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3. Cereal genes similar to Snf2 define a new subfamily that includes human and mouse genes

Author

Yan, L, Echenique, V, Busso, C, SanMiguel, P, Ramakrishna, W, Bennetzen, J, Harrington, S, and Dubcovsky, J

Subjects
Genetics, Human Genome, Biotechnology, Aetiology, 2.1 Biological and endogenous factors, Adenosine Triphosphatases, Alternative Splicing, Amino Acid Sequence, Animals, Arabidopsis Proteins, Carrier Proteins, Chromosome Mapping, Chromosomes, Chromosomes, Artificial, Bacterial, Cloning, Molecular, DNA Helicases, DNA Primers, DNA-Binding Proteins, Gene Expression Regulation, Plant, Genes, Plant, Hordeum, Humans, Mice, Molecular Sequence Data, Nuclear Proteins, Oryza, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction, Sequence Homology, Amino Acid, Transcription Factors, Triticum, SNF2 family, alternative splicing, comparative genomics, wheat, vernalization gene Vrn2, Plant Biology, Genetics & Heredity, Plant Biology & Botany
Abstract

Genes from the SNF2 family play important roles in transcriptional regulation, maintenance of chromosome integrity and DNA repair. This study describes the molecular cloning and characterization of cereal genes from this family. The predicted proteins exhibit a novel C-terminal domain that defines a new subfamily designated SNF2P that includes human and mouse proteins. Comparison between genomic and cDNA sequences showed that cereal Snf2P genes consisted of 17 exons, including one only 8 bp long. Two barley alleles differed by the presence of a 7.7-kb non-LTR retrotransposon in intron 6. An alternative annotation of the orthologous Arabidopsis gene would improve its similarity with the other members of the subfamily. Intron 2 was not spliced out in approximately half of the rice Snf2P mRNAs present in leaves, resulting in a premature stop codon. Transcripts from the barley and wheat Snf2P genes were found in apexes, leaves, sheaths, roots and spikes. The Snf2P genes exist as single copies on wheat chromosome arm 5A(m)L and in the colinear regions on barley chromosome arm 4HL and rice chromosome 3. High-density genetic mapping and RT-PCR suggest that Snf2P is not a candidate gene for the tightly linked vernalization gene Vrn2.

Published
2002

29. Potencial do óleo essencial de erva-luísa (Aloysia citriodora Palau) no controle de Fusarium sp. in vitro

Author

FREDDO, Á.R., MAZARO, S.M., BORIN, M.S.R., BUSSO, C., CECHIN, F.E., ZORZZI, I.C., DALACOSTA, N.L., and LEWANDOWSKI, A.

Subjects
Verbenaceae, Beta vulgaris L, fitopatologia, Beta vulgarisL, plantas medicinais, phytopathology, medicinal plants
Abstract

RESUMO O presente trabalho teve como objetivo principal avaliar o potencial do uso do óleo essencial de Aloysia citriodora no controle in vitro de Fusarium sp., isolado de plântulas de beterraba infectadas com o mesmo. O trabalho foi realizado por meio de dois experimentos: um sobre o efeito do óleo essencial no crescimento micelial, e outro sobre o efeito na germinação de conídios do fungo. No primeiro trabalho, avaliou-se em delineamento inteiramente ao acaso, o efeito das concentrações 0,0155%; 0,0315%; 0,0625%; 0,125%; 0,250% e 0,500% do óleo essencial de A. citriodora em placas de Petri® com meio de cultivo BDA, mais a testemunha, com meio BDA puro. Cada placa foi considerada uma repetição, as quais foram incubadas a 24ºC±1ºC e submetidas a fotoperíodo de doze horas. Avaliou-se o crescimento radial do patógeno em função do crescimento micelial do patógeno quando este atingia as bordas da primeira placa. No segundo experimento, as mesmas concentrações foram testadas, nas mesmas condições de incubação, no entanto, em lâminas de microscopia com meio BD. Utilizou-se delineamento inteiramente ao acaso, sendo considerada como unidade experimental cada lâmina utilizada. Avaliou-se 24 horas após a incubação, em microscópio óptico, a germinação dos primeiros vinte conídios visualizados a partir do canto esquerdo superior para o direito. Os resultados do segundo experimento foram expressos em porcentagem de germinação de conídios. Os resultados obtidos permitiram concluir que este óleo essencial possui efeito fungistático e fungicida sobre o crescimento micelial e na germinação de conídios de Fusarium sp.. Além disso este efeito é maior em função do aumento da concentração do óleo essencial. ABSTRACT This study aimed to evaluate the potential use of Aloysia citriodora essential oilisolated from beet seedlings infected with it. The study was performed by conducting two experiments evaluating the effect of the essential oil on mycelial growth and fungus conidia germination. In the first study, in a completely randomized design, the effect concentrations (0.0155%; 0.0315%; 0.0625%; 0.125%; 0.250% and 0.500%) of essential oil of A. citriodorawas evaluated on Petri® dish with a PDA cultivation medium, plus the control, with half pure PDA. Each plate was taken as a repeat, and incubated at 24°C±1°C and a photoperiod of twelve hours. The radial growth of the pathogen, when the first plate was hit by the mycelial growth of the pathogen on its edges was evaluated. In the second experiment, the same concentrations were tested under the same incubationconditions, however, on microscope slides with half PD medium. The design was used completely randomized, each microscope slide used was considered as one experimental unit. Germination of the first 2 conidia strains, viewed from the upper left to the right was evaluated24 hours after incubation, using an optical microscope. The results of the second experiment were expressed as a percentage of conidia germination. The results obtained showed that this essential oil has fungistatic and fungicidal effect on the mycelial growth and at the conidia germination of Fusarium sp., which has a greater effect with increasing concentrations.

Published
2016

38. Plant growth and survival of five introduced and two native/naturalized perennial grass genotypes exposed to two defoliation managements in arid Argentina

Author

Torres, Y. A., primary, Busso, C. A., additional, Montenegro, O. A., additional, Ithurrart, L., additional, Giorgetti, H. D., additional, Rodríguez, G. D., additional, Bentivegna, D., additional, Brevedan, R. E., additional, Fernández, O. A., additional, Mujica, M. M., additional, Baioni, S. S., additional, Entío, L., additional, Fioretti, M., additional, and Tucat, G., additional

Published
2013
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40. SESSION 47: ART - IMPACT OF LEGISLATION

Author

Delbaere, A., primary, Laruelle, C., additional, Place, I., additional, Devreker, F., additional, Englert, Y., additional, Maggiulli, R., additional, Capalbo, A., additional, Colamaria, S., additional, Giuliani, M., additional, Baroni, E., additional, Sapienza, F., additional, Rienzi, L., additional, Ubaldi, F. M., additional, Yildiz, G., additional, Candan, Z. N., additional, Avcil, F., additional, Ozden, H., additional, Uslu, H., additional, Karaman, Y., additional, Duarte Filho, O. B., additional, Busso, C. E., additional, Tso, L. O., additional, Mizrahi, F. E., additional, Antunes Junior, N., additional, Tognotti, E., additional, Busso, N. E., additional, Soares, J. B., additional, Knez, J., additional, Kovacic, B., additional, Reljic, M., additional, Gavric-Lovrec, V., additional, Kovac, V., additional, and Vlaisavljevic, V., additional

Published
2012
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43. The non-ergot derived dopamine agonist quinagolide in prevention of early ovarian hyperstimulation syndrome in IVF patients: a randomized, double-blind, placebo-controlled trial

Author

Busso, C., primary, Fernandez-Sanchez, M., additional, Garcia-Velasco, J. A., additional, Landeras, J., additional, Ballesteros, A., additional, Munoz, E., additional, Gonzalez, S., additional, Simon, C., additional, Arce, J.-C., additional, and Pellicer, A., additional

Published
2010
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45. Plant growth and survival of five introduced and two native/naturalized perennial grass genotypes exposed to two defoliation managements in arid Argentina.

Author

Torres, Y. A., Busso, C. A., Montenegro, O. A., Ithurrart, L., Giorgetti, H. D., Rodríguez, G. D., Bentivegna, D., Brevedan, R. E., Fernández, O. A., Mujica, M. M., Baioni, S. S., Entío, L., Fioretti, M., and Tucat, G.

Subjects
*PLANT growth, *PERENNIALS, *GRASS genetics, *DEFOLIATION, *CULTIVARS, *FORAGE plants, *ARID regions
Abstract

The field performance of the native Pappophorum vaginatum, the naturalized Eragrostis curvula and various cultivars of the introduced Achnatherum hymenoides and Leymus cinereus was evaluated as potential forage resources in rangelands of arid Argentina during the warm seasons of 2007/2008 and 2008/2009. Plants of these grass species, obtained from seeds, were transplanted to the field in 2006, when they were 1 year old. During the study years, there were two defoliation managements: plants of all study genotypes either remained undefoliated (controls) or were defoliated twice a year during spring at 5 cm stubble height. Despite tiller number being lower ( P < 0·05) on defoliated than on undefoliated plants, and total leaf length per unit basal area being similar ( P > 0·05) between defoliation managements by mid-spring, there were no differences ( P > 0·05) in dry weight production between defoliated and undefoliated plants in all genotypes at the end of the second growing season. Plants of one or more of the introduced genotypes showed a similar ( P > 0·05) or greater ( P < 0·05), but not lower, tiller number per plant and per square centimetre, daughter tiller production, total leaf length and dry weight production per unit basal area than the native species at the end of the first and/or second growing seasons. These morphological variables were similar ( P > 0·05) or greater ( P < 0·05) in the native than in the naturalized genotype. Plant survival, however, was lower ( P < 0·05, overall mean = 20%) in the introduced than in the native (>70%) or naturalized (>39%) genotypes at the end of the first or second growing seasons. [ABSTRACT FROM AUTHOR]

Published
2014
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