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Prostate cancer (PCa) is a frequently occurring malignancy in males, and epithelial mesenchymal transition (EMT) plays a critical role in PCa metastasis. Thus, developing biomarkers inhibiting EMT may provide significance for treatment of PCa. Hence, the aim of the current study was to investigate the mechanism by which FBP1 gene silencing influences PCa cell EMT, invasion and metastasis by mediating the MAPK pathway. PCa cell lines exhibiting the highest FBP1 expression were selected and treated with plasmids of siRNA-FBP1 sequence 1 and 2, pcDNA3.1-Flag-FBP1 (over-expression plasmid of FBP1), U0126 (an inhibitor of the ERK signaling pathway) and PD98059 (an inhibitor of the MEK signaling pathway). Cell proliferation, migration and invasion were detected by MTT assay, wound healing assay and Transwell assay, respectively. The mRNA and protein expression of related factors of EMT and MAPK signaling were determined by RT-qPCR and western blot analysis, respectively. Xenograft tumor growth after inoculation of DU145 cells was regularly analyzed in the nude mice. The positive expression of EMT markers was determined by immunohistochemistry. DU-145 and PC-3 cells displaying the highest FBP1 expression were selected for further analysis. The PCa cells treated with siRNA-FBP1 exhibited increased proliferation, migration rate and invasion, in addition to facilitated xenograft tumor growth. Notably, siRNA-FBP1 was identified to accelerate PCa cell EMT by elevating the expression of Vimentin and N-cadherin while diminishing E-cadherin expression via activation of the MAPK signaling pathway. The aforementioned results were reversed in PCa cells treated by pcDNA3.1-Flag-FBP1. Evidence has been provided in this study that FBP1 gene silencing activates the MAPK pathway, which ultimately promotes cell EMT, invasion and metastasis in PCa. [ABSTRACT FROM AUTHOR]

To establish a paradigm for the studies on the Environment Law is not only crucial for building the theoretical system of the Environmental Law but also for guiding us to solve the environmental problems. At present, the revolutionary paradigm of "the integration of the subject and the object" , strongly advocated by some scholars, has not only demonstrated their clear awareness of the necessity of paradigm construction but also enabled the theory of the Environmental Law to develop in a pluralistic fashion. With human existence in the order of values ignored to some extent and with a focus on theoretical construction at the cost of its feasibility, this paradigm, however, encounters a lot of difficulties especially when it comes to coping with the environmental problems in practice. Therefore, the paradigm change in the studies on the Environmental Law should make a shift to the practice-orientedness under the guidance of the principles of "people-orientedness" and "balanced development". [ABSTRACT FROM AUTHOR]

PURPOSE: To fabricate porous individual β-tricalcium phosphate scaffold and test its properties in dog. METHODS: A model of residual alveolar ridge in mandible of a dog was made and CT scanned after 3 months. The data of CT was transformed to 3-D format by MIMICS 7.0 and was made to resin model by rapid prototype technique. The residual alveolar ridge was reconstructed using silicon rubber, and its impression was made. Porous individual β-tricalcium phosphate scaffold and 5 samples were fabricated for precision and properties test. Porosity, water absorbing capacity and compressive strength of samples were tested with crystalling phase and pore structure were analysed by XRD and scanning electron microscope. RESULTS: We successfully fabricated a scaffold which fit the resin model well and consisted of β-TCP. Its porosity was 74%, water absorbing capacity was 48%, compressive strength was 4MPa, diameter of pore was 150 to 400µm, connecting diameter was 40µm. CONCLUSIONS: We can fabricate individual β-TCP scaffold which fit the model well by combination of traditional method and rapid prototype technique. Supported by Shanghai Leading Academic Discipline Project(Grant No.T0202). [ABSTRACT FROM AUTHOR]

Objective: To investigate the genotype distribution of thalassemia in the population of childbearing age in Yulin area., Methods: The polymerase reaction (PCR) combined with agargel eletrophoresis and reserve dot bolt hybridization was used to detected the α- and β-thalassemia gene in 31 769 cases of suspected thalassemia population at childbearing-age., Results: A total of 22 254 cases were identified as thalassemia gene detetion or mutation in 31 769 cases with a detecting rate of 70.05%, and the detecting rate of α-thalassemia, β-thalassemia and α-combining β-thalassemia were 45.86% (14 569/31 769), 19.45% (6 178/31 769) and 4.74% (1 507/31 769) respectively. 28 kinds of α-thalassemia gene mutations were detected, the common mutations were as follows: -- SEA /αα (28.18%), -α 3.7 /αα (6.29%), -α 4.2 /αα (3.66%), α CS α/αα (1.93%) and α WS α/αα (1.89%)，and including two rare gene mutations: - THAI and HKαα. 16 kinds of β-thalassemia gene mutations were detected, the common mutations were as follows: β 41-42 /β N (9.41%), β -28 /β N (3.05%), β -17 /β N (2.86%) and β 654 /β N (2.18%). 93 kinds of α combining β-thalassemia gene mutations were detected, the common mutations were as follows: -- SEA /αα (1.05%) and -α 3.7 /αα (0.56%) combining β 41-42 /βN., Conclusion: The detection rate of thalassemia gene is high in Yulin caildbearing-age population, and there is diversity in mutation spectrums of thalassemia. The most common genotypes are -- SEA /αα in α-thalassemia and β 41-42 /βN in β-thalassemia. The results are beneficial for the intervention and genetic consultation of thalassemia.

Purpose: To fabricate porous individual beta-tricalcium phosphate scaffold and test its properties in dog., Methods: A model of residual alveolar ridge in mandible of a dog was made and CT scanned after 3 months.The data of CT was transformed to 3-D format by MIMICS 7.0 and was made to resin model by rapid prototype technique.The residual alveolar ridge was reconstructed using silicon rubber, and its impression was made.Porous individual beta-tricalcium phosphate scaffold and 5 samples were fabricated for precision and properties test.Porosity, water absorbing capacity and compressive strength of samples were tested with crystalling phase and pore structure were analysed by XRD and scanning electron microscope., Results: We successfully fabricated a scaffold which fit the resin model well and consisted of beta-TCP. Its porosity was 74%,water absorbing capacity was 48%,compressive strength was 4 MPa,diameter of pore was 150 to 400 microm,connecting diameter was 40 microm., Conclusions: We can fabricate individual beta-TCP scaffold which fit the model well by combination of traditional method and rapid prototype technique.Supported by Shanghai Leading Academic Discipline Project(Grant No.T0202).

Objective: To evaluate the cytotoxicity of strontium substituted hydroxyapatite., Methods: Cell Relative Growth Rate(RGR) method, MTT assay and Flow Cytometry(FCM) method were used, and the strontium substituted hydroxyapatite contained different strontium concentration(0%,1%,5%,10%,100%)., Results: It was found that there's no apparent cytotoxicity of all the strontium substituted hydroxyapatite,but the cytotoxicity increased as the strontium concentration raised. As the FCM method appeared, there was no apparent difference between the pure hydroxyapatite and 1%,5% strontium substituted hydroxyapatite., Conclusion: Strontium substituted hydroxyapatite has good biocompatibility, and 10%,100% strontium substituted hydroxyapatite has weak cytotoxicity.