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13 results on '"CERPER Institut de Recherche Pierre Fabre"'

1. Boosting γδ T cell-mediated antibody-dependent cellular cytotoxicity by PD-1 blockade in follicular lymphoma

Author

Renaud Morin, Don-Marc Franchini, Loic Ysebaert, Christine Bezombes, Cédric Rossi, J. M. Lagarde, Jean-Jacques Fournié, Christine Jean, Ariel Savina, Julie Bordenave, Mary Poupot, Patricia Pérez-Galán, Alba Matas Céspedes, Camille Laurent, Pauline Gravelle, Marie Tosolini, Emilie Decaup, Christian Klein, Laetitia Ligat, Service d'Hématologie Clinique (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Institut Universitaire du Cancer de Toulouse - Oncopole (IUCT Oncopole - UMR 1037), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherches en Cancérologie de Toulouse (CRCT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre d'Immunologie de Marseille - Luminy (CIML), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), CERPER Institut de Recherche Pierre Fabre, Centre de Recherche Pierre Fabre (Centre de R&D Pierre Fabre), PIERRE FABRE-PIERRE FABRE, Immunité et cancer (U932), Université Paris Descartes - Paris 5 (UPD5)-Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Universitat de Barcelona (UB), Centre de Physiopathologie Toulouse Purpan (CPTP), Poupot, Mary, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale (INSERM), Immunologie et Cancérologie Intégrative, Centre de Recherche des Cordeliers (CRC), Université Paris Diderot - Paris 7 (UPD7)-École pratique des hautes études (EPHE)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Diderot - Paris 7 (UPD7)-École pratique des hautes études (EPHE)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Laboratoire Jacques-Louis Lions (LJLL), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Institut de recherche en informatique de Toulouse (IRIT), Université Toulouse 1 Capitole (UT1)-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées, Pierre Fabre Dermo-Cosmétique, Pierre Fabre Applied Skin Research Centre, Service d'Hématologie [IUCT Toulouse], Université Fédérale Toulouse Midi-Pyrénées-Institut Universitaire du Cancer de Toulouse - Oncopole (IUCT Oncopole - UMR 1037), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale (INSERM), Surfaces Cellulaires et Signalisation chez les Végétaux (SCSV), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Centre Régional de Lutte contre le cancer - Centre Georges-François Leclerc (CRLCC - CGFL), Institut de Mathématiques de Bourgogne [Dijon] (IMB), Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Centre de Physiopathologie Toulouse Purpan ex IFR 30 et IFR 150 (CPTP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Toulouse III - Paul Sabatier (UT3), Institut de Recherche Pierre Fabre, Université Paris Descartes - Paris 5 (UPD5)-Institut Curie-Institut National de la Santé et de la Recherche Médicale (INSERM), Immunologie et Cancérologie Intégratives (CRC - Inserm U1138), Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (UNICANCER/CRLCC-CGFL), UNICANCER, Université de Toulouse (UT)-Université de Toulouse (UT)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), and École pratique des hautes études (EPHE)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-École pratique des hautes études (EPHE)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)

Subjects
0301 basic medicine, lcsh:Immunologic diseases. Allergy, 3D model, [SDV.MHEP.HEM] Life Sciences [q-bio]/Human health and pathology/Hematology, [SDV.IMM] Life Sciences [q-bio]/Immunology, T cell, [SDV]Life Sciences [q-bio], Immunology, Cell, Follicular lymphoma, chemical and pharmacologic phenomena, [SDV.CAN]Life Sciences [q-bio]/Cancer, Biology, CD16, lcsh:RC254-282, γδ T cells, 03 medical and health sciences, 0302 clinical medicine, Immune system, follicular lymphoma, [SDV.CAN] Life Sciences [q-bio]/Cancer, PD-1, medicine, Immunology and Allergy, ComputingMilieux_MISCELLANEOUS, Original Research, Antibody-dependent cell-mediated cytotoxicity, [SDV.MHEP.HEM]Life Sciences [q-bio]/Human health and pathology/Hematology, medicine.disease, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, 3. Good health, Lymphoma, Cytolysis, 030104 developmental biology, medicine.anatomical_structure, Oncology, 030220 oncology & carcinogenesis, Cancer research, [SDV.IMM]Life Sciences [q-bio]/Immunology, anti-CD20 MAbs, lcsh:RC581-607
Abstract

International audience; Follicular lymphoma (FL) is a common non Hodgkin's lymphoma subtype in which immune escape mechanisms are implicated in resistance to chemo-immunotherapy. Although molecular studies point to qualitative and quantitative deregulation of immune checkpoints, in depth cellular analysis of FL immune escape is lacking. Here, by functional assays and in silico analyses we show that a subset of FL patients displays a 'high' immune escape phenotype. These FL cases are characterized by abundant infiltration of PD1+ CD16+ TCRVγ9Vδ2 γδ T lymphocytes. In a 3D co-culture assay (MALC), γδ T cells mediate both direct and indirect (ADCC in the presence of anti-CD20 mAbs) cytolytic activity against FL cell aggregates. Importantly, PD-1, which is expressed by most FL-infiltrating γδ T lymphocytes with ADCC capacity, impairs these functions. In conclusion, we identify a PD1-regulated γδ T cell cytolytic immune component in FL. Our data provide a treatment rational by PD-1 blockade aimed at boosting γδ T cell anti-tumor functions in FL.

Published
2018
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2. The use of suction blisters to measure sunscreen protection against UVR- induced DNA damage

Author

Emmanuel Questel, Eléonore Gravier, Gwendal Josse, Thierry Douki, Jimmy Le Digabel, CERPER Institut de Recherche Pierre Fabre, Centre de Recherche Pierre Fabre (Centre de R&D Pierre Fabre), PIERRE FABRE-PIERRE FABRE, Chimie Interface Biologie pour l’Environnement, la Santé et la Toxicologie (CIBEST ), SYstèmes Moléculaires et nanoMatériaux pour l’Energie et la Santé (SYMMES), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Laboratoire de Pharmacochimie Pierre Fabre Dermo-Cosmétique, Res Ctr, Institut Curie, Institut de Chimie du CNRS (INC)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Adult, Male, 0301 basic medicine, Suction (medicine), Ultraviolet Rays, DNA damage, education, Biophysics, Pyrimidine dimer, medicine.disease_cause, Young Adult, 030207 dermatology & venereal diseases, 03 medical and health sciences, Blister, 0302 clinical medicine, Tandem Mass Spectrometry, Healthy volunteers, medicine, Humans, Radiology, Nuclear Medicine and imaging, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Chromatography, High Pressure Liquid, ComputingMilieux_MISCELLANEOUS, Skin, Radiation, integumentary system, Radiological and Ultrasound Technology, Chemistry, food and beverages, Blisters, Molecular biology, Suction blister, Staining, 030104 developmental biology, Pyrimidine Dimers, Female, medicine.symptom, Sun Protection Factor, Sunscreening Agents, Genotoxicity, DNA Damage
Abstract

The formation of DNA photoproducts caused by solar UVR exposure needs to be investigated in-vivo and in particular in order to assess sunscreens' level of protection against solar genotoxicity. The study's purposes were: i) to evaluate if the roof of suction blisters is an appropriate sampling method for measuring photoproducts, and ii) to measure in-vivo sunscreen protection against cyclobutane pyrimidine dimers. Skin areas on the interior forearms of eight healthy volunteers were exposed in-vivo to 2 MED of simulated solar radiation (SSR) and to 15 MED on a sunscreen protected area. After irradiation, six suction blisters were induced and the blister roofs were collected. Analysis of SSR-induced CPDs was performed by two independent methods: a chromatography coupled to mass spectroscopy (HPLC-MS/MS) approach and a 3D-imaging of CPD immunostaining by multiphoton microscopy on floating epidermal sheets. HPLC-MS/MS analyses showed that SSR-unexposed skin presented no CPD dimers, whereas 2 MED SSR-exposed skin showed a significant number of TT-CPD. The sunscreen covered skin exposed to 15 MED appeared highly protected from DNA damage, as the amount of CPD-dimers remained below the detection limit. The multiphoton-immunostaining analysis consistently showed that no CPD staining was observed on the non-SSR-exposed skin. A significant increase of CPD staining intensity and number of CPD-positive cells were observed on the 2 MED SSR-exposed skin. Sunscreen protected skin presented a very low staining intensity and the number of CPD-positive cells remained very close to non-SSR-exposed skin. This study showed that suction blister samples are very appropriate for measuring CPD dimers in-vivo, and that sunscreens provide high protection against UVR-induced DNA damage.

Published
2018
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3. Better characterization of vinflunine pharmacokinetics variability and exposure/toxicity relationship to improve its use: analyses from 18 trials

Author

Schmitt , A., Nguyen , L, Zorza , G, Ferré , P., Pétain , A., Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (UNICANCER/CRLCC-CGFL), UNICANCER, Lipides - Nutrition - Cancer [Dijon - U1231] (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, CERPER Institut de Recherche Pierre Fabre, Centre de Recherche Pierre Fabre (Centre de R&D Pierre Fabre), PIERRE FABRE-PIERRE FABRE, Centre Régional de Lutte contre le cancer - Centre Georges-François Leclerc ( CRLCC - CGFL ), Lipides - Nutrition - Cancer [Dijon - U1231] ( LNC ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale ( INSERM ), and Institut de Recherche Pierre Fabre

Subjects
Neutropenia, Clinical Trials, Phase I as Topic, Dose-Response Relationship, Drug, PK/PD, [ SDV.SP.PHARMA ] Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology, Vinblastine, Antineoplastic Agents, Phytogenic, Models, Biological, modelling, Leukocyte Count, Clinical Trials, Phase II as Topic, Biological Variation, Population, [SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology, Humans, Administration, Intravenous, vinflunine, pharmacokinetics
Abstract

AIMS: Vinflunine is a novel tubulin‐targeted inhibitor indicated as a single agent for the treatment of bladder cancers after failure of prior platinum‐based therapy. Its pharmacokinetics (PK) and pharmacodynamics (PD) have been independently characterized through several phase I and phase II studies. However, no global pharmacometric analysis had been conducted as yet. METHODS: Vinflunine concentrations and safety data from 18 phase I and phase II studies were used to conduct population PK and PK/PD analysis, using Nonmem. A four‐compartment model was used to describe vinflunine PK and several covariates were tested to explain interindividual variability. In terms of PK/PD relationship, a semiphysiological population PK/PD model was applied to describe time course of absolute neutrophil counts (ANC) after vinflunine administration and logistic regression models were used to test the relationship between vinflunine exposure and toxicities. RESULTS: Vinflunine clearance is explained by creatinine clearance, body surface area and combination with PEGylated doxorubicin, leading to a decrease from 28.2 to 25.3% of the interindividual variability. When vinflunine dose is decreased, simulations of ANC time course (via a semiphysiological model) after vinflunine administration show a risk of neutropenia grade 3–4 at cycle 2 always lower than when dose is delayed. As an example, for moderate renal impaired patients, the risk is 42.1% when vinflunine is dosed at 320 mg m(–2) once every 4 weeks vs. 23.3% for 280 mg m(–2) once every 3 weeks. CONCLUSIONS: We propose for the first time a global comprehensive clinical pharmacological analysis for intravenous vinflunine that may help drive dose adjustment.

Published
2018
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4. A new experimental method for measuring skin's natural tension

Author

Gwendal Josse, Camille Garcin, Emmanuelle Jacquet, Fouad Khatyr, Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), CERPER Institut de Recherche Pierre Fabre, Centre de Recherche Pierre Fabre (Centre de R&D Pierre Fabre), and PIERRE FABRE-PIERRE FABRE

Subjects
medicine.medical_specialty, Materials science, Compressive Strength, medicine.medical_treatment, 0206 medical engineering, Posture, Human skin, 02 engineering and technology, Dermatology, Stress (mechanics), 030207 dermatology & venereal diseases, 03 medical and health sciences, 0302 clinical medicine, Skin Physiological Phenomena, medicine, Humans, ComputingMilieux_MISCELLANEOUS, integumentary system, Tension (physics), Equipment Design, Traction (orthopedics), [SPI.MECA]Engineering Sciences [physics]/Mechanics [physics.med-ph], Compression (physics), 020601 biomedical engineering, Finite element method, Surgery, Biomechanical Phenomena, Forearm, Elastomers, Stress, Mechanical, Biomedical engineering, Extensometer
Abstract

Purpose: The precise determination of skin's mechanical properties is still an open question. When performing an in vivo test, the piece of skin tested is not as well defined as it is in material testing. Moreover, the body zone and the body posture imply an initial stress on the skin. Consequently, a precise mechanical analysis needs a precise measurement of the natural skin tension. Methods: A new method and the relative device are presented. It is based on an extensiometry test. Skin is tested not only in traction but also in compression. The tested skin sample is well defined and protected from surrounding effects by follower tabs. The size and shape of the device have been optimised by a finite element modelisation. Results: The method was tested with elastomers pre-tensioned at different loads. It is shown that the initial tension can be retrieved with good precision. Tests were then performed in vivo on the forearm for different arm positions. It is shown that initial tension could be only clearly determined for the highest skin tension, although the skin presented very different traction behaviour with different arm positions. Conclusion: It is shown how body posture influences measurements. An innovative method for easily determining initial tension is presented. Nevertheless, further tests and device improvements are needed to apply this skin tension measurement for different body zones and body postures.

Published
2008

5. Analyse in vivo du comportement mécanique de la peau

Author

Delalleau, Alexandre, Josse, Gwendal, Lagarde, Jean-Michel, Zahouani, Hassan, Bergheau, Jean-Michel, Pierre Fabre Dermo-Cosmétique, Pierre Fabre Applied Skin Research Centre, CERPER Institut de Recherche Pierre Fabre, Centre de Recherche Pierre Fabre (Centre de R&D Pierre Fabre), PIERRE FABRE-PIERRE FABRE, Laboratoire de Tribologie et Dynamique des Systèmes (LTDS), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Ecole Nationale d'Ingénieurs de Saint Etienne-Centre National de la Recherche Scientifique (CNRS), and CSMA

Subjects
inverse method, suction, méthode inverse, peau, finite elements, éléments finis, viscoélasticité, succion, [PHYS.MECA]Physics [physics]/Mechanics [physics], viscoelasticity, Skin, extensométrie
Abstract

International audience; The mechanical properties of the skin are of potential interest in the identification of certain diseases, for assessing therapeutic intervention, or for predicting the effect of trauma. Due to its unusual structure, the skin presents a nonlinear viscoelastic anisotropic quasi incompressible mechanical behaviour which has to be studied in vivo. In this paper, a new method to identify the viscoelastic parameters of the human skin in vivo is presented. It lies in an inverse approach which compares experimental and finite element numerical results. Current works generally propose different approaches and hypotheses which consequently give results difficult to analyze. In this paper, two types of experimental tests are performed on the same subjects so as to compare the results obtained and to underline the advantages of the proposed method.; L'analyse des composantes mécaniques de la peau est d'une importance primordiale pour divers domaines tels la dermatologie, la chirurgie et la cosmétique. En raison de sa structure particulière, le tissu cutané présente un comportement complexe viscoélastique non linéaire anisotrope et quasi-incompressible, qui doit de surcroît être étudié de manière in vivo. Nous proposons dans cet article une méthode innovante d'analyse des caractéristiques viscoélastiques de la peau. Cette dernière repose sur une approche inverse du problème, couplant résultats expérimentaux et simulations numériques par éléments finis. Deux essais mécaniques effectués sur un même individu sont considérés. Une comparaison des résultats obtenus est ainsi possible afin de mener une discussion relative, d'une part aux phénomènes induits par chacun de ces tests, et, d'autre part, à l'originalité de l'approche.

Published
2007

6. Ultrasonic wave propagation and mechanical properties of human skin stretched in vivo

Author

Prevorovsky, Zdenek, Jacquet, Emmanuelle, Placet, Vincent, Josse, Gwendal, Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), CERPER Institut de Recherche Pierre Fabre, Centre de Recherche Pierre Fabre (Centre de R&D Pierre Fabre), and PIERRE FABRE-PIERRE FABRE

Subjects
[SPI.MECA]Engineering Sciences [physics]/Mechanics [physics.med-ph]
Abstract

December 9-13; International audience

Published
2007

7. About in vivo mechanical tests on human skin

Author

Khatyr, Fouad, Jacquet, Emmanuelle, Varchon, Daniel, Josse, Gwendal, Imberdis, Claude, Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), CERPER Institut de Recherche Pierre Fabre, Centre de Recherche Pierre Fabre (Centre de R&D Pierre Fabre), and PIERRE FABRE-PIERRE FABRE

Subjects
[PHYS.MECA.MEMA]Physics [physics]/Mechanics [physics]/Mechanics of materials [physics.class-ph], [SPI.MECA.MEMA]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Mechanics of materials [physics.class-ph], [SPI.MECA.BIOM]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph]
Published
2006

8. Modélisation du comportement viscoélastique de la peau in vivo

Author

Khatyr, Fouad, Imberdis, Claude, Varchon, Daniel, Josse, Gwendal, Lagarde, Jean-Michel, Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), CERPER Institut de Recherche Pierre Fabre, Centre de Recherche Pierre Fabre (Centre de R&D Pierre Fabre), and PIERRE FABRE-PIERRE FABRE

Subjects
[SPI.MECA.BIOM]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph]
Abstract

18-21 avril

Published
2005

9. Measure of the mechanical properties of skin with the test of suction. Comparison between 3 methods : geometric, Timoshenkos and finite elements

Author

Khatyr, Fouad, Imberdis, Claude, Varchon, Daniel, Lagarde, Jean-Michel, Josse, Gwendal, Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), CERPER Institut de Recherche Pierre Fabre, Centre de Recherche Pierre Fabre (Centre de R&D Pierre Fabre), and PIERRE FABRE-PIERRE FABRE

Subjects
[SPI.MECA.BIOM]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph]
Published
2005

10. Human keratinocytes acquire cellular cytotoxicity under UV-B irradiation. Implication of granzyme B and perforin.

Author

Hernandez-Pigeon H, Jean C, Charruyer A, Haure MJ, Titeux M, Tonasso L, Quillet-Mary A, Baudouin C, Charveron M, and Laurent G

Subjects
Cell Line, ErbB Receptors metabolism, Gene Expression Regulation radiation effects, Granzymes, Humans, MAP Kinase Signaling System physiology, Melanocytes, Membrane Glycoproteins genetics, Perforin, Pore Forming Cytotoxic Proteins, RNA, Messenger metabolism, Serine Endopeptidases genetics, T-Lymphocytes, Keratinocytes metabolism, Keratinocytes radiation effects, Membrane Glycoproteins metabolism, Serine Endopeptidases metabolism, Ultraviolet Rays
Abstract

Ultraviolet (UV) radiation from the sun is widely considered as a major cause of human skin photoaging and skin cancer. Granzyme B (GrB) and perforin (PFN) are two proteins contained in granules and implicated in one of the mechanisms by which cytotoxic lymphocytes and natural killer cells exert their cytotoxicity against virus-infected, alloreactive, or transformed cells. The distribution of GrB and PFN in the skin has received little attention. However, Berthou and co-workers (Berthou, C., Michel, L., Soulie, A., Jean-Louis, F., Flageul, B., Dubertret, L., Sigaux, F., Zhang, Y., and Sasportes, M. (1997) J. Immunol. 159, 5293-5300) described that, whereas freshly isolated epidermal cells did not express GrB or PFN, keratinocyte growth to confluence was associated with GrB and PFN mRNA and protein synthesis. In this work, we have investigated the possible role of UV-B on GrB and PFN expression in keratinocytes. We found that UV-B induces GrB and PFN expression in these cells through redox-, epidermal growth factor receptor-, and mitogen-activated protein kinase-dependent signaling. Furthermore, under UV irradiation, keratinocytes acquire a significant cytotoxicity, which is GrB and PFN dependent, toward a variety of cellular targets including transformed T-lymphocytes, melanocytes, and keratinocytes. This phenomenon may have important functional consequences in the regulation of skin inflammatory response and in the emergence of cancer skin.

Published
2006
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11. Topography and anisotropy of the skin surface with ageing.

Author

Lagarde JM, Rouvrais C, and Black D

Subjects
Adult, Age Factors, Aging pathology, Algorithms, Anisotropy, Female, Humans, Male, Middle Aged, Photography instrumentation, Sex Factors, Surface Properties, Aging physiology, Image Interpretation, Computer-Assisted methods, Imaging, Three-Dimensional methods, Photography methods, Skin anatomy & histology, Skin Physiological Phenomena, Tomography, Optical methods
Abstract

Background/purpose: Most studies of how the relief of the skin surface changes on ageing are based solely on topography, and a few have taken into account anisotropy parameters. However, the calculation of these parameters is often carried out manually and as the techniques of data acquisition have progressed rapidly, a certain degree of obsolescence has been reached. It is for this reason that we have undertaken a similar study but with more advanced equipment., Materials and Methods: The Dermatop system analyses the relief of Silflo negative prints of the skin surface. It is based on the projection of interference fringes and phase shift and provides surface parameters and not profiles. In addition, the Toposurf software enables image processing and the precise and automatic calculation of the topography parameters (roughness, developed surface area and peak-trough amplitude) and anisotropy (level of anisotropy and furrow density). These parameters were studied on the forearm and temple of 40 men and 40 women evenly distributed into two groups: the first including subjects aged 25-35 years and the second 50-65 years., Results: Roughness of both sites increases with age, independent of sex, but to a lesser extent in women than in men. The developed surface area and the peak-trough amplitude increase significantly with age irrespective of the site and the sex. The level of anisotropy increases with age, in both men and women, on the forearm and the temple, the site more exposed to light being more affected. The density of the furrows decreases with age in both sexes and both sites but with a greater increase for the temple, which is more exposed to solar ageing., Conclusion: The study of these new parameters will allow objective evaluation of the action of topical dermatological and cosmetic treatment, and new techniques in plastic surgery (e.g. laser resurfacing), and will enable the accurate follow-up of certain pathologies.

Published
2005
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12. Avena Rhealba inhibits A23187-stimulated arachidonic acid mobilization, eicosanoid release, and cPLA2 expression in human keratinocytes: potential in cutaneous inflammatory disorders.

Author

Aries MF, Vaissiere C, Pinelli E, Pipy B, and Charveron M

Subjects
Calcimycin pharmacology, Cell Line, Dermatitis drug therapy, Dose-Response Relationship, Drug, Humans, Ionophores pharmacology, Keratinocytes metabolism, Plant Extracts chemistry, Arachidonic Acid metabolism, Avena chemistry, Eicosanoids metabolism, Keratinocytes drug effects, Phospholipases A metabolism, Plant Extracts pharmacology
Abstract

The aim of the present study was to examine the effects of Avena Rhealba (AR) oatmeal extract on the metabolism of arachidonic acid (AA) and eicosanoids as well as on the expression of cytosolic phospholipase A(2 )(cPLA(2)) in the human keratinocyte cell line HaCaT. For this purpose, we examined the effects of AR on basal and A23187-triggered release of [(3)H]-AA from phospholipids and on the production of [(3)H]-labeled metabolites of the cyclooxygenase (CO) and 5-lipoxygenase (LO) pathways. AR was found to inhibit A23187-triggered [(3)H]-AA mobilization from phospholipids (p<0.05) and production of [(3)H]-labeled metabolites of CO (p<0.05) and LO (p<0.05) pathways. These results suggest AR decreases PLA(2)-dependent mobilization of AA from phospholipids. A closer examination of the effects of AR on prostaglandin 6KF1alpha (6KPGF1alpha), the stable metabolite of prostacyclin, revealed dose-dependent inhibition of this AA metabolite. AR also decreased A23187- and tumor necrosis factor alpha-induced cPLA(2) overexpression, as shown by cPLA(2) immunodetection and mRNA expression. These results demonstrate the high potential of AR in the treatment of inflammatory diseases of the skin.

Published
2005
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13. Environmental pollutants and skin cancer.

Author

Baudouin C, Charveron M, Tarroux R, and Gall Y

Subjects
Animals, Arsenic toxicity, Cadmium toxicity, Female, Humans, Lead toxicity, Male, Neoplasms, Radiation-Induced etiology, Ozone toxicity, Polycyclic Aromatic Hydrocarbons toxicity, Skin Neoplasms chemically induced, Ultraviolet Rays adverse effects, Environmental Pollutants toxicity, Skin Neoplasms etiology
Abstract

We are increasingly exposed to environmental pollution. Pollutants can be inhaled, ingested or come into contact with the skin depending on the form in which they occur. On metabolization, activation, or accumulation, pollutants can become extremely toxic for the vital organs and this is often related to a strong genotoxic effect. Since the skin acts as a barrier between the organism and the environment, it is frequently directly exposed to pollution. It is very often degraded by polluting agents and acts as an inlet toward other tissues. Numerous studies in man recognize and demonstrate the carcinogenic power of certain pollutants in the digestive and respiratory tracts. The "pollutants" that react most specifically with the skin are: ultraviolet radiation, polycyclic aromatic hydrocarbons (e.g., benzo[a]pyrene), volatile organic compounds (e.g., benzene), heavy metals, and ozone. Ultraviolet radiation, a "physical" pollutant, has been described as being the factor responsible for most skin cancers in man. The genotoxicity of UV light is well documented (type of lesion or mutation, etc.) and its carcinogenic effect is clearly demonstrated in vivo in man. A few epidemiological studies describe the carcinogenicity of certain pollutants such as arsenic or lead on the skin. However, most of the evidence for the role of pollutants in skin cancers comes from in vivo animal studies or from in vitro studies (e.g., PAHs). In this report, different studies are presented to illustrate the research strategies developed to investigate the mechanism of action of "chemical" pollutants and their potential role in human skin pathology. All the study models and the associated techniques of investigation are tools for a better understanding and thus more efficient prevention of the deleterious effects caused by the environment.

Published
2002
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