34 results on '"C. Mendola"'
Search Results
2. High-dose rocuronium for rapid-sequence induction and reversal with sugammadex in two myasthenic patients
- Author
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P. Casarotti, C. Mendola, Gianmaria Cammarota, and F. Della Corte
- Subjects
medicine.medical_specialty ,medicine.drug_class ,business.industry ,medicine.medical_treatment ,Perforation (oil well) ,Muscle relaxant ,General Medicine ,Rapid sequence induction ,medicine.disease ,Neuromuscular monitoring ,Sugammadex ,Surgery ,Anesthesiology and Pain Medicine ,Anesthesia ,medicine ,Upper gastrointestinal bleeding ,Rocuronium ,Elective surgery ,business ,medicine.drug - Abstract
The anesthetic management of patients affected by myasthenia gravis is usually challenging in elective surgery and even more so in emergency procedures. The difficulties involved are several-fold, ranging from the choice of an appropriate muscle relaxant (i.e. one that enables safe and rapid airway management) to neuromuscular monitoring and normal muscular recovery. Additionally, optimizing patient conditions - either pharmacologically or with plasmapheresis - before intervention is well beyond the realm of possibility. We discuss the anesthetic management of two myasthenic patients undergoing emergency surgery (for sigmoid perforation and upper gastrointestinal bleeding respectively). In both cases, we opted for rapid-sequence induction with high-dose rocuronium to prevent inhalation of gastric contents. We also report on the implication of neuromuscular monitoring. We found that the rocuronium-sugammadex combination was a useful and effective option in the emergency setting.
- Published
- 2014
3. Reversal of rocuronium induced neuromuscular block with sugammadex or neostigmine: a large observational study
- Author
-
C Pagan De Paganis, C Mendola, Livia Pompei, S Manstretta, Paolo Murabito, S Tesoro, G. Della Rocca, L Fuggiano, A Gratarola, P Boninsegni, L Zamidei, P. Di Marco, and Augusto Tempia
- Subjects
medicine.medical_specialty ,Dose ,biology ,business.industry ,General Medicine ,Neuromuscular monitoring ,biology.organism_classification ,Sugammadex ,Neostigmine ,Pacu ,Surgery ,Anesthesiology and Pain Medicine ,Anesthesia ,medicine ,Rocuronium ,Anesthesia Recovery Period ,business ,Prospective cohort study ,medicine.drug - Abstract
Background This ‘real-life’ study aimed to analyze the time from the start of neostigmine or sugammadex administration to recovery to a train of four ratio (TOFr) of 0.9 in a real-life in patients receiving rocuronium. The secondary aims were to assess the proportion of patients: presenting TOFr
- Published
- 2013
4. Thoracic epidural analgesia in post-thoracotomy patients: comparison of three different concentrations of levobupivacaine and sufentanil
- Author
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C. Mendola, Daniela Ferrante, F. Della Corte, E. Oldani, Gianmaria Cammarota, G. Cecci, and Rosanna Vaschetto
- Subjects
Adult ,Male ,medicine.medical_specialty ,Sufentanil ,Nausea ,medicine.drug_class ,Sedation ,medicine.medical_treatment ,Analgesic ,Drug Administration Schedule ,Double-Blind Method ,medicine ,Humans ,Prospective Studies ,Thoracotomy ,Anesthetics, Local ,Aged ,Levobupivacaine ,Pain Measurement ,Aged, 80 and over ,Pain, Postoperative ,business.industry ,Local anesthetic ,Pruritus ,Middle Aged ,Bupivacaine ,Surgery ,Analgesia, Epidural ,Analgesics, Opioid ,Anesthesiology and Pain Medicine ,Anesthesia ,Postoperative Nausea and Vomiting ,Vomiting ,Female ,Hypotension ,medicine.symptom ,business ,medicine.drug - Abstract
Background Relative effects of dosage, volume and concentration of local anaesthetics used for postoperative thoracic epidural analgesia are still under debate. In this randomized, prospective, double-blinded study, we evaluated the incidence of side-effects such as changes in arterial pressure, postoperative nausea, vomiting, and pruritus in patients admitted for thoracic surgery during continuous thoracic epidural infusion using levobupivacaine and sufentanil mixture in three different volumes. Methods We studied 150 patients who underwent thoracotomy with a thoracic epidural catheter placed between T4 and T7. The patients were randomized into three groups which received 10 mg h−1 of levobupivacaine at three different concentrations (0.5%, 0.25%, and 0.15%), in combination with sufentanil at 2.6 μg h−1. Haemodynamic effects, pruritus, nausea, vomiting, sensory and motor block, pain score, additional analgesic requirement, sedation, and patient satisfaction were registered immediately after the surgical operation and on the first, second, and third postoperative days. Results We did not detect any differences in the incidence of side-effects such as changes in arterial pressure, and also postoperative nausea, vomiting, and pruritus. The three groups were also similar with regard to patient characteristics, sensory and motor block, pain score, analgesic rescue dose, sedation, and patient satisfaction. Conclusions The same dose of a mixture of levobupivacaine and sufentanil administered in three different volumes and concentrations during continuous thoracic epidural infusion for thoracotomy provided an equal incidence of adverse haemodynamic effects, nausea, vomiting, or pruritus.
- Published
- 2009
5. Cerebral cavernomas and seizures: a retrospective study on 163 patients who underwent pure lesionectomy
- Author
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Marina Casazza, Paolo Ferroli, Angelo Franzini, C. Mendola, C. Marras, and Giovanni Broggi
- Subjects
Adult ,Male ,medicine.medical_specialty ,Microsurgery ,Neurology ,Adolescent ,Dermatology ,Neurosurgical Procedures ,Hemangioma ,Angioma ,Epilepsy ,Seizures ,medicine ,Humans ,Neuroradiology ,Retrospective Studies ,business.industry ,Brain Neoplasms ,Retrospective cohort study ,General Medicine ,Middle Aged ,medicine.disease ,Magnetic Resonance Imaging ,Surgery ,Psychiatry and Mental health ,Image-guided surgery ,Hemangioma, Cavernous ,Treatment Outcome ,Female ,Neurology (clinical) ,Neurosurgery ,business - Abstract
The objective was to evaluate the outcome of microsurgical "pure" lesionectomy in patients with supratentorial cavernous angiomas presenting with seizures. For this retrospective study 163 patients with cavernoma-related epileptic seizures were selected. They all underwent surgery in a single institution between 1988 and 2003. A microsurgical frame/frameless guided minimally invasive transulcal "pure" lesionectomy was performed. The haemosiderin stained gliotic brain parenchyma that was usually found surrounding the lesion was not removed. Among the 99 patients with epilepsy and longer clinical history, 68 (68.7%) were found completely to be seizure-free, 10 (10.1%) presented sporadic and less frequent seizures and 17 (17.1%) remained unchanged. Sixty-three out of 64 (98.4%) patients who experienced only single or sporadic seizures were found to be completely seizure-free after surgery. Five patients were lost at follow-up (mean 48 months, range 0.5-14 years). Long-term morbidity was 1.8%. Mortality was null. No haemorrhagic episodes were observed during follow-up. Pure lesionectomy prevents bleeding and development of epilepsy in patients that receive early surgery after the epileptic onset. In most of the epileptic patients with a good concordance between the electroclinical data and the location of the angioma, good results can be achieved by this kind of surgery so that more invasive and costly studies to find and remove the epileptogenic cerebral parenchyma seem justified only after lesionectomy fails.
- Published
- 2005
6. Craniotomies without burr holes using an oscillating saw
- Author
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C. Mendola, Giulio Cantù, Francesco DiMeco, Khan W. Li, and Carlo L. Solero
- Subjects
Skull Base ,medicine.medical_specialty ,Gigli saw ,education.field_of_study ,business.industry ,Brain Neoplasms ,medicine.medical_treatment ,Burr holes ,Craniotome ,Surgical Instruments ,Skull Base Neoplasms ,Surgery ,Skull ,Fixation (surgical) ,medicine.anatomical_structure ,Dural venous sinuses ,medicine ,Humans ,Neurology (clinical) ,Craniofacial ,education ,business ,Craniotomy - Abstract
Objective. The object of this study is to describe a new method for performing craniotomies which obviates the need for burr holes, improves bony reconstruction, and reduces post-operative cosmetic deformities. Moreover, this technique provides excellent exposure of skull base structures and dural venous sinuses. Methods. Craniotomies in varied locations are created with the use of a micro-oscillating saw and chisel. No burr holes are used and reconstruction with plates and screws is unnecessary. Results. We initially applied our technique to approaches to the anterior skull base with combined craniofacial tumour resections. We have since performed over 2000 craniotomies of any size and shape in all supratentorial locations using the oscillating saw. Conclusions. We have found that our method creates better cosmetic results than standard techniques and is safer for craniotomies spanning dural venous sinuses. With experience, operating time was significantly reduced and costs were lowered because reconstruction with fixation devices was not needed.
- Published
- 2004
7. Post Thoracotomy Pain Syndrome
- Author
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F. Della Corte, C. Mendola, A. Messina, G. Cammarota, F. Della Corte, C. Mendola, A. Messina, and G. Cammarota
- Published
- 2012
- Full Text
- View/download PDF
8. Total intravenous anesthesia with propofol and remifentanil for elective non-cardiac surgery
- Author
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G, Pelosi, A, Gratarola, C, Pissaia, C, Mendola, and G, Bellomo
- Subjects
Male ,Remifentanil ,Piperidines ,Elective Surgical Procedures ,Anesthesia, Intravenous ,Humans ,Female ,Middle Aged ,Propofol ,Anesthetics, Combined ,Anesthetics, Intravenous - Abstract
Remifentanil is a highly effective mu opioid agonist with predictable pharmacokinetics and a close concentration-effect relationship. Moreover, studies on anesthetic drugs interactions show that optimal propofol concentrations decrease more significantly with remifentanil as compared with other opioids and recovery appears to be much faster than when propofol is combined with other opioids combinations. This intervention study was designed to evaluate the efficacy of propofol combined with remifentanil in elective non cardiac inpatient surgery.N. 405 patients undergoing intraperitoneal, head-neck, intrathoracic, major orthopaedics, breast and major vascular surgery received: remifentanil (1 microgram.kg-1 at induction; 0.50 microgram.kg-1.min-1 at laryngoscopy; 0.25 microgram.kg-1.min-1 at skin incision; 0.25-0.30 microgram.kg-1.min1 from skin incision to end of skin suture) and propofol (0.5-1 mg.kg-1 at induction; 5 mg.kg-1.h-1 at laryngoscopy; 5 mg.kg-1.h-1 at skin incision and 5 mg.kg1.h1 thereafter). Intraoperative end-points included somatic responses, tachycardia and hypertension to laryngoscopy and surgery. Incidence of intraoperative bradycardia, hypotension and muscle rigidity were also recorded. Postoperative end-points included Aldrete scoreor = 9, pain immediately following emergence and PONV.Propofol-remifentanil combination effectively controlled responses to laryngoscopy and surgical stress. Drug related adverse events were transient bradycardia (50 bpm) and hypotension (SBPor = 80 mmHg) respectively: at prelaryngoscopy 11.60-1.48% and at pre-skin incision 10.61-0.98%. N. 365 patients were discharged from PACU and the median time to first Aldrete scoreor = 9 was 22.3 min. The most frequent postoperative event was shivering recorded in n. 46 patients (12%). Postoperative analgesic medication was requested by n. 16 patients (4.4%) and PONV was noted in n. 6 patients (1.6%).When combined with propofol, remifentanil effectively provided for profound analgesia during surgery, stable anesthetic conditions, simplicity of use and predictable recovery.
- Published
- 2000
9. [Foreign body inhalation in children. Report of a case]
- Author
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A, Pellegrino, C, Mendola, C, Ingolotti, and P, De Bellis
- Subjects
Male ,Radiography ,Child, Preschool ,Respiration ,Humans ,Foreign Bodies - Abstract
A case of inhalation of a foreign body in a child is reported. The case was recalcitant to treatment (antibiotics, bronchodilators, corticosteroids) and long-term follow-up is recommended.
- Published
- 1994
10. Characterization of 5'-flanking region of heart myosin light chain 2A gene. Structural and functional evidence for promoter activity
- Author
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K. Danishefsky, A Deshpande, M.A.Q. Siddiqui, D Nicholson, A.M. Zarraga, and C. Mendola
- Subjects
Preproinsulin ,Myosin light-chain kinase ,5' flanking region ,Nucleic acid sequence ,Promoter ,Cell Biology ,Biology ,Biochemistry ,Molecular biology ,Chloramphenicol acetyltransferase ,chemistry.chemical_compound ,chemistry ,Molecular Biology ,Gene ,DNA - Abstract
Two recombinant clones, lambda LC5 and lambda LC13, encompassing the entire regulatory myosin light chain 2 (MLC2A) gene of chicken heart muscle were isolated. Of these, lambda LC5 which contains a large 5'-flanking sequence of about 7.0 kb, was characterized by a partial nucleotide sequence analysis. A TATA-like sequence (TATTTTTA) and a CAAT-box (CAAAAGT) are located at positions -32 and -59, respectively, which most likely constitute the functional promoter region in the gene. Based on primer extension reaction with a synthetic 20-mer corresponding to the 5'-leader sequence and total poly(A+) RNA, the probable transcription initiation site in the gene was located. The gene promoter activity was demonstrated following transient expression of recombinant genomes containing the chicken upstream sequence fused to the bacterial chloramphenicol acetyltransferase (CAT) or to the rat preproinsulin II genes. The extracts from a Quail fibroblast cell line (QT35) transfected with the construct (pLCo5.2iCat) containing the putative chicken promoter, and the CAT gene promoted the formation of 3'-acetate chloramphenicol. Another construct (pBC12LC5.2f) contains the rat preproinsulin II gene placed under the control of chicken promoter and a simian virus 40 origin of replication. Transfection of COS cell line with pBC12LC5.2f DNA resulted in an efficient expression of rat preproinsulin mRNA initiating from the chicken promoter. The transfection assay also allowed detection of chicken MLC2A gene transcripts by S1-nuclease protection of end-labeled DNA probes. A comparison of the MLC2A upstream gene sequence with those available for skeletal myosin light chains revealed no common sequence elements, suggesting that cardiac MLC2A gene promoter region has diverged considerably from its counterparts in skeletal muscle.
- Published
- 1986
11. Expression of ventricular-type myosin light chain messenger RNA in spontaneously hypertensive rat atria
- Author
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Chandrika Saidapet, C. Mendola, Chandra Kumar, P. Delaney, and M.A.Q. Siddiqui
- Subjects
Gene isoform ,Male ,medicine.medical_specialty ,Myosin light-chain kinase ,Physiology ,Heart Ventricles ,Biology ,Myosins ,Rats, Inbred WKY ,Muscle hypertrophy ,Spontaneously hypertensive rat ,Internal medicine ,Rats, Inbred SHR ,Myosin ,medicine ,Animals ,cardiovascular diseases ,Heart Atria ,RNA, Messenger ,Pressure overload ,Messenger RNA ,Myocardium ,RNA ,Rats ,Endocrinology ,Hypertension ,cardiovascular system ,Cardiology and Cardiovascular Medicine - Abstract
Using cloned DNA probes specific for two isoforms of cardiac myosin light chains (MLCs), nonphosphorylatable MLC1 and phosphorylatable, regulatory MLC2, we have observed that the MLC1 messenger RNA of ventricular type does not appear in detectable amounts in atrial cells of either normotensive Wistar-Kyoto rat strain (WKY) or spontaneously hypertensive rat strain (SHR). The messenger RNA of regulatory isoform of ventricular MLC2, on the other hand, is found in threefold excess in atria of SHR relative to that of age-matched WKY. The increased level of MLC2 messenger RNA is present even in 6-week-old SHR atria where there is no established overloading of the heart. Thus, it appears that the increased expression of the regulatory MLC2 gene in SHR atrial cells is a predetermined event, which, most likely, participates in functional adaptation of the myocardium in response to pressure overload and subsequent hypertrophy.
- Published
- 1988
12. [Proposal for an anesthesiologic and integrated antalgic treatment for interventions of laparohysterectomy]
- Author
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A, Sicignano, G, Coven, D, Adduci, E, Domenegati, and C, Mendola
- Subjects
Adult ,Analgesia, Epidural ,Anesthesia, Epidural ,Random Allocation ,Anesthesia Recovery Period ,Humans ,Female ,Anesthesia, General ,Middle Aged ,Hysterectomy ,Aged - Published
- 1988
13. Co-ordinate control of gene expression. Muscle-specific 7 S RNA contains sequences homologous to 3'-untranslated regions of myosin genes and repetitive DNA
- Author
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P, Khandekar, C, Saidapet, M, Krauskopf, A M, Zarraga, W L, Lin, C, Mendola, and M A, Siddiqui
- Subjects
Electrophoresis, Agar Gel ,Base Sequence ,Transcription, Genetic ,Myocardium ,Nucleic Acid Hybridization ,Chick Embryo ,DNA ,Myosins ,Gene Expression Regulation ,RNA, Small Nuclear ,Animals ,RNA ,RNA, Messenger ,Plasmids ,Repetitive Sequences, Nucleic Acid - Abstract
We have cloned and sequenced a complementary DNA copy (pSS48) of a novel muscle-specific, low molecular weight RNA, 7 S RNA, isolated from embryonic chick cardiac muscle cells. The hybridization pattern of plasmid pSS48 DNA to chick genomic DNA suggests that 7 S RNA is derived from the repetitive chick DNA with a repetition frequency of about 300 copies per haploid genome. Under low stringency, pSS48 DNA also hybridizes with high specificity to the single copy gene for chick myosin light chain (MLC) and to myosin heavy chain (MHC), and possibly to other co-ordinately expressed genes for chick muscle proteins. The sequence analysis of recombinant plasmids pSS48, pML10 and pMHC8, for 7 S RNA, MLC mRNA and MHC RNA, respectively, indicated that short nucleotide stretches homologous to 7 S RNA reside in the 3' untranslated regions of the respective genes. The 7 S RNA sequence appears to be highly specific for the chick muscle tissue, since RNA and DNA from several sources did not hybridize to pSS48 DNA. Furthermore, the 7 S RNA-like sequence(s) appears in chick blastodermal cells preferentially earlier than the onset of transcription of genes for major muscle proteins. These results, taken together, suggest a possible function for 7 S RNA in expression of muscle-specific genes during chick development.
- Published
- 1984
14. [Intraoperative recovery of blood using a cell separator (Autotrans BT795/A) in vascular surgery and emergency traumatology]
- Author
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I, Preseglio, E, Domenegati, D, Adduci, R, Imberti, C, Mendola, and A, Mapelli
- Subjects
Adult ,Aged, 80 and over ,Male ,Intraoperative Care ,Multiple Trauma ,Aortic Rupture ,Centrifugation ,Cell Separation ,Middle Aged ,Aortic Aneurysm ,Blood Transfusion, Autologous ,Humans ,Female ,Aorta, Abdominal ,Aged - Published
- 1988
15. Characterization of 5'-flanking region of heart myosin light chain 2A gene. Structural and functional evidence for promoter activity
- Author
-
A M, Zarraga, K, Danishefsky, A, Deshpande, D, Nicholson, C, Mendola, and M A, Siddiqui
- Subjects
Base Sequence ,Transcription, Genetic ,Myocardium ,DNA, Recombinant ,Myosin Subfragments ,DNA Restriction Enzymes ,Myosins ,Peptide Fragments ,Genes ,Genes, Regulator ,Animals ,Amino Acid Sequence ,Cloning, Molecular ,Promoter Regions, Genetic ,Chickens - Abstract
Two recombinant clones, lambda LC5 and lambda LC13, encompassing the entire regulatory myosin light chain 2 (MLC2A) gene of chicken heart muscle were isolated. Of these, lambda LC5 which contains a large 5'-flanking sequence of about 7.0 kb, was characterized by a partial nucleotide sequence analysis. A TATA-like sequence (TATTTTTA) and a CAAT-box (CAAAAGT) are located at positions -32 and -59, respectively, which most likely constitute the functional promoter region in the gene. Based on primer extension reaction with a synthetic 20-mer corresponding to the 5'-leader sequence and total poly(A+) RNA, the probable transcription initiation site in the gene was located. The gene promoter activity was demonstrated following transient expression of recombinant genomes containing the chicken upstream sequence fused to the bacterial chloramphenicol acetyltransferase (CAT) or to the rat preproinsulin II genes. The extracts from a Quail fibroblast cell line (QT35) transfected with the construct (pLCo5.2iCat) containing the putative chicken promoter, and the CAT gene promoted the formation of 3'-acetate chloramphenicol. Another construct (pBC12LC5.2f) contains the rat preproinsulin II gene placed under the control of chicken promoter and a simian virus 40 origin of replication. Transfection of COS cell line with pBC12LC5.2f DNA resulted in an efficient expression of rat preproinsulin mRNA initiating from the chicken promoter. The transfection assay also allowed detection of chicken MLC2A gene transcripts by S1-nuclease protection of end-labeled DNA probes. A comparison of the MLC2A upstream gene sequence with those available for skeletal myosin light chains revealed no common sequence elements, suggesting that cardiac MLC2A gene promoter region has diverged considerably from its counterparts in skeletal muscle.
- Published
- 1986
16. Function analysis of heart myosin light chain-2 gene promoter by site specific mutagenesis
- Author
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K. Datta, M.A.Q. Siddiqui, C. Mendola, A.M. Zarraga, K. Danishefsky, and M. Gadot
- Subjects
Function analysis ,Myosin light-chain kinase ,Chemistry ,Promoter ,Cardiology and Cardiovascular Medicine ,Site-directed mutagenesis ,Molecular Biology ,Cell biology - Published
- 1987
17. Effects of the benzodiazepine inverse agonist FG7142 on the structure of anxiety-related behavior of male Wistar rats tested in hole board.
- Author
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Casarrubea M, Faulisi F, Pensabene M, Mendola C, Dell'Utri R, Cardaci M, Santangelo A, and Crescimanno G
- Subjects
- Animals, Carrier Proteins agonists, Drug Inverse Agonism, Environment, Male, Motor Activity drug effects, Rats, Rats, Wistar, Receptors, GABA-A, Anxiety, Behavior, Animal drug effects, Carbolines pharmacology, Exploratory Behavior drug effects, GABA Antagonists pharmacology
- Abstract
Rationale: Little is known about the structural characteristics of the behavior of rats with enhanced anxiety level. To fill this gap, a study was undertaken where effects of an anxiogenic drug were examined on behavioral structure of rats tested in hole board., Objectives: This study investigates effects of increased anxiety level on the structure of the behavior of rats tested in hole board METHODS: Different doses of FG7142 (1, 4, 8 mg/kg IP), a potent anxiety-inducing drug, were administered to three groups of male Wistar rats. A further group was administered saline. Experiments were recorded through a digital camera. Quantitative and multivariate approaches were applied., Results: Percent distributions and durations showed increases of immobile sniffing, rearing, head dip, and edge sniff and a significant reduction of grooming activities and of walking. In addition, a decrease of head dip/edge sniff ratio was detected. Transition matrices evidenced that FG7142 provoked evident modifications of behavioral structure mainly of general exploration of environment and focused exploration of the hole. Finally, adjusted residuals showed a reduced effectiveness of FG7142 on transitions from head dip to edge sniff; on the contrary, transitions from edge sniff to head dip underwent evident dose-dependent changes., Conclusions: Present study provides a useful tool to analyze behavioral responses to different anxiety conditions. Accordingly, it is demonstrated that a condition of increased anxiety deeply modifies the structure of male Wistar rat's behavior in hole board. In addition, our results suggest that evaluation of head dip/edge sniff ratio can be considered a reliable index to appraise effects of pharmacological manipulation of anxiety and related behavioral elements.
- Published
- 2017
- Full Text
- View/download PDF
18. High-dose rocuronium for rapid-sequence induction and reversal with sugammadex in two myasthenic patients.
- Author
-
Casarotti P, Mendola C, Cammarota G, and Della Corte F
- Subjects
- Aged, Female, Humans, Male, Middle Aged, Neuromuscular Blockade methods, Neuromuscular Monitoring methods, Rocuronium, Sugammadex, Androstanols antagonists & inhibitors, Androstanols therapeutic use, Myasthenia Gravis surgery, Neuromuscular Nondepolarizing Agents antagonists & inhibitors, Neuromuscular Nondepolarizing Agents therapeutic use, gamma-Cyclodextrins therapeutic use
- Abstract
The anesthetic management of patients affected by myasthenia gravis is usually challenging in elective surgery and even more so in emergency procedures. The difficulties involved are several-fold, ranging from the choice of an appropriate muscle relaxant (i.e. one that enables safe and rapid airway management) to neuromuscular monitoring and normal muscular recovery. Additionally, optimizing patient conditions - either pharmacologically or with plasmapheresis - before intervention is well beyond the realm of possibility. We discuss the anesthetic management of two myasthenic patients undergoing emergency surgery (for sigmoid perforation and upper gastrointestinal bleeding respectively). In both cases, we opted for rapid-sequence induction with high-dose rocuronium to prevent inhalation of gastric contents. We also report on the implication of neuromuscular monitoring. We found that the rocuronium-sugammadex combination was a useful and effective option in the emergency setting., (© 2014 The Acta Anaesthesiologica Scandinavica Foundation. Published by John Wiley & Sons Ltd.)
- Published
- 2014
- Full Text
- View/download PDF
19. Reversal of rocuronium induced neuromuscular block with sugammadex or neostigmine: a large observational study.
- Author
-
Della Rocca G, Pompei L, Pagan DE Paganis C, Tesoro S, Mendola C, Boninsegni P, Tempia A, Manstretta S, Zamidei L, Gratarola A, Murabito P, Fuggiano L, and DI Marco P
- Subjects
- Abdomen surgery, Adult, Aged, Airway Extubation, Analgesics, Opioid therapeutic use, Anesthesia Recovery Period, Anesthesia, General, Endpoint Determination, Female, Humans, Male, Middle Aged, Movement drug effects, Neostigmine administration & dosage, Neostigmine adverse effects, Neuromuscular Blockade, Parasympathomimetics administration & dosage, Parasympathomimetics adverse effects, Postoperative Complications prevention & control, Prospective Studies, Recovery Room, Rocuronium, Sample Size, Sugammadex, gamma-Cyclodextrins administration & dosage, gamma-Cyclodextrins adverse effects, Androstanols antagonists & inhibitors, Neostigmine therapeutic use, Neuromuscular Nondepolarizing Agents antagonists & inhibitors, Parasympathomimetics therapeutic use, gamma-Cyclodextrins therapeutic use
- Abstract
Background: This 'real-life' study aimed to analyze the time from the start of neostigmine or sugammadex administration to recovery to a train of four ratio (TOFr) of 0.9 in a real-life in patients receiving rocuronium. The secondary aims were to assess the proportion of patients: presenting TOFr < 0.9 after 5, 10, and 20 min from reversal agent administration, receiving opioids for intraoperative analgesia and extubated in the post-anesthesia care unit (PACU)., Methods: This was a multisite, prospective, nonrandomized, observational real-life study. Reversal agent was administered at either T2 reappearance or at a post-tetanic count of 1 or 2. Drugs dosages were free according to each investigator's usual practice., Results: Three hundred fifty-nine patients were enrolled onto the study. Time from reversal administration to TOFr to 0.9 is significantly faster in the sugammadex group than in the neostigmine group (shallow block: 2.2 vs. 6.9 min, respectively; P < 0.0001; deep block: 2.7 vs. 16.2 min, respectively; P < 0.0001). The number of patients with TOFr < 0.9 at 5, 10, and 20 min post-reversal agent administration was higher in the neostigmine than in the sugammadex group. Just five patients did not receive opioids. All patients were extubated in the operative room except for a single patient in the sugammadex group who was extubated following PACU admission., Conclusions: This real-life study confirms that reversal time is faster in patients receiving sugammadex than in those receiving neostigmine. TOFr < 0.9 20 min after reversal was only present in patients treated with neostigmine., (© 2013 The Acta Anaesthesiologica Scandinavica Foundation. Published by John Wiley & Sons Ltd.)
- Published
- 2013
- Full Text
- View/download PDF
20. S(+)-ketamine for control of perioperative pain and prevention of post thoracotomy pain syndrome: a randomized, double-blind study.
- Author
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Mendola C, Cammarota G, Netto R, Cecci G, Pisterna A, Ferrante D, Casadio C, and Della Corte F
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Analgesia, Epidural, Double-Blind Method, Female, Follow-Up Studies, Humans, Male, Middle Aged, Pain Measurement, Pain, Postoperative epidemiology, Perioperative Care, Prospective Studies, Young Adult, Anesthetics, Dissociative therapeutic use, Intraoperative Complications prevention & control, Ketamine therapeutic use, Pain, Postoperative prevention & control, Thoracotomy adverse effects
- Abstract
Background: Post-thoracotomy pain syndrome (PTPS) often complicates the long term outcome of patients; its appearance has been related to perioperative acute pain. The main goal of this study was to evaluate a possible role of S(+)-ketamine in the prevention of PTPS up to 6 months and secondarily its efficacy in the control of perioperative pain when added to thoracic epidural analgesia (TEA) and adjuvants., Methods: Sixty-six patients underwent thoracotomy under general anesthesia. A thoracic epidural catheter was placed for levobupivacaine and sufentanil administration. Thirty-three patients received an i.v. infusion of S(+)-ketamine (Group S(+)K) for 60 hours and 33 patients received i.v. placebo (Group PLAC). Pain was evaluated by Numeric Rating Scale (NRS) during the whole study. All patients had supplementary doses of analgesics, as needed, to have NRS targeted to a value of ≤3 in the 1st and <3 in the following days. Neuropathic Pain Symptom Inventory (NPSI) was evaluated at 1, 3 and 6 months., Results: All patients had NRS ≤3 in the early postoperative period and NPSI was less or equal to 1 in the follow-up control for each group with no significant difference at three (P=0.67, OR 0.8 [IC95% 0.3-2.2]) and at six months (P=0.23, OR 1.9 [0.7-5.4]). Incidence of moderate PTPS was 24.6% at 3 and 21.1% at six months while severe PTPS was 6.6% at 3 and 1.8% at six months. No difference was detected in NRS and NPSI at 3 and 6 months between groups., Conclusion: S(+)-ketamine had no effects in respect to placebo in the prevention of PTPS at 3 and 6 months but had a significant role in maintaining a NRS≤3 in the early postoperative period. A tight control of perioperative pain seems to be associated with a low incidence of moderate and severe PTPS.
- Published
- 2012
21. Thoracic epidural analgesia in post-thoracotomy patients: comparison of three different concentrations of levobupivacaine and sufentanil.
- Author
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Mendola C, Ferrante D, Oldani E, Cammarota G, Cecci G, Vaschetto R, and Della Corte F
- Subjects
- Adult, Aged, Aged, 80 and over, Analgesia, Epidural adverse effects, Analgesia, Epidural methods, Analgesics, Opioid adverse effects, Anesthetics, Local adverse effects, Bupivacaine administration & dosage, Bupivacaine adverse effects, Bupivacaine analogs & derivatives, Double-Blind Method, Drug Administration Schedule, Female, Humans, Hypotension chemically induced, Levobupivacaine, Male, Middle Aged, Pain Measurement methods, Pain, Postoperative prevention & control, Postoperative Nausea and Vomiting chemically induced, Prospective Studies, Pruritus chemically induced, Sufentanil adverse effects, Analgesics, Opioid administration & dosage, Anesthetics, Local administration & dosage, Sufentanil administration & dosage, Thoracotomy
- Abstract
Background: Relative effects of dosage, volume and concentration of local anaesthetics used for postoperative thoracic epidural analgesia are still under debate. In this randomized, prospective, double-blinded study, we evaluated the incidence of side-effects such as changes in arterial pressure, postoperative nausea, vomiting, and pruritus in patients admitted for thoracic surgery during continuous thoracic epidural infusion using levobupivacaine and sufentanil mixture in three different volumes., Methods: We studied 150 patients who underwent thoracotomy with a thoracic epidural catheter placed between T4 and T7. The patients were randomized into three groups which received 10 mg h(-1) of levobupivacaine at three different concentrations (0.5%, 0.25%, and 0.15%), in combination with sufentanil at 2.6 microg h(-1). Haemodynamic effects, pruritus, nausea, vomiting, sensory and motor block, pain score, additional analgesic requirement, sedation, and patient satisfaction were registered immediately after the surgical operation and on the first, second, and third postoperative days., Results: We did not detect any differences in the incidence of side-effects such as changes in arterial pressure, and also postoperative nausea, vomiting, and pruritus. The three groups were also similar with regard to patient characteristics, sensory and motor block, pain score, analgesic rescue dose, sedation, and patient satisfaction., Conclusions: The same dose of a mixture of levobupivacaine and sufentanil administered in three different volumes and concentrations during continuous thoracic epidural infusion for thoracotomy provided an equal incidence of adverse haemodynamic effects, nausea, vomiting, or pruritus.
- Published
- 2009
- Full Text
- View/download PDF
22. Cerebral cavernomas and seizures: a retrospective study on 163 patients who underwent pure lesionectomy.
- Author
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Ferroli P, Casazza M, Marras C, Mendola C, Franzini A, and Broggi G
- Subjects
- Adolescent, Adult, Brain Neoplasms complications, Female, Hemangioma, Cavernous complications, Humans, Magnetic Resonance Imaging methods, Male, Middle Aged, Retrospective Studies, Seizures etiology, Brain Neoplasms surgery, Hemangioma, Cavernous surgery, Microsurgery methods, Neurosurgical Procedures methods, Seizures surgery, Treatment Outcome
- Abstract
The objective was to evaluate the outcome of microsurgical "pure" lesionectomy in patients with supratentorial cavernous angiomas presenting with seizures. For this retrospective study 163 patients with cavernoma-related epileptic seizures were selected. They all underwent surgery in a single institution between 1988 and 2003. A microsurgical frame/frameless guided minimally invasive transulcal "pure" lesionectomy was performed. The haemosiderin stained gliotic brain parenchyma that was usually found surrounding the lesion was not removed. Among the 99 patients with epilepsy and longer clinical history, 68 (68.7%) were found completely to be seizure-free, 10 (10.1%) presented sporadic and less frequent seizures and 17 (17.1%) remained unchanged. Sixty-three out of 64 (98.4%) patients who experienced only single or sporadic seizures were found to be completely seizure-free after surgery. Five patients were lost at follow-up (mean 48 months, range 0.5-14 years). Long-term morbidity was 1.8%. Mortality was null. No haemorrhagic episodes were observed during follow-up. Pure lesionectomy prevents bleeding and development of epilepsy in patients that receive early surgery after the epileptic onset. In most of the epileptic patients with a good concordance between the electroclinical data and the location of the angioma, good results can be achieved by this kind of surgery so that more invasive and costly studies to find and remove the epileptogenic cerebral parenchyma seem justified only after lesionectomy fails.
- Published
- 2006
- Full Text
- View/download PDF
23. [The clinical course and surgical indications (the RAND Corporation method].
- Author
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Corona C, Irace C, Giannachi L, Mendola C, and Usai S
- Subjects
- Diagnostic Imaging, Disease Progression, Evidence-Based Medicine, Humans, Intervertebral Disc Displacement complications, Intervertebral Disc Displacement diagnosis, Intervertebral Disc Displacement physiopathology, Intervertebral Disc Displacement surgery, Intraoperative Complications epidemiology, Low Back Pain diagnosis, Low Back Pain etiology, Low Back Pain surgery, Lumbar Vertebrae surgery, Physical Examination methods, Postoperative Complications epidemiology, Practice Guidelines as Topic, Randomized Controlled Trials as Topic, Retrospective Studies, Sciatic Neuropathy diagnosis, Sciatic Neuropathy etiology, Sciatic Neuropathy surgery, Spinal Stenosis complications, Spinal Stenosis diagnosis, Spinal Stenosis physiopathology, Spinal Stenosis surgery, Unnecessary Procedures, Algorithms, Diskectomy adverse effects, Diskectomy statistics & numerical data, Low Back Pain physiopathology, Sciatic Neuropathy physiopathology, Severity of Illness Index
- Published
- 2006
24. The role of intraoperative electromyographic monitoring in lumbosacral lipomas.
- Author
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Valentini LG, Visintini S, Mendola C, Casali C, Bono R, Scaioli W, and Solero CL
- Subjects
- Adolescent, Adult, Child, Child, Preschool, Female, Follow-Up Studies, Humans, Infant, Lipoma complications, Lipoma diagnosis, Lipoma physiopathology, Male, Middle Aged, Neural Tube Defects etiology, Retrospective Studies, Spinal Cord Neoplasms diagnosis, Spinal Cord Neoplasms physiopathology, Treatment Outcome, Electromyography, Lipoma surgery, Lumbar Vertebrae, Monitoring, Intraoperative methods, Neurosurgical Procedures adverse effects, Sacrum, Spinal Cord Neoplasms surgery
- Abstract
Objective: To demonstrate the role of intraoperative multichannel electromyographic (EMG) monitoring to reduce postoperative deterioration and achieve full untethering of complex occult dysraphisms., Methods: A retrospective analysis was performed on 66 patients who underwent operation for lumbosacral lipomas. Twenty recent cases were submitted to EMG monitoring and stimulation., Results: All patients presented symptoms at the time of surgery, and 74% exhibited progressive deterioration during the lengthy preoperative period. Postoperative surgery-related deterioration was observed in 6% of patients. This number was reduced to zero with the introduction of intraoperative EMG monitoring., Conclusion: Intraoperative multichannel EMG monitoring can be carried out and requires only minimal changes to anesthetic procedures. With this method, it is possible to better identify the neural structures of complex malformations, reducing the risks of surgical damage and incomplete detethering.
- Published
- 2005
- Full Text
- View/download PDF
25. Craniotomies without burr holes using an oscillating saw.
- Author
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DiMeco F, Li KW, Mendola C, Cantú G, and Solero CL
- Subjects
- Brain Neoplasms surgery, Humans, Skull Base surgery, Skull Base Neoplasms surgery, Craniotomy instrumentation, Surgical Instruments
- Abstract
Objective: The object of this study is to describe a new method for performing craniotomies which obviates the need for burr holes, improves bony reconstruction, and reduces post-operative cosmetic deformities. Moreover, this technique provides excellent exposure of skull base structures and dural venous sinuses., Methods: Craniotomies in varied locations are created with the use of a micro-oscillating saw and chisel. No burr holes are used and reconstruction with plates and screws is unnecessary., Results: We initially applied our technique to approaches to the anterior skull base with combined craniofacial tumour resections. We have since performed over 2000 craniotomies of any size and shape in all supratentorial locations using the oscillating saw., Conclusions: We have found that our method creates better cosmetic results than standard techniques and is safer for craniotomies spanning dural venous sinuses. With experience, operating time was significantly reduced and costs were lowered because reconstruction with fixation devices was not needed.
- Published
- 2004
- Full Text
- View/download PDF
26. Immunotherapy and biological modifiers for the treatment of malignant brain tumors.
- Author
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Marras C, Mendola C, Legnani FG, and DiMeco F
- Subjects
- Animals, Biopsy, Needle, Brain Neoplasms mortality, Clinical Trials as Topic, Cytokines analysis, Cytokines metabolism, Disease Models, Animal, Female, Glioma mortality, Histocompatibility, Humans, Interferons analysis, Interferons metabolism, Male, Neoplasm Staging, Prognosis, Sensitivity and Specificity, Survival Analysis, Telomerase analysis, Telomerase metabolism, Treatment Outcome, Biomarkers analysis, Brain Neoplasms pathology, Brain Neoplasms therapy, Glioma pathology, Glioma therapy, Immunotherapy methods
- Abstract
The relative ineffectiveness of current therapies for malignant gliomas has led to the need for novel therapeutics. Therapies based on biologic modifiers are among a variety of cancer treatments currently in use or under experimental evaluation and have shown great promise, especially since several potent stimulators of the immune system have been cloned and are now available for clinical use. Early attempts at glioma therapy based on biologic modifiers, however, have failed to demonstrate significant effectiveness. In this review, we select and summarize the results of preclinical and clinical studies published during the past two years that focus on immunotherapy and biologic modifiers for treating gliomas. Despite limited clinical success, we conclude that an increased understanding of molecular biology and immunology from recent studies may pave the way for more effective approaches.
- Published
- 2003
- Full Text
- View/download PDF
27. Total intravenous anesthesia with propofol and remifentanil for elective non-cardiac surgery.
- Author
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Pelosi G, Gratarola A, Pissaia C, Mendola C, and Bellomo G
- Subjects
- Female, Humans, Male, Middle Aged, Remifentanil, Anesthesia, Intravenous adverse effects, Anesthetics, Combined, Anesthetics, Intravenous, Elective Surgical Procedures, Piperidines, Propofol
- Abstract
Background: Remifentanil is a highly effective mu opioid agonist with predictable pharmacokinetics and a close concentration-effect relationship. Moreover, studies on anesthetic drugs interactions show that optimal propofol concentrations decrease more significantly with remifentanil as compared with other opioids and recovery appears to be much faster than when propofol is combined with other opioids combinations. This intervention study was designed to evaluate the efficacy of propofol combined with remifentanil in elective non cardiac inpatient surgery., Methods: N. 405 patients undergoing intraperitoneal, head-neck, intrathoracic, major orthopaedics, breast and major vascular surgery received: remifentanil (1 microgram.kg-1 at induction; 0.50 microgram.kg-1.min-1 at laryngoscopy; 0.25 microgram.kg-1.min-1 at skin incision; 0.25-0.30 microgram.kg-1.min1 from skin incision to end of skin suture) and propofol (0.5-1 mg.kg-1 at induction; 5 mg.kg-1.h-1 at laryngoscopy; 5 mg.kg-1.h-1 at skin incision and 5 mg.kg1.h1 thereafter). Intraoperative end-points included somatic responses, tachycardia and hypertension to laryngoscopy and surgery. Incidence of intraoperative bradycardia, hypotension and muscle rigidity were also recorded. Postoperative end-points included Aldrete score > or = 9, pain immediately following emergence and PONV., Results: Propofol-remifentanil combination effectively controlled responses to laryngoscopy and surgical stress. Drug related adverse events were transient bradycardia (< 50 bpm) and hypotension (SBP < or = 80 mmHg) respectively: at prelaryngoscopy 11.60-1.48% and at pre-skin incision 10.61-0.98%. N. 365 patients were discharged from PACU and the median time to first Aldrete score > or = 9 was 22.3 min. The most frequent postoperative event was shivering recorded in n. 46 patients (12%). Postoperative analgesic medication was requested by n. 16 patients (4.4%) and PONV was noted in n. 6 patients (1.6%)., Conclusions: When combined with propofol, remifentanil effectively provided for profound analgesia during surgery, stable anesthetic conditions, simplicity of use and predictable recovery.
- Published
- 1999
28. [Foreign body inhalation in children. Report of a case].
- Author
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Pellegrino A, Mendola C, Ingolotti C, and De Bellis P
- Subjects
- Child, Preschool, Humans, Male, Radiography, Respiration, Foreign Bodies diagnostic imaging
- Abstract
A case of inhalation of a foreign body in a child is reported. The case was recalcitant to treatment (antibiotics, bronchodilators, corticosteroids) and long-term follow-up is recommended.
- Published
- 1994
29. Tissue specificity of 3'-untranslated sequence of myosin light chain gene: unexpected interspecies homology with repetitive DNA.
- Author
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Saidapet C, Khandekar P, Mendola C, and Siddiqui MA
- Subjects
- Animals, Cattle, Chick Embryo, Cloning, Molecular, Dictyostelium genetics, Escherichia coli genetics, Euglena genetics, Genes, HeLa Cells, Humans, Muscles metabolism, Myocardium metabolism, Nucleic Acid Hybridization, Organ Specificity, Plasmids, Protein Biosynthesis, Rats, Repetitive Sequences, Nucleic Acid, Species Specificity, Xenopus genetics, Zea mays genetics, DNA genetics, Myosins genetics, RNA, Messenger genetics
- Abstract
Using the 3' noncoding and coding sequences of chick heart myosin light chain mRNA cloned into Escherichia coli as probes, it was observed that, while the coding sequence shared homology with myosin light-chain mRNAs from other sources, the 3' noncoding sequence was specific for chick heart muscle. This property was used to detect chick heart-specific myosin light-chain gene activity in chick blastoderms of very early developmental stages where cells of different muscle origins cannot be distinguished morphologically. However, in spite of the tissue-specific divergence of the 3' noncoding sequence of myosin light-chain gene, which is present in a single copy in the chick genome, a surprising homology with DNA from such a diverse source like Dictyostelium discoideum was noted. The sequence homologous to chick myosin light-chain DNA was apparently present in a high repetition frequency in the Dictyostelium genome.
- Published
- 1984
- Full Text
- View/download PDF
30. [Intraoperative recovery of blood using a cell separator (Autotrans BT795/A) in vascular surgery and emergency traumatology].
- Author
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Preseglio I, Domenegati E, Adduci D, Imberti R, Mendola C, and Mapelli A
- Subjects
- Adult, Aged, Aged, 80 and over, Aorta, Abdominal surgery, Aortic Rupture surgery, Blood Transfusion, Autologous methods, Centrifugation, Female, Humans, Male, Middle Aged, Aortic Aneurysm surgery, Blood Transfusion, Autologous instrumentation, Cell Separation instrumentation, Intraoperative Care, Multiple Trauma surgery
- Published
- 1988
31. Co-ordinate control of gene expression. Muscle-specific 7 S RNA contains sequences homologous to 3'-untranslated regions of myosin genes and repetitive DNA.
- Author
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Khandekar P, Saidapet C, Krauskopf M, Zarraga AM, Lin WL, Mendola C, and Siddiqui MA
- Subjects
- Animals, Base Sequence, Chick Embryo, Electrophoresis, Agar Gel, Myocardium analysis, Nucleic Acid Hybridization, Plasmids, RNA, Messenger, RNA, Small Nuclear, Transcription, Genetic, DNA, Gene Expression Regulation, Myosins genetics, RNA genetics, Repetitive Sequences, Nucleic Acid
- Abstract
We have cloned and sequenced a complementary DNA copy (pSS48) of a novel muscle-specific, low molecular weight RNA, 7 S RNA, isolated from embryonic chick cardiac muscle cells. The hybridization pattern of plasmid pSS48 DNA to chick genomic DNA suggests that 7 S RNA is derived from the repetitive chick DNA with a repetition frequency of about 300 copies per haploid genome. Under low stringency, pSS48 DNA also hybridizes with high specificity to the single copy gene for chick myosin light chain (MLC) and to myosin heavy chain (MHC), and possibly to other co-ordinately expressed genes for chick muscle proteins. The sequence analysis of recombinant plasmids pSS48, pML10 and pMHC8, for 7 S RNA, MLC mRNA and MHC RNA, respectively, indicated that short nucleotide stretches homologous to 7 S RNA reside in the 3' untranslated regions of the respective genes. The 7 S RNA sequence appears to be highly specific for the chick muscle tissue, since RNA and DNA from several sources did not hybridize to pSS48 DNA. Furthermore, the 7 S RNA-like sequence(s) appears in chick blastodermal cells preferentially earlier than the onset of transcription of genes for major muscle proteins. These results, taken together, suggest a possible function for 7 S RNA in expression of muscle-specific genes during chick development.
- Published
- 1984
- Full Text
- View/download PDF
32. Characterization of 5'-flanking region of heart myosin light chain 2A gene. Structural and functional evidence for promoter activity.
- Author
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Zarraga AM, Danishefsky K, Deshpande A, Nicholson D, Mendola C, and Siddiqui MA
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, Chickens, Cloning, Molecular, DNA Restriction Enzymes, DNA, Recombinant metabolism, Genes, Regulator, Myocardium metabolism, Myosin Subfragments, Transcription, Genetic, Genes, Myosins genetics, Peptide Fragments genetics, Promoter Regions, Genetic
- Abstract
Two recombinant clones, lambda LC5 and lambda LC13, encompassing the entire regulatory myosin light chain 2 (MLC2A) gene of chicken heart muscle were isolated. Of these, lambda LC5 which contains a large 5'-flanking sequence of about 7.0 kb, was characterized by a partial nucleotide sequence analysis. A TATA-like sequence (TATTTTTA) and a CAAT-box (CAAAAGT) are located at positions -32 and -59, respectively, which most likely constitute the functional promoter region in the gene. Based on primer extension reaction with a synthetic 20-mer corresponding to the 5'-leader sequence and total poly(A+) RNA, the probable transcription initiation site in the gene was located. The gene promoter activity was demonstrated following transient expression of recombinant genomes containing the chicken upstream sequence fused to the bacterial chloramphenicol acetyltransferase (CAT) or to the rat preproinsulin II genes. The extracts from a Quail fibroblast cell line (QT35) transfected with the construct (pLCo5.2iCat) containing the putative chicken promoter, and the CAT gene promoted the formation of 3'-acetate chloramphenicol. Another construct (pBC12LC5.2f) contains the rat preproinsulin II gene placed under the control of chicken promoter and a simian virus 40 origin of replication. Transfection of COS cell line with pBC12LC5.2f DNA resulted in an efficient expression of rat preproinsulin mRNA initiating from the chicken promoter. The transfection assay also allowed detection of chicken MLC2A gene transcripts by S1-nuclease protection of end-labeled DNA probes. A comparison of the MLC2A upstream gene sequence with those available for skeletal myosin light chains revealed no common sequence elements, suggesting that cardiac MLC2A gene promoter region has diverged considerably from its counterparts in skeletal muscle.
- Published
- 1986
33. Expression of ventricular-type myosin light chain messenger RNA in spontaneously hypertensive rat atria.
- Author
-
Kumar C, Saidapet C, Delaney P, Mendola C, and Siddiqui MA
- Subjects
- Animals, Heart Atria, Heart Ventricles, Male, Myosins classification, Rats, Rats, Inbred SHR, Rats, Inbred WKY, Hypertension metabolism, Myocardium enzymology, Myosins genetics, RNA, Messenger metabolism
- Abstract
Using cloned DNA probes specific for two isoforms of cardiac myosin light chains (MLCs), nonphosphorylatable MLC1 and phosphorylatable, regulatory MLC2, we have observed that the MLC1 messenger RNA of ventricular type does not appear in detectable amounts in atrial cells of either normotensive Wistar-Kyoto rat strain (WKY) or spontaneously hypertensive rat strain (SHR). The messenger RNA of regulatory isoform of ventricular MLC2, on the other hand, is found in threefold excess in atria of SHR relative to that of age-matched WKY. The increased level of MLC2 messenger RNA is present even in 6-week-old SHR atria where there is no established overloading of the heart. Thus, it appears that the increased expression of the regulatory MLC2 gene in SHR atrial cells is a predetermined event, which, most likely, participates in functional adaptation of the myocardium in response to pressure overload and subsequent hypertrophy.
- Published
- 1988
- Full Text
- View/download PDF
34. [Proposal for an anesthesiologic and integrated antalgic treatment for interventions of laparohysterectomy].
- Author
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Sicignano A, Coven G, Adduci D, Domenegati E, and Mendola C
- Subjects
- Adult, Aged, Anesthesia Recovery Period, Female, Humans, Middle Aged, Random Allocation, Analgesia, Epidural methods, Anesthesia, Epidural methods, Anesthesia, General methods, Hysterectomy methods
- Published
- 1988
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