Search

New isatin-thiosemicarbazone (1–6) were obtained from various 5-methylisatin and various isothiocyanates with good yields and efficient methods. The structures of the synthesized compounds were clarified by FT-IR, 1H-NMR, and 13C-NMR spectroscopic techniques and elemental analysis. The antioxidant activity properties of the compounds were investigated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method and total reducing power determinations. In our total reducing power study, where we chose Trolox as the standard antioxidant, it was found that the reducing powers of compounds containing different substituent groups and positions were close to each other and lower than Trolox. In the study using butylated hydroxytoluene (BHT) as a reference antioxidant, DPPH antiradical scavenging capacities of the synthesized compounds were determined. The free radical scavenging effects of the synthesized compounds were compared with the IC50 values obtained from the concentration equations and were found in the order BHT > 6 > 3 > 5 > 1 > 2 > 4. Structural and electronic properties of the compounds were examined by density functional theory (DFT) calculations and their relationship with the antioxidant properties of the compounds was discussed. DFT calculations played an important role in determining how the electronic properties of the synthesized compounds, especially in the presence of different substituents and in the ortho and meta positions, and accordingly the antioxidant properties of the compounds, changed. Experimental and theoretical results confirmed that methoxy-containing structures in the synthesized compounds exhibit higher antioxidant effects compared to halogen-containing structures, regardless of their position. [ABSTRACT FROM AUTHOR]

BACKGROUND: The Cosmos sulphureus Cav. plant is studied for its high polyphenolic content with antioxidant properties. Its flowers, rich in phenolic acids, flavonoids, and tannins, hold promise as antioxidants in food preservation. The inclusion of these compounds in chickpea‐based coatings with a previously studied preservative effect would be an excellent option as a food preservation method and microencapsulation addresses challenges like dispersion and degradation of polyphenols in the coating. The objective of this research was to evaluate the in vitro antioxidant activity of Cosmos sulphureus leaves, seed, and flower extracts and explore the protective effects of chickpea‐based coatings containing microcapsules of flower polyphenolic extract on the chemical quality of stored roasted sunflower seeds during storage. RESULTS: The ethanolic leaf extract exhibited the highest antiradical activity, followed by the aqueous flower extract. After a storage period of 15 days, at 40 °C, the chickpea‐based coatings effectively delayed lipid oxidation in the roasted sunflowers seeds, and the inclusion of polyphenolic microcapsules with 0.01% extract (SMC 0.01%) in the coating significantly improved the protective effect. By day 15 of storage, SMC 0.01% showed comparable peroxide value, conjugated dienes, and linoleic acid content to samples containing the synthetic antioxidant BHT (butylated hydroxytoluene). Samples that only contained chickpea‐based coating and coating with polyphenolic microcapsules with 0.005% extract exhibited significantly greater reduction in fatty acid content compared to the 0.01% SMC treatment. Conclusion: The chickpea‐based coating with polyphenolic microcapsules demonstrated antioxidant activity akin to synthetic BHT, offering a promising biopackaging solution for lipid‐rich foods like roasted sunflower seeds. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

1. The present study assessed the effect of different antioxidants on the quality of chilled/frozen-thawed sperm of red-legged partridge.2. Sperm samples from 40 red-legged partridges were collected and extended 1:1 (v:v) with Lake and Ravie 84, supplemented with ascorbic acid or butylated hydroxytoluene (BHT) at 0, 0.2, 0.4, 0.8 mM and catalase (CAT) or superoxide dismutase (SOD) at 0, 100, 200 and 300 IU/ml. Ten sperm samples were used per concentration. Motility and viability were evaluated in fresh and after 6 h of chilling at 5°C or after freezing-thawing.3. For chilled sperm, the presence of ascorbic acid decreased viability and several motility variables; BHT 0.8 mM increased non-progressive motility (NPM, 26.7 ± 1.99 vs. 20.7 ± 2.12); CAT 200 IU/ml improved the rectilinear velocity (40.4 ± 4.63 μ/s vs. 29.9 ± 4.62 μ/s) and linear progression ratio (52.8 ± 3.11% vs. 45.4 ± 2.98%); SOD 100 IU/ml increased NPM (24.5 ± 1.21% vs. 19.3 ± 1.75%) and tended to improve total progressive motility (42.7 ± 3.33% vs. 33.2 ± 3.26%, p = 0.07). Using an extender supplemented with CAT 200 or SOD 100 did not improve the post-thawed sperm quality.4. The present work provides an advance in the optimisation of chilling and freezing protocols for red-legged partridge sperm. [ABSTRACT FROM AUTHOR]

Arum maculatum, cuckoo‐pint in folk linguistics, is a poisonous but medicinal plant with tremendous properties that need investigation. This study subjected cuckoo‐pint's parts to in vitro antioxidant, enzyme inhibition, and antimicrobial susceptibility testing to investigate its biological activity. Further, phytochemistry was analyzed by both LC–MS/MS and total phenol, flavonoid, saponin, and reducing sugar content determination assays. Antioxidant activities were determined by Fe3+, Cu2+, Fe3+‐TPTZ metal‐reducing ability, 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) and 2,2ʹ‐azino‐bis‐3‐ethylbenzothiazoline‐6‐sulfonic acid (ABTS) radical scavenging assays. According to the results, seeds and berries have remarkable metal‐reducing and radical‐scavenging effects as natural and synthetic analogs such as ascorbic acid, butylated hydroxyanisole, butylated hydroxytoluene, trolox, and α‐tocopherol. Inhibition studies over human carbonic anhydrase II (hCA II), acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and α‐amylase were performed, and IC50 values were determined for each enzyme inhibition. According to the results, the cuckoo‐pint seeds inhibited the AChE, BChE, and hCA II activity with greater IC50 values than reference inhibitors. Antimicrobial activity over pan‐resistant strains was achieved for each strain with the extracts, at least 20 mm zone diameter was obtained with the lowest concentrations. LC–MS/MS results revealed 24 bioactive metabolites associated with the extracts' biological activity. Notably, quinic acid and epicatechin correlated with cardioprotective, neuroprotective, antioxidant, and antidiabetic activity. [ABSTRACT FROM AUTHOR]

In this work, the stems, leaves, and flowers of Ruta chalepensis L., R. graveolens L., and R. montana L. harvested in Morocco were subject to a comparative study to determine the phytochemical composition, antioxidant capacities, and the content of total phenols and flavonoids. For this purpose, 45 crude extracts (CEs) were prepared using different solvents, including water, ethyl acetate, methanol, ethanol, and hexane. Upon undergoing secondary metabolites screening, it was discovered that the different parts of three Ruta species contain a high level of secondary metabolites such as alkaloids, catechic tannins, flavonoids, coumarins, sterols, and triterpenes. This was confirmed by the results of the quantitative analysis, which proved that the three Ruta species contain a significant amount of total phenolic compounds, ranging from 27.05 to 213.42 mg GAE/g CE. With regard to antioxidant capacities, antiradical power (ARP) results prove that the majority of CEs have great ARP (were within the range of 0.42–1.99) compared to the BHT (butylated hydroxytoluene) standard (0.33 APR). In addition, the results of total antioxidant capacity (TAC) analysis of Ruta CEs showed good TAC (varies between 37.67 and 396.80 mg AAE/g CE). [ABSTRACT FROM AUTHOR]

BACKGROUND: Root‐knot nematodes (RKNs), Meloidogyne spp., are one of the most destructive polyphagous plant‐parasitic nematodes. They pose a serious threat to global food security and are difficult to control. Entomopathogenic nematodes (EPNs) show promise in controlling RKNs. However, it remains unclear whether the volatile organic compounds (VOCs) emitted from EPN‐infected cadavers can control RKNs. RESULTS: We investigated the fumigation activity of VOCs released from cadavers infected by five different species of EPNs on RKNs in Petri dishes, and found that VOCs released from Steinernema feltiae (SN strain) and S. carpocapsae (All strain) infected cadavers had a significant lethal effect on second‐stage juveniles (J2s) of Meloidogyne incognita. The VOCs released from the cadavers infected with S. feltiae were analyzed using SPME‐GC/MS. Dimethyl disulfide (DMDS), tetradecane, pentadecane, and butylated hydroxytoluene (BHT), were selected for a validation experiment with pure compounds. The DMDS compound had significant nematicidal activity and repelled J2s. DMDS also inhibited egg hatching and the invasion of tomato roots by J2s. In a pot experiment, the addition of S. feltiae‐infected cadavers and cadavers wrapped with a 400‐mesh nylon net also significantly reduced the population of RKNs in tomato roots after 7 days. The number of root knots and eggs was reduced by 58% and 74.34%, respectively, compared to the control. CONCLUSION: These results suggested that the VOCs emitted by the EPN‐infected cadavers affected various developmental stages of M. incognita and thus have the potential to be used in controlling RKNs through multiple methods. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Objectives: The current study aimed to conduct a phytochemical screening of commonly known fruit red grape (Vitis vinifera L.) seed methanolic extract through gas chromatography and mass spectrometry (GC-MS) to identify the bioactive compounds responsible for its health benefits and evaluate the pharmacological potentialities of the extract and its fractions against oxidation, inflammation, pain, and diarrhea. Methods: The in vitro antioxidant, anti-inflammatory, and cytotoxic characteristics of methanolic extracts and various solvent fractions of V. vinifera were evaluated using the DPPH free radical scavenging assay, membrane stabilizing, and brine shrimp lethality bioassay. Furthermore, the study assessed the effects of crude extracts (200, 400, and 600 mg/kg of body weight) on pain relief and reduction of diarrhea in animals using methods such as tail immersion, the acetic acid-induced writhing technique, and a diarrheal mouse model induced with castor oil. Results: A total of 73 phytoconstituents were predominantly found in the seed extract based on the GC-MS analysis. Among the identified compounds, 9-octadecenamide (13.7%), and (9E,11E)-octadeca-9,11-dienoate (11.07%) are most abundant. Several notable constituents, such as gamma-sitosterol, stigmasterol, paromomycin, 4,6-cholestadienol, gamma-tocotrienol, 24-Propylidenecholest-5-en-3beta-ol, and alpha-tocopherol acetate, are also present. The methanolic extract of V. vinifera seed and its different solvent fractions showed promising antioxidant properties (IC50 = 1.19-17.42 µg/mL) compared to the standard antioxidant butylated hydroxytoluene (IC50 = 20.46 µg/mL). Aqueous soluble fraction exerted inhibition of nearly 50% heat-induced hemolysis compared to the standard acetylsalicylic acid (42%). Besides, all the tested doses (200, 400, and 600 mg/kg bw) of the crude extract showed significant (P <.05) analgesic and antidiarrheal effects. Conclusion: The current findings endorsed the health benefits of V. vinifera by revealing potent antioxidant, anti-inflammatory, analgesic, and antidiarrheal effects. Nevertheless, further in-depth analysis of the plant's chemical constituents and pharmacological effects on health is warranted for novel drug discovery from V. vinifera. [ABSTRACT FROM AUTHOR]

Summary This work aimed to determine the total anthocyanin and phenolic contents and antioxidant capacity of extracts of the black rice's cold, hot and ultrasonic‐assisted extracts and their preservative effect on the storage quality of beef patties. The three extracts exhibited high anthocyanin, phenolic content and antioxidant capacity, and they were used in beef patties at five treatments (negative control without extract, positive control with 0.01% butylated hydroxytoluene (BHT), 7.5% cold extract, 7.5% hot extract and 7.5% ultrasound‐assisted extract). Raw and cooked beef patties were tested at 0, 3, 6, 9 and 12 days of cold storage in aerobically packaged polyethylene containers regarding pH, oxidative stability, antioxidant capacity and microbial quality. While adding black rice extracts did not influence the proximate composition of raw samples (P > 0.05), it slowed down the pH and lipid oxidation during storage and provided pH and thiobarbituric acid reactive substances (TBARS) stability in all final products. The BHT and treatments with extract indicated a higher 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) value than the negative control. Also, the incorporation of black rice extracts slightly retarded bacterial growth. These results suggest that the black rice extracts at 7.5% can be used effectively to protect beef patties from microbial spoilage and oxidation during cold storage for 12 days. [ABSTRACT FROM AUTHOR]

The Sunda porcupine (Hystrix javanica, F.Cuvier, 1823) is a rodent-mammal species native to Indonesia and is utilised in traditional medicine for the treatment of various ailments. Some ethnic communities in Indonesia have traditional beliefs regarding Sunda porcupine's quills, which are thought to relieve back pain and toothache. Despite this traditional knowledge, there is limited scientific research on the topic. The aim of this study was to identify active compound in an ethanolic crude extract of Sunda porcupine's quills, and to evaluate its antioxidant and antimicrobial properties. The antioxidant activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH)-free radical scavenging assay while the antimicrobial activity was evaluated through microdilution resazurin assay. The total phenolic and flavonoid contents were also determined to support the antioxidant properties. The active compounds were identified using gas chromatography-mass spectrophotometer (GCMS) with the National Institute of Standards and Technology (NIST-11) library. The result showed that the extract possesses antioxidant properties (IC50 138.93 µg/mL) and antimicrobial properties against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Bacillus subtilis (B. subtilis), Pseudomonas aeruginosa (P. aeruginosa) and Candida albicans (C. albicans) (IC50 range 0.40 mg/mL-33.05 mg/mL). Total phenolic content (TPC) and total flavonoid content (TFC) were 27.29 ± 2.20 mgGAE/g and 27.09 ± 1.66 mgQE/g, respectively. A total of 24 active compounds from the crude extract were identified. As much as five compounds serve as antioxidant agents, including: butylated hydroxytoluene; eicosane; 1-iodo-hexadecane; methyl ester hexadecanoic acid; and L-(+)-ascorbic acid 2,6-dihexadecanoate. Furthermore, as much as 11 compounds serve as antimicrobial agents, including: tetradecane; pentadecane; 2-isopropyl-5-methyl-1-heptanol; hexadecane; butylated hydroxytoluene; eicosane; 1-iodo-hexadecane; methyl ester hexadecanoic acid; benzenepropanoic acid, 3,5-bis(1,1-dimethylethyl)-4-hydroxy-, methyl ester; L-(+)-ascorbic acid 2,6- dihexadecanoate; and octadecanoic acid. This study provides scientific validation for the use of the Sunda porcupine's quills in traditional medicine and highlights the potential for further research in animal bioprospecting. [ABSTRACT FROM AUTHOR]

The current study aims to comprehend the effect of the various combinations of 3,4‐dihydroxyphenylacetic acid (3,4‐DHPA) and its esters in varying molar ratios on retarding oxidation in bulk refined fish oil in the presence of 0.3% w/w water. All the esters and 3,4‐DHPA exhibited better performance than the synthetic antioxidant butylated hydroxytoluene. In the presence of trace water, the parent compound was found to have a synergistic effect with hexyl ester when used at a molar ratio of 2:1, respectively, exhibiting a higher percent reduction among all the esters and their combinations. The study also showed that the presence of extraneous water can significantly accelerate the oxidation (twofold) in a bulk fish oil system. This suggests that the microenvironment of the oil is altered when water is present and that association colloids play a key role in enhancing the rate of oxidation. Thus, while designing a competent mixture of antioxidants, the specific site of oxidation involved and the surface‐active components should be taken into consideration to efficiently retard oxidation. Practical Applications: Analyzing the impact of trace amounts of water on the efficiency of natural antioxidants, including 3,4‐DHPA and its lipophilic esters, helps to comprehend the required optimal antioxidant concentrations for such a dynamic bulk oil system. The study finds application in controlling oxidation in PUFA‐rich oils containing surface‐active minor components that can accelerate oxidation. The results of the study show that 3,4‐DHPA and its esters could be a potential replacement for the synthetic antioxidant BHT in all aspects. [ABSTRACT FROM AUTHOR]

Burn injuries worldwide pose significant health risks due to frequent microbial infections, which worsen complications and increase mortality rates. The conventional antimicrobial formulations are available in the form of ointments and creams. These formulations are very greasy and stick to the clothes. The applications of these formulations by finger or applicator produce pain in the affected area and incur the possibility of microbial infection. To overcome these hurdles, authors developed a novel non-propellent foam (NPF) based formulation containing chlorhexidine for effective topical delivery. Initially, NPF containing Labrasol® (26.7%), sodium lauryl sulfate (1.2%), hydroxy propyl methyl cellulose (0.56%), butylated hydroxytoluene (0.1%), ethanol (1%), and distilled water was prepared and assessed for its consistency, and ability to form foam. The NPF was statistically optimized using the Box-Behnken design to determine the effect of polymer and surfactants on the critical foam properties. The optimized formulation showed a collapse time of 45 s with a unique nature of collapsing upon slight touch which is highly beneficial for burn patients with microbial infection. The diffusion study showed that more than 90% of the drug was released within 6 h. The skin permeation study showed that 23% of the total drug permeated through the skin after 6 h with 7.64 µg/cm2/h permeation flux. The developed formulation showed good antibacterial activity. The minimum inhibitory concentration of prepared NPF was found to be 2.5 µg/mL, 2.5 µg/mL, and 5.0 µg/mL against E. coli (MTCC-1687), P. aeruginosa (MTCC-1688), and S aureus (MTCC-737) respectively. The developed NPF formulation showed quick collapse time, excellent spreadability, good anti-bacterial activity, and a non-sticky nature representing a promising avenue for burn wound treatment without using any applicator. [Display omitted] [ABSTRACT FROM AUTHOR]

The Gaga chicken is an indigenous Indonesian breed that is important to preserve using semen cryopreservation technology. The study was conducted to determine the effect of adding sorbitol and butylated hydroxytoluene (BHT) in the diluent on the structural and surface ultrastructure of cryopreserved Gaga chicken sperm during cryopreservation /frozen storage. The study aimed to assess how adding sorbitol and butylated hydroxytoluene (BHT) to the diluent affects the structure and surface ultrastructure of cryopreserved Gaga chicken sperm. A completely randomized design was employed with four treatments and 10 replications including egg yolk-lactate ringer diluent (EYLR) as the control group, EYLR diluent with 3 mM BHT, EYLR diluent with 2% sorbitol, and EYLR diluent with both 3 mM BHT and 2% sorbitol. Semen was collected using a massage technique from 4 male chickens aged approximately 10 months, pooled semen was diluted, packaged in 0.25 mL straws, equilibrated for 2 hours at 5 °C, pre-freeze for 10 minutes, frozen for 24 hours, and thawed for 30 seconds at 37 °C. The parameters evaluated were sperm plasma membrane integrity, acrosome integrity, DNA damage, mitochondrial functionality, and surface ultrastructure. The results showed that the treatment had a significant effect on plasma membrane integrity and post-thawing mitochondrial functionality compared to the control, but no effect was observed on acrosome integrity or DNA damage. The results showed that the combination treatment of BHT with sorbitol had a significant effect on plasma membrane integrity and post-thawing mitochondrial function, but did not affect acrosome integrity or DNA damage when compared to the control group. Ultrastructural observations indicated that cryopreservation caused damage to the head, middle, and tail of the sperm in the control groups. However, these changes were prevented by the diluent containing a combination of BHT and sorbitol. The addition of both components (BHT 3 mM + sorbitol 2%) effectively maintained plasma membrane integrity, mitochondrial functionality, and surface ultrastructure of Gaga chicken sperm during cryopreservation. [ABSTRACT FROM AUTHOR]

Under atmospheric pressure, KOH or HCl solution was added to the butylated hydroxytoluene (BHT) oxidation system to adjust the pH, and the oxidative consumption of BHT was monitored by high performance liquid chromatography (HPLC). To investigate the oxidation properties of BHT under pressurized conditions, the temperature and pressure changes during the oxidation process were monitored using the mini-closed pressure vessel test (MCPVT). The oxidation products of BHT were characterized after crystallization and purification and the possible oxidation pathway was speculated. The results showed that under atmospheric pressure, the degradation rate of BHT was less than 5% when the pH was 4 or 6, indicating good stability. At pH 10, the degradation rate of BHT was up to 59% and the oxidation reaction was rapid. KOH and HCl decreased the initial oxidation temperature of BHT from 133.1 ℃ to 53.0 ℃ and 49.1 ℃ in a pressurized closed container, respectively. 2, 6-Di-tert-butyl-4-hydroperoxy-4-methyl-2, 5-cyclohexanedione and 2, 6-di-tert-butyl-4-methyl-4-(3, 5-di-tert-butyl-4-hydroxyphenyl)-2, 5-cyclohexadien-1-one were produced during the oxidation of BHT in both atmospheric alkaline and pressurized environments. The experimental results suggested that BHT was susceptible to oxidative degradation in alkaline and pressurized environments, producing toxic products, posing safety risks.

The effects of the antioxidant, butylated hydroxytoluene (BHT), and the metal chelator, ethylenediaminetetraacetic acid (EDTA) in combination with cedarwood oil (CWO) were investigated for wood preservation against subterranean termites as well as two species of white-rot decay fungi and two species of brown-rot decay fungi. Vacuum pressure impregnation was used to treat wood blocks. Resistance of the treated wood test blocks was evaluated using a no-choice bioassay for termites and a soil bottle assay wood decay fungi. Eight treatments were tested: H2O only; BHT only; EDTA only; BHT with EDTA; CWO only; CWO with EDTA; CWO with BHT; and CWO with BHT plus EDTA. For termites, the lowest percentage wood mass losses were for the EDTA, BHT, CWO, and CWO/EDTA treatments, all of which were statistically equivalent. Correspondingly, these treatments all had the highest termite mortalities at 100%. The four species of decay fungi were affected differently by the wood treatments; however, overall CWO and EDTA gave the best protection against wood mass loss. The addition of BHT did not decrease mass loss.

BACKGROUND: Foods contain lipids that are easily susceptible to oxidation, which can modify their sensory properties. Although these compounds provide characteristic flavours and odours, there are also unwanted compounds, such as volatile secondary oxidation products, representing a recurring problem for both the industry and consumers. Synthetic antioxidants are often employed to prevent this but their chronic consumption can be detrimental to human health. The present study evaluates the antioxidant potential of ethanolic extracts from Ganoderma resinaceum and Phlebopus bruchii using an accelerated oxidation test. RESULTS: The composition profile of the extracts was investigated, identifying the presence of tryptophan, quinic acid, caffeic acid and 3,4‐dihydroxyphenylglycol‐phenolic acid. The antioxidant capacity of the extracts was compared with that of butylated hydroxytoluene (BHT) in sunflower oil that was oven‐heated at 60 °C. Chemical (peroxide value, p‐anisidine value and conjugated dienes) and volatile (2‐octenal, 2‐heptenal and 2,4‐decadienal) indicators were measured over 28 days. The peroxide value decreased for both extracts at a similar level to that of BHT 0.02% w/w, and conjugate dienes decreased in the presence of G. resinaceum 0.1% w/w. Meanwhile, p‐anisidine exhibited a slightly greater decrease for P. bruchii 0.1% w/w than for BHT. The sample with 0.1% w/w of extracts showed a reduction in volatile secondary oxidation compounds, indicating significant antioxidant activity. CONCLUSION: Based on these results, both extracts could be proposed as potential antioxidants in foods with a high lipid content. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

ABSTRACT By using a multicomponent reaction involving isatins, diamines or aminoalcohols, activated acetylenic compounds, haloketones, and ammonium acetate in aqueous media in the presence of Cu@Fe3O4/SiO2@MWCNTs as new catalyst, the novel pyridopyrroloindole was produced in high yields. This catalyst was produced by employing Petasites hybridus rhizome water extract as a green media and moderate base. The pyridopyrroloindole derivatives were newly synthesized compounds, and their antioxidant properties were assessed using the 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) radical trapping and ferric reducing ability of plasma (FRAP) experiments. The pyridopyrroloindole 6b showed higher performance than butylated hydroxytoluene (BHT) and tert‐butylhydroquinone (TBHQ) better results. This method is used to make pyridopyrroloindole derivatives, and it has the advantages of a quick reaction, high yields, and the ability to separate the products easily. [ABSTRACT FROM AUTHOR]

This study sought to investigate techniques for lowering diesel engine emissions by using waste seed as an alternative energy source in place of conventional fossil fuels. This study thoroughly investigated the process of transesterifying waste seed oil obtained from Bauhinia purpurea linn for use as fuel in a common rail direct injection (CRDI) diesel engine. Butylated hydroxytoluene (BHT) nanoparticles serve as a combustion enhancer. The engine outcome metrics were analyzed using diesel, Bauhinia purpurea linn biodiesel (BPLB) and BPLB-BHT blends (BPLB + 10μm BHT 10 ppm and BPLB + 20μm BHT 10 ppm) without changing the operating circumstances. At full brake power, the BPLB + 10μm BHT 10 ppm mix outperformed the BPLB and BPLB + 20μm BHT 10 ppm blends, but fell short of diesel. The BPLB + 10μm BHT 10 ppm blend reduced smoke opacity by 39.14%, hydrocarbon (HC) emissions by 42.71%, carbon monoxide (CO) emissions by 59.03% and oxides of nitrogen (NOx) emissions by 12.29% compared to neat diesel fuel at maximum brake power. [ABSTRACT FROM AUTHOR]

This study aimed to develop a high-quality dry emulsion incorporating omega-3, 6, and 9 fatty acid-rich ostrich oil for use as a dietary supplement. Extracted from abdominal adipose tissues using a low-temperature wet rendering method, the ostrich oil exhibited antioxidant properties, favorable physicochemical properties, microbial counts, heavy metal levels, and fatty acid compositions, positioning it as a suitable candidate for an oil-in-water emulsion and subsequent formulation as a dry emulsion. Lecithin was employed as the emulsifier due to its safety and health benefits. The resulting emulsion, comprising 10% w/w lecithin and 10% w/w ostrich oil, was stable, with a droplet size of 3.93 ± 0.11 μm. This liquid emulsion underwent transformation into a dry emulsion to preserve the physicochemical stability of ostrich oil, utilizing Avicel® PH-101 or Aerosil® 200 through a granulation process. Although Aerosil® 200 exhibited superior adsorption, Avicel® PH-101 granules surpassed it in releasing the ostrich oil emulsion. Consequently, Avicel® PH-101 was selected as the preferred adsorbent for formulating the ostrich oil dry emulsion. The dry emulsion, encapsulated with a disintegration time of 3.11 ± 0.14 min for ease of swallowing, maintained microbial loads and heavy metal contents within acceptable limits. Presented as granules containing butylated hydroxytoluene, the dry emulsion showcased robust temperature stability, suggesting the potential incorporation of animal fat into dry emulsions as a promising dietary supplement. [ABSTRACT FROM AUTHOR]

Background: Rumex vesicarius is a wild leafy plant belonging to the family Polygonaceae, renowned for its therapeutic benefits. The genus Rumex comprises approximately 150 species distributed globally. Objective: The study aimed to investigate the biological activities of R. vesicarius using in vitro and in silico methods. Methods: Rumex vesicarius was collected from the mountains in Hail and extracted with methanol. The phytochemical composition was qualitatively determined using colorimetric detection methods. Additional analyses included elemental analysis, in silico docking, antioxidant, antibacterial, and anti-biofilm properties. Results: The extract contained various classes of phytochemicals, including flavonoids, phenolics, tannins, terpenes, and saponins. Sixteen constituents were identified through molecular docking, revealing inhibition against the filamentous temperature-sensitive protein Z (FtsZ), a crucial factor in bacterial cell division. Six compounds exhibited low binding scores ranging from -8.3 to -5.0 kcal/mol, indicating efficient interaction at the active site. Elemental analysis identified 15 elements, with potassium being the most abundant, followed by calcium, aluminum, silicon, iron, phosphorus, sulfur, magnesium, titanium, strontium, zinc, manganese, bromine, and chromium. Antioxidant analysis revealed significant properties at lower concentrations compared to ascorbic acid, butylated hydroxytoluene, and b-carotene. Antibacterial analysis demonstrated inhibitory effects on Bacillus subtilis MTCC121 and Pseudomonas aeruginosa MTCC 741, with inhibition zones of 13.67 ± 1.0 mm and 11.50 ± 1.0 mm, respectively. The MIC and MBC values ranged from 250 to 500 mg/mL. R. vesicarius also exhibited anti-biofilm activity. Conclusion: Wild-grown R. vesicarius from the mountains of Hail is rich in bioactive phytochemicals and essential minerals, exhibiting notable antioxidant and antibacterial properties. [ABSTRACT FROM AUTHOR]

Purpose: This study aims to assess the oxidative stability of avocado oil (AO) at various temperatures, using butylated hydroxytoluene (BHT) as an artificial antioxidant and different concentrations of ultrasonic extract of Chlorella vulgaris. Design/methodology/approach: Extracts of C. vulgaris were obtained using four solvents: water, acetone, ethanol and 80% ethanol-aqueous. Standard techniques were used to conduct qualitative phytochemical screening of the extracts. The extracted samples were analyzed for total phenolics, total flavonoids, antioxidant activity and phenolic compound fractionation. Some physicochemical parameters of AO treated with various concentrations of C. vulgaris ultrasonic extract compared to a 200 ppm BHT and exposed to different temperatures were measured. Findings: The highest phenolic, flavonoids content and antioxidant activity was achieved by 80% ethanolic extract of C. vulgaris. The results showed that exposure of AO to high temperatures led to significant changes in the oil's physicochemical properties. These changes increased as the temperature increased. On the other hand, adding 80% ethanolic extract of C. vulgaris into AO reduced the effect of heat treatment on the change in physicochemical properties. Originality/value: Adding 80% ethanolic extract of C. vulgaris into AO can potentially reduce the impact of heat treatment on the alteration of physicochemical properties. [ABSTRACT FROM AUTHOR]

The objective of the research was to examine microbial characteristics, metabolites produced, and the potential radiological risks present in mining soils located in Keana, North Central Nigeria. Soil samples were collected from various locations within Keana, Nasarawa State. Bacterial isolation was carried out, and molecular techniques were employed to characterize the bacteria found in the collected soil samples. Additionally, the susceptibility of these isolates to antibiotics was determined, and the bacteria screened for their ability to produce metabolites. The isolated bacteria were classified into three groups: Actinobacteria, Firmicutes, and Proteobacteria. The analysis of the spectra revealed that 1595 compounds were produced, including carboxylic acids, nitro compounds, aldehydes, anhydrides, esters, ketones, amides, phenols, alcohols, alkanes, alkenes, alkynes, and arenes. Some of the metabolites produced were oleic acid, 1,3-dioxolane, linoelaidic acid and oleic acid, 1-nonadecene, butylated hydroxytoluene, diisooctyl phthalate, bis(2-ethylhexyl) phthalate among others, and 1,2-benzenedicarboxylic acid (85.32%) as the most produced metabolite. Among the antibiotics tested, levofloxacin and ciprofloxacin exhibited the strongest antibacterial properties against the isolates. Airborne gamma-ray spectrometry analysis identified elevated levels of potassium, thorium, and uranium in the soils, indicating potential environmental hazards. However, no significant correlation was found between the presence of bacteria and radioactive elements. These findings emphasize the importance of comprehensive environmental monitoring in Keana to address potential health risks associated with microbial contamination and radioactive materials. Additionally, the study highlighted the role of microbial diversity in Keana soils in promoting the production of secondary metabolites with potential applications in pharmaceutical and industrial sectors.. [ABSTRACT FROM AUTHOR]

The antioxidant properties of 3-dithiocarbamic flavanones have been investigated. The influence of the halogen substituents on ring A of the flavanones and the nature of the secondary amine from the dithiocarbamic moiety have been accounted. The results indicated that the presence of a halogen substituent at the C-8 position of the benzopyran ring induce better antioxidant properties against DPPH and ABTS than butylated hydroxytoluene (BHT) and ascorbic acid. The presence of a halogen substituent at the mentioned position appears to induce a higher stability for a free radical intermediate at the C-3 position of the benzopyran ring. A free radical enolate is most likely to be involved in the antioxidant activity of this dithiocarbamic flavanone. It is a stable intermediate that supports the influence of dithiocarbamic moiety on the antioxidant properties of the reported flavanones. [ABSTRACT FROM AUTHOR]

This paper demonstrates the potential of waste turbine oil (WTBO) as a base oil to substitute for mineral oil, which is usually used in grease formulations. This study will analyze the characteristics of used turbine oil, including its chemical composition and physical characteristics, including kinematic viscosity, viscosity index, moisture content, contamination, and density. The presence of antioxidants (butylated hydroxytoluene (BHT) and amine or phenyl-a-naphthylamine (PANA), anti-wear, and corrosion-inhibiting additives that can improve the formulated lubricating grease performance and lengthen service life are just a few of the useful remaining WTBO characteristics that can be used wisely as the base oil. It is crucial to create more environmentally friendly, economically sensible, and thrifty grease formulations to adhere to Malaysia's Green Technology Master Plan, which has outlined the strategic plans for developing green technologies. The new inventions must establish a resource- and carbon- efficient economy. The abundance of WTBO in the aviation industry and the unique characteristics of WTBO itself promise a reliable supply of base oil for lubricating grease in the future. [ABSTRACT FROM AUTHOR]

BACKGROUND RESULTS CONCLUSION The unintended co‐extraction of chlorophylls during the recovery of polyphenols from plant sources yields green‐coloured phenolic extracts with limited use in colour‐sensitive foods. This study aimed at decolourizing the ethanolic extracts of sugar beet leaves using a UV‐A treatment (390 nm).Exposure of the phenolic extracts to 30 UV‐A LEDs at 8.64 J m−2 radiation dose decreased the total chlorophyll content by 69.23% and reduced the greenness parameter (−a*) significantly (P < 0.05) from 27.33 ± 0.32 to 8.64 ± 0.16. Additionally, UV‐A treatment increased the content of most individual phenolic compounds (e.g. gallic acid, ferulic acid, etc.) significantly, resulting in an increase in the overall phenolic content in the extracts from 900.56 ± 14.11 μg g−1 fresh weight (FW) to a maximum of 975.09 ± 9.62 μg g−1 FW at 0.67 J m−2. However, rutin content had a significant decrease at the highest radiation dose (8.64 J m−2). The soluble sugar content (i.e. glucose and fructose) increased simultaneously with phenolic compounds after the UV‐A treatment. Although the UV treatment reduced the 2,2‐diphenyl‐1‐picrylhydrazyl radical scavenging activity, it had no significant effect on the ferrous chelating activity and the extract's ability to delay lipid oxidation in corn oil. The antioxidant activity index of the treated extract was comparable to that of butylated hydroxytoluene, a synthetic antioxidant.Key findings of this study include successful decolourization of the extract, decomposition of bound polyphenols to their free form, and maintaining the antioxidant activity of the extract in the oil system after UV‐A exposure. © 2024 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The rhizome of Boesenbergia rotunda (fingerroot) is an edible herb that has antioxidant properties and has been shown to have antimicrobial activity against Gram-positive pathogens including coagulase-positive staphylococci. However, there is no data on its activity against the primary cause of canine superficial pyoderma Staphylococcus pseudintermedius. The total phenolic and total flavonoid contents of an ethanolic B. rotunda rhizome extract were determined by the Folin-Ciocalteu method and aluminum chloride assay and antioxidant activity was determined in the 2,2-diphenyl-l-1-picrylhydrazil (DPPH) radical scavenging test. The minimum inhibitory (MIC) and minimum bactericidal (MBC) concentrations of the extract against 12 clinical isolates of S. pseudintermedius causing pyoderma were determined in broth microdilution assays and the time-kill kinetics were examined against S. pseudintermedius ATCC 49051. The extract contained high levels of flavonoids (394.01 ± 4.79 mg quercetin equivalent/g extract) and phenolic compounds (85.11 ± 2.68 mg gallic acid equivalent/g extract) and the antioxidant index of the extract was more than 95% at concentrations above 1.25 mg/mL, which was consistent with the activity of the positive control; butylated hydroxytoluene. The MIC50/90 (2/8 µg/mL) and MBC50/90 (8/32 µg/mL) values for the extract were slightly higher than cephalexin (MIC50/90 1/2 µg/mL and MBC50/90 1/4 µg/mL). In the time-kill assay, the fingerroot extract eliminated 99.9% of viable S. pseudintermedius ATCC 49051 within eight hours at 256 × MIC (512 µg/mL). The promising antioxidant effects of the extract combined with its antibacterial activity against pathogenic S. pseudintermedius isolates suggest that fingerroot rhizome extract may be an effective novel or supplementary treatment for superficial pyoderma in dogs. [ABSTRACT FROM AUTHOR]

The effects of the antioxidant, butylated hydroxytoluene (BHT), and the metal chelator, ethylenediaminetetraacetic acid (EDTA) in combination with cedarwood oil (CWO) were investigated for wood preservation against subterranean termites as well as two species of white-rot decay fungi and two species of brown-rot decay fungi. Vacuum pressure impregnation was used to treat wood blocks. Resistance of the treated wood test blocks was evaluated using a no-choice bioassay for termites and a soil bottle assay wood decay fungi. Eight treatments were tested: H2O only; BHT only; EDTA only; BHT with EDTA; CWO only; CWO with EDTA; CWO with BHT; and CWO with BHT plus EDTA. For termites, the lowest percentage wood mass losses were for the EDTA, BHT, CWO, and CWO/EDTA treatments, all of which were statistically equivalent. Correspondingly, these treatments all had the highest termite mortalities at 100%. The four species of decay fungi were affected differently by the wood treatments; however, overall CWO and EDTA gave the best protection against wood mass loss. The addition of BHT did not decrease mass loss. [ABSTRACT FROM AUTHOR]

Background The topical veterinary drug product containing fipronil and permethrin provides an effective repellent protection and high insecticidal efficacy for dogs. Objective The objective of this study was to develop a stability-indicating high-performance liquid chromatography (HPLC) method for simultaneous detection and quantification of fipronil, permethrin, their key degradation products, and butylated hydroxytoluene (BHT) in a topical drug product. Method The two active ingredients, their degradation products, and the antioxidant (BHT) were separated by a gradient elution on a Phenomenex Kinetex C18 column (150 × 3 mm, 2.6 µm particle size) maintained at 37°C with H2O “acetonitrile “isopropyl alcohol “85% H3PO4 (65.5 + 32.5 + 4/0.0053, v/v/v/v) as mobile phase A and acetonitrile (100%) as mobile phase B. The flow rate was 0.9 mL/min, and analytes were detected and quantified at 235 nm. Results The specificity of the method was demonstrated by adequate separation of fipronil, permethrin, their degradation products, and BHT in the forced degraded finished product. The linearity of the method was demonstrated in the range of 0.2% to 150% of target analytical concentration of both active ingredients and 50% to 150% for BHT. Excellent recoveries of fipronil, permethrin, and BHT in placebo spiked active ingredient solutions in the linearity range showed sufficient accuracy of the method. The LOQ and LOD of the method were determined to be 0.2% and 0.07% of the analytical concentration. A robustness study did not identify any critical parameter that adversely affected the separation and quantification. Conclusions Here, we report the development and validation of a robust, stability-indicating HPLC method for identification and assay of fipronil, permethrin, and BHT, including estimation of fipronil's and permethrin's degradation products in a topical drug product for dogs. Highlights The new HPLC method permits the acquisition of data for all analytes of interest for a topical finished drug product containing fipronil, permethrin, and BHT. [ABSTRACT FROM AUTHOR]

At present, there are few reports about the proteomics changes provoked by butylated hydroxytoluene (BHT) supplementation on cryopreserved semen in mammals. Thus, we aimed to evaluate the effects of different concentrations of BHT on goat sperm and to investigate the proteomics changes of adding BHT to cryopreserved goat (Capra hircus) sperm. Firstly, semen samples were collected from four goats, and frozen in the basic extenders containing different concentrations of BHT (0.5 mM, 1.0 mM, 2.0 mM) and a control without BHT, respectively. After thawing, the protective effects of dose-dependent replenished BHT to the freezing medium on post-thaw sperm motility, integrities of plasma membrane and acrosome, reactive oxygen species levels were confirmed, with 0.5 mM BHT being the best (B group) as compared to the control (without BHT, C group). Afterwards, TMT-based quantitative proteomic technique was performed to profile proteome of the goat sperm between C group and B group. Parallel reaction monitoring was used to confirm reliability of the data. Overall, 2,476 proteins were identified and quantified via this approach. Comparing the C and B groups directly (C vs. B), there were 17 differentially abundant proteins (DAPs) po-tentially associated with sperm characteristics and functions were identified, wherein three were upregulated and 14 were downregulated, respectively. GO annotation analysis demonstrated the potential involvement of the identified DAPs in metabolic process, multi-organism process, reproduction, reproductive process, and cellular process. KEGG enrichment analysis further indicated their potential roles in renin-angiotensin system and glutathione metabolism pathways. Together, this novel study clearly shows that BHT can effectively improve quality parameters and fertility potential of post-thawed goat sperm at the optimal concentration, and its cryoprotection may be realized through regulation of sperm metabolism and antioxidative capability from the perspective of sperm proteomic modification. [ABSTRACT FROM AUTHOR]

Coumarins represent a diverse class of natural compounds whose importance in pharmaceutical and agri-food sectors has motivated multiple novel synthetic derivatives with broad applicability. The phenolic moiety in 4-hydroxycoumarins underscores their potential to modulate the equilibrium between free radicals and antioxidant species within biological systems. The aim of this work was to assess the antioxidant activity of 18 4-hydroxycoumarin coumarin derivatives, six of which are commercially available and the other 12 were synthesized and chemically characterized and described herein. The 4-hydroxycoumarins were prepared by a two steps synthetic strategy with satisfactory yields. Their antioxidant potential was evaluated through three in vitro methods, two free radical-scavenging assays (DPPH• and ABTS•+) and a metal chelating activity assay. Six synthetic coumarins (4a, 4g, 4h, 4i, 4k, 4l) had a scavenging capacity of DPPH• higher than butylated hydroxytoluene (BHT) (IC50 = 0.58 mmol/L) and compound 4a (4-hydroxy-6-methoxy-2 H-chromen-2-one) with an IC50 = 0.05 mmol/L outperformed both BHT and ascorbic acid (IC50 = 0.06 mmol/L). Nine hydroxycoumarins had a scavenging capacity against ABTS•+ greater (C3, 4a, 4c) or comparable (C1, C2, C4, C6, 4g, 4l) to Trolox (IC50 = 34.34 µmol/L). Meanwhile, the set had a modest ferrous chelation capacity, but most of them (C2, C5, C6, 4a, 4b, 4h, 4i, 4j, 4k, 4l) reached up to more than 20% chelating ability percentage. Collectively, this research work provides valuable structural insights that may determine the scavenging and metal chelating activity of 4-hydroxycoumarins. Notably, substitutions at the C6 position appeared to enhance scavenging potential, while the introduction of electron-withdrawing groups showed promise in augmenting chelation efficiency. [ABSTRACT FROM AUTHOR]

Objective: Naphthoquinones are compounds that are common in nature which have biological activity depending on the attachment of new substituted groups. Methods: In the present study, the inhibition effects of newly synthesized naphthoquinones derivatives (I), (IIa–IId) on alpha-glucosidase, alpha-amylase, xanthine oxidase, and trypsin and antioxidant properties were investigated by in vitro studies. Results and Discussion: The synthesized compounds (IIb) (784.04 ± 79.95 µM) and (IId) (477.55 ± 1.18µM) compounds on alpha-glucosidase activity were found to have a lower IC50 value when compared to the positive control—acarbose (1628.48 ± 32.89 µM) as well as molecular docking results. Compound (IId) (690.45 ± 5.48 µM) had high 2,2-diphenyl-1-picrylhydrazil (DPPH) scavenging activity, with an IC50 value lower than that of the positive control butylated hydroxytoluene (BHT, 961.92 ± 6.41 µM). The results indicated that synthesized compounds had a dose-dependent inhibition effect on the enzymes. Conclusions: In the in vitro studies, compounds (IIb) and (IId) were strong alpha-glucosidase inhibitors as well as compound (IId) had high DPPH scavenging activity. [ABSTRACT FROM AUTHOR]

Pulu Mandoti, a local red rice (Oryza sativa L.) variety popular among Sulawesi residents, has gained recognition for its perceived health benefits, especially as a preferred dietary option for individuals with diabetes or those seeking to prevent obesity. Given the increasing consumption of mushrooms, particularly Pleurotus species, renowned for their nutritional and medicinal attributes, this study delves into the transformative effects of Pleurotus spp. fermentation on Pulu Mandoti, the indigenous rice variety. Proximate analysis disclosed elevated dry matter (91.99 ± 0.61%), crude protein (8.55 ± 0.15%), and crude fat (1.34 ± 0.05%) in Pleurotus cystidiosus fermentation compared to Pleurotus ostreatus and Pleurotus djamor. Concurrently, antioxidant and antidiabetic activities were notably improved in all Pleurotus fermentations. Pulu Mandoti fermented with P. cystidiosus outperformed other treatments, aligning with molecular docking results pinpointing 11-Eicosenoic acid, methyl ester, and butylated hydroxytoluene as optimal interactors with antioxidant receptors 5O0x and 2CKJ. Butylated hydroxytoluene demonstrated interactions with the antidiabetic receptor 2QV4, along with 9-Octadecenoic acid, methyl ester. These compounds, previously unreported in Pleurotus, displayed promising attributes as antioxidants and antidiabetic agents. Furthermore, the investigation delved into the fatty acid profiles, emphasizing the diverse range of potential bioactive compounds in fermented Pulu Mandoti. The findings of this research present a potential functional food rich in natural antioxidants and antidiabetic compounds, highlighting the yet undiscovered capabilities of Pleurotus spp. fermentation in augmenting the nutritional composition and bioactivity of indigenous rice varieties, specifically Pulu Mandoti. [ABSTRACT FROM AUTHOR]

The aim of this study was to assess the effect of replacing powdered egg yolk (PEY) with soybean lecithin (SL) or butylated hydroxytoluene (BHT) on ram sperm cryopreservation. Two ejaculates/male were collected via artificial vagina from 8 rams during breeding season. Ejaculates were pooled and washed twice by centrifugation. The pellet was divided into three aliquots, diluted in a Tris-based media with 5% glycerol containing PEY (15%), SL (1%) or BHT (0.6 mM) and cooled for 4 h at 5 °C before freezing. Sperm motility, plasma and acrosome membrane integrity and mitochondria activity as lipid peroxidation were assessed immediately after thawing and after 4 h of resilience incubation in a modified PBS at 38 ºC. After thawing, sperm extended in BHT showed the poorest quality compared to sperm extended in PEY and SL. Similar total and progressive motility were observed in sperm preserved in PEY and SL media. Plasma membrane integrity, however, was significantly higher in sperm extended in SL, although most of them with non-functional mitochondria. Acrosome damage was significant lower in SL sperm samples compared to PEY samples. Highest level of lipid peroxidation was found in sperm preserved in PEY. Resilience test had a negative effect (P < 0.05) on plasma and acrosome membrane integrity in all samples, and on progressive motility only in sperm preserved in PEY. In conclusion, soy lecithin could be a potential alternative to PEY for ram cryopreservation, although its adverse effect on sperm mitochondria function has to be strongly considered. [ABSTRACT FROM AUTHOR]

Mayonnaise is an emulsion of oil that is susceptible to lipid oxidation, which can cause spoilage and the formation of harmful compounds. This study aims to evaluate the impact of Syrian apple and grape vinegar on the oxidative stability of mayonnaise and compare the use of natural antioxidants to synthetic ones such as butylated hydroxyanisole and butylated hydroxytoluene. The study measured total phenol content, radical scavenging activity, and identified some phenolic compounds by High Performance Liquid Chromatography (HPLC). The rancidity of mayonnaise was examined using peroxide value and thiobarbituric acid number. The fatty acids content in the mayonnaise samples was examined using gas chromatography. Vinegar samples containing high concentrations of phenolic antioxidants showed high ability to scavenge free radicals. The antioxidants in vinegar protected the mayonnaise samples from primary and secondary oxidation, and there was no statistically significant difference between the ratio of unsaturated fatty acids in the samples containing vinegar at the beginning and at the end of the storage period. The study shows the significance of using vinegar to protect mayonnaise from deterioration and increase its shelf life, in addition to its role as a dressing. [ABSTRACT FROM AUTHOR]

In this study, chitosan (CS)‐based macromolecular synergistic antioxidant CS‐UC‐TDAA containing carbon–carbon double bond, hindered phenol, and aromatic secondary amine was prepared by using CS,10‐undecenoyl chloride (UC), p‐aminobenzoic acid (AA), 3,5‐di‐tert‐butyl‐2‐hydroxybenzaldehyde (TD) via esterification, Schiff base reaction, and acylation reaction. The molecular structure of CS‐UC‐TDAA was characterized by fourier transform infrared, 1H NMR, and elemental analysis. The effects of macromolecular synergistic antioxidant CS‐UC‐TDAA, small molecular antioxidant TDAA, commercial antioxidant 4020, and butylated hydroxytoluene (BHT) on the antiaging properties of styrene‐butadiene rubber (SBR)/SiO2 composites were systematically studied by accelerated thermo‐oxidative aging test, thermogravimetric (TG) analysis, and methanol Soxhlet extraction experiment. The experimental results show that CS‐UC‐TDAA has more outstanding thermo‐oxidative aging resistance, thermal stability, and solvent extraction resistance in SBR/SiO2 composites than TDAA, 4020, and BHT. The macromolecular synergistic antioxidant CS‐UC‐TDAA endows SBR/SiO2 composites with more durable antiaging performance, which is attributed to the synergistic antioxidant effect of its hindered phenol and aromatic secondary amine groups. Additionally, its resistance to migration and extraction is facilitated by the crosslinking of the carbon–carbon double bond with the rubber and its substantial molecular weight. [ABSTRACT FROM AUTHOR]

Copyright of China Condiment is the property of China Condiment and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objectives: The current research delves into ethnopharmacology, specifically focusing on the influence of extraction solvent on parameters such as total phenol, flavonoid, coumarin, tannin, antioxidant, and antibacterial activity. This investigation spans different parts of Melilotus officinalis L. within two northern provinces of Iran. It is a holistic approach, exploring both traditional applications and the scientific impact of extraction methods on the plant's bioactive properties. Materials and Methods: In various observational studies across different fields, ethnopharmacological data were acquired by collecting various parts of the M. officinalis plant (flowers, stem, and root) at different blooming stages from Charbagh Mountain (2340 msl) and Gorgan region (200 msl) between June and July 2016. "Ethanol and water extracts" of these plant parts were obtained through the maceration method. The quantification of "total phenol" (TP), "flavonoid" (TF), coumarin (CO), and tannin (TA) content in the extracts was conducted using spectrophotometry. The in vitro "antioxidant capacity" of the extracts was assessed through the DPPH free radical scavenging assay, the total antioxidant capacity (TAC) assay, and the reducing power assay. Additionally, the antibacterial activity of the extracts was examined against nine gram-positive and gram-negative bacteria using disc diffusion and minimum inhibitory concentration (MIC) assays. Results: Based on the results, the ethanol extract obtained from the flowers of M. officinalis collected at a high altitude (2340 meters) in Semnan province demonstrated the highest concentrations of total phenols (38.08 ± 0.13 mgGA/g), flavonoids (62.04 ± 0.01 mgQU/g), coumarins (19.32 ± 0.08 mL/g), and tannins (33.89 mg/g). Notably, coumarin and tannins were absent in the water extract. The ethanol extract from the flowers in Semnan province exhibited superior antioxidant activity, particularly in the DPPH method (IC50=10.61 ± 0.81 µg/mL), surpassing the efficacy of butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT). Moreover, the ethanol extract from high-altitude flowers displayed potent antimicrobial activity against gram-positive bacteria, specifically Staphylococcus aureus, S. epidermidis, and Bacillus cereus, with inhibition zone (IZ) values of 19.1 ± 0.01 mm, 15.8 ± 0.2 mm, and 14.4 ± 1.12 mm, respectively. The MIC values against these bacteria were 24.5 µg/mL, respectively. In summary, the ethanol extract exhibited greater effectiveness against gram-positive bacteria compared to gram-negative bacteria. Conclusions: Our results indicate a robust association among the extraction solvent, plant part, phytochemical composition, antibacterial efficacy, and antioxidant potential of M. officinalis L. Specifically, the ethanol extract derived from the flowers displayed the most significant antioxidant and antibacterial characteristics. This implies that the ethanol extract from the flowers of M. officinalis L. holds promise as a valuable reservoir of natural antioxidants, aligning with its traditional utilization in addressing various infections. [ABSTRACT FROM AUTHOR]

This study aims to thoroughly examine the chemicals and effects of the ethanol extract from T. polium's upper parts. We used the Soxhlet method for extraction, resulting in an extract with a significant yield of 20.6%. Qualitative analysis identified a variety of compounds, such as tannins, saponins, reducing compounds, terpenoids, quinones, and alkaloids. In quantitative analysis using the colorimetric method, we found the extract was rich in total flavonoids (20.78 mg equivalent QE/g DW extract) and total polyphenols (227.43 mg equivalent GAE/g DW extract). To assess antioxidant potential, we used the ferric reducing antioxidant power (FRAP) method, with ascorbic acid and butylated hydroxytoluene (BHT) as standards. The extract showed moderate activity in both the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and FRAP methods at concentrations of 65 µg/mL and 21 mg/mL, respectively. Additionally, we tested the ethanolic extract against various bacteria using the disk diffusion technique on agar medium. The results indicated that the T. polium extract had moderate effectiveness against Gram-negative bacteria like Pseudomonas aeruginosa ATCC 9027 and Escherichia coli ATCC 8739, as well as Gram-positive bacteria like Staphylococcus aureus ATCC 6538 and Bacillus subtilis ATCC 6633. We further investigated the composition of the ethanolic extract through LC-MS/MS analysis, establishing a detailed profile of phenolic compounds, with six flavonoids identified as the main polyphenolic constituents. This thorough evaluation provides insights into the potential therapeutic uses of T. polium. [ABSTRACT FROM AUTHOR]

This study evaluated the feasibility and optimization of the spouted bed drying to produce standardized dried Camellia sinensis extract. We explored processing variables such as inert particle type (Teflon® or glass beads), drying aid concentration (colloidal SiO2—Aerosil 200®), and drying inlet temperatures (80, 110, or 140 °C). Regression analysis of the results allowed for constructing response surface graphs, highlighting variable impacts on product properties and spouted bed performance. The optimal conditions involved Teflon® beads and a drying temperature of 110 °C, resulting in a product with excellent moisture content, water activity, and active constituent retention. The product demonstrated significant antioxidant activity in DPPH assays, with an IC50 between 4.4 to 6.7 µg/mL, surpassing the synthetic antioxidant butylated hydroxytoluene (BHT), which had an IC50 of 12.5 µg/mL. Rancimat assays showed that the dried extract effectively protected crude sunflower oil from oxidation, increasing its shelf life from 8.2 to 13.1 months. [ABSTRACT FROM AUTHOR]

Synthetic dyes are persistent organic environmental pollutants that can cause extensive damage to living beings and to the ecosystem as a whole. Cost-effective, sustainable, and efficient strategies to deal with this type of pollution are necessary as it commonly resists conventional water treatment methods. Silver nanoparticles (AgNPs) synthesized using the aqueous extract from the leaves, stem, and fruits of Leucaena leucocephala (Leucena) were produced and characterized through UV–vis, TEM, EDS, SDL, XPS, XRD, and zeta potential, and they proved to be able to promote adsorption to remediate methylene blue and tartrazine pollution in water. The nanoremediation was performed and did not require direct exposure to sunlight or any special lamp or a specific reduction agent. The AgNPs produced using the extract from the leaves exhibited the best performance in nanoremediation and also presented antioxidant activity that surpassed the one from butylated hydroxytoluene (BHT). Consequently, it is an interesting nanotool to use in dye nanoremediation and/or as an antioxidant nanostructure. [ABSTRACT FROM AUTHOR]

This study is the beginning of the research that focuses on unconventional ionic liquids (ILs) hydrodistillation (HD) extraction of the essential oil of Heliotropium europaeum L. using ILs as additives. Two ILs based on 1- butyl-3-methylimidazolium were used by switching the anions (Cl- and PF6 -). The effect of mass percent of the added ILs on its yield and composition was evaluated. Compared to the conventional HD, ILs-HD gives a higher yield of essential oils (0.10-0.36 %). Particularly, with [C4mim][PF6], the observation of morphological changes using scanning electron microscopy (SEM) confirmed the effectiveness of the ionic liquid in this distillation process. The GC- -MS analysis of essential oils (EOs) revealed the presence of sixty-six compounds in HD, ILs-HD methods. Gas chromatography-mass spectrometry analysis of the EOs revealed the predominance of eugenol (1.70-72.35 %), butylated hydroxytoluene (8.95-65.39 %) and phytol (18.20 %). The new distillation methods of H. europaeum with ILs identifies more compounds (50 compounds in ILs-HD [C4mim][PF6]; 22 compounds in ILs-HD ([C4mim][Cl]) than conventional hydrodistillation (25 compounds in HD). Therefore, the ILs- -based hydrodistillation approach is superior in improving the production of EOs. It is important to emphasize that the data presented in this study are not yet available for any of this Algerian Heliotropium species of genus and present the great potential of this medicinal plant as a source of novel bioactive extracts with possible therapeutic uses. [ABSTRACT FROM AUTHOR]

This research explores the eco-friendly synthesis of silver nanoparticles (AgNPs) using Cassia occidentalis L. seed extract. Various analytical techniques, including UV–visible spectroscopy, transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscopy (EDX), were employed for comprehensive characterization. The UV–visible spectra revealed a distinct peak at 425 nm, while the seed extract exhibited peaks at 220 and 248 nm, indicating the presence of polyphenols and phytochemicals. High-resolution TEM unveiled spherical and oval-shaped AgNPs with diameters ranging from 6.44 to 28.50 nm. The SEM exhibiting a spherical shape and a polydisperse nature, thus providing insights into the morphology of the AgNPs. EDX analysis confirmed the presence of silver atoms at 10.01% in the sample. XRD results unequivocally confirm the crystalline nature of the AgNPs suspension, thereby providing valuable insights into their structural characteristics and purity. The antioxidant properties of AgNPs, C. occidentalis seed extract, and butylated hydroxytoluene (BHT) were assessed, revealing IC50 values of 345, 500, and 434 μg/mL, respectively. Antibacterial evaluation against Bacillus subtilis, Staphylococcus aureus, and Escherichia coli demonstrated heightened sensitivity of bacteria to AgNPs compared to AgNO3. Standard antibiotics, tetracycline, and ciprofloxacin, acting as positive controls, exhibited substantial antibacterial efficacy. The green-synthesized AgNPs displayed potent antibacterial activity, suggesting their potential as a viable alternative to conventional antibiotics for combating pathogenic bacterial infections. Furthermore, potential biomedical applications of AgNPs were thoroughly discussed. [ABSTRACT FROM AUTHOR]

The aim of this work was to give as much information as possible on Rosa canina dried fruit that is commercially available in Serbia. In order to provide the chemical composition, the UHPLC-DAD-ESI-MS method was employed for both polar and non-polar extracts of samples obtained with a solvent mixture consisting of hexane, acetone, and ethanol in a volume ratio of 2:1:1, respectively, and 0.05% (w/v) butylated hydroxytoluene. In addition, the total content levels of lycopene, β-carotene, total polyphenols, and flavonoids were determined by means of UV-vis spectrophotometry. The antioxidant activity was tested by applying four different methods: ABTS, DPPH, FRAP, and CUPRAC. Overall, nine compounds were identified. The results of chemical composition analysis were used as the basis for the interpretation of the calculated results for the antioxidant and antimicrobial activity. The obtained results for R. canina dried fruit extract are as follows: β-carotene—7.25 [mg/100 g fruit weight]; lycopene—2.34 (mg/100 g FW); total polyphenol content (TPC)—2980 [mg GAE/kg FW]; total flavonoid content (TFC)—1454 [mg CE/kg FW]; antioxidant activity—ABTS 12.3 [μmol/100 g FW], DPPH 6.84, FRAP 52.04, and CUPRAC 15,425; and antimicrobial activity—Staphylococcus aureus MIC/MMC 4/0 [mg∙mL−1], Enterococcus faecalis 4/0, Bacillus cereus 4/0, Escherichia coli 4/0, Salmonella enteritidis 4/4, Enteroabacter aerogenes 4/0, Pseudomonas aeruginosa 2/0, and Candida albicans 2/0. [ABSTRACT FROM AUTHOR]

Both rosemary (Rosmarinus officinalis) and marjoram (Origanum majorana) are abundant in phenolic compounds, exhibiting exceptional antioxidant activity. This study aims to assess the impact of rosemary and marjoram extracts on the stability of sunflower oil during storage and repeated heating. Sunflower oil supplemented with herbal extracts or butylated hydroxytoluene (BHA) at a concentration of 200 ppm was stored for six months under light and dark conditions at room temperature. Peroxide value (PV), p-anisidine value (An-V), and total oxidation (TOTOX) value were measured to monitor lipid oxidation progression. A significant difference (P < 0.05) was observed between light and dark storage for all studied samples regarding oxidation parameters. The ethanolic extract of rosemary exhibited higher antioxidant activity compared to BHA and other extracts. Furthermore, sunflower oil supplemented with the ethanolic extract of rosemary underwent weekly treatment at 100 ℃ for 30 min over four consecutive weeks. Although all oxidation indicators increased during repeated heating, the addition of rosemary and marjoram extracts as well as BHA significantly reduced these indicators. These findings demonstrate that both rosemary extracts and marjoram extracts can serve as natural antioxidants in edible oils. [ABSTRACT FROM AUTHOR]

The present study aimed to evaluate the antioxidant capacity of ethanolic extracts from post-harvest sunflower and maize stalk residues, and their impact on the chemical composition, physicochemical parameters, lipid oxidative stability, microbiological properties, and sensory characteristics of pork liver pâtés over a 90-day storage period. Four formulations were prepared: a control group (CON), a batch with butylated hydroxytoluene as a synthetic antioxidant (BHT), 1% ethanolic extract from sunflower residues (SSRE), and 1% ethanolic extract from maize residues (MSRE). The MSRE had a higher total phenol content and showed better antioxidant activity relative to the SSRE (p < 0.01). The addition of SSRE decreased the lightness and increased the redness in the pork liver pâtés, with these pâtés showing the highest total color difference compared to the control (p < 0.01). The crop extracts increased the n-6 and total PUFA contents in pâtés and improved the PUFA/SFA ratio (p < 0.01). Formulations containing crop residue extracts showed higher TBARs and POV values than the control and BHT group (p < 0.01), indicating a pro-oxidant effect and accelerated lipid oxidation in pâtés during storage. As far as microbiological quality, the presence of crop residue extracts decreased the total viable count, lactic acid bacteria, and psychotropic aerobic bacteria (p < 0.01). The incorporation of crop extracts in the pork pâtés impaired their sensory quality, particularly color, odor, aroma, and flavor, and decreased their overall acceptability. These results indicated that, while the crop residue extracts were not as effective as synthetic antioxidants in preserving the lipid stability of pâtés, they demonstrated potential for enhancing the microbial quality of this type of meat product. [ABSTRACT FROM AUTHOR]

The increasing production of edible insects on an industrial scale makes it crucial to implement appropriate technologies after harvesting to process safe and high quality insect products. The aim of this work was to compare the impact of different drying treatments used in the production of flour from Tenebrio molitor larvae. The larvae were subjected to freeze-drying (FD), conventional drying (CD), microwave drying (MWD), microwave drying without freezing prior blanching (MWDL), and microwave drying with addition of 0.1% butylated hydroxytoluene (BHT) during the blanching of the larvae (MWDA). The studied parameters included water activity (aw), instrumental colour, chemical composition, lipid oxidative processes, antioxidant activity, as well as microbiological status. The freeze-drying and conventional drying of the larvae reduced the aw of the derived flours (p < 0.0001); however, their nutritional profile revealed lower protein (p < 0.0001) and considerably higher fat content (p < 0.0001) compared to the flours after microwave treatments. The conventional drying and microwave treatment with BHT induced significantly darker colour (p < 0.0001) in comparison to the other methods. Despite the advantages of the microwave drying as a fast and energy efficient method, it displayed some negative effects associated with low lipid stability such as higher acid value (AV) and secondary products of lipid oxidation (TBARS) (p < 0.0001). This was also observed in the MWDA flour, indicating a certain pro-oxidative effect of the BHT. Regardless of the drying method, all the flours had a low microbial load. [ABSTRACT FROM AUTHOR]

At present, there are few reports about the proteomics changes provoked by butylated hydroxytoluene (BHT) supplementation on cryopreserved semen in mammals. Thus, we aimed to evaluate the effects of different concentrations of BHT on goat sperm and to investigate the proteomics changes of adding BHT to cryopreserved goat (Capra hircus) sperm. Firstly, semen samples were collected from four goats, and frozen in the basic extenders containing different concentrations of BHT (0.5 mM, 1.0 mM, 2.0 mM) and a control without BHT, respectively. After thawing, the protective effects of dose-dependent replenished BHT to the freezing medium on post-thaw sperm motility, integrities of plasma membrane and acrosome, reactive oxygen species levels were confirmed, with 0.5 mM BHT being the best (B group) as compared to the control (without BHT, C group). Afterwards, TMT-based quantitative proteomic technique was performed to profile proteome of the goat sperm between C group and B group. Parallel reaction monitoring was used to confirm reliability of the data. Overall, 2,476 proteins were identified and quantified via this approach. Comparing the C and B groups directly (C vs. B), there were 17 differentially abundant proteins (DAPs) po-tentially associated with sperm characteristics and functions were identified, wherein three were upregulated and 14 were downregulated, respectively. GO annotation analysis demonstrated the potential involvement of the identified DAPs in metabolic process, multi-organism process, reproduction, reproductive process, and cellular process. KEGG enrichment analysis further indicated their potential roles in renin-angiotensin system and glutathione metabolism pathways. Together, this novel study clearly shows that BHT can effectively improve quality parameters and fertility potential of post-thawed goat sperm at the optimal concentration, and its cryoprotection may be realized through regulation of sperm metabolism and antioxidative capability from the perspective of sperm proteomic modification.

Butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA) are two antioxidants that have been extensively used in many applications. Both are well known for their debatable health risks due to their multiple intake sources. Therefore, conservative limits are set for them in different regulations adapted to the matrices in which they exist. Here we present a simple spectrofluorimetric method for the determination of BHT and BHA based on their native fluorescence and synchronous scanning mode. The type of solvent and the interval between emission and excitation wavelengths were carefully optimized. Under the optimized conditions, good linearities were obtained between the emission intensity and the corresponding concentrations of BHT and BHA over the range of 3–18 µg/mL and 0.1–7 µg/mL, respectively with a good correlation coefficient (r > 0.99). The limits of detection were 0.9 and 0.02 µg/mL, and the quantification limits were 3 and 0.05 µg/mL for BHT and BHA, respectively. The suggested procedure was validated according to ICH guidelines Q2 (R1). Furthermore, the method's greenness was assessed by three different methods, and it proved to be eco-reasonable. The method was successfully applied to the determination of BHT and BHA in pharmaceutical formulations. We also applied the suggested method for monitoring the residual BHA in conventional, powdered milk and butter, with good recovery in spiked samples. [ABSTRACT FROM AUTHOR]