1,582 results on '"Burk, Robert D"'
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2. Tobacco-Cannabis Co-use and Risk of Substance Use Problems Among Black and Hispanic Adolescent and Young Adult Females in New York City
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Smith, Danielle M., Nucci-Sack, Anne, Shyhalla, Kathleen, Viswanathan, Shankar, Burk, Robert D., Diaz, Angela, and Schlecht, Nicolas F.
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- 2024
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3. Association of the gut microbiome with kidney function and damage in the Hispanic Community Health Study/Study of Latinos (HCHS/SOL)
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Peters, Brandilyn A, Qi, Qibin, Usyk, Mykhaylo, Daviglus, Martha L, Cai, Jianwen, Franceschini, Nora, Lash, James P, Gellman, Marc D, Yu, Bing, Boerwinkle, Eric, Knight, Rob, Burk, Robert D, and Kaplan, Robert C
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Microbiology ,Biological Sciences ,Clinical Research ,Diabetes ,Nutrition ,Kidney Disease ,Renal and urogenital ,Good Health and Well Being ,Humans ,Cross-Sectional Studies ,Gastrointestinal Microbiome ,Hispanic or Latino ,Kidney ,Public Health ,Renal Insufficiency ,Chronic ,Gut microbiome ,chronic kidney disease ,glomerular filtration rate ,metabolites - Abstract
BackgroundThe gut microbiome is altered in chronic kidney disease (CKD), potentially contributing to CKD progression and co-morbidities, but population-based studies of the gut microbiome across a wide range of kidney function and damage are lacking.MethodsIn the Hispanic Community Health Study/Study of Latinos, gut microbiome was assessed by shotgun sequencing of stool (n = 2,438; 292 with suspected CKD). We examined cross-sectional associations of estimated glomerular filtration rate (eGFR), urinary albumin:creatinine (UAC) ratio, and CKD with gut microbiome features. Kidney trait-related microbiome features were interrogated for correlation with serum metabolites (n = 700), and associations of microbiome-related serum metabolites with kidney trait progression were examined in a prospective analysis (n = 3,635).ResultsHigher eGFR was associated with overall gut microbiome composition, greater abundance of species from Prevotella, Faecalibacterium, Roseburia, and Eubacterium, and microbial functions related to synthesis of long-chain fatty acids and carbamoyl-phosphate. Higher UAC ratio and CKD were related to lower gut microbiome diversity and altered overall microbiome composition only in participants without diabetes. Microbiome features related to better kidney health were associated with many serum metabolites (e.g., higher indolepropionate, beta-cryptoxanthin; lower imidazole propionate, deoxycholic acids, p-cresol glucuronide). Imidazole propionate, deoxycholic acid metabolites, and p-cresol glucuronide were associated with prospective reductions in eGFR and/or increases in UAC ratio over ~6 y.ConclusionsKidney function is a significant correlate of the gut microbiome, while the relationship of kidney damage with the gut microbiome depends on diabetes status. Gut microbiome metabolites may contribute to CKD progression.
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- 2023
4. Gut microbiota, blood metabolites, and left ventricular diastolic dysfunction in US Hispanics/Latinos
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Luo, Kai, Taryn, Alkis, Moon, Eun-Hye, Peters, Brandilyn A., Solomon, Scott D., Daviglus, Martha L., Kansal, Mayank M., Thyagarajan, Bharat, Gellman, Marc D., Cai, Jianwen, Burk, Robert D., Knight, Rob, Kaplan, Robert C., Cheng, Susan, Rodriguez, Carlos J., Qi, Qibin, and Yu, Bing
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- 2024
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5. Metabolic and inflammatory perturbation of diabetes associated gut dysbiosis in people living with and without HIV infection
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Luo, Kai, Peters, Brandilyn A., Moon, Jee-Young, Xue, Xiaonan, Wang, Zheng, Usyk, Mykhaylo, Hanna, David B., Landay, Alan L., Schneider, Michael F., Gustafson, Deborah, Weber, Kathleen M., French, Audrey, Sharma, Anjali, Anastos, Kathryn, Wang, Tao, Brown, Todd, Clish, Clary B., Kaplan, Robert C., Knight, Rob, Burk, Robert D., and Qi, Qibin
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- 2024
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6. Variant of the lactase LCT gene explains association between milk intake and incident type 2 diabetes
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Luo, Kai, Chen, Guo-Chong, Zhang, Yanbo, Moon, Jee-Young, Xing, Jiaqian, Peters, Brandilyn A., Usyk, Mykhaylo, Wang, Zheng, Hu, Gang, Li, Jun, Selvin, Elizabeth, Rebholz, Casey M., Wang, Tao, Isasi, Carmen R., Yu, Bing, Knight, Rob, Boerwinkle, Eric, Burk, Robert D., Kaplan, Robert C., and Qi, Qibin
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- 2024
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7. Comprehensive evaluation of shotgun metagenomics, amplicon sequencing, and harmonization of these platforms for epidemiological studies
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Usyk, Mykhaylo, Peters, Brandilyn A, Karthikeyan, Smruthi, McDonald, Daniel, Sollecito, Christopher C, Vazquez-Baeza, Yoshiki, Shaffer, Justin P, Gellman, Marc D, Talavera, Gregory A, Daviglus, Martha L, Thyagarajan, Bharat, Knight, Rob, Qi, Qibin, Kaplan, Robert, and Burk, Robert D
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Microbiology ,Biological Sciences ,Biotechnology ,Genetics ,16S rrna ,HCHS/SOL ,ITS1 ,amplicon ,epidemiology ,fungi ,harmonization ,meta-analysis ,metagenomic ,shotgun - Abstract
In a large cohort of 1,772 participants from the Hispanic Community Health Study/Study of Latinos with overlapping 16SV4 rRNA gene (bacterial amplicon), ITS1 (fungal amplicon), and shotgun sequencing data, we demonstrate that 16SV4 amplicon sequencing and shotgun metagenomics offer the same level of taxonomic accuracy for bacteria at the genus level even at shallow sequencing depths. In contrast, for fungal taxa, we did not observe meaningful agreements between shotgun and ITS1 amplicon results. Finally, we show that amplicon and shotgun data can be harmonized and pooled to yield larger microbiome datasets with excellent agreement (
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- 2023
8. Gut Microbiota, Plasma Metabolomic Profiles, and Carotid Artery Atherosclerosis in HIV Infection
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Wang, Zheng, Peters, Brandilyn A, Usyk, Mykhaylo, Xing, Jiaqian, Hanna, David B, Wang, Tao, Post, Wendy S, Landay, Alan L, Hodis, Howard N, Weber, Kathleen, French, Audrey, Golub, Elizabeth T, Lazar, Jason, Gustafson, Deborah, Kassaye, Seble, Aouizerat, Bradley, Haberlen, Sabina, Malvestutto, Carlos, Budoff, Matthew, Wolinsky, Steven M, Sharma, Anjali, Anastos, Kathryn, Clish, Clary B, Kaplan, Robert C, Burk, Robert D, and Qi, Qibin
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Medical Biochemistry and Metabolomics ,Biomedical and Clinical Sciences ,Atherosclerosis ,HIV/AIDS ,Infectious Diseases ,Cardiovascular ,Clinical Research ,Prevention ,Aetiology ,2.2 Factors relating to the physical environment ,2.1 Biological and endogenous factors ,Infection ,Good Health and Well Being ,Carotid Arteries ,Carotid Artery Diseases ,Carotid Stenosis ,Cross-Sectional Studies ,Female ,Gastrointestinal Microbiome ,HIV Infections ,Humans ,Lysophosphatidylcholines ,Male ,Plaque ,Atherosclerotic ,atherosclerosis ,cardiovascular diseases ,diglycerides ,lipid metabolism ,lipidomics ,Cardiorespiratory Medicine and Haematology ,Clinical Sciences ,Cardiovascular System & Hematology ,Cardiovascular medicine and haematology ,Clinical sciences - Abstract
BackgroundAlterations in gut microbiota and blood metabolomic profiles have been implicated in HIV infection and cardiovascular disease. However, it remains unclear whether alterations in gut microbiota may contribute to disrupted host blood metabolomic profiles in relation to atherosclerosis, especially in the context of HIV infection.MethodsWe analyzed cross-sectional associations between gut microbiota features and carotid artery plaque in 361 women with or at high risk of HIV (67% HIV+), and further integrated plaque-associated microbial features with plasma lipidomic/metabolomic profiles. Furthermore, in 737 women and men, we examined prospective associations of baseline gut bacteria-associated lipidomic and metabolomic profiles with incident carotid artery plaque over 7-year follow-up.ResultsWe found 2 potentially pathogenic bacteria, Fusobacterium and Proteus, were associated with carotid artery plaque; while the beneficial butyrate producer Odoribacter was inversely associated with plaque. Fusobacterium and Proteus were associated with multiple lipids/metabolites which were clustered into 8 modules in network. A module comprised of 9 lysophosphatidylcholines and lysophosphatidylethanolamines and a module comprised of 9 diglycerides were associated with increased risk of carotid artery plaque (risk ratio [95% CI], 1.34 [1.09-1.64] and 1.24 [1.02-1.51] per SD increment, respectively). Functional analyses identified bacterial enzymes in lipid metabolism associated with these plasma lipids. In particular, phospholipase A1 and A2 are the key enzymes in the reactions producing lysophosphatidylcholines and lysophosphatidylethanolamines.ConclusionsAmong individuals with or at high risk of HIV infection, we identified altered gut microbiota and related functional capacities in the lipid metabolism associated with disrupted plasma lipidomic profiles and carotid artery atherosclerosis.
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- 2022
9. Menopause Is Associated with an Altered Gut Microbiome and Estrobolome, with Implications for Adverse Cardiometabolic Risk in the Hispanic Community Health Study/Study of Latinos
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Peters, Brandilyn A, Lin, Juan, Qi, Qibin, Usyk, Mykhaylo, Isasi, Carmen R, Mossavar-Rahmani, Yasmin, Derby, Carol A, Santoro, Nanette, Perreira, Krista M, Daviglus, Martha L, Kominiarek, Michelle A, Cai, Jianwen, Knight, Rob, Burk, Robert D, and Kaplan, Robert C
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Genetics ,Human Genome ,Contraception/Reproduction ,Prevention ,Clinical Research ,Aging ,Estrogen ,Aetiology ,2.1 Biological and endogenous factors ,Good Health and Well Being ,Female ,Male ,Humans ,Gastrointestinal Microbiome ,Public Health ,Menopause ,Gonadal Steroid Hormones ,Hispanic or Latino ,Cardiovascular Diseases ,gut microbiome ,menopause - Abstract
Menopause is a pivotal period during which loss of ovarian hormones increases cardiometabolic risk and may also influence the gut microbiome. However, the menopause-microbiome relationship has not been examined in a large study, and its implications for cardiometabolic disease are unknown. In the Hispanic Community Health Study/Study of Latinos, a population with high burden of cardiometabolic risk factors, shotgun metagenomic sequencing was performed on stool from 2,300 participants (295 premenopausal women, 1,027 postmenopausal women, and 978 men), and serum metabolomics was available on a subset. Postmenopausal women trended toward lower gut microbiome diversity and altered overall composition compared to premenopausal women, while differing less from men, in models adjusted for age and other demographic/behavioral covariates. Differentially abundant taxa for post- versus premenopausal women included Bacteroides sp. strain Ga6A1, Prevotella marshii, and Sutterella wadsworthensis (enriched in postmenopause) and Escherichia coli-Shigella spp., Oscillibacter sp. strain KLE1745, Akkermansia muciniphila, Clostridium lactatifermentans, Parabacteroides johnsonii, and Veillonella seminalis (depleted in postmenopause); these taxa similarly differed between men and women. Postmenopausal women had higher abundance of the microbial sulfate transport system and decreased abundance of microbial β-glucuronidase; these functions correlated with serum progestin metabolites, suggesting involvement of postmenopausal gut microbes in sex hormone retention. In postmenopausal women, menopause-related microbiome alterations were associated with adverse cardiometabolic profiles. In summary, in a large U.S. Hispanic/Latino population, menopause is associated with a gut microbiome more similar to that of men, perhaps related to the common condition of a low estrogen/progesterone state. Future work should examine similarity of results in other racial/ethnic groups. IMPORTANCE The menopausal transition, marked by declining ovarian hormones, is recognized as a pivotal period of cardiometabolic risk. Gut microbiota metabolically interact with sex hormones, but large population studies associating menopause with the gut microbiome are lacking. Our results from a large study of Hispanic/Latino women and men suggest that the postmenopausal gut microbiome in women is slightly more similar to the gut microbiome in men and that menopause depletes specific gut pathogens and decreases the hormone-related metabolic potential of the gut microbiome. At the same time, gut microbes may participate in sex hormone reactivation and retention in postmenopausal women. Menopause-related gut microbiome changes were associated with adverse cardiometabolic risk in postmenopausal women, indicating that the gut microbiome contributes to changes in cardiometabolic health during menopause.
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- 2022
10. Host and gut microbial tryptophan metabolism and type 2 diabetes: an integrative analysis of host genetics, diet, gut microbiome and circulating metabolites in cohort studies
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Qi, Qibin, Li, Jun, Yu, Bing, Moon, Jee-Young, Chai, Jin C, Merino, Jordi, Hu, Jie, Ruiz-Canela, Miguel, Rebholz, Casey, Wang, Zheng, Usyk, Mykhaylo, Chen, Guo-Chong, Porneala, Bianca C, Wang, Wenshuang, Nguyen, Ngoc Quynh, Feofanova, Elena V, Grove, Megan L, Wang, Thomas J, Gerszten, Robert E, Dupuis, Josée, Salas-Salvadó, Jordi, Bao, Wei, Perkins, David L, Daviglus, Martha L, Thyagarajan, Bharat, Cai, Jianwen, Wang, Tao, Manson, JoAnn E, Martínez-González, Miguel A, Selvin, Elizabeth, Rexrode, Kathryn M, Clish, Clary B, Hu, Frank B, Meigs, James B, Knight, Rob, Burk, Robert D, Boerwinkle, Eric, and Kaplan, Robert C
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Obesity ,Human Genome ,Prevention ,Genetics ,Nutrition ,Diabetes ,2.2 Factors relating to the physical environment ,Aetiology ,Metabolic and endocrine ,Oral and gastrointestinal ,Bacteria ,Cohort Studies ,Diabetes Mellitus ,Type 2 ,Diet ,Gastrointestinal Microbiome ,Humans ,Kynurenine ,Lactase ,Tryptophan ,diabetes mellitus ,dietary factors ,genetics ,Clinical Sciences ,Paediatrics and Reproductive Medicine ,Gastroenterology & Hepatology - Abstract
ObjectiveTryptophan can be catabolised to various metabolites through host kynurenine and microbial indole pathways. We aimed to examine relationships of host and microbial tryptophan metabolites with incident type 2 diabetes (T2D), host genetics, diet and gut microbiota.MethodWe analysed associations between circulating levels of 11 tryptophan metabolites and incident T2D in 9180 participants of diverse racial/ethnic backgrounds from five cohorts. We examined host genome-wide variants, dietary intake and gut microbiome associated with these metabolites.ResultsTryptophan, four kynurenine-pathway metabolites (kynurenine, kynurenate, xanthurenate and quinolinate) and indolelactate were positively associated with T2D risk, while indolepropionate was inversely associated with T2D risk. We identified multiple host genetic variants, dietary factors, gut bacteria and their potential interplay associated with these T2D-relaetd metabolites. Intakes of fibre-rich foods, but not protein/tryptophan-rich foods, were the dietary factors most strongly associated with tryptophan metabolites. The fibre-indolepropionate association was partially explained by indolepropionate-associated gut bacteria, mostly fibre-using Firmicutes. We identified a novel association between a host functional LCT variant (determining lactase persistence) and serum indolepropionate, which might be related to a host gene-diet interaction on gut Bifidobacterium, a probiotic bacterium significantly associated with indolepropionate independent of other fibre-related bacteria. Higher milk intake was associated with higher levels of gut Bifidobacterium and serum indolepropionate only among genetically lactase non-persistent individuals.ConclusionHigher milk intake among lactase non-persistent individuals, and higher fibre intake were associated with a favourable profile of circulating tryptophan metabolites for T2D, potentially through the host-microbial cross-talk shifting tryptophan metabolism toward gut microbial indolepropionate production.
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- 2022
11. Gut microbiota, circulating inflammatory markers and metabolites, and carotid artery atherosclerosis in HIV infection
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Wang, Zheng, Peters, Brandilyn A., Bryant, MacKenzie, Hanna, David B., Schwartz, Tara, Wang, Tao, Sollecito, Christopher C., Usyk, Mykhaylo, Grassi, Evan, Wiek, Fanua, Peter, Lauren St., Post, Wendy S., Landay, Alan L., Hodis, Howard N., Weber, Kathleen M., French, Audrey, Golub, Elizabeth T., Lazar, Jason, Gustafson, Deborah, Sharma, Anjali, Anastos, Kathryn, Clish, Clary B., Burk, Robert D., Kaplan, Robert C., Knight, Rob, and Qi, Qibin
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- 2023
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12. Kernel-based genetic association analysis for microbiome phenotypes identifies host genetic drivers of beta-diversity
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Liu, Hongjiao, Ling, Wodan, Hua, Xing, Moon, Jee-Young, Williams-Nguyen, Jessica S., Zhan, Xiang, Plantinga, Anna M., Zhao, Ni, Zhang, Angela, Knight, Rob, Qi, Qibin, Burk, Robert D., Kaplan, Robert C., and Wu, Michael C.
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- 2023
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13. Psychosocial Effects of Frequent Cannabis Smoking in Adolescent Women of Color: Results from a Prospective Cohort of Inner-City Youth
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Duroseau, Nathalie, Niu, Li, Wilson, Karen, Nucci-Sack, Anne, Burk, Robert D., Diaz, Angela, and Schlecht, Nicolas F.
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- 2023
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14. The Gut Microbiome, Microbial Metabolites, and Cardiovascular Disease in People Living with HIV
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Peters, Brandilyn A., Burk, Robert D., Kaplan, Robert C., and Qi, Qibin
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- 2023
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15. Microbial co-occurrence complicates associations of gut microbiome with US immigration, dietary intake and obesity
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Wang, Zheng, Usyk, Mykhaylo, Vázquez-Baeza, Yoshiki, Chen, Guo-Chong, Isasi, Carmen R, Williams-Nguyen, Jessica S, Hua, Simin, McDonald, Daniel, Thyagarajan, Bharat, Daviglus, Martha L, Cai, Jianwen, North, Kari E, Wang, Tao, Knight, Rob, Burk, Robert D, Kaplan, Robert C, and Qi, Qibin
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Nutrition ,Obesity ,Cardiovascular ,Oral and gastrointestinal ,Acculturation ,Adult ,Aged ,Aged ,80 and over ,Bacteria ,Cohort Studies ,Diet ,Eating ,Emigrants and Immigrants ,Emigration and Immigration ,Feces ,Female ,Gastrointestinal Microbiome ,Hispanic or Latino ,Humans ,Male ,Metagenomics ,Middle Aged ,RNA ,Ribosomal ,16S ,United States ,Microbiome ,Hispanic population ,Environmental Sciences ,Biological Sciences ,Information and Computing Sciences ,Bioinformatics - Abstract
BackgroundObesity and related comorbidities are major health concerns among many US immigrant populations. Emerging evidence suggests a potential involvement of the gut microbiome. Here, we evaluated gut microbiome features and their associations with immigration, dietary intake, and obesity in 2640 individuals from a population-based study of US Hispanics/Latinos.ResultsThe fecal shotgun metagenomics data indicate that greater US exposure is associated with reduced ɑ-diversity, reduced functions of fiber degradation, and alterations in individual taxa, potentially related to a westernized diet. However, a majority of gut bacterial genera show paradoxical associations, being reduced with US exposure and increased with fiber intake, but increased with obesity. The observed paradoxical associations are not explained by host characteristics or variation in bacterial species but might be related to potential microbial co-occurrence, as seen by positive correlations among Roseburia, Prevotella, Dorea, and Coprococcus. In the conditional analysis with mutual adjustment, including all genera associated with both obesity and US exposure in the same model, the positive associations of Roseburia and Prevotella with obesity did not persist, suggesting that their positive associations with obesity might be due to their co-occurrence and correlations with obesity-related taxa, such as Dorea and Coprococcus.ConclusionsAmong US Hispanics/Latinos, US exposure is associated with unfavorable gut microbiome profiles for obesity risk, potentially related to westernized diet during acculturation. Microbial co-occurrence could be an important factor to consider in future studies relating individual gut microbiome taxa to environmental factors and host health and disease.
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- 2021
16. Tryptophan metabolism, gut microbiota, and carotid artery plaque in women with and without HIV infection
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Luo, Kai, Wang, Zheng, Peters, Brandilyn A., Hanna, David B., Wang, Tao, Sollecito, Christopher C., Grassi, Evan, Wiek, Fanua, St. Peter, Lauren, Usyk, Mykhaylo, Post, Wendy S., Landay, Alan L., Hodis, Howard N., Weber, Kathleen M., French, Audrey, Topper, Elizabeth F., Lazar, Jason, Gustafson, Deborah, Sharma, Anjali, Anastos, Kathryn, Clish, Clary B., Knight, Rob, Kaplan, Robert C., Burk, Robert D., and Qi, Qibin
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- 2024
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17. Dietary factors, gut microbiota, and serum trimethylamine-N-oxide associated with cardiovascular disease in the Hispanic Community Health Study/Study of Latinos
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Mei, Zhendong, Chen, Guo-Chong, Wang, Zheng, Usyk, Mykhaylo, Yu, Bing, Baeza, Yoshiki Vazquez, Humphrey, Greg, Benitez, Rodolfo Salido, Li, Jun, Williams-Nguyen, Jessica S, Daviglus, Martha L, Hou, Lifang, Cai, Jianwen, Zheng, Yan, Knight, Rob, Burk, Robert D, Boerwinkle, Eric, Kaplan, Robert C, and Qi, Qibin
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Biomedical and Clinical Sciences ,Nutrition and Dietetics ,Clinical Research ,Cardiovascular ,Prevention ,Nutrition ,Good Health and Well Being ,Adult ,Aged ,Biomarkers ,Cardiovascular Diseases ,Cross-Sectional Studies ,Diet ,Female ,Gastrointestinal Microbiome ,Hispanic or Latino ,Humans ,Male ,Methylamines ,Middle Aged ,Public Health ,cardiovascular disease ,diet ,gut microbiota ,trimethylamine-N-oxide ,Hispanic Americans ,Engineering ,Medical and Health Sciences ,Nutrition & Dietetics ,Clinical sciences ,Nutrition and dietetics - Abstract
BackgroundTrimethylamine-N-oxide (TMAO), a diet-derived and gut microbiota-related metabolite, is associated with cardiovascular disease (CVD). However, major dietary determinants and specific gut bacterial taxa related to TMAO remain to be identified in humans.ObjectivesWe aimed to identify dietary and gut microbial factors associated with circulating TMAO.MethodsThis cross-sectional study included 3972 participants (57.3% women) aged 18-74 y from the Hispanic Community Health Study/Study of Latinos in the United States. Dietary information was collected by 24-h dietary recalls at baseline interview (2008-2011), and baseline serum TMAO and its precursors were measured by an untargeted approach. Gut microbiome was profiled by shotgun metagenomic sequencing in a subset of participants (n = 626) during a follow-up visit (2016-2018). Logistic and linear regression were used to examine associations of inverse-normalized metabolites with prevalent CVD, dietary intake, and bacterial species, respectively, after adjustment for sociodemographic, behavioral, and clinical factors.ResultsTMAO was positively associated with prevalent CVD (case number = 279; OR = 1.34; 95% CI: 1.17, 1.54, per 1-SD). Fish (P = 1.26 × 10-17), red meat (P = 3.33 × 10-16), and egg (P = 3.89 × 10-5) intakes were top dietary factors positively associated with TMAO. We identified 9 gut bacterial species significantly associated with TMAO (false discovery rate
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- 2021
18. Primary HPV and Molecular Cervical Cancer Screening in US Women Living With Human Immunodeficiency Virus
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Strickler, Howard D, Keller, Marla J, Hessol, Nancy A, Eltoum, Isam-Eldin, Einstein, Mark H, Castle, Philip E, Massad, L Stewart, Flowers, Lisa, Rahangdale, Lisa, Atrio, Jessica M, Ramirez, Catalina, Minkoff, Howard, Adimora, Adaora A, Ofotokun, Igho, Colie, Christine, Huchko, Megan J, Fischl, Margaret, Wright, Rodney, D’Souza, Gypsyamber, Leider, Jason, Diaz, Olga, Sanchez-Keeland, Lorraine, Shrestha, Sadeep, Xie, Xianhong, Xue, Xiaonan, Anastos, Kathryn, Palefsky, Joel M, and Burk, Robert D
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Reproductive Medicine ,Biomedical and Clinical Sciences ,Clinical Sciences ,Sexually Transmitted Infections ,HIV/AIDS ,Clinical Research ,Infectious Diseases ,Cervical Cancer ,Cancer ,Alphapapillomavirus ,Early Detection of Cancer ,Female ,HIV ,HIV Infections ,Human papillomavirus 16 ,Human papillomavirus 18 ,Humans ,Mass Screening ,Middle Aged ,Papillomaviridae ,Papillomavirus Infections ,Pregnancy ,Uterine Cervical Neoplasms ,Vaginal Smears ,Uterine Cervical Dysplasia ,human papillomavirus ,cervical cancer screening ,p16/Ki-67 ,primary HPV screening ,Biological Sciences ,Medical and Health Sciences ,Microbiology ,Clinical sciences - Abstract
BackgroundPrimary human papillomavirus (HPV) screening (PHS) utilizes oncogenic human papillomavirus (oncHPV) testing as the initial cervical cancer screening method and typically, if positive, additional reflex-triage (eg, HPV16/18-genotyping, Pap testing). While US guidelines support PHS usage in the general population, PHS has been little studied in women living with HIV (WLWH).MethodsWe enrolled n = 865 WLWH (323 from the Women's Interagency HIV Study [WIHS] and 542 from WIHS-affiliated colposcopy clinics). All participants underwent Pap and oncHPV testing, including HPV16/18-genotyping. WIHS WLWH who tested oncHPV[+] or had cytologic atypical squamous cells of undetermined significance or worse (ASC-US+) underwent colposcopy, as did a random 21% of WLWH who were oncHPV[-]/Pap[-] (controls). Most participants additionally underwent p16/Ki-67 immunocytochemistry.ResultsMean age was 46 years, median CD4 was 592 cells/µL, 95% used antiretroviral therapy. Seventy WLWH had histologically-determined cervical intraepithelial neoplasia grade 2 or greater (CIN-2+), of which 33 were defined as precancer (ie, [i] CIN-3+ or [ii] CIN-2 if concurrent with cytologic high grade squamous intraepithelial lesions [HSILs]). PHS had 87% sensitivity (Se) for precancer, 9% positive predictive value (PPV), and a 35% colposcopy referral rate (Colpo). "PHS with reflex HPV16/18-genotyping and Pap testing" had 84% Se, 16% PPV, 30% Colpo. PHS with only HPV16/18-genotyping had 24% Colpo. "Concurrent oncHPV and Pap Testing" (Co-Testing) had 91% Se, 12% PPV, 40% Colpo. p16/Ki-67 immunochemistry had the highest PPV, 20%, but 13% specimen inadequacy.ConclusionsPHS with reflex HPV16/18-genotyping had fewer unnecessary colposcopies and (if confirmed) could be a potential alternative to Co-Testing in WLWH.
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- 2021
19. Anaerobic pathogens associated with OSA may contribute to pathophysiology via amino-acid depletion
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Elgart, Michael, Zhang, Ying, Zhang, Yuan, Yu, Bing, Kim, Youngmee, Zee, Phyllis C., Gellman, Marc D., Boerwinkle, Eric, Daviglus, Martha L., Cai, Jianwen, Redline, Susan, Burk, Robert D., Kaplan, Robert, and Sofer, Tamar
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- 2023
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20. Large-scale association analyses identify host factors influencing human gut microbiome composition
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Kurilshikov, Alexander, Medina-Gomez, Carolina, Bacigalupe, Rodrigo, Radjabzadeh, Djawad, Wang, Jun, Demirkan, Ayse, Le Roy, Caroline I, Raygoza Garay, Juan Antonio, Finnicum, Casey T, Liu, Xingrong, Zhernakova, Daria V, Bonder, Marc Jan, Hansen, Tue H, Frost, Fabian, Rühlemann, Malte C, Turpin, Williams, Moon, Jee-Young, Kim, Han-Na, Lüll, Kreete, Barkan, Elad, Shah, Shiraz A, Fornage, Myriam, Szopinska-Tokov, Joanna, Wallen, Zachary D, Borisevich, Dmitrii, Agreus, Lars, Andreasson, Anna, Bang, Corinna, Bedrani, Larbi, Bell, Jordana T, Bisgaard, Hans, Boehnke, Michael, Boomsma, Dorret I, Burk, Robert D, Claringbould, Annique, Croitoru, Kenneth, Davies, Gareth E, van Duijn, Cornelia M, Duijts, Liesbeth, Falony, Gwen, Fu, Jingyuan, van der Graaf, Adriaan, Hansen, Torben, Homuth, Georg, Hughes, David A, Ijzerman, Richard G, Jackson, Matthew A, Jaddoe, Vincent WV, Joossens, Marie, Jørgensen, Torben, Keszthelyi, Daniel, Knight, Rob, Laakso, Markku, Laudes, Matthias, Launer, Lenore J, Lieb, Wolfgang, Lusis, Aldons J, Masclee, Ad AM, Moll, Henriette A, Mujagic, Zlatan, Qibin, Qi, Rothschild, Daphna, Shin, Hocheol, Sørensen, Søren J, Steves, Claire J, Thorsen, Jonathan, Timpson, Nicholas J, Tito, Raul Y, Vieira-Silva, Sara, Völker, Uwe, Völzke, Henry, Võsa, Urmo, Wade, Kaitlin H, Walter, Susanna, Watanabe, Kyoko, Weiss, Stefan, Weiss, Frank U, Weissbrod, Omer, Westra, Harm-Jan, Willemsen, Gonneke, Payami, Haydeh, Jonkers, Daisy MAE, Arias Vasquez, Alejandro, de Geus, Eco JC, Meyer, Katie A, Stokholm, Jakob, Segal, Eran, Org, Elin, Wijmenga, Cisca, Kim, Hyung-Lae, Kaplan, Robert C, Spector, Tim D, Uitterlinden, Andre G, Rivadeneira, Fernando, Franke, Andre, Lerch, Markus M, Franke, Lude, Sanna, Serena, D’Amato, Mauro, and Pedersen, Oluf
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Microbiology ,Biological Sciences ,Genetics ,Human Genome ,Biotechnology ,Clinical Research ,Digestive Diseases ,Nutrition ,2.1 Biological and endogenous factors ,Aetiology ,Oral and gastrointestinal ,Adolescent ,Adult ,Bifidobacterium ,Child ,Child ,Preschool ,Cohort Studies ,Female ,Gastrointestinal Microbiome ,Genetic Variation ,Genome-Wide Association Study ,Humans ,Lactase ,Linkage Disequilibrium ,Male ,Mendelian Randomization Analysis ,Metabolism ,Quantitative Trait Loci ,RNA ,Ribosomal ,16S ,Medical and Health Sciences ,Developmental Biology ,Agricultural biotechnology ,Bioinformatics and computational biology - Abstract
To study the effect of host genetics on gut microbiome composition, the MiBioGen consortium curated and analyzed genome-wide genotypes and 16S fecal microbiome data from 18,340 individuals (24 cohorts). Microbial composition showed high variability across cohorts: only 9 of 410 genera were detected in more than 95% of samples. A genome-wide association study of host genetic variation regarding microbial taxa identified 31 loci affecting the microbiome at a genome-wide significant (P
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- 2021
21. Long-acting reversible contraception and condom use: A cohort study of female adolescents and young adults in New York City
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Loh, Miranda, Niu, Li, Arden, Martha, Burk, Robert D., Diaz, Angela, and Schlecht, Nicolas F.
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- 2023
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22. The combined finding of HPV 16, 18, or 45 and cytologic Atypical Glandular Cells (AGC) indicates a greatly elevated risk of in situ and invasive cervical adenocarcinoma
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Schiffman, Mark, Mirabello, Lisa, Egemen, Didem, Befano, Brian, Xiao, Yanzi, Wentzensen, Nicolas, Raine-Bennett, Tina, Nayar, Ritu, Cheung, Li C., Rositch, Anne, Beaty, Terri, Perkins, Rebecca B., de Sanjose, Silvia, Lorey, Thomas, Castle, Philip E., and Burk, Robert D.
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- 2023
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23. Altered Gut Microbiota and Host Metabolite Profiles in Women With Human Immunodeficiency Virus
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Wang, Zheng, Usyk, Mykhaylo, Sollecito, Christopher C, Qiu, Yunping, Williams-Nguyen, Jessica, Hua, Simin, Gradissimo, Ana, Wang, Tao, Xue, Xiaonan, Kurland, Irwin J, Ley, Klaus, Landay, Alan L, Anastos, Kathryn, Knight, Rob, Kaplan, Robert C, Burk, Robert D, and Qi, Qibin
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Medical Biochemistry and Metabolomics ,Medical Microbiology ,Biomedical and Clinical Sciences ,Infectious Diseases ,HIV/AIDS ,2.1 Biological and endogenous factors ,Aetiology ,Infection ,Female ,Gastrointestinal Microbiome ,HIV ,HIV Infections ,Humans ,Metabolomics ,RNA ,Ribosomal ,16S ,HIV infection ,gut microbiota ,metabolomics ,integrative analysis ,Biological Sciences ,Medical and Health Sciences ,Microbiology ,Clinical sciences - Abstract
BackgroundAlterations in gut microbiota (GMB) and host metabolites have been noted in individuals with HIV. However, it remains unclear whether alterations in GMB and related functional groups contribute to disrupted host metabolite profiles in these individuals.MethodsThis study included 185 women (128 with longstanding HIV infection, 88% under antiretroviral therapy; and 57 women without HIV from the same geographic location with comparable characteristics). Stool samples were analyzed by 16S rRNA V4 region sequencing, and GMB function was inferred by PICRUSt. Plasma metabolomic profiling was performed using liquid chromatography-tandem mass spectrometry, and 133 metabolites (amino acids, biogenic amines, acylcarnitines, and lipids) were analyzed.ResultsFour predominant bacterial genera were identified as associated with HIV infection, with higher abundances of Ruminococcus and Oscillospira and lower abundances of Bifidobacterium and Collinsella in women with HIV than in those without. Women with HIV showed a distinct plasma metabolite profile, which featured elevated glycerophospholipid levels compared with those without HIV. Functional analyses also indicated that GMB lipid metabolism was enriched in women with HIV. Ruminococcus and Oscillospira were among the top bacterial genera contributing to the GMB glycerophospholipid metabolism pathway and showed positive correlations with host plasma glycerophospholipid levels. One bacterial functional capacity in the acetate and propionate biosynthesis pathway was identified to be mainly contributed by Bifidobacterium; this functional capacity was lower in women with HIV than in women without HIV.ConclusionsOur integrative analyses identified altered GMB with related functional capacities that might be associated with disrupted plasma metabolite profiles in women with HIV.
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- 2020
24. Author Correction: Gut microbiome composition in the Hispanic Community Health Study/Study of Latinos is shaped by geographic relocation, environmental factors, and obesity
- Author
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Kaplan, Robert C, Wang, Zheng, Usyk, Mykhaylo, Sotres-Alvarez, Daniela, Daviglus, Martha L, Schneiderman, Neil, Talavera, Gregory A, Gellman, Marc D, Thyagarajan, Bharat, Moon, Jee-Young, Vázquez-Baeza, Yoshiki, McDonald, Daniel, Williams-Nguyen, Jessica S, Wu, Michael C, North, Kari E, Shaffer, Justin, Sollecito, Christopher C, Qi, Qibin, Isasi, Carmen R, Wang, Tao, Knight, Rob, and Burk, Robert D
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Microbiology ,Biological Sciences ,Obesity ,Nutrition ,Environmental Sciences ,Information and Computing Sciences ,Bioinformatics - Abstract
Following publication of the original paper [1], an error was reported in the third paragraph in the section "Analysis of GMB composition and its correlates" (page 3 of the PDF). The first sentence of the text should refer to Table 2, but mistakenly refers to Table 1.
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- 2020
25. Epidemiological evidence that common HPV types may be common because of their ability to evade immune surveillance: Results from the Women's Interagency HIV study
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Castle, Philip E, Burk, Robert D, Massad, Leslie S, Eltoum, Isam‐Eldin, Hall, Charles B, Hessol, Nancy A, Anastos, Kathryn, Xie, Xianhong, Minkoff, Howard, Xue, Xiaonan, D'Souza, Gypsyamber, Flowers, Lisa, Colie, Christine, Rahangdale, Lisa, Fischl, Margaret A, Palefsky, Joel M, and Strickler, Howard D
- Subjects
Medical Microbiology ,Biomedical and Clinical Sciences ,Immunology ,Infectious Diseases ,HIV/AIDS ,Sexually Transmitted Infections ,Infection ,Good Health and Well Being ,Adult ,CD4 Lymphocyte Count ,Cervix Uteri ,DNA ,Viral ,Female ,HIV Seropositivity ,Humans ,Immune Evasion ,Papanicolaou Test ,Papillomaviridae ,Papillomavirus Infections ,Phylogeny ,Prevalence ,Prospective Studies ,Uterine Cervical Neoplasms ,Young Adult ,Oncology and Carcinogenesis ,Oncology & Carcinogenesis ,Oncology and carcinogenesis - Abstract
Infection by human papillomavirus (HPV) type 16, the most oncogenic HPV type, was found to be the least affected by HIV-status and CD4 count of any of the approximately 13 oncogenic HPV types. This relative independence from host immune status has been interpreted as evidence that HPV16 may have an innate ability to avoid the effects of immunosurveillance. However, the impact of immune status on other individual HPV types has not been carefully assessed. We studied type-specific HPV infection in a cohort of 2,470 HIV-positive (HIV[+]) and 895 HIV-negative (HIV[-]) women. Semi-annually collected cervicovaginal lavages were tested for >40 HPV types. HPV type-specific prevalence ratios (PRs), incidence and clearance hazard ratios (HRs), were calculated by contrasting HPV types detected in HIV[+] women with CD4
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- 2020
26. Gut microbiome composition in the Hispanic Community Health Study/Study of Latinos is shaped by geographic relocation, environmental factors, and obesity
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Kaplan, Robert C, Wang, Zheng, Usyk, Mykhaylo, Sotres-Alvarez, Daniela, Daviglus, Martha L, Schneiderman, Neil, Talavera, Gregory A, Gellman, Marc D, Thyagarajan, Bharat, Moon, Jee-Young, Vázquez-Baeza, Yoshiki, McDonald, Daniel, Williams-Nguyen, Jessica S, Wu, Michael C, North, Kari E, Shaffer, Justin, Sollecito, Christopher C, Qi, Qibin, Isasi, Carmen R, Wang, Tao, Knight, Rob, and Burk, Robert D
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Microbiology ,Biological Sciences ,Obesity ,Nutrition ,Oral and gastrointestinal ,Good Health and Well Being ,Acculturation ,Adult ,Aged ,Cohort Studies ,Diet ,Emigration and Immigration ,Female ,Gastrointestinal Microbiome ,Hispanic or Latino ,Humans ,Latin America ,Male ,Middle Aged ,Microbiome ,Epidemiology ,Hispanic population ,Mycobiome ,Environmental Sciences ,Information and Computing Sciences ,Bioinformatics - Abstract
BackgroundHispanics living in the USA may have unrecognized potential birthplace and lifestyle influences on the gut microbiome. We report a cross-sectional analysis of 1674 participants from four centers of the Hispanic Community Health Study/Study of Latinos (HCHS/SOL), aged 18 to 74 years old at recruitment.ResultsAmplicon sequencing of 16S rRNA gene V4 and fungal ITS1 fragments from self-collected stool samples indicate that the host microbiome is determined by sociodemographic and migration-related variables. Those who relocate from Latin America to the USA at an early age have reductions in Prevotella to Bacteroides ratios that persist across the life course. Shannon index of alpha diversity in fungi and bacteria is low in those who relocate to the USA in early life. In contrast, those who relocate to the USA during adulthood, over 45 years old, have high bacterial and fungal diversity and high Prevotella to Bacteroides ratios, compared to USA-born and childhood arrivals. Low bacterial diversity is associated in turn with obesity. Contrasting with prior studies, our study of the Latino population shows increasing Prevotella to Bacteroides ratio with greater obesity. Taxa within Acidaminococcus, Megasphaera, Ruminococcaceae, Coriobacteriaceae, Clostridiales, Christensenellaceae, YS2 (Cyanobacteria), and Victivallaceae are significantly associated with both obesity and earlier exposure to the USA, while Oscillospira and Anaerotruncus show paradoxical associations with both obesity and late-life introduction to the USA.ConclusionsOur analysis of the gut microbiome of Latinos demonstrates unique features that might be responsible for health disparities affecting Hispanics living in the USA.
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- 2019
27. Healthful eating patterns, serum metabolite profile and risk of diabetes in a population-based prospective study of US Hispanics/Latinos
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Chen, Guo-Chong, Chai, Jin Choul, Xing, Jiaqian, Moon, Jee-Young, Shan, Zhilei, Yu, Bing, Mossavar-Rahman, Yasmin, Sotres-Alvarez, Daniela, Li, Jun, Mattei, Josiemer, Daviglus, Martha L., Perkins, David L., Burk, Robert D., Boerwinkle, Eric, Kaplan, Robert C., Hu, Frank B., and Qi, Qibin
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- 2022
- Full Text
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28. Tryptophan metabolism, gut microbiota, and carotid artery plaque in women with and without HIV infection
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Luo, Kai, Wang, Zheng, Peters, Brandilyn A., Hanna, David B., Wang, Tao, Sollecito, Christopher C., Grassi, Evan, Wiek, Fanua, Peter, Lauren St, Usyk, Mykhaylo, Post, Wendy S., Landay, Alan L., Hodis, Howard N., Weber, Kathleen M., French, Audrey, Golub, Elizabeth T., Lazar, Jason, Gustafson, Deborah, Sharma, Anjali, Anastos, Kathryn, Clish, Clary B., Knight, Rob, Kaplan, Robert C., Burk, Robert D., and Qi, Qibin
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- 2023
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29. Characterization of oral microbiota in HPV and non-HPV head and neck squamous cell carcinoma and its association with patient outcomes
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Chan, Jason Y.K., Cheung, Man Kit, Lan, Linlin, Ng, Cherrie, Lau, Eric H.L., Yeung, Zenon W.C., Wong, Eddy W.Y., Leung, Leanne, Qu, Xinyu, Cai, Liuyang, Zhu, Hengyan, Boon, Siaw Shi, Burk, Robert D., Chan, Paul K.S., and Chen, Zigui
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- 2022
- Full Text
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30. Methylation of High-Risk Human Papillomavirus Genomes are Associated with Cervical Precancer in HIV-positive Women
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Gradissimo, Ana, Lam, Jessica, Attonito, John D, Palefsky, Joel, Massad, L Stewart, Xie, Xianhong, Eltoum, Isam-Eldin, Rahangdale, Lisa, Fischl, Margaret A, Anastos, Kathryn, Minkoff, Howard, Xue, Xiaonan, D'Souza, Gypsyamber, Flowers, Lisa C, Colie, Christine, Shrestha, Sadeep, Hessol, Nancy A, Strickler, Howard D, and Burk, Robert D
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Reproductive Medicine ,Biomedical and Clinical Sciences ,HIV/AIDS ,Human Genome ,Infectious Diseases ,Clinical Research ,Cancer ,Women's Health ,Sexually Transmitted Infections ,Genetics ,Cervical Cancer ,Clinical Trials and Supportive Activities ,Infection ,Adult ,Case-Control Studies ,Female ,Genomics ,HIV Infections ,Humans ,Methylation ,Papillomavirus Infections ,Risk Factors ,Medical and Health Sciences ,Epidemiology ,Biomedical and clinical sciences ,Health sciences - Abstract
BackgroundHIV-positive women are at substantial risk of HPV-associated cervical neoplasia caused by high-risk (HR) HPVs. Methylation of the HPV genome is associated with cervical intraepithelial neoplasia grade 3 (CIN3) in HIV-negative women, yet it is unknown whether this holds true for HIV-positive women.MethodsWe designed a case-control study within the Women's Interagency HIV Study (WIHS) cohort comparing HIV-positive CIN3 cases (N = 72) to HIV-positive controls without detectable CIN2+. The unit of analysis and matching was HPV-type infection. Cases with ≥2 HR-HPV types (N = 23; 32%) had a separate control for each HR-HPV type. We developed and utilized next-generation sequencing (NGS) methylation assays for 12 different HR-HPVs, focusing on CpG sites in the L1/L2 regions.ResultsSignificant case-control differences in individual CpG site methylation levels were observed for multiple alpha-9 (HPV16/31/35/58) and alpha-7 HPV (HPV18/39/45) types, based on dichotomization of tertile levels (T3 vs. T1 and T2). Analyses combining homologous CpG sites [e.g., HPV16-L1-5608/HPV31-L1-5521/HPV35-L2L1-5570; OR = 7.28; 95% confidence interval (CI): 2.75-19.3], and (e.g., HPV18-L1-7062/HPV45-L1-7066; OR = 6.94; 95% CI: 1.23-39.3) were significant in separate case-control comparisons. In cases with multiple HR-HPVs, we tested and confirmed the hypothesis that one HR-HPV type would have higher methylation than other types detected, consistent with there being a single HR-HPV causally related to a lesion.ConclusionsCIN3 is associated with elevated L1/L2 CpG methylation levels in HIV-positive women.ImpactHPV DNA CpG methylation is a promising triage option in HIV-positive women testing positive for HR-HPV types and provides risk attribution in women with multiple HPV type infections.
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- 2018
31. Racial differences in human papilloma virus types amongst United States women with HIV and cervical precancer
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Keller, Marla J, Burk, Robert D, Massad, L Stewart, Eltoum, Isam-Eldin, Hessol, Nancy A, Anastos, Kathryn, Xie, Xianhong, Minkoff, Howard, Xue, Xiaonan, Reimers, Laura L, Kuniholm, Mark, DʼSouza, Gypsyamber, Colie, Christine, Aouizerat, Bradley, Palefsky, Joel M, and Strickler, Howard D
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Reproductive Medicine ,Biomedical and Clinical Sciences ,Public Health ,Health Sciences ,HIV/AIDS ,Infectious Diseases ,Clinical Research ,Sexually Transmitted Infections ,Cancer ,Cervical Cancer ,Immunization ,Prevention ,Vaccine Related ,Infection ,Good Health and Well Being ,Adult ,Black or African American ,Cervix Uteri ,Female ,Genotype ,Genotyping Techniques ,HIV Infections ,Humans ,Longitudinal Studies ,Middle Aged ,Papanicolaou Test ,Papillomaviridae ,Papillomavirus Infections ,Polymerase Chain Reaction ,Precancerous Conditions ,Prevalence ,United States ,White People ,cervical precancer ,ethnicity ,HIV ,human papillomavirus ,race ,Biological Sciences ,Medical and Health Sciences ,Psychology and Cognitive Sciences ,Virology ,Biomedical and clinical sciences ,Health sciences - Abstract
ObjectiveRecent studies reported a lower human papillomavirus 16 (HPV16) prevalence in cervical precancer among African American than Caucasian women in the general population. We assessed this relationship in women with HIV.DesignWomen living with or at risk for HIV in the Women's Interagency HIV Study were followed semi-annually with Pap tests, colposcopy/histology (if indicated), and collection of cervicovaginal lavage samples for HPV testing by PCR. Racial and ethnic groups were defined using genomic Ancestry Informative Markers (AIMs).ResultsAmong 175 cases of cervical intraepithelial neoplasia 3 or worse (CIN-3+), 154 were diagnosed in women with HIV. African American (27%) and Hispanic (37%) cases were significantly less likely than Caucasian (62%) women to test positive for HPV16 (P = 0.01). In multivariate logistic regression models, these associations remained significant for African Americans (odds ratio = 0.13; 95% confidence interval (CI) 0.04-0.44; P = 0.001) but not Hispanics, after controlling for HIV status, CD4 count, history of AIDS, age, smoking, and sexual behavior. Limiting the analysis to women with HIV did not change the findings.ConclusionHPV16 prevalence is lower in African American compared with Caucasian women with HIV and cervical precancer, independent of immune status. Future studies to determine why these racial differences exist are warranted, and whether there are similar associations between race and invasive cervical cancer in women with HIV. Further, HPV types not covered by quadrivalent and bivalent vaccines may play an especially important role in cervical precancer among HIV-positive African American women, a possible advantage to using nonavalent HPV vaccine in this population.
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- 2018
32. molBV reveals immune landscape of bacterial vaginosis and predicts human papillomavirus infection natural history
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Usyk, Mykhaylo, Schlecht, Nicolas F., Pickering, Sarah, Williams, LaShanda, Sollecito, Christopher C., Gradissimo, Ana, Porras, Carolina, Safaeian, Mahboobeh, Pinto, Ligia, Herrero, Rolando, Strickler, Howard D., Viswanathan, Shankar, Nucci-Sack, Anne, Diaz, Angela, and Burk, Robert D.
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- 2022
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33. The lung microbiome, peripheral gene expression, and recurrence-free survival after resection of stage II non-small cell lung cancer
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Peters, Brandilyn A., Pass, Harvey I., Burk, Robert D., Xue, Xiaonan, Goparaju, Chandra, Sollecito, Christopher C., Grassi, Evan, Segal, Leopoldo N., Tsay, Jun-Chieh J., Hayes, Richard B., and Ahn, Jiyoung
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- 2022
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34. Impact of COVID-19 Mitigation Measures on Inner-City Female Youth in New York City
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Diaz, Angela, Nucci-Sack, Anne, Colon, Rachel, Guillot, Mary, Hollman, Dominic, Brunelli, Marie, Burk, Robert D., and Schlecht, Nicolas F.
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- 2022
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35. Menopause and Estrogen Associations With Gut Barrier, Microbial Translocation, and Immune Activation Biomarkers in Women With and Without HIV.
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Peters, Brandilyn A., Hanna, David B., Xiaonan Xue, Weber, Kathleen, Appleton, Allison A., Kassaye, Seble G., Topper, Elizabeth, Tracy, Russell P., Guillemette, Chantal, Caron, Patrick, Tien, Phyllis C., Qibin Qi, Burk, Robert D., Sharma, Anjali, Anastos, Kathryn, and Kaplan, Robert C.
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- 2024
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36. ICTV Virus Taxonomy Profile: Papillomaviridae
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Van Doorslaer, Koenraad, Chen, Zigui, Bernard, Hans-Ulrich, Chan, Paul KS, DeSalle, Rob, Dillner, Joakim, Forslund, Ola, Haga, Takeshi, McBride, Alison A, Villa, Luisa L, Burk, Robert D, and Consortium, ICTV Report
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Infection ,Animals ,Evolution ,Molecular ,Genome ,Viral ,Host Specificity ,Papillomaviridae ,ICTV Report ,taxonomy ,Biological Sciences ,Agricultural and Veterinary Sciences ,Medical and Health Sciences ,Virology - Abstract
The Papillomaviridae is a family of small, non-enveloped viruses with double-stranded DNA genomes of 5 748 to 8 607 bp. Their classification is based on pairwise nucleotide sequence identity across the L1 open reading frame. Members of the Papillomaviridae primarily infect mucosal and keratinised epithelia, and have been isolated from fish, reptiles, birds and mammals. Despite a long co-evolutionary history with their hosts, some papillomaviruses are pathogens of their natural host species. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of the Papillomaviridae, which is available at http://www.ictv.global/report/papillomaviridae.
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- 2018
37. Neighborhood Profiles and Body Mass Index Trajectory in Female Adolescents and Young Adults
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Niu, Li, Hoyt, Lindsay T., Pickering, Sarah, Nucci-Sack, Anne, Salandy, Anthony, Shankar, Viswanathan, Rodriguez, Elisa M., Burk, Robert D., Schlecht, Nicolas F., and Diaz, Angela
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- 2021
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38. Gut Microbiota and Blood Metabolites Related to Fiber Intake and Type 2 Diabetes
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Wang, Zheng, primary, Peters, Brandilyn A., additional, Yu, Bing, additional, Grove, Megan L., additional, Wang, Tao, additional, Xue, Xiaonan, additional, Thyagarajan, Bharat, additional, Daviglus, Martha L., additional, Boerwinkle, Eric, additional, Hu, Gang, additional, Mossavar-Rahmani, Yasmin, additional, Isasi, Carmen R., additional, Knight, Rob, additional, Burk, Robert D., additional, Kaplan, Robert C., additional, and Qi, Qibin, additional
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- 2024
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39. Dynamic risk prediction for cervical precancer screening with continuous and binary longitudinal biomarkers
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Roy, Siddharth, primary, Roy, Anindya, additional, Clarke, Megan A., additional, Gradissimo, Ana, additional, Burk, Robert D., additional, Wentzensen, Nicolas, additional, Albert, Paul S., additional, and Liu, Danping, additional
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- 2024
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40. Changes in cannabis, tobacco, and alcohol use among sexually active female adolescents and young adults over a twelve-year period ending in 2019
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Shyhalla, Kathleen, Smith, Danielle M., Diaz, Angela, Nucci-Sack, Anne, Guillot, Mary, Hollman, Dominic, Goniewicz, Maciej L., O'Connor, Richard J., Shankar, Viswanathan, Burk, Robert D., and Schlecht, Nicolas F.
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- 2021
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41. Integrated genomic and molecular characterization of cervical cancer
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Burk, Robert D, Chen, Zigui, Saller, Charles, Tarvin, Katherine, Carvalho, Andre L, Scapulatempo-Neto, Cristovam, Silveira, Henrique C, Fregnani, José H, Creighton, Chad J, Anderson, Matthew L, Castro, Patricia, Wang, Sophia S, Yau, Christina, Benz, Christopher, Robertson, A Gordon, Mungall, Karen, Lim, Lynette, Bowlby, Reanne, Sadeghi, Sara, Brooks, Denise, Sipahimalani, Payal, Mar, Richard, Ally, Adrian, Clarke, Amanda, Mungall, Andrew J, Tam, Angela, Lee, Darlene, Chuah, Eric, Schein, Jacqueline E, Tse, Kane, Kasaian, Katayoon, Ma, Yussanne, Marra, Marco A, Mayo, Michael, Balasundaram, Miruna, Thiessen, Nina, Dhalla, Noreen, Carlsen, Rebecca, Moore, Richard A, Holt, Robert A, Jones, Steven JM, Wong, Tina, Pantazi, Angeliki, Parfenov, Michael, Kucherlapati, Raju, Hadjipanayis, Angela, Seidman, Jonathan, Kucherlapati, Melanie, Ren, Xiaojia, Xu, Andrew W, Yang, Lixing, Park, Peter J, Lee, Semin, Rabeno, Brenda, Huelsenbeck-Dill, Lori, Borowsky, Mark, Cadungog, Mark, Iacocca, Mary, Petrelli, Nicholas, Swanson, Patricia, Ojesina, Akinyemi I, Le, Xuan, Sandusky, George, Adebamowo, Sally N, Akeredolu, Teniola, Adebamowo, Clement, Reynolds, Sheila M, Shmulevich, Ilya, Shelton, Candace, Crain, Daniel, Mallery, David, Curley, Erin, Gardner, Johanna, Penny, Robert, Morris, Scott, Shelton, Troy, Liu, Jia, Lolla, Laxmi, Chudamani, Sudha, Wu, Ye, Birrer, Michael, McLellan, Michael D, Bailey, Matthew H, Miller, Christopher A, Wyczalkowski, Matthew A, Fulton, Robert S, Fronick, Catrina C, Lu, Charles, Mardis, Elaine R, Appelbaum, Elizabeth L, Schmidt, Heather K, Fulton, Lucinda A, Cordes, Matthew G, Li, Tiandao, Ding, Li, Wilson, Richard K, Rader, Janet S, Behmaram, Behnaz, Uyar, Denise, and Bradley, William
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Biomedical and Clinical Sciences ,Oncology and Carcinogenesis ,Immunology ,Cervical Cancer ,Cancer ,Clinical Research ,Human Genome ,Sexually Transmitted Infections ,Biotechnology ,Genetics ,2.1 Biological and endogenous factors ,Aetiology ,APOBEC-1 Deaminase ,Adenocarcinoma ,B7-H1 Antigen ,Carcinoma ,Squamous Cell ,Caspase 8 ,DNA-Binding Proteins ,Female ,Genomics ,HLA-A Antigens ,Human papillomavirus 16 ,Humans ,Keratins ,Mitogen-Activated Protein Kinase Kinases ,Molecular Targeted Therapy ,Mutation ,Nuclear Proteins ,PTEN Phosphohydrolase ,Phosphatidylinositol 3-Kinases ,Programmed Cell Death 1 Ligand 2 Protein ,Protein Serine-Threonine Kinases ,Proteomics ,Proto-Oncogene Proteins p21(ras) ,RNA ,Long Noncoding ,Receptor ,ErbB-3 ,Receptor ,Transforming Growth Factor-beta Type II ,Receptors ,Transforming Growth Factor beta ,Signal Transduction ,Transcription Factors ,Uterine Cervical Neoplasms ,Virus Integration ,Cancer Genome Atlas Research Network ,Albert Einstein College of Medicine ,Analytical Biological Services ,Barretos Cancer Hospital ,Baylor College of Medicine ,Beckman Research Institute of City of Hope ,Buck Institute for Research on Aging ,Canada's Michael Smith Genome Sciences Centre ,Harvard Medical School ,Helen F. Graham Cancer Center &Research Institute at Christiana Care Health Services ,HudsonAlpha Institute for Biotechnology ,ILSbio ,LLC ,Indiana University School of Medicine ,Institute of Human Virology ,Institute for Systems Biology ,International Genomics Consortium ,Leidos Biomedical ,Massachusetts General Hospital ,McDonnell Genome Institute at Washington University ,Medical College of Wisconsin ,Medical University of South Carolina ,Memorial Sloan Kettering Cancer Center ,Montefiore Medical Center ,NantOmics ,National Cancer Institute ,National Hospital ,Abuja ,Nigeria ,National Human Genome Research Institute ,National Institute of Environmental Health Sciences ,National Institute on Deafness &Other Communication Disorders ,Ontario Tumour Bank ,London Health Sciences Centre ,Ontario Tumour Bank ,Ontario Institute for Cancer Research ,Ontario Tumour Bank ,The Ottawa Hospital ,Oregon Health &Science University ,Samuel Oschin Comprehensive Cancer Institute ,Cedars-Sinai Medical Center ,SRA International ,St Joseph's Candler Health System ,Eli &Edythe L. Broad Institute of Massachusetts Institute of Technology &Harvard University ,Research Institute at Nationwide Children's Hospital ,Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins University ,University of Bergen ,University of Texas MD Anderson Cancer Center ,University of Abuja Teaching Hospital ,University of Alabama at Birmingham ,University of California ,Irvine ,University of California Santa Cruz ,University of Kansas Medical Center ,University of Lausanne ,University of New Mexico Health Sciences Center ,University of North Carolina at Chapel Hill ,University of Oklahoma Health Sciences Center ,University of Pittsburgh ,University of São Paulo ,Ribeir ão Preto Medical School ,University of Southern California ,University of Washington ,University of Wisconsin School of Medicine &Public Health ,Van Andel Research Institute ,Washington University in St Louis ,Receptor ,erbB-3 ,General Science & Technology - Abstract
Cervical cancer remains one of the leading causes of cancer-related deaths worldwide. Here we report the extensive molecular characterization of 228 primary cervical cancers, one of the largest comprehensive genomic studies of cervical cancer to date. We observed notable APOBEC mutagenesis patterns and identified SHKBP1, ERBB3, CASP8, HLA-A and TGFBR2 as novel significantly mutated genes in cervical cancer. We also discovered amplifications in immune targets CD274 (also known as PD-L1) and PDCD1LG2 (also known as PD-L2), and the BCAR4 long non-coding RNA, which has been associated with response to lapatinib. Integration of human papilloma virus (HPV) was observed in all HPV18-related samples and 76% of HPV16-related samples, and was associated with structural aberrations and increased target-gene expression. We identified a unique set of endometrial-like cervical cancers, comprised predominantly of HPV-negative tumours with relatively high frequencies of KRAS, ARID1A and PTEN mutations. Integrative clustering of 178 samples identified keratin-low squamous, keratin-high squamous and adenocarcinoma-rich subgroups. These molecular analyses reveal new potential therapeutic targets for cervical cancers.
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- 2017
42. Kidney stone formation and the gut microbiome are altered by antibiotics in genetic hypercalciuric stone-forming rats
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Stern, Joshua M., Burk, Robert D., Asplin, John, Krieger, Nancy S., Suadicani, Sylvia O., Wang, Yi, Usyk, Mykhaylo, Lee, Justin A., Chen, Luojing, Becker, Jennifer, Chan, Michaela, and Bushinsky, David A.
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- 2021
- Full Text
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43. The association of medication use with clearance or persistence of oral HPV infection
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Lam, Jennifer O, Sugar, Elizabeth A, Cranston, Ross D, Weber, Kathleen M, Burk, Robert D, Wiley, Dorothy J, Reddy, Susheel, Margolick, Joseph B, Strickler, Howard D, Wentz, Alicia, Jacobson, Lisa, Coles, Christian L, Bream, Jay H, Rositch, Anne F, Guo, Yingshi, Xiao, Weihong, Gillison, Maura L, and D’Souza, Gypsyamber
- Subjects
Medical Microbiology ,Biomedical and Clinical Sciences ,Infectious Diseases ,Sexually Transmitted Infections ,Clinical Research ,Dental/Oral and Craniofacial Disease ,HIV/AIDS ,6.1 Pharmaceuticals ,Evaluation of treatments and therapeutic interventions ,Infection ,Adult ,Female ,HIV Infections ,Humans ,Longitudinal Studies ,Male ,Middle Aged ,Mouth Diseases ,Oropharyngeal Neoplasms ,Papillomaviridae ,Papillomavirus Infections ,Prevalence ,Risk Factors ,United States ,Oral HPV ,Prescription medication ,Clearance ,Antipsychotic ,HIV ,Immunomodulatory ,Oncology and Carcinogenesis ,Public Health and Health Services ,Epidemiology ,Oncology and carcinogenesis - Abstract
PurposePersistent oral human papillomavirus (HPV) infection increases risk for oropharyngeal carcinoma, and people living with HIV have higher rates of oral HPV infection and related cancers. Some prescription medications have immunomodulatory effects, but the impact of medication use on oral HPV natural history is unknown.MethodsScope® oral rinse-and-gargle samples were collected semi-annually from 1,666 participants and tested for 37 types of oral HPV DNA using PCR; 594 HPV-infected participants with 1,358 type-specific oral HPV infections were identified. Data were collected on recent (past 6 months) use of medications. The relationship between medication use and oral HPV clearance was evaluated using Wei-Lin-Weissfeld regression, adjusting for biologic sex, prevalent versus incident infection, age, HIV status and CD4+ T cell count.ResultsOut of 11 medications examined, oral HPV clearance was significantly reduced in participants reporting recent use of antipsychotics (HR 0.75, 95% CI 0.57-0.99), anxiolytics/sedatives (HR 0.78, 95% CI 0.63-0.96) and antidepressants (HR 0.82, 95% CI 0.67-0.999). Among antipsychotics users, effect modification by HIV status was observed, with reduced clearance in HIV-infected (HR 0.67, 95% CI 0.49-0.91), but not HIV-uninfected participants (p-interaction = 0.009). After adjusted analysis, antipsychotic use remained significantly associated with reduced oral HPV clearance overall (aHR 0.75, 95% CI 0.57-0.99), and when restricted to only HIV-infected participants (aHR 0.66, 95% CI 0.48-0.90). After adjustment, anxiolytic/sedative use and antidepressant use were no longer significantly associated with reduced oral HPV clearance.ConclusionsSome medications were associated with decreased oral HPV clearance, most notably antipsychotic medications. These medications are prescribed for conditions that may have immunomodulating effects, so characteristics of underlying illness may have partially contributed to reduced oral HPV clearance.
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- 2016
44. The Cervicovaginal Microbiota and Its Associations With Human Papillomavirus Detection in HIV-Infected and HIV-Uninfected Women
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Reimers, Laura L, Mehta, Supriya D, Massad, L Stewart, Burk, Robert D, Xie, Xianhong, Ravel, Jacques, Cohen, Mardge H, Palefsky, Joel M, Weber, Kathleen M, Xue, Xiaonan, Anastos, Kathryn, Minkoff, Howard, Atrio, Jessica, D'Souza, Gypsyamber, Ye, Qian, Colie, Christine, Zolnik, Christine P, Spear, Gregory T, and Strickler, Howard D
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Medical Microbiology ,Biomedical and Clinical Sciences ,Immunology ,Infectious Diseases ,Genetics ,HIV/AIDS ,Clinical Research ,Sexually Transmitted Infections ,Aetiology ,2.2 Factors relating to the physical environment ,Infection ,Good Health and Well Being ,Adult ,CD4 Lymphocyte Count ,CD4-Positive T-Lymphocytes ,Cervix Uteri ,Cohort Studies ,DNA ,Viral ,Female ,HIV Infections ,Humans ,Lactobacillus ,Microbiota ,Papillomaviridae ,RNA ,Ribosomal ,16S ,Vagina ,Vaginosis ,Bacterial ,HIV ,HPV ,human papillomavirus ,microbiota ,L ,crispatus ,Lactobacillus species ,L. crispatus ,Biological Sciences ,Medical and Health Sciences ,Microbiology ,Biological sciences ,Biomedical and clinical sciences ,Health sciences - Abstract
Background Bacterial vaginosis (BV) is characterized by low abundance of Lactobacillus species, high pH, and immune cell infiltration and has been associated with an increased risk of human papillomavirus (HPV) infection. We molecularly assessed the cervicovaginal microbiota over time in human immunodeficiency virus (HIV)-infected and HIV-uninfected women to more comprehensively study the HPV-microbiota relationship, controlling for immune status.Methods 16S ribosomal RNA gene amplicon pyrosequencing and HPV DNA testing were conducted annually in serial cervicovaginal lavage specimens obtained over 8-10 years from African American women from Chicago, of whom 22 were HIV uninfected, 22 were HIV infected with a stable CD4+ T-cell count of > 500 cells/mm3, and 20 were HIV infected with progressive immunosuppression. Vaginal pH was serially measured.Results The relative abundances of Lactobacillus crispatus and other Lactobacillus species were inversely associated with vaginal pH (all P < .001). High (vs low) L. crispatus relative abundance was associated with decreased HPV detection (odds ratio, 0.48; 95% confidence interval, .24-.96; Ptrend = .03) after adjustment for repeated observation and multiple covariates, including pH and study group. However, there were no associations between HPV and the relative abundance of Lactobacillus species as a group, nor with Lactobacillus gasseri, Lactobacillus iners, and Lactobacillus jensenii individually.Conclusions L. crispatus may have a beneficial effect on the burden of HPV in both HIV-infected and HIV-uninfected women (independent of pH).
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- 2016
45. The interaction between pubertal timing and childhood maltreatment on the risk of human papillomavirus infection among adolescent girls and young women
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Niu, Li, Hoyt, Lindsay Till, Salandy, Anthony, Nucci-Sack, Anne, Shankar, Viswanathan, Strickler, Howard, Burk, Robert D., Schlecht, Nicolas F., and Diaz, Angela
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- 2020
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46. A study of type-specific HPV natural history and implications for contemporary cervical cancer screening programs
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Demarco, Maria, Hyun, Noorie, Carter-Pokras, Olivia, Raine-Bennett, Tina R., Cheung, Li, Chen, Xiaojian, Hammer, Anne, Campos, Nicole, Kinney, Walter, Gage, Julia C., Befano, Brian, Perkins, Rebecca B., He, Xin, Dallal, Cher, Chen, Jie, Poitras, Nancy, Mayrand, Marie-Helene, Coutlee, Francois, Burk, Robert D., Lorey, Thomas, Castle, Philip E., Wentzensen, Nicolas, and Schiffman, Mark
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- 2020
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47. Effect of child abuse and neglect on risk behaviors in inner-city minority female adolescents and young adults
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Diaz, Angela, Shankar, Viswanathan, Nucci-Sack, Anne, Linares, Lourdes Oriana, Salandy, Anthony, Strickler, Howard D., Burk, Robert D., and Schlecht, Nicolas F.
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- 2020
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48. Cross-protection of the Bivalent Human Papillomavirus (HPV) Vaccine Against Variants of Genetically Related High-Risk HPV Infections
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Harari, Ariana, Chen, Zigui, Rodríguez, Ana Cecilia, Hildesheim, Allan, Porras, Carolina, Herrero, Rolando, Wacholder, Sholom, Panagiotou, Orestis A, Befano, Brian, Burk, Robert D, Schiffman, Mark, González, Paula, Herrero, Roland, Jiménez, Silvia E, Kreimer, Aimée R, Lowy, Douglas R, Schiller, John T, Sherman, Mark, Pinto, Ligia A, Kemp, Troy J, Sidawy, Mary, Quint, Wim, van Doorn, Leen-Jan, Struijk, Linda, Palefsky, Joel M, Darragh, Teresa M, and Stoler, Mark H
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HIV/AIDS ,Immunization ,HPV and/or Cervical Cancer Vaccines ,Cervical Cancer ,Vaccine Related ,Cancer ,Infectious Diseases ,Prevention ,Genetics ,Sexually Transmitted Infections ,Aetiology ,2.1 Biological and endogenous factors ,Infection ,Adolescent ,Adult ,Double-Blind Method ,Female ,Genetic Variation ,Humans ,Papillomaviridae ,Papillomavirus Infections ,Papillomavirus Vaccines ,Young Adult ,Costa Rica HPV Vaccine Trial Group ,HPV vaccine ,HPV variants ,cross-protection ,Biological Sciences ,Medical and Health Sciences ,Microbiology - Abstract
BackgroundResults from the Costa Rica Vaccine Trial (CVT) demonstrated partial cross-protection by the bivalent human papillomavirus (HPV) vaccine, which targets HPV-16 and HPV-18, against HPV-31, -33, and -45 infection and an increased incidence of HPV-51 infection.MethodsA study nested within the CVT intention-to-treat cohort was designed to assess high-risk HPV variant lineage-specific vaccine efficacy (VE). The 2 main end points were (1) long-term incident infections persisting for ≥2 years and/or progression to high-grade squamous intraepithelial lesions (ie, cervical intraepithelial neoplasia grade 2/3 [CIN 2/3]) and (2) incident transient infections lasting for
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- 2016
49. Association of cervical precancer with human papillomavirus types other than 16 among HIV co-infected women
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Massad, L Stewart, Xie, Xianhong, Burk, Robert D, D'Souza, Gypsyamber, Darragh, Teresa M, Minkoff, Howard, Colie, Christine, Burian, Pamela, Palefsky, Joel, Atrio, Jessica, and Strickler, Howard D
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Medical Microbiology ,Reproductive Medicine ,Biomedical and Clinical Sciences ,Immunology ,Infectious Diseases ,HIV/AIDS ,Prevention ,Cervical Cancer ,Clinical Research ,Sexually Transmitted Infections ,Cancer ,Immunization ,Infection ,Good Health and Well Being ,Adult ,Aged ,Coinfection ,Female ,Follow-Up Studies ,HIV Infections ,Human papillomavirus 16 ,Humans ,Incidence ,Logistic Models ,Middle Aged ,Papillomavirus Infections ,Uterine Cervical Dysplasia ,Uterine Cervical Neoplasms ,cervical intraepithelial neoplasia ,HIV in women ,human papillomavirus ,viral oncogenesis ,Paediatrics and Reproductive Medicine ,Obstetrics & Reproductive Medicine ,Reproductive medicine - Abstract
BackgroundHIV-seropositive women face high risk for infection with oncogenic human papillomavirus (oncHPV) types, abnormal Pap test results, and precancer, but cervical cancer risk is only modestly increased. Human papillomavirus (HPV)16 is highly oncogenic but only weakly associated with HIV status and immunosuppression, suggesting HPV16 may have a greater innate ability to evade host immune surveillance than other oncHPV types, which in turn should result in a greater relative increase in the prevalence of other oncHPV types among women with cervical precancer.ObjectiveWe sought to assess whether the underrepresentation of HPV16 among HIV-seropositive relative to HIV-seronegative women remains among those with cervical precancers.Study designHIV-seropositive and HIV-seronegative women in the Women's Interagency HIV Study were screened for cervical intraepithelial neoplasia (CIN) grade ≥3 (CIN3(+)). DNA from >40 HPV types was detected by polymerase chain reaction in cervicovaginal lavage specimens obtained at the visit at which CIN3(+) was diagnosed.ResultsHPV16 was detected in 13 (62%) of 21 HIV-seronegative women with CIN3(+) but only 44 (29%) of 154 HIV-seropositive women with CIN3(+) (P = .01). The lower prevalence of HPV16 in CIN3(+) among HIV-seropositive women persisted after controlling for covariates (odds ratio [OR], 0.25; 95% confidence interval [CI], 0.08-0.78). The prevalence of other members of the HPV16-related alpha-9 oncHPV clade as a group was similar in HIV-infected and uninfected women with CIN3(+) (OR, 1.02; 95% CI, 0.53-1.94). The prevalence of non-alpha-9 oncHPV types was increased in HIV-seropositive vs HIV-seronegative women with CIN3(+) (OR, 3.9; 95% CI, 1.3-11.8).ConclusionThe previously demonstrated increase in CIN3(+) incidence among HIV-seropositive women is associated with lower HPV16 and higher non-alpha-9 oncHPV prevalence. This is consistent with prior reports that HIV has a weak effect on infection by HPV16 relative to other oncHPV and supports use of nonavalent HPV vaccine in HIV-seropositive women.
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- 2016
50. The menopause-related gut microbiome: associations with metabolomics, inflammatory protein markers, and cardiometabolic health in women with HIV
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Wang, Yi, primary, Sharma, Anjali, additional, Weber, Kathleen M., additional, Topper, Elizabeth, additional, Appleton, Allison A., additional, Gustafson, Deborah, additional, Clish, Clary B., additional, Kaplan, Robert C., additional, Burk, Robert D., additional, Qi, Qibin, additional, and Peters, Brandilyn A., additional
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- 2023
- Full Text
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