84 results on '"CIPRIANI, B."'
Search Results
2. Le Regole per ben sonare il cembalo di Alessandro Scarlatti
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Cipriani, B
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Settore L-ART/07 - Published
- 2021
3. La tradizione del partimento a Roma e le «Regole per ben [son]are il cembalo [...]» di Alessandro Scarlatti
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Cipriani, B
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Settore L-ART/07 ,Partimento ,Scarlatti - Published
- 2020
4. Adenovirus as vehicle for anticancer genetic immunotherapy
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Gallo, P, Dharmapuri, S, Cipriani, B, and Monaci, P
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- 2005
- Full Text
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5. P1552Different forms of evaluation of atherosclerotic load and its different predicted powers compared with framingham risk score
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Schiavone, M, primary, Gonzalez, S A, additional, Cipriani, B, additional, Melchiori, R, additional, Kempny, P, additional, Chiabaut-Svane, J, additional, Brenzoni, N, additional, Ferroni, F, additional, Filosa, E, additional, Ayerdi, L, additional, Failo, M, additional, and Castellaro, C, additional
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- 2019
- Full Text
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6. La didattica musicale del partimento: la situazione del contesto romano tra figure chiave e nuove fonti
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Cipriani, B
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Partimento ,Settore L-ART/07 - Musicologia e Storia della Musica - Published
- 2019
7. I partimenti di Raffaele Caravaglios. Fondo Raffaele Caravaglios I-Rbc Ms. C 102
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Cipriani, B
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Caravaglios ,Partimento ,Settore L-ART/07 - Musicologia e Storia della Musica - Published
- 2018
8. FeGa/MgO/Fe/GaAs(001) magnetic tunnel junction: Growth and magnetic properties
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B. Gobaut (a), R. Cipriani (b), B.R. Salles (b), D. Krizmancic (b), G. Rossi (b, G. Panaccione (b), M. Eddrief (c, M. Marangolo (c, P. Torelli(b), Institut des Nanosciences de Paris (INSP), and Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)
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Magneto strictive ,Magnetostrictive ,Galfenol ,Magnetic tunnel junction ,XMCD ,Materials science ,Condensed matter physics ,Spintronics ,Magnetostrictive, Galfenol, Magnetic tunnel junction, XMCD ,Magnetostriction ,Condensed Matter::Mesoscopic Systems and Quantum Hall Effect ,Condensed Matter Physics ,Electronic, Optical and Magnetic Materials ,Condensed Matter::Materials Science ,Tunnel magnetoresistance ,Magnetic anisotropy ,Hysteresis ,Ferromagnetism ,Magneto-optic Kerr effect ,Condensed Matter::Superconductivity ,Multiferroics ,[PHYS.COND]Physics [physics]/Condensed Matter [cond-mat] - Abstract
International audience; Research on spintronics and on multiferroics leads now to the possibility of combining the properties of these materials in order to develop new functional devices. Here we report the integration of a layer of magnetostrictive material into a magnetic tunnel junction. A FeGa/MgO/Fe heterostructure has been grown on a GaAs(001) substrate by molecular beam epitaxy (MBE) and studied by X-ray magnetic circular dichroism (XMCD). The comparison between magneto optical Kerr effect (MOKE) measurements and hysteresis performed in total electron yield allowed distinguishing the ferromagnetic hysteresis loop of the FeGa top layer from that of the Fe buried layer, evidencing a different switching field of the two layers. This observation indicates an absence of magnetic coupling between the two ferromagnetic layers despite the thickness of the MgO barrier of only 2.5 nm. The in plane magnetic anisotropy has also been investigated. Overall results show the good quality of the heterostructure and the general feasibility of such a device using magnetostrictive materials in magnetic tunnel junction. (C) 2014 Elsevier B.V. All rights reserved
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- 2015
9. Evidence for a role of γδ T cells in demyelinating diseases as determined by activation states and responses to lipid antigens
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Borsellino, G, Koul, O, Placido, R, Tramonti, D, Luchetti, S, Galgani, S, Salvetti, M, Gasperini, C, Ristori, G, Bonetti, B, Bach, S, Cipriani, B, and Battistini, L
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- 2000
- Full Text
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10. Antigen presenting capacity of brain microvasculature in altered peptide ligand modulation of experimental allergic encephalomyelitis
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Santambrogio, L, Pakaski, M, Wong, M.L, Cipriani, B, Brosnan, C.F, Lees, M.B, and Dorf, M.E
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- 1999
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11. Il Concerto Musicale di Gavignano e la Canzone alla Vergine delle Grazie
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Cipriani, B
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Settore L-ART/07 - Musicologia e Storia della Musica - Published
- 2015
12. Tradition and modernity in the mediterranean cities. The tunisian cities: Tunis, Kairouan, Tozeur, Nefta
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MICARA, L, PETRUCCIOLI, A, VADINI, E, RAYMOND, A, BOUGHERIRA HADJI, Q, BRAMBILLA, G, BSHARA, K, CHENNAOUI, Y, MASSARENTE, A, MILOJEVIC, M, SAIDI, M, LIBRATO, P, ALEMI, M, DE MARCO, A, GUGLIUZZO, C, LANGELLA, C, MININNI, M, POZZI, C, TROVATO, MG, VAN DER MEERSCHEN, M, VENEZIANO, R, VUSKANOVIC, D, RUGGERI, R, ATZENI, C, BALAMIR, A, URAZ, T, DE FILIPPI, F, CALTABIANO, I, DEVIREN, A, DI CRISTINA, B, EL HARROUNI, K, GITTO, E, LEPORE, M, MOCCIA, C, NEGLIA, A, NTEFEH, R, MARENNE, C, SIRET, D, QASEM, M, RAFFA, P, SIBLEY, M, ABDELSALAM, T, BOUTEFLIKA, M, DINCYUREK, O, TURKER, O, AMBROGIO, A, CAROCCI, C, CERADINI, V, DE CUNZO, M, TERENZI, P, ANDRIANI, G, CATANIA, D, GUASTAMACCHIA, L, ANSARI, Z, BARTOLI, B, BIONDI, B, CIRINNA, C, MECCA, S, SACCHETTI, F, BRADASCHIA, M, BUGATTI, E, CADONI, G, MOSETTO, A, CARLOTTI, P, CERASI, M, CHABBI CHEMROUK, N, LAHLOUH, G, KHALIL, F, CHIAS, P, CIPRIANI, B, CIRANNA, S, CRITES, D, CROCKER, C, DAOUDI, NS, BENSALEM, R, DE GIOVANNI, G, DE MIRANDA, A, EISSA, MA, ESCHER, A, EYUPGILLER, K, TOPCUBASI, M, FENTRESS, E, FORLANI, MC, GENOVESI, E, GIRGENTI, GM, AL HAGLA, K, HOHMANN VOGRIN, A, KAHLAOUI, T, KAROUI, H, KHALILL, F, LAMBERTI, A, LAMEI, S, MONTALBANO, C, PINTO, G, PISANO, L, RUBINI, C, TRAVERSA, P, PROCIDA, E, EL RAFEY, M, RAMOS, AM, RIZZI, N, AVARELLO, P, PANIZZA, M, CANCIANI, M, FIDANZA, A, OMBUEN, S, RUGGERI, A, SAAD, PC, SAGDIC, Z, TAHARI, H, TOSUN, CB, TOXEY, AP, ZERARKA, M., GAROFALO, V, SARRO, Adriana, MICARA, L., PETRUCCIOLI, A., VADINI, E., MICARA, L, PETRUCCIOLI, A, VADINI, E, RAYMOND, A, BOUGHERIRA-HADJI, Q, BRAMBILLA, G, BSHARA, K, CHENNAOUI, Y, MASSARENTE, A, MILOJEVIC, M, SAIDI, M, LIBRATO, P, ALEMI, M, DE MARCO, A, GUGLIUZZO, C, LANGELLA, C, MININNI, M, POZZI, C, TROVATO, MG, VAN DER MEERSCHEN, M, VENEZIANO, R, VUSKANOVIC, D, RUGGERI, R, ATZENI, C, BALAMIR, A, URAZ, T, DE FILIPPI, F, CALTABIANO, I, DEVIREN, A, DI CRISTINA, B, EL HARROUNI, K, GITTO, E, LEPORE, M, MOCCIA, C, NEGLIA, A, NTEFEH, R, MARENNE, C, SIRET, D, QASEM, M, RAFFA, P, SIBLEY, M, ABDELSALAM, T, BOUTEFLIKA, M, DINCYUREK, O, TURKER, O, AMBROGIO, A, CAROCCI, C, CERADINI, V, DE CUNZO, M, TERENZI, P, ANDRIANI, G, CATANIA, D, GUASTAMACCHIA, L, ANSARI, Z, BARTOLI, B, BIONDI, B, CIRINNA, C, MECCA, S, SACCHETTI, F, BRADASCHIA, M, BUGATTI, E, CADONI, G, MOSETTO, A, CARLOTTI, P, CERASI, M, CHABBI-CHEMROUK, N, LAHLOUH, G, KHALIL, F, CHIAS, P, CIPRIANI, B, CIRANNA, S, CRITES, D, CROCKER, C, DAOUDI, NS, BENSALEM, R, DE GIOVANNI, G, DE MIRANDA, A, EISSA, MA, ESCHER, A, EYUPGILLER, K, TOPCUBASI, M, FENTRESS, E, FORLANI, MC, GAROFALO, V, GENOVESI, E, GIRGENTI, GM, AL-HAGLA, K, HOHMANN-VOGRIN, A, KAHLAOUI, T, KAROUI, H, KHALILL, F, LAMBERTI, A, LAMEI, S, MONTALBANO, C, PINTO, G, PISANO, L, RUBINI, C, TRAVERSA, P, PROCIDA, E, EL-RAFEY, M, RAMOS, AM, RIZZI, N, AVARELLO, P, PANIZZA, M, CANCIANI, M, FIDANZA, A, OMBUEN, S, RUGGERI, A, SAAD, PC, SAGDIC, Z, SARRO, A, TAHARI, H, TOSUN, CB, TOXEY, AP, and ZERARKA, M
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tozeur ,mediterraneo ,tunisi ,Settore ICAR/14 - Composizione Architettonica E Urbana ,nefta ,mediterranean ,kairouan - Abstract
L’articolo fa un confronto tra le differenti forme urbane delle città del Nord Africa, in particolare quelle della Tunisia (Tunisi, Kairouan, Tozeur, Nefta). Tali città, distribuite tra il Nord ed il Sud del paese, esprimono, nelle loro medine, l’identità della forma urbana e ne dichiarano la modernità lungo i loro bordi. Tunisi, posta tra due laghi, costruisce la sua modernità con la città coloniale che, anche se con linguaggi diversi, evidenzia i temi del Mediterraneo. La città di Kairouan mantiene la sua identità nella grande moschea, luogo centrale di organizzazione urbana. Le città d’oasi, come Tozeur e Nefta, esprimono il loro rapporto col paesaggio ed evidenziano con le loro architetture i materiali usati.
- Published
- 2009
13. Small bowel intussussception due to metastatic melanoma of unknown primary site. Case report
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STAGNITTI, F., ORSINI, S., MARTELLUCCI, A., TUDISCO, A., AVALLONE, M., AIUTI, F., DI GIROLAMO, V., STEFANELLI, F., DE ANGELIS, F., COSTANTINO, A., DI GRAZIA, C., NAPOLEONI, A., NICODEMI, S., CIPRIANI, B., CECI, F., MOSILLO, R., CORELLI, S., CASCIARO, G., and SPAZIANI, E.
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Male ,Oncology ,Pathology ,medicine.medical_specialty ,Ileum ,Metastasis ,Clinical Practice ,Internal medicine ,medicine ,Humans ,Malignant melanoma ,Unknown primary ,Intestinal intussusceptions ,Disseminated disease ,Melanoma ,Gastrointestinal tract ,business.industry ,Middle Aged ,medicine.disease ,Ileal Neoplasms ,medicine.anatomical_structure ,Iron-deficiency anemia ,Neoplasms, Unknown Primary ,Segmental resection ,business ,Intestinal Obstruction ,Brain metastasis - Abstract
Malignant melanoma is characterized by metastases also to the gastrointestinal tract, especially in the small bowel. The diagnosis is often delayed because unspecific clinical presentation (frequently as chronic iron deficiency anemia, rectal bleeding or intestinal obstruction). We present a case of melanoma of unknown primary site, with clinical presentation of intestinal obstruction. A segmental resection of the ileum was performed including mesentery with lymph nodes. Histology revealed metastatic melanoma from unknown primary. PET and MRI confirmed disseminated disease without brain metastasis.
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- 2014
14. Risk management in surgery
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Messano, GIUSEPPE ALESSIO, Spaziani, Erasmo, Turchetta, F., Ceci, F., Corelli, S., Casciaro, Giovanni Enrico, Martellucci, A., Costantino, A., Napoleoni, A., Cipriani, B., Nicodemi, S., Di Grazia, C., Mosillo, R., Avallone, M., Orsini, S., Tudisco, A., Aiuti, F., and Stagnitti, Franco
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antibiotic therapy ,laparoscopic drainage ,liver abscesses ,percutaneous drainage ,risk management ,Risk Management ,Italy ,Surgical Procedures, Operative ,Humans ,Focus on ,Checklist - Abstract
Malpractice is the responsible for the greatest number of legal claims. At the present time, legal actions against physicians in Italy are 15,000 per year, and a stunning increase about costs to refund patients injured by therapeutic and diagnostic errors is expected. The method for the medical prevention is “Risk Management”, that is the setting-up of organizational instruments, methods and actions that enable the measurement or estimation of medical risk; it allows to develop strategies to govern and reduce medical error. In the present work, the reconstruction about the history of risk management in Italy was carried out. After then the latest initiatives undertaken by Italy about the issue of risk management were examined.
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- 2013
15. [Unusual cause of acute abdomen in adult patient: gallstone ileus]
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Spaziani, Erasmo, Vergaro, C, Picchio, M, DI FILIPPO, Annalisa Romina, Ceci, F, Faiola, E, Ottaviani, M, Parisella, F, Martellucci, A, Stefanelli, F, Nardecchia, G, Iorio, O, DE ANGELIS, F, Cipriani, B, Nicodemi, S, Pattaro, G, and Stagnitti, Franco
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Abdomen, Acute ,Diagnosis, Differential ,Male ,Ileus ,Treatment Outcome ,Ileal Diseases ,Intestinal Fistula ,Humans ,Cholecystectomy ,Gallstones ,Middle Aged ,Tomography, X-Ray Computed - Abstract
Gallstone ileus is a rare complication of cholecystolithiasis. It causes 1-3% of the mechanical obstructions of the small bowel. It often affects patients between 63 and 85 years old. Pre-operative diagnosis is usually delayed 1-10 days because there is not a specific symptomatology.The authors report the case of a 50 year-old man with diagnosis of mechanical obstruction of the small bowel caused by a voluminous gallstone. Ileal occlusion was showed by CT. The patient underwent to one-stage emergency surgery with enterolithotomy, cholecystectomy and duodenal fistula repair. Patient's recovery was regular and he was discharged fourteen days after surgery.In our case gallstone ileus was diagnosed with a delay of 5 days. Ultrasonography was not able to show the gallbladder. Diagnosis was made by CT, which is the diagnostic gold standard.Patient's performance status influences surgical strategy. In our experience, the patient underwent one-stage surgery because he was considered at low risk. Instead staged procedure with enterolithotomy and delayed cholecystectomy and fistula repair, is performed only in patients at high risk.
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- 2010
16. A case of uterine carcinosarcoma
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Spaziani E, Picchio M, Di Filippo A, Vincenzo Petrozza, Censi F, Sardella B, Briganti M, Ceci F, Nicodemi S, Nardecchia G, De Angelis F, Cipriani B, and Stagnitti F
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Aged, 80 and over ,Carcinosarcoma ,Uterine Neoplasms ,Humans ,Female - Abstract
Uterine carcinosarcoma is a highly aggressive neoplasm with tendency to early recurrence and/or metastasis. The neoplasia has both epithelial and stromal malignant components. Clinically, it is characterized by a postmenopausal metrorrhagia, associated sometimes with abdominal pain. The Authors describe a case of carcinosarcoma in a 82-year old woman with a vaginal bleeding since 10 months and an ingravescent hypochromic anemia. The RMN of the abdomen and pelvis showed a mass occupying the whole uterine lumen, without signs of pelvic diffusion. A bilateral hystero-oophorectomy was performed; the patient's critical conditions and a high anaesthesiological risk advised us not to perform a bilateral pelvic lymphadenectomy with aortic sampling. Six months after operation, a clinical follow-up with a total-body CT didn't show any sign of local recurrence and/or distant metastasis. From the analysis of this case report and from the data of the literature some important considerations can be done: a) gynecologic check up in post-menopausal women with associated risk factors (obesity and hypertension) can allow an early diagnosis also in asymptomatic patients; b) sovrapubic and transvaginal US, abdomino-pelvic CT and MRI are essential for a correct clinical staging; c) surgical excision followed by the histological examination of the specimen is mandatory to establish a correct diagnosis.
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- 2008
17. Adenovirus vaccination against neu oncogene exerts long-term protection from tumorigenesis in BALB/neuT transgenic mice
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Gallo, P, Dharmapuri, S, Nuzzo, M, Maldini, D, Cipriani, B, Forni, Guido, and Monaci, P.
- Published
- 2007
18. Phenotypic and functional properties of gd T cells from patients with Guillain-Barré syndrome
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Borsellino, G., Poccia, F., Placido, R., Tramonti, D., Mancino, G., Luchetti, S., Galgani, S., Bonetti, Bruno, Bach, S., Cipriani, B., Brosnan, C. F., and Battistini, L.
- Published
- 2000
19. Biliodigestive fistulae and gallstone ileus: diagnostic and therapeutic considerations. Our experience.
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STAGNITTI, F., TUDISCO, A., CECI, F., NICODEMI, S., ORSINI, S., AVALLONE, M., DI GIROLAMO, V., STEFANELLI, F., DE ANGELIS, F., DI GRAZIA, C., CIPRIANI, B., AIUTI, F., NAPOLEONI, A., MOSILLO, R., CORELLI, S., CASCIARO, G., COSTANTINO, A., MARTELLUCCI, A., and SPAZIANI, E.
- Published
- 2014
20. CD94/NKG2 inhibitory receptor complex modulates both anti-viral and anti-tumoral responses of polyclonal phosphoantigen-reactive V gamma 9V delta 2 T lymphocytes.
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Poccia, F, primary, Cipriani, B, additional, Vendetti, S, additional, Colizzi, V, additional, Poquet, Y, additional, Battistini, L, additional, López-Botet, M, additional, Fournié, J J, additional, and Gougeon, M L, additional
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- 1997
- Full Text
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21. Single-incision laparoscopic appendectomy is comparable to conventional laparoscopic and laparotomic appendectomy: our single center single surgeon experience.
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CECI, F., ORSINI, S., TUDISCO, A., AVALLONE, M., AIUTI, F., GIROLAMO, V. DI, STEFANELLI, F., DE ANGELIS, F., MARTELLUCCI, A., COSTANTINO, A., DI GRAZIA, C., NICODEMI, S., CIPRIANI, B., NAPOLEONI, A., MOSILLO, R., CORELLI, S., CASCIARO, G., SPAZIANI, E., and STAGNITTI, F.
- Published
- 2013
22. Technique and outcomes about a new laparoscopic procedure: the Pelvic Organ Prolapse Suspension (POPS).
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CECI, F., SPAZIANI, E., CORELLI, S., CASCIARO, G., MARTELLUCCI, A., COSTANTINO, A., NAPOLEONI, A., CIPRIANI, B., NICODEMI, S., DI GRAZIA, C., AVALLONE, M., ORSINI, S., TUDISCO, A., AIUTI, F., and STAGNITTI, F.
- Published
- 2013
23. Cholecystectomy by single incision laparoscopic surgery (SILS): early experience and technique standardization.
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Ceci, F., di Grazia, C., Cipriani, B., Nicodemi, S., Corelli, S., Pecchia, M., Martellucci, A., Costantino, A., Stefanelli, F., Salvadori, C., Napoleoni, A., Parisella, M., Spaziani, E., and Stagnitti, F.
- Published
- 2012
24. Liver resection for hemoperitoneum caused by spontaneous rupture of unrecognized hepatocellular carcinoma.
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Casciaro, G. E., Spaziani, E., Costantino, A., Ceci, F., Di Grezia, C., Martellucci, A., Pecchia, M., Cipriani, B., De Angelis, F., Corelli, S., Napoleoni, A., Stefanelli, F., Salvadori, C., Parisella, M., Nicodemi, S., and Stagnitti, F.
- Published
- 2012
25. Insolita causa di addome acuto in paziente adulto: l'ileo biliare.
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Spaziani, E., Vergaro, C., Picchio, M., Di Filippo, A., Ceci, F., Faiola, E., Ottaviani, M., Parisella, F., Martellucci, A., Stefanelli, F., Nardecchia, G., Iorio, O., De Angelis, F., Cipriani, B., Nicodemi, S., Pattaro, G., and Stagnitti, F.
- Published
- 2010
26. Phosphoantigen-reactive Vgamma9Vdelta2 T lymphocytes suppress in vitro human immunodeficiency virus type 1 replication by cell-released antiviral factors including CC chemokines.
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Poccia, Fabrizio, Battistini, Luca, Cipriani, Barbara, Mancino, Giorgio, Martini, Federico, Gougeon, Marie Lise, Colizzi, Vittorio, Poccia, F, Battistini, L, Cipriani, B, Mancino, G, Martini, F, Gougeon, M L, and Colizzi, V
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LYMPHOCYTES ,HIV ,CHEMOKINES ,PROTEIN analysis ,BACTERIAL antigens ,CELL culture ,CELL receptors ,COMPARATIVE studies ,CYTOKINES ,ENZYME-linked immunosorbent assay ,FLOW cytometry ,HYDROCARBONS ,IMMUNOLOGY technique ,RESEARCH methodology ,MEDICAL cooperation ,MYCOBACTERIUM ,ORGANOPHOSPHORUS compounds ,PHOSPHOPROTEINS ,PROTEINS ,RESEARCH ,T cells ,EVALUATION research ,PHYSIOLOGY - Abstract
Vgamma9Vdelta2 T lymphocytes are broadly reactive against various intracellular pathogens and display both lytic and proliferative responses to human immunodeficiency virus (HIV)-infected cells. HIV infection of peripheral blood mononuclear cell cultures led to absolute increases in Vgamma9Vdelta2 T cells accompanied by decreased p24 levels. Strong gammadelta T cell activation with nonpeptidic mycobacterial phosphoantigens (TUBAg1 extract or synthetic isopentenyl pyrophosphate) resulted in potent inhibition of HIV replication through soluble released factors. Subsequent analyses showed that phosphoantigen-activated gammadelta T cells produced substantial amounts of beta-chemokines (macrophage inflammatory protein [MIP]-1alpha, MIP-1beta, and regulated-on-activation, normal T-cell-expressed and -secreted beta-chemokine [RANTES]), which represent the natural ligand for the CCR5 HIV coreceptor. Accordingly, anti-beta-chemokine antibodies neutralized the inhibition of monocytotropic HIV strains by gammadelta T cell-released factors. Moreover, a T-tropic HIV strain using the CXCR4 coreceptor for virus entry was potently inhibited. Together, these data reveal that phosphoantigen-activated gammadelta T cells are an important source of CC chemokines and may suppress HIV replication through cell-released antiviral factors. [ABSTRACT FROM AUTHOR]
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- 1999
- Full Text
- View/download PDF
27. Phenotypic and functional properties of @c@d T Cells from patients with Guillain Barre syndrome
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Borsellino, G., Poccia, F., Placido, R., Tramonti, D., Mancino, G., Luchetti, S., Galgani, S., Bonetti, B., Bach, S., and Cipriani, B.
- Published
- 2000
- Full Text
- View/download PDF
28. Cytokine, chemokine and chemokine receptor mRNA expression in different strains of normal mice: implications for establishment of a Th1/Th2 bias
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Charles, P.C., Weber, K.S., Cipriani, B., and Brosnan, C.F.
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- 1999
- Full Text
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29. CD94/NKG2 Inhibitory Receptor Complex Modulates Both Anti-Viral and Anti-Tumoral Responses of Polyclonal Phosphoantigen-Reactive Vγ9Vδ2 T Lymphocytes
- Author
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Poccia, F., Cipriani, B., Silvia Vendetti, Colizzi, V., Poquet, Y., Battistini, L., López-Botet, M., Fournié, J. -J, and Gougeon, M. -L
30. Liver resection for hemoperitoneum caused by spontaneous rupture of unrecognized hepatocellular carcinoma
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giovanni casciaro, Spaziani, E., Costantino, A., Ceci, F., Di Grazia, C., Martellucci, A., Pecchia, M., Cipriani, B., Angelis, F., Corelli, S., Napoleoni, A., Stefanelli, F., Salvadori, C., Parisella, M., Nicodemi, S., and Stagnitti, F.
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Aged, 80 and over ,Male ,Carcinoma, Hepatocellular ,Rupture, Spontaneous ,Hemoperitoneum ,Liver Neoplasms ,Hepatectomy ,Humans - Abstract
Hepatocellular carcinoma (HCC) is an increasingly common form of cancer. Although its spontaneous rupture is rare in Western countries, it constitutes a surgical emergency and is associated with high mortality. There is a lack of consensus as to the best approach and what parameters to use in choosing it. The three main approaches are conservative, endovascular and resection - the treatment of choice for acute abdominal bleeding. We report a case of hemoperitoneum following the spontaneous rupture of an unrecognized HCV-related HCC in a patient with no history of liver disease. The patient was successfully treated by emergency surgery, with resection of two segments of the left liver.
31. Cholecystectomy by single incision laparoscopic surgery (SILS): early experience and technique standardization
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Ceci, F., Carmela Di Grazia, Cipriani, B., Nicodemi, S., Corelli, S., Pecchia, M., Martellucci, A., Costantino, A., Stefanelli, F., Salvadori, C., Napoleoni, A., Parisella, M., Spaziani, E., and Stagnitti, F.
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Adult ,Male ,Young Adult ,Cholecystectomy, Laparoscopic ,Humans ,Female ,Middle Aged ,Aged - Abstract
Single Incision Laparoscopic Surgery (SILS) is a recent surgical technique, first described in the 1990s. Its aim is to optimize the esthetic result offered by laparoscopy by minimizing the number of abdominal incisions. Various preliminary studies have been carried out on the application of SILS, especially in cholecystectomy and appendectomy. This study evaluates the preliminary results of cholecystectomy by SILS (SILS™ Port) conducted between October 2009 and February 2011 on 21 patients (4 men and 17 women) with a mean age of 49.9 years and a mean Body Mass Index (BMI) of 22.8. All patients were treated by the same team, which had previously undergone six months' simulator training. There were two main selection criteria, both evaluated intraoperatively: absence of adhesions and of significant inflammatory sequelae from previous cholecystitis; and suitable distance between gallbladder and SILS access port. Conversion to traditional laparoscopy was necessary in just two cases, while an accessory trocar was introduced in another two cases. Conversion to open surgery was not necessary in any case. One case of SILS cholecystectomy was complicated by postoperative bile leakage, which was treated conservatively, as the fistula had a low output. The mean duration of hospitalization was 3.6 days. This preliminary experience led us to conclude that SILS is safe and highly satisfactory in the postoperative phase, thanks to the reduced need for painkillers and the improved esthetic result.
32. NK1.1+ T cells in experimental autoimmune encephalomyelitis
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Cipriani, B., Rajan, A.J., Brosnan, C.F., and Battistini, L.
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- 1998
- Full Text
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33. Autoimmune B-cell lymphopenia after successful adoptive therapy with telomerase-specific T lymphocytes
- Author
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Carmela Mennuni, Barbara Cipriani, Elisa Peranzoni, Vincenzo Bronte, Francesco Calvaruso, Paola Zanovello, Tania Pannellini, Piero Musiani, Elisa Scarselli, Raffaele De Palma, Stefano Ugel, Lucia Ricci-Vitiani, Manuela Iezzi, Ugel, S, Scarselli, E, Iezzi, M, Mennuni, C, Pannellini, T, Calvaruso, F, Cipriani, B, DE PALMA, Raffaele, RICCI VITIANI, L, Peranzoni, E, Musiani, P, Zanovello, P, and Bronte, V.
- Subjects
Male ,Telomerase ,Adoptive cell transfer ,Lung Neoplasms ,Skin Neoplasms ,medicine.medical_treatment ,T-Lymphocytes ,Inbred C57BL ,Biochemistry ,Transgenic ,Cell therapy ,Mice ,Cancer immunotherapy ,Inside Blood ,Cytotoxic T cell ,Melanoma ,B-Lymphocytes ,Tumor ,Hematology ,Adoptive Transfer ,Colonic Neoplasms ,Cancer Vaccine ,Immunology ,Bone Marrow Cells ,Mice, Transgenic ,Adenocarcinoma ,Cancer Vaccines ,Cancer Immunotherapy ,Animals ,Cell Line ,Disease Models ,Animal ,HLA-A2 Antigen ,Lymphopenia ,Neoplasm Transplantation ,Prostatic Neoplasms ,Spleen ,Cell Line, Tumor ,medicine ,Telomerase reverse transcriptase ,business.industry ,Cancer ,Cell Biology ,Immunotherapy ,medicine.disease ,Mice, Inbred C57BL ,Disease Models, Animal ,business - Abstract
Telomerase reverse transcriptase (TERT) is a good candidate for cancer immunotherapy because it is overexpressed in 85% of all human tumors and implicated in maintenance of the transformed phenotype. TERT-based cancer vaccines have been shown to be safe, not inducing any immune-related pathology, but their impact on tumor progression is modest. Here we show that adoptive cell therapy with the use of high-avidity T lymphocytes reactive against telomerase can control the growth of different established tumors. Moreover, in transgenic adenocarcinoma mouse prostate mice, which develop prostate cancer, TERT-based adoptive cell therapy halted the progression to more aggressive and poorly differentiated tumors, significantly prolonging mouse survival. We also demonstrated that human tumors, including Burkitt lymphoma, and human cancer stem cells, are targeted in vivo by TERT-specific cytotoxic T lymphocytes. Effective therapy with T cells against telomerase, different from active vaccination, however, led to autoimmunity marked by a consistent, although transient, B-cell depletion in primary and secondary lymphoid organs, associated with alteration of the spleen cytoarchitecture. These results indicate B cells as an in vivo target of TERT-specific cytotoxic T lymphocytes during successful immunotherapy.
- Published
- 2009
34. Interaction of Au(III) with amino acids: a vade mecum for medicinal chemistry and nanotechnology.
- Author
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Mattioli EJ, Cipriani B, Zerbetto F, Marforio TD, and Calvaresi M
- Subjects
- Nanotechnology methods, Metal Nanoparticles chemistry, Chemistry, Pharmaceutical, Gold chemistry, Amino Acids chemistry
- Abstract
Au(III) is highly reactive. At odds with its reduced counterpart, Au(I), it is hardly present in structural databases. And yet, it is the starting reactant to form gold nanoclusters (AuNCs) and the constitutive component of a new class of drugs. Its reactivity is a world apart from that of the iso-electronic Pt(II) species. Rather than DNA, it targets proteins. Its interaction with amino acid residues is manifold. It can strongly interact with the residue backbones, amino acid side chains and protein ends, it can form appropriate complexes whose stabilization energy reaches up to more than 40 kcal mol
-1 , it can affect the p Ka of amino acid residues, and it can promote charge transfer from the residues to the amount that it is reduced. Here, quantum chemical calculations provide quantitative information on all the processes where Au(III) can be involved. A myriad of structural arrangements are examined in order to determine the strongest interactions and quantify the amount of charge transfer between protonated and deprotonated residues and Au(III). The calculated interaction energies of the amino acid side chains with Au(III) quantitatively reproduce the experimental tendency of Au(III) to interact with selenocysteine, cysteine and histidine and negatively charged amino acids such as Glu and Asp. Also, aromatic residues such as tyrosine and tryptophan strongly interact with Au(III). In proteins, basic pH plays a role in the deprotonation of cysteine, lysine and tyrosine and strongly increases the binding affinity of Au(III) toward these amino acids. The amino acid residues in the protein can also trigger the reduction of Au(III) ions. Sulfur-containing amino acids (cysteine and methionine) and selenocysteine provide almost one electron to Au(III) upon binding. Tyrosine also shows a considerable tendency to act as a reductant. Other amino acids, commonly identified in Au-protein adducts, such as Ser, Trp, Thr, Gln, Glu, Asn, Asp, Lys, Arg and His, possess a notable reducing power toward Au(III). These results and their discussion form a vade mecum that can find application in medicinal chemistry and nanotech applications of Au(III).- Published
- 2024
- Full Text
- View/download PDF
35. Recurrent incisional hernia, enterocutaneous fistula and loss of the substance of the abdominal wall: plastic with organic prosthesis, skin graft and VAC therapy. Clinical case.
- Author
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Nicodemi S, Corelli S, Sacchi M, Ricciardi E, Costantino A, Di Legge P, Ceci F, Cipriani B, Martellucci A, Santilli M, Orsini S, Tudisco A, and Stagnitti F
- Subjects
- Body Mass Index, Collagen administration & dosage, Female, Hernia, Abdominal complications, Hernia, Abdominal surgery, Humans, Incisional Hernia complications, Incisional Hernia pathology, Intestinal Fistula etiology, Middle Aged, Skin Transplantation methods, Treatment Outcome, Incisional Hernia surgery, Intestinal Fistula surgery, Negative-Pressure Wound Therapy methods, Obesity complications, Surgical Mesh
- Abstract
Surgical wounds dehiscence is a serious post-operatory complication, with an incidence between 0.4% and 3.5%. Mortality is more than 45%. Complex wounds treatment may require a multidisciplinary management. VAC Therapy could be an alternative treatment regarding complex wound. VAC therapy has been recently introduced on skin's graft tissue management reducing skin graft rejection. The use of biological prosthesis has been tested in a contaminated field, better than synthetic meshes, which often need to be removed. The Permacol is more resistant to degradation by proteases due to its cross-links. Surgery is still considered the best treatment for digestive fistula. A 58 years old obese woman come to our attention, she was operated for an abdominal hernia. She had a post-operatory entero-cutaneous fistula. She was submitted to bowel resection, the anastomosis has been tailored and the hernia of the abdominal wall has been repaired with biological mesh for managing such condition. She had a wound dehiscence with loss of substance and the exposure of the biological prosthesis, nearly 20 cm diameter. She was treated first with antibiotic therapy and simple medications. In addiction, antibiotic therapy was necessary late associated to 7 months with advanced medications allowed a small reduction's defect. Because of its, treatment went on for two more months using VAC therapy. Antibiotic's therapy was finally suspended. The VAC therapy allowed the reduction of the gap, between skin and subcutaneous tissue, and the defect's size preparing a suitable ground for the skin graft. The graft, managed with the vac therapy, was necessary to complete the healing process.
- Published
- 2015
36. Small bowel intussussception due to metastatic melanoma of unknown primary site. Case report.
- Author
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Stagnitti F, Orsini S, Martellucci A, Tudisco A, Avallone M, Aiuti F, Di Girolamo V, Stefanelli F, De Angelis F, Di Grazia C, Napoleoni A, Nicodemi S, Cipriani B, Ceci F, Mosillo R, Corelli S, Casciaro G, and Spaziani E
- Subjects
- Humans, Male, Middle Aged, Ileal Neoplasms complications, Ileal Neoplasms secondary, Intestinal Obstruction etiology, Melanoma complications, Melanoma secondary, Neoplasms, Unknown Primary pathology
- Abstract
Malignant melanoma is characterized by metastases also to the gastrointestinal tract, especially in the small bowel. The diagnosis is often delayed because unspecific clinical presentation (frequently as chronic iron deficiency anemia, rectal bleeding or intestinal obstruction). We present a case of melanoma of unknown primary site, with clinical presentation of intestinal obstruction. A segmental resection of the ileum was performed including mesentery with lymph nodes. Histology revealed metastatic melanoma from unknown primary. PET and MRI confirmed disseminated disease without brain metastasis.
- Published
- 2014
37. Biliodigestive fistulae and gallstone ileus: diagnostic and therapeutic considerations. Our experience.
- Author
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Stagnitti F, Tudisco A, Ceci F, Nicodemi S, Orsini S, Avallone M, Di Girolamo V, Stefanelli F, De Angelis F, Di Grazia C, Cipriani B, Aiuti F, Napoleoni A, Mosillo R, Corelli S, Casciaro G, Costantino A, Martellucci A, and Spaziani E
- Subjects
- Adolescent, Aged, Aged, 80 and over, Female, Gallstones complications, Humans, Ileus etiology, Male, Middle Aged, Biliary Fistula diagnosis, Biliary Fistula surgery, Gallstones diagnosis, Gallstones surgery, Ileus diagnosis, Ileus surgery, Intestinal Fistula diagnosis, Intestinal Fistula surgery
- Abstract
The biliodigestive fistula is not a rare affection in the context of acute pathology of the gastrointestinal tract. It often affects patients between 63 and 85 years old , particularly the female sex, and the most common cause is acute or chronic cholecystolithiasis. Open issues are the delayed in the pre-operative diagnosis, and controversies exist regarding the best surgical approach. The choice of treatment options is influenced by the age of the patients and their clinical conditions and also by the presence of comorbidities and of a delayed right diagnosis. In the 1 to 3% of cases, the biliodigestive fistula presents a gallstone ileus as complication, whose diagnosis is particularly difficult for the lack of specific signs and symptoms. The contrast-enhanced CT is considered the gold standard for a specific pre-operative diagnosis, as it directly shows the fistula. Surgical treatments include one-stage procedure or two-stage procedure. Many studies seem to favor a deferred definitive procedure. The Authors describe 4 cases: in 3 cases, women between 70 and 80 years old presenting an history of recurrent cholecystitis, in 2 cases, and in 1 case presenting a bowel obstruction; in 1 case a 50-years-old man, with no significant past medical history, presenting a bowel obstruction. The Authors have performed in the 2 cases of gallstone ileus an enterolithotomy with cholecysto-duodenal fistula repair and cholecystectomy, in one-stage, and this has been possible because of the good clinical conditions of the patients and their low operative risk. In the case of fistula without the complication of gallstone ileus, the treatment approach has been cholecysto-gastric fistula closure with a gastroplastic using separate stitches and cholecystectomy, in one-stage. We are in agreement with data in the literature regarding the delay into the diagnosis of biliodigestive fistula and with the importance to suspect it or gallstone ileus presence, although the clinical presentation is extremely non-specific. In our experience, cholangiopancreatography-CT and CECT have made easier the pre-operative diagnosis and so reducing the delay of the treatment.
- Published
- 2014
38. Single-incision laparoscopic appendectomy is comparable to conventional laparoscopic and laparotomic appendectomy: our single center single surgeon experience.
- Author
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Ceci F, Orsini S, Tudisco A, Avallone M, Aiuti F, Di Girolamo V, Stefanelli F, De Angelis F, Martellucci A, Costantino A, Di Grazia C, Nicodemi S, Cipriani B, Napoleoni A, Mosillo R, Corelli S, Casciaro G, Spaziani E, and Stagnitti F
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Female, Humans, Male, Middle Aged, Retrospective Studies, Young Adult, Appendectomy methods, Laparoscopy methods, Laparotomy
- Abstract
Several studies have demonstrated the clinical and technical benefits of the laparoscopic surgery for complicated and uncomplicated appendicitis. Our retrospective study included 12 patient who underwent SILS appendectomy (SILS-A), 14 who received conventional laparoscopic surgery (VL-A), and 12 who received laparotomic appendectomy (OA); performed in all cases by the same surgeon (C.F.). The aim of this study was the comparison between this three different surgical techniques on same features: post operative leukocytosis, post operative pain, need abdominal drainage, esthetic viewpoint, incidence of complication, hospital stay. The results showed no significant differences between SILS-A and VLS-A, while an evident improvement shows versus O-A, even though not statistically significative. SILS was more effective in decreasing the risk of postoperative wound infection.
- Published
- 2013
- Full Text
- View/download PDF
39. Technique and outcomes about a new laparoscopic procedure: the Pelvic Organ Prolapse Suspension (POPS).
- Author
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Ceci F, Spaziani E, Corelli S, Casciaro G, Martellucci A, Costantino A, Napoleoni A, Cipriani B, Nicodemi S, Di Grazia C, Avallone M, Orsini S, Tudisco A, Aiuti F, and Stagnitti F
- Subjects
- Female, Follow-Up Studies, Gynecologic Surgical Procedures methods, Humans, Middle Aged, Treatment Outcome, Laparoscopy, Pelvic Organ Prolapse surgery
- Abstract
Pelvic organ prolapse suspension (POPS) is a recent surgical procedure for one-stage treatment of multiorgan female pelvic prolapse. This study evaluates the preliminary results of laparoscopic POPS in 54 women with a mean age of 55.2 and a BMI of 28.3. Patients underwent at the same time stapled transanal rectal resection (STARR) to correct the residual rectal prolapse. We had no relapses and the preliminary results were excellent. We evaluated the patients after 1 year follow-up and we confirmed the validity of our treatment. The technique is simpler than traditional treatments with an important reduction or completely disappearance of the pre-operative symptomatology.
- Published
- 2013
- Full Text
- View/download PDF
40. Cholecystectomy by single incision laparoscopic surgery (SILS): early experience and technique standardization.
- Author
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Ceci F, Di Grazia C, Cipriani B, Nicodemi S, Corelli S, Pecchia M, Martellucci A, Costantino A, Stefanelli F, Salvadori C, Napoleoni A, Parisella M, Spaziani E, and Stagnitti F
- Subjects
- Adult, Aged, Female, Humans, Male, Middle Aged, Young Adult, Cholecystectomy, Laparoscopic methods, Cholecystectomy, Laparoscopic standards
- Abstract
Single Incision Laparoscopic Surgery (SILS) is a recent surgical technique, first described in the 1990s. Its aim is to optimize the esthetic result offered by laparoscopy by minimizing the number of abdominal incisions. Various preliminary studies have been carried out on the application of SILS, especially in cholecystectomy and appendectomy. This study evaluates the preliminary results of cholecystectomy by SILS (SILS™ Port) conducted between October 2009 and February 2011 on 21 patients (4 men and 17 women) with a mean age of 49.9 years and a mean Body Mass Index (BMI) of 22.8. All patients were treated by the same team, which had previously undergone six months' simulator training. There were two main selection criteria, both evaluated intraoperatively: absence of adhesions and of significant inflammatory sequelae from previous cholecystitis; and suitable distance between gallbladder and SILS access port. Conversion to traditional laparoscopy was necessary in just two cases, while an accessory trocar was introduced in another two cases. Conversion to open surgery was not necessary in any case. One case of SILS cholecystectomy was complicated by postoperative bile leakage, which was treated conservatively, as the fistula had a low output. The mean duration of hospitalization was 3.6 days. This preliminary experience led us to conclude that SILS is safe and highly satisfactory in the postoperative phase, thanks to the reduced need for painkillers and the improved esthetic result.
- Published
- 2012
41. Liver resection for hemoperitoneum caused by spontaneous rupture of unrecognized hepatocellular carcinoma.
- Author
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Casciaro GE, Spaziani E, Costantino A, Ceci F, Di Grazia C, Martellucci A, Pecchia M, Cipriani B, De Angelis F, Corelli S, Napoleoni A, Stefanelli F, Salvadori C, Parisella M, Nicodemi S, and Stagnitti F
- Subjects
- Aged, 80 and over, Humans, Male, Rupture, Spontaneous, Carcinoma, Hepatocellular complications, Carcinoma, Hepatocellular surgery, Hemoperitoneum etiology, Hemoperitoneum surgery, Hepatectomy, Liver Neoplasms complications, Liver Neoplasms surgery
- Abstract
Hepatocellular carcinoma (HCC) is an increasingly common form of cancer. Although its spontaneous rupture is rare in Western countries, it constitutes a surgical emergency and is associated with high mortality. There is a lack of consensus as to the best approach and what parameters to use in choosing it. The three main approaches are conservative, endovascular and resection - the treatment of choice for acute abdominal bleeding. We report a case of hemoperitoneum following the spontaneous rupture of an unrecognized HCV-related HCC in a patient with no history of liver disease. The patient was successfully treated by emergency surgery, with resection of two segments of the left liver.
- Published
- 2012
42. A TLR9 agonist enhances therapeutic effects of telomerase genetic vaccine.
- Author
-
Conforti A, Cipriani B, Peruzzi D, Dharmapuri S, Kandimalla ER, Agrawal S, Mori F, Ciliberto G, La Monica N, and Aurisicchio L
- Subjects
- Adaptive Immunity, Adenoviridae, Adjuvants, Immunologic genetics, Animals, Cancer Vaccines genetics, Cytokines immunology, Cytokines metabolism, Dendritic Cells immunology, Electroporation, Female, Immunity, Innate, Killer Cells, Natural immunology, Melanoma, Experimental immunology, Melanoma, Experimental prevention & control, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Oligonucleotides immunology, T-Lymphocytes immunology, Vaccines, DNA genetics, Xenograft Model Antitumor Assays, Cancer Vaccines immunology, Telomerase immunology, Toll-Like Receptor 9 agonists, Vaccines, DNA immunology
- Abstract
The telomerase reverse transcriptase (TERT) is an attractive target for cancer vaccination because its expression is reactivated in most tumors. In this study, we have evaluated the ability of a genetic vaccine targeting murine TERT (mTERT) based on DNA electroporation (DNA-EP) and adenovirus serotype 6 (Ad6) to exert therapeutic effects in combination with a novel TLR9 agonist, referred to as immune modulatory oligonucleotide (IMO), as an adjuvant. IMO was administered to mice at the same time as vaccine. IMO induced dose-dependent cytokine secretion and activation of NK cells. Most importantly, vaccination of mice with IMO in combination with mTERT vaccine conferred therapeutic benefit in tumor bearing animals and this effect was associated with increased NK, DC and T cell tumor infiltration. These data show that appropriate combination of a DNA-EP/Ad6-based cancer vaccine against TERT with IMO induces multiple effects on innate and adaptive immune responses resulting in a significant antitumor efficacy., ((c) 2010 Elsevier Ltd. All rights reserved.)
- Published
- 2010
- Full Text
- View/download PDF
43. Autoimmune B-cell lymphopenia after successful adoptive therapy with telomerase-specific T lymphocytes.
- Author
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Ugel S, Scarselli E, Iezzi M, Mennuni C, Pannellini T, Calvaruso F, Cipriani B, De Palma R, Ricci-Vitiani L, Peranzoni E, Musiani P, Zanovello P, and Bronte V
- Subjects
- Adenocarcinoma immunology, Animals, B-Lymphocytes immunology, B-Lymphocytes pathology, Bone Marrow Cells pathology, Cancer Vaccines, Cell Line, Tumor, Colonic Neoplasms, Disease Models, Animal, HLA-A2 Antigen genetics, HLA-A2 Antigen immunology, Lung Neoplasms, Male, Melanoma, Mice, Mice, Inbred C57BL, Mice, Transgenic, Neoplasm Transplantation, Prostatic Neoplasms immunology, Skin Neoplasms, Spleen pathology, T-Lymphocytes immunology, Adenocarcinoma therapy, Adoptive Transfer methods, Lymphopenia pathology, Prostatic Neoplasms therapy, T-Lymphocytes transplantation, Telomerase immunology
- Abstract
Telomerase reverse transcriptase (TERT) is a good candidate for cancer immunotherapy because it is overexpressed in 85% of all human tumors and implicated in maintenance of the transformed phenotype. TERT-based cancer vaccines have been shown to be safe, not inducing any immune-related pathology, but their impact on tumor progression is modest. Here we show that adoptive cell therapy with the use of high-avidity T lymphocytes reactive against telomerase can control the growth of different established tumors. Moreover, in transgenic adenocarcinoma mouse prostate mice, which develop prostate cancer, TERT-based adoptive cell therapy halted the progression to more aggressive and poorly differentiated tumors, significantly prolonging mouse survival. We also demonstrated that human tumors, including Burkitt lymphoma, and human cancer stem cells, are targeted in vivo by TERT-specific cytotoxic T lymphocytes. Effective therapy with T cells against telomerase, different from active vaccination, however, led to autoimmunity marked by a consistent, although transient, B-cell depletion in primary and secondary lymphoid organs, associated with alteration of the spleen cytoarchitecture. These results indicate B cells as an in vivo target of TERT-specific cytotoxic T lymphocytes during successful immunotherapy.
- Published
- 2010
- Full Text
- View/download PDF
44. In vivo administration of artificial antigen-presenting cells activates low-avidity T cells for treatment of cancer.
- Author
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Ugel S, Zoso A, De Santo C, Li Y, Marigo I, Zanovello P, Scarselli E, Cipriani B, Oelke M, Schneck JP, and Bronte V
- Subjects
- Animals, Antibody Affinity, Cell Growth Processes immunology, Female, Leukemia, Experimental immunology, Lymphocyte Activation, Melanoma, Experimental immunology, Mice, Mice, Inbred C57BL, T-Lymphocytes, Cytotoxic immunology, Antigen-Presenting Cells immunology, Immunotherapy, Adoptive methods, Leukemia, Experimental therapy, Melanoma, Experimental therapy, T-Lymphocytes immunology
- Abstract
The development of effective antitumor immune responses is normally constrained by low-avidity, tumor-specific CTLs that are unable to eradicate the tumor. Strategies to rescue antitumor activity of low-avidity melanoma-specific CTLs in vivo may improve immunotherapy efficacy. To boost the in vivo effectiveness of low-avidity CTLs, we immunized mice bearing lung melanoma metastases with artificial antigen-presenting cells (aAPC), made by covalently coupling (pep)MHC-Ig dimers and B7.1-Ig molecules to magnetic beads. aAPC treatment induced significant tumor reduction in a mouse telomerase antigen system, and complete tumor eradication in a mouse TRP-2 antigen system, when low-avidity CTLs specific for these antigens were adoptively transferred. In addition, in an in vivo treatment model of subcutaneous melanoma, aAPC injection also augmented the activity of adoptively transferred CTLs and significantly delayed tumor growth. In vivo tumor clearance due to aAPC administration correlated with in situ proliferation of the transferred CTL. In vitro studies showed that aAPC effectively stimulated cytokine release, enhanced CTL-mediated lysis, and TCR downregulation in low-avidity CTLs. Therefore, in vivo aAPC administration represents a potentially novel approach to improve cancer immunotherapy.
- Published
- 2009
- Full Text
- View/download PDF
45. Immunological markers of cancer vaccine efficacy and their clinical relevance.
- Author
-
Cipriani B
- Abstract
As the field of anticancer therapy advances, with a mission to either prevent tumors or improve the quality of life and survival of those affected by the disease, biomarker discovery in oncology is also growing. Since cancer therapy has become a type of personalized medicine, biomarkers are strongly needed in order to identify the right therapy for the right patient and predict efficacy in a timely manner so that alternative approaches can be tested. In this article, the author summarizes the most recent studies published on cancer vaccines where immunological parameters were analyzed to assess immunogenicity and, most importantly, to establish correlations with clinical benefit. As highlighted here, the complexity of both the disease and the immune system still makes it a very challenging discovery process.
- Published
- 2009
- Full Text
- View/download PDF
46. [Umbilical primary endometrioma. Case report].
- Author
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Spaziani E, Di Filippo A, Picchio M, Briganti M, De Cristofano C, Ceci F, Martellucci A, Cipriani B, Nardecchia G, De Angelis F, Iorio O, Nicodemi S, Pattaro G, and Stagnitti F
- Subjects
- Adult, Diagnosis, Differential, Endometriosis diagnosis, Endometriosis surgery, Female, Humans, Treatment Outcome, Umbilicus surgery, Endometriosis pathology, Umbilicus pathology
- Abstract
Umbilical primary endometrioma is a rare extra-uterine localization of endometriosis with a documented neoplastic risk. It is often difficult to distinguish primary umbilical endometriosis from other benign and malignant tumors of the abdominal wall. The Authors report a case of umbilical endometriosis in a 36-year old female. Endometriosis was suspected because of the presence of the typical cyclic bleeding and swelling. Abdominal CT excluded the presence of other endometriotic localizations. The umbilical mass was widely excised together with umbilicus, fascia and peritoneum. The tissue defect was primary closed without prosthetic mesh. Histological examination of the specimen showed the presence of endometrial glands with stromal component, compatible with the diagnosis of endometriosis. The Authors suggest that surgery should be performed in the first 3-4 days of the follicular phase, to minimize the risk of diffusion of endometriotic cells. Moreover, the excision should be wide in order to prevent local recurrence.
- Published
- 2009
47. ErbB2 genetic cancer vaccine in nonhuman primates: relevance of single nucleotide polymorphisms.
- Author
-
Fattori E, Aurisicchio L, Zampaglione I, Arcuri M, Cappelletti M, Cipriani B, Mennuni C, Calvaruso F, Nuzzo M, Ciliberto G, Monaci P, and La Monica N
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, Female, Humans, Immunity, Cellular, Immunization, Secondary, Macaca mulatta, Mice, Molecular Sequence Data, Recombinant Proteins biosynthesis, Self Tolerance, Sequence Analysis, DNA, Genes, erbB-2, Neoplasms, Glandular and Epithelial genetics, Neoplasms, Glandular and Epithelial immunology, Neoplasms, Glandular and Epithelial therapy, Polymorphism, Single Nucleotide, Vaccines, DNA therapeutic use
- Abstract
Aberrant Her2/neu expression is associated with the development of epithelial-derived human carcinomas and for this reason it is considered a good target for immunologic intervention. To define methods to circumvent immunologic tolerance and to elicit immunity against the Her2/neu tumor-associated antigen in a suitable animal model, we have isolated the cDNA encoding the rhesus monkey homolog of human Her2/neu (RhErbB2) to construct DNA plasmids and adenoviral vectors for the development of a cancer vaccine against this protein. To further increase the immunogenic potency of these vectors, a synthetic codon-optimized RhErbB2 cDNA (RhErbB2OPT) was constructed and characterized. Genetic vaccination of rhesus monkeys was effective in inducing a response against RhErbB2 in immunized animals; importantly, the elicited immunity was associated with natural RhErbB2 polymorphisms, thus distinguishing responses against "self " and "nonself " epitopes. In particular, the postpriming response recognized mainly nonself epitopes whereas the boosted response cross-reacted with self epitopes. Our findings are particularly relevant in the investigation of the impact of TAA polymorphisms on the efficacy of a cancer vaccine strategy.
- Published
- 2009
- Full Text
- View/download PDF
48. [Massive haemoperitoneum due to traumatic rupture of multifocal hepatocarcinoma in the right hepatic lobe. Case report].
- Author
-
Spaziani E, Briganti M, Saltarelli A, Iozzino M, Notarianni E, Cianni R, Di Filippo A, Picchio M, Ceci F, Gammardella P, De Angelis F, Nardecchia G, Cipriani B, Nicodemi S, and Stagnitti F
- Subjects
- Aged, 80 and over, Bandages, Carcinoma, Hepatocellular complications, Carcinoma, Hepatocellular diagnosis, Carcinoma, Hepatocellular surgery, Combined Modality Therapy, Embolization, Therapeutic, Fatal Outcome, Female, Hemoperitoneum therapy, Hemostasis, Surgical, Hepatic Artery diagnostic imaging, Humans, Laparotomy, Liver Neoplasms complications, Liver Neoplasms diagnosis, Liver Neoplasms surgery, Postoperative Complications, Pressure, Radiography, Respiratory Insufficiency, Rupture complications, Rupture surgery, Rupture therapy, Tissue Adhesives, Hemoperitoneum etiology
- Abstract
Unlabelled: Abdominal blunt trauma is the main cause of death in people younger than 40 years old. The liver injury still represents a challenging problem. Isolated hepatic injury is rare and it occurs more frequentely in polytraumatizated patients and causes massive haemoperitoneum. The Authors report a case of a 83 years-old woman admitted to Emergency Department for syncope due to an active bleeding arising from a rupture of a right hepatic lobe unsuspected tumor. The computer tomography (CT) scans showed a clear pattern of liver laceration of the VI segment with contrast enhancement spreading in the surrounding tissues, and detected a multifocal hepatocarcinoma located in the VI, VII and VIII segments. Patient's haemodinamically unstable conditions suggested an urgent laparotomy. An accurate perihepatic packing with sterile-drape were successfully employed to control liver hemorrage. Temporary abdominal closure, followed by hepatic arteriography and the right hepatic artery embolization, completed the damage control. Re-exploration laparotomy after 72 hours confirmed the definitive haemostasis and the pack removal was performed without complications., Conclusions: CT plays a leading role in the diagnosis of liver damage. The patient's haemodynamic status is the principal criterion determining conservative or operative therapy in blunt liver injury. The early perihepatic packing followed by artheriographic embolization to stop liver hemorrhage showed efficacy and safety for the patient. The packing performed with sterile-drape is able to avoid removal complications and 72 hours timing for the pack removal is effective to avoid re-bleeding.
- Published
- 2009
49. Preventive vaccination with telomerase controls tumor growth in genetically engineered and carcinogen-induced mouse models of cancer.
- Author
-
Mennuni C, Ugel S, Mori F, Cipriani B, Iezzi M, Pannellini T, Lazzaro D, Ciliberto G, La Monica N, Zanovello P, Bronte V, and Scarselli E
- Subjects
- Animals, CD8-Positive T-Lymphocytes immunology, Cancer Vaccines genetics, Cancer Vaccines pharmacology, Colonic Neoplasms enzymology, Colonic Neoplasms prevention & control, Disease Models, Animal, Epitopes, T-Lymphocyte immunology, Female, Genetic Engineering, Lymphocytes, Tumor-Infiltrating immunology, Male, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Prostatic Neoplasms enzymology, Prostatic Neoplasms prevention & control, Telomerase genetics, Vaccines, DNA genetics, Vaccines, DNA pharmacology, Cancer Vaccines immunology, Colonic Neoplasms immunology, Prostatic Neoplasms immunology, Telomerase immunology, Vaccines, DNA immunology
- Abstract
The telomerase reverse transcriptase, TERT, is an attractive target for human cancer vaccination because its expression is reactivated in a conspicuous fraction of human tumors. Genetic vaccination with murine telomerase (mTERT) could break immune tolerance in different mouse strains and resulted in the induction of both CD4+ and CD8+ telomerase-specific T cells. The mTERT-derived immunodominant epitopes recognized by CD8+ T cells were further defined in these mouse strains and used to track immune responses. Antitumor efficacy of telomerase-based vaccination was investigated in two cancer models closely resembling human diseases: the TRAMP transgenic mice for prostate cancer and a carcinogen-induced model for colon cancer. TERT overexpression in tumor lesions was shown in both models by immunohistochemistry, thus reinforcing the similarity of these tumors to their human counterparts. Repeated immunizations with mTERT-encoding DNA resulted in a significant delay of tumor formation and progression in both the prostate cancer and the colon cancer models. Moreover, evaluation of the intratumoral infiltrate revealed the presence of telomerase-specific T cells in vaccinated mice. The safety of vaccination was confirmed by the absence of histomorphologic changes on postnecropsy analysis of several organs and lack of adverse effects on blood cell counts. These results indicate that TERT vaccination can elicit antigen-specific immunosurveillance and imply this antigen as a potential candidate for preventive cancer vaccines.
- Published
- 2008
- Full Text
- View/download PDF
50. [A case of arterial chemoembolization for hepatocellular carcinoma with 11 years of survival].
- Author
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Iozzino M, Spaziani E, Saltarelli A, Notarianni E, Di Filippo A, Picchio M, Ceci F, De Angelis F, Cipriani B, Nardecchia G, Nicodemi S, Stagnitti F, and Cianni R
- Subjects
- Antineoplastic Agents, Hormonal administration & dosage, Carcinoma, Hepatocellular pathology, Catheter Ablation methods, Humans, Liver Neoplasms pathology, Male, Middle Aged, Tamoxifen administration & dosage, Treatment Outcome, Carcinoma, Hepatocellular therapy, Chemoembolization, Therapeutic, Liver Neoplasms therapy
- Abstract
Hepatocellular carcinoma (HCC) is the most common malignancy of the liver and the third most common cause of cancer mortality worldwide. The major risk of developing HCC is associated with HBV and HCV hepatitis. Liver transplant (LT) is the gold standard for "small" HCC (HCCs) in Child-Pugh class A cirrhotic patients. However its use has been restricted by the severe shortage of donors, so that hepatic resection (HR) is often performed in these patients. In the last two decades image-guided interventional catheterization and ablative regional treatment procedures have revolutionized the therapy of unresectable primary and secondary liver tumors. The Authors present a case of a 61-years old man with Child-Pugh class A HCCs. The age and the previous history of bladder carcinoma made the patient not suitable for LT. The patient refused HR so that transarterial chemoembolization combined to thermo-ablation therapy and oral intake of tamoxifen were proposed. Patient's tolerance to the treatments has been good. During 11-year follow-up there was earlier intrahepatic progression of the tumor followed by reduction in size and number of the lesions. In spite of the scarce prognosis, chemoembolization and immunotherapy allowed to achieve a satisfactory local control of disease in our patient and guaranteed a good quality of life at long-term follow-up.
- Published
- 2008
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