Search

Your search keyword '"Černá, S."' showing total 12 results
Start Over Author "Černá, S."
12 results on '"Černá, S."'

5. Inflammatory and cytotoxic effects as well as histological findings of selected industrial fibrous dusts in Fischer rats after intratracheal instillation

Author

Hurbánková, M., Tátrai, E., Černá, S., Six, É, Kováčiková, Z., and Soterios Kyrtopoulos

Subjects
GeneralLiterature_INTRODUCTORYANDSURVEY, InformationSystems_INFORMATIONSTORAGEANDRETRIEVAL, ComputingMethodologies_DOCUMENTANDTEXTPROCESSING, GeneralLiterature_REFERENCE(e.g.,dictionaries,encyclopedias,glossaries)
Abstract

Journal URL: http://www.springer.com/11756

Published
2008

6. Toxicity of indoor Stachybotrys chartarum

Author

Piecková, Hurbánková, M., Pivovarová, Z., Černá, S., Kováčiková, Z., Lišková, A., Tatrai, E., and Šegvić, Maja

Subjects
Stachybotrys chartarum, histology, haematology, citotoxicity
Abstract

The toxigenic mould Stachybotrys chartarum has been reported to cause animal and human toxicoses, including fatal ones of babies living in contaminated buildings. In vitro respiratory toxicity (ceasing of chick tracheal ciliary beating and activity of alkaline phosphatase in the rat lung epithelial type II cells in 3 d) as well as in vivo toxicity of the complex mixtures of chloroform-extractable metabolites of a S. chartarum strain – atranone chemotype (detected by thin layer chromatography) isolated from a mouldy office in Bratislava, SK, after the intratracheal instilation (4  g) in Wistar male rats (ca. 200 g) was evaluated. Histological changes (by autopsy and lectinhistochemistry), hematological parameters (leukocyte, erythrocyte and thrombocyte cell counts, hemoglobin and hematocrit), cytotoxic (phagocytic activity and viability of alveolar macrophages (AM), lactate dehydrogenase and acid phosphatase activities) and inflammatory response biomarkers (total cell counts, number of AM, granulocyte and AM differentials) were measured in blood or the bronchoalveolar lavage fluid (BALF) after 3 d. The exometabolites showed higher in vitro activity than endometabolites, especially when originated from the fungal growth on building materials (plasterboard). No significant inflammatory changes of lung tissue, but fragmentation and coarsing of T II lung cell memebrane were found. The exometabolites also proved to be erythrocyte suppressors and siderophores. They caused severe injury of upper and lower airways (cytotoxic – decreased AM viability, though no significant changes of phagocytic activity, and inflammation inducing – increased total cell counts, proportion of binuclear cells and of young monocytic AM form, while decreased AM count as well as its proportion in the BALF - activity), too. Anyway, risk assessment to humans requires further studies on dose – effect relationship, pulmonary deposition characteristics of fungal particles and chronic exposure.

Published
2004

12. Exposure to nanoparticles of magnetite Fe 3 O 4 in three different doses and their influence on selected respiratory parameters of bronchoalveolar lavage after intravenous instillation.

Author

Hurbánková M, Volkovová K, Černá S, Wimmerová S, and Moricová Š

Subjects
Rats, Animals, Rats, Wistar, Bronchoalveolar Lavage, Administration, Intravenous, Bronchoalveolar Lavage Fluid, Ferrosoferric Oxide, Nanoparticles
Abstract

Objectives: Due to nano-dimensions (less than 100 nm), can nanoparticles probably penetrate through various membranes and travel from the bloodstream to other organs in the body. The aim of our study was to find out whether NPs Fe 3 O 4 (coated with sodium oleate) injected into the tail vein of laboratory Wistar rats pass through the bloodstream to the respiratory tract (in comparison with a control group); and if so whether increasing doses of NPs Fe 3 O 4 have an escalating harmful effect on selected bronchoalveolar lavage (BAL) parameters., Methods: Wistar rats were intravenously given 3 doses of the suspension of NPs Fe 3 O 4 (0.1% LD50 = 0.0364, 1.0% = 0.364 and 10.0% = 3.64 mg/kg animal body weight). Seven days later, we sacrificed the animals under anaesthesia, performed bronchoalveolar lavage (BAL), and isolated the collected cells. Many inflammatory and cytotoxic BAL parameters were examined., Results: Both inflammatory and cytotoxic BAL parameters affected by Fe 3 O 4 suspension were changed compared to control results, but not all were statistically significant. Thus, the NPs Fe 3 O 4 passed through the bloodstream to the respiratory tract and affected it. The highest concentration of NPs Fe 3 O 4 (10%) had the most influence on BAL parameters (7 of 12 parameters). Only 3 parameters showed a pure dose dependence., Conclusion: We assume that the adverse effect of Fe 3 O 4 NPs in our study is probably not correlated with the dose, but rather with the size of the particles or with their surface area.

Published
2023
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results