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Our research aims to explore the possibility of incorporating atorvastatin calcium (ATC) in ultraflexible nanovesicles (UFNVs) to avoid the hepatic first metabolism and enhance its effectiveness as a potential alternative to oral treatment. ATC nanovesicles were developed utilizing the rotary evaporation‐sonication method and assessed in vitro concerning their vesicle diameter, zeta potential (ZP), entrapment efficiency (EE), as well as in vitro release studies. The chosen formulation of ATC‐UFNVs was integrated into a different gel base that was evaluated for content uniformity, pH, spreading ability, viscosity, and rheological behavior. The skin permeation of the prepared nanovesicles was assessed using an ex vivo permeation and confocal laser microscopy images (CLSM). In vitro evaluation of the ATC‐loaded UFNVs showed that the mean diameter of the nanovesicles ranging between 60.84 ± 0.53 nm and 91.68 ± 2.11 nm, the Zeta potential values ranged from of –15.8 ± 0.31 to –25.1 ± 0.81 mV, the EE% were between 84.33 ± 1.94 and 86.53 ± 2.02%, and the ATC release from the formulations was 75.25–90.89%. All release patterns were analyzed kinetically following Higuchi's diffusion approach. Carbapol 934 gel bases containing ATC‐UF exhibited non‐Newtonian pseudo‐plastic flow with thixotropy equal to 2.013 cm2. Ex vivo data showed that ATC‐UFNVs had better penetration compared to free AC. CLSM revealed that the UF‐nanovesicle exhibited stronger and deeper fluorescence signals up to 90 µm depth of permeation in skin versus the rhodamine B‐free solution (50 µm). The transdermal ability of the ATC‐UFNVs gel formulation has been conclusively demonstrated by our research. Practical Applications: The successful development of atorvastatin calcium‐loaded ultraflexible nanovesicles (ATC‐UFNVs) in this study offers promising applications for researchers and healthcare professionals. The ability of these nanovesicles to bypass hepatic first‐pass metabolism and enhance drug delivery to the skin presents a potential alternative to oral administration, which can be associated with gastrointestinal side effects and variable absorption. The findings of this research can be applied in the development of novel transdermal drug delivery systems for the treatment of various cardiovascular conditions. ATC‐UFNVs may be incorporated into topical formulations for the management of hypercholesterolemia, reducing the need for systemic medication. Additionally, the insights gained from this study could be utilized to explore the feasibility of using nanovesicles for the delivery of other lipophilic drugs, expanding their potential therapeutic applications. [ABSTRACT FROM AUTHOR]

Pilostyles, an endoparasitic genus within the Apodanthaceae family, grows inside host stems with flowers and fruits being the only external manifestations. Previous studies of P. berteroi growing on Adesmia trijuga provided limited details of the endophyte and omitted the origin of flowers and sinker structure. This study, using classical methods of optical microscopy applied to the analysis with scanning electron microscopy and confocal laser scanning microscopy, expands the understanding of the P. berteroi/A. trijuga complex. We find that P. berteroi develops isophasically with its host, forming endophytic patches between the host's secondary phloem cells. The parasitized Adesmia stem's cambium primarily produces xylem parenchyma, with limited vessel production and halting fiber formation. The radial polarization of endophytic patches led to the formation of floral meristems. Flowers develop endogenously and emerge by the breakthrough of the host stem. Flowers are connected to the host cambium via chimeric sinkers, combining P. berteroi parenchyma and tracheoids with Adesmia vessels. Unlike previous studies that show uniformity among Pilostyles species, our analysis reveals new insights into the structural interaction between P. berteroi and A. trijuga. [ABSTRACT FROM AUTHOR]

This study assesses river discharges derived using remote sensing and hydrologic modeling approaches throughout the CONUS. The remote sensing methods rely on total water storage anomalies (TWSA) from the GRACE satellite mission and water surface elevations from altimetry satellites (JASON-2/3, Sentinel-3). Surface and subsurface runoff from two Land Surface Models (NOAH, CLSM) are routed using the Hillslope River Routing model to determine discharge. The LSMs are part of NASA's Global Land Data Assimilation System (GLDAS). Differences in key physical processes represented in each model, model forcings, and use of data assimilation provide an intriguing basis for comparison. Evaluation is performed using the Kling Gupta Efficiency and USGS stream gauges. Results highlight the effectiveness of both satellite-derived discharge methods, with altimetry generally performing well over a range of discharges and TWSA capturing mean flows. LSM-derived discharge performance varies based on hydroclimatic conditions and drainage areas, with NOAH generally outperforming CLSM. CLSM-derived discharges may be impacted by the use of data assimilation (GLDAS v2.2). Low correlation and high variability contribute to lower KGE values. GLDAS models tend to perform poorly in snow dominated, semi-arid and water-regulated systems where both the timing and magnitude of the simulated results are early and overestimated. [ABSTRACT FROM AUTHOR]

This study aimed to develop and evaluate a niosomal formulation loaded with Plumbago zeylanica extract (PZE). The solvent evaporation method was used to prepare niosomes and optimized using the Box–Behnken design. The optimized formulation (PZE-Ns-Opt) was characterized for drug release, DPPH assay, α-amylase inhibition assay, α-glucosidase inhibition assay, and confocal laser scanning microscopy (CLSM) study. PZE-Ns-Opt showed a vesicle size of 253.6 nm, PDI of 0.108, entrapment efficiency of 62.4%, and drug release of 84.01%. The CLSM image of the rat's intestine suggested that the rhodamine red B-loaded PZE-Ns-Opts showed superior penetration compared to the control. Further, the antioxidant activity of the prepared formulation was exhibited as 89.46%±0.016 as compared to PZE (78.10%±0.005). In addition to this, α- amylase activity was inhibited by 95.11%±4.62, 85.88%±2.56, and 89.87%±3.65 by acarbose, PZE, and PZE-Ns-Opt, respectively. In comparison, the α-glucosidase activity was inhibited by 88.47%±1.04, 81.07%±0.50 and 85.78%±0.71 by acarbose, PZE, and PZE-Ns-Opt, respectively. To conclude, the development of PZE-Ns-Opt formulation and its characterization showed the establishment's authenticity. The in vitro antidiabetic, antioxidant studies expressed the protective effect from oxidative stress, CLSM of rat's intestine ability to penetrate to great extent and could be a promising candidate for the management of diabetes mellitus. [ABSTRACT FROM AUTHOR]

Aerosol whipped cream, widely used in various foods, relies on solid fats rich in saturated fatty acids (SFAs) for stable gas entrapment. In this study, the potential of oleogels as a healthy fat substitute for formulating aerosol whipped cream was studied. The analysis focused on the effects of different types (beeswax [BW], rice bran wax [RW], and carnauba wax) and the quantities of wax on the properties of the aerosol whipped creams. The BW‐oleogel‐based aerosol whipped cream exhibited the highest foam ability and foam stability. The superior physical properties of the bees wax‐oleogel were attributed to the higher overrun and height stability of its whipped cream compared to RW and carnauba oleogels. The 6% BW‐whipped cream showed significantly higher overrun and cream stability. The 6% BW oleogel whipped cream contained 4.4 times lower SFAs than the dairy milk fat whipped cream. This study represents the first exploration into the feasibility of formulating oleogel‐based aerosol whipped cream using liquid vegetable oil. [ABSTRACT FROM AUTHOR]

Aim: The osteogenic potential of new premixed calcium‐silicate‐containing bioceramic sealers (Ca‐Si sealers) was tested with porcine vascular wall‐mesenchymal stem cells (pVW‐MSCs). Methodology: Two Ca‐Si‐containing sealers: Ceraseal (MetaBiomed, Cheong‐si, South Korea) and AH Plus Bioceramic (Maruchi, Wonju‐si, South Korea), and an epoxy resin sealer (AH Plus; Dentsply, Konstanz, Germany) as a control, were prepared according to the manufacturers' indications. All samples were allowed to set for 100% of their setting time in a sterile humid cabinet at 37°C and 95% relative humidity. pVW‐MSC seeding efficiency and osteogenic differentiation were analysed as marker of gene/protein expression for up to 12 days. Mineralization assay and immunofluorescence staining were performed and evaluated over a period of 21 days. Statistical analyses were conducted using one‐way analysis of variance (p <.05). Additional samples were prepared and stored under the same conditions and inspected using an environmental scanning electron microscope equipped with an energy dispersive X‐ray spectroscopy system. Results: Significantly higher cell seeding efficiency (p <.05) was observed for both Ca‐Si sealers from day 8. pVW‐MSCs showed a significant shift towards the osteogenic lineage only when seeded in contact with Ca‐Si sealers. Gene expression of osteopontin was upregulated significantly. Collagen I and osteocalcin were clearly expressed by cells in contact with Ca‐Si sealers. Mineralization granules were observed in Alizarin red assays and confocal laser scanning microscopy analysis of both Ca‐Si sealers. No gene expression or granule mineralization were observed on the epoxy resin sealer. Conclusions: Premixed Ca‐Si sealers displayed a higher potential for osteogenic activity on pVW‐MSCs. Epoxy resin sealer was unable to induce any osteogenic activity. The properties of both Ca‐Si sealers suggest their potential as osteoinductive platforms for vascular MSCs in periapical bone. [ABSTRACT FROM AUTHOR]

This study aimed to evaluate the efficacy of different irrigation techniques including standard needle irrigation (SNI), passive ultrasonic irrigation (PUI), EndoActivator (EA), XP‐Endo Finisher (XPF), photon‐induced photoacoustic streaming (PIPS), and shock wave enhanced emission photo‐acoustic streaming (SWEEPS) systems on the penetration of irrigation solutions into dentinal tubules in teeth with calcium hydroxide (CH) applied and oval‐shaped canals by confocal laser scanning microscope (CLSM). Ninety mandibular incisor teeth with oval‐shaped canals were included in this study. After the preparation of teeth using Resiproc Blue R25 (VDW, Munich, Germany) canals were filled with a paste based on CH. The teeth were stored in 100% humidity at a temperature of 37°C for 14 days. According to the irrigation activation systems, the teeth were divided into six groups (n = 15); SNI, PUI, EA, XPF, PIPS, and SWEEPS. In each group, 3 irrigation/activation cycles of 20 s of irrigation and 20 s of activation were performed with the relevant activation method. The root canals were filled with fluorescein sodium (Sigma, Alldrich Co., St. Louis, MO, USA) and activated with the relevant activation method for 30 s. Specimens were sectioned horizontally to 1 ± 0.1 mm at 2, 5, and 8 mm from the apex and then examined under the CLSM. Maximum penetration depth, maximum penetration area and penetration percentage were measured by using Image J software. Data were analyzed using a two‐way analysis of variance (ANOVA) and posthoc Tukey tests (p =.05). No difference was found between the activation systems in terms of maximum penetration depth and maximum penetration area of irrigation solutions in the apical section (p >.05). The penetration percentage of irrigation solutions was higher in PUI and PIPS compared with EA and XPF at the apical section (p <.05). No difference was found between SNI, PUI, PIPS, and SWEEPS in terms of the penetration percentage of irrigation solutions in all sections (p >.05). The penetration percentage of irrigation solutions was higher in the middle and coronal sections compared to the apical section in EA and XPF (p <.05). Tubule penetration of irrigation solutions in SNI was similar between sections. The Tubule penetration area of irrigation solutions in PUI, EA, XPF, PIPS, and SWEEPS was lower in the apical section than in the middle and coronal sections. Research Highlights: The tubule penetration depth and area of the irrigation solutions were similar between the activation systems tested in the apical sections.The Tubule penetration area of irrigation solutions in middle and coronal sections was higher in PUI and PIPS than in SNI. [ABSTRACT FROM AUTHOR]

Abstract The aim of the study is to assess the significance of storage duration on fermented Soghurt (soy curd), focusing on changes in fat and protein biomolecules analyzing with CLSM, viscosity measurements, and particle size distributions under refrigerated conditions. Soghurt prepared with 1% yogurt culture (Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus thermophilus) was analyzed for storage stability under refrigerated conditions for up to 15 days measuring the pH, acidity, microbial counts, viscosity, particle size distribution and scanning electron microscopy for structural analysis. Even though lactic counts were not affected significantly during the storage periods, yeast & mold counts and coliform counts were absent up to 15 days during the storage. However, the product could be stored upto 7 days under refrigeration condition (6–8 °C). During the storage period, it was observed that the viscosity of soghurt increased with increase in storage time. Particle size distribution was estimated during the entire storage periods for soghurt and it was observed that smaller particle was mostly found in the range of 3.0–50.0 µm while bigger particles were in the range of 57.0–910.0 µm. Further, larger aggregates with big particle sizes were observed during prolonged storage period. The CLSM study showed the appearance of proteins and fats being dispersed slowly during the storage due to the proteolytic and lipolytic activities of yoghurt cultures after 5 days of storage under refrigeration condition.

Abstract This study assesses river discharges derived using remote sensing and hydrologic modeling approaches throughout the CONUS. The remote sensing methods rely on total water storage anomalies (TWSA) from the GRACE satellite mission and water surface elevations from altimetry satellites (JASON-2/3, Sentinel-3). Surface and subsurface runoff from two Land Surface Models (NOAH, CLSM) are routed using the Hillslope River Routing model to determine discharge. The LSMs are part of NASA’s Global Land Data Assimilation System (GLDAS). Differences in key physical processes represented in each model, model forcings, and use of data assimilation provide an intriguing basis for comparison. Evaluation is performed using the Kling Gupta Efficiency and USGS stream gauges. Results highlight the effectiveness of both satellite-derived discharge methods, with altimetry generally performing well over a range of discharges and TWSA capturing mean flows. LSM-derived discharge performance varies based on hydroclimatic conditions and drainage areas, with NOAH generally outperforming CLSM. CLSM-derived discharges may be impacted by the use of data assimilation (GLDAS v2.2). Low correlation and high variability contribute to lower KGE values. GLDAS models tend to perform poorly in snow dominated, semi-arid and water-regulated systems where both the timing and magnitude of the simulated results are early and overestimated.

Calceoli are putative sensory organs which are known in limnic, marine, and subterranean amphipods for almost 200 years. Despite an otherwise comprehensive understanding of the sensory organs of crustaceans, we still have not unambiguously understood their function. Since calceoli are mainly found on the antennae of male animals, previous studies suggested a chemosensory function mainly related to reproduction. Here, we use a combination of light and electron microscopic techniques to examine the calceoli of Gammarus locusta (Linnaeus 1758) and Oediceroides calmani (Walker 1906), to provide an overview over these structures, and in addition reveal nervous tissue in close proximity to the calceoli. The calceoli of both species are cuticular structures and consist of proximal and distal elements, a stalk and a receptacle that connects both regions. The two studied calceoli differ in the structure of their proximal and distal element, as well as in their receptacle. This study provides new insight into the functional morphology of the antenna and calceolus. Histological sections through the antennae and the calceoli indicate that the calceoli might possess a mechanosensory function. [ABSTRACT FROM AUTHOR]

In recent years, fluorescence microscopy has revitalized the study of invertebrate comparative morphology. Here we explore the usefulness of combining confocal laser scanning microscopy (CLSM) and cuticular autofluorescence to examine the taxonomically challenging marine planktonic "y-larvae" (Pancrustacea: Facetotecta). To gauge the effectiveness of CLSM with autofluorescence in producing taxonomically useful images, we applied it to seven distinct y-naupliar species or morphospecies that had previously undergone scrutiny by other techniques. The specimens were part of a museum collection of glycerin-jelly slides of exuviae of last-stage y-nauplii, a key instar for studying the taxonomy of y-larvae. For Hansenocaris demodex, the level of detail obtained from a single specimen was comparable to that previously obtained by scanning electron microscopy (SEM). For Hansenocaris aquila, revisiting the original holotype specimen resulted in a dramatic increase in our understanding of the species' morphology, including taxonomically pivotal information about its spinose labrum and a digitally rotated lateral view. CLSM analyses of the other five specimens, which represented a broad spectrum of y-naupliar morphology, efficiently generated more such information. Novel observations were made concerning putative external rudiments of both the first and second maxillae in late nauplii as well as the extreme dorso-ventral flattening of some naupliar types. CLSM observation of museum slides of naupliar exuviae using cuticular autofluorescence thus shows great promise of becoming an excellent tool for studying the morphology and taxonomy of y-larvae, and we suggest that this technique might also profitably be applied to other forms of larval exuviae. [ABSTRACT FROM AUTHOR]

Membrane surface fouling has always been a critical issue for the long-term operation of polymeric membranes. Therefore, it is crucial to develop new approaches to prevent fouling. While developing new approaches, characterization methods are greatly important for understanding the distribution of fouling on the membrane surface. In this work, a cellulose acetate membrane was fouled by the filtration of artificial wastewater based on alginate. The surfaces of fouled membranes were characterized through scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), atomic force microscopy (AFM), and white light interferometry (WLI). The results were then compared in terms of the resolution, accuracy, feasibility, and cost-efficiency. [ABSTRACT FROM AUTHOR]

Purpose of Review: Quantification of the morphology of osteocyte lacunae has become a powerful tool to investigate bone metabolism, pathologies and aging. This review will provide a brief overview of 2D and 3D imaging methods for the determination of lacunar shape, orientation, density, and volume. Deviations between 2D-based and 3D-based lacunar volume estimations are often not sufficiently addressed and may give rise to contradictory findings. Thus, the systematic error arising from 2D-based estimations of lacunar volume will be discussed, and an alternative calculation proposed. Further, standardized morphological parameters and best practices for sampling and segmentation are suggested. Recent Findings: We quantified the errors in reported estimation methods of lacunar volume based on 2D cross-sections, which increase with variations in lacunar orientation and histological cutting plane. The estimations of lacunar volume based on common practice in 2D imaging methods resulted in an underestimation of lacunar volume of up to 85% compared to actual lacunar volume in an artificial dataset. For a representative estimation of lacunar size and morphology based on 2D images, at least 400 lacunae should be assessed per sample. [ABSTRACT FROM AUTHOR]

In part I, we reported Hansen solubility parameters (HSP, HSPiP program), experimental solubility at varied temperatures for TOTA delivery. Here, we studied dose volume selection, stability, pH, osmolality, dispersion, clarity, and viscosity of the explored combinations (I-VI). Ex vivo permeation and deposition studies were performed to observe relative diffusion rate from the injected site in rat skin. Confocal laser scanning microscopy (CLSM) study was conducted to support ex vivo findings. Moreover, GastroPlus predicted in vivo parameters in humans and the impact of various critical factors on pharmacokinetic parameters (PK). Immediate release product (IR) contained 60% of PEG400 whereas controlled release formulation (CR) contained PEG400 (60%), water (10%) and d-limonene (30%) to deliver 2 mg of TOTA. GastroPlus predicted the plasma drug concentration of weakly basic TOTA as function of pH (from pH 2.0 to 9). The cumulative drug permeation and drug deposition were found to be in the order as B-VI˃ C-VI˃A-VI across rat skin. This finding was further supported with CLSM. Moreover, IR and CR were predicted to achieve Cmax of 0.0038 µg/ mL and 0.00023 µg/mL, respectively, after sub-Q delivery. Added limonene in CR extended the plasma drug concentration over period of 12 h as predicted in GastroPlus. Parameters sensitivity analysis (PSA) assessment predicted that sub-Q blood flow rate is the only factor affecting PK parameters in IR formulation whereas this was insignificant for CR. Thus, sub-Q delivery CR would be promising alternative with ease of delivery to children and aged patient. [ABSTRACT FROM AUTHOR]

Three-dimensional obturation of the root canal system is the primary goal of endodontic treatment. Endodontic sealers should fill any irregularities in the root canal system and penetrate as deeply as possible into the dentinal tubules. They should also ensure that the gutta-percha points adhere well to the dentinal walls of the root canal without leaving voids at their margins. The purpose of this study was to evaluate contemporary imaging techniques used for in vitro testing of selected specimens consisting of tooth scrapings after endodontic treatment with gutta-percha and selected endodontic sealers: AH Plus-Dentsply Sirona, Well Root ST-Vericon, Korea, and AH Bioceramics Sealer-Dentsply Sirona. The following imaging techniques were used in the evaluation: scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), micro-computed tomography (micro-CT), surgical microscopy, and light microscopy. A comparison of the imaging methods (SEM, CLSM, micro-CT, surgical microscopy, light microscopy) showed significant differences in their suitability for assessing the penetration depth of endodontic sealers into dentinal tubules in vitro. Based on the analysis of the imaging methods, it was concluded that confocal microscopy and SEM were the most objective and accurate methods for visually assessing the quality of root canal filling in vitro. However, the high cost of specimen preparation and the limited availability of specialized equipment are factors that limit their use in clinical settings. [ABSTRACT FROM AUTHOR]

Objective: The objective of this study was to evaluate the effect of blood and artificial salivary contamination of different root-end filling materials on microleakage using a confocal laser scanning microscope and on marginal adaptation using a scanning electron microscope. Materials and Methods: Eighty noncarious single-rooted teeth with mature apices were taken. After retro-cavity preparation, they were randomly assigned into two major groups (n = 40). They were contaminated with blood and artificial saliva, respectively. Each major group was divided randomly into four subgroups (n = 10) and filled as follows: Subgroup A, Biodentine; Subgroup B, bioactive bone cement; Subgroup C, Cention N; and Subgroup D, Bio-C Repair. The samples were sectioned transversely at 1 and 2 mm from the root apex and checked under a confocal laser scanning microscope for microleakage and under an scanning electron microscope for marginal adaptation. The average mean values were calculated. Independent samples t-tests, paired t-tests, and one-way analysis of variance with Tukey's post hoc tests were done to analyze the data. Results: All the tested materials showed marginal gaps and dye leakage. The Bio-C Repair group showed the least mean marginal gap and dye leakage values, followed by bioactive bone cement, Biodentine, and Cention N, respectively, in both blood and artificial saliva contamination. However, the mean marginal gaps and dye leakage between the major groups were statistically insignificant. Conclusion: In an overall comparison, Bio-C Repair was found to be superior in terms of marginal adaptation and sealing ability under the test conditions. [ABSTRACT FROM AUTHOR]

BACKGROUND RESULTS CONCLUSION The present study aimed to evaluate the anti‐hypertensive and anti‐diabetic activities from biologically active peptides produced by fermented sheep milk with Lacticaseibacillus paracasei M11 (MG027695), as well as to purify and characterize the angiotensin‐converting enzyme (ACE) inhibitory and anti‐diabetic peptides produced from fermented sheep milk.After 48 h of fermentation at 37 °C, sheep milk demonstrated significant changes in anti‐diabetic effects and ACE‐I effects, with inhibition percentages observed for ACE inhibition (76.32%), α‐amylase (70.13%), α‐glucosidase (70.11%) and lipase inhibition (68.22%). The highest level of peptides (9.77 mg mL–1) was produced by optimizing the growth conditions, which included an inoculation rate of 2.5% and a 48 h of incubation period. The comparison of molecular weight distributions among protein fractions was conducted through sodium dodecyl‐sulfate polyacrylamide gel electrophoresis analysis, whereas spots were separated using 2D gel electrophoresis according to both the molecular weight and pH. Peptide characterization with ultra‐filtration membranes at 3 and 10 kDa allowed the study to assess molecular weight‐based separation. Nitric oxide generated by lipopolysaccharide and the secretion of pro‐inflammatory cytokines in RAW 264.7 immune cells were both inhibited by sheep milk fermented with M11. Fourier‐transform infrared spectroscopy was employed to assess changes in functional groups after fermentation, providing insights into the structural changes occurring during fermentation.The present study demonstrates that fermentation with L. paracasei (M11) led to significant changes in fermented sheep milk, enhancing its bioactive properties, notably in terms of ACE inhibition and anti‐diabetic activities, and the generation of peptides with bioactive properties has potential health benefits. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Aim: The aim of the study was to evaluate and compare the depth of penetration of adhesive resin in resin-modified glass ionomer cement (RMGIC) after different surface pretreatment methods using a confocal laser scanning microscope (CLSM). Methods: Class I cavity preparation was done in 45 freshly extracted premolars and divided into three groups, according to the surface pretreatment of RMGIC which was placed in a thickness of 1 mm in the cavity. In the control group, no pretreatment was done, and other two groups were pretreated with acid etching and air abrasion (AA). The fifth-generation dentin bonding agent mixed with rhodamine B dye 0.1% was applied on RMGIC in all the samples and cured for 15 s. Samples were restored using composite resin and sectioned longitudinally. The depth of penetration of adhesive resin was evaluated using CLSM. Results: Depth of penetration of adhesive resin was highest with AA (153.70 ± 10.23), followed by acid etching (122.71 ± 12.25) and control group (77.12 ± 6.37). Conclusion: Based on the findings of this research, AA enhances the depth of penetration of adhesive resin in the RMGIC surface. Thus, AA before placement of composite resin on the RMGIC can be effective in a clinical scenario. [ABSTRACT FROM AUTHOR]

Mitochondria are the energy factories of a cell, and depending on the metabolic requirements, the mitochondrial morphology, quantity, and membrane potential in a cell change. These changes are frequently assessed using commercially available probes. In this study, we tested the suitability of three commercially available probes—namely 5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolo-carbocyanine iodide (JC-1), MitoTracker Red CMX Rox (CMXRos), and tetramethylrhodamine methyl ester (TMRM)—for assessing the mitochondrial quantity, morphology, and membrane potential in living human mesoangioblasts in 3D with confocal laser scanning microscope (CLSM) and scanning disk confocal microscope (SDCM). Using CLSM, JC-1, and CMXRos—but not TMRM—uncovered considerable background and variation. Using SDCM, the background signal only remained apparent for the JC-1 monomer. Repetitive imaging of CMXRos and JC-1—but not TMRM—demonstrated a 1.5–2-fold variation in signal intensity between cells using CLSM. The use of SDCM drastically reduced this variation. The slope of the relative signal intensity upon repetitive imaging using CLSM was lowest for TMRM (−0.03) and highest for CMXRos (0.16). Upon repetitive imaging using SDCM, the slope varied from 0 (CMXRos) to a maximum of −0.27 (JC-1 C1). Conclusively, our data show that TMRM staining outperformed JC-1 and CMXRos dyes in a (repetitive) 3D analysis of the entire mitochondrial quantity, morphology, and membrane potential in living cells. [ABSTRACT FROM AUTHOR]

Infectious diseases, including urinary tract infections (UTIs), significantly impact global health, diminishing the quality of life and increasing clinical and economic burdens. Concerns arise due to the potential development of antimicrobial resistance (AMR) facilitated by biofilm formation. Nanotechnology, specifically silver and silver nanoparticles (1-100 nm), proves effective against bacterial strains, making them valuable in the biomedical field. Advances in microscopic imaging techniques, such as Confocal Laser Scanning Microscopy (CLSM), enhance our understanding of biofilms. This study aims to assess the effectiveness and optimal concentration of gold and silver nanoparticles in combating biofilms formed by uropathogenic Escherichia coli (UPEC) through CLSM analysis. The research comprises the following stages: rejuvenation of bacterial isolates and inoculum preparation, testing the anti-biofilm activity of gold and silver nanoparticles at concentrations of 25, 50, 75, and 100 ppm against UPEC using CLSM analysis. The findings reveal that both gold and silver nanoparticles exhibit anti-biofilm activity against UPEC at concentrations of 25, 50, 75, and 100 ppm, as confirmed by CLSM analysis. The optimal concentration for anti-biofilm activity of gold and silver nanoparticles against UPEC is identified as 100 ppm. [ABSTRACT FROM AUTHOR]

Copyright of New Building Materials / Xinxing Jianzhu Cailiao is the property of New Building Materials Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The antimicrobial activity of Origanum vulgare var. hirtum (O) and Coridothymus capitatus (C) essential oils (EOs) and hydrolates (HYs) of the same botanical species was evaluated on sixteen L. monocytogenes strains from food and clinical origins. The antimicrobial activity was assessed by Minimum Inhibitory Concentration (MIC) determination, viable cell enumeration over time up to 60 min, and evaluation of the cellular damage through Confocal Laser Scanning Microscope (CLSM) analysis. EOs exhibited antimicrobial activity with MIC values ranging from 0.3125 to 10 µL/mL. In contrast, HYs demonstrated antimicrobial effectiveness at higher concentrations (125–500 µL/mL). The effect of HYs was rapid after the contact with the cells, and the cell count reduction over 60 min of HY treatment was about 1.2–1.7 Log CFU/mL. L. monocytogenes cells were stressed by HY treatment, and red cell aggregates were revealed through CLSM observation. Moreover, the combinations of EOs and HYs had an additive antilisterial effect in most cases and allowed the concentration of use to be reduced, while maintaining or improving the antimicrobial effectiveness. The combined use of EOs and HYs can offer novel opportunities for applications, thereby enhancing the antimicrobial effectiveness and diminishing the concentration of use. This provides the added benefit of reducing toxicity and mitigating any undesirable sensory effects. [ABSTRACT FROM AUTHOR]

The utilization of coal ashes to replace different natural aggregates is a standard procedure adopted nowadays for different civil engineering applications. Reutilization of these industrial waste products generated from the thermal power plants for civil engineering applications is beneficial to society and the environment in various ways. Thus, this research paper deals with the studies done to analyse the effectiveness of using pond ash for controlled low strength materials (CLSM) production. Further, stresses and strains developed on the buried pipelines where CLSM was used instead of the standard aggregate-based bedding layer were also analysed. A comparative study of the stresses and strains developed on the buried pipelines was evaluated using PLAXIS 2D software. The results from the studies showed that the plastic and in-service properties obtained for the pond ash-based CLSM mixtures are equivalent to regular aggregates used in bedding layer applications. Further, the analysis of stresses and strains developed on the buried pipelines using the Plaxis 2D numerical tool showed that the stresses and strains developed on CLSM as bedding layer-based pipelines performed better than the normal bedding layers. Thus, pond ash-based CLSM mixtures can be used as an alternative to normal aggregates as the stresses and strains developed were found to be lesser than that of normal aggregatebased bedding layers. [ABSTRACT FROM AUTHOR]

Copyright of Journal der Deutschen Dermatologischen Gesellschaft is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Summary: Background and objectives: Onychomycosis is common and important to distinguish from other nail diseases. Rapid and accurate diagnosis is necessary for optimal patient treatment and outcome. Non‐invasive diagnostic tools have increasing potential for nail diseases including onychomycosis. This study evaluated line‐field confocal optical coherence tomography (LC‐OCT) as a rapid non‐invasive tool for diagnosing onychomycosis as compared to confocal laser scanning microscopy (CLSM), optical coherence tomography (OCT), and conventional methods. Patients and Methods: In this prospective study 86 patients with clinically suspected onychomycosis and 14 controls were examined using LC‐OCT, OCT, and CLSM. KOH‐preparation, fungal culture, PCR, and histopathology were used as comparative conventional methods. Results: LC‐OCT had the highest sensitivity and negative predictive value of all methods used, closely followed by PCR and OCT. Specificity and positive predictive value of LC‐OCT were as high as with CLSM, while OCT scored much lower. The gold standard technique, fungal culture, showed the lowest sensitivity and negative predictive value. Only PCR and culture allowed species differentiation. Conclusions: LC‐OCT enables quick and non‐invasive detection of onychomycosis, with advantages over CLSM and OCT, and similar diagnostic accuracy to PCR but lacking species differentiation. For accurate nail examination, LC‐OCT requires well‐trained and experienced operators. [ABSTRACT FROM AUTHOR]

Understanding the rheological behavior of highly concentrated proteins is critical for the development of meat analogs. To enhance their sensory appeal, plant oil is often added during extrusion. The objective of this study was to gain a better understanding of how oil affects the rheological properties of gluten. Gluten doughs with different oil contents (0% to 6%) were prepared by adding emulsified and non-emulsified oil. Rheological measurements were performed under extrusion-relevant conditions using a closed cavity rheometer. In addition, the oil phase was visualized by CLSM imaging before and after rheological measurements. It was found that the complex modulus |G*| of the wheat gluten decreased with increasing oil concentration. When oil was added as emulsion, |G*| was significantly higher compared to when non-emulsified oil was added. The CLSM images revealed that the oil droplet size increased with increasing oil concentration. The doughs with emulsified oil had significantly smaller oil droplets. The results suggest that oil droplets act as fillers, weakening the protein matrix. By reducing the oil droplet size, this weakening effect could be reduced. Therefore, we concluded that the oil droplet size is a critical parameter influencing the rheological properties of oil-containing wheat gluten doughs under extrusion-relevant conditions.

Pilostyles, an endoparasitic genus within the Apodanthaceae family, grows inside host stems with flowers and fruits being the only external manifestations. Previous studies of P. berteroi growing on Adesmia trijuga provided limited details of the endophyte and omitted the origin of flowers and sinker structure. This study, using classical methods of optical microscopy applied to the analysis with scanning electron microscopy and confocal laser scanning microscopy, expands the understanding of the P. berteroi/A. trijuga complex. We find that P. berteroi develops isophasically with its host, forming endophytic patches between the host’s secondary phloem cells. The parasitized Adesmia stem’s cambium primarily produces xylem parenchyma, with limited vessel production and halting fiber formation. The radial polarization of endophytic patches led to the formation of floral meristems. Flowers develop endogenously and emerge by the breakthrough of the host stem. Flowers are connected to the host cambium via chimeric sinkers, combining P. berteroi parenchyma and tracheoids with Adesmia vessels. Unlike previous studies that show uniformity among Pilostyles species, our analysis reveals new insights into the structural interaction between P. berteroi and A. trijuga.

The goal of this investigation is to improve the topical delivery of medicine by preparing and maximizing the potential of a nanotransferosome gel infused with Solanum xanthocarpum methanolic extract (SXE) to provide localized and regulated distribution. Thin-film hydration was used to create SXE-infused nanotransferosomes (SXE-NTFs), and a Box–Behnken design was used to improve them. Phospholipon 90G (X1), cholesterol (X2) and sodium cholate (X3) were chosen as the independent variables, and their effects on vesicle size (Y1), polydispersity index (PDI) (Y2) and the percentage of entrapment efficiency (EE) (Y3) were observed both individually and in combination. For the SXE-NTFs, the vesicle size was 146.3 nm, the PDI was 0.2594, the EE was 82.24 ± 2.64%, the drug-loading capacity was 8.367 ± 0.07% and the drug release rate was 78.86 ± 5.24%. Comparing the antioxidant activity to conventional ascorbic acid, it was determined to be 83.51 ± 3.27%. Ex vivo permeation tests revealed that the SXE-NTF gel (82.86 ± 2.38%) considerably outperformed the SXE gel (35.28 ± 1.62%) in terms of permeation. In addition, it seemed from the confocal laser scanning microscopy (CLSM) picture of the Wistar rat's skin that the rhodamine-B-loaded SXE-NTF gel had a higher penetration capability than the control. Dermatokinetic studies showed that the SXE-NTF gel had a better retention capability than the SXE gel. According to the experimental results, the SXE-NTF gel is a promising and successful topical delivery formulation. [ABSTRACT FROM AUTHOR]

Background: The specificity in the detection of atypical and malignant melanocytic lesions in the context of skin cancer screening is very low, so that numerous unnecessary operations are performed. The extent to which complementary diagnosis using confocal laser scanning microscopy (CLSM, RCM) in skin cancer diagnosis can reduce the number of excisions is investigated. Patients and Methods: Prospective real-world study conducted in an outpatient dermatological practice. Out of 521 consecutive patients with an average age of 56 years, 173 atypical melanocytic lesions were examined using a three-step diagnostic chain: clinical examination, digital dermatoscopy, and confocal laser scanning microscopy. Results: After supplementary confocal microscopic examination, only 18 of the 173 suspicious melanocytic lesions were excised. 89.6 % of the previously planned excisions could be saved. 12 of the 18 excised lesions were dysplastic, a Spitz nevus or a melanoma in situ in the dermatohistopathological findings. Conclusion: By reducing skin surgeries in our study by nearly 90%, it was demonstrated that patients in an outpatient dermatological practice significantly benefit from the additional pain-free, imaging histological diagnostics at the cellular level using Confocal Laser Scanning Microscopy in skin cancer early detection. This diagnostic method needs to be learned and could be widely used for a substantially improved skin cancer screening. [ABSTRACT FROM AUTHOR]

This paper provides a comprehensive revision of the Palearctic plant bug genus Anapus Stål, 1858. Despite a limited number of included species, identification in this group remains challenging due to significant intraspecific polymorphism in the coloration and body proportions. To tackle this issue, confocal microscopy was used to study the male genitalia, and three-dimensional reconstructions of the aedeagus were obtained for all valid species. Our results reveal advantages of these techniques in visualizing small and complex genital structures over traditional methods of taxonomic imaging. A detailed study of the male and female genitalia resulted in finding novel characters allowing for unequivocal species recognition. The differential diagnosis for the genus and a key to all species are given. Habitus photographs, illustrations of male genitalic structures, scanning micrographs of morphological structures, host and distributional information are provided for all species. Anapus longicornis Jakovlev, 1882 is synonymized with Anapus pachymerus (Reuter, 1881) and a lectotype for the latter species is designated. Anapus americanus Knight, 1959 is transferred to the genus Myrmecophyes Fieber, 1870. [ABSTRACT FROM AUTHOR]

Green tomato (Physalis ixocarpa) is a specie native to Mexico, and it is known as "tomatillo" or "husk tomato". The fruit contains vitamins, minerals, phenolic compounds, and steroidal lactones, presenting antimicrobial activity and antinarcotic effects. Therefore, it is not only used in traditional Mexican cuisine, but also in traditional medicine to relieve some discomforts such as fever, cough, and amygdalitis. However, it is a perishable fruit whose shelf life is very short. As a part of the peel, cuticle, and epicuticular waxes represent the most important part in plant protection, and the specific composition and structural characterization are significant to know how this protective biopolymer keeps quality characteristics in fresh fruits. P. ixocarpa cutin was obtained by enzymatic treatments (cellulase, hemicellulose, and pectinase) and different concentrations of TFA, and studied through Cross Polarization Magic Angle Spinning Nuclear Magnetic Resonance (CPMAS 13C NMR), Ultra-High Performance Liquid Chromatography coupled to Mass Spectrometry (UHPLC-MS), and was morphologically characterized by Confocal Laser Scanning Microscopy (CLSM) and Scanning Electron Microscopy (SEM). The main constituents identified under the basis of UHPLC-MS analysis were 9,10,18-trihydroxy-octadecanoic acid and 9,10-epoxy-18-hydroxy-octadecanoic acid with 44.7 and 37.5%, respectively. The C16 absence and low occurrence of phenolic compounds, besides the presence of glandular trichomes, which do not allow a continuous layer on the surface of the fruit, could be related to a lower shelf life compared with other common fruits such as tomato (Solanum lycopersicum). [ABSTRACT FROM AUTHOR]

Elimination of microbes in the root canal system is crucial for achieving long-term success in endodontic treatment. Further efforts in study design and standardization are needed in order to improve the validity and comparability of in vitro results on endodontic disinfection procedures, in turn improving clinical outcomes. This study optimizes two models at all steps: tooth selection, pretreatment, inoculation method (by growth or centrifugation), and confocal laser scanning microscopy (CLSM)-guided imaging of LIVE/DEAD-stained specimens. Individual anatomical conditions lead to substantial differences in penetration depth. Sclerosis grading (SCG), a classification system introduced in this study, provides information about the sclerosis status of the dentine and is helpful for careful, specific, and comparable tooth selection in in vitro studies. Sonically activated EDTA for the pretreatment of roots, inoculation of Enterococcus faecalis in an overflow model, 3–4 weeks of incubation, as well as polishing of dentine slices before staining, led to advances in the visualization of bacterial penetration and irrigation depths. In contrast, NaOCl pretreatment negatively affected performance reproducibility and should be avoided in any pretreatment. Nonsclerotized teeth (SCG0) can be used for microbial semilunar-shaped inoculation by centrifugation as a "quick-and-dirty" model for initial orientation. In conclusion, CLSM-guided imaging for quantifying endodontic infection/disinfection is a very powerful method after the fine-tuning of materials and methods. [ABSTRACT FROM AUTHOR]

During blending between the binders in hot mix recycling, two physical processes occur, namely blending due to diffusion and blending due to mechanical agitation. In the present study, an experimental approach is developed to observe these processes directly on the laboratory recycled asphalt mix samples. Atomic Force Microscopy (AFM) and Confocal Laser Scanning Microscopy (CLSM) are used as the probing tools. The binder concentration profile is estimated at different locations around the reclaimed asphalt pavement material (RAPM) aggregate. Diffusion is observed to be predominant in some of these locations, while, in some other locations, mechanical mixing is observed to be predominant. Assuming that pure Fickian diffusion is happening at those diffusion-spots, the coefficient of diffusion is estimated from the concentration profile at those locations. It is envisaged that the proposed approach would provide a microscopic perspective of the extent of blending during the recycling process. [ABSTRACT FROM AUTHOR]

Pilidia from the recurvatum group are usually sock-like larvae and include the following types: pilidium recurvatum, pilidium incurvatum, and pilidium prorecurvatum. The origin of these pilidia remains debated.The external morphology, musculature, and the nervous system of pilidium incurvatum from Nha Trang Bay are described using light and confocal laser scanning microscopy. The morphologies of p. recurvatum and p. incurvatum are not significantly different. Unlike pilidium prorecurvatum, p. incurvatum has a telotroch, two ventral nerves, and a double ventral muscle strand; the nerve and the muscular rings are located close to the telotroch. The Dawydoff's hypothesis about the origin of pilidia ex gr. recurvatum from a typical hat-like pilidium is supported. [ABSTRACT FROM AUTHOR]

The diversity of insects can be explained by their ability to fill various ecological niches, which includes the foraging from diverse sources. The cuticle-based feeding structures interact with the food and show adaptations in shape, material composition and mechanical properties to it. In this study, we focus on the mouthparts of a very prominent ambush predator, the antlion larvae of Euroleon nostras. By nanoindentation, we tested the hardness and the Young's modulus of the mouthparts, which are significantly harder and stiffer than other insect cuticle structures. To gain insight into the origins of the high values, we studied the degree of tanning using confocal laser scanning microscopy. Additionally, we determined the content of transition and alkaline earth metals by energy dispersive X-ray spectroscopy. We found that the proportions of Zn, Mn, Fe, Cu, Ca, Mg, and Si correlate with the mechanical property values. We also conducted experiments on the breaking stress, the puncturing and biomechanical behaviour of the jaws, which highlighted their extraordinary strength. These findings are not only valuable for biologists, but also for material scientists, as they contribute to our understanding of the origins of mechanical property heterogeneities in insect cuticle. [ABSTRACT FROM AUTHOR]

Current research focuses on explicitly developing and evaluating nanostructured lipidic carriers (NLCs) for the chemotherapeutic drug Ribociclib (RCB) via the topical route to surmount the inherent bioavailability shortcomings. The absolute oral bioavailability has not been determined, but using a physiologically based pharmacokinetic model it was predicted that 65.8 % of the standard dose of RCB (600 mg) would be absorbed mainly in the small intestine. RCB-NLCs were produced using the solvent evaporation method, and Box-Behnken Design (BBD) was employed to optimize composition. The prepared NLCs had an average PS of 79.29 ± 3.53 nm, PDI of 0.242 ± 0.021, and a %EE of 86.07 ± 3.14. The TEM analysis disclosed the spherical form and non-aggregative nature of the NLCs. The outcomes of an in-vitro release investigation presented cumulative drug release of 84.97 ± 3.37 % in 24 h, significantly higher than that from the RCB suspension (RCB-SUS). Ex-vivo skin permeation investigations on rodent (Swiss albino mice) revealed that RCB-NLCs have 1.91 times increases in skin permeability comparable to RCB-SUS. Compared to RCB-SUS, RCB-NLCs were able to penetrate deeper into the epidermis membrane than RCB-SUS as per the findings of confocal microscopy. In dermatokinetic study, higher amount of RCB was maintained in both the layers of mice's skin when treated with RCB-NLCs gel comparable to the RCB-SUS gel preparation. The in-vitro, ex-vivo, CLSM, and dermatokinetics data demonstrated a significant possibility for this novel RCB formulation to be effective against skin cancer.

Abstract Background The process of copulation in Lepidoptera is understudied and poorly understood from a functional perspective. The purpose of the present paper is to study the interaction of the male and female genitalia of Tortrix viridana Linnaeus, 1758 via three-dimensional models of pairs fixed during copulation. Other techniques (confocal laser scanning microscopy, scanning electron microscopy and histology) were used to clarify the role of the organs involved in the process. Results Three-dimensional models based on micro-CT scanned copulating pairs were generated allowing visualisation of the position of the male and female counterparts, spatial changes during copulation, and the skeleto-muscular apparatus involved in the process. The male genitalia and their musculature are simplified in comparison with other lineages of the family, but the opposite is true for the female genitalia. The attachment of the couple is achieved only through flexion of the valvae, clasping the large and sclerotised sternite 7 of the female. The anal cone and socii of the male are in contact with certain parts of the anal papillae and the sterigma of the female. The long tubular vesica is inserted into the narrow posterior part of the ductus bursae. Its eversion is achieved by an increase in haemolymph pressure. A possible mechanism of stimulation of the female via pulsations of the diverticulum of the vesica was discovered. A compressed sclerotised area of the ductus bursae putatively serves as a valve controlling the transfer of ejaculated materials. Copulation progresses through two phases: in the first the vesica and its diverticulum are inflated by haemolymph, and in the second the diverticulum is not inflated, and the vesica is occupied by viscous ejaculated material. The formation of the multilayered spermatophore was observed, and we discovered that sperm is transferred very late in the copulation process. Conclusions Copulation process in Lepidoptera is studied for the first time with three-dimensional reconstructions of couples of Tortrix viridana, used as a model species. The internal genitalia is the scenario of multiple interactions between male and female, but the external remain static. A possible mechanism of stimulation of the female internal copulation organs is proposed.

Dibothriocephalus ditremus and Dibothriocephalus latus are diphyllobothriidean tapeworms autochthonous to Europe. Their larval stages (plerocercoids) may seriously alter health of their intermediate fish hosts (D. ditremus) or cause intestinal diphyllobothriosis of the final human host (D. latus). Despite numerous data on the internal structure of broad tapeworms, many aspects of the morphology and physiology related to host–parasite co-existence remain unclear for these 2 species. The main objective of this work was to elucidate functional morphology of the frontal part (scolex) of plerocercoids, which is crucial for their establishment in fish tissues and for an early attachment in final hosts. The whole-mount specimens were labelled with different antibodies and examined by confocal microscope to capture their complex 3-dimensional microanatomy. Both species exhibited similar general pattern of immunofluorescent signal, although some differences were observed. In the nervous system, FMRF amide-like immunoreactivity (IR) occurred in the bi-lobed brain, 2 main nerve cords and surrounding nerve plexuses. Differences between the species were found in the structure of the brain commissures and the size of the sensilla. Synapsin IR examined in D. ditremus occurred mainly around FMRF amide-like IR brain lobes and main cords. The unexpected finding was an occurrence of FMRF amide-like IR in terminal reservoirs of secretory gland ducts and excretory canals, which has not been observed previously in any tapeworm species. This may indicate that secretory/excretory products, which play a key role in host–parasite relationships, are likely to contain FMRF amide-related peptide/s.

The Bacillus subtilis strain NDmed was isolated from an endoscope washer-disinfector in a medical environment. NDmed can form complex macrocolonies with highly wrinkled architectural structures on solid medium. In static liquid culture, it produces thick pellicles at the interface with air as well as remarkable highly protruding ‘‘beanstalk-like’’ submerged biofilm structures at the solid surface. Since these mucoid submerged structures are hyper-resistant to biocides, NDmed has the ability to protect pathogens embedded in mixed-species biofilms by sheltering them from the action of these agents. Additionally, this non-domesticated and highly biofilm forming strain has the propensity of being genetically manipulated. Due to all these properties, the NDmed strain becomes a valuable model for the study of B. subtilis biofilms. This review focuses on several studies performed with NDmed that have highlighted the sophisticated genetic dynamics at play during B. subtilis biofilm formation. Further studies in project using modern molecular tools of advanced technologies with this strain, will allow to deepen our knowledge on the emerging properties of multicellular bacterial life.