1. A duplicated copy of id2b is an unusual sex-determining candidate gene on the Y chromosome of arapaima (Arapaima gigas)
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Adolfi, Mateus C., Du, Kang, Kneitz, Susanne, Cabau, Cédric, Zahm, Margot, Klopp, Christophe, Feron, Romain, Paixão, Rômulo V., Varela, Eduardo S., de Almeida, Fernanda L., de Oliveira, Marcos A., Nóbrega, Rafael H., Lopez-Roques, Céline, Iampietro, Carole, Lluch, Jérôme, Kloas, Werner, Wuertz, Sven, Schaefer, Fabian, Stöck, Matthias, Guiguen, Yann, Schartl, Manfred, University of Würzburg = Universität Würzburg, Texas State University, Système d'Information des GENomes des Animaux d'Elevage (SIGENAE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de Mathématiques et Informatique Appliquées de Toulouse (MIAT INRA), Laboratoire de Physiologie et Génomique des Poissons (LPGP), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institute of Sport Sciences of University of Lausanne (ISSUL), Swiss Institute of Bioinformatics [Lausanne] (SIB), Université de Lausanne (UNIL), EMBRAPA Amazônia Oriental, Embrapa Pesca E Aquicultura, Universidade Estadual Paulista Júlio de Mesquita Filho = São Paulo State University (UNESP), Génome et Transcriptome - Plateforme Génomique ( GeT-PlaGe), Plateforme Génome & Transcriptome (GET), Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Leibniz Institute of Freshwater Ecology and Inland Fisheries (IGB), Hiroshima University, University Hospital of Würzburg, University of Wuerzburg, Université de Toulouse, LPGP, and Swiss Institute of Bioinformatics, Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), Universidade Estadual Paulista (UNESP), Genotoul, IGB, University Hospital, Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), MATEUS C. ADOLFI, University of Wuerzburg, KANG DU, University of Wuerzburg, SUSANNE KNEITZ, University of Wuerzburg, CÉDRIC CABAU, Université de Toulouse, MARGOT ZAHM, Université de Toulouse, CHRISTOPHE KLOPP, INRA, ROMAIN FERON, INRAE, RÔMULO V. PAIXÃO, Bolsista CPAA, EDUARDO SOUSA VARELA, CNPASA, FERNANDA LOUREIRO ALMEIDA OSULLIVAN, CPAA, MARCOS A. DE OLIVEIRA, UNESP, RAFAEL H. NÓBREGA, UNESP, CÉLINE LOPEZ-ROQUES, INRAE, CAROLE IAMPIETRO, INRAE, JÉRÔME LLUCH, INRAE, WERNER KLOAS, Institute of Freshwater Ecology and Inland Fisheries, SVEN WUERTZ, Institute of Freshwater Ecology and Inland Fisheries, FABIAN SCHAEFER, Institute of Freshwater Ecology and Inland Fisheries, MATTHIAS STÖCK, Institute of Freshwater Ecology and Inland Fisheries, YANN GUIGUEN, INRAE, and MANFRED SCHARTL, Comprehensive Cancer Center Mainfranken.
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Genetic Markers ,Male ,Conservation of Natural Resources ,Genotype ,Science ,[SDV]Life Sciences [q-bio] ,Fisheries ,Polymerase Chain Reaction ,Article ,Evolutionary genetics ,Protein Structure, Secondary ,Arapaima gigas ,Evolution, Molecular ,Pirarucu ,Gene Duplication ,Y Chromosome ,Animals ,ddc:610 ,Phylogeny ,Inhibitor of Differentiation Protein 2 ,Genome ,Sex Chromosomes ,Fishes ,Chromosome Mapping ,DNA ,Sequence Analysis, DNA ,Protein Structure, Tertiary ,Phenotype ,Medicine ,Female - Abstract
Made available in DSpace on 2022-04-28T19:46:56Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-12-01 Julius-Maximilians-Universität Würzburg Arapaima gigas is one of the largest freshwater fish species of high ecological and economic importance. Overfishing and habitat destruction are severe threats to the remaining wild populations. By incorporating a chromosomal Hi-C contact map, we improved the arapaima genome assembly to chromosome-level, revealing an unexpected high degree of chromosome rearrangements during evolution of the bonytongues (Osteoglossiformes). Combining this new assembly with pool-sequencing of male and female genomes, we identified id2bbY, a duplicated copy of the inhibitor of DNA binding 2b (id2b) gene on the Y chromosome as candidate male sex-determining gene. A PCR-test for id2bbY was developed, demonstrating that this gene is a reliable male-specific marker for genotyping. Expression analyses showed that this gene is expressed in juvenile male gonads. Its paralog, id2ba, exhibits a male-biased expression in immature gonads. Transcriptome analyses and protein structure predictions confirm id2bbY as a prime candidate for the master sex-determiner. Acting through the TGFβ signaling pathway, id2bbY from arapaima would provide the first evidence for a link of this family of transcriptional regulators to sex determination. Our study broadens our current understanding about the evolution of sex determination genetic networks and provide a tool for improving arapaima aquaculture for commercial and conservation purposes. Developmental Biochemistry Biocenter University of Wuerzburg, Am Hubland The Xiphophorus Genetic Stock Center Department of Chemistry and Biochemistry Texas State University, San Marcos Biochemistry and Cell Biology Biocenter University of Wuerzburg, Am Hubland Sigenae GenPhySE INRAE ENVT Université de Toulouse MIAT INRA Université de Toulouse, Chemin de Borde Rouge INRAE LPGP Department of Ecology and Evolution University of Lausanne and Swiss Institute of Bioinformatics Embrapa Amazônia Ocidental Embrapa Pesca E Aquicultura Reproductive and Molecular Biology Group Department of Morphology Institute of Biosciences UNESP GeT-PlaGe INRAE Genotoul Leibniz-Institute of Freshwater Ecology and Inland Fisheries IGB, Müggelseedamm 301 & 310 Amphibian Research Center Hiroshima University Comprehensive Cancer Center Mainfranken University Hospital Reproductive and Molecular Biology Group Department of Morphology Institute of Biosciences UNESP
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- 2021