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10. A general method for selection of riboflavin-overproducing food grade micro-organisms

Author

Rutten Ger, Smid Eddy J, Burgess Catherine M, and van Sinderen Douwe

Subjects
Microbiology, QR1-502
Abstract

Abstract Background This study describes a strategy to select and isolate spontaneous riboflavin-overproducing strains of Lactobacillus (Lb.) plantarum, Leuconostoc (Lc.) mesenteroides and Propionibacterium (P.) freudenreichii. Results The toxic riboflavin analogue roseoflavin was used to isolate natural riboflavin-overproducing variants of the food grade micro-organisms Lb. plantarum, Lc. mesenteroides and P. freudenreichii strains. The method was successfully employed for strains of all three species. The mutation(s) responsible for the observed overproduction of riboflavin were identified for isolates of two species. Conclusion Selection for spontaneous roseoflavin-resistant mutants was found to be a reliable method to obtain natural riboflavin-overproducing strains of a number of species commonly used in the food industry. This study presents a convenient method for deriving riboflavin-overproducing strains of bacterial starter cultures, which are currently used in the food industry, by a non-recombinant methodology. Use of such starter strains can be exploited to increase the vitamin content in certain food products.

Published
2006
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25. Salts and Salt Replacers

Author

Inguglia, Elena S., primary, Kerry, Joseph P., additional, Burgess, Catherine M., additional, and Tiwari, Brijesh K., additional

Published
2019
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34. Critically important antimicrobial resistant Enterobacteriaceae in Irish farm effluent and their removal in integrated constructed wetlands

Author

Prendergast, Deirdre M., primary, O'Doherty, Áine, additional, Burgess, Catherine M., additional, Howe, Nicole, additional, McMahon, Frederick, additional, Murphy, Declan, additional, Leonard, Finola, additional, Morris, Dearbháile, additional, Harrington, Caolan, additional, Carty, Aila, additional, Moriarty, John, additional, and Gutierrez, Montserrat, additional

Published
2022
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43. Impact of beef extract used for sample concentration on the detection of Escherichia coli DNA in water samples via qPCR

Author

Machado-Moreira, Bernardino, Machado-Moreira, Bernardino, Monteiro, Silvia, Santos, Ricardo, Martinez-Murcia, Antonio, Rajković, Andreja, Šmigić, Nada, Richards, Karl G., Abram, Florence, Burgess, Catherine M., Machado-Moreira, Bernardino, Machado-Moreira, Bernardino, Monteiro, Silvia, Santos, Ricardo, Martinez-Murcia, Antonio, Rajković, Andreja, Šmigić, Nada, Richards, Karl G., Abram, Florence, and Burgess, Catherine M.

Abstract

There is increasing interest in methodologies for the simultaneous concentration and detection of multiple targets in individual samples. The aim of this study was to investigate the potential presence of E. coli DNA in beef extract powder used as part of a procedure to concentrate water samples for the simultaneous detection of bacteria, viruses and protozoa. DNA from E. coli was detected in five out of six beef extract lots tested, demonstrating the limitations of its inclusion when being used in assays that will be used for the detection of E. coli in water samples. Further work is required to clarify if this phenomenon also occurs for other microorganisms of interest in water.

Published
2020

44. The riboflavin transporter RibU in Lactococcus lactis: molecular characterization of gene expression and the transport mechanism

Author

Burgess, Catherine M., Slotboom, Dirk Jan, Geertsma, Eric R., Duurkens, Ria H., Poolman, Bert, and van Sinderen, Douwe

Subjects
Lactococcus -- Genetic aspects, Vitamin B2 -- Research, Gene expression -- Research, Genetic research, Biological sciences
Abstract

This study describes the characterization of the riboflavin transport protein RibU in the lactic acid bacterium Lactococcus lactis subsp, cremoris NZ9000. RibU is predicted to contain five membrane-spanning segments and is a member of a novel transport protein family, not described in the Transport Classification Database. Transcriptional analysis revealed that ribU transcription is downregulated in response to riboflavin and flavin mononucleotide (FMN), presumably by means of the structurally conserved RFN (riboflavin) element located between the transcription start site and the start codon. An L. lactis strain carrying a mutated ribU gene exhibits altered transcriptional control of the riboflavin biosynthesis operon ribGBAH in response to riboflavin and FMN and does not consume riboflavin from its growth medium. Furthermore, it was shown that radiolabeled riboflavin is not taken up by the ribU mutant strain, in contrast to the wild-type strain, directly demonstrating the involvement of RibU in riboflavin uptake. FMN and the toxic riboflavin analogue roseoflayin were shown to inhibit riboflavin uptake and are likely to be RibU substrates. FMN transport by RibU is consistent with the observed transcriptional regulation of the ribGBAH operon by external FMN. The presented transport data are consistent with a uniport mechanism for riboflavin translocation and provide the first detailed molecular and functional analysis of a bacterial protein involved in riboflavin transport.

Published
2006

46. Whole-genome epidemiology links phage-mediated acquisition of a virulence gene to the clonal expansion of a pandemic Salmonella enterica serovar Typhimurium clone

Author

Tassinari, Eleonora, primary, Bawn, Matt, additional, Thilliez, Gaetan, additional, Charity, Oliver, additional, Acton, Luke, additional, Kirkwood, Mark, additional, Petrovska, Liljana, additional, Dallman, Timothy, additional, Burgess, Catherine M., additional, Hall, Neil, additional, Duffy, Geraldine, additional, and Kingsley, Robert A., additional

Published
2020
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