1. Antagonistic cytoprotective effects of C60 fullerene nanoparticles in simultaneous exposure to benzo[a]pyrene in a molluscan animal model
- Author
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Volker M. Arlt, Aldo Viarengo, Yann Aminot, Awadhesh N. Jha, Susanna Sforzini, Andrei N. Khlobystov, Mohamed Banni, Michael N Moore, James W. Readman, Audrey Barranger, Plymouth University, University of Exeter Medical School, University of Exeter, Plymouth Marine Laboratory (PML), Plymouth Marine Laboratory, Consiglio Nazionale delle Ricerche (CNR), Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), University of Nottingham, UK (UON), King‘s College London, Institut Salah Azaiz [Tunis] (ISA), and Natural Environment Research Council (NERC), UK UK Research and Innovation (UKRI) NERC Natural Environment Research Council [NE/L006782/1]
- Subjects
Environmental Engineering ,animal structures ,010504 meteorology & atmospheric sciences ,Membrane permeability ,Cell ,mTORC1 ,Oxidative phosphorylation ,010501 environmental sciences ,01 natural sciences ,Lipofuscin ,chemistry.chemical_compound ,medicine ,polycyclic compounds ,Autophagy ,Environmental Chemistry ,Oxidative-injury ,Waste Management and Disposal ,0105 earth and related environmental sciences ,chemistry.chemical_classification ,Antagonism ,Reactive oxygen species ,Chemistry ,Complexity ,Pollution ,C-60-nanoparticles ,Cytosol ,medicine.anatomical_structure ,Biochemistry ,Benzo(a)pyrene ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,Lysosomes - Abstract
International audience; The hypothesis that C fullerene nanoparticles (C) exert an antagonistic interactive effect on the toxicity of benzo[a]pyrene (BaP) has been supported by this investigation. Mussels were exposed to BaP (5, 50 & 100μg/L) and C (C-1mg/L) separately and in combination. Both BaP and C were shown to co-localize in the secondary lysosomes of the hepatopancreatic digestive cells in the digestive gland where they reduced lysosomal membrane stability (LMS) or increased membrane permeability, while BaP also induced increased lysosomal lipid and lipofuscin, indicative of oxidative cell injury and autophagic dysfunction. Combinations of BaP and C showed antagonistic effects for lysosomal stability, mTORC1 (mechanistic target of rapamycin complex 1) inhibition and intralysosomal lipid (5 & 50μg/L BaP). The biomarker data (i.e., LMS, lysosomal lipidosis and lipofuscin accumulation; lysosomal/cell volume and dephosphorylation of mTORC1) were further analysed using multivariate statistics. Principal component and cluster analysis clearly indicated that BaP on its own was more injurious than in combination with C. Use of a network model that integrated the biomarker data for the cell pathophysiological processes, indicated that there were significant antagonistic interactions in network complexity (% connectance) at all BaP concentrations for the combined treatments. Loss of lysosomal membrane stability probably causes the release of intralysosomal iron and hydrolases into the cytosol, where iron can generate harmful reactive oxygen species (ROS). It was inferred that this adverse oxidative reaction induced by BaP was ameliorated in the combination treatments by the ROS scavenging property of intralysosomal C, thus limiting the injury to the lysosomal membrane; and reducing the oxidative damage in the cytosol and to the nuclear DNA. The ROS scavenging by C, in combination with enhanced autophagic turnover of damaged cell constituents, appeared to have a cytoprotective effect against the toxic reaction to BaP in the combined treatments.
- Published
- 2021