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Introduction: The quality of life of children with sickle cell disease (SCD) depends on the severity, timing and number of painful episodes (vaso-occlusive crises, VOC) and their need for medical treatment and hospitalizations. The objective of this study was to explore the experiences of pediatric patients and their families during VOC. Methods: This qualitative study used semi-structured one-on-one and focus group interviews, designed in partnership with two patients and one parent, in a single center, tertiary care pediatric university-affiliated hospital. Two groups of participants were interviewed independently: (1) adolescent patients aged 12 to 18 years old hospitalised within the last 2 years for VOC, (2) parents of pediatric patients with SCD hospitalised within the last 2 years for VOC. Data was transcribed in full and analysed using NVivo12. Descriptive thematic content analysis was performed by coding themes emerging from data. After validating codes through interjudge assessment by consensus, themes from teenagers’ and parent's discourses were systematically compared for the final analysis. Results: Between June and August 2018, 8 interviews were conducted. 10 parents and 5 adolescents participated. Teenagers’ and parents’ answers mirrored each other's. Prompt pain relief was crucial, although the side effects of pain relief medications used were an added source of suffering. Recent quality improvement initiatives such as standardised order sheets were noteworthy improvements, though personalizing care to each patient's with pharmacological and non-pharmacological methods was also important to participants. Given the unpredictability and severity of VOC, their impact on both patients’ and families’ lives were substantial, as was the long term emotional burden. Parents felt guilty given the hereditary nature of the disease, they encouraged neonatal and prenatal testing, and they sought definitive treatments for both VOC and SCD. Tensions within parent-teenager relationships were described centered on developing autonomy and protecting the child to improve adherence to treatments. Conclusion: Participants emphasized the need to provide timely adequate analgesia, through both standardised quality improvement initiatives and a personalised approach to analgesia. Understanding the impact of VOC on patients’ lives and their socio-familial context is important to tailor clinical interventions.

Crenosoma vulpis, the fox lungworm, infects wild and domestic canids and is a cause of chronic respiratory disease in dogs in North America and Europe. The objective of this study was to determine the efficacy of milbemycin oxime (0.5mg/kg)/praziquantel (5mg/kg) (Milbemax; Novartis Animal Health, Inc.) against C. vulpis infection in a randomized, blinded, placebo-controlled study using experimentally infected dogs. Sixteen beagles (8 males, 8 females) were each given 100 infective third-stage larvae of C. vulpis. Fecal samples were examined for first-stage larvae by quantitative Baermann examination pre-exposure and at days 21, 28, 35, 42 and 49 post-infection (PI). All of the dogs were shedding larvae in the feces at 21 days PI. The dogs were randomly assigned to one of two groups. At 28 days PI, Group 1 (4 males, 4 females) received placebo only while Group 2 (4 males, 4 females) received a single treatment of milbemycin oxime (0.5mg/kg) and praziquantel (5mg/kg). The 16 dogs were euthanized and necropsied at 49 days PI. Lungs were removed, assessed for gross lesions (graded on a subjective scale 0-3 with 0 being normal) and C. vulpis were collected by lung-flush and counted. Samples of lung tissue were preserved for evaluation of histopathology and the lesions graded on a subjective scale (0-3 with 0 being normal). Gross and histopathology lesions were detected in all 8 untreated Group 1 dogs with mean subjective lesion scores of 1.8 ± 0.7 (range 1-3) and 3.0 ± 0.0 (range 3), respectively. Gross lesions were observed in 3/8 and histopathology lesions in all 8 of the treated Group 2 dogs with mean subjective lesion scores of 0.4 ± 0.5 (range 0-1) and 1.3 ± 0.4 (range 1-2), respectively. The mean (geometric) number for adult C. vulpis recovered in untreated dogs was 48.3 (range 25-70) compared with 0.65 (range 0-2) in animals treated with Milbemax. The resulting efficacy against C. vulpis was 98.7%. The number of C. vulpis was significantly lower for treated dogs than the burden in the untreated group (p=0.0002). A single dose of Milbemax (milbemycin oxime 0.5mg/kg+praziquantel 5mg/kg) was highly effective for the treatment of patent C. vulpis infection in dogs. A dosing interval for the prevention of clinical disease in dogs exposed to natural infections has not been established.

Fifty-six dogs from St. John's, Newfoundland, Canada, were evaluated for Angiostrongylus vasorum infection. Small numbers of nematodes were found within pulmonary arteries of 6 dogs. Larvae were identified in fecal samples in 2 of 6 dogs. All 6 dogs had multifocal granulomatous pneumonia and sometimes foci of chronic thrombosis, which varied from very mild to severe. One dog had extensive pulmonary lesions resulting in cor pulmonale. Right heart failure was characterized by right ventricular hypertrophy, hepatic congestion, ascites, and hydrothorax. Microscopically, in most cases, eggs, larvae, and sometimes intravascular adults, were present within lung tissue sections. Small foci of granulomatous inflammation with and without larvae were present in kidney and brain in 4 dogs. An additional dog, diagnosed antemortem with angiostrongylosis via fecal examination, was also examined. Pathological findings consisted of severe pyogranulomatous interstitial pneumonia with myriad eggs, larvae, and numerous intravascular pulmonary adult nematodes with extensive arterial thrombosis. Five hundred and seventy-two adult worms were removed from pulmonary arteries. Foci of granulomatous inflammation, often associated with larvae and/or eggs, were present in tracheobronchial lymph nodes, adrenal gland, brain, and kidneys. Severe seizuring noted antemortem was attributed to several large, discrete areas of acute hemorrhagic infarction within the cerebrum and cerebellum. Natural A. vasorum infection in domestic dogs in eastern Newfoundland causes lung pathology of variable severity, which in some cases, may progress to cor pulmonale and which may be associated with extrapulmonary lesions and clinical signs.

This report details 2 outbreaks of dermatophytosis in 2 different mink ranches. On the first farm, only kits were affected, while on the second farm, small numbers of adults were infected. Affected mink were otherwise clinically healthy and in good body condition. Three animals were euthanized and submitted for autopsy. Grossly, mink exhibited locally extensive to coalescing areas of crusting alopecia but no other significant gross lesions in internal organs. Microscopically, skin lesions were characterized by chronic hyperplastic dermatitis with folliculitis, furunculosis, occasional intracorneal pustules, and large numbers of intrafollicular fungal arthrospores and hyphae. The dermatophyte was cultured and identified as Trichophyton equinum based on molecular barcoding of the internal transcribed spacer region of the ribosomal DNA gene.

An automated data capture system comprising an electronic measuring board and digital balance linked to a microcomputer was developed for laboratory fish processing. The system conducts data plausibility tests on lengths, weights, gonad weights, and gonad maturity stages in real time, which allows errors to be corrected before the fish is discarded. In trials with samples of Atlantic herring Clupea harengus, the system detected data entry errors for 2.4% of fish; without the verification system, these errors would have entered data files. The automated system reduced processing time from 2.15 to 1.28 person-minutes per fish. Mean Atlantic herring length measurements made on electronic and hardwood boards differed by 0.6 mm, and measurements made by the two systems had similar standard deviations. Automated data capture systems increase the efficiency and accuracy of fish processing and have broad applications in fishery assessment programs.

Tissue samples and feces were collected from a dead, adult female coyote (Canis latrans) found at the side of the road in late March 2003 in the Avalon Peninsula region of Newfoundland, Canada. The coyote apparently died of vehicular-related trauma. Samples of lung, brain, heart, liver, and kidney were fixed in formalin and submitted for histologic examination. The entire remaining lung and heart also were submitted for examination. The coyote was diagnosed with moderate, multifocal, granulomatous interstitial pneumonia with eosinophilic vasculitis and many intralesional nematode eggs, larvae, and occasional intravascular adult worms. Adult nematodes recovered from the pulmonary arteries were identified as Angiostrongylus vasorum. Small foci of granulomatous inflammation, often containing nematode eggs and larvae, were scattered in the brain and kidney. To our knowledge, this is the first report of A. vasorum infection in a coyote from the only endemic area of infection in North America.

A large splenic mass was found during exploratory celiotomy in a 12-year-old Labrador retriever. Histologic examination of the mass revealed a mesenchymal chondrosarcoma. The dog was euthanatized 7 wk postoperatively, due to suspected tumor recurrence. Extraskeletal mesenchymal chondrosarcomas are rare in dogs and are associated with a guarded prognosis.

To test hexachlorobenzene (HCB), an environmental pollutant, for its potential toxicity to the ovary.Nonhuman primates were orally administered the pollutant at doses between 0.01 and 10 mg of HCB/kg of body weight to test viability of ovarian follicles. At the end of dosing period, the monkeys received a compound that contained follicle-stimulating and luteinizing hormones to stimulate development of follicles that would be examined by electron microscopy.Twenty, 6- to 13-year-old cynomolgus monkeys were randomly assigned to 5 groups.The HCB, in concentrations of 0.01, 0.1, 1.0, and 10.0 mg/kg, was orally administered with glucose in gelatin capsules for 13 weeks. Monkeys receiving capsules containing glucose only served as controls. After the 13th week, monkeys were given a compound that contained follicle-stimulating and luteinizing hormones daily during days 2 to 7 from the start of menses. On day 8 of the menstrual cycle, 5,000 IU of human chorionic gonadotropin was administered, and 35 to 38 hours later, 1 ovary from each monkey was obtained during laparotomy. Approximately 1-mm cubes of tissue from each ovary were harvested, fixed by immersion in buffered 2% glutaraldehyde, and processed for transmission electron microscopy.Ultrastructure of ovarian follicles was altered in the monkeys administered HCB. Lesions were observed in the follicles from monkeys given the lowest concentrations of HCB, and comprised condensed mitochondria in the developing ova and follicular cells that contained nuclei with deep indentations and abnormal accumulation of cytoplasmic lipid droplets. Alterations, such as herniation of the ooplasm, degeneration of the follicular cells, and appearance of abnormal spaces between follicular cells were observed in the follicles from monkeys of the 0.1 to 1.0 mg of HCB/kg dosage groups. The most relevant alterations were seen in the mitochondrion, an organelle that appeared to be most sensitive to the compound. Mitochondria were condensed, with abnormal intracristal spaces in the lower-dosage groups, and were markedly degenerated in the 10 mg/kg group. The effect of HCB were dose-related.The HCB is a reproductive system toxicant, and its damaging effects may be a result of augmentation of lipid peroxidation, especially in the primary follicle, which abnormally affects cellular membranes and thus, impairs their permeability.

"The Politics of Women's Education: Perspectives from Asia, Africa, and Latin America" reveals the complex changes in women's education throughout the world. Although women have made remarkable progress, their educational equity remains illusive and politically contested. This volume offer the first comprehensive assessment of what has been attempted, what remains to be done, and what are the options for reform. The book presents the authentic voice of third world women and men, relating their efforts to improve the position of women through education. It raises important questions for readers from both high- and low-income countriesabout whether formal or non- formal education will best serve women's needs; whether state or private initiatives are more likely to succeed in raising women's status through the delivery of transforming knowledge; and whether Western dreams of modernization have any relevance to non-Western societies. Countries covered include India, Pakistan, Korea, the Philippines, Kenya, Zimbabwe, Mexico, Peru, and Brazil. Each author locates women's education in the larger national context, thus unraveling the matrix that links gender and education to race, ethnicity, social class, and political change. The story is told directly, by women and men passionately committed to improvement through education. The book's immediacy will appeal to academic and general readers alike, and it will be a stimulus to thought and action forcitizens who wonder what's wr ong with the schools in their neighborhood. ." . . provides many insights into the lives, aspirations, achievements, and frustrations of women in Asia, Africa, and Latin America as they wrestle with issues of education and social change."--Journal of Asian Studies Jill Ker Conway is Visiting Scholar in the Program of Science, Technology and Society, MIT. Susan C. Bourque is Professor of Government, Smith College.

The twelve years of military rule in Peru between 1968 and 1980 witnessed few abuses of human rights, in marked contrast to the activities of military governments in Southern Cone countries like Brazil, Argentina, and Chile. Yet paradoxically, the return to democracy in Peru, with the election of Fernando Belaúnde in 1980 and Alan García in 1985, has brought sharp escalations in political violence and terror. Guerrilla activity by the Sendero Luminoso (“Shining Path”) in the highlands, urban terrorism, and a severe economic crisis have combined to pose a serious challenge to the authority of the state. Thus it is problematic to speak of a “return to democracy” while Peruvians are being subjected to expanding military control and repression.

A quasi-planar flow theory for the reattachment of annular jets has been developed. In addition to the hypotheses that are generally found in two-dimensional planar reattachments, it is supposed that everywhere along the path, the rate of entrainment of the jet is given by the planar flow theory providing that the origin (which is function of the distance along the path) is properly determined. Experimental measurements have been carried out with different geometries and the agreement with the theory is good.

SummaryAs part of a general investigation into Coanda effect, a study has been made of the reattachment of a two-dimensional, incompressible, turbulent jet to an adjacent, inclined, flat plate. The jet separates from the boundaries at the slot lips and reattaches to the plate downstream, a phenomenon which is associated with the lowering of the pressure between the jet and the plate accompanying the entrainment of fluid there. It is found that the flow becomes independent of both the length of the plate and the Reynolds number when these parameters are sufficiently large: the flow, scaled with respect to the width of the slot, is then uniquely determined by the plate inclination. Two approximate theories are developed for the mean pressure within the separation bubble, the position of reattachment and the increase in volume flow from the slot: the agreement with experiment is fairly satisfactory. These theories are a development of Dodds's analysis for the reattachment of a jet to a plate offset from, and parallel to, the axis of the slot and, for the purpose of comparison, a limited study is also made of this flow.

The reciprocal recurrent selection technique for improving combining ability was tested with two strains of chickens. Body weight of broilers was the criterion. Selection was based on the 10-week body weights of crossbred progeny but only selected individuals from the purebred strains were used as parents of succeeding generations. Random-bred control strains, in which no selection was practised, were also maintained and reproduced to assist in evaluating genetic change in the selected strains.Reciprocal recurrent selection brought about a progressive increase in the body weight of both purebred and crossbred broilers from the strains under selection. Broilers from the selected strains were 11.3 per cent heavier at 10 weeks of age than those from their unselected counterparts at the end of the experiment. Broilers from the control strains maintained a nearly constant weight. Purebred broilers from the selected strains increased in weight as the experiment progressed, at approximately the same rate as crossbreds from the same strains. Selection apparently resulted in an accumulation of genes having an additive favorable effect on body weight, but it failed to improve combining ability. Over-dominance and/or epistasis did not appear to be important factors in heterosis for body weight in these populations.

The effect of blockade of 5-hydroxytryptamine and norepinephrine uptake on voluntary ethanol consumption in rats was investigated. It was demonstrated that attenuation of ethanol intake occurred only as a result of treatment with specific 5-hydroxytryptamine uptake inhibitors. These results suggest that increasing the availability of central 5-hydroxytryptamine may in some way interfere with the positive reinforcing properties of ethanol. The second phase was designed to determine whether the attenuation of ethanol intake following blockade of 5-hydroxytryptamine uptake may be due to increased post-synaptic activity. Ethanol-preferring animals were pretreated with methergoline, a postsynaptic receptor blocker, followed by treatment with zimelidine, a 5-hydroxytryptamine uptake inhibitor. The results indicate that treatment with methergoline did not alter the zimelidineduced attenuation of ethanol intake. Based on these results it is suggested that blockade of 5-hydroxytryptamine uptake produces an attenuation of ethanol intake but not as a result of increased post-synaptic activity.

Male Wistar rats were presented with an everyday free choice between water and a morphine-sucrose solution. Following a 5-day baseline period animals were injected with either zimelidine (10 or 20 mg/kg, i.p.), a neuronal serotonin uptake inhibitor, or Ringer's solution (2 ml/kg, i.p.) for 5 consecutive days. Treatment with zimelidine was shown to significantly attenuate morphine drinking suggesting that an increased availability of serotonin may interfere with the positive reinforcing properties of morphine. The results are also discussed in terms of a possible interaction with brain norepinephrine. The possibility that the reinforcing effects of both morphine and ethanol are subserved by common mechanisms is suggested.

Studies women of two Peruvian highland communities and the cultural implications of gender differences