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Summary Hemoglobin C (HbC) is variant hemoglobin with a mutation in the betaglobin gene causing substitution of glutamic acid for lysine at position 6 of the globin chain. We are presenting in our observation a case of homozygous HbC in a young military of 27 years old, single, of Algerian origin. The biological examinations during a systematic checkup shows a mild hemolytic anemia (reticulocytes increased and collapsed haptoglobin), the morphological examination of the peripheral blood shows an aniso-poikilocytose, abundant target cells and occasionally an erythroblastosis. This patient has neither surgical nor medical history. The clinical examination reveals a moderate and non-painful isolated splenomegaly, inorganic cardiac murmur without other clinical signs associated. The presence of a hemoglobinopathy has been suggested by using an alkaline pH buffer electrophoresis system and confirmed by a high performance liquid chromatography (HPLC), which quantifies the various fractions of the hemoglobin (HbC: 92,6%, HbF: 1,6% and the HbA2: 3,7% of our patient) and allows to identify the homozygous or heterozygous character of the genetic abnormality. The investigated patient is homozygous CC. Electrophoresis hemoglobin at pH alkaline confuses the HbC with the HbE, HbA2, HbO-arab… This one is well-differentiated by the capillary electrophoresis and the HPLC. The screening of these asymptomatic hemoglobinopathy allows to detect the couples at risk and to propose a genetic counseling.

Resume La production de slime et les proprietes d'adherence ont ete etudiees chez 68 souches de staphylocoques coagulase negative (SCN) isolees d'hemocultures dont 57 Staphylococcus epidermidis, 7 S. haemolyticus, 3 S. hominis, 1 S. capitis. 43 de ces souches ont ete considerees comme pathogenes sur des criteres cliniques et bacteriologiques, 25 comme contaminantes. La production de slime a ete mise en evidence chez 34 des 43 souches pathogenes et seulement, chez 9 des 25 souches contaminantes (p = 0,006), l'adherence chez 35 des 43 souches pathogenes et chez 8 des 25 souches contaminantes (p = 0,001). Ces resultats montrent que dans notre etude la production de slime et l'adherence sont correlees a la pathogenicite des SCN et que ces tests simples peuvent aider a preciser le role pathogene de certains isolats.

Objectives: To assess the frequency and diversity of extended spectrum b-lactamases (ESBLs) in the Champagne-Ardenne region France, and to identify genetic elements associated with the blaCTX-M genes. Methods: During 2004, all the non-duplicate isolates of Pseudomonas aeruginosa and Acinetobacter baumannii resistant to ceftazidime and of Enterobacteriaceae intermediate or resistant to ceftazidime and/or cefotaxime, screening samples excluded, were collected in 10 public hospitals and 3 private clinics. bla genes were sequenced and blaCTX-M environment characterized by PCR mapping. Results: In Enterobacteriaceae (138/21 861; 0.6%), ESBLs were predominantly TEM-24 (n 5 52; 37.7%) and CTX-M-15 (n 5 37; 26.8%). Three new enzymes were identified, CTX-M-61 (CTX-M-1 group), TEMand SHV-type. A. baumannii (n 5 5) produced VEB-1 and P. aeruginosa (n 5 2) SHV-2a. ISEcp1 was detected in 22/27 strains, disrupted in 7 of them. The IS903-like element was downstream of blaCTX-M-14 and blaCTX-M-16 .I SCR1 was found upstream of blaCTX-M-2 and blaCTX-M-9, and ISCR1 and blaCTX-M-2 were located on a sul1-type class 1 integron. In comparison with 2001‐02, ESBL distribution among Enterobacteriaceae showed an increase in CTX-M-type (44.9% vs 3.7% P < 10 27 ) due to Escherichia coli CTX-M-15 and to the almost total disappearance of TEM-3 (0.9% vs 51.2%). E. coli was the most frequent species (50.0% vs 5.1% in 1998) despite a similar prevalence to that in 1998 (0.5% vs 0.2%). Conclusions: A careful detection of blaCTX-M-type spread to other species would help to anticipate clonal endemics such as those observed in Enterobacter aerogenes TEM-24.