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1. Leishmania infantum Nicolle, 1908 from southern Spain: Characterisation of the strains from human visceral and cutaneous leishmaniasis and from sandflies; with a numerical analysis of the isoenzymatic data

Author

J. M. Salinas Martinez de Lecea, F. Morillas Márquez, F. Ruiz Martinez, C. Sanchez Rabasco, M. C. Sanchiz Marin, J. Martín Sánchez, V. Delgado Florencio, and C. Acedo Sanchez

Subjects
Veterinary medicine, Visceral leishmaniasis, Cutaneous leishmaniasis, biology, Animal ecology, medicine, Parasitology, Leishmaniasis, Isoenzyme electrophoresis, Leishmania infantum, medicine.disease, biology.organism_classification
Abstract

This study presents the results of the characterisation of 17 strains ofLeishmania by isoenzyme electrophoresis from a focus of leishmaniasis in southern Spain: two from human visceral leishmaniasis, four from human cutaneous leishmaniasis and 11 from sandflies. The 17 strains are grouped in 6 zymodemes characterised by their variability as regards to the electrophoretic mobility of the enzymes MDH, G6PD, NP and ME. Thus, we confirm the high intraspecific variability ofLeishmania (L.) infantum in a focus of southern Spain, as already suggested by previous studies. Zymodemes GR-15 and GR-17 are also described for the first time in Spain, and they characteristically possess the same relative electrophoretic mobility in the enzyme ME (93). Sixteen zymodemes of theL. infantum complex found in southern Spain were numerically analysed on the basis of the enzymatic profiles of 122Leishmania strains characterised from this area.

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