17 results on '"Burmester, Marion"'
Search Results
2. Caco-2/HT29-MTX co-cultured cells as a model for studying physiological properties and toxin-induced effects on intestinal cells
- Author
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Hoffmann, Pascal, primary, Burmester, Marion, additional, Langeheine, Marion, additional, Brehm, Ralph, additional, Empl, Michael T., additional, Seeger, Bettina, additional, and Breves, Gerhard, additional
- Published
- 2021
- Full Text
- View/download PDF
3. Modulation of Intestinal Phosphate Transport in Young Goats Fed a Low Phosphorus Diet
- Author
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Behrens, Joie L., Schnepel, Nadine, Hansen, Kathrin, Hustedt, Karin, Burmester, Marion, Klinger, Stefanie, Breves, Gerhard, and Muscher-Banse, Alexandra S.
- Subjects
Male ,Duodenum ,Goats ,PiT1 ,Phosphorus ,Sodium-Phosphate Cotransporter Proteins ,Article ,lcsh:Chemistry ,Calcitriol ,Intestinal Absorption ,phosphate homeostasis ,lcsh:Biology (General) ,lcsh:QD1-999 ,Ileum ,Intestinal Elimination ,PiT2 ,Animals ,Homeostasis ,Phosphorus, Dietary ,Intestinal Mucosa ,NaPiIIb ,intestine ,lcsh:QH301-705.5 - Abstract
The intestinal absorption of phosphate (Pi) takes place transcellularly through the active NaPi-cotransporters type IIb (NaPiIIb) and III (PiT1 and PiT2) and paracellularly by diffusion through tight junction (TJ) proteins. The localisation along the intestines and the regulation of Pi absorption differ between species and are not fully understood. It is known that 1,25-dihydroxy-vitamin D3 (1,25-(OH)2D3) and phosphorus (P) depletion modulate intestinal Pi absorption in vertebrates in different ways. In addition to the apical uptake into the enterocytes, there are uncertainties regarding the basolateral excretion of Pi. Functional ex vivo experiments in Ussing chambers and molecular studies of small intestinal epithelia were carried out on P-deficient goats in order to elucidate the transepithelial Pi route in the intestine as well as the underlying mechanisms of its regulation and the proteins, which may be involved. The dietary P reduction had no effect on the duodenal and ileal Pi transport rate in growing goats. The ileal PiT1 and PiT2 mRNA expressions increased significantly, while the ileal PiT1 protein expression, the mid jejunal claudin-2 mRNA expression and the serum 1,25-(OH)2D3 levels were significantly reduced. These results advance the state of knowledge concerning the complex mechanisms of the Pi homeostasis in vertebrates.
- Published
- 2021
4. The effect of ischaemic postconditioning on mucosal integrity and function in equine jejunal ischaemia
- Author
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Verhaar, Nicole, primary, Breves, Gerhard, additional, Hewicker‐Trautwein, Marion, additional, Pfarrer, Christiane, additional, Rohn, Karl, additional, Burmester, Marion, additional, Schnepel, Nadine, additional, Neudeck, Stephan, additional, Twele, Lara, additional, and Kästner, Sabine, additional
- Published
- 2021
- Full Text
- View/download PDF
5. The effect of ischaemic postconditioning on mucosal integrity and function in equine jejunal ischaemia
- Author
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Verhaar, Nicole, primary, Breves, Gerhard, additional, Hewicker-Trautwein, Marion, additional, Pfarrer, Christiane, additional, Rohn, Karl, additional, Burmester, Marion, additional, Schnepel, Nadine, additional, Neudeck, Stephan, additional, Twele, Lara, additional, and Kaestner, Sabine, additional
- Published
- 2020
- Full Text
- View/download PDF
6. The effect of ischaemic postconditioning on mucosal integrity and function in equine jejunal ischaemia.
- Author
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Verhaar, Nicole, Breves, Gerhard, Hewicker‐Trautwein, Marion, Pfarrer, Christiane, Rohn, Karl, Burmester, Marion, Schnepel, Nadine, Neudeck, Stephan, Twele, Lara, and Kästner, Sabine
- Abstract
Summary: Background: Ischaemic postconditioning (IPoC) has been shown to ameliorate ischaemia reperfusion injury in different species and tissues. Objectives: To assess the feasibility of IPoC in equine small intestinal ischaemia and to assess its effect on histomorphology, electrophysiology and paracellular permeability. Study design: Randomised in vivo experiment. Methods: Experimental jejunal ischaemia was induced for 90 min in horses under general anaesthesia. In the control group (C; n = 7), the jejunum was reperfused without further intervention. In the postconditioning group (IPoC; n = 7), reocclusion was implemented following release of ischaemia by clamping the mesenteric vessels in three cycles of 30 seconds. This was followed by 120 minutes of reperfusion in both groups. Intestinal microperfusion and oxygenation was measured during IPoC using spectrophotometry and Doppler flowmetry. Histomorphology and histomorphometry of the intestinal mucosa were assessed. Furthermore, electrophysiological variables and unidirectional flux rates of 3H‐mannitol were determined in Ussing chambers. Western blot analysis was performed to determine the tight junction protein levels of claudin‐1, claudin‐2 and occludin in the intestinal mucosa. Comparisons between the groups and time points were performed using a two‐way repeated measures analysis of variance (ANOVA) or non‐parametric statistical tests for the ordinal and not normally distributed data (significance P <.05). Results: IPoC significantly reduced intestinal microperfusion during all clamping cycles yet affected oxygen saturation only during the first cycle. After reperfusion, Group IPoC showed significantly less mucosal villus denudation (mean difference 21.5%, P =.02) and decreased mucosal‐to‐serosal flux rates (mean difference 15.2 nM/cm2/h, P =.007) compared to Group C. There were no significant differences between the groups for the other tested variables. Main limitations: Small sample size, long‐term effects were not investigated. Conclusions: Following IPoC, the intestinal mucosa demonstrated significantly less villus denudation and paracellular permeability compared to the untreated control group, possibly indicating a protective effect of IPoC on ischaemia reperfusion injury. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
7. The application of rumen simulation technique (RUSITEC) for studying dynamics of the bacterial community and metabolome in rumen fluid and the effects of a challenge with Clostridium perfringens
- Author
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Wetzels, Stefanie U., Eger, Melanie, Burmester, Marion, Kreienbrock, Lothar, Abdulmawjood, Amir, Pinior, Beate, Wagner, Martin, Breves, Gerhard, and Mann, Evelyne
- Subjects
Metabolic Processes ,Rumen ,Clostridium perfringens ,Prevotella ,Marine and Aquatic Sciences ,lcsh:Medicine ,Microbial Genomics ,In Vitro Techniques ,Pathology and Laboratory Medicine ,Biochemistry ,Microbiology ,Tandem Mass Spectrometry ,Limnology ,Medicine and Health Sciences ,Metabolites ,Genetics ,Metabolomics ,Animals ,lcsh:Science ,Microbial Pathogens ,Bacteria ,Microbiota ,Gut Bacteria ,Ecology and Environmental Sciences ,lcsh:R ,Organisms ,Biology and Life Sciences ,Genomics ,Bacterial Pathogens ,Metabolism ,Effluent ,Medical Microbiology ,Fermentation ,Earth Sciences ,Metabolome ,lcsh:Q ,Microbiome ,Pathogens ,Research Article ,Chromatography, Liquid - Abstract
The rumen simulation technique (RUSITEC) is a well-established semicontinuous in vitro model for investigating ruminal fermentation; however, information on the stability of the ruminal bacterial microbiota and metabolome in the RUSITEC system is rarely available. The availability of high resolution methods, such as high-throughput sequencing and metabolomics improve our knowledge about the rumen microbial ecosystem and its fermentation processes. Thus, we used Illumina MiSeq 16S rRNA amplicon sequencing and a combination of direct injection mass spectrometry with a reverse-phase LC-MS/MS to evaluate the dynamics of the bacterial community and the concentration of several metabolites in a RUSITEC experiment as a function of time and in response to a challenge with a pathogenic Clostridium perfringens (C. perfringens) strain. After four days of equilibration, samples were collected on days 5, 6, 7, 10, 12 and 15 of the steady-state and experimental period. From a total of six fermenters, three non-infected fermenters were used for investigating time-dependent alterations; three fermenters were incubated with C. perfringens and compared with the non-infected vessels at days 10, 12 and 15. Along the time-line, there was no statistically significant change of the overall bacterial community, however, some phylotypes were enriched at certain time points. A decrease in Fibrobacter and Elusimicrobia over time was followed by an increase in Firmicutes and Actinobacteria. In contrast, classical fermentation measurements such as pH, redox potential, NH3-N, short chain fatty acids and the concentrations of metabolites determined by metabolomics (biogenic amines, hexoses and amino acids) remained stable throughout the experiment. In response to C. perfringens addition the concentrations of several amino acids increased. Although the overall bacterial community was not altered here either, some minor changes such as an enrichment of Synergistetes and Bacteroidetes were detectable over time. In conclusion, both, the bacterial community composition and the metabolome in the RUSITEC system were relatively stable during the experiment.
- Published
- 2018
8. Very mild disease phenotype of congenic CftrTgH(neoim)Hgu cystic fibrosis mice
- Author
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Leonhard-Marek Sabine, Burmester Marion, Bot Alice, Charizopoulou Nikoletta, Wedekind Dirk, Jansen Silke, Dorsch Martina, Stanke Frauke, Wilke Martina, Tóth Balázs, de Jonge Hugo R, Hedrich Hans-Jürgen, Breves Gerhard, and Tümmler Burkhard
- Subjects
Genetics ,QH426-470 - Abstract
Abstract Background A major boost to cystic fibrosis disease research was given by the generation of various mouse models using gene targeting in embryonal stem cells. Moreover, the introduction of the same mutation on different inbred strains generating congenic strains facilitated the search for modifier genes. From the original CftrTgH(neoim)Hgu mouse model with a divergent genetic background (129/Sv, C57BL/6, HsdOla:MF1) two inbred mutant mouse strains CF/1-CftrTgH(neoim)Hgu and CF/3-CftrTgH(neoim)Hgu had been generated using strict brother × sister mating. CF/1-CftrTgH(neoim)Hgu and CF/3-CftrTgH(neoim)Hgu mice were fertile and showed normal growth and lifespan. In this work the CftrTgH(neoim)Hgu insertional mutation was backcrossed from CF/3-CftrTgH(neoim)Hgu onto the inbred backgrounds C57BL/6J and DBA/2J generating congenic animals in order to clarify the differential impact of the Cftr mutation and the genetic background on the disease phenotype of the cystic fibrosis mutant mice. Clinical and electrophysiological features of the two congenic strains were compared with those of CF/1-CftrTgH(neoim)Hgu and CF/3-CftrTgH(neoim)Hgu and wild type controls. Results Under the standardized housing conditions of the animal facility, the four mouse strains CF/1-CftrTgH(neoim)Hgu, CF/3-CftrTgH(neoim)Hgu, D2.129P2(CF/3)-CftrTgH(neoim)Hgu and B6.129P2(CF/3)-CftrTgH(neoim)Hgu exhibited normal life expectancy. Growth of congenic cystic fibrosis mice was comparable with that of wild type controls. All mice but D2.129P2(CF/3)-CftrTgH(neoim)Hgu females were fertile. Short circuit current measurements revealed characteristic response profiles of the HsdOla:MF1, DBA/2J and C57BL/6J backgrounds in nose, ileum and colon. All cystic fibrosis mouse lines showed the disease-typical hyperresponsiveness to amiloride in the respiratory epithelium. The mean chloride secretory responses to carbachol or forskolin were 15–100% of those of the cognate wild type control animals. Conclusion The amelioration of the clinical features and of the basic defect that had emerged during the generation of CF/3-CftrTgH(neoim)Hgu mice was retained in the congenic mice indicating that the Cftr linkage group or other loci shared between the inbred strains contain(s) the major modifier(s) of attenuation of cystic fibrosis symptoms.
- Published
- 2008
- Full Text
- View/download PDF
9. Contrary seasonal changes of rates of nutrient uptake, organ mass, and voluntary food intake in red deer (Cervus elaphus)
- Author
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Arnold, Walter, primary, Beiglböck, Christoph, additional, Burmester, Marion, additional, Guschlbauer, Maria, additional, Lengauer, Astrid, additional, Schröder, Bernd, additional, Wilkens, Mirja, additional, and Breves, Gerhard, additional
- Published
- 2015
- Full Text
- View/download PDF
10. UNTERSUCHUNG NARRATIVER FÄHIGKEITEN IM WILLIAMS-BEUREN-SYNDROM
- Author
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Krause-Burmester, Marion
- Published
- 2007
11. trans-Resveratrol and ε-viniferin decrease glucose absorption in porcine jejunum and ileum in vitro
- Author
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Guschlbauer, Maria, primary, Klinger, Stefanie, additional, Burmester, Marion, additional, Horn, Jana, additional, Kulling, Sabine E., additional, and Breves, Gerhard, additional
- Published
- 2013
- Full Text
- View/download PDF
12. Mo1752 Murine Norovirus Infection Induces Structural and Functional Intestinal Barrier Changes in the Il10-Deficient Mouse Model of Inflammatory Bowel Disease
- Author
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Keubler, Lydia M., primary, Basic, Marijana, additional, Smoczek, Anna, additional, Jörns, Anne, additional, Burmester, Marion, additional, Schröder, Bernd, additional, Breves, Gerhard, additional, Hedrich, Hans J., additional, and Bleich, Andre, additional
- Published
- 2012
- Full Text
- View/download PDF
13. Very mild disease phenotype of congenic Cftr TgH(neoim)Hgu cystic fibrosis mice
- Author
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Tóth, Balázs, primary, Wilke, Martina, additional, Stanke, Frauke, additional, Dorsch, Martina, additional, Jansen, Silke, additional, Wedekind, Dirk, additional, Charizopoulou, Nikoletta, additional, Bot, Alice, additional, Burmester, Marion, additional, Leonhard-Marek, Sabine, additional, de Jonge, Hugo R, additional, Hedrich, Hans-Jürgen, additional, Breves, Gerhard, additional, and Tümmler, Burkhard, additional
- Published
- 2008
- Full Text
- View/download PDF
14. Very mild disease phenotype of congenic CftrTgH(neoim)Hgu cystic fibrosis mice.
- Author
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Tóth, Balázs, Wilke, Martina, Stanke, Frauke, Dorsch, Martina, Jansen, Silke, Wedekind, Dirk, Charizopoulou, Nikoletta, Bot, Alice, Burmester, Marion, Leonhard-Marek, Sabine, de Jonge, Hugo R., Hedrich, Hans-Jürgen, Breves, Gerhard, and Tümmler, Burkhard
- Subjects
CYSTIC fibrosis ,EMBRYONIC stem cells ,GENETIC mutation ,GENETIC disorders ,GENES ,LABORATORY mice - Abstract
Background: A major boost to cystic fibrosis disease research was given by the generation of various mouse models using gene targeting in embryonal stem cells. Moreover, the introduction of the same mutation on different inbred strains generating congenic strains facilitated the search for modifier genes. From the original Cftr
TgH(neoim)Hgu mouse model with a divergent genetic background (129/Sv, C57BL/6, HsdOla:MF1) two inbred mutant mouse strains CF/1-CftrTgH(neoim)Hgu and CF/3-CftrTgH(neoim)Hgu had been generated using strict brother x sister mating. CF/1-CftrTgH(neoim)Hgu and CF/3-CftrTgH(neoim)Hgu mice were fertile and showed normal growth and lifespan. In this work the CftrTgH(neoim)Hgu insertional mutation was backcrossed from CF/3-CftrTgH(neoim)Hgu onto the inbred backgrounds C57BL/6J and DBA/2J generating congenic animals in order to clarify the differential impact of the Cftr mutation and the genetic background on the disease phenotype of the cystic fibrosis mutant mice. Clinical and electrophysiological features of the two congenic strains were compared with those of CF/1-CftrTgH(neoim)Hgu and CF/3-CftrTgH(neoim)Hgu and wild type controls. Results: Under the standardized housing conditions of the animal facility, the four mouse strains CF/1-CftrTgH(neoim)Hgu , CF/3-CftrTgH(neoim)Hgu , D2.129P2(CF/3)-CftrTgH(neoim)Hgu and B6.129P2(CF/3)-CftrTgH(neoim)Hgu exhibited normal life expectancy. Growth of congenic cystic fibrosis mice was comparable with that of wild type controls. All mice but D2.129P2(CF/3)-CftrTgH(neoim)Hgu females were fertile. Short circuit current measurements revealed characteristic response profiles of the HsdOla:MF1, DBA/2J and C57BL/6J backgrounds in nose, ileum and colon. All cystic fibrosis mouse lines showed the disease-typical hyperresponsiveness to amiloride in the respiratory epithelium. The mean chloride secretory responses to carbachol or forskolin were 15-100% of those of the cognate wild type control animals. Conclusion: The amelioration of the clinical features and of the basic defect that had emerged during the generation of CF/3-CftrTgH(neoim)Hgu mice was retained in the congenic mice indicating that the Cftr linkage group or other loci shared between the inbred strains contain(s) the major modifier(s) of attenuation of cystic fibrosis symptoms. [ABSTRACT FROM AUTHOR]- Published
- 2008
- Full Text
- View/download PDF
15. Die Verrechnung von Steuerforderungen
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Burmester, Marion, primary
- Published
- 1977
- Full Text
- View/download PDF
16. Contrary seasonal changes of rates of nutrient uptake, organ mass, and voluntary food intake in red deer (Cervus elaphus).
- Author
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Arnold W, Beiglböck C, Burmester M, Guschlbauer M, Lengauer A, Schröder B, Wilkens M, and Breves G
- Subjects
- Animals, Appetite physiology, Deer physiology, Eating physiology, Energy Intake physiology, Energy Metabolism physiology, Feeding Behavior, Seasons
- Abstract
Northern ungulates acclimatize to winter conditions with restricted food supply and unfavorable weather conditions by reducing energy expenditure and voluntary food intake. We investigated in a study on red deer whether rates of peptide and glucose transport in the small intestines are also reduced during winter as part of the thrifty phenotype of winter-acclimatized animals, or whether transport rates are increased during winter in order to exploit poor forage more efficiently. Our results support the latter hypothesis. We found in a feeding experiment that total energy intake was considerably lower during winter despite ad libitum feeding. Together with reduced food intake, mass of visceral organs was significantly lower and body fat reserves were used as metabolic fuel in addition to food. However, efficacy of nutrient absorption seemed to be increased simultaneously. Extraction of crude protein from forage was higher in winter animals, at any level of crude protein intake, as indicated by the lower concentration of crude protein in feces. In line with these in vivo results, Ussing chamber experiments revealed greater electrogenic responses to both peptides and glucose in the small intestines of winter-acclimatized animals, and peptide uptake into jejunal brush-border membrane vesicles was increased. We conclude that reduced appetite of red deer during winter avoids energy expenditure for unproductive search of scarcely available food and further renders the energetically costly maintenance of a large gut and visceral organs unnecessary. Nevertheless, extraction of nutrients from forage is more efficient in the winter to attenuate an inevitably negative energy balance., (Copyright © 2015 the American Physiological Society.)
- Published
- 2015
- Full Text
- View/download PDF
17. Very mild disease phenotype of congenic CftrTgH(neoim)Hgu cystic fibrosis mice.
- Author
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Tóth B, Wilke M, Stanke F, Dorsch M, Jansen S, Wedekind D, Charizopoulou N, Bot A, Burmester M, Leonhard-Marek S, de Jonge HR, Hedrich HJ, Breves G, and Tümmler B
- Subjects
- Analysis of Variance, Animals, Body Weight, Carbachol pharmacology, Chlorides metabolism, Colforsin pharmacology, Cystic Fibrosis physiopathology, Female, Fertility, Genetic Linkage, Genotype, Male, Mice, Mice, Congenic, Mice, Inbred CFTR, Microsatellite Repeats, Cystic Fibrosis genetics, Cystic Fibrosis Transmembrane Conductance Regulator genetics, Disease Models, Animal, Phenotype
- Abstract
Background: A major boost to cystic fibrosis disease research was given by the generation of various mouse models using gene targeting in embryonal stem cells. Moreover, the introduction of the same mutation on different inbred strains generating congenic strains facilitated the search for modifier genes. From the original CftrTgH(neoim)Hgu mouse model with a divergent genetic background (129/Sv, C57BL/6, HsdOla:MF1) two inbred mutant mouse strains CF/1-CftrTgH(neoim)Hgu and CF/3-CftrTgH(neoim)Hgu had been generated using strict brother x sister mating. CF/1-CftrTgH(neoim)Hgu and CF/3-CftrTgH(neoim)Hgu mice were fertile and showed normal growth and lifespan. In this work the CftrTgH(neoim)Hgu insertional mutation was backcrossed from CF/3-CftrTgH(neoim)Hgu onto the inbred backgrounds C57BL/6J and DBA/2J generating congenic animals in order to clarify the differential impact of the Cftr mutation and the genetic background on the disease phenotype of the cystic fibrosis mutant mice. Clinical and electrophysiological features of the two congenic strains were compared with those of CF/1-CftrTgH(neoim)Hgu and CF/3-CftrTgH(neoim)Hgu and wild type controls., Results: Under the standardized housing conditions of the animal facility, the four mouse strains CF/1-CftrTgH(neoim)Hgu, CF/3-CftrTgH(neoim)Hgu, D2.129P2(CF/3)-CftrTgH(neoim)Hgu and B6.129P2(CF/3)-CftrTgH(neoim)Hgu exhibited normal life expectancy. Growth of congenic cystic fibrosis mice was comparable with that of wild type controls. All mice but D2.129P2(CF/3)-CftrTgH(neoim)Hgu females were fertile. Short circuit current measurements revealed characteristic response profiles of the HsdOla:MF1, DBA/2J and C57BL/6J backgrounds in nose, ileum and colon. All cystic fibrosis mouse lines showed the disease-typical hyperresponsiveness to amiloride in the respiratory epithelium. The mean chloride secretory responses to carbachol or forskolin were 15-100% of those of the cognate wild type control animals., Conclusion: The amelioration of the clinical features and of the basic defect that had emerged during the generation of CF/3-CftrTgH(neoim)Hgu mice was retained in the congenic mice indicating that the Cftr linkage group or other loci shared between the inbred strains contain(s) the major modifier(s) of attenuation of cystic fibrosis symptoms.
- Published
- 2008
- Full Text
- View/download PDF
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