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Andrographolide (Andro) has emerged recently as a potential and effective anticancer agent with induction of apoptosis in some cancer cell lines while induction of G2/M arrest with weak apoptosis in others. Few studies have proved that Andro is also effective in combination therapy. The flavonoid Taxifolin (Taxi) has showed anti-oxidant and antiproliferative effects against different cancer cells. Therefore, the present study investigated the cytotoxic effects of Andro alone or in combination with Taxi on HeLa cells. The combination of Andro with Taxi was synergistic at all tested concentrations and combination ratios. Andro alone induced caspase-dependent apoptosis which was enhanced by the combination with Taxi and attenuated partly by using Z-Vad-Fmk. Andro induced a protective reactive oxygen species (ROS)-dependent autophagy which was attenuated by Taxi. The activation of p53 was involved in Andro-induced autophagy where the use of Taxi or pifithrin-α (PFT-α) decreased it while the activation of JNK was involved in the cell death of HeLa cells but not in the induction of autophagy. The mitochondrial outer-membrane permeabilization (MOMP) plays an important role in Andro-induced cell death in HeLa cells. Andro alone increased the MOMP which was further increased in the case of combination. This led to the increase in AIF and cytochrome c release from mitochondria which consequently increased caspase-dependent and independent cell death. In conclusion, Andro induced a protective autophagy in HeLa cells which was reduced by Taxi and the cell death was increased by increasing the MOMP and subsequently the …

AL ZAHARNA, M & CHEUNG, HY 2014, 'Achieving Synergistic Effects by Combining Different Phytochemicals for the Prevention and Treatment of Cancer' Hong Kong Pharmaceutical Journal, vol 21, no. 1, pp. 20 - 24 … AL ZAHARNA, M., & CHEUNG, H. (2014). Achieving Synergistic Effects by Combining Different Phytochemicals for the Prevention and Treatment of Cancer. Hong Kong Pharmaceutical Journal, 21(1), 20 - 24 … AL ZAHARNA M, CHEUNG HY. Achieving Synergistic Effects by Combining Different Phytochemicals for the Prevention and Treatment of Cancer. Hong Kong Pharmaceutical Journal. 2014 Jan;21(1):20 - 24 … AL ZAHARNA, Mazen; CHEUNG, Hon-Yeung / Achieving Synergistic Effects by Combining Different Phytochemicals for the Prevention and Treatment of Cancer … In: Hong Kong Pharmaceutical Journal, Vol. 21, No. 1, 01.2014, p. 20 - 24.

ALQOUQA, IAS, AL ZAHARNA, MMM & CHEUNG, HY 2014, 'Anticancer effect of andrographolide on cell cycle, apoptosis and autophagy of human breast carcinoma cells' 10th International Postgraduate Symposium on Chinese medicine (ICMCM), China, 12/08/14 - 14/08/14 … ALQOUQA, IAS, AL ZAHARNA, MMM, & CHEUNG, HY (2014). Anticancer effect of andrographolide on cell cycle, apoptosis and autophagy of human breast carcinoma cells. 10th International Postgraduate Symposium on Chinese medicine (ICMCM), China … ALQOUQA IAS, AL ZAHARNA MMM, CHEUNG HY. Anticancer effect of andrographolide on cell cycle, apoptosis and autophagy of human breast carcinoma cells. 2014. 10th International Postgraduate Symposium on Chinese medicine (ICMCM), China … EL Qouqa, Iyad A AS ; AL ZAHARNA, Mazen MM ; CHEUNG, Hon Yeung. / Anticancer effect of andrographolide on cell cycle, apoptosis and autophagy of …

Abri cantoniensis (AC) is regarded as a potent folk medicine for jaundice, hepatitis for long history worldwide. Abri Mollis (AM) is an alternative species of AC. In the present study, the high performance liquid chromatography (HPLC) profile and antioxidant activities of these two species were compared. With the established HPLC fingerprint, 20 batches of AC and AM were efficiently distinguished. The antioxidant activities against DPPH radical of 20 batches of AC and AM were compared, and AC showed generally stronger antioxidant activities than AM, with IC50 value ranging from 14.19 to 65.06 μg/mL. Extract of AC was further separated into 4 fractions by serial extraction. Only ethyl acetate fraction (EF) and n-butanol fraction (BF) showed strong DPPH radical scavenging activities, with IC50 value of 48.32±4.70 and 62.72±3.03 μg/mL, respectively. These two fractions also showed potent cellular antioxidant …

DONG, W, SHEN, Q, AL ZAHARNA, M & CHEUNG, HY 2013, 'PROTEOMIC ANALYSIS OF GERMINATION-ASSOCIATED PROTEINS OF GEOBACILLUS STEAROTHERMOPHILUS' Paper presented at International Symposium on Translating Omics Science to Clinical Applications 2013 (TOSCA'13), China, 13/08/13 - 15/08/13, pp. 6 … DONG, W., SHEN, Q., AL ZAHARNA, M., & CHEUNG, HY. (2013). PROTEOMIC ANALYSIS OF GERMINATION-ASSOCIATED PROTEINS OF GEOBACILLUS STEAROTHERMOPHILUS. 6. Paper presented at International Symposium on Translating Omics Science to Clinical Applications 2013 (TOSCA'13), China. … DONG W, SHEN Q, AL ZAHARNA M, CHEUNG HY. PROTEOMIC ANALYSIS OF GERMINATION-ASSOCIATED PROTEINS OF GEOBACILLUS STEAROTHERMOPHILUS. 2013. Paper presented at International Symposium on Translating Omics Science to …

YANG, M, AL ZAHARNA, M & CHEUNG, HY 2013, 'ETHYL ACETATE FRACTION OF ABRI CANTONISIS HERBA INDUCES CELL CYCLE ARREST AT G2/M PHASE IN HUMAN CERVICAL CANCER CELL LINE HELA' Paper presented at International Symposium on Translating Omics Science to Clinical Applications 2013 (TOSCA'13), China, 13/08/13 - 15/08/13, pp. 10 … YANG, M., AL ZAHARNA, M., & CHEUNG, HY. (2013). ETHYL ACETATE FRACTION OF ABRI CANTONISIS HERBA INDUCES CELL CYCLE ARREST AT G2/M PHASE IN HUMAN CERVICAL CANCER CELL LINE HELA. 10. Paper presented at International Symposium on Translating Omics Science to Clinical Applications 2013 (TOSCA'13), China … YANG M, AL ZAHARNA M, CHEUNG HY. ETHYL ACETATE FRACTION OF ABRI CANTONISIS HERBA INDUCES CELL CYCLE ARREST AT G2/M PHASE IN HUMAN CERVICAL CANCER CELL LINE HELA. 2013. Paper …

CHEUNG, HY, DONG, W & AL ZAHARNA, M 2012, 'AFM evidence of inhibitory effects of alkaline glutaraldehyde on spore germination of Bacillus stearothermophilus' 5th European Spore Conference, United Kingdom, 16/04/12 - 19/04/12 … CHEUNG, HY, DONG, W., & AL ZAHARNA, M. (2012). AFM evidence of inhibitory effects of alkaline glutaraldehyde on spore germination of Bacillus stearothermophilus. 5th European Spore Conference, United Kingdom … CHEUNG HY, DONG W, AL ZAHARNA M. AFM evidence of inhibitory effects of alkaline glutaraldehyde on spore germination of Bacillus stearothermophilus. 2012. 5th European Spore Conference, United Kingdom … CHEUNG, Hon Yeung ; DONG, Wei ; AL ZAHARNA, Mazen. / AFM evidence of inhibitory effects of alkaline glutaraldehyde on spore germination of Bacillus stearothermophilus. 5th European Spore Conference, United Kingdom.