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30 results on '"Byeon, Jin-Ju"'

11. Qualification and application of liquid chromatography-quadrupole time-of-flight mass spectrometric method for the determination of carisbamate in rat plasma and prediction of its human pharmacokinetics using physiologically based pharmacokinetic modeling

Author

Lee, Byeong ill, primary, Lim, Jeong-hyeon, additional, Park, Min-Ho, additional, Shin, Seok-Ho, additional, Byeon, Jin-Ju, additional, Choi, Jang-mi, additional, Park, Seo-jin, additional, Park, Min-jae, additional, Park, Yuri, additional, and Shin, Young G., additional

Published
2020
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13. Strategic approach to developing a self-microemulsifying drug delivery system to enhance antiplatelet activity and bioavailability of ticagrelor

Author

Na,Young-Guk, Byeon,Jin-Ju, Wang,Miao, Huh,Hyun Wook, Son,Gi-Ho, Jeon,Sung-Hoon, Bang,Ki-Hyun, Kim,Sung-Jin, Lee,Hye-Jin, Lee,Hong-Ki, Cho,Cheong-Weon, Na,Young-Guk, Byeon,Jin-Ju, Wang,Miao, Huh,Hyun Wook, Son,Gi-Ho, Jeon,Sung-Hoon, Bang,Ki-Hyun, Kim,Sung-Jin, Lee,Hye-Jin, Lee,Hong-Ki, and Cho,Cheong-Weon

Abstract

Young-Guk Na,1 Jin-Ju Byeon,1 Miao Wang,1 Hyun Wook Huh,1 Gi-Ho Son,1,2 Sung-Hoon Jeon,1,3 Ki-Hyun Bang,1,2 Sung-Jin Kim,1 Hye-Jin Lee,1 Hong-Ki Lee,1 Cheong-Weon Cho1 1College of Pharmacy, Chungnam National University, Daejeon, Republic of Korea; 2Korea United Pharmaceutical Co. Ltd., Sejong, Republic of Korea; 3SamA Pharmaceutical Co. Ltd., Suwon, Republic of Korea Background: Ticagrelor (TCG) is used to inhibit platelet aggregation in patients with acute coronary syndrome, but its poor solubility and low bioavailability limit its in vivo efficacy. The purpose of this study was to manufacture an optimized TCG-loaded self-microemulsifying drug delivery system (SMEDDS) to enhance the oral bioavailability and antiplatelet activity of TCG. Materials and methods: Solubility and emulsification tests were conducted to determine the most suitable oils, surfactants, and cosurfactants. Scheffé’s mixture design was applied to optimize the percentage of each component applied in the SMEDDS formulation to achieve optimal physical characteristics, ie, high solubility of TCG in SMEDDS, small droplet size, low precipitation, and high transmittance. Results: The optimized TCG-loaded SMEDDS (TCG-SM) formulation composed of 10.0% Capmul MCM (oil), 53.8% Cremophor EL (surfactant), and 36.2% Transcutol P (cosurfactant) significantly improving the dissolution of TCG in various media compared with TCG in Brilinta® (commercial product). TCG-SM exhibited higher cellular uptake and permeability in Caco-2 cells than raw TCG suspension. In pharmacokinetic studies in rats, TCG-SM exhibited higher oral bioavailability with 5.7 and 6.4 times higher area under the concentration–time curve and maximum plasma concentration, respectively, than a raw TCG suspension. Antiplatelet activity studies exhibited that the TCG-SM formulation showed significantly improved inhibition of platelet aggregation compared with raw TCG at the same dose of TCG. And, a 10 mg/kg do

Published
2019

20. Quantification and application of a liquid chromatography-tandem mass spectrometric method for the determination of WKYMVm peptide in rat using solid-phase extraction

Author

Byeong Ill Lee, Young G. Shin, Yuri Park, Jae Ho Kim, Byeon Jin Ju, Soon Chul Heo, Seok Ho Shin, and Min-Ho Park

Subjects
0301 basic medicine, Accuracy and precision, Calibration curve, Phenanthroline, Clinical Biochemistry, Peptide, 01 natural sciences, Biochemistry, Sensitivity and Specificity, Analytical Chemistry, Rats, Sprague-Dawley, 03 medical and health sciences, chemistry.chemical_compound, Pharmacokinetics, Tandem Mass Spectrometry, Drug Discovery, Animals, Sample preparation, Solid phase extraction, Molecular Biology, Pharmacology, chemistry.chemical_classification, Chromatography, Tandem, 010401 analytical chemistry, Solid Phase Extraction, Reproducibility of Results, General Medicine, 0104 chemical sciences, Rats, 030104 developmental biology, chemistry, Linear Models, Oligopeptides, Chromatography, Liquid
Abstract

A liquid chromatographic-electrospray ionization-time-of-flight/mass spectrometric (LC-ESI-TOF/MS) method was developed and applied for the determination of WKYMVm peptide in rat plasma to support preclinical pharmacokinetics studies. The method consisted of micro-elution solid phase extraction (SPE) for sample preparation and LC-ESI-TOF/MS in the positive ion mode for analysis. Phenanthroline (10mg/mL) was added to rat blood immediately for plasma preparation followed by addition of trace amount of 2M hydrogen chloride (HCl) to plasma before SPE for the stability of WKYMVm peptide. Then, sample preparation using micro-elution SPE was performed with verapamil as an internal standard. A quadratic regression (weighted 1/concentration2), with an equation y=ax2+bx+c, was used to fit calibration curves over the concentration range of 3.02~2200 ng/mL for WKYMVm peptide. The quantification run met the acceptance criteria of ±25 % accuracy and precision values. For quality control samples at 15, 165, and 1820 ng/mL from the quantification experiment, the within-run and the between-run accuracy ranged from 92.5 to 123.4 % with precision values ≤15.1 % for WKYMVm peptide from the nominal values. This novel LC-ESI-TOF/MS method was successfully applied to evaluate the pharmacokinetics of WKYMVm peptide in rat plasma.

Published
2017

22. A Liquid Chromatography-Quadrupole-Time-of-Flight Mass Spectrometric Assay for the Quantification of Fabry Disease Biomarker Globotriaosylceramide (GB3) in Fabry Model Mouse

Author

Shin, Seok-Ho, primary, Park, Min-Ho, additional, Byeon, Jin-Ju, additional, Lee, Byeong, additional, Park, Yuri, additional, Ko, Ah-ra, additional, Seong, Mi-ran, additional, Lee, Soyeon, additional, Kim, Mi, additional, Seo, Jinwook, additional, Jung, Myung, additional, Jin, Dong-Kyu, additional, and Shin, Young, additional

Published
2018
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28. Quantification and application of a liquid chromatography–tandem mass spectrometric method for the determination of WKYMVm peptide in rat using solid‐phase extraction.

Author

Lee, Byeong ill, Park, Min‐Ho, Heo, Soon Chul, Park, Yuri, Shin, Seok‐Ho, Byeon, Jin‐Ju, Kim, Jae Ho, and Shin, Young G.

Abstract

Abstract: A liquid chromatographic–electrospray ionization–time‐of‐flight/mass spectrometric (LC‐ESI‐TOF/MS) method was developed and applied for the determination of WKYMVm peptide in rat plasma to support preclinical pharmacokinetics studies. The method consisted of micro‐elution solid‐phase extraction (SPE) for sample preparation and LC‐ESI‐TOF/MS in the positive ion mode for analysis. Phenanthroline (10 mg/mL) was added to rat blood immediately for plasma preparation followed by addition of trace amount of 2  m hydrogen chloride to plasma before SPE for stability of WKYMVm peptide. Then sample preparation using micro‐elution SPE was performed with verapamil as an internal standard. A quadratic regression (weighted 1/concentration2), with the equation y = ax2 + bx + c was used to fit calibration curves over the concentration range of 3.02–2200 ng/mL for WKYMVm peptide. The quantification run met the acceptance criteria of ±25% accuracy and precision values. For quality control samples at 15, 165 and 1820 ng/mL from the quantification experiment, the within‐run and the between‐run accuracy ranged from 92.5 to 123.4% with precision values ≤15.1% for WKYMVm peptide from the nominal values. This novel LC‐ESI‐TOF/MS method was successfully applied to evaluate the pharmacokinetics of WKYMVm peptide in rat plasma. [ABSTRACT FROM AUTHOR]

Published
2018
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30. Pharmacokinetic and Metabolism Studies of Monomethyl Auristatin F via Liquid Chromatography-Quadrupole-Time-of-Flight Mass Spectrometry.

Author

Park, Min-Ho, Lee, Byeong ill, Byeon, Jin-Ju, Shin, Seok-Ho, Choi, Jangmi, Park, Yuri, Shin, Young G., Lei, Yu, Dong, Qiuchen, and Liu, Yixin

Subjects
MASS spectrometry, ANTIBODY-drug conjugates, TIME-of-flight mass spectrometry, METABOLISM, MATRIX effect, DEMETHYLATION
Abstract

A simple liquid chromatography–quadrupole-time-of-flight–mass spectrometric assay (LC-TOF-MS/MS) has been developed for the evaluation of metabolism and pharmacokinetic (PK) characteristics of monomethyl auristatin F (MMAF) in rat, which is being used as a payload for antibody-drug conjugates. LC-TOF-MS/MS method was qualified for the quantification of MMAF in rat plasma. The calibration curves were acceptable over the concentration range from 3.02 to 2200 ng/mL using quadratic regression. MMAF was stable in various conditions. There were no significant matrix effects between rat and other preclinical species. The PK studies showed that the bioavailability of MMAF was 0% with high clearance. Additionally, the metabolite profiling studies, in vitro/in vivo, were performed. Seven metabolites for MMAF were tentatively identified in liver microsome. The major metabolic pathway was demethylation, which was one of the metabolic pathways predicted by MedChem Designer. Therefore, these results will be helpful to understand the PK, catabolism, and metabolism behavior of MMAF comprehensively when developing antibody-drug conjugates (ADCs) in the future. [ABSTRACT FROM AUTHOR]

Published
2019
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