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Für das Deutsche wird gemeinhin eine strikte V2-Beschränkung angenommen, die für deklarative Hauptsätze besagt, dass sich vor dem finiten Verb genau eine Konstituente befinden muss. In der Literatur werden häufig Beispiele angeführt, in denen sich zwei Konstituenten vor dem finiten Verb befinden und die somit gegen die V2-Beschränkung verstoßen. Diese syntaktische Konfiguration, so das Argument, führt zu Ungrammatikalität: (1) *Gestern Johann hat getanzt. (Roberts & Roussou 2002:137) Die Bewertung in (1) fußt jedoch nicht auf empirischer Evidenz, sondern spiegelt ein introspektives Urteil der Autor*innen wider. Daten zum tatsächlichen Sprachgebrauch zeigen, dass Sätze wie in (2) im Deutschen durchaus verwendet werden: (2) Aber immer alle sagen das. [BSa-OB, #16] Die Dissertation beschäftigt sich mit dem Status dieser V3-Deklarativsätze im Deutschen. Der Status wird aus drei einander ergänzenden Perspektiven auf Sprache untersucht: Sprachverwendung, Akzeptabilität und Verarbeitung. Hierzu werden Daten, die in einer Korpus-, einer Akzeptabilitäts- und einer Lesezeitstudie erhoben wurden, ausgewertet. Basierend auf den empirischen Befunden diskutiere ich V3-Modellierungen aus generativer Sicht und entwickle einen Modellierungsvorschlag aus konstruktionsgrammatischer Sicht. Die Arbeit zeigt, dass die Einbeziehung von nicht-standardsprachlichen Mustern wichtige Einblicke in die sprachliche Architektur gibt. Insbesondere psycholinguistisch gewonnene Daten als empirische Basis sind essenziell, um mentale sprachliche Prozesse zu verstehen und abbilden zu können. Die Analyse von V3 zeigt, dass solche Ansätze möglich und nötig sind, um Grammatikmodelle zu prüfen und weiterzuentwickeln. Untersuchungen dieser Art stellen Grammatikmodelle in Frage, die oft einer standardsprachlichen Tradition heraus erwachsen sind und nur einen Ausschnitt der sprachlichen Realität erfassen. V3-Sätze entpuppen sich nach dieser Analyse als Strukturen, die fester Bestandteil der Grammatik sind., German is usually considered to follow a strict V2-constraint. This means that exactly one constituent must precede the finite verb in declarative main clauses. There are many examples for sentences that exhibit two preverbal constituents in the literature, illustrating a violation of the V2-constraint. According to the literature, these configurations lead to ungrammatical structures. (1) *Gestern Johann hat getanzt. (Roberts & Roussou 2002:137) However, the evaluation in (1) is not based on empirical evidence but is introspective and thus might not reflect the linguistic reality. Empirical data from actual language use show that German speakers indeed use these kinds of sentences. (2) Aber immer alle sagen das. [BSa-OB, #16] The dissertation explores the status of these V3 declaratives in German, with ‘status’ comprising three complementary perspectives on language: language use, acceptability, and processing. To this end, I analyze data from three studies: a corpus study, an acceptability judgment study, and a reading time study. Based on the empirical evidence, I discuss existing analyses of V3 and V3-modeling from the generative perspective and develop an analysis taking a construction-based approach. The dissertation shows that including patterns from non-standard language allows for valuable insights into the architecture of language. In particular, psycholinguistic data as an empirical basis are essential to understand and model mental linguistic processes. The analyses presented in the dissertation show that it is possible to follow such an approach in the field of syntactic variation, and it is indeed necessary in order to challenge and further develop existing grammatical theories and our understanding of grammar. Most grammatical models strongly rely on standard language, which is why they only capture a snippet of the linguistic reality. Taking empirical evidence into account, however, V3 sentences turn out to form an integral part of the German grammar.

For many scientific questions gaining three-dimensional insight into a specimen can provide valuable information. We here present an instrument called “tOMography Nano crYo (OMNY),” dedicated to high resolution 3D scanning x-ray microscopy at cryogenic conditions via hard X-ray ptychography. Ptychography is a lens-less imaging method requiring accurate sample positioning. In OMNY, this in achieved via dedicated laser interferometry and closed-loop position control reaching sub-10 nm positioning accuracy. Cryogenic sample conditions are maintained via conductive cooling. 90 K can be reached when using liquid nitrogen as coolant, and 10 K is possible with liquid helium. A cryogenic sample-change mechanism permits measurements of cryogenically fixed specimens. We compare images obtained with OMNY with older measurements performed using a nitrogen gas cryo-jet of stained, epoxy-embedded retina tissue and of frozen-hydrated Chlamydomonas cells., Review of Scientific Instruments, 89 (4), ISSN:0034-6748, ISSN:1089-7623

Although the organization of bone ultrastructure, i.e. the orientation and arrangement of the mineralized collagen fibrils, has been in the focus of research for many years for cortical bone, and many models on the osteonal arrangement have been proposed, limited attention has been paid to trabecular bone ultrastructure. This is surprising because trabeculae play a crucial role for the mechanical strength of several bone sites, including the vertebrae and the femoral head. On this account, we first validated a recently developed method (3D sSAXS or 3D scanning small-angle X-ray scattering) for investigating bone ultrastructure in a quantitative and spatially resolved way, using conventional linearly polarized light microscopy as a gold standard. While both methods are used to analyze thin tissue sections, in contrast to polarized light microscopy, 3D sSAXS has the important advantage that it provides 3D information on the orientation and arrangement of bone ultrastructure. In this first study of its kind, we used 3D sSAXS to investigate the ultrastructural organization of 22 vertebral trabeculae of different alignment, types and sizes, obtained from 4 subjects of different ages. Maps of ultrastructure orientation and arrangement of the trabeculae were retrieved by stacking information from consecutive 20-μm-thick bone sections. The organization of the ultrastructure was analyzed in relation to trabecular microarchitecture obtained from computed tomography and to relevant parameters such as distance to trabecular surface, local curvature or local bone mineralization. We found that (i) ultrastructure organization is similar for all investigated trabeculae independent of their particular characteristics, (ii) bone ultrastructure exhibiting a high degree of orientation was arranged in domains, (iii) highly oriented ultrastructural areas were located closer to the bone surface, (iv) the ultrastructure of the human trabecular bone specimens followed the microarchitecture, being oriented mostly parallel to bone surface, and (v) local surface curvature seems to have an effect on the ultrastructure organization. Further studies that investigate bone ultrastructure orientation and arrangement are needed in order to understand its organization and consequently its relation to bone biology and mechanics., PLoS ONE, 11 (8), ISSN:1932-6203

Biophysical Journal, 106 (2), ISSN:0006-3495, ISSN:1542-0086