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At present, image analysis and digital core are the main approaches for porous media reconstruction modeling, and they are both based on the real pore skeleton physical structure of porous media. However, it is difficult to reconstruct the reservoir and seepage characteristics of the real samples because of the limitations of accuracy in characterization techniques (imaging). In order to solve this problem and break through the barriers caused by the lack of accuracy, Spin-echo serial peripheral interface sequence of low field nuclear magnetic resonance is used to test the saturated water rock core with spatially resolved T2 distributions. Based on the experimental results of 1D T2 distributions, a novel method for fine reconstruction modeling of porous media is proposed, and the porous media model reconstructed by this new method better reproduces the reservoir and seepage characteristics of the original samples. Taking some of the tested porous media cores (P58 and Y75) as examples, representative elementary volume (REV)-lattice Boltzmann method (LBM) is used to simulate the flow field. Ensuring that the error of standard case is only 0.36% when multi-relaxation time REV-LBM is used, the distribution of porosity and permeability have been calculated and compared with the experimental data. The overall permeability error of the reconstructed porous media model is only 6.15 and 7.60%, respectively. Furthermore, the porosity and permeability error of almost all measuring points can be maintained within 3 and 8%. In addition, this method improves the efficiency of the existing reconstruction modeling methods, reduces the test cost, and makes the reconstruction modeling of porous media easier to operate, which has promising development prospects.

In the Synthetic Aperture Radar (SAR) remote sensing imagery of complicated scenes (especially urban scenes), there are a large number of lines and surfaces, such as roads in urban areas and the surfaces of buildings. These microwave-signal-scattering features have strong directivity. Traditional SAR acquires the scattering information of a scene from a single observation, and traditional imaging algorithms are based on the point target model, which makes the main features of the lines and surfaces in traditional SAR images appear as a series of strong scattering points rather than line-scattering and surface-scattering features. This outcome ultimately causes the target to be discontinuous in the SAR image, thus making the SAR image difficult to interpret. Therefore, in this study, we conducted an in-depth and meticulous investigation of the SAR imaging mechanism for lines and surfaces by establishing a parametric echo model of typical lines and triangular surfaces. Based on the proposed parametric model, we performed parametric imaging of these lines and surfaces. Based on our results, we propose a parametric imaging method, in which the typical lines and surfaces are classified and determined based on Bayesian theory and the proposed parametric model. Then, an SAR image can be obtained that effectively characterizes the scattering features of the line and surface targets by visual imaging, which effectively facilitates SAR image interpretation. The results of our numerical simulation experiments verify the validity of the proposed method.

Abstract Background Currently there exists a limited availability of genetic marker resources in sweetpotato (Ipomoea batatas), which is hindering genetic research in this species. It is necessary to develop more molecular markers for potential use in sweetpotato genetic research. With the newly developed next generation sequencing technology, large amount of transcribed sequences of sweetpotato have been generated and are available for identifying SSR markers by data mining. Results In this study, we investigated 181,615 ESTs for the identification and development of SSR markers. In total, 8,294 SSRs were identified from 7,163 SSR-containing unique ESTs. On an average, one SSR was found per 7.1 kb of EST sequence with tri-nucleotide motifs (42.9%) being the most abundant followed by di- (41.2%), tetra- (9.2%), penta- (3.7%) and hexa-nucleotide (3.1%) repeat types. The top five motifs included AG/CT (26.9%), AAG/CTT (13.5%), AT/TA (10.6%), CCG/CGG (5.8%) and AAT/ATT (4.5%). After removing possible duplicate of published EST-SSRs of sweetpotato, a total of non-repeat 7,958 SSR motifs were identified. Based on these SSR-containing sequences, 1,060 pairs of high-quality SSR primers were designed and used for validation of the amplification and assessment of the polymorphism between two parents of one mapping population (E Shu 3 Hao and Guang 2k-30) and eight accessions of cultivated sweetpotatoes. The results showed that 816 primer pairs could yield reproducible and strong amplification products, of which 195 (23.9%) and 342 (41.9%) primer pairs exhibited polymorphism between E Shu 3 Hao and Guang 2k-30 and among the 8 cultivated sweetpotatoes, respectively. Conclusion This study gives an insight into the frequency, type and distribution of sweetpotato EST-SSRs and demonstrates successful development of EST-SSR markers in cultivated sweetpotato. These EST-SSR markers could enrich the current resource of molecular markers for the sweetpotato community and would be useful for qualitative and quantitative trait mapping, marker-assisted selection, evolution and genetic diversity studies in cultivated sweetpotato and related Ipomoea species.

Abstract Background The tuberous root of sweetpotato is an important agricultural and biological organ. There are not sufficient transcriptomic and genomic data in public databases for understanding of the molecular mechanism underlying the tuberous root formation and development. Thus, high throughput transcriptome sequencing is needed to generate enormous transcript sequences from sweetpotato root for gene discovery and molecular marker development. Results In this study, more than 59 million sequencing reads were generated using Illumina paired-end sequencing technology. De novo assembly yielded 56,516 unigenes with an average length of 581 bp. Based on sequence similarity search with known proteins, a total of 35,051 (62.02%) genes were identified. Out of these annotated unigenes, 5,046 and 11,983 unigenes were assigned to gene ontology and clusters of orthologous group, respectively. Searching against the Kyoto Encyclopedia of Genes and Genomes Pathway database (KEGG) indicated that 17,598 (31.14%) unigenes were mapped to 124 KEGG pathways, and 11,056 were assigned to metabolic pathways, which were well represented by carbohydrate metabolism and biosynthesis of secondary metabolite. In addition, 4,114 cDNA SSRs (cSSRs) were identified as potential molecular markers in our unigenes. One hundred pairs of PCR primers were designed and used for validation of the amplification and assessment of the polymorphism in genomic DNA pools. The result revealed that 92 primer pairs were successfully amplified in initial screening tests. Conclusion This study generated a substantial fraction of sweetpotato transcript sequences, which can be used to discover novel genes associated with tuberous root formation and development and will also make it possible to construct high density microarrays for further characterization of gene expression profiles during these processes. Thousands of cSSR markers identified in the present study can enrich molecular markers and will facilitate marker-assisted selection in sweetpotato breeding. Overall, these sequences and markers will provide valuable resources for the sweetpotato community. Additionally, these results also suggested that transcriptome analysis based on Illumina paired-end sequencing is a powerful tool for gene discovery and molecular marker development for non-model species, especially those with large and complex genome.

CO2-enhanced oil recovery (CO2-EOR) is a crucial method for CO2 utilization and sequestration, representing an important zero-carbon or even negative-carbon emission reduction technology. However, the low viscosity of CO2 and reservoir heterogeneity often result in early gas breakthrough, significantly reducing CO2 utilization and sequestration efficiency. A water-alternating-gas (WAG) injection is a technique for mitigating gas breakthrough and viscous fingering in CO2-EOR. However, it encounters challenges related to insufficient mobility control in highly heterogeneous and fractured reservoirs, resulting in gas channeling and low sweep efficiency. Despite the extensive application and research of a WAG injection in oil and gas reservoirs, the most recent comprehensive review dates back to 2018, which focuses on the mechanisms of EOR using conventional WAG. Herein, we give an updated and comprehensive review to incorporate the latest advancements in CO2-WAG flooding techniques for enhanced sweep efficiency, which includes the theory, applications, fluid displacement mechanisms, and control strategies of a CO2-WAG injection. It addresses common challenges, operational issues, and remedial measures in WAG projects by covering studies from experiments, simulations, and pore-scale modeling. This review aims to provide guidance and serve as a reference for the application and research advancement of CO2-EOR techniques in heterogeneous and fractured reservoirs. [ABSTRACT FROM AUTHOR]

Despite breakthroughs in modern medical care, the incidence of cardiovascular disease (CVD) is even more prevalent globally. Increasing epidemiologic evidence indicates that emerging cardiovascular risk factors arising from the modern lifestyle, including psychosocial stress, sleep problems, unhealthy diet patterns, physical inactivity/sedentary behavior, alcohol consumption, and tobacco smoking, contribute significantly to this worldwide epidemic, while its underpinning mechanisms are enigmatic. Hematological and immune systems were recently demonstrated to play integrative roles in linking lifestyle to cardiovascular health. In particular, alterations in hematopoietic stem cell (HSC) homeostasis, which is usually characterized by proliferation, expansion, mobilization, megakaryocyte/myeloid-biased differentiation, and/or the pro-inflammatory priming of HSCs, have been shown to be involved in the persistent overproduction of pro-inflammatory myeloid leukocytes and platelets, the cellular protagonists of cardiovascular inflammation and thrombosis, respectively. Furthermore, certain lifestyle factors, such as a healthy diet pattern and physical exercise, have been documented to exert cardiovascular protective effects through promoting quiescence, bone marrow retention, balanced differentiation, and/or the anti-inflammatory priming of HSCs. Here, we review the current understanding of and progression in research on the mechanistic interrelationships among lifestyle, HSC homeostasis, and cardiovascular health. Given that adhering to a healthy lifestyle has become a mainstream primary preventative approach to lowering the cardiovascular burden, unmasking the causal links between lifestyle and cardiovascular health from the perspective of hematopoiesis would open new opportunities to prevent and treat CVD in the present age. [ABSTRACT FROM AUTHOR]

A physical simulation method with a combination of dynamic displacement and imbibition was established by integrating nuclear magnetic resonance (NMR) and CT scanning. The microscopic production mechanism of tight/shale oil in pore throat by dynamic imbibition and the influencing factors on the development effect of dynamic imbibition were analyzed. The dynamic seepage process of fracking-soaking-backflow-production integration was simulated, which reveals the dynamic production characteristics at different development stages and their contribution to enhancing oil recovery (EOR). The seepage of tight/shale reservoirs can be divided into three stages: strong displacement and weak imbibition as oil produced rapidly by displacement from macropores and fractures, weak displacement and strong imbibition as oil produced slowly by reverse imbibition from small pores, and weak displacement and weak imbibition at dynamic equilibrium. The greater displacement pressure results in the higher displacement recovery and the lower imbibition recovery. However, if the displacement pressure is too high, the injected water is easy to break through the front and reduce the recovery degree. The higher the permeability, the greater the imbibition and displacement recovery, the shorter the time of imbibition balance, and the higher the final recovery. The fractures can effectively increase the imbibition contact area between matrix and water, reduce the oil-water seepage resistance, promote the oil-water displacement between matrix and fracture, and improve the oil displacement rate and recovery of the matrix. The soaking after fracturing is beneficial to the imbibition replacement and energy storage of the fluid; also, the effective use of the carrying of the backflow fluid and the displacement in the mining stage is the key to enhancing oil recovery. [ABSTRACT FROM AUTHOR]