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49 results on '"Butkevich, Alexey N."'

3. Photoactivatable Xanthone (PaX) Dyes Enable Quantitative, Dual Color, and Live‐Cell MINFLUX Nanoscopy.

Author

Remmel, Michael, Matthias, Jessica, Lincoln, Richard, Keller‐Findeisen, Jan, Butkevich, Alexey N., Bossi, Mariano L., and Hell, Stefan W.

Subjects
NUCLEAR pore complex, FLUORESCENT dyes, MOLECULAR switches, FLUORESCENT proteins, SPECTRAL imaging
Abstract

The single‐molecule localization concept MINFLUX has triggered a reevaluation of the features of fluorophores for attaining nanometer‐scale resolution. MINFLUX nanoscopy benefits from temporally controlled fluorescence ("on"/"off") photoswitching. Combined with an irreversible switching behavior, the localization process is expected to turn highly efficient and quantitative data analysis simple. The potential in the recently reported photoactivable xanthone (PaX) dyes is recognized to extend the list of molecular switches used for MINFLUX with 561 nm excitation beyond the fluorescent protein mMaple. The MINFLUX localization success rates of PaX560, PaX+560, and mMaple are quantitatively compared by analyzing the effective labeling efficiency of endogenously tagged nuclear pore complexes. The PaX dyes prove to be superior to mMaple and on par with the best reversible molecular switches routinely used in single‐molecule localization microscopy. Moreover, the rationally designed PaX595 is introduced for complementing PaX560 in dual color 561 nm MINFLUX imaging based on spectral classification and the deterministic, irreversible, and additive‐independent nature of PaX photoactivation is showcased in fast live‐cell MINFLUX imaging. The PaX dyes meet the demands of MINFLUX for a robust readout of each label position and fill the void of reliable fluorophores dedicated to 561 nm MINFLUX imaging. [ABSTRACT FROM AUTHOR]

Published
2024
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27. Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super‐Resolution STED Microscopy in Living Cells

Author

Butkevich, Alexey N., primary, Mitronova, Gyuzel Yu., additional, Sidenstein, Sven C., additional, Klocke, Jessica L., additional, Kamin, Dirk, additional, Meineke, Dirk N. H., additional, D'Este, Elisa, additional, Kraemer, Philip‐Tobias, additional, Danzl, Johann G., additional, Belov, Vladimir N., additional, and Hell, Stefan W., additional

Published
2016
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28. Fluoreszierende Rhodamine und fluorogene Carbopyronine für die STED‐Mikroskopie lebender Zellen

Author

Butkevich, Alexey N., primary, Mitronova, Gyuzel Yu., additional, Sidenstein, Sven C., additional, Klocke, Jessica L., additional, Kamin, Dirk, additional, Meineke, Dirk N. H., additional, D'Este, Elisa, additional, Kraemer, Philip‐Tobias, additional, Danzl, Johann G., additional, Belov, Vladimir N., additional, and Hell, Stefan W., additional

Published
2016
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30. PONy Dyes: Direct Addition of P(III) Nucleophiles to Organic Fluorophores

Author

Butkevich, Alexey N., Sednev, Maksim V., Shojaei, Heydar, Belov, Vladimir N., and Hell, Stefan W.

Abstract

Nucleophilic addition of phosphinic acid, phosphites, sodium dialkyl phosphites, phosphoramidites, phosphinites, and phosphonites to highly polarized or cationic fluorophores, followed by oxidation, results in new “PONy” dyes with auxochromic phosphinate, phosphonate, or phosphonamidate groups. The reaction was applied to a wide variety of coumarins, (thio)pyronins, and N-alkylacridinium and 5,6-dihydrobenzo[c]xanthen-12-ium salts as well as a meso-chlorinated BODIPY to provide compact dyes with red-shifted absorption and emission bands and Stokes shifts up to 8200 cm–1.

Published
2024
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31. Two-Color 810 nm STED Nanoscopy of Living Cells with Endogenous SNAP-Tagged Fusion Proteins

Author

Butkevich, Alexey N., Ta, Haisen, Ratz, Michael, Stoldt, Stefan, Jakobs, Stefan, Belov, Vladimir N., and Hell, Stefan W.

Abstract

A 810 nm STED nanoscopy setup and an appropriate combination of two fluorescent dyes (Si-rhodamine 680SiRand carbopyronine 610CP) have been developed for near-IR live-cell super-resolution imaging. Vimentin endogenously tagged using the CRISPR/Cas9 approach with the SNAP tag, together with a noncovalent tubulin label, provided reliable and cell-to-cell reproducible dual-color confocal and STED imaging of the cytoskeleton in living cells.

Published
2024
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45. Two-Step One-Pot Synthesis of Benzoannulated Spiroacetals by Suzuki–Miyaura Coupling/Acid-Catalyzed Spiroacetalization.

Author

Butkevich, Alexey N., Corbu, Andrei, Meerpoel, Lieven, Stansfield, Ian, Angibaud, Patrick, Bonnet, Pascal, and Cossy, Janine

Subjects
*CHEMICAL synthesis, *SUZUKI reaction, *COUPLING reactions (Chemistry), *SPIRO compounds, *ANNULATION, *GLYCALS
Abstract

Substituted benzoannulated spiroacetals were prepared from (2-haloaryl)alkyl alcohols and dihydropyranyl or dihydrofuranyl pinacol boronates using a Suzuki–Miyaura coupling followed by an acid-catalyzed spirocyclization. Application of the reaction to a glycal boronate provides an approach to annulated spiroacetals in enantiopure form. [ABSTRACT FROM AUTHOR]

Published
2012
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46. Two-Color 810 nm STED Nanoscopy of Living Cells with Endogenous SNAP-Tagged Fusion Proteins.

Author

Butkevich AN, Ta H, Ratz M, Stoldt S, Jakobs S, Belov VN, and Hell SW

Subjects
CRISPR-Cas Systems genetics, Cell Line, Tumor, Color, Fluorescence, Fluorescent Dyes chemical synthesis, Fluorescent Dyes chemistry, Fluorescent Dyes radiation effects, Gene Editing, Humans, Infrared Rays, Microscopy, Confocal, Organosilicon Compounds chemical synthesis, Organosilicon Compounds chemistry, Organosilicon Compounds radiation effects, Quinolizines chemical synthesis, Quinolizines chemistry, Quinolizines radiation effects, Recombinant Fusion Proteins genetics, Vimentin genetics, Cytoskeleton metabolism, Fluorescent Dyes pharmacology, Microscopy, Fluorescence methods, Organosilicon Compounds pharmacology, Quinolizines pharmacology
Abstract

A 810 nm STED nanoscopy setup and an appropriate combination of two fluorescent dyes (Si-rhodamine 680SiR and carbopyronine 610CP) have been developed for near-IR live-cell super-resolution imaging. Vimentin endogenously tagged using the CRISPR/Cas9 approach with the SNAP tag, together with a noncovalent tubulin label, provided reliable and cell-to-cell reproducible dual-color confocal and STED imaging of the cytoskeleton in living cells.

Published
2018
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47. Hydroxylated Fluorescent Dyes for Live-Cell Labeling: Synthesis, Spectra and Super-Resolution STED.

Author

Butkevich AN, Belov VN, Kolmakov K, Sokolov VV, Shojaei H, Sidenstein SC, Kamin D, Matthias J, Vlijm R, Engelhardt J, and Hell SW

Abstract

Hydroxylated rhodamines, carbopyronines, silico- and germanorhodamines with absorption maxima in the range of 530-640 nm were prepared and applied in specific labeling of living cells. The direct and high-yielding entry to germa- and silaxanthones tolerates the presence of protected heteroatoms and may be considered for the syntheses of various sila- and germafluoresceins, as well as -rhodols. Application in stimulated emission depletion (STED) fluorescence microscopy revealed a resolution of 50-75 nm in one- and two-color imaging of vimentin-HaloTag fused protein and native tubulin. The established structure-property relationships allow for prediction of the spectral properties and the positions of spirolactone/zwitterion equilibria for the new analogues of rhodamines, carbo-, silico-, and germanorhodamines using simple additive schemes., (© 2017 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.)

Published
2017
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48. Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super-Resolution STED Microscopy in Living Cells.

Author

Butkevich AN, Mitronova GY, Sidenstein SC, Klocke JL, Kamin D, Meineke DN, D'Este E, Kraemer PT, Danzl JG, Belov VN, and Hell SW

Subjects
Fluorescent Dyes chemistry, HeLa Cells, Humans, Microscopy methods, Pyronine chemistry, Rhodamines chemistry
Abstract

A range of bright and photostable rhodamines and carbopyronines with absorption maxima in the range of λ=500-630 nm were prepared, and enabled the specific labeling of cytoskeletal filaments using HaloTag technology followed by staining with 1 μm solutions of the dye-ligand conjugates. The synthesis, photophysical parameters, fluorogenic behavior, and structure-property relationships of the new dyes are discussed. Light microscopy with stimulated emission depletion (STED) provided one- and two-color images of living cells with an optical resolution of 40-60 nm., (© 2016 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.)

Published
2016
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49. Synthesis of substituted indenones and indanones by a Suzuki-Miyaura coupling/acid-promoted cyclisation sequence.

Author

Butkevich AN, Ranieri B, Meerpoel L, Stansfield I, Angibaud P, Corbu A, and Cossy J

Subjects
Boronic Acids chemistry, Catalysis, Chemistry Techniques, Synthetic, Cyclization, Hydrogen-Ion Concentration, Indans chemical synthesis, Indans chemistry
Abstract

A one-pot Suzuki-Miyaura cross-coupling/acid-catalyzed cyclisation leading to indenones and indanones in modest to good yields is reported.

Published
2014
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