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3. 212 - The Effect of Bulk Solvent Structure on the Temperature Dependence of the Reduction Potential of Cytochrome c

Author

C. William Anderson, Chin-Ho Su, William R. Heineman, George P. Kreishman, and H. Brian Halsall

Subjects
Aqueous solution, Cytochrome, biology, Chemistry, Cytochrome c, Sodium, Inorganic chemistry, Biophysics, Halide, chemistry.chemical_element, Atmospheric temperature range, Chloride, Electrochemistry, medicine, biology.protein, Physical and Theoretical Chemistry, Anion binding, medicine.drug
Abstract

The reduction potential of horse heart cytochrome c in various sodium halide solutions in H2O and D2O has been measured over the temperature range of 25 to 50 °C. In aqueous chloride solutions the temperature dependence was biphasic with the intersection point at 42 °C. This biphasic behavior is interpreted in terms of chloride-induced bulk destructuring of water at 42 °C. All samples in D2O and samples in H2O not containing chloride ion gave a linear temperature dependence. The decrease in reduction potential with increasing temperature for solutions containing F−, Cl−, Br− and I − correlates with the extent of anion binding to the oxidized form of cytochrome c.

Published
1978
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4. A small-volume thin-layer spectroelectrochemical cell for the study of biological components

Author

H. Brian Halsall, William R. Heineman, and C. William Anderson

Subjects
Working electrode, Materials science, business.industry, Small volume, Thin layer, Biophysics, Analytical chemistry, Cytochrome c Group, Cell Biology, Electrochemistry, Biochemistry, Redox, Characterization (materials science), Coulometry, Spectrophotometry, Optoelectronics, Gold, Cyclic voltammetry, business, Electrodes, Molecular Biology
Abstract

A small-volume (∼60 μl) spectroelectrochemical cell was constructed and characterized.The working electrode is An minigrid, and the spectral window is from 170 nm to 2 μm. The solution is held in a thin layer around the working electrode, providing the advantages of thin-layer electrochemistry. The cell can be constructed from common materials in about 6-h working time. The cell also has flow-through and vacuum capabilities, with only minor modifications. Characterization of the cell was done by cyclic voltammetry and coulometry on ferri-ferrocyanide and spectroelectrochemistry on cytochrome c and o-tolidine. The optical and electrochemical windows of this cell, as well as its convenient and versatile operation, make it useful for the study of a wide variety of biological redox components.

Published
1979
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5. Cadmium(II)-Exchanged Zeolite as a Solid Sorbent for the Preconcentration and Determination of Atmospheric Hydrogen Sulfide II: Spectroscopic Techniques

Author

C. William Anderson, Sivasankararao. Vasireddy, Kenneth W. Street, Rebecca A. LaRue-Filio, Stephen J. Simon, Harry B. Mark, and Michael P. Fuller

Subjects
chemistry.chemical_classification, Cadmium, Sorbent, medicine.diagnostic_test, Sulfide, Hydrogen sulfide, 010401 analytical chemistry, Inorganic chemistry, chemistry.chemical_element, Infrared spectroscopy, 01 natural sciences, 0104 chemical sciences, 010309 optics, chemistry.chemical_compound, Ultraviolet visible spectroscopy, chemistry, Spectrophotometry, 0103 physical sciences, medicine, Instrumentation, Spectroscopy, Methylene blue
Abstract

Instrumental techniques are compared for usage in the determination of atmospheric H2S which has been preconcentrated on Cd(II)-exchanged zeolite. The study covers the range of 0.01 to 100 μg of immobilized sulfide. For high concentrations (2–100 μg), combustion analysis followed by nondispersive IR is the simplest and most precise technique, and results with the use of this method are accurate to ±3% relative. In the range of 0.25–40 μg, the sulfide, after conversion to methylene blue, may be determined by conventional spectrophotometry, with a relative precision of ±13%. For the lowest concentration range investigated (0.01–2.0 μg), photoacoustic spectroscopy gave the only quantitative results after the H2S was converted to methylene blue.

Published
1985
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7. The temperature dependence of the redox potential of horse heart cytochrome c in sodium chloride solutions

Author

William R. Heineman, H. Brian Halsall, C. William Anderson, and George P. Kreishman

Subjects
Sodium, Inorganic chemistry, Biophysics, chemistry.chemical_element, Cytochrome c Group, Sodium Chloride, Biochemistry, Redox, chemistry.chemical_compound, Animals, Horses, Molecular Biology, biology, Cytochrome c, Myocardium, Temperature, Horse, Cell Biology, Atmospheric temperature range, Phosphate, Single line, Deuterium, chemistry, biology.protein, Potentiometry, Oxidation-Reduction, Protein Binding
Abstract

The E0′ values for the conversion of horse heart cytochrome c from the oxidized to the reduced form as a function of temperature have been measured in 0.10 M NaCl, 0.10 M sodium phosphate, pH 7.0 solutions in H2O and D2O. In H2O, the decrease in the E0′ value is linear with increasing temperature up to 42°C. Above this temperature, the decrease is again linear but with a much greater slope. In D2O solutions, however, this biphasic behavior was not observed but instead a single line was obtained over the temperature range studied (25°C to 50°C). These results are interpreted in terms of the ability of NaCl to cause a destructuring of the bulk H2O above 42°C but not in the more stable D2O (Kreishman, Foss, Inoue and Leifer (1976) Biochemistry, 15, 5431–5435). This decrease in water structure results in a shift in the equilibrium to the larger oxidized form as indicated by the decrease in E0′.

Published
1976

9. Applied Solar Energy and Introduction to Solar Technology and Solar Heating Systems: Analysis and Design with the Sun-Pulse Method

Author

David Kut, H. C. William Anderson, Gerald Hare, John R. Howell, Marian Jacobs Fisk, and Gordon F. Tully

Subjects
Materials science, Renewable Energy, Sustainability and the Environment, business.industry, Photovoltaic system, Energy Engineering and Power Technology, Solar energy, Engineering physics, Solar mirror, Photovoltaic thermal hybrid solar collector, Solar air conditioning, Photovoltaics, Passive solar building design, Solar cable, business
Published
1982
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10. Electrochemical and Spectrochemical Studies of Biological Redox Components

Author

KARL M. KADISH, WILLIAM R. HEINEMAN, C. WILLIAM ANDERSON, H. BRIAN HALSALL, MARILYN M. HURST, JAY M. JOHNSON, GEORGE P. KREISHMAN, BARBARA J. NORRIS, MICHAEL J. SIMONE, CHIH-HO SU, ERIC E. BANCROFT, HENRY N. BLOUNT, FRED M. HAWKRIDGE, VERNON T. TANIGUCHI, WALTHER R. ELLIS, VINCE CAMMARATA, JOHN WEBB, FRED C. ANSON, HARRY B. GRAY, RONALD L. BIRKE, JOHN R. LOMBARDI, LUIS A. SANCHEZ, J. P. GISSELBRECHT, M. GROSS, ROBERT R. GAGNÉ, ROBERT P. KREH, JOHN DODGE, EDMOND F. BOWDEN, M. J. EDDOWES, H. A. O. HILL, KATSUMI NIKI, TATSUHIKO YAGI, HIROO INOKUCHI, F. SCHELLER, G. STRNAD, A. B. P. LEVER, S. LICOCCIA, K. MAGNELL, P. C. MINOR, B. S. RAMASWAMY, ANDREAS BOTULINSKI, JOHANN WALTER BUCHLER, KIONG LAM LAY, JÜRGEN ENSLING, HANS TWILFER, JOCHEN BILLECKE, HEIKO LEUKEN, BERNHARD TONN, LAWRENCE A. BOTTOMLEY, LARRY OLSON, KAREY L. HOLLAND, JOSEPH JORDAN, CHRISTOPHER A. REED, HAROLD M. GOFF, MARTIN A. PHILLIPPI, ARDEN D. BOERSMA, ANDREW P. HANSEN, F. ANN WALKER, JUDITH A. BARRY, VIRGINIA L. BALKE, GREGORY A. McDERMOTT, MICHAEL Z. WU, PETER F. LINDE, BRIAN R. COLEM, KARL M. KADISH, WILLIAM R. HEINEMAN, C. WILLIAM ANDERSON, H. BRIAN HALSALL, MARILYN M. HURST, JAY M. JOHNSON, GEORGE P. KREISHMAN, BARBARA J. NORRIS, MICHAEL J. SIMONE, CHIH-HO SU, ERIC E. BANCROFT, HENRY N. BLOUNT, FRED M. HAWKRIDGE, VERNON T. TANIGUCHI, WALTHER R. ELLIS, VINCE CAMMARATA, JOHN WEBB, FRED C. ANSON, HARRY B. GRAY, RONALD L. BIRKE, JOHN R. LOMBARDI, LUIS A. SANCHEZ, J. P. GISSELBRECHT, M. GROSS, ROBERT R. GAGNÉ, ROBERT P. KREH, JOHN DODGE, EDMOND F. BOWDEN, M. J. EDDOWES, H. A. O. HILL, KATSUMI NIKI, TATSUHIKO YAGI, HIROO INOKUCHI, F. SCHELLER, G. STRNAD, A. B. P. LEVER, S. LICOCCIA, K. MAGNELL, P. C. MINOR, B. S. RAMASWAMY, ANDREAS BOTULINSKI, JOHANN WALTER BUCHLER, KIONG LAM LAY, JÜRGEN ENSLING, HANS TWILFER, JOCHEN BILLECKE, HEIKO LEUKEN, BERNHARD TONN, LAWRENCE A. BOTTOMLEY, LARRY OLSON, KAREY L. HOLLAND, JOSEPH JORDAN, CHRISTOPHER A. REED, HAROLD M. GOFF, MARTIN A. PHILLIPPI, ARDEN D. BOERSMA, ANDREW P. HANSEN, F. ANN WALKER, JUDITH A. BARRY, VIRGINIA L. BALKE, GREGORY A. McDERMOTT, MICHAEL Z. WU, PETER F. LINDE, and BRIAN R. COLEM

Subjects
Bioelectrochemistry--Congresses, Oxidation-reduction reaction--Congresses, Spectrum analysis--Congresses
Published
1982

11. Bioelectrochemistry: Ions, Surfaces, Membranes

Author

MARTIN BLANK, SHU CHIEN, L. J. LIS, R. P. RAND, V. A. PARSEGIAN, ARTHUR L. Y. LAU, ALAN C. McLAUGHLIN, ROBERT C. MacDONALD, STUART G. A. McLAUGHLIN, GIUSEPPE COLACICCO, MUKUL K. BASU, FRANCINE A. TANSEY, SHLOMO NIR, JOE BENTZ, ARCHIE R. PORTIS, C. J. VAN OSS, A. W. NEUMANN, R. J. GOOD, D. R. ABSOLOM, M.-F. LECOMPTE, I. R. MILLER, T. A. PAYENS, PAULA BOTH, K. M. JAN, SUNG HYE YIM, GERSON KEGELES, JOHN R. CANN, GEORGE P. KREISHMAN, CHIH-HO SU, C. WILLIAM ANDERSON, H. BRIAN HALSALL, WILLIAM R. HEINEMAN, CAROL J. DEUTSCH, ANDRIJ HOLIAN, DAVID F. WILSON, FELIX T. HONG, J. A. DESIMONE, G. L. HECK, S. K. DESIMONE, WILLIAM D. NIEMI, PHILIP C. SU, M. B. ABRAMSON, M. CHOI, E. SCHOFFENIELS, L. SOO, R. E. ABBOTT, V. S. VAIDHYANATHAN, ARTHUR A. PILLA, W. R. ADEY, S. M. BAWIN, S. J. SOCOLAR, Y. KANNO, W. R. LOEWENSTEIN, B. ROSE, I. SIMPSON, MAX BENDER, K. HILLMAN, L. TIRRI, M. GRABEN, D. FENSTER, M. ROSENSAFT, R. D. KULKARNI, E. D. GODDARD, G. S. MARGULES, S. B. DOTY, A. A. PILLA, F. M. ETZLER, W. DROST-HANSEN, MARTIN BLANK, SHU CHIEN, L. J. LIS, R. P. RAND, V. A. PARSEGIAN, ARTHUR L. Y. LAU, ALAN C. McLAUGHLIN, ROBERT C. MacDONALD, STUART G. A. McLAUGHLIN, GIUSEPPE COLACICCO, MUKUL K. BASU, FRANCINE A. TANSEY, SHLOMO NIR, JOE BENTZ, ARCHIE R. PORTIS, C. J. VAN OSS, A. W. NEUMANN, R. J. GOOD, D. R. ABSOLOM, M.-F. LECOMPTE, I. R. MILLER, T. A. PAYENS, PAULA BOTH, K. M. JAN, SUNG HYE YIM, GERSON KEGELES, JOHN R. CANN, GEORGE P. KREISHMAN, CHIH-HO SU, C. WILLIAM ANDERSON, H. BRIAN HALSALL, WILLIAM R. HEINEMAN, CAROL J. DEUTSCH, ANDRIJ HOLIAN, DAVID F. WILSON, FELIX T. HONG, J. A. DESIMONE, G. L. HECK, S. K. DESIMONE, WILLIAM D. NIEMI, PHILIP C. SU, M. B. ABRAMSON, M. CHOI, E. SCHOFFENIELS, L. SOO, R. E. ABBOTT, V. S. VAIDHYANATHAN, ARTHUR A. PILLA, W. R. ADEY, S. M. BAWIN, S. J. SOCOLAR, Y. KANNO, W. R. LOEWENSTEIN, B. ROSE, I. SIMPSON, MAX BENDER, K. HILLMAN, L. TIRRI, M. GRABEN, D. FENSTER, M. ROSENSAFT, R. D. KULKARNI, E. D. GODDARD, G. S. MARGULES, S. B. DOTY, A. A. PILLA, F. M. ETZLER, and W. DROST-HANSEN

Published
1980

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