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4. Long-term intake of Lactobacillus helveticus enhances bioavailability of omega-3 fatty acids in the mouse retina

Author

Lapaquette, Pierre, primary, Terrat, Sébastien, additional, Proukhnitzky, Lil, additional, Martine, Lucy, additional, Grégoire, Stéphane, additional, Buteau, Bénédicte, additional, Cabaret, Stéphanie, additional, Rieu, Aurélie, additional, Bermúdez-Humarán, Luis G., additional, Gabrielle, Pierre-Henry, additional, Creuzot-Garcher, Catherine, additional, Berdeaux, Olivier, additional, Acar, Niyazi, additional, and Bringer, Marie-Agnès, additional

Published
2024
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6. OmegaROP-2 prospective study: Expression of placental fatty acid receptors in preterm newborns with retinopathy of prematurity

Author

Carré, Chloé, primary, Acar, Niyazi, additional, Daruich, Alejandra, additional, Grégoire, Stéphane, additional, Martine, Lucy, additional, Buteau, Bénédicte, additional, Aho, Serge, additional, Eid, Petra, additional, Arnould, Louis, additional, Bron, Alain, additional, Driessen, Marine, additional, Kermorvant, Elsa, additional, Simon, Emmanuel, additional, Creuzot-Garcher, Catherine, additional, and Gabrielle, Pierre-Henry, additional

Published
2023
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8. Long-term intake of Lacticaseibacillus helveticus enhances bioavailability of omega-3 fatty acids in the mouse retina

Author

Bringer, Marie-Agnès, Lapaquette, Pierre, Terrat, Sébastien, Proukhnitzky, Lil, Martine, Lucy, Grégoire, Stéphane, Buteau, Bénédicte, Rieu, Aurélie, Bermúdez-Humarán, Luis, Gabrielle, Pierre Henry, Creuzot‐Garcher, Catherine, Berdeaux, Olivier, Acar, Niyazi, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Dijon, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Bibliothèques universitaires [Université de Bourgogne] (BU), Université de Bourgogne (UB), Université Bourgogne Franche-Comté [COMUE] (UBFC), Service d'Ophtalmologie (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Agence Nationale de la Recherche, INRAE, French 'Investissements d’Avenir' program, project ISITE-BFC, Conseil Régional de Bourgogne, Franche-Comté [PARI grant], FEDER (European Regional Development Fund) and Institut Carnot Qualiment [grant INPROBIAUS]., ANR-11-LABX-0021,Lipstic,Lipoprotéines et santé : prévention et traitement des maladies inflammatoires non vasculaires et du cancer(2011), ANR-15-IDEX-0003,BFC,ISITE ' BFC(2015), European Project: FEDER, Julien, Sabine, Laboratoires d'excellence - Lipoprotéines et santé : prévention et traitement des maladies inflammatoires non vasculaires et du cancer - - Lipstic2011 - ANR-11-LABX-0021 - LABX - VALID, ISITE ' BFC - - BFC2015 - ANR-15-IDEX-0003 - IDEX - VALID, and FEDER - FEDER - INCOMING

Subjects
Microbiota, Probiotic, Retina, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, Plasma, Docosahexaenoic acid, Metabolism, Liver, [SDV.MHEP.OS] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, 22 Fatty acids, Lactic acid bacteria, [SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Phospholipids
Abstract

Omega-3 (n-3) polyunsaturated fatty acids (PUFAs), particularly docosahexaenoic acid (DHA), are required for the structure and function of the retina. They could also help to prevent or delay the development of retinopathies. Given the accumulating evidence showing the role of gut microbiota in regulating retinal physiology and host lipid metabolism, we evaluated the potential of long-term dietary supplementation with the Gram-positive bacterium Lacticaseibacillus helveticus strain VEL12193 to modulate the retinal n-3 PUFA content. A set of complementary approaches was used to study the impact of such a supplementation on the gut microbiota and host lipid/fatty acid (FA) metabolism. L. helveticus-supplementation was associated with a decrease in retinal saturated FAs (SFAs) and monounsaturated FAs (MUFAs) as well as an increase in retinal n-3 and omega-6 (n-6) PUFAs. Interestingly, supplementation with L. helveticus enriched the retina in C22:5n-3 (docosapentaenoic acid, DPA), C22:6n-3 (DHA), C18:2n-6 (linoleic acid, LA) and C20:3n-6 (dihomo gamma-linolenic acid, DGLA). Long-term consumption of L. helveticus also modulated gut microbiota composition and some changes in OTUs abundance correlated with the retinal FA content. This study provides a proof of concept that targeting the gut microbiota could be an effective strategy to modulate the retinal FA content, including that of protective n-3 PUFAs, thus opening paths for the design of novel preventive and/or therapeutical strategies for retinopathies.

Published
2023

9. Effects of topical docosahexaenoic acid on postoperative fibrosis in an animal model of glaucoma filtration surgery

Author

Carré, Chloé, primary, Baudin, Florian, additional, Buteau, Bénédicte, additional, Martine, Lucy, additional, Grégoire, Stéphane, additional, Vasku, Glenda, additional, Berdeaux, Olivier, additional, Béduneau, Arnaud, additional, Pellequer, Yann, additional, Jamoussi, Jasmine, additional, Desrumeaux, Catherine, additional, Aho, Serge, additional, Bron, Alain‐Marie, additional, Acar, Niyazi, additional, Creuzot‐Garcher, Catherine, additional, and Gabrielle, Pierre Henri, additional

Published
2022
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11. Plasmalogens Regulate Retinal Connexin 43 Expression and Müller Glial Cells Gap Junction Intercellular Communication and Migration

Author

Karadayi, Rémi, Mazzocco, Julie, Leclere, Laurent, Buteau, Bénédicte, Gregoire, Stéphane, Belloir, Christine, Koudsi, Mounzer, Bessard, Pauline, Bizeau, Jean-Baptiste, Dubus, Elisabeth, Fenech, Claire, Briand, Loïc, Bretillon, Lionel, Bron, Alain M., Fioramonti, Xavier, and Acar, Niyazi

Subjects
Cell Biology, Developmental Biology
Published
2022
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12. Impact of dietary fatty acids on autophagy in the retina

Author

Bizeau, Jean-Baptiste, Buteau, Bénédicte, Martine, Lucy, Grégoire, Stéphane, Leclère, Laurent, Dubus, Elisabeth, Bron, Alain Marie, Acar, Niyazi, Bringer, Marie-Agnès, and Bringer, Marie-Agnès

Subjects
[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition
Published
2022

14. Effects of topical docosahexaenoic acid on postoperative fibrosis in an animal model of glaucoma filtration surgery.

Author

Carré, Chloé, Baudin, Florian, Buteau, Bénédicte, Martine, Lucy, Grégoire, Stéphane, Vasku, Glenda, Berdeaux, Olivier, Béduneau, Arnaud, Pellequer, Yann, Jamoussi, Jasmine, Desrumeaux, Catherine, Aho, Serge, Bron, Alain‐Marie, Acar, Niyazi, Creuzot‐Garcher, Catherine, and Gabrielle, Pierre Henri

Subjects
FILTERING surgery, DOCOSAHEXAENOIC acid, SMAD proteins, RATTUS norvegicus, TONOMETERS, ANIMAL models in research, WESTERN immunoblotting
Abstract

Purpose: The aim of this study was to evaluate docosahexaenoic acid (DHA) as a potential antifibrotic agent after glaucoma filtration surgery (GFS) in rats. Methods: A total of 36 10‐week‐old Brown Norway rats underwent GFS. Animals were equally divided into three groups: a control group, a DHA group and a mitomycin C (MMC) group. Intraocular pressure (IOP) was measured using a dynamic rebound tonometer, and a photograph of the surgical site was taken on days 1, 3, 7, 10, 14 and 17. The incorporation of DHA into fibroblasts was evaluated by gas chromatography. The expression of alfa‐smooth muscle actin (α‐SMA) and Smad proteins was assessed by Western blotting. Results: IOP decreased after surgery in animals from the three groups on day 1 after surgery. Over time, IOP remained lower in the DHA and MMC groups than in the control group (median [interquartile range] 8.0 [7.0–8.0] and 8.0 [7.3–8.0] mmHg vs. 9.0 [8.0–9.0] mmHg, respectively; p < 0.001). Bleb area in the DHA and MMC groups remained larger than that of the control group from day 7 to day 14 (3.9 [2.9–5.2] and 3.5 [2.3–4.4] mm2 vs. 2.3 [2.0–2.8] mm2, respectively; p = 0.0021). We did not observe any change in DHA concentrations in the fibroblasts of the DHA group compared with the other groups. Conclusion: The impact of DHA on IOP and bleb area was similar to that of MMC. The mechanisms of action of DHA in rat eye fibroblasts deserve further investigation. [ABSTRACT FROM AUTHOR]

Published
2023
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17. Age-Related Changes in the Gut Microbiota Modify Brain Lipid Composition

Author

Albouery, Mayssa, primary, Buteau, Bénédicte, additional, Grégoire, Stéphane, additional, Cherbuy, Claire, additional, Pais de Barros, Jean-Paul, additional, Martine, Lucy, additional, Chain, Florian, additional, Cabaret, Stéphanie, additional, Berdeaux, Olivier, additional, Bron, Alain M., additional, Acar, Niyazi, additional, Langella, Philippe, additional, and Bringer, Marie-Agnès, additional

Published
2020
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18. Gap junction intercellular communication (GJIC) is regulated by plasmalogens in retinal macroglial cells

Author

Karadayi, Rémi, Leclere, Laurent, Koudsi, Mounzer, Bessard, Pauline, Buteau, Bénédicte, Grégoire, Stéphane, Fenech, Claire, Fioramonti, Xavier, Mazzocco, Julie, Bron, Alain M., Acar, Niyazi, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Université Bourgogne Franche-Comté [COMUE] (UBFC), Nutrition et Neurobiologie intégrée (NutriNeuro), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Ecole nationale supérieure de chimie, biologie et physique, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Projet 2016-EQ6-08, and ProdInra, Migration

Subjects
gap junction, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, retina, calcium, [SDV.MHEP.OS] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, glia, [SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs, plasmalogens, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2019

19. The ApoB100,LDLR-/- mouse model as an experimental model to study aging of the retina: focus on autophagy, inflammation and oxidative stress

Author

Bringer, Marie-Agnès, Albouery, Mayssa, Buteau, Bénédicte, Maire, Marie-Annick, Bron, Alain M., Creuzot Garcher, Catherine, Acar, Niyazi, Brétillon, Lionel, ProdInra, Migration, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Université Bourgogne Franche-Comté [COMUE] (UBFC), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Agence Nationale de la Recherche [ANR11-LABX-0021-01], Conseil Regional Bourgogne, Franche-Comte [PARI grant], FEDER (European Funding for Regional Economical Development), and Fondation de France/Fondation de l'oeil.

Subjects
[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, [SDV.MHEP.OS] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2019

20. Characterization of cholesterol metabolism in an experimental model of glaucoma in rats

Author

Masson, Elodie A.Y., Léger Charnay, Elise, Gambert Nicot, Ségolène, Martine, Lucy, Buteau, Bénédicte, Maire, Marie-Annick, Gigot, Vincent, Bron, Alain, Creuzot Garcher, Catherine, Acar, Niyazi, Brétillon, Lionel, ProdInra, Migration, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Université Bourgogne Franche-Comté [COMUE] (UBFC), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Conseil Regional Bourgogne, Franche-Comte [PARI grant], FEDER (European Funding for Regional Economical Development), and Fondation de France/Fondation de l'oeil.

Subjects
[SDV.MHEP.EM] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism, glaucoma, [SDV.MHEP.OS] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, cholesterol, lipids (amino acids, peptides, and proteins), sense organs, [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism, [SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs
Abstract

International audience; Glaucoma is a neurodegenerative pathology characterized by retinal ganglion cell death. Cholesterol homeostasis disruption has been implicated in several neurodegenerative diseases such as Alzheimer and Huntington. However, its specific role in the retina and association with glaucoma remain unclear. The aim of this study was to characterize the modifications of cholesterol metabolism occurring during an experimental model of glaucoma in rats. Eight-weeks old male rats were subjected to laser photocoagulation of episcleral veins, limbus and trabecular meshwork to elevate intraocular pressure. At a short (3 days) and a long (2 months) time period post-laser, the retinal levels of sterols and oxysterols were measured by gas chromatography. The expression of various genes implicated in cholesterol metabolism was assayed by RT-qPCR. Statistical significance was calculated using the paired Wilcoxon test (n=8). At 3 days post-laser, the levels of cholesterol precursors, desmosterol and lathosterol, were strongly increased (x1.6 between laser-treated and contralateral retinas, p=0.008 and x3, p=0.02, respectively) while cholesterol levels were slightly increased (x 1.3, p=0.008). We did not detect any change in the levels of 24S-hydroxycholesterol, a major elimination form of cholesterol in retinal ganglion cells. On the contrary, the expression of ABCA1, a transporter regulating cholesterol efflux, as well as LXR, a transcription factor activating hypocholesterolemic pathways, was increased. The expression of LDL receptor, regulating cholesterol uptake, as well as SREBP2, a transcription factor activating hypercholesterolemic pathways, was decreased. At 2 months post-laser, cholesterol precursor levels were decreased. Interestingly, we also measured a significant increase in the expression of CYP27A1 (x1.8, p=0.02), an enzyme implicated in cholesterol efflux and in the levels of its product 27-hydroxycholesterol (x1.6, p=0.02). Our observations suggest that cholesterol metabolism disturbances are associated with glaucoma. Indeed, hypocholesterolemic mechanisms are induced in the rat glaucoma model in order to counteract the activation of cholesterol biosynthetic pathways consecutive to intraocular pressure elevation. Moreover, our results suggest that CYP27A1 might play an important role in the regulation of cholesterol metabolism in the retina.

Published
2019

21. Impact of a reduction of plasmalogen levels on Muller cell metabolism

Author

Pallot, Charlotte, Mazzocco, Julie, Grégoire, Stéphane, Leclere, Laurent, Buteau, Bénédicte, Bron, Alain, Creuzot Garcher, Catherine, Brétillon, Lionel, Acar, Niyazi, Ophtalmologie, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Service d'Ophtalmologie (CHU de Dijon), OEil, nutrition et signalisation cellulaire [CSGA], Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ) -Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), National Institute for Research on Agronomy (INRA) Regional Council of Burgundy European Regional Development Fund (FEDER), Assoc Res Vis & Ophthalmol, Ophthalmologie, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), Université Bourgogne Franche-Comté [COMUE] (UBFC), Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), ProdInra, Archive Ouverte, Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Université Bourgogne Franche-Comté ( UBFC ), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), and université de Bourgogne, CSGA

Subjects
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, enfant prématuré, cecite, métabolisme cellulaire, acide gras oméga 3, premature infant, [SDV.MHEP.OS] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [ SDV.MHEP.OS ] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, Ophtalmology, [ SDV.MHEP ] Life Sciences [q-bio]/Human health and pathology, [SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, ComputingMilieux_MISCELLANEOUS, blindness
Abstract

Purpose : Retinopathy of Prematurity (ROP) is the leading cause of blindness in premature children resulting from abnormal vascular development. Previous studies have shown that omega-3 polyunsaturated fatty acids (PUFAs) can modulate physiologic and pathologic angiogenesis. Data from our laboratory suggest that plasmalogens, that are phospholipids that concentrate omega-3 PUFAs, are involved in the regulation of post-natal retinal vascular development through the action of a calcium-independent phospholipase A2 (iPLA2) (Saab et al PLoSONE 2014). In the retina, Müller cells are known to play crucial functions in retinal angiogenesis as well as to be rich in plasmalogens and to express phospholipases. The aim of this study is to investigate the roles of plasmalogens and omega-3 PUFAs on Müller cell metabolism.Methods : Primary cultures of rat Müller cells were treated with siRNA against the major enzyme for plasmalogen biosynthesis (DHAP-AT) and/or with a chemical inhibitor of iPLA2, bromoenol lactone (BEL). Lipid profile of cells was determined by gas chromatography, cell proliferation by Ki67 assay and cell migration was evaluated by videomicroscopy. The expression of p38, ERK, JNK in their non-phosphorylated and phosphorylated forms was determined by western blotting.Results : The use of siRNA against DHAP-AT led to a 50%-diminution of plasmalogens levels in cells. Decreasing the cell levels of plasmalogens and/or inhibiting iPLA2 had no impact on cellular viability. A reduction of the ability of cells to migrate was observed in samples treated with siRNA against DHAP-AT at 72 hours. The inhibition of the expression of DHAP-AT led to an increase in the rate of phosphorylation of ERK. No effect of BEL was observed on these parameters.Conclusions : Plasmalogens seem to initiate a MAPK-dependent signalling cascade in retinal Müller cells that might impact cell phenotype. Alteration of Müller cell metabolism seems to be the consequence of a diminution of plasmalogens levels following siRNA treatment rather than that of an inhibition of iPLA2 by BEL.This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

Published
2016

22. Plasmalogens and cell-cell communication between retinal glial cells

Author

Mazzocco, Julie, Pallot, Charlotte, Buteau, Bénédicte, Grégoire, Stéphane, Leclere, Laurent, Bron, Alain, Creuaot-Garcher, Catherine, Brétillon, Lionel, Acar, Niyazi, OEil, nutrition et signalisation cellulaire [CSGA], Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Service d'Ophtalmologie (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), French National Institute for Agricultural Research (INRA) National Center for Scientific Research (CNRS) Regional Council of Burgundy European Regional Development Fund (FEDER) University of Burgundy, Assoc Res Vis & Ophthalmol, Université Bourgogne Franche-Comté [COMUE] (UBFC), Ophthalmology, Radboud University Medical Center [Nijmegen], Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ) -Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Université Bourgogne Franche-Comté ( UBFC ), université de Bourgogne, CSGA, and Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)

Subjects
retinal glial cells, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Organes des sens, [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition, rétine, Sensory Organs, glial cell, [ SDV.MHEP.OS ] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [ SDV.MHEP ] Life Sciences [q-bio]/Human health and pathology, Alimentation et Nutrition, Food and Nutrition, ophtalmology, cellule gliale, [SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, ComputingMilieux_MISCELLANEOUS, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.; Purpose: Plasmalogens (or ether-lipids) are a particular class of glycerophospholipids characterized by the presence of a vinyl-ether bond at the sn-1 position of the glycerol backbone and by the preferential esterification of polyunsaturated fatty acids (PUFAs) at the sn-2 position. Plasmalogens are found at high concentrations in retinal macroglial cells such as Müller cells and astrocytes. Müller cells and astrocytes are known to communicate by the way of intracellular calcium waves and gap junctions. Connexin 43 is the major protein of these communicating junctions. The aim of this study was to determine the effect of plasmalogens depletion on communication between astrocytes and Müller cells. Methods: Primary retinal Müller cells were isolated from eyes of Wistar rats aged from 9 days whereas astrocytes were obtained from cortex of 21-days old animals. SiRNA against DHAPA-AT -the key enzyme of plasmalogen biosynthesis- were used to decrease the cell content in plasmalogen. After lipid extraction, the concentrations of plasmalogens in cell membranes were monitored by gas chromatography coupled to flame ionization detection. Communication between astrocytes and Müller cells was studied by calcium imaging. Changes in the expression cellular proteins, including connexin 43, were evaluated by proteomics and western-blotting. Results: Lipidomic analyses confirmed that plasmalogens are concentrated in retinal macroglial cells (17% and 13% of total phospholipids for Müller cells and astrocytes, respectively) when compared to the entire retina (7% of total phospholipids). SiRNA against DHAP-AT led to a diminution of plasmalogen content of more than 50%. Decreasing the concentrations of plasmalogens in membranes of glial cells impacted cell-cell communication through decrease in the expression of connexion 43 by more than 50%. Western-blot analyses showed modifications in the expression/phosphorylation of MAPK proteins. Conclusions: Plasmalogens may influence cell-cell communication between retinal Müller cells and astrocytes. As retinal macroglial cells play crucial roles in preserving neuronal integrity and in proper vascular development, modifications of retinal plasmalogen levels are likely to be at the origin of many retinal diseases.

Published
2016

23. Characterization of plasmalogens in retinal glial cells

Author

Mazzocco, Julie, Buteau, Bénédicte, Leclere, Laurent, Creuzot Garcher, Catherine P., Bron, Alain, Bretillon, Lionel, Acar, Niyazi, Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), ProdInra, Archive Ouverte, and Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, [SDV.MHEP.OS] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [ SDV.MHEP ] Life Sciences [q-bio]/Human health and pathology, [ SDV.MHEP.OS ] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition, [SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

Purpose: Plasmalogens (or ether-lipids) constitute a particular class of glycerophospholipids that are characterized by the presence of a vinyl-ether bond at the sn-1 position of the glycerol backbone and by the preferential esterification of polyunsaturated fatty acids (PUFAs) at the sn-2 position. Although their exact functions remain enigmatic, previous data from our laboratory suggest that plasmalogens play crucial functions during retinal vessel development by affecting astrocyte template formation (Saab et al PLoSONE 2014;9(6):e101076). The aim of this study was to better characterize plasmalogens in the retina and to determine which cells are responsible for plasmalogens production within the populations of retinal glial cells.Methods: Retinal Müller cells and astrocytes were isolated from eyes of Wistar rats aged from 12 to 21 days and cultured in DMEM medium. The presence of dihydroxyacetone-phosphate acyltransferase (DHAP-AT), the key enzyme of plasmalogen biosynthesis, was evaluated by western-blotting in each cell type. After lipid extraction, the cellular content of plasmalogens was determined by gas chromatography coupled to flame ionization detection (GC-FID). Furthermore, DHAP-AT immunostainings were performed on cultured cells as well as on retinal sections from eyes of C57BL/6 mice.Results: Immunohistochemistry revealed that DHAP-AT protein was present in the inner and the outer plexiform layers, as well as in photoreceptor inner segments. Immunostainings and western-blot analyses on primary cultures showed that DHAP-AT was highly expressed in Müller cells. Lipid analyses revealed that the levels of plasmalogens were higher in Müller cells than in astrocytes and the whole retina.Conclusions: These results highlight the differences between astrocytes and Müller cells in plasmalogen levels and key-enzyme in plasmalogen synthesis. Plasmalogens-dependent signalling pathways in the retina may therefore be specifically initiated in Müller cells. Further studies are needed to characterize the cell pathways activated by plasmalogens catabolism in Müller and neighbouring cells.

Published
2015

24. Involvement of plasmalogens and iPLA2 in the control of post-natal retinal vascular development in mice

Author

Acar, Niyazi, SAAB, Sara, Buteau, Bénédicte, Leclere, Laurent, Bron, Alain, Creuzot Garcher, Catherine, Brétillon, Lionel, ProdInra, Migration, Centre des Sciences du Goût et de l'Alimentation (CSGA), and Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, [SDV.MHEP.OS] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs, 698 retinal development, 429 astrocyte, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, 583 lipids
Abstract

International audience; Purpose: The correct development of retinal blood vessels is essential to ensure vision process through sufficient oxygen and nutrient supplies to the retina. It was shown that polyunsaturated fatty acids (PUFAs) could modulate factors involved in tissue vascularization. A congenital deficiency in ether-phospholipids also termed as "plasmalogens" was shown to lead to abnormal ocular vascularization. Because plasmalogens are considered as reservoirs of PUFAs, we wanted to better understand the mechanisms by which plasmalogens may regulate retinal vascular development through the release of PUFAs by a calcium-independent phospholipase A2 (iPLA2). Methods: Retinal vascular development was followed in a mouse model of plasmalogen deficiency (DAPAT mice) as well as in a mouse model of retinal iPLA2 inhibition. Retinas were collected from birth until the age of 21 days. They were either flat-mounted and immuno-labelled to visualize the cells involved in vessel formation, or used to determine the expression of genes involved in inflammation and angiogenesis. Results: The lack of plasmalogens was associated to defects in angiogenic processes during early phases of retinal vascular development. Particularly, the absence of plasmalogens influenced astroglial activity during the primary astrocyte template formation, promoted endothelial cell proliferation during sprouting angiogenesis, and impacted the expression of genes involved in angiopoietin/tie pathways. These early defects led to a disorganized and dysfunctional retinal vascular network at adult age. As mice in which iPLA2 was inhibited exhibited a similar vascular phenotype when compared to DAPAT mice, we suggest that these processes may be mediated by PUFA release from plasmalogens and further signalling through angiopoietin/tie pathways. Conclusions: These data suggest a crucial role of plasmalogens during the formation of retinal vessels.

Published
2014

25. Involvement of plasmalogens and iPLA2 in the control of post-natal retinal vascular development in the mouse

Author

Acar, Niyazi, SAAB, Sarah, Buteau, Bénédicte, Leclere, Laurent, Bron, Alain M., Creuzot Garcher, Catherine P., Bretillon, Lionel, Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Department of Ophthalmology, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), ProdInra, Archive Ouverte, and Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)

Subjects
[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, endocrine system diseases, [SDV.MHEP.OS] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition, [ SDV.MHEP.OS ] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, fungi, [SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

Involvement of plasmalogens and iPLA2 in the control of post-natal retinal vascular development in the mouse. 16. GEM - 10. GERLI lipidomics meeting: from membranes to pathologies

Published
2013

26. Plasmalogens in the regulation of retinal vascular development: design of a mouse model with chemical inhibition of IPLA2

Author

SAAB, Sara, Buteau, Bénédicte, Pasquis, Bruno, Creuzot Garcher, Catherine, Bron, Alain, Bretillon, Lionel, Acar, Niyazi, ProdInra, Archive Ouverte, Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), and Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, [SDV.MHEP.OS] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition, [ SDV.MHEP.OS ] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

Plasmalogens in the regulation of retinal vascular development: design of a mouse model with chemical inhibition of IPLA2. annual meeting of the association-for-research-in-vision-and-ophthalmology (ARVO)

Published
2011

28. Toxicity of Branched-Chain Fatty Acids in Conjunctival Cell Line

Author

Souchier, Maxime, Joffre, Corinne, Leclere, Laurent, Buteau, Bénédicte, Grégoire, Stéphane, Lizard, Gérard, Montange, Thomas, Bron, Alain, Creuzot Garcher, Catherine, Brétillon, Lionel, ProdInra, Migration, Ophthalmology, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), FLAveur, VIsion et Comportement du consommateur (FLAVIC), Institut National de la Recherche Agronomique (INRA)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Université de Bourgogne (UB), Laboratoire des Lipoprotéines humaines et interactions vasculaires, Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre des Sciences du Goût et de l'Alimentation (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), and Association for Research in Vision and Ophthamology (ARVO). USA.

Subjects
lipids, [SDV] Life Sciences [q-bio], conjunctiva, cell death, [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SDV]Life Sciences [q-bio], [SDV.IDA]Life Sciences [q-bio]/Food engineering, apoptosis, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.IDA] Life Sciences [q-bio]/Food engineering
Abstract

International audience; Purpose:: Tear film lipid composition is modified in patients suffering from chronic blepharitis. We previously found that branched chain fatty acids (BCFA) were significantly increased in tears of these patients, suggesting that BCFA may be involved in the disease. The toxicity of different BCFA as potent inducers of apoptosis has already been established in various cell lines but not in the conjunctiva. The aim of this work was to assess the toxic effects of BCFA in cultures of Chang conjunctival cells. Methods:: Chang’s human conjunctival cells were cultured during 4 or 24h with various concentrations of IsoC16 and IsoC20 as BCFA (from 50 to 200µM), by comparison with phytanic acid as a positive control for cytotoxicity or with palmitic acid as a corresponding straight chain fatty acid. The toxicity of fatty acids was assessed by measurement of mitochondrial dehydrogenase activity (MTT test), cell growth, free radical production, cell permeability (propidium iodure), mitochondrial depolarization (Mitotracker) and chromatin condensation (Hoechst). In addition, incorporation of fatty acids in the cells was measured after analysis by gas chromatography of the cells. Results:: IsoC20 and palmitic acid transiently impaired cell viability, as illustrated by the decrease in mitochondrial dehydrogenase activity observed after 4 hours of exposure (p

Published
2007

29. Oxysterols Incorporate in Primary Porcine Retinal Pigment Epithelial Cells and Induce Inflammation and Oxidative Stress

Author

Joffre, Corinne, Leclere, Laurent, Buteau, Bénédicte, Martine, Lucy, Acar, Niyazi, Malvitte, Laura, Bron, Alain, Creuzot Garcher, Catherine, Brétillon, Lionel, ProdInra, Migration, FLAveur, VIsion et Comportement du consommateur (FLAVIC), Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), and Association for Research in Vision and Ophthamology (ARVO). USA.

Subjects
oxidation, inflammation, [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.IDA]Life Sciences [q-bio]/Food engineering, aging, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.IDA] Life Sciences [q-bio]/Food engineering, free radical damage
Abstract

International audience; Purpose: : Aging is associated with an accumulation of cholesterol in the Bruch’s membrane. Cholesterol is prone to undergo oxidation and to generate oxysterols that have cytotoxic properties. The aim of this study was to evaluate the effects of oxysterols on cell viability, oxidative stress and inflammation and to measure oxysterol incorporation in primary porcine retinal pigment epithelial (RPE) cell cultures. Methods: : Porcine RPE cells were incubated with oxysterols (24S–hydroxycholesterol, 24OH ; 25–hydroxycholesterol, 25OH and 7–ketocholesterol, 7K) (100µg, 50µM) for 24 and 48h. Cell viability was evaluated by measuring mitochondrial succinate dehydrogenase (SDH) activity. The content of 24OH, 25OH and 7K was determined in cells and media by gas chromatography. The intracellular formation of reactive oxygen species (ROS) was detected by using the fluorescent probe dichlorofluorescein diacetate (DCFH2–DA). IL–8 was assayed in the media by ELISA and its cellular expression was evaluated by RT–PCR. Results: : Incorporation of 24OH in RPE cells was higher than that of 25OH and 7K (27.9µg vs 10.8 and 6.8µg at 24h; 29.0µg vs 20.8 and 10.3µg at 48h). In the media, 25OH was present in higher amount than 24OH and 7K (31.4µg vs 12.5 and 17.9µg at 24h; 14.1µg vs 7.4 and 10.0µg at 48h). SDH activity increased with 25OH and 24OH compared to the control cells (+35% and +20% with 24h; +25% and +9% with 48h) whereas it decreased for the cells incubated with 7K (–17% with 24h). All oxysterols induced a significant 2–4 fold increase in ROS production compared to the control. Oxysterols induced IL–8 expression (x 2–10) and production in the following decreasing order of magnitude: 25OH (529 pg/mL) > 24OH (211 pg/mL) > 7K (38 pg/mL) > control (4 pg/mL). Conclusions: : These data consistently suggest that the cytotoxicity of 24OH and 25OH in RPE cells may be related to the induction of oxidative and inflammatory reaction that may be involved in the development of age–related diseases.

Published
2006

30. Metabolic Syndrome Triggered by High-Fructose Diet Favors Choroidal Neovascularization and Impairs Retinal Light Sensitivity in the Rat

Author

Thierry, Magalie, primary, Pasquis, Bruno, additional, Acar, Niyazi, additional, Grégoire, Stéphane, additional, Febvret, Valérie, additional, Buteau, Bénédicte, additional, Gambert-Nicot, Ségolène, additional, Bron, Alain M., additional, Creuzot-Garcher, Catherine P., additional, and Bretillon, Lionel, additional

Published
2014
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32. La réponse non spécifique des leucocytes de truite arc-en-ciel au virus de la septicémie hémorragique virale : étude systématique des modifications du transcriptome

Author

O'farrell, C, Vaghefi, N, Massin, Pascale, Cantonnet, Monique, Buteau, Bénédicte, Boudinot, Pierre, Benmansour, Abdenour, Inconnu, Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), and Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2003

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