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[Display omitted] Eosinophils are elusive cells involved in allergic inflammation. Single-cell RNA-sequencing (scRNA-seq) is an emerging approach to deeply characterize cellular properties, heterogeneity, and functionality. We sought to comprehensively characterize the transcriptome and biological functions of human eosinophils at a site of severe allergic inflammation in the esophagus (ie, eosinophilic esophagitis [EoE]). We employed a gravity-based scRNA-seq methodology to sequence blood eosinophils from patients with EoE and control individuals compared to a reanalyzed public scRNA-seq dataset of human esophageal eosinophils of EoE patients. We used flow cytometry, immunostaining, and a stimulation assay to verify mRNA findings. In total, scRNA-seq was obtained from 586 eosinophils (188 from blood [n = 6 individuals] and 398 from esophagus [n = 6 individuals]). The esophageal eosinophils were composed of a population of activated eosinophils (enriched in 659 genes compared with peripheral blood–associated eosinophils) and a small population of eosinophils resembling peripheral blood eosinophils (enriched in 62 genes compared with esophageal eosinophils). Esophageal eosinophils expressed genes involved in sensing and responding to diverse stimuli, most notably IFN-γ, IL-10, histamine and leukotrienes, and succinate. Esophageal eosinophils were most distinguished from other esophageal populations by gene expression of the receptors CCR3 , HRH4, SUCNR1, and VSTM1 ; transcription factors CEBPE, OLIG1, and OLIG2 ; protease PRSS33 ; and the hallmark eosinophil gene CLC. A web of bidirectional eosinophil interactions with other esophageal populations was derived. Comparing esophageal eosinophils and mast cells revealed that esophageal eosinophils expressed genes involved in DNAX-activation protein-12 (also known as TYROBP) interactions, IgG receptor-triggered events, immunoregulation, and IL-10 signaling. In EoE, esophageal eosinophils exist as 2 populations, a minority population resembling blood eosinophils and the other population characterized by high de novo transcription of diverse sensing receptors and inflammatory mediators readying them to potentially intersect with diverse cell types. [ABSTRACT FROM AUTHOR]

Abstract Background This study was conducted to find the best concentration of cholesterol-loaded cyclodextrin (CLC) which has a positive impact on canine post thaw semen quality. Three different concentrations of CLC (0.83 mg/ml; 1.66 mg/ml; 3.32 mg/ml) and 2-hydroxylpropyl-beta-cyclodextrin (HBCD) (1.66 mg/ml) were used in addition to cryopreservation extender and compared with the control after thawing. Samples were assessed using computer-assisted semen analyzer (CASA), flow cytometry, fluorimeter by measuring the fluorescence anisotropy (ANISO) and determining the generalized membrane polarization (GP). Results An addition of 0.83 mg/ml CLC significantly increased the percentage of progressive motile (PROG) and rapid spermatozoa (RAP) (P

Nematic chiral liquid crystals (CLCs) are characterized by a helical arrangement of nematic LC molecules. A layer of CLC typically exhibits an optical reflection band due to Bragg reflection in the helical structure. When several layers of CLC are spin-coated and polymerized on top of each other without a barrier layer in between, defect modes can form in their reflection spectrum. By comparing experimental results and simulations, we investigate the origin of the defect modes, thereby revealing details on the behavior of the materials at the interfaces during deposition. Simulations show that these defect modes can originate from the migration of chiral dopant leading to a layer with a smaller pitch or from a discontinuity in the director orientation at the interface between two layers. [ABSTRACT FROM AUTHOR]

ABSTRACTMicroorganisms resist fluoride toxicity using fluoride export proteins from one of several different molecular families. Cariogenic species Streptococcus mutans and Candida albicans extrude intracellular fluoride using a CLCF F-/H+ antiporter and FEX fluoride channel, respectively, whereas oral commensal eubacteria, such as Streptococcus gordonii, export fluoride using a Fluc fluoride channel. In this work, we examine how genetic knockout of fluoride export impacts pathogen fitness in single-species and three-species dental biofilm models. For biofilms generated using S. mutans with the genetic knockout of the CLCF transporter, exposure to low fluoride concentrations decreased S. mutans counts, synergistically reduced the populations of C. albicans, increased the relative proportion of oral commensal S. gordonii, and reduced properties associated with biofilm pathogenicity, including acid production and hydroxyapatite dissolution. Biofilms prepared with C. albicans with genetic knockout of the FEX channel also exhibited reduced fitness in the presence of fluoride but to a lesser degree. Imaging studies indicate that S. mutans is highly sensitive to fluoride, with the knockout strain undergoing complete lysis when exposed to low fluoride for a moderate amount of time. Biochemical purification of the S. mutans CLCF transporter and functional reconstitution establishes that the functional protein is a dimer encoded by a single gene. Together, these findings suggest that fluoride export by oral pathogens can be targeted by specific inhibitors to restore biofilm symbiosis in dental biofilms and that S. mutans is especially susceptible to fluoride toxicity.IMPORTANCEDental caries is a globally prevalent condition that occurs when pathogenic species, including Streptococcus mutans and Candida albicans, outcompete beneficial species, such as Streptococcus gordonii, in the dental biofilm. Fluoride is routinely used in oral hygiene to prevent dental caries. Fluoride also has antimicrobial properties, although most microbes possess fluoride exporters to resist its toxicity. This work shows that sensitization of cariogenic species S. mutans and C. albicans to fluoride by genetic knockout of fluoride exporters alters the microbial composition and pathogenic properties of dental biofilms. These results suggest that the development of drugs that inhibit fluoride exporters could potentiate the anticaries effect of fluoride in over-the-counter products like toothpaste and mouth rinses. This is a novel strategy to treat dental caries.

Ecosystems provide various goods and services to society and their valuation is among the main objectives of the concept of ecosystem services (ES). The mapping of ecosystems is the main building block of the whole process of the Mapping and Assessment of Ecosystems and their Services (MAES). The analyses of the ecosystem data produced during the implementation of the national methodology for mapping ecosystems in Bulgaria (MAES BG) reveal some problems that may cause confusion in cases of integrated assessment of all ecosystem types. In this paper, we present an approach that enables formulation of a uniform spatial dataset based on the mapping of the main ecosystem types, that can be used for mapping of ES at a river basin scale. It has been applied to the upper part of the Ogosta River basin and the result is a topologically correct uniform spatial data layer. The approach gives one possible solution to problems related to the different sources of information and the discrepancies between ecosystem types in the national mapping of ecosystems in Bulgaria. It is based on the use of a uniform spatial framework that outlines the ecosystem types and sets the initial database for further mapping. This ensures a topologically correct spatial dataset for the ecosystems and a background for further updates for each ecosystem at the different levels of MAES typology. The most appropriate spatial basis for the territory of Bulgaria is the database for the physical blocks of the Ministry of Interior. Its application to the studied river basin gives encouraging results and can be used as an example for similar areas. Further development of the approach will ensure the mapping of the forest ecosystems at level 3 of the MAES BG typology and more precise delineation of the grassland, heathland, freshwater, and sparsely vegetated ecosystems.

Bata ringan berdimensi seragam dan berukuran lebih besar daripada bata merah, bata ringan memiliki ketahanan sekuat beton dan bobot bata ringan juga lebih ringan daripada bata merah serta beton biasa. Proses penambahan gelembung udara ke campuran semen, air dan agregat halus akan menghasilkan bata ringan. Penambahan gelembung udara tersebut mengurangi berat bata yang dihasilkan. Bata ringan terbagi menjadi dua jenis, yaitu Autoclaved Aerated Concrete (AAC) dan Cellular Lightweight Concrete (CLC). Harga bata ringan berjenis CLC lebih hemat jika dibandingkan dengan bata ringan berjenis AAC. Fly ash merupakan abu terbang hasil pembakaran. Fly ash adalah partikel halus yang bersifat pozzolan. Reaksi hidrasi semen dan air akan menyebabkan fly ash berubah bentuk dari partikel halus menjadi massa padat. Penelitian ini memiliki tujuan untuk memperoleh manfaat dan pengaruh fly ash batu bara sebagai substitusi sebagian semen terhadap nilai kuat tekan bata ringan berjenis CLC. Metode penelitian yang digunakan adalah metode eksperimen yang mensubstitusikan sebagian semen menggunakan fly ash batu bara. Variasi persentase substitusinya terdiri dari 0%, 20%, 30%, 40% dan 50% terhadap berat semen. Bata ringan dilakukan pengujian kuat tekan ketika umur perawatan telah mencapai 28 hari. Hasil penelitian kuat tekan bata ringan berjenis CLC memperoleh nilai kuat tekan maksimum, yaitu 8,539 MPa dengan variasi fly ash 30% dan memperoleh nilai kuat tekan minimum, yaitu 5,351 MPa dengan variasi fly ash 50%. Berdasarkan hasil penelitian, nilai persentase optimum fly ash batu bara sebagai substitusi sebagian semen terhadap nilai kuat tekan maksimum bata ringan berjenis CLC diperoleh pada variasi 30%.

ClC‐6 and ClC‐7 are closely related, intracellular Cl−/H+ antiporters belonging to the CLC family of channels and transporters. They localize to acidic late endosomes and lysosomes and probably function in ionic homeostasis of these contiguous compartments. ClC‐7 transport function requires association with the accessory protein Ostm1, whereas ClC‐6 transport does not. To elucidate their roles in endo‐lysosomes, we measured Cl−‐ and pH‐dependences of over‐expressed wild‐type ClC‐6 and ClC‐7, as well as disease‐associated mutants, using high‐resolution recording protocols. Lowering extracellular Cl− (corresponding to luminal Cl− in endo‐lysosomes) reduced ClC‐6 currents, whereas it increased transport activity of ClC‐7/Ostm1. Low extracellular Cl− activated ClC‐7/Ostm 1 under acidic extracellular conditions, as well as under conditions of low intracellular chloride. Activation is conserved in ClC‐7Y713C, a variant displaying disrupted PI(3,5)P2 inhibition. Detailed biophysical analysis of disease‐associated ClC‐6 and ClC‐7 gain‐of‐function (GoF) variants, ClC‐6Y553C and ClC‐7Y713C, and the ClC‐7Y577C and ClC‐6Y781C correlates, identified additional functional nuances distinguishing ClC‐6 and ClC‐7. ClC‐7Y577C recapitulated GoF produced by ClC‐6Y553C. ClC‐6Y781C displayed transport activation qualitatively similar to ClC‐7Y713C, although current density did not differ from that of wild‐type ClC‐6. Finally, rClC‐7R760Q, homologous to hClC‐7R762Q, an osteopetrosis variant with fast gating kinetics, appeared indifferent to extracellular Cl−, identifying altered Cl− sensitivity as a plausible mechanism underlying disease. Collectively, the present studies underscore the distinct roles of ClC‐6 and ClC‐7 within the context of their respective localization to late endosomes and lysosomes. In particular, we suggest the atypical inhibition of ClC‐7 by luminal Cl− serves to limit excessive intraluminal Cl− accumulation. Key points: ClC‐6 and ClC‐7 are late endosomal and lysosomal 2 Cl−/1 H+ exchangers, respectively. When targeted to the plasma membrane, both activate slowly at positive voltages.ClC‐6 activity is decreased in low extracellular (i.e. luminal) chloride, whereas ClC‐7 is activated by low luminal chloride, even at acidic pH.The functional gain‐of‐function phenotypes of the ClC‐6 and ClC‐7 disease mutations ClC‐6Y553C and ClC‐7Y715C are maintained when introduced in their respective homologues, ClC‐7Y577C and ClC‐6Y781C, with all mutations retaining chloride dependence of the respective wild type (WT).An osteopetrosis mutation of ClC‐7 displaying fast gating kinetics (R762Q) was less sensitive to extracellular chloride compared to WT.The opposing substrate dependences of ClC‐6 and ClC‐7 Cl− / H+ exchangers point to non‐overlapping physiological functions, leading us to propose that inhibition of ClC‐7 by luminal chloride and protons serves to prevent osmotic stress imposed by hyper‐accumulation of chloride. [ABSTRACT FROM AUTHOR]

While the Protein Data Bank (PDB) contains a wealth of structural information on ligands bound to macromolecules, their analysis can be challenging due to the large amount and diversity of data. Here, we present PDBe CCDUtils, a versatile toolkit for processing and analysing small molecules from the PDB in PDBx/mmCIF format. PDBe CCDUtils provides streamlined access to all the metadata for small molecules in the PDB and offers a set of convenient methods to compute various properties using RDKit, such as 2D depictions, 3D conformers, physicochemical properties, scaffolds, common fragments, and cross-references to small molecule databases using UniChem. The toolkit also provides methods for identifying all the covalently attached chemical components in a macromolecular structure and calculating similarity among small molecules. By providing a broad range of functionality, PDBe CCDUtils caters to the needs of researchers in cheminformatics, structural biology, bioinformatics and computational chemistry. [ABSTRACT FROM AUTHOR]

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The CLC-ec1 chloride/proton antiporter is a membrane-embedded homodimer with subunits that can dissociate and associate, but the thermodynamic driving forces favor the assembled dimer at biological densities. Yet, the physical reasons for this stability are confounding as dimerization occurs via the burial of hydrophobic interfaces away from the lipid solvent. For binding of nonpolar surfaces in aqueous solution, the driving force is often attributed to the hydrophobic effect, but this should not apply in the membrane since there is very little water. To investigate this further, we quantified the thermodynamic changes associated with CLC dimerization in membranes by carrying out a van ‘t Hoff analysis of the temperature dependency of the free energy of dimerization, ΔG°. To ensure that the reaction reached equilibrium at different temperatures, we utilized a Förster resonance energy transfer assay to report on relaxation kinetics of subunit exchange as a function of temperature. Equilibration times were then applied to measure CLC-ec1 dimerization isotherms at different temperatures using the single-molecule subunit-capture photobleaching analysis approach. The results demonstrate that the dimerization free energy of CLC in Escherichia coli–like membranes exhibits a nonlinear temperature dependency corresponding to a large, negative change in heat capacity, a signature of solvent ordering effects such as the hydrophobic effect. Consolidating this with our previous molecular analyses suggests that the nonbilayer defect required to solvate the monomeric state is one source of the observed change in heat capacity and indicates the existence of a generalizable driving force for protein association in membranes. [ABSTRACT FROM AUTHOR]

The tfd (tfdI and tfdII) are gene clusters originally discovered in plasmid pJP4 which are involved in the bacterial degradation of 2,4-dichlorophenoxyacetic acid (2,4-D) via the ortho-cleavage pathway of chlorinated catechols. They share this activity, with respect to substituted catechols, with clusters tcb and clc. Although great effort has been devoted over nearly forty years to exploring the structural diversity of these clusters, their evolution has been poorly resolved to date, and their classification is clearly obsolete. Employing comparative genomic and phylogenetic approaches has revealed that all tfd clusters can be classified as one of four different types. The following four-type classification and new nomenclature are proposed: tfdI, tfdII, tfdIII and tfdIV(A,B,C). Horizontal gene transfer between Burkholderiales and Sphingomonadales provides phenomenal linkage between tfdI, tfdII, tfdIII and tfdIV type clusters and their mosaic nature. It is hypothesized that the evolution of tfd gene clusters proceeded within first (tcb, clc and tfdI), second (tfdII and tfdIII) and third (tfdIV(A,B,C)) evolutionary lineages, in each of which, the genes were clustered in specific combinations. Their clustering is discussed through the prism of hot spots and driving forces of various models, theories, and hypotheses of cluster and operon formation. Two hypotheses about series of gene deletions and displacements are also proposed to explain the structural variations across members of clusters tfdII and tfdIII, respectively. Taking everything into account, these findings reconstruct the phylogeny of tfd clusters, have delineated their evolutionary trajectories, and allow the contribution of various evolutionary processes to be assessed. [ABSTRACT FROM AUTHOR]

Ecosystems provide various goods and services to society and their valuation is among the main objectives of the concept of ecosystem services (ES). The mapping of ecosystems is the main building block of the whole process of the Mapping and Assessment of Ecosystems and their Services (MAES). The analyses of the ecosystem data produced during the implementation of the national methodology for mapping ecosystems in Bulgaria (MAES BG) reveal some problems that may cause confusion in cases of integrated assessment of all ecosystem types. In this paper, we present an approach that enables formulation of a uniform spatial dataset based on the mapping of the main ecosystem types, that can be used for mapping of ES at a river basin scale. It has been applied to the upper part of the Ogosta River basin and the result is a topologically correct uniform spatial data layer. The approach gives one possible solution to problems related to the different sources of information and the discrepancies between ecosystem types in the national mapping of ecosystems in Bulgaria. It is based on the use of a uniform spatial framework that outlines the ecosystem types and sets the initial database for further mapping. This ensures a topologically correct spatial dataset for the ecosystems and a background for further updates for each ecosystem at the different levels of MAES typology. The most appropriate spatial basis for the territory of Bulgaria is the database for the physical blocks of the Ministry of Interior. Its application to the studied river basin gives encouraging results and can be used as an example for similar areas. Further development of the approach will ensure the mapping of the forest ecosystems at level 3 of the MAES BG typology and more precise delineation of the grassland, heathland, freshwater, and sparsely vegetated ecosystems. [ABSTRACT FROM AUTHOR]

Currently, there is a lack of detailed knowledge about methane (CH4) reactions on metal oxide surfaces. This reaction involves in different processes such as chemical looping combustion and catalytic carbon dioxide (CO2) reforming of CH4. In this study, in situ diffuse reflectance infrared Fourier transform spectroscopy was used to investigate CH4 reaction on three transition metal oxides (CoO, CuO, and α-Fe2O3) which are commonly used in these processes at temperatures ranging from 100 °C to 700 °C. The results show that CH4 is adsorbed on the metal oxide surfaces as methoxy and formate, and then the methoxy group reacts with the metal oxide lattice oxygen forming bicarbonate. Under the effect of the used reaction temperature, the bicarbonate decomposes to CO2 and water vapor. [ABSTRACT FROM AUTHOR]

Background: Filarial infections continue to pose a great challenge in endemic countries. One of the central goals in the fight against human filarial infections is the development of strategies that will lead to the inhibition of microfilariae (mf) transmission. Keeping mf under a certain threshold within endemic populations will stop transmission and eliminate the infection. Method: A narrative review was carried out to identify the possibilities and limitations of exploring the use of eosinophil responses as an anti‐filarial vaccine, and biomarker for the detection of filarial infections. An extensive literature search was performed in online scientific databases including PubMed Central, PubMed, BioMed Central, with the use of predefined search terms. Results: A better understanding of the parasite‐host interactions will lead to the development of improved and better treatment or vaccine strategies that could eliminate filariasis as soon as possible. Highlighted in this review is the explorative use of eosinophil‐producing CLC/Galectin‐10 as a potential biomarker for filarial infections. Also discussed are some genes, and pathways involved in eosinophil recruitments that could be explored for anti‐filarial vaccine development. Conclusion: In this short communication, we discuss how eosinophil‐regulated genes, pathways, and networks could be critical in providing more information on how reliably a front‐line immune player could be exploited for anti‐filarial vaccine development and early infection biomarker. [ABSTRACT FROM AUTHOR]

Increased computing power has made it possible to run simulations of the Weather Research and Forecasting (WRF) numerical model in high spatial resolution. However, running high-resolution simulations requires a higher-detail mapping of landforms, land use, and land cover. Often, higher-resolution data have limited coverage or availability. This paper presents the feasibility of using CORINE Land Cover (CLC) land use and land cover data and alternative high-resolution global coverage land use/land cover (LULC) data from Copernicus Global Land Service Land Cover Map (CGLS-LC100) V2.0 in high-resolution WRF simulations (100 × 100 m). Global LULC data with a resolution of 100 m are particularly relevant for areas not covered by CLC. This paper presents the method developed by the authors for reclassifying land cover data from CGLS-LC100 to MODIS land use classes with defined parameters in the WRF model and describes the procedure for their implementation into the model. The obtained simulation results of the basic meteorological parameters from the WRF simulation using CLC, CGLS-LC100 and default geographical data from MODIS were compared to observations from 13 meteorological stations in the Warsaw area. The research has indicated noticeable changes in the forecasts of temperature, relative humidity wind speed, and direction after using higher-resolution LULC data. The verification results show a significant difference in weather predictions in terms of CLC and CGLS-LC100 LULC data implementation. Due to the fact that better results were obtained for CLC simulations than for CGLS-LC100, it is suggested that CLC data are first used for simulations in numerical weather prediction models and to use CGLS-LC100 data when the area is outside of CLC coverage. [ABSTRACT FROM AUTHOR]

Chemical-looping combustion (CLC) is a highly efficient CO2 separation technology with no direct contact between combustion air and fuel. A metal oxide is used as an oxygen carrier (OC) and acts in a dual fluidized bed as a separation tool and supplies the fuel with oxygen, which as an oxidation medium causes combustion to CO2 and H2O. The use of solid fuels, especially biomass, is the focus of current investigations. The OC plays a key role, because it must meet special requirements for solid fuels, which are different to gaseous fuels. The ash content, special reaction mechanisms, and increased abrasion make research into new types of OC essential. Preliminary testing of OC before their use in larger plants regarding their suitability is recommended. For this reason, this work shows the design and the results of a laboratory reactor, which was planned and built for fundamental investigation of OC. Designed as a transient fluidized bed, the reactor, equipped with its own fuel conveying system and an in situ solid sampling, is intended to be particularly suitable for cheap and rapid pre-testing of OC materials. During the tests, it was shown that the sampling device enables non-selective sampling. Different OC were tested under various operating conditions, and their ability to convert different fuels could be quantified. The results indicate that OC can be sufficiently investigated to recommend operation in larger plants. [ABSTRACT FROM AUTHOR]

Introduction: Behçet syndrome (BS) is a chronic, multisystemic inflammatory condition with unanswered questions regarding its pathogenesis and rational therapeutics. A microarray‐based comparative transcriptomic analysis was performed to elucidate the molecular mechanisms of BS and identify any potential therapeutic targets. Methods: Twenty‐nine BS patients (B) and 15 age and sex‐matched control subjects (C) were recruited. Patients were grouped as mucocutaneous (M), ocular (O), and vascular (V) according to their clinical phenotypes. GeneChip Human Genome U133 Plus 2.0 arrays were used for expression profiling on peripheral blood samples of the patients and the control subjects. Following documentation of the differentially expressed gene (DEG) sets, the data were further evaluated with bioinformatics analysis, visualization, and enrichment tools. Validation of the microarray data was performed using quantitative reverse transcriptase polymerase chain reaction. Results: When p ≤ 0.05 and fold change ≥2.0 were chosen, the following numbers of DEGs were obtained; B versus C: 28, M versus C: 20, O versus C: 8, V versus C: 555, M versus O: 6, M versus V: 324, O versus V: 142. Venn diagram analysis indicated only two genes, CLEC12A and IFI27, in the intersection of M versus C ∩ O versus C ∩ V versus C. Another noteworthy gene appeared as CLC in the DEG sets. Cluster analyses successfully clustered distinct clinical phenotypes of BS. While innate immunity‐related processes were enriched in the M group, adaptive immunity‐specific processes were significantly enriched in the O and V groups. Conclusions: Distinct clinical phenotypes of BS patients displayed distinct expression profiles. In Turkish BS patients, expression differences regarding the genes CLEC12A, IFI27, and CLC seemed to be operative in the disease pathogenesis. Based on these findings, future research should consider the immunogenetic heterogeneity of BS clinical phenotypes. Two anti‐inflammatory genes, namely CLEC12A and CLC, may be valuable as therapeutic targets and may also help design an experimental model in BS. [ABSTRACT FROM AUTHOR]

This paper reviews various separation techniques used in purification processes to remove pollutants like carbon dioxide and hydrogen sulfide from petroleum products. The most effective techniques include absorption, adsorption, cryogenic distillation, chemical looping combustion, and membrane separation. The study reviews over 100 published studies to assess their characteristics, benefits, and drawbacks. The choice of separation technology depends on ideal conditions, cost, efficiency, and energy required in the regeneration phase.

Nematic chiral liquid crystals (CLCs) are characterized by a helical arrangement of nematic LC molecules. A layer of CLC typically exhibits an optical reflection band due to Bragg reflection in the helical structure. When several layers of CLC are spin-coated and polymerized on top of each other without a barrier layer in between, defect modes can form in their reflection spectrum. By comparing experimental results and simulations, we investigate the origin of the defect modes, thereby revealing details on the behavior of the materials at the interfaces during deposition. Simulations show that these defect modes can originate from the migration of chiral dopant leading to a layer with a smaller pitch or from a discontinuity in the director orientation at the interface between two layers.

The author comments on a study that demonstrates how a mutation in the anion chloride channel ClC-K-barttin affects an endocytic YxxØ motif and uses expression in Xenopus oocytes and mammalian cells to link increased stability of the ClC-K-barttin complex. He discusses the finding that the barttin point mutation left protein expression levels and subcellular distribution unaffected under control conditions. He explains the significance of linking protein functions to organ physiology.

Abstract Background Filarial infections continue to pose a great challenge in endemic countries. One of the central goals in the fight against human filarial infections is the development of strategies that will lead to the inhibition of microfilariae (mf) transmission. Keeping mf under a certain threshold within endemic populations will stop transmission and eliminate the infection. Method A narrative review was carried out to identify the possibilities and limitations of exploring the use of eosinophil responses as an anti‐filarial vaccine, and biomarker for the detection of filarial infections. An extensive literature search was performed in online scientific databases including PubMed Central, PubMed, BioMed Central, with the use of predefined search terms. Results A better understanding of the parasite‐host interactions will lead to the development of improved and better treatment or vaccine strategies that could eliminate filariasis as soon as possible. Highlighted in this review is the explorative use of eosinophil‐producing CLC/Galectin‐10 as a potential biomarker for filarial infections. Also discussed are some genes, and pathways involved in eosinophil recruitments that could be explored for anti‐filarial vaccine development. Conclusion In this short communication, we discuss how eosinophil‐regulated genes, pathways, and networks could be critical in providing more information on how reliably a front‐line immune player could be exploited for anti‐filarial vaccine development and early infection biomarker.

Abstract Introduction Behçet syndrome (BS) is a chronic, multisystemic inflammatory condition with unanswered questions regarding its pathogenesis and rational therapeutics. A microarray‐based comparative transcriptomic analysis was performed to elucidate the molecular mechanisms of BS and identify any potential therapeutic targets. Methods Twenty‐nine BS patients (B) and 15 age and sex‐matched control subjects (C) were recruited. Patients were grouped as mucocutaneous (M), ocular (O), and vascular (V) according to their clinical phenotypes. GeneChip Human Genome U133 Plus 2.0 arrays were used for expression profiling on peripheral blood samples of the patients and the control subjects. Following documentation of the differentially expressed gene (DEG) sets, the data were further evaluated with bioinformatics analysis, visualization, and enrichment tools. Validation of the microarray data was performed using quantitative reverse transcriptase polymerase chain reaction. Results When p ≤ 0.05 and fold change ≥2.0 were chosen, the following numbers of DEGs were obtained; B versus C: 28, M versus C: 20, O versus C: 8, V versus C: 555, M versus O: 6, M versus V: 324, O versus V: 142. Venn diagram analysis indicated only two genes, CLEC12A and IFI27, in the intersection of M versus C ∩ O versus C ∩ V versus C. Another noteworthy gene appeared as CLC in the DEG sets. Cluster analyses successfully clustered distinct clinical phenotypes of BS. While innate immunity‐related processes were enriched in the M group, adaptive immunity‐specific processes were significantly enriched in the O and V groups. Conclusions Distinct clinical phenotypes of BS patients displayed distinct expression profiles. In Turkish BS patients, expression differences regarding the genes CLEC12A, IFI27, and CLC seemed to be operative in the disease pathogenesis. Based on these findings, future research should consider the immunogenetic heterogeneity of BS clinical phenotypes. Two anti‐inflammatory genes, namely CLEC12A and CLC, may be valuable as therapeutic targets and may also help design an experimental model in BS.

Casuarina glauca is an important coastal protection forest species, which is exposed to high salt stress all year round. Arbuscular mycorrhizal fungi (AMF) can promote the growth and salt tolerance of C. glauca under salt stress. However, the effects of AMF on the distribution of Na+ and Cl− and the expression of related genes in C. glauca under salt stress need to be further explored. This study explored the effects of Rhizophagus irregularis on plant biomass, the distribution of Na+ and Cl−, and the expression of related genes in C. glauca under NaCl stress through pot simulation experiments. The results revealed that the mechanisms of Na+ and Cl− transport of C. glauca under NaCl stress were different. C. glauca took a salt accumulation approach to Na+, transferring Na+ from roots to shoots. Salt accumulation of Na+ promoted by AMF was associated with CgNHX7. The transport mechanism of C. glauca to Cl− might involve salt exclusion rather than salt accumulation, and Cl− was no longer transferred to shoots in large quantities but started to accumulate in roots. However, AMF alleviated Na+ and Cl− stress by similar mechanisms. AMF could promote salt dilution of C. glauca by increasing biomass and the content of K+, compartmentalizing Na+ and Cl− in vacuoles. These processes were associated with the expression of CgNHX1, CgNHX2-1, CgCLCD, CgCLCF, and CgCLCG. Our study will provide a theoretical basis for the application of AMF to improve salt tolerance in plants. [ABSTRACT FROM AUTHOR]

Chemical Looping Combustion (CLC) is a technology that efficiently combines power generation and CO2 capture. In CLC, the fuel is oxidized by a metal oxide called an oxygen carrier (OC). CLC uses two reactors: a fuel reactor and an air reactor. The fuel reactor oxidizes the fuel and reduces the OC. The air reactor oxidizes the OC using air and then the OC is cycled back to the fuel reactor. It is typical for both the fuel and the air reactors to be fluidized beds (FBs). In this research, an Aspen Plus model was developed to simulate a CLC system. Aspen Plus has recently included a built-in FB unit operation module. To our knowledge, no literature has been reported using this FB module for simulating fluidized bed combustion or gasification. This FB unit process was investigated in Aspen Plus and a kinetic based model was used and compared the simulation results to experimental data and the commonly used Gibbs equilibrium model. The FB unit and the kinetic model well fit the experimental data for syngas and methane combustion within 2% of the molar composition of syngas combustion and within 4% for the methane combustion. An advantage of this model over other kinetic models in literature is that the core shrinking model kinetic rate equations have been converted into a power law form. This allows Aspen Plus to use a calculator instead of an external Fortran compiler. This greatly simplifies the modeling process. The reaction rate equations are given for all reactions. A sensitivity analysis of the reaction kinetics was conducted. All data, code, and simulation files are given. [ABSTRACT FROM AUTHOR]

Land cover (LC) data incorporation, for weather modeling purposes, highlights many problems. The straightforward Single Level Mode (SLM) aggregation is not adapted for high‐resolution LC maps, with a high number of classes, because it could generate false classifications. We propose a Multi‐Level Mode (MLM) aggregation method that includes a hierarchical structure. This study focuses on the Corine Land Cover (CLC) data set. Differences between MLM and SLM methods are small at the finest horizontal resolution and increase to a value of around 16% at 9‐km horizontal resolution. To further integrate CLC data into WRF (Weather Research Forecasting model), we included a dedicated table of physical parameters next to using the classical conversion toward the USGS one. To evaluate the LC impact on the modeled boundary layer, we used WRF at 1 km to simulate a 4‐day period with diurnal cycles of valley winds in the heterogeneous western pre‐Alps area. We tested three LCs (USGS, MODIS, and CLC), where CLC has two physical parameter tables and two aggregation methods. CLC performs better than other tested LCs. The CLC aggregation methods revealed limited differences between the simulated variables, although the MLM method gives better results. Since these comparisons are restricted to a single location with the same LC type, the differences between various simulations regarding atmospheric parameters probably result from horizontal advection from upwind areas where surface conditions differ. Extended time series with multiple locations and diverse meteorological conditions need to be considered to reinforce the results presented here. Key Points: A new grid aggregation method (MLM) for land use (LU) categories is described, which addresses ambiguities in assigning the LU to cellsSimulations with a physical parameter table specific for the Corine Land Cover (CLC) classes show limited improvements over the conversion to the USGS tableUsing CLC as an LU map improves the Weather Research Forecasting compared to USGS maps [ABSTRACT FROM AUTHOR]

This paper presents the modeling and simulation of an industrial-scale chemical looping combustion (CLC) power plant, including all process units (reactors, flue gas treatment units, heat integration, steam cycle, and CO2 compression train). A model of a 525 MWth CLC power plant was built using a rigorous representation of the solid fuel and oxygen carrier. Petcoke was considered the main fuel of interest in this study, and it is compared with other solid fuels. The flue gas compositions obtained with the model show that cleanup units are mandatory to comply with CO2 quality requirements. High levels of flue gas treatment, including 97.1% deNOx and 99.4% deSOx, are needed to achieve typical specifications for captured CO2. This is mainly due to the high level of contaminants in the fuel, but also to the absence of nitrogen in the CLC flue gas, thus resulting in higher concentrations for all substances. The high level of flue gas treatment is thus one of the important challenges for solid fuel combustion in CLC. The overall CO2 capture efficiency of the plant is estimated to be as high as 94%. Regarding the energy balance, a process net efficiency of 38% is obtained. Comparing the results with other available technologies shows that CLC exhibits one of the highest net plant efficiencies and carbon capture rates. CLC is thus a promising technology to produce clean energy from solid fuels. Finally, based on a sensitivity analysis, it is shown that process efficiency is mainly affected by the design and performance of the CLC furnace, the steam injection rate in the fuel reactor, the char separation efficiency, and the excess oxygen in the air reactor. [ABSTRACT FROM AUTHOR]

• Integrating chemical looping combustion and methane pyrolysis, enabling thermal coupling within the system while achieving only 2.15 kg/MWh CO 2 emission. • Carbon utilization to carbon storage ratio is recommended as 0.68% to realise optimal exergy performance. • This novel process maintains a maximum exergy efficiency of 69.48%. • The relative difference in the environment impact of system is 21.14%, revealing the potential for improving system performance. Natural gas-based energy systems have garnered widespread attention for their potential to facilitate the transition from traditional to renewable energy sources. Among these, methane pyrolysis technology stands out as a promising pathway. Consequently, a novel methanol-electricity-carbon trigeneration system featuring methane pyrolysis coupled with chemical looping combustion, methanol synthesis, and solid oxide fuel cell unit was designed. To validate this innovative concept, thermodynamic model of the process was developed. Compared to conventional single-generation system, the proposed system demonstrated superior thermodynamic performance, with energy efficiency of 69.51 %, representing improvement of 28.78 %. Sensitivity analysis indicated that optimal carbon utilization-to-storage ratio of 0.68 effectively balanced environmental benefits with energy conversion efficiency. Additionally, the CO 2 capture rate and emission of the proposed system reached impressive 99.24 % and 2.15 kg/MWh, respectively, confirming its carbon mitigation potential. Based on this, exergoenvironmental analysis demonstrated that r b , k of the novel system was 21.14 %, indicating room for further environmental impact reduction. Moreover, due to the high r b , k of 16.61 % for the preheater-2 identified as a key component for optimization. Finally, the economic analysis verified the system's feasibility, showing the annual net income of 6774.06 k$. This novel concept highlights the potential of natural gas-based systems in optimizing carbon utilization and energy efficiency, contributing to a more environmentally sustainable energy infrastructure. [ABSTRACT FROM AUTHOR]

CLC channels mediate passive Cl- conduction, while CLC transporters mediate active Cl- transport coupled to H+ transport in the opposite direction. The distinction between CLC-0/1/2 channels and CLC transporters seems undetectable by amino acid sequence. To understand why they are different functionally we determined the structure of the human CLC-1 channel. Its 'glutamate gate' residue, known to mediate proton transfer in CLC transporters, adopts a location in the structure that appears to preclude it from its transport function. Furthermore, smaller side chains produce a wider pore near the intracellular surface, potentially reducing a kinetic barrier for Cl- conduction. When the corresponding residues are mutated in a transporter, it is converted to a channel. Finally, Cl- at key sites in the pore appear to interact with reduced affinity compared to transporters. Thus, subtle differences in glutamate gate conformation, internal pore diameter and Cl- affinity distinguish CLC channels and transporters.

The nutritional enhancement of potato plants (Solanum tuberosum L.,) is highly critical. As it is considered a worldwide basic vegetarian nutrition to maintain health. S. tuberosum is one of the foremost staples and the world's fourth-largest food crop. In advance, its need is increasing because of its high-industrial value and population blast. To improve both potato growth and behavior under harsh environmental conditions, we produced transgenic potato plants overexpressing either VvNHX (a sodium proton antiporter from Vitis vinifera), VvCLC (a chloride channel from Vitis vinifera), or both. Control and transgenic plants were grown in greenhouse and field under non-stressed conditions for 85 days in order to characterize their phenotype and evaluate their agronomical performance. To this aim, the evaluation of plant growth parameters, tuber yields and characteristics (calibers, eye number and color), the chemical composition of tubers, was conducted and compared between the different lines. The obtained results showed that transgenic plants displayed an improved growth (flowering precocity, gain of vigor and better vegetative growth) along with enhanced tuber yields and quality (increased protein and starch contents). Our findings provide then insight into the role played by the VvNHX antiport and the VvCLC channel and a greater understanding of the effect of their overexpression in potato plants. • Transgenic potato plants overexpressing either VvCLC4 or both VvNHX1 and VvCLC4 , were produced in this study and showed different traits. • The genetic transformation significantly improved the agronomic characteristics, i.e., flowering precocity, gain of vigor, vegetative growth and tuber yields in the transgenic lines. • Monotransgenic potato plants overexpressing VvCLC4 and double transgenic potato plants overexpressing VvNHX1 and VvCLC4 showed highest nitrate contents in tubers suggesting that VvCLC4 proteins would be involved in the nitrate transport from the cytoplasm into the vacuole. [ABSTRACT FROM AUTHOR]

Chemical looping combustion (CLC) is a modern technology that enables the mitigation of the CO2 concentration without any expense of energy. Experimental evidence shows that combustion of coal/biomass in CLC technology leads to negative carbon emission by replacing the portion of coal with biomass. In the present work, CLC was simulated using a mixture of coal/biomass in CLC; using their different proportions resulted in enhanced CO and CO2 fractions in the fuel reactor. The carbon capture and oxide oxygen fraction were also found to increase with the enhancement of the fuel reactor's temperature with different proportions of coal/biomass. Increases in the carbon capture efficiency and oxide oxygen fraction of up to 98.86% and ~98%, respectively, were observed within the experimental temperature range. The simulated results of various parameters were predicted and validated with the published experimental results. The stated parameters were also predicted as a function of the different rates of solid circulation and gasification agents. Higher coal char conversion was confirmed in the fuel reactor with the presence of higher biomass concentrations. [ABSTRACT FROM AUTHOR]

• Hyperfine sub-boundaries (sub-Bs) are found in the grain-boundary free Si thin films. • The grain-boundary free film was obtained by the continuous-wave laser crystallization. • Hyperfine sub-Bs are aligned to the laser scan direction at a ∼3.1 μm period. • The rotation angle of the hyperfine sub-B was 0.061 degrees. Periodic dimple lines have been found by the atomic-force-microscopy on the surface of the grain-boundary free (100) Si films obtained by the continuous-wave laser crystallization. The dimple lines are straight and run parallel each other to the scan direction regularly at a period of ∼3.1 μm. The depth of the dimple lines is 3∼5 nm (peak-to-valley). The internal angle of the dimple lines is ∼179.4°. These dimple lines originate from hyperfine sub-boundaries with a boundary energy of 2.4 × 10−6 J/cm2, a rotation angle θ of ∼0.061°, and a dislocation spacing of ∼0.36 μm. [ABSTRACT FROM AUTHOR]

Increased computing power has made it possible to run simulations of the Weather Research and Forecasting (WRF) numerical model in high spatial resolution. However, running high-resolution simulations requires a higher-detail mapping of landforms, land use, and land cover. Often, higher-resolution data have limited coverage or availability. This paper presents the feasibility of using CORINE Land Cover (CLC) land use and land cover data and alternative high-resolution global coverage land use/land cover (LULC) data from Copernicus Global Land Service Land Cover Map (CGLS-LC100) V2.0 in high-resolution WRF simulations (100 × 100 m). Global LULC data with a resolution of 100 m are particularly relevant for areas not covered by CLC. This paper presents the method developed by the authors for reclassifying land cover data from CGLS-LC100 to MODIS land use classes with defined parameters in the WRF model and describes the procedure for their implementation into the model. The obtained simulation results of the basic meteorological parameters from the WRF simulation using CLC, CGLS-LC100 and default geographical data from MODIS were compared to observations from 13 meteorological stations in the Warsaw area. The research has indicated noticeable changes in the forecasts of temperature, relative humidity wind speed, and direction after using higher-resolution LULC data. The verification results show a significant difference in weather predictions in terms of CLC and CGLS-LC100 LULC data implementation. Due to the fact that better results were obtained for CLC simulations than for CGLS-LC100, it is suggested that CLC data are first used for simulations in numerical weather prediction models and to use CGLS-LC100 data when the area is outside of CLC coverage.

Chemical looping combustion is one novel technology for controlling CO 2 emission with a low energy cost. Due to a lack of understanding of the detailed and micro behavior of the CLC process, especially for a three dimensional structure, numerical simulations are carried out in this work. The configuration is built according to the experimental unit and gaseous fuel is used in this work. A two-fluid model considering heterogeneous reactions is established, and the flow behaviour and reaction characteristics are obtained. The temperature in the air reactor increases with height owing to the exothermic reaction of the xidation of the oxygen carrier, while the temperature in the fuel reactor decreases with height due to the endothermic reaction. The oxidation level of the oxygen carrier is obtained by simulation, which is hard for measurement, and the difference between the inlet and outlet is 0.065. The influences of the operating temperature and injection rate of fuel are presented to understand the performance of the system. The highest fuel conversion rate reaches 0.92 under high operating temperature. The numerical results are helpful for acquiring insight on the flow and reactive behaviour of CLC reactors. [ABSTRACT FROM AUTHOR]

In plants, chloride channels (CLC) are involved in a series of specific functions, such as regulation of nutrient transport and stress tolerance. Members of the wheat Triticum aestivum L. CLC (TaCLC) gene family have been proposed to encode anion channels/transporters that may be related to nitrogen transportation. To better understand their roles, TaCLC family was screened and 23 TaCLC gene sequences were identified using a Hidden Markov Model in conjunction with wheat genome database. Gene structure, chromosome location, conserved motif, and expression pattern of the resulting family members were then analyzed. Phylogenetic analysis showed that the TaCLC family can be divided into two subclasses (I and II) and seven clusters (-a, -c1, -c2, -e, -f1, -f2, and -g2). Using a wheat RNA-seq database, the expression pattern of TaCLC family members was determined to be an inducible expression type. In addition, seven genes from seven different clusters were selected for quantitative real-time PCR (qRT-PCR) analysis under low nitrogen stress or salt stress conditions, respectively. The results indicated that the gene expression levels of this family were up-regulated under low nitrogen stress and salt stress, except the genes of TaCLC-c2 cluster which were from subfamily -c. The yeast complementary experiments illustrated that TaCLC-a-6AS-1 , TaCLC-c1-3AS , and TaCLC-e-3AL all had anion transport functions for NO3− or Cl−, and compensated the hypersensitivity of yeast GEF1 mutant strain YJR040w (Δgef1) in restoring anion-sensitive phenotype. This study establishes a theoretical foundation for further functional characterization of TaCLC genes and provides an initial reference for better understanding nitrate nitrogen transportation in wheat. [ABSTRACT FROM AUTHOR]

Chloride channel protein (ClC) is an extremely widely distributed anion channel that exists on the cell membrane of organisms. It plays a very important function in various physiological processes such as salt stress resistance. Using bioinformatics methods, 10 SbClCs genes were identified in the sorghum genome, and the SbClCs gene family was analyzed by genome identification, promoters, conservative motifs, phylogenetic trees and gene expression. The results showed that the SbClCs gene family is mainly distributed on six chromosomes, SBI-01, SBI-03, SBI-04, SBI-06, SBI-07 and SBI-10; SbClCs proteins are all hydrophobins and have good fluidity. The secondary structure is mainly random coils and α-helix; the analysis results of gene protein domains showed that the relatively conservative domains are Voltage gated and CBS, and ClC family members are relatively conservative in protein. After analyzing the cis-acting elements of SbClCs gene promoters, it was found that many lightresponsive elements (Sp1, G-box) existed. Except for the SbClCg1 gene, the promoter regions of other genes all have droughtinduced-related MYB binding sites (MBS); system phylogenetic tree analysis showed that sorghum ClC is more closely related to corn and rice; gene expression heat map analysis showed that SbClCc3 and SbClCa genes play an important role in salt stress and stress tolerance of sorghum. Selection evolution analysis showed that ClC protein coding genes are also subject to purification selection. This study preliminarily analyzed the structure and expression of 10 chloride ion transporter genes in sorghum, which provided a basis for further research on genetic transformation and molecular breeding with SbClCs. [ABSTRACT FROM AUTHOR]

Glycerol is widely used as a cryoprotectant to protect the sperm from freezing damage during cryopreservation. However, glycerol at a high concentration has toxic effects on the sperm. Therefore, we explored the effects of partially replacing glycerol with cholesterol-loaded cyclodextrin (CLC) in a cryoprotectant on protamine deficiency, in vitro capacitation, and fertilization ability of freeze–thawed Yanbian Yellow cattle sperm. We used fresh semen, control (6% glycerol), and four treatment—I, II, III, and IV (3% glycerol + 0, 0.75, 1.5, and 3 mg/mL CLC, respectively)—groups. Computer-assisted semen analysis; JC-1, CMA3, and FluoZin-3-AM staining; flow cytometry; and IVF were conducted. Replacing a portion of glycerol with 1.5 mg/mL CLC significantly improved sperm motility, viability, plasma membrane integrity, acrosome integrity, and membrane lipid disorders, mitochondrial membrane potential (MMP), capacitation, and fertilization ability (P < 0.05) compared with the control. Additionally, in group I and III, the protamine deficiency were significantly lower (P < 0.05) than in the control group. It was found that 6% glycerol has a higher degree of damage to sperm DNA integrity than 3% glycerol. Overall, this study revealed that partial replacement of glycerol with CLC can be used as a novel cryoprotection method to reduce the toxicity of glycerol and improve the quality of thawed Yanbian Yellow cattle sperm. • CLC (1.5 mg/mL) and 3% glycerol improved sperm development ability in vitro compared to 6% glycerol. • Substitution of CLC for glycerol improved thawed Yanbian yellow cattle sperm quality. • Decreasing the concentration of glycerol and adding CLC can reduce the damage to sperm DNA. • Improving Yanbian yellow cattle sperm preservation will be helpful to breeders. [ABSTRACT FROM AUTHOR]

Deforestation could influence land cover ecological significance in hunting grounds on a big scale. This research examines changes of forest and shrub patches in the Vojvodina region by a set of landscape metrics. The study area includes 154 hunting grounds from which hunting organizations are managing with 134 hunting grounds, PC "Vojvodinasume" with 17, Serbian Armed Forces with two, and National Park "Fruska gora" with one hunting ground. The results show that in the National Park and Serbian Armed Forces hunting grounds the broad-leaved forest areas occurred on the surfaces that were under the transitional woodland-shrub and vice versa. A similar situation is in the hunting grounds managed by PC ''Vojvodinasume'' where there is not only the most intensive logging of broad-leaved and coniferous forests but also the most intensive afforestation of mixed forests and transitional woodland-shrub. On the other hand, in the hunting grounds managed by hunting organizations, there is the highest area growth under the broad-leaved forest.

In plants, chloride channels (CLC) are involved in a series of specific functions, such as regulation of nutrient transport and stress tolerance. Members of the wheat Triticum aestivum L. CLC (TaCLC) gene family have been proposed to encode anion channels/transporters that may be related to nitrogen transportation. To better understand their roles, TaCLC family was screened and 23 TaCLC gene sequences were identified using a Hidden Markov Model in conjunction with wheat genome database. Gene structure, chromosome location, conserved motif, and expression pattern of the resulting family members were then analyzed. Phylogenetic analysis showed that the TaCLC family can be divided into two subclasses (I and II) and seven clusters (-a, -c1, -c2, -e, -f1, -f2, and -g2). Using a wheat RNA-seq database, the expression pattern of TaCLC family members was determined to be an inducible expression type. In addition, seven genes from seven different clusters were selected for quantitative real-time PCR (qRT-PCR) analysis under low nitrogen stress or salt stress conditions, respectively. The results indicated that the gene expression levels of this family were up-regulated under low nitrogen stress and salt stress, except the genes of TaCLC-c2 cluster which were from subfamily -c. The yeast complementary experiments illustrated that TaCLC-a-6AS-1, TaCLC-c1-3AS, and TaCLC-e-3AL all had anion transport functions for NO3− or Cl−, and compensated the hypersensitivity of yeast GEF1 mutant strain YJR040w (Δgef1) in restoring anion-sensitive phenotype. This study establishes a theoretical foundation for further functional characterization of TaCLC genes and provides an initial reference for better understanding nitrate nitrogen transportation in wheat.

The trans-Golgi network/early endosome (TGN/EE) serves as the central hub in which exocytic and endocytic trafficking pathways converge and specificity of cargo routing needs to be achieved. Acidification is a hallmark of the TGN/EE and is maintained by the vacuolar H+-ATPase (V-ATPase) with support of proton-coupled antiporters. We show here that ClCd and ClCf, two distantly related members of the Arabidopsis Cl- channel (ClC) family, colocalize in the TGN/EE, where they act redundantly, and are essential for male gametophyte development. Combining an inducible knockdown approach and in vivo pH measurements, we show here that reduced ClC activity does not affect pH in the TGN/EE but causes hyperacidification of trans-Golgi cisternae. Taken together, our results show that ClC-mediated anion transport into the TGN/EE is essential and affects spatiotemporal aspects of TGN/EE maturation as well as its functional separation from the Golgi stack. [ABSTRACT FROM AUTHOR]

Oil pollution damage caused by oil spills at sea generally occurs on a large scale across numerous regions and countries, causing significant harm to marine ecosystems as well as worldwide economic loss. The costs are so severe in many instances that the owner of the ship responsible for the pollution cannot afford to pay compensation to those who have suffered loss. As a consequence, the need to cover oil pollution damages has given rise to compulsory liability insurance, which provides a financial guarantee against the costs of oil spills. Compulsory civil liability insurance has therefore become an indispensable part of the liability regime for owners of oil tankers and bunkers. [ABSTRACT FROM AUTHOR]

As the world's population grows, so does the pressure on agricultural land. Consequently, there is less and less agricultural land. Bosnia and Herzegovina is one of the countries whose population is decreasing from year to year, partly owing to negative natural increase and partly owing to the migrations of young people to developed western countries. However, despite the depopulation trend, there has been a significant decline in agricultural land in the first two decades of the twenty-first century. The reasons should be sought in various socio-economic processes that crucially affect spatial development. In order to understand the changes that are happening in the field, the paper analyzes the structure of agricultural land losses in B&H based on the database of the European project CORINE Land Cover. In relation to the five main land cover categories (artificial surfaces, agricultural areas, forests and semi-natural areas, wetlands and water bodies), the data show that in the period from 2000 to 2018 artificial areas increased spatial coverage, forests and semi-natural areas as well, while agricultural land decreased. The aim of the research is to identify the spatially largest changes of agricultural land subclasses into other types of land cover, in order to determine the causes and areas with the most intense pressure. This is essential given the fact that agricultural land is becoming an increasingly important natural resource over time. Based on the recent demographic and socio-economic trends, it is to be expected that the trend of decreasing agricultural land will continue in the future, but with a reduced intensity. [ABSTRACT FROM AUTHOR]

Microorganisms contend with numerous and unusual chemical threats and have evolved a catalog of resistance mechanisms in response. One particularly ancient, pernicious threat is posed by fluoride ion (F−), a common xenobiotic in natural environments that causes broad-spectrum harm to metabolic pathways. This review focuses on advances in the last ten years toward understanding the microbial response to cytoplasmic accumulation of F−, with a special emphasis on the structure and mechanisms of the proteins that microbes use to export fluoride: the CLCF family of F−/H+ antiporters and the Fluc/FEX family of F− channels. [ABSTRACT FROM AUTHOR]

Microorganisms resist fluoride toxicity using fluoride export proteins from one of several different molecular families. Cariogenic species Streptococcus mutans and Candida albicans extrude intracellular fluoride using a CLC F F - /H + antiporter and FEX fluoride channel, respectively, whereas oral commensal eubacteria, such as Streptococcus gordonii, export fluoride using a Fluc fluoride channel. In this work, we examine how genetic knockout of fluoride export impacts pathogen fitness in single-species and three-species dental biofilm models. For biofilms generated using S. mutans with the genetic knockout of the CLC F transporter, exposure to low fluoride concentrations decreased S. mutans counts, synergistically reduced the populations of C. albicans , increased the relative proportion of oral commensal S. gordonii , and reduced properties associated with biofilm pathogenicity, including acid production and hydroxyapatite dissolution. Biofilms prepared with C. albicans with genetic knockout of the FEX channel also exhibited reduced fitness in the presence of fluoride but to a lesser degree. Imaging studies indicate that S. mutans is highly sensitive to fluoride, with the knockout strain undergoing complete lysis when exposed to low fluoride for a moderate amount of time. Biochemical purification of the S. mutans CLC F transporter and functional reconstitution establishes that the functional protein is a dimer encoded by a single gene. Together, these findings suggest that fluoride export by oral pathogens can be targeted by specific inhibitors to restore biofilm symbiosis in dental biofilms and that S. mutans is especially susceptible to fluoride toxicity., Importance: Dental caries is a globally prevalent condition that occurs when pathogenic species, including Streptococcus mutans and Candida albicans , outcompete beneficial species, such as Streptococcus gordonii , in the dental biofilm. Fluoride is routinely used in oral hygiene to prevent dental caries. Fluoride also has antimicrobial properties, although most microbes possess fluoride exporters to resist its toxicity. This work shows that sensitization of cariogenic species S. mutans and C. albicans to fluoride by genetic knockout of fluoride exporters alters the microbial composition and pathogenic properties of dental biofilms. These results suggest that the development of drugs that inhibit fluoride exporters could potentiate the anticaries effect of fluoride in over-the-counter products like toothpaste and mouth rinses. This is a novel strategy to treat dental caries., Competing Interests: The authors declare no conflict of interest.

Deforestation could influence land cover ecological significance in hunting grounds on a big scale. This research examines changes of forest and shrub patches in the Vojvodina region by a set of landscape metrics. The study area includes 154 hunting grounds from which hunting organizations are managing with 134 hunting grounds, PC "Vojvodinasume" with 17, Serbian Armed Forces with two, and National Park "Fruska gora" with one hunting ground. The results show that in the National Park and Serbian Armed Forces hunting grounds the broad-leaved forest areas occurred on the surfaces that were under the transitional woodland-shrub and vice versa. A similar situation is in the hunting grounds managed by PC Vojvodinasume where there is not only the most intensive logging of broad-leaved and coniferous forests but also the most intensive afforestation of mixed forests and transitional woodland-shrub. On the other hand, in the hunting grounds managed by hunting organizations, there is the highest area growth under the broad-leaved forest.