Search

Your search keyword '"Butterly, S"' showing total 23 results
Start Over Author "Butterly, S"
23 results on '"Butterly, S"'

1. Elevation of cell-associated HIV-1 transcripts in CSF CD4+ T cells, despite effective antiretroviral therapy, is linked to brain injury

Author

Suzuki, K, Zaunders, J ; https://orcid.org/0000-0002-5912-5989, Gates, TM ; https://orcid.org/0000-0003-0113-8282, Levert, A ; https://orcid.org/0000-0003-4352-0228, Butterly, S, Liu, Z, Ishida, T, Palmer, S, Rae, CD ; https://orcid.org/0000-0003-0673-8084, Jugé, L ; https://orcid.org/0000-0002-4136-8069, Cysique, LA ; https://orcid.org/0000-0002-7711-6373, Brew, BJ ; https://orcid.org/0000-0002-9885-8261, Suzuki, K, Zaunders, J ; https://orcid.org/0000-0002-5912-5989, Gates, TM ; https://orcid.org/0000-0003-0113-8282, Levert, A ; https://orcid.org/0000-0003-4352-0228, Butterly, S, Liu, Z, Ishida, T, Palmer, S, Rae, CD ; https://orcid.org/0000-0003-0673-8084, Jugé, L ; https://orcid.org/0000-0002-4136-8069, Cysique, LA ; https://orcid.org/0000-0002-7711-6373, and Brew, BJ ; https://orcid.org/0000-0002-9885-8261

Published
2022

6. An Experimental Series Investigating the Effects of Euglycaemic Hyperinsulinaemia on Myocardial Blood Flow Reserve in Healthy Individuals and Perfusion Defect Size in Patients Presenting With Acute Myocardial Infarction

Author

Nam, M., primary, Meneses, A., additional, Anstey, C., additional, Askew, C., additional, Hickman, I., additional, Bailey, T., additional, Quah, J., additional, Senior, R., additional, Cox, S., additional, Poulter, R., additional, Butterly, S., additional, Fryer, M., additional, Russell, A., additional, Stanton, T., additional, and Greaves, K., additional

Published
2018
Full Text
View/download PDF

22. Elevation of cell-associated HIV-1 transcripts in CSF CD4+ T cells, despite effective antiretroviral therapy, is linked to brain injury.

Author

Suzuki K, Zaunders J, Gates TM, Levert A, Butterly S, Liu Z, Ishida T, Palmer S, Rae CD, Jugé L, Cysique LA, and Brew BJ

Subjects
Humans, CD4-Positive T-Lymphocytes, Leukocytes, Mononuclear, HIV-1 genetics, HIV Seropositivity, HIV Infections drug therapy, Brain Injuries
Abstract

Antiretroviral therapy (ART) can attain prolonged undetectable HIV-1 in plasma and cerebrospinal fluid (CSF), but brain injury remains prevalent in people living with HIV-1 infection (PLHIV). We investigated cell-associated (CA)-HIV-1 RNA transcripts in cells in CSF and blood, using the highly sensitive Double-R assay, together with proton Magnetic Resonance Spectroscopy ( 1 H MRS) of major brain metabolites, in sixteen PLHIV. 14/16 CSF cell samples had quantifiable CA-HIV-1 RNA, at levels significantly higher than in their PBMCs (median 9,266 vs 185 copies /106 CD4+ T-cells; p<0.0001). In individual PLHIV, higher levels of HIV-1 transcripts in CSF cells were associated with greater brain injury in the frontal white matter (Std β=-0.73; p=0.007) and posterior cingulate (Std β=-0.61; p=0.03). 18-colour flow cytometry revealed that the CSF cells were 91% memory T-cells, equally CD4+ and CD8+ T-cells, but fewer B cells (0.4 %), and monocytes (3.1%). CXCR3 + CD49d + integrin β7-, CCR5 + CD4 + T-cells were highly enriched in CSF, compared with PBMC (p <0.001). However, CA-HIV-1 RNA could not be detected in 10/16 preparations of highly purified monocytes from PBMC, and was extremely low in the other six. Our data show that elevated HIV-1 transcripts in CSF cells were associated with brain injury, despite suppressive ART. The cellular source is most likely memory CD4 + T cells from blood, rather than trafficking monocytes. Future research should focus on inhibitors of this transcription to reduce local production of potentially neurotoxic and inflammatory viral products.

Published
2022
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results