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2 results on '"CASEIN-DERIVED TRIPEPTIDES"'

1. Oral administration of the milk casein-derived tripeptide Val-Pro-Pro attenuates high-fat diet-induced adipose tissue inflammation in mice.

Author

Aihara, Kotaro, Osaka, Mizuko, and Yoshida, Masayuki

Subjects
RNA analysis, OBESITY complications, ADIPOSE tissues, ANALYSIS of variance, ANIMAL experimentation, CHRONIC diseases, FLOW cytometry, FAT content of food, INFLAMMATION, MICE, MILK proteins, PEPTIDES, POLYMERASE chain reaction, PROBABILITY theory, RESEARCH funding, T-test (Statistics), DATA analysis software, GENE expression profiling, DISEASE complications
Abstract

Inflammation of adipose tissue triggers the metabolic syndrome, atherosclerosis and CHD. In the present study, we investigated whether the milk casein-derived tripeptide valine-proline-proline (VPP) has an anti-inflammatory effect on the adipose tissue of high-fat diet (HFD)-fed mice. Male C57BL/6J mice (7 weeks of age) were fed ad libitum with either a HFD and plain tap water (HFD group) or a HFD and water containing 0·3 mg VPP/ml (HFD+VPP group) for 10 weeks. The results showed that the expression level of CD18 in the peripheral blood monocytes of the HFD+VPP group was significantly decreased compared with the level observed in those of the HFD group. Activated monocytes and pro-inflammatory macrophages were accumulated in the stromal vascular fractions of the adipose tissue from HFD-fed mice, which were significantly decreased in those supplemented with VPP. The formation of crown-like structures rich in pro-inflammatory macrophages was also significantly reduced in the adipose tissue of mice administered with VPP. Real-time PCR analysis revealed that the expression of monocyte chemoattractant protein-1 and that of the pro-inflammatory cytokine IL-6 in adipose tissue tend to be lower in the HFD+VPP group than in the HFD group. These observations indicate that oral administration of VPP exerts an anti-inflammatory effect on the adipose tissue of HFD-fed mice, which may eventually lead to the primary prevention of chronic inflammation-related diseases. [ABSTRACT FROM PUBLISHER]

Published
2014
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2. Bradykinin -induced vasodilatation: Role of age, ACE1-inhibitory peptide, mas- and bradykinin receptors

Author

Riitta Korpela, Heikki Vapaatalo, Aino Siltari, Medicum, Department of Pharmacology, and Riitta Anneli Korpela / Principal Investigator

Subjects
0301 basic medicine, Captopril, Receptor, Bradykinin B2, Physiology, Vasodilatation, BLOOD-PRESSURE, Vasodilation, Angiotensin-Converting Enzyme Inhibitors, Blood Pressure, 030204 cardiovascular system & hematology, Receptor, Bradykinin B1, Biochemistry, Proto-Oncogene Mas, Receptors, G-Protein-Coupled, chemistry.chemical_compound, MILK, 0302 clinical medicine, Endocrinology, Receptor, biology, Chemistry, CASEIN-DERIVED TRIPEPTIDES, Mesenteric Arteries, B-2 RECEPTORS, Ile-Pro-Pro, 317 Pharmacy, Hypertension, medicine.symptom, Oligopeptides, ANGIOTENSIN-CONVERTING ENZYME, medicine.medical_specialty, Bradykinin, 03 medical and health sciences, Cellular and Molecular Neuroscience, Internal medicine, Proto-Oncogene Proteins, Renin–angiotensin system, medicine, Animals, Humans, Bradykinin receptor, Rats, Wistar, SPONTANEOUSLY HYPERTENSIVE-RATS, ARACHIDONIC-ACID RELEASE, ACE-inhibition, Bioactive peptides, Antagonist, Angiotensin-converting enzyme, IN-VITRO, Vascular function, Peptide Fragments, Rats, Ageing, 030104 developmental biology, ENDOTHELIAL DYSFUNCTION, biology.protein, 3111 Biomedicine, Angiotensin I, VAL-PRO-PRO, Vasoconstriction
Abstract

Bradykinin exerts its vascular actions via two types of receptors, the non-constitutively expressed bradykinin receptor type 1 (BR1) and the constitutive type 2 receptor (BR2). Bradykinin-induced vasorelaxation is age-dependent, a phenomenon related to the varying amounts of BR1 and BR2 in the vasculature. Isoleucine-proline-proline (Ile-Pro-Pro), a bioactive tripeptide, lowers elevated blood pressure and improves impaired endothelium-dependent vasorelaxation in hypertensive rats. It inhibits angiotensin converting enzyme 1 (ACE1). Other mechanisms of action have also been postulated. The aims of the study were to clarify the underlying mechanisms of the age-dependency of bradykinin-induced vasodilatation such as the roles of the two bradykinin receptors, themas-receptor and synergism with Ile-Pro-Pro. The vascular response studies were conducted using mesenteric artery and aorta rings from normotensive 6 wk. (young) and 22 wk. (old) Wistar rats. Cumulative dosing of acetylcholine, bradykinin and angiotensin(1-7) (Ang(1-7))were tested in phenylephrine-induced vasoconstriction with or without 10 min pre-incubation with antagonists against BR1-, BR2- or mas-receptors,Ang(1-7) or ACE1-inhibitors captopril and Ile-Pro-Pro. The bradykinin-induced vasorelaxation in vitro was age-dependent and it was improved by pre incubation with Ile-Pro-Pro, especially in old rats with endothelial dysfunction. The mas-receptor antagonist, D-Pro7-Ang(1-7) abolished bradykinin-induced relaxation totally. Interestingly, BR1 and BR2 antagonists only slightly reduced bradykinin-induced vasorelaxation, as an evidence for the involvement of other mechanisms in addition to receptor activation. In conclusion, bradykinin-induced vasorelaxation was age -dependent and He-Pro-Pro improved it. Mas receptor antagonist abolished relaxation while bradykinin receptor antagonist only slightly reduced it, suggesting that bradykinin-induced vasorelaxation is regulated also by other mechanisms than the classical BR1/BR2 pathway. (C) 2016 Elsevier Inc: All rights reserved.

Published
2016

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