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Optimiser le renouvellement et les performances des ouvrages et réseaux qui maillent le territoire reste un enjeu majeur pour une gestion patrimoniale des infrastructures en pleine évolution. À travers l'analyse transversale de l'organisation des acteurs impliqués dans la gestion de différents types d'infrastructures – réseaux d'eau potable et d'assainissement, ouvrages hydrauliques, voiries locales et ouvrages d'art – l'article permet ici de mettre en évidence la grande diversité des configurations existantes et leurs implications en termes de gestion patrimoniale.

A number of freshwater fish particularly migratory species are currently under threat. As the pressure of recreational fishing has intensified, this activity needs to be more regulated to halt the decline of threatened fish stocks. This paper presents results of an economic valuation study of anglers' preferences for a change in the rules applying to Twaite shad fisheries in south-western France. Twaite shad has become a "near-threatened" species, with the same IUCN status as the Atlantic salmon. As shad recreational angling continues to be open access, new regulations are required to limit recreational harvest in support of a management strategy to prevent stock reduction even to foster stock recovery. We combined a deliberative choice experiment with open group discussion to obtain an in-depth understanding of anglers' preferences between two types of traditional regulatory instrument - a license fee or a bag limit. The closure of the fishery served as the reference scenario. The results indicate that the majority of anglers were aware of the decline of Twaite shad stocks. The recreational value of the species remains high, especially for those who already target migratory species. Although econometric estimations show that there are heterogeneous preferences across anglers, there were support for a change toward a bag limit, and an aversion for a high-priced licence fee. The findings also provided two key insights to improve recreational fishing management and preservation policy for threatened species. The communication of observation data on fish stock could not be considered as a sufficient ecological information to ensure threatened species monitoring leads to an effective co-management with anglers' community. Anglers' aversion to loss can be seen as a behaviour mechanism towards the preservation of the species rather than to its use., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Our digital age is characterized by both a generalized access to data and an increased call for participation of the public and other stakeholders and communities in policy design and decision-making. This context raises new challenges for political decision-makers and analysts in providing these actors with new means and moral duties for decision support, including in the area of environmental policy. The concept of "policy analytics" was introduced in 2013 as an attempt to develop a framework, tools, and methods to address these challenges. This conceptual initiative prompted numerous research teams to develop empirical applications of this framework and to reflect on their own decision-support practice at the science-policy interface in various environmental domains around the world. During a workshop in Paris in 2018, participants shared and discussed their experiences of these applications and practices. In this paper, we present and analyze a set of applications to identify a series of key properties that underpin a policy analytics approach, in order to provide the conceptual foundation for policy analytics to address current policy design and decision-making challenges. The induced properties are demand-orientedness, performativity, normative transparency, and data meaningfulness. We show how these properties materialized through these six case studies, and we explain why we consider them key to effective policy analytics applications, particularly in environmental policy design and decision-making on environmental issues. This clarification of the policy analytics concept eventually enables us to highlight research frontiers to further improve the concept.

In eukaryotes, the high-mobility-group (HMG) nuclear factors are highly conserved throughout evolution and are divided into three families, including HGMB, characterized by an HMG box domain. Some HMGB factors are DNA structure specific and preferentially interact with distorted DNA sequences, trigger DNA bending, and hence facilitate the binding of nucleoprotein complexes that in turn activate or repress transcription. In Plasmodium falciparum, two HMGB factors were predicted: PfHMGB1 and PfHMGB2. They are small proteins, under 100 amino acids long, encompassing a characteristic HMG box domain closely related to box B of metazoan factors, which comprises two HMG box domains, A and B, in tandem. Computational analyses supported the conclusion that the Plasmodium proteins were genuine architectural HMGB factors, and in vitro analyses performed with both recombinant proteins established that they were able to interact with distorted DNA structures and bend linear DNA with different affinities. These proteins were detected in both asexual- and gametocyte-stage cells in Western blotting experiments and mainly in the parasite nuclei. PfHMGB1 is preferentially expressed in asexual erythrocytic stages and PfHMGB2 in gametocytes, in good correlation with transcript levels of expression. Finally, immunofluorescence studies revealed differential subcellular localizations: both factors were observed in the nucleus of asexual- and sexual-stage cells, and PfHMGB2 was also detected in the cytoplasm of gametocytes. In conclusion, in light of differences in their levels of expression, subcellular localizations, and capacities for binding and bending DNA, these factors are likely to play nonredundant roles in transcriptional regulation of Plasmodium development in erythrocytes.

During the complex life cycle of Plasmodium falciparum, divided between mosquito and human hosts, the regulation of morphologic changes implies a fine control of transcriptional regulation. Transcriptional control, however, and in particular its molecular actors, transcription factors and regulatory motifs, are as yet poorly described in Plasmodium. In order to decipher the molecular mechanisms implicated in transcriptional regulation, a transcription factor belonging to the tryptophan cluster family was studied. In a previous work, the PfMyb1 protein, contained in nuclear extracts, was shown to have DNA binding activity and to interact specifically with myb regulatory elements. We used long pfmyb1 double-stranded RNA (dsRNA) to interfere with the cognate messenger expression. Parasite cultures treated with pfmyb1 dsRNA exhibited a 40% growth inhibition when compared with either untreated cultures or cultures treated with unrelated dsRNA, and parasite mortality occurred during trophozoite to schizont transition. In addition, the pfmyb1 transcript and protein decreased by as much as 80% in treated trophozoite cultures at the time of their maximum expression. The global effect of this partial loss of transcript and protein was investigated using a thematic DNA microarray encompassing genes involved in signal transduction, cell cycle and transcriptional regulation. SAM software enabled us to identify several genes that were differentially expressed and probably directly or indirectly under the control of PfMyb1. Using chromatin immuno-precipitation, we demonstrated that PfMyb1 binds, within the parasite nuclei, to several promoters and therefore participates directly in the transcriptional regulation of the corresponding genes. This study provides the first evidence of a regulation network involving a Plasmodium transcription factor.

During the complex life cycle of Plasmodium falciparum, through mosquito and human, the erythrocytic cycle is responsible for malarial disease and transmission. The regulation of events that occur during parasite development, such as proliferation and differentiation, implies a fine control of transcriptional activities that in turn governs the expression profiles of sets of genes. Pathways that underline gametocyte commitment are yet poorly understood even though kinases and transcription factors have been assumed to play a crucial role in this event. In order to understand the molecular mechanisms controlling the variation of gene expression profiles that might participate in early gametocytogenesis, the transcriptome of two clones, 3D7 and its gametocyte-less derivative F12, was compared at five time points of the erythrocytic asexual development. We have used a thematic DNA microarray containing 150 PCR fragments, representative of P. falciparum genes involved in signal transduction, cell cycle and transcriptional regulation. We identified several genes eliciting different expression profiles among which some implicated in gene regulation or encoding putative transcription factors. The differential expression of transcription factor and kinase transcripts observed in the two clones may enlighten genes that might have a role in impairment of the early gametocytogenesis of the F12 clone.