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Context: Garlic (Allium sativum) with its main component organosulfur compounds has an anticancer effect against a large variety of cancer cells. In this study we examined the anti-cancer activity of garlic extract on KB- cancer cell lines. Aims: a) To determine the cytotoxic activity of garlic extract on KB cell line, b) To determine the cytotoxic activity of 5-FU on KB cell line, c) To compare the cytotoxic activity of garlic extract and 5 FU on KB cell line. Methods and materials: An extract of ascending concentration was prepared using 5-FU and garlic. The KB cell lines were treated with both these extracts. The cytotoxic properties of the extracts were assessed by MTT assay by measuring the number of viable cells. Results: The results showed that the percentage of viable cells is significantly correlated with different concentrations of garlic (Allium sativum) extract (p<0.05) and is significant with a p value of 0.0027. Conclusion: Epidemiological observations and studies have indicated the anti-carcinogenic potential of garlic, traditionally used for various human diseases. This study aims to utilize this potential of garlic or its components as an adjuvant to an anticancer drug or in the development of new anticancer drugs. [ABSTRACT FROM AUTHOR]

Introduction: Numerous studies have stated an association of spice consumption with lowered incidence of oral squamous cell carcinoma and other potentially malignant disorders of oral cavity. Anise seeds when consumed have the potential medicinal benefits including anticancer, anti-helminthic, hepato-protective and antioxidant activities. In this study we examined the anti-cancer activity of Anise seed extract (aqueous extract) on KB cancer cell lines. Aims: a) To determine the cytotoxic activity of Anise seed extract on KB cell line b) To determine the cytotoxic activity of CISPLATIN on KB cell line c) To compare the cytotoxic activity of Anise seed extract and CISPLATIN on KB cell line. Methods and Material: The study was conducted in Biogenix Research Centre, Thiruvananthapuram. The KB cancer cell lines were procured from NCCS, Pune. Anise seeds were purchased, dried and powdered using mortar and pestle. An extract was prepared in ascending concentrations and added to KB cell line in vitro under standardized environment. An extract of CISPLATIN at the same concentrations were prepared and added to KB cell line separately. Cytotoxic activity of the anise seed extract and CISPLATIN extract were assessed using MTT assay and cells were observed under an inverted phase contrast microscope, for any detectable changes in the cell morphology. Results: The results obtained showed that with ascending concentrations of anise seed extract there was significant cytotoxic changes observed with p value significant and obtained at 0.0052. Conclusion: Since anise seeds have been known to have a great potential as a chemotherapeutic agent, further focused studies of its anticancer properties and isolation of compounds from Pimpinella anisum are necessary to prove its worth in the cancer therapy. [ABSTRACT FROM AUTHOR]