1. A rRNA-mRNA base pairing model for UGA-dependent termination
- Author
-
B. Kleuvers, C.D. Prescott, and H U Göringer
- Subjects
Genetics ,Models, Molecular ,Base Sequence ,Stereochemistry ,Translational termination ,Base pair ,Termination factor ,Molecular Sequence Data ,General Medicine ,Ribosomal RNA ,Biology ,Peptide Chain Termination, Translational ,Antiparallel (biochemistry) ,Biochemistry ,Models, Biological ,Stop codon ,Base change ,RNA, Ribosomal, 16S ,Mutation ,Escherichia coli ,Nucleic Acid Conformation ,RNA, Messenger ,Codon ,Protein secondary structure ,Peptide Termination Factors - Abstract
A series of site-directed mutations has been constructed in E coli 16S rRNA and shown to suppress UGA-dependent translational termination. With the exception of the C726 to G base change, all were constructed in helix 34. Characterization of these mutations is reviewed here and from these data and mRNA-rRNA base pairing model for the termination event is presented. The interaction functions via antiparallel base pairing between either 1 of the 2 UCA motifs in helix 34 and the complementary UGA stop codon on the message, thus forming a quasicontinuous A-type helical structure that is further stabilized by stacking enthalpy. Finally, rRNA motifs potentially required for UAA and UAG-dependent translational termination are discussed.
- Published
- 1991