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Abstrract: This study was conducted in order to evaluate the transmission of caprine lentivirus to sheep using different experimental groups. The first one (colostrum group) was formed by nine lambs receiving colostrum from goats positive for small ruminant lentiviruses (SRLV). The second group (milk group) was established by nine lambs that received milk of these goats. Third was a control group, consisting of lambs that suckled colostrum and milk of negative mothers. Another experimental group (contact group) was formed by eight adult sheep, confined with two naturally infected goats. The groups were monitored by immunoblotting (IB), enzyme-linked immunosorbent assay (ELISA), agar gel immunodiffusion (AGID) and nested polymerase chain reaction (nPCR). All lambs that suckled colostrum and milk of infected goats and six sheep of the contact group had positive results in the nPCR, although seroconversion was detected only in three of the exposed animals, with no clinical lentiviruses manifestation, in 720 days of observation. There was a close relationship between viral sequences obtained from infected animals and the prototype CAEV-Cork. Thus, it was concluded that SRLV can be transmitted from goats to sheep, however, the degree of adaptation of the virus strain to the host species probably interferes with the infection persistence and seroconversion rate.

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:44:18Z No. of bitstreams: 1 S1808-16572013000100006.pdf: 312790 bytes, checksum: a4983bc644810110d5aaa8972b61312b (MD5) Made available in DSpace on 2013-08-22T18:44:18Z (GMT). No. of bitstreams: 1 S1808-16572013000100006.pdf: 312790 bytes, checksum: a4983bc644810110d5aaa8972b61312b (MD5) Previous issue date: 2013-03-01 Made available in DSpace on 2013-09-30T19:32:19Z (GMT). No. of bitstreams: 2 S1808-16572013000100006.pdf: 312790 bytes, checksum: a4983bc644810110d5aaa8972b61312b (MD5) S1808-16572013000100006.pdf.txt: 34563 bytes, checksum: ed0b5b5bd3929b639ea95af8e23456be (MD5) Previous issue date: 2013-03-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:08:49Z No. of bitstreams: 2 S1808-16572013000100006.pdf: 312790 bytes, checksum: a4983bc644810110d5aaa8972b61312b (MD5) S1808-16572013000100006.pdf.txt: 34563 bytes, checksum: ed0b5b5bd3929b639ea95af8e23456be (MD5) Made available in DSpace on 2014-05-20T15:08:49Z (GMT). No. of bitstreams: 2 S1808-16572013000100006.pdf: 312790 bytes, checksum: a4983bc644810110d5aaa8972b61312b (MD5) S1808-16572013000100006.pdf.txt: 34563 bytes, checksum: ed0b5b5bd3929b639ea95af8e23456be (MD5) Previous issue date: 2013-03-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) O objetivo deste trabalho foi avaliar a ocorrência de ovinos soropositivos para o vírus da língua azul (VLA) no Estado do Ceará, Brasil, e analisar as proteínas imunogênicas das cepas virais circulantes nesses rebanhos. O teste de imunodifusão em gel de agarose (IDGA) foi utilizado para pesquisar 271 amostras de soro oriundas de 16 rebanhos. Os resultados demonstraram que 27,3% (74/271) das amostras analisadas apresentaram anticorpos contra o agente e 68,8% (11/16) das propriedades tiveram animais positivos. O immunoblotting (IB) foi utilizado para analisar as proteínas imunogênicas do VLA a partir dos soros de animais positivos no IDGA. Os soros demonstraram forte reação contra a proteína viral VP2. Para o VLA, das sete proteínas estruturais, a VP2 é a principal a estimular a resposta imune protetora. Concluiu-se que a soropositividade para a língua azul (LA) nos rebanhos ovinos estudados no Ceará é alta, apesar dos animais não apresentarem sinais clínicos, indicativo de que o vírus ocorra de forma endêmica. Além disso, a resistência à doença apresentada pelos animais pode estar relacionada com a forte reação imunológica desses à proteína VP2. Sendo assim, outros estudos são necessários para melhor esclarecer a situação epidemiológica da LA no país, através da identificação dos vetores e sorotipos virais circulantes nas diferentes regiões. The objective of this work was to verify the occurrence of sheep serologically positive for bluetongue virus (BTV) in the state of Ceará, Brazil, and analyze immunogenic proteins of circulating viral strains in these flocks. The agar gel immunodifusion test (AGID) was used to examine 271 serum samples from 16 herds. The results demonstrated that 27.3% (74/271) of the analyzed samples presented antibodies for the agent, and that 68.8% (11/16) of the properties presented positive animals. Immunoblotting (IB) was used to analyze the immunogenic proteins of BTV derived from AGID positive sera. Sera showed strong reaction against viral protein VP2. of the seven BTV structural proteins, VP2 is the major protein to elicit protective immune responses. It was concluded that bluetongue (BT) seropositivity in sheep flocks studied in Ceará is high, despite that the animal's do not show clinical signs, indicating that it occurs in an endemic form. The animals' resistance to the disease may be related to the strong immune response to the protein VP2. Therefore, further studies are needed to better clarify the epidemiological situation of BT in Brazilian sheep flocks, through the identification of viral vectors and serotypes circulating in different regions. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Caprinos e Ovinos Universidade Federal da Bahia (UFBA) Universidade Estadual do Ceará Faculdade de Veterinária Laboratório de Virologia Universidade Estadual do Vale do Acaraú Departamento de Biologia Universidade Federal do Recôncavo da Bahia Centro de Ciências Agrárias Universidade Estadual Paulista Universidade Estadual Paulista

Resumo: Com o objetivo de analisar a ocorrência de anticorpos contra Brucella ovis em ovinos no semiárido baiano, este inquérito foi conduzido em rebanhos da microrregião de Juazeiro, Bahia. O teste de imunodifusão em gel de ágar (IDGA) foi utilizado para examinar 694 amostras de soro de 58 rebanhos. Anticorpos para B. ovis foram observados em cinco (0,72%) dos animais investigados. Acredita-se que o baixo número de soropositivos deva-se às características dos sistemas de produção, com predominância do tipo de exploração extensiva de animais localmente adaptados, mestiços e sem raça definida para a produção de carne e pele, com baixa produtividade e tecnificação. [Sero-epidemiological survey for Brucella ovis infection in sheep flocks of Bahia state, Brazil]. Abstract: In order to analyze the occurrence of antibodies to Brucella ovis in sheep in a semi-arid region, this survey was conducted in the micro-region of Juazeiro, Bahia State, Brazil. The agar gel immunodifusion test (AGID) was used to examine 694 serum samples of 58 flocks. Antibodies to B. ovis were found in 5 (0.72%) of the investigated animals. It is believed that this low number of positive cases is related to the characteristics of the production. The predominant system is the extensive one, with presence of locally adapted breeds and crossbred animals, aiming at the production of meat and skin, with low productivity rates and technical development.

With the objective of detecting the presence of caprine lentivirus (CLV) in ewe milk and in ram semen, ten matrixes and four reproducers experimentally infected with CLV were used. Samples of ewe milk were collected during the four months of lactation, five collections per animal, totaling 50 samples. Regarding the rams, eight semen collections were made per animal, during one year of experimentation, totaling 32 samples. The milk and semen samples were submitted to DNA extraction and the nested polymerase chain reaction test (nPCR) to detect CLV proviral DNA. Eight (16%) of the milk samples were positive in nPCR originating from two ewes. Only one (3.12%) semen sample was positive. The amplification products were sequenced, and were confirmed to be a CLV genomic sequence. Thus, the presence of CLV proviral DNA in sheep milk and semen was demonstrated, confirming the feasibility of infection between species, and alerting to the risk of spreading infections. [Lentivírus caprino em leite e sêmen de ovinos]. Resumo: Com o objetivo de detectar a presença do lentivírus caprino (LVC) no leite de ovelhas e no sêmen de carneiros, utilizaram-se 10 matrizes e quatro reprodutores infectados experimentalmente com o LVC. Foram coletadas amostras de leite das ovelhas durante os quatro meses de lactação, ocorrendo cinco coletas por animal, totalizando 50 amostras. Quanto aos carneiros, realizaram-se oito coletas de sêmen por animal, durante um ano de experimentação, totalizando 32 amostras. As amostras de leite e de sêmen foram submetidas à extração de DNA e à prova de reação em cadeia da polimerase do tipo nested (nPCR) visando à detecção de DNA proviral do LVC. Oito (16%) amostras de leite foram positivas na nPCR oriundas de duas ovelhas. Apenas uma (3,12%) amostra de sêmen apresentou positividade. Produtos da amplificação foram sequenciados, confirmando-se tratar de sequência genômica do LVC. Dessa forma, demonstrou-se a presença do DNA proviral do LVC em leite e sêmen de ovinos, confirmando a viabilidade da infecção entre espécies e, assim, alertando sobre o risco de que a infecção seja disseminada. Made available in DSpace on 2017-09-24T00:12:27Z (GMT). No. of bitstreams: 1 CNPC2017Caprine.pdf: 376528 bytes, checksum: 3ae3a9af4fdd105cae76695fa5801913 (MD5) Previous issue date: 2017-09-22 [ALICE ANDRIOLI PINHEIRO].