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6 results on '"Bumbarger D"'

1. Three known species of the genus Cervidellus Thorne, 1937 (Rhabditida: Cephalobidae) from the south-western part of North America

Author

Holovachov, O.V., Mundo-Ocampo, M., Bostrom, S., and Bumbarger, D.

Subjects
Life Science, Laboratory of Nematology, PE&RC, Laboratorium voor Nematologie
Abstract

Three species of the genus Cervidellus, all collected in the south-western part of North America are described, i.e. C. cervus, C. doorsselaeri and C. capraeolus. The material of C. cervus agrees in most respects with the original description, although the females are shorter and the males are on average longer than the type specimens; however, the unique structure of the labial probolae is a strong indication of the conspecifi city of the two populations. The specimens of C. doorsselaeri agree well with the original description, but differ from the type population by a longer body, larger c-ratio; smaller c’-ratio; and a more posterior position of excretory pore, nerve ring and deirid in percentage of pharynx length. The specimens of C. capraeolus agree well with the original description, but differ from the type material by a more posterior position of excretory pore, nerve ring and deirid in percentage of neck length; another character separating the specimens of the two populations is the presence of tines on the inside of the primary branches of labial probolae in the Californian specimens.

Published
2007

2. Integrating EM and Patch-seq data: Synaptic connectivity and target specificity of predicted Sst transcriptomic types.

Author

Gamlin CR, Schneider-Mizell CM, Mallory M, Elabbady L, Gouwens N, Williams G, Mukora A, Dalley R, Bodor A, Brittain D, Buchanan J, Bumbarger D, Kapner D, Kinn S, Mahalingam G, Seshamani S, Takeno M, Torres R, Yin W, Nicovich PR, Bae JA, Castro MA, Dorkenwald S, Halageri A, Jia Z, Jordan C, Kemnitz N, Lee K, Li K, Lu R, Macrina T, Mitchell E, Mondal SS, Mu S, Nehoran B, Popovych S, Silversmith W, Turner NL, Wong W, Wu J, Yu S, Berg J, Jarsky T, Lee B, Seung HS, Zeng H, Reid RC, Collman F, da Costa NM, and Sorensen SA

Abstract

Neural circuit function is shaped both by the cell types that comprise the circuit and the connections between those cell types 1 . Neural cell types have previously been defined by morphology 2, 3 , electrophysiology 4, 5 , transcriptomic expression 6-8 , connectivity 9-13 , or even a combination of such modalities 14-16 . More recently, the Patch-seq technique has enabled the characterization of morphology (M), electrophysiology (E), and transcriptomic (T) properties from individual cells 17-20 . Using this technique, these properties were integrated to define 28, inhibitory multimodal, MET-types in mouse primary visual cortex 21 . It is unknown how these MET-types connect within the broader cortical circuitry however. Here we show that we can predict the MET-type identity of inhibitory cells within a large-scale electron microscopy (EM) dataset and these MET-types have distinct ultrastructural features and synapse connectivity patterns. We found that EM Martinotti cells, a well defined morphological cell type 22, 23 known to be Somatostatin positive (Sst+) 24, 25 , were successfully predicted to belong to Sst+ MET-types. Each identified MET-type had distinct axon myelination patterns and synapsed onto specific excitatory targets. Our results demonstrate that morphological features can be used to link cell type identities across imaging modalities, which enables further comparison of connectivity in relation to transcriptomic or electrophysiological properties. Furthermore, our results show that MET-types have distinct connectivity patterns, supporting the use of MET-types and connectivity to meaningfully define cell types.

Published
2023
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3. Genetic evidence for pax-3 function in myogenesis in the nematode Pristionchus pacificus.

Author

Yi B, Bumbarger D, and Sommer RJ

Subjects
Alleles, Amino Acid Sequence, Animals, Bone and Bones metabolism, Genetic Variation, Models, Genetic, Molecular Sequence Data, Nematoda, Phylogeny, Sequence Homology, Amino Acid, Gene Expression Regulation, Developmental, Muscle Development genetics, Muscle, Skeletal metabolism, Muscles metabolism, Paired Box Transcription Factors metabolism
Abstract

PAX3 is a member of the PAX3/7 subfamily of the paired box proteins. In vertebrates, Pax3 is essential for skeletal myogenesis by activating a cascade of transcriptional events that are necessary and sufficient for skeletal myogenesis. Four related basic helix-loop-helix transcription factors, MyoD, Myf5, Mrf4, and Myogenin, are targets of PAX3 and serve as myogenic regulatory factors. Although the role of Pax3 in myogenesis is well studied in vertebrates, little is known about invertebrate PAX-3 proteins and myogenesis. Here, we took advantage of viable alleles of pax-3 in the nematode satellite model organism Pristionchus pacificus to investigate the function of PAX-3 in myogenesis. Two strong reduction-of-function alleles of Ppa-pax-3 show severe muscle-derived abnormalities and phalloidin staining indicates a disruption of body wall muscle patterning. Furthermore, we identified a myogenic regulatory factor-related gene Ppa-hlh-1/MyoD and a serum response factor-related gene Ppa-unc-120. Expression of both genes in Ppa-pax-3 mutant animals is down regulated suggesting that Ppa-pax-3 acts upstream in the regulatory network. Together, our results provide the first genetic evidence for a conserved function of PAX-3 in myogenesis between vertebrates and nematodes.

Published
2009
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4. Phylogeny of Cephalobina (Nematoda): molecular evidence for recurrent evolution of probolae and incongruence with traditional classifications.

Author

Nadler SA, De Ley P, Mundo-Ocampo M, Smythe AB, Patricia Stock S, Bumbarger D, Adams BJ, De Ley IT, Holovachov O, and Baldwin JG

Subjects
Animals, Nematoda anatomy & histology, Evolution, Molecular, Nematoda classification, Nematoda genetics, Phylogeny
Abstract

Nematodes of the suborder Cephalobina include an ecologically and morphologically diverse array of species that range from soil-dwelling microbivores to parasites of vertebrates and invertebrates. Despite a long history of study, certain of these microbivores (Cephaloboidea) present some of the most intractable problems in nematode systematics; the lack of an evolutionary framework for these taxa has prevented the identification of natural groups and inhibited understanding of soil biodiversity and nematode ecology. Phylogenetic analyses of ribosomal (LSU) sequence data from 53 taxa revealed strong support for monophyly of taxa representing the Cephaloboidea, but do not support the monophyly of most genera within this superfamily. Historically these genera have primarily been recognized based on variation in labial morphology, but molecular phylogenies show the same general labial (probolae) morphotype often results from recurrent similarity, a result consistent with the phenotypic plasticity of probolae previously observed for some species in ecological time. Phylogenetic analyses of LSU rDNA also recovered strong support for some other groups of cephalobs, including taxa representing most (but not all) Panagrolaimoidea. In addition to revealing homoplasy of probolae, molecular trees also imply other unexpected patterns of character evolution or polarity, including recurrent similarity of offset spermatheca presence, and representation of complex probolae as the ancestral condition within Cephaloboidea. For Cephalobidae, molecular trees do not support traditional genera as natural groups, but it remains untested if deconstructing probolae morphotypes or other structural features into finer component characters may reveal homologies that help delimit evolutionary lineages.

Published
2006
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5. Raiding for fun and profit.

Author

Bumbarger D and Sheraw S

Subjects
Humans, United States, Labor Unions, Nursing, Societies, Nursing
Published
1989

6. Raiding for fun and profit.

Author

Bumbarger D and Sheraw S

Subjects
Humans, Michigan, Workforce, Labor Unions, Nursing Service, Hospital, Nursing Staff, Hospital supply & distribution, Societies, Nursing
Published
1986

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