1. The Medicago truncatula hypermycorrhizal B9 mutant displays an altered response to phosphate and is more susceptible to Aphanomyces euteiches
- Author
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Truong, Hoai-Nam, Thalineau, Elise, Bonneau, Laurent, Fournier, Carine, Potin, Sophie, Balzergue, Sandrine, Tuinen, Diederik, Jeandroz, Sylvain, Morandi, Dominique, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Unité de recherche en génomique végétale (URGV), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Burgundy Regional Council [FABER 2009-9201AAO036500681, PARI Agrale 8], Ministere de l'Enseignement Superieur et de la Recherche [2013-32], Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté ( UBFC ), Institut de recherche, santé, environnement et travail ( Irset ), Université d'Angers ( UA ) -Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ) -École des Hautes Études en Santé Publique [EHESP] ( EHESP ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) -Université des Antilles ( UA ), POPS Transcriptomic Platform, Institut National de la Recherche Agronomique ( INRA ) -Université d'Évry-Val-d'Essonne ( UEVE ) -Université Paris-Saclay-Centre National de la Recherche Scientifique ( CNRS ) -Institute of Plant Sciences Paris-Saclay, and Centre National de la Recherche Scientifique (CNRS)-Université d'Évry-Val-d'Essonne (UEVE)-Institut National de la Recherche Agronomique (INRA)
- Subjects
arbuscular mycorrhiza ,Gene Expression Profiling ,fungi ,food and beverages ,Aphanomyces ,Plant Roots ,symbiosis ,Phosphates ,Anthocyanins ,Phenotype ,[ SDV.SA.AGRO ] Life Sciences [q-bio]/Agricultural sciences/Agronomy ,Gene Expression Regulation, Plant ,nutrients ,Mycorrhizae ,Medicago truncatula ,Mutation ,Cluster Analysis ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,Disease Susceptibility ,signalling ,transcriptome ,Plant Shoots ,Oligonucleotide Array Sequence Analysis ,Signal Transduction - Abstract
International audience; Inorganic phosphate (Pi) plays a key role in the development of arbuscular mycorrhizal (AM) symbiosis, which is favoured when Pi is limiting in the environment. We have characterized the Medicago truncatula hypermycorrhizal B9 mutant for its response to limiting (P/10) and replete (P2) Pi. On P2, mycorrhization was significantly higher in B9 plants than in wild-type (WT). The B9 mutant displayed hallmarks of Pi-limited plants, including higher levels of anthocyanins and lower concentrations of Pi in shoots than WT plants. Transcriptome analyses of roots of WT and B9 plants cultivated on P2 or on P/10 confirmed the Pi-limited profile of the mutant on P2 and highlighted its altered response to Pi on P/10. Furthermore, the B9 mutant displayed a higher expression of defence/stress-related genes and was more susceptible to infection by the root oomycete pathogen Aphanomyces euteiches than WT plants. We propose that the hypermycorrhizal phenotype of the B9 mutant is linked to its Pi-limited status favouring AM symbiosis in contrast to WT plants in Pi-replete conditions, and discuss the possible links between the altered response of the B9 mutant to Pi, mycorrhization and infection by A. euteiches. We have characterized the response to phosphate of the hypermycorrhizal mutant B9 of Medicago truncatula. We show through physiological approaches, root architecture analyses and transcriptomics that this mutant displays an altered response to phosphate. We propose that the hypermycorrhizal phenotype of the B9 mutant is linked to its Pi-limited status favoring AM symbiosis. In addition this mutant is more susceptible to the oomycete Aphanomyces euteiches, suggesting possible links between symbiosis, pathogenesis and response to phosphate.
- Published
- 2014