pollution Report, was examined to discern changes in Sn concentration across species, organs, orientations, and age groups over the last four decades. The findings revealed varying potentials for Sn accumulation among the species. Specifically, Pinus pinaster and Picea orientalis were identified as suitable species for monitoring Sn pollution, while Cupressus arizonica, Cedrus atlantica, and Pseudotsuga menziesii exhibited potential for reducing Sn pollution. [ABSTRACT FROM AUTHOR]

High temperatures threaten tree survival and regeneration. A few pine species, such as Pinus oocarpa and Pinus canariensis, resprout after complete defoliation, a likely consequence of evolving in volcanic environments. Pinus pinea and Pinus pinaster rely on other mechanisms to survive wildfires. We hypothesized that the needle water potential (Ψ) and needle osmotic potential (Ψs) would decrease more under hot wind in resprouting species, a strategy of needle sacrifice in accordance with the hydraulic segmentation hypothesis. We submitted two-year-old seedlings to a two-phase hot wind treatment, consisting of one hour at 39 °C followed by five minutes at 70 °C. Phase 2 killed all needles. In non-resprouting species, Ψ decreased steeply at the beginning of Phase 1 and remained between −2 MPa and −4 MPa afterward, maintaining the loss of stem hydraulic conductance below the 50% threshold. On average, resprouting species had 15% lower wood densities and kept 51% higher stem water contents than non-resprouting species after Phase 2. The loss of hydraulic conductance did not affect resprouting. The increase in hydraulic conductance toward the base of the stem was lowest in P. canariensis, suggesting a lower degree of conduit tapering in the only species that had not undergone heteroblastic change. We measured the lowest Ψ and highest Ψs in the most xeric P. canariensis and the opposite in the most mesic P. oocarpa, highlighting the roles of xylary and extra-xylary hydraulic resistances in compartmentalizing the needle to preserve the stem. The measurement of both Ψ and Ψs allowed us to characterize the strategies of response to hot wind in resprouting and non-resprouting pine species. [ABSTRACT FROM AUTHOR]

Summary: Phenotypic plasticity is a main mechanism for sessile organisms to cope with changing environments. Plasticity is genetically based and can evolve under natural selection so that populations within a species show distinct phenotypic responses to environment. An important question that remains elusive is whether the intraspecific variation in plasticity at different spatial scales is independent from each other.To test whether variation in plasticity to macro‐ and micro‐environmental variation is related among each other, we used growth data of 25 Pinus pinaster populations established in seven field common gardens in NW Spain. Phenotypic plasticity to macro‐environmental variation was estimated across test sites while plasticity to micro‐environmental variation was estimated by using semivariography and kriging for modeling within‐site heterogeneity.We provide empirical evidence of among‐population variation in the magnitude of plastic responses to both micro‐ and macro‐environmental variation. Importantly, we found that such responses were positively correlated across spatial scales.Selection for plasticity at one scale of environmental variation may impact the expression of plasticity at other scales, having important consequences on the ability of populations to buffer climate change. These results improve our understanding of the ecological drivers underlying the expression of phenotypic plasticity. [ABSTRACT FROM AUTHOR]

The present study aims to identify the most suitable tree species for monitoring and reducing strontium (Sr) pollution. Strontium is a heavy metal that is extremely harmful to human and environmental health even at low concentrations and is listed as a priority pollutant by the Agency for Toxic Substances and Disease Registry due to its potential harm. Samples were taken from Pinus pinaster, Cupressus arizonica, Picea orientalis, Cedrus atlantica, and Pseudotsuga menziesii species grown in Düzce, a location reported as one of the top 5 cities having the most polluted air in Europe by the World Air Pollution Report. The changes in Sr concentration over the last 40 years were evaluated by species, organ, direction, and age range. The results indicate that Sr pollution significantly increased due to traffic sources. This study also showed that the transfer of Sr within the wood is limited in all the species under consideration; hence, all these species can be used in monitoring the changes in Sr pollution. The most suitable species for reducing Sr pollution were Cupressus arizonica and Picea orientalis, which have the highest capacity to accumulate the most Sr in their wood. [ABSTRACT FROM AUTHOR]

Stand density management diagrams (SDMDs) are robust decision-support tools available to forest managers under limited information. SDMDs which are based on empirical models at stand level, graphically represent the temporal relationships among stand density, and different stand variables such as quadratic mean diameter, dominant height, and mean tree volume. They are used to define initial planting spacing or thinning interventions, to meet various management objectives. Nowadays, there is a growing interest in mixed-species forests as an option for adaptive forest management, where they are considered a guarantor to safeguarding a wide variety of ecosystem services within the framework of sustainability. But there is still a lack of knowledge and efficient tools and models for mixed stands such as SDMDs. This study aims to develop an SDMD for Pinus sylvestris L. and Pinus pinaster Ait. mixed stands in the Sierra de la Demanda (Spain) using data from the third Spanish National Forest Inventory. Both species are two of the most important conifers in Europe and the western Mediterranean basin. Different variables can be used to develop an SDMD. In this case, quadratic mean diameter, dominant height, total stand volume, number of trees per hectare, and stand density index were used. These equations were fit by simultaneous fitting including a new variable representing the proportion of both species in the mixed stand. The results of the simultaneous fitting showed the new variable representing the proportion of both species was not significant. Based on that, the SDMD was constructed without including mixture degree. This SDMD can be used by forest managers as an efficient tool to plan thinning operations. [ABSTRACT FROM AUTHOR]