126 results on '"Bulati M."'
Search Results
2. Role of non-coding RNAs in age-related vascular cognitive impairment: An overview on diagnostic/prognostic value in Vascular Dementia and Vascular Parkinsonism
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Miceli, V., primary, Russelli, G., additional, Iannolo, G., additional, Gallo, A., additional, Lo Re, V., additional, Agnese, V., additional, Sparacia, G., additional, Conaldi, P.G., additional, and Bulati, M., additional
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- 2020
- Full Text
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3. The role of immune response in ageing and longevity. A focus on B cell compartment
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Bulati, M., Caruso, C., Candore, G., Colonna Romano Giuseppina, Accardi, G, Caruso, C, Bulati, M., Caruso, C., Candore, G., and Colonna Romano Giuseppina
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Settore MED/04 - Patologia Generale ,Ageing, B cells, Centenarian offspring, Immunosenescence, Longevity - Abstract
The improvement of the quality of life of elderly people is going to become a priority because of the continuous increase in the number of centenarians. This render the studies of the processes involved in ageing of critical importance. Centenarians are a widely accepted model of successful ageing, a complex process which is influenced by several biological, environmental and lifestyle factors, because they have reached the extreme limits of life span overcoming the major age-related diseases. In centenarians model, several aspects have been studied, as inflammation, immune system, genetics and metabolism, to understand the secret of their long survival. It has been proposed that centenarians are characterized by more efficient protective molecules and biochemical pathways, and show well preserved immune functions.
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- 2017
4. B cells and immunosenescence: a focus on IgG+IgD-CD27- (DN) B cells in aged humans
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Bulati, M., Buffa, S., Candore, G., Caruso, C., Dunn Walters, D., Pellicano', M., Yc, W., COLONNA ROMANO, G., Bulati, M, Buffa, S, Candore, G, Caruso, C, Dunn Walters, DK, Pellicanò, M, Wu, YC, and Colonna Romano, G.
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Settore MED/04 - Patologia Generale ,B cells, elderly - Abstract
Immunosenescence contributes to the decreased ability of the elderly to control infectious diseases, which is also reflected in their generally poor response to new antigens and vaccination. It is known that the T cell branch of the immune system is impaired in the elderly mainly due to expansion of memory/effector cells that renders the immune system less able to respond to new antigens. B lymphocytes are also impaired in the elderly in terms of their response to new antigens. In this paper we review recent work on B cell immunosenescence focusing our attention on memory B cells and a subset of memory B cells (namely IgG(+)IgD(-)CD27(-)) that we have demonstrated is increased in healthy elderly.
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- 2011
5. A study of serum immunoglobulin levels in elderly persons that provides new insights into B cell immunosenescence
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Listi F., Candore G., Modica M. A., Russo M., Di Lorenzo G., Esposito Pellitteri M., Colonna Romano G., Aquino A., Bulati M., Lio D., Caruso C., FRANCESCHI, CLAUDIO, Listi F., Candore G., Modica M.A., Russo M., Di Lorenzo G., Esposito-Pellitteri M., Colonna-Romano G., Aquino A., Bulati M., Lio D., Franceschi C., and Caruso C.
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- 2006
6. Le cellule T regolatrici CD4+CD25+ negli anziani sono correlate al background genetico dell’Il-10 ed hanno effetti sui linfociti CD8-CMV specifici
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COLONNA ROMANO, Giuseppina, AQUINO, Alessandra, LIO, Domenico, CRIVELLO, Antonino, CANDORE, Giuseppina, BULATI M, VITELLO S, CLESI G, CARUSO C., COLONNA ROMANO G, AQUINO A, BULATI M, VITELLO S, CLESI G, LIO D, CRIVELLO A, CANDORE G, and CARUSO C
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- 2005
7. Valutazione Dei Linfociti T Regolatori CD4+CD25+ (Treg) Nell’anziano
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COLONNA ROMANO G, BULATI M, VITELLO S, CLESI G, CARUSO C., AQUINO, Alessandra, SCIALABBA, Giuseppe, LIO, Domenico, CANDORE, Giuseppina, COLONNA ROMANO G, AQUINO A, BULATI M, VITELLO S, CLESI G, SCIALABBA G, LIO D, CANDORE G, and CARUSO C
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- 2004
8. CD4+CD25+ regulatory cells in aged people are related to IL-10 genetic background and have effect on CMV-specific CD8 lymphocytes
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COLONNA ROMANO, Giuseppina, AQUINO, Alessandra, CRIVELLO, Antonino, BULATI M, CIACCIO MC, VITELLO S, LIO, Domenico, CANDORE G, CARUSO C., COLONNA ROMANO G, AQUINO A, BULATI M, CIACCIO MC, VITELLO S, LIO D, CRIVELLO A, CANDORE G, and CARUSO C
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- 2004
9. Evidence for Less Marked Potential Signs of T-Cell Immunosenescence in Centenarian Offspring Than in the General Age-Matched Population
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Pellicano, M., primary, Buffa, S., additional, Goldeck, D., additional, Bulati, M., additional, Martorana, A., additional, Caruso, C., additional, Colonna-Romano, G., additional, and Pawelec, G., additional
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- 2013
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10. B Cells Compartment in Centenarian Offspring and Old People
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Colonna-Romano, G., primary, Buffa, S., additional, Bulati, M., additional, Candore, G., additional, Lio, D., additional, Pellicano, M., additional, Vasto, S., additional, and Caruso, C., additional
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- 2010
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11. A Study of Serum Immunoglobulin Levels in Elderly Persons That Provides New Insights into B Cell Immunosenescence
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LISTI, F., primary, CANDORE, G., additional, MODICA, M. A., additional, RUSSO, M., additional, LORENZO, G. D., additional, ESPOSITO-PELLITTERI, M., additional, COLONNA-ROMANO, G., additional, AQUINO, A., additional, BULATI, M., additional, LIO, D., additional, FRANCESCHI, C., additional, and CARUSO, C., additional
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- 2006
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12. Age- and gender-related values of lymphocyte subsets in subjects from Northern and Southern Italy
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Colonna-Romano, G., primary, Cossarizza, A., additional, Aquino, A., additional, Scialabba, G., additional, Bulati, M., additional, Lio, D., additional, Candore, G., additional, Di Lorenzo, G., additional, Fradà, G., additional, and Caruso, C., additional
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- 2002
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13. Memory B cell subpopulations in the aged.
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Colonna-Romano G, Aquino A, Bulati M, Di Lorenzo G, Listì F, Vitello S, Lio D, Candore G, Clesi G, and Caruso C
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- 2006
14. The nACHR4 594C/T polymorphism in Alzheimer disease.
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Vasto S, Candore G, Aquino A, Bulati M, Balistreri CR, Grimaldi MP, Ditta V, Colonna-Romano G, Lio D, Vitello S, Barbiei R, and Caruso C
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- 2006
15. Immune-inflammatory responses in successful and unsuccessful ageing,Risposte immuno-infiammatorie nell'invecchiamento di e senza successo
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Candore, G., Carmela Rita Balistreri, Bulati, M., Colonna-Romano, G., Bona, D. D. I., Forte, G. I., Lio, D., Listì, F., Pellicanò, M., Scola, L., Vasto, S., and Caruso, C.
16. 176 - Mesenchymal Stem/Stromal Cells: HIGH-RESOLUTION MASS-SPECTROMETRY-BASED PROTEOMICS AND FUNCTIONAL VALIDATION REVEALED DISTINCT THERAPEUTIC CAPABILITIES RELATED TO DIFFERENT PRIMING STRATEGIES IN MESENCHYMAL STROMAL/STEM CELLS: POTENTIAL IMPLICATIONS FOR THEIR CLINICAL USE
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Miceli, V., Scilabra, S.D., Calligaris, M., Zito, G., Busà, R., Gallo, A., Bulati, M., and Conaldi, P.
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STROMAL cells , *STEM cells , *MESENCHYMAL stem cells - Published
- 2023
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17. Double Negative (CD19+IgG+IgD-CD27-) B Lymphocytes: A New Insight from Telomerase in Healthy Elderly, in Centenarian Offspring, and in Alzheimer’s Disease Patients
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Matteo Bulati, Cecilia Camarda, Delia Azzarello, Carmela Rita Balistreri, Calogero Caruso, Silvio Buffa, Giuseppina Colonna-Romano, Roberto Monastero, Adriana Martorana, Buffa, S, Martorana, A, Balistreri, CR, Bulati, M, Azzarello, DM, Camarda, C, Monastero, R, Caruso, C, Colonna Romano, G, Martorana A, Balistreri CR, Bulati M, Buffa S, Azzarello DM, Camarda C, Monastero R, Caruso C, and Colonna-Romano G
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Adult ,Telomerase ,Aging ,Immunology ,Population ,Naive B cell ,B-Lymphocyte Subsets ,Receptors, Antigen, B-Cell ,Centenarian offspring ,Lymphocyte Activation ,Severity of Illness Index ,CD19 ,Immunophenotyping ,Young Adult ,Alzheimer Disease ,medicine ,IgD-CD27- (Double Negative, DN) B cell population in the aged, DN B cell telomerase activity in young, elderly, CO and AD patients ,Immunology and Allergy ,Settore MED/05 - Patologia Clinica ,Humans ,education ,B cell ,Cellular Senescence ,Aged ,Inflammation ,Settore MED/04 - Patologia Generale ,Aged, 80 and over ,education.field_of_study ,CD40 ,biology ,B lymphocyte ,Age Factors ,TLR9 ,Immunosenescence ,Middle Aged ,medicine.anatomical_structure ,Phenotype ,Antigens, Surface ,biology.protein ,Alzheimer ,B lymphocytes ,Settore MED/26 - Neurologia ,Immunologic Memory - Abstract
Background: We have previously reported the increase of IgD-CD27- (Double Negative, DN) B cell population in the aged. These memory B cells have short telomeres and poor abilities to proliferate in vitro. Here, we investigated whether the low ability of DN B cells to proliferate depends on the expression levels of the CD307d and CD22 inhibitory receptors or whether DN B cells can proliferate and reactivate telomerase by the engagement of both innate and adaptive immune receptors. Methods: Phenotypic analyses were made by using flow cytometry. Quantitative analysis of telomerase activity was made by using a TRAP and a photometric enzyme immunoassay in young, healthy elderly, centenarian offspring (CO), and Alzheimer’s disease patients (AD). Results: We show that CD307d and CD22 expression levels are not related with the different ability of DN to be activated in the young and elderly. Moreover, CpG/α-IgG/α-CD40 (and not CpG or α-IgG/α-CD40) stimulation induces DN B cell proliferation in both young and elderly subjects. Furthermore, DN B cell telomerase activity in young, elderly, CO and AD patients, mirrors the age and health status of the subjects studied. Indeed, young donors show the highest levels of RTA, whereas AD patients the lowest. Healthy elderly and CO show lower levels than young, with a slight increase of activity in CO vs. elderly. Conclusions: Our present data add new information to our knowledge of DN B cells during aging. We demonstrate that the low ability of DN cells to proliferate depends on RTA and not on the expression of inhibitory receptors.
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- 2014
18. Neurological Screening in Elderly Liver Transplantation Candidates: A Single Center Experience
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Federica Avorio, Gianvincenzo Sparacia, Giovanna Russelli, Aurelio Seidita, Giuseppe Mamone, Rossella Alduino, Fabio Tuzzolino, Salvatore Gruttadauria, Roberto Miraglia, Matteo Bulati, Vincenzina Lo Re, Avorio F., Sparacia G., Russelli G., Seidita A., Mamone G., Alduino R., Tuzzolino F., Gruttadauria S., Miraglia R., Bulati M., and Lo Re V.
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cerebral small vessel disease ,liver transplantation ,brain magnetic resonance imaging elderly age-related diseases ,Liver transplantation ,Cerebral small vessel disease ,Neurology (clinical) ,Brain magnetic resonance imaging elderly age-related diseases - Abstract
Background: Cerebral small vessels disease (cSVD) is an age-related disorder and risk factor for stroke and cognitive/motor impairments. Neurological complications (NCs) are among the causes of adverse outcomes in older liver transplant recipients. This study sought to determine whether cSVD predicts acute NCs in over 65-year-old liver transplant patients. Methods: Data were collected, from a retrospective medical chart review, of 22 deceased donor liver transplant recipients aged 65 years or older with a pre-operative brain magnetic resonance imaging (MRI). We used the Fazekas score (0–3) as a quantitative measurement of the vascular lesion load seen in the MRI. We analyzed all post-operative acute NCs occurring during the hospital stay and any other non-NC. Results: cSVD was recognized in all patients. Neurological complications (NCs) occurred in 18.1% of patients with toxic-metabolic encephalopathy the most frequent diagnosis (13.64%). More severe cSVD was associated with seizures (p = 0.0362), longer hospital stay (p 0.0299), and disability (p 0.0134). In our elderly cohort, hepatic encephalopathy (HE) (p 0.0287) and ascites (p 0.0270) were predictors of NCs after liver transplantation. Ascites and/or variceal bleeding and severity of liver disease were associated with adverse post-operative outcomes. The small sample size limited the statistical analysis power. Conclusions: We present the preliminary data of a single-center retrospective study aimed at understanding the cSVD role on NCs and non-NCs after a liver transplantation in elderly patients. This would encourage a more appropriate multicenter prospective study that will definitely confirm if a neurological screening in old age liver transplant candidates is appropriate.
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- 2022
19. Changes in the Transcriptome Profiles of Human Amnion-Derived Mesenchymal Stromal/Stem Cells Induced by Three-Dimensional Culture: A Potential Priming Strategy to Improve Their Properties
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Alessia Gallo, Nicola Cuscino, Flavia Contino, Matteo Bulati, Mariangela Pampalone, Giandomenico Amico, Giovanni Zito, Claudia Carcione, Claudio Centi, Alessandro Bertani, Pier Giulio Conaldi, Vitale Miceli, Gallo A., Cuscino N., Contino F., Bulati M., Pampalone M., Amico G., Zito G., Carcione C., Centi C., Bertani A., Conaldi P.G., and Miceli V.
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QH301-705.5 ,Cell Culture Techniques ,Cell Separation ,Regenerative Medicine ,Article ,Catalysis ,Epigenesis, Genetic ,Immunophenotyping ,Inorganic Chemistry ,Humans ,Amnion ,Physical and Theoretical Chemistry ,Biology (General) ,Molecular Biology ,QD1-999 ,Spectroscopy ,Cells, Cultured ,Gene Expression Profiling ,Organic Chemistry ,Computational Biology ,RNA sequencing ,Cell Differentiation ,Mesenchymal Stem Cells ,Molecular Sequence Annotation ,General Medicine ,MSC therapeutic properties ,Computer Science Applications ,Chemistry ,Gene Ontology ,MSC spheroids ,Gene Expression Regulation ,human amnion-derived mesenchymal stromal/stem cells ,3D priming ,regenerative medicine ,Human amnion-derived mesenchymal stromal/stem cells ,Transcriptome ,Biomarkers - Abstract
Mesenchymal stromal/stem cells (MSCs) are believed to function in vivo as a homeostatic tool that shows therapeutic properties for tissue repair/regeneration. Conventionally, these cells are expanded in two-dimensional (2D) cultures, and, in that case, MSCs undergo genotypic/phenotypic changes resulting in a loss of their therapeutic capabilities. Moreover, several clinical trials using MSCs have shown controversial results with moderate/insufficient therapeutic responses. Different priming methods were tested to improve MSC effects, and three-dimensional (3D) culturing techniques were also examined. MSC spheroids display increased therapeutic properties, and, in this context, it is crucial to understand molecular changes underlying spheroid generation. To address these limitations, we performed RNA-seq on human amnion-derived MSCs (hAMSCs) cultured in both 2D and 3D conditions and examined the transcriptome changes associated with hAMSC spheroid formation. We found a large number of 3D culture-sensitive genes and identified selected genes related to 3D hAMSC therapeutic effects. In particular, we observed that these genes can regulate proliferation/differentiation, as well as immunomodulatory and angiogenic processes. We validated RNA-seq results by qRT-PCR and methylome analysis and investigation of secreted factors. Overall, our results showed that hAMSC spheroid culture represents a promising approach to cell-based therapy that could significantly impact hAMSC application in the field of regenerative medicine.
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- 2022
20. Amnion-Derived Mesenchymal Stromal/Stem Cell Paracrine Signals Potentiate Human Liver Organoid Differentiation: Translational Implications for Liver Regeneration
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Antonio Lo Nigro, Alessia Gallo, Matteo Bulati, Giampiero Vitale, Diego Sebastian Paini, Mariangela Pampalone, Daniele Galvagno, Pier Giulio Conaldi, Vitale Miceli, Lo Nigro A., Gallo A., Bulati M., Vitale G., Paini D.S., Pampalone M., Galvagno D., Conaldi P.G., and Miceli V.
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Medicine (General) ,Regeneration (biology) ,Mesenchymal stem cell ,General Medicine ,Biology ,Liver regeneration ,Transplantation ,Cell therapy ,3D liver organoid culture ,hepatocyte culture ,R5-920 ,Multipotent Stem Cell ,Cancer research ,Medicine ,hepatic progenitor cell differentiation ,Progenitor cell ,Stem cell ,liver regeneration ,human amnion-derived mesenchymal stem cells ,Original Research - Abstract
The prevalence of end-stage liver diseases has reached very high levels globally. The election treatment for affected patients is orthotopic liver transplantation, which is a very complex procedure, and due to the limited number of suitable organ donors, considerable research is being done on alternative therapeutic options. For instance, the use of cell therapy, such as the transplantation of hepatocytes to promote liver repair/regeneration, has been explored, but standardized protocols to produce suitable human hepatocytes are still limited. On the other hand, liver progenitor and multipotent stem cells offer potential cell sources that could be used clinically. Different studies have reported regarding the therapeutic effects of transplanted mesenchymal stromal/stem cells (MSCs) on end-stage liver diseases. Moreover, it has been shown that delivery of MSC-derived conditioned medium (MSC-CM) can reduce cell death and enhance liver proliferation in fulminant hepatic failure. Therefore, it is believed that MSC-CM contains many factors that probably support liver regeneration. In our work, we used an in vitro model of human liver organoids to study if the paracrine components secreted by human amnion-derived MSCs (hAMSCs) affected liver stem/progenitor cell differentiation. In particular, we differentiated liver organoids derived from bipotent EpCAM+ human liver cells and tested the effects of hAMSC secretome, derived from both two-dimensional (2D) and three-dimensional (3D) hAMSC cultures, on that model. Our analysis showed that conditioned medium (CM) produced by 3D hAMSCs was able to induce an over-expression of mature hepatocyte markers, such as ALB, NTCP, and CYP3A4, compared with both 2D hAMSC cultures and the conventional differentiation medium (DM). These data were confirmed by the over-production of ALB protein and over-activity of CYP3A4 observed in organoids grown in 3D hAMSC-CM. Liver repair dysfunction plays a role in the development of liver diseases, and effective repair likely requires the normal functioning of liver stem/progenitor cells. Herein, we showed that hAMSC-CM produced mainly by 3D cultures had the potential to increase hepatic stem/progenitor cell differentiation, demonstrating that soluble factors secreted by those cells are potentially responsible for the reaction. This work shows a potential approach to improve liver repair/regeneration also in a transplantation setting.
- Published
- 2021
21. The Immunomodulatory Properties of the Human Amnion-Derived Mesenchymal Stromal/Stem Cells Are Induced by INF-γ Produced by Activated Lymphomonocytes and Are Mediated by Cell-To-Cell Contact and Soluble Factors
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Matteo Bulati, Vitale Miceli, Alessia Gallo, Giandomenico Amico, Claudia Carcione, Mariangela Pampalone, Pier Giulio Conaldi, Bulati M., Miceli V., Gallo A., Amico G., Carcione C., Pampalone M., and Conaldi P.G.
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0301 basic medicine ,Programmed Cell Death 1 Receptor ,Cell Communication ,Lymphocyte Activation ,immunomodulation ,B7-H1 Antigen ,Monocytes ,0302 clinical medicine ,Immunology and Allergy ,Original Research ,Chemistry ,Cell Differentiation ,Healthy Volunteers ,I-kappa B Kinase ,Cell biology ,medicine.anatomical_structure ,primed-hAMSCs ,Monocyte differentiation ,Cytokines ,Stem cell ,lcsh:Immunologic diseases. Allergy ,Stromal cell ,T cell ,Primary Cell Culture ,Immunology ,regenerative medicine ,exosomes ,Interferon-gamma ,03 medical and health sciences ,Paracrine signalling ,Immune system ,interferon-γ ,medicine ,Humans ,Immunologic Factors ,Amnion ,human amnion-derived mesenchymal stem cells ,Cell Proliferation ,Immunosuppression Therapy ,PDL-1 ,Mesenchymal stem cell ,Immunity ,M2-like monocytes ,Mesenchymal Stem Cells ,Coculture Techniques ,Microvesicles ,MicroRNAs ,030104 developmental biology ,Leukocytes, Mononuclear ,lcsh:RC581-607 ,Interferon Regulatory Factor-1 ,030215 immunology - Abstract
Human mesenchymal stromal/stem cells (MSCs), being immunoprivileged and having immunomodulatory ability, represent a promising tool to be applied in the field of regenerative medicine. Based on numerous in vitro evidences, the immunological effects of MSCs on immune cells could depend on different mechanisms as cell-to-cell contact and paracrine signals. Furthermore, recent studies have shown that the immunomodulatory activity of MSCs is initiated by activated immune cells; thus, their interaction represents a potential homeostatic mechanism by which MSCs regulate the immune response. MSCs also release exosomes able to give different effects, in a paracrine manner, by influencing inflammatory processes. In this study, we aimed to establish the potential role of human amnion-derived MSCs (hAMSCs), in immunomodulation. We found that the immunosuppressive properties of hAMSCs are not constitutive, but require “supportive signals” capable of promoting these properties. Indeed, we observed that hAMSCs alone are not able to produce an adequate amount of soluble immunomodulatory factors. Here, we studied, in depth, the strong immunomodulatory licensing signal deriving from the direct interaction between hAMSCs and stimulated peripheral blood mononuclear cells. We found that the immunomodulatory effect of hAMSCs also depends on cell-to-cell contact through the contribution of the PDL-1/PD-1 axis. We then investigated the IFN-γ priming of hAMSCs (γ-hAMSCs), which induce the increase of PDL-1 expression, high production of IDO, and upregulation of different immunomodulatory exosome-derived miRNAs. Our miRNA–target network analysis revealed that nine of the deregulated miRNAs are involved in the regulation of key proteins that control both T cell activation/anergy and monocyte differentiation pathways. Finally, we observed that γ-hAMSCs induce in monocytes both M2-like phenotype and the increase of IL-10 production. The extensive implications of MSCs in modulating different aspects of the immune system make these cells attractive candidates to be employed in therapeutic application in immune-based diseases. For these reasons, we aimed, with this study, to shed light on the potential of hAMSCs, and how they could become a useful tool for treating different inflammatory diseases, including end-stage pathologies or adverse effects in transplanted patients.
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- 2020
22. Comparative study of the production of soluble factors in human placenta-derived mesenchymal stromal/stem cells grown in adherent conditions or as aggregates in a catheter-like device
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Vitale Miceli, Pier Giulio Conaldi, Mariangela Pampalone, Cinzia Maria Chinnici, Eva Schmelzer, Jörg C. Gerlach, Giandomenico Amico, Matteo Bulati, Miceli V., Chinnici C.M., Bulati M., Pampalone M., Amico G., Schmelzer E., Gerlach J.C., and Conaldi P.G.
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0301 basic medicine ,Stromal cell ,Angiogenesis ,Cell Survival ,Placenta ,Cell ,Biophysics ,Cell Culture Techniques ,Biocompatible Materials ,Biology ,Paracrine effects ,Biochemistry ,Regenerative medicine ,Immunomodulation ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Cell Movement ,Pregnancy ,medicine ,Cell Adhesion ,Human Umbilical Vein Endothelial Cells ,Humans ,Immunologic Factors ,Amnion ,Molecular Biology ,Cell Aggregation ,Settore MED/04 - Patologia Generale ,Catheter-like device ,Placenta-derived stromal/stem cells ,Settore BIO/16 - Anatomia Umana ,Mesenchymal stem cell ,Mesenchymal Stem Cells ,Cell Biology ,Cells, Immobilized ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Cancer research ,Angiogenesis Inducing Agents ,Female ,Stem cell ,Adult stem cell - Abstract
Different approaches have been studied in both preclinical and clinical settings to develop cell-based therapies and/or engineered cell-based therapies to better integrate grafts with the host. In these techniques, much attention is addressed to the use of adult stem cells such as mesenchymal stem cells (MSCs), but identifying and obtaining sufficient numbers of therapeutic cells, and the right route of administration, is often a challenge. In this study, we tested the feasibility of encapsulating human amnion-derived MSCs (hAMSCs) in a semipermeable and biocompatible fiber as a new approach for regenerative medicine. Our data showed that hAMSCs aggregated in the device constitutes an effective system for enhancing, or at least for maintaining, the paracrine activity of these cells in order to better promote tissue regeneration in an immune isolated state. In our new experimental approach, the hAMSCs retained their therapeutic potential, as shown by both the production of specific immunomodulatory/angiogenic factors and immunomodulatory and angiogenic ability observed in vitro. Unlike cell infusion methods, the use of encapsulated-cells leads to minimally invasive approaches, avoiding a direct interaction with the host. Therefore, the potentiality of an allograft or xenograft without the need for immunosuppression, and the lack of tumorigenesis is very intriguing.
- Published
- 2020
23. Role of non-coding RNAs in age-related vascular cognitive impairment: An overview on diagnostic/prognostic value in Vascular Dementia and Vascular Parkinsonism
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Alessia Gallo, Pier Giulio Conaldi, V. Lo Re, Vitale Miceli, Gianvincenzo Sparacia, Valentina Agnese, Matteo Bulati, Gioacchin Iannolo, Giovanna Russelli, Miceli V., Russelli G., Iannolo G., Gallo A., Lo Re V., Agnese V., Sparacia G., Conaldi P.G., and Bulati M.
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0301 basic medicine ,Aging ,RNA, Untranslated ,Endothelium ,Heart disease ,Vascular Parkinsonism ,Vascular Dementia ,Bioinformatics ,Pathogenesis ,03 medical and health sciences ,0302 clinical medicine ,Parkinsonian Disorders ,medicine ,Dementia ,Humans ,Cognitive Dysfunction ,Endothelial dysfunction ,Vascular dementia ,Stroke ,business.industry ,Parkinsonism ,Dementia, Vascular ,Vascular ageing ,medicine.disease ,ncRNA ,030104 developmental biology ,medicine.anatomical_structure ,Blood brain barrier ,Cerebrovascular Circulation ,business ,030217 neurology & neurosurgery ,Developmental Biology - Abstract
Age is the pivotal risk factor for different common medical conditions such as cardiovascular diseases, cancer and dementia. Among age-related disorders, cardiovascular and cerebrovascular diseases, represent the leading causes of premature mortality strictly related to vascular ageing, a pathological condition characterized by endothelial dysfunction, atherosclerosis, hypertension, heart disease and stroke. These features negatively impact on the brain, owing to altered cerebral blood flow, neurovascular coupling and impaired endothelial permeability leading to cerebrovascular diseases (CVDs) as Vascular Dementia (VD) and Parkinsonism (VP). It is an increasing opinion that neurodegenerative disorders and cerebrovascular diseases are associated from a pathogenetic point of view, and in this review, we discuss how cerebrovascular dysfunctions, due to epigenetic alterations, are linked with neuronal degeneration/dysfunction that lead to cognitive impairment. The relation between neurodegenerative and cerebrovascular diseases are reviewed with a focus on role of ncRNAs in age-related vascular diseases impairing the endothelium in the blood-brain barrier with consequent dysfunction of cerebral blood flow. In this review we dissert about different regulatory mechanisms of gene expression implemented by ncRNAs in the pathogenesis of age-related neurovascular impairment, aiming to highlight the potential use of ncRNAs as biomarkers for diagnostic/prognostic purposes as well as novel therapeutic targets.
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- 2020
24. From lymphopoiesis to plasma cells differentiation, the age-related modifications of B cell compartment are influenced by 'inflamm-ageing'
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Calogero Caruso, Matteo Bulati, Giuseppina Colonna-Romano, Bulati, M., Caruso, C., and Colonna-Romano, G.
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0301 basic medicine ,Aging ,Immunosenescence ,Health Status ,Plasma Cells ,Naive B cell ,Autoimmunity ,Inflammation ,Biology ,Lymphocyte Activation ,Biochemistry ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Antigen ,Age-related disease ,medicine ,Animals ,Humans ,Lymphopoiesis ,Progenitor cell ,Molecular Biology ,Cellular Senescence ,B cell ,B-Lymphocytes ,Cell Differentiation ,Inflamm-ageing ,030104 developmental biology ,medicine.anatomical_structure ,Neurology ,Immune System ,Immunology ,Inflammation Mediators ,medicine.symptom ,Exhausted/Senescent cell ,030215 immunology ,Biotechnology - Abstract
Ageing is a complex process characterized by a general decline in physiological functions with increasing morbidity and mortality. The most important aspect of ageing is the chronic inflammatory status, named âinflamm-ageingâ, strictly associated with the deterioration of the immune function, termed âimmunosenescenceâ. Both are causes of increased susceptibility of elderly to infectious diseases, cancer, dementia, cardiovascular diseases and autoimmunity, and of a decreased response to vaccination. It has been widely demonstrated that ageing has a strong impact on the remodelling of the B cell branch of immune system. The first evident effect is the significant decrease in circulating B cells, primarily due to the reduction of new B cell coming from bone marrow (BM) progenitors, as inflammation directly impacts on B lymphopoiesis. Besides, in aged individuals, there is a shift from naïve to memory immunoglobulins production, accompanied by the impaired ability to produce high affinity protective antibodies against newly encountered antigens. This is accompanied by the increase of expanded clones of B cells, which correlates with poor health status. Age-related modifications also occur in naïve/memory B cells subsets. Indeed, in the elderly, there is a reduction of naïve B cells, accompanied by the expansion of memory B cells that show a senescence-associated phenotype. Finally, elderly show the impaired ability of memory B cells to differentiate into plasma cells. It can be concluded that inflammation is the leading cause of the age-related impairment of B cell compartment, which play certainly a key role in the development of age-related diseases. This makes study of B cells in the aged an important tool for monitoring immunosenescence, chronic inflammatory disorders and the effectiveness of vaccines or pharmacological therapies.
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- 2017
25. Evidence for Less Marked Potential Signs of T-Cell Immunosenescence in Centenarian Offspring Than in the General Age-Matched Population
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Matteo Bulati, Adriana Martorana, Calogero Caruso, Graham Pawelec, Giuseppina Colonna-Romano, David Goldeck, Mariavaleria Pellicanò, Silvio Buffa, Pellicanò M, Buffa S, Goldeck D, Bulati M, Martorana A, Caruso C, Colonna-Romano G, and Pawelec G
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Adult ,Male ,Aging ,Immunosenescence ,Offspring ,Health Status ,T-Lymphocytes ,T cell ,media_common.quotation_subject ,Longevity ,Population ,CD4-CD8 Ratio ,T cells ,Biology ,Lymphocyte Activation ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Antigen ,medicine ,Humans ,education ,Aged ,030304 developmental biology ,media_common ,Aged, 80 and over ,Settore MED/04 - Patologia Generale ,0303 health sciences ,education.field_of_study ,Age Factors ,medicine.anatomical_structure ,Case-Control Studies ,Centenarian offspring ,Immunology ,Adult Children ,Female ,Geriatrics and Gerontology ,Centenarian ,030215 immunology - Abstract
People may reach the upper limits of the human life span at least partly because they have maintained more appropriate immune function, avoiding changes to immunity termed "immunosenescence." Exceptionally long-lived people may be enriched for genes that contribute to their longevity, some of which may bear on immune function. Centenarian offspring would be expected to inherit some of these, which might be reflected in their resistance to immunosenescence, and contribute to their potential longevity. We have tested this hypothesis by comparing centenarian offspring with age-matched controls. We report differences in the numbers and proportions of both CD4(+) and CD8(+) early- and late-differentiated T cells, as well as potentially senescent CD8(+) T cells, suggesting that the adaptive T-cell arm of the immune system is more "youthful" in centenarian offspring than controls. This might reflect a superior ability to mount effective responses against newly encountered antigens and thus contribute to better protection against infection and to greater longevity.
- Published
- 2013
26. Bone marrow B lymphocytes in multiple myeloma and MGUS: Focus on distribution of naïve cells and memory subsets
- Author
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Caterina Giambanco, Francesco Di Bassiano, Silvio Buffa, Calogero Caruso, Matteo Bulati, Giuseppina Colonna Romano, Francesco Gervasi, Fanny Pojero, Alessandra Casuccio, Pojero, F., Casuccio, A., Giambanco, C., Bulati, M., Buffa, S., Di Bassiano, F., Gervasi, F., Caruso, C., and Colonna Romano, G.
- Subjects
0301 basic medicine ,Male ,Cancer Research ,B-Lymphocyte Subsets ,chemical and pharmacologic phenomena ,Bone Marrow Cells ,Immunoglobulin D ,Monoclonal Gammopathy of Undetermined Significance ,Flow cytometry ,03 medical and health sciences ,Immune system ,stomatognathic system ,immune system diseases ,hemic and lymphatic diseases ,medicine ,Humans ,B cell ,Multiple myeloma ,B-Lymphocytes ,medicine.diagnostic_test ,biology ,hemic and immune systems ,Hematology ,medicine.disease ,Flow Cytometry ,Tumor Necrosis Factor Receptor Superfamily, Member 7 ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,Case-Control Studies ,Immunology ,biology.protein ,MGUS ,Female ,Bone marrow ,Multiple Myeloma ,Monoclonal gammopathy of undetermined significance - Abstract
Multiple myeloma (MM) is caused by proliferation of clonal plasma cells (cPCs) in bone marrow (BM), associated with numerical and functional defects in immune subsets. An impairment of B cell compartment is involved in onset/progression of the disease.By flow cytometry, we studied distribution of naïve/transitional (IgD(+)CD27(-)), memory unswitched (IgD(+)CD27(+)), memory switched (IgD(-)CD27(+)) and double negative (DN) (IgD(-)CD27(-)) B lymphocytes in BM of control subjects, and responding and relapsing patients.We observed an increased percentage of IgD(+)CD27(+) B cells in healthy controls vs responding patients (p0.05). Treated non complete responders exhibited an expanded DN compartment vs stringent complete responders (p=0.011); in turn IgD(+)CD27(-) subpopulation was larger in stringent complete responders vs other responding patients (p=0.006). None of the studied B cell subsets showed clonal restriction. Correlation analysis revealed negative correlations between naïve/transitional and DN B cells in all groups, except in newly diagnosed subjects.This may be considered a feasible start point to explore the importance of B cells in the immunosuppressive MM BM microenvironment, correlating these findings with immunosenescence and therapy related increased risk of infection. Moreover, we propose a possible role of naïve/transitional and DN B cells as predictive markers in treated patients.
- Published
- 2016
27. Immune profiling of Alzheimer patients
- Author
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Calogero Caruso, Graham Pawelec, David Goldeck, Anis Larbi, Giuseppina Colonna-Romano, Silvio Buffa, Matteo Bulati, Graziella Rubino, Evelyna Derhovanessian, Francesco Iemolo, Giuseppina Candore, Mariavaleria Pellicanò, Pellicanò, M, Larbi, A, Goldeck, D, Colonna-Romano, G, Buffa, S, Bulati, M, Rubino, G, Iemolo, F, Candore, G, Caruso, C, Derhovanessian, E, and Pawelec, G
- Subjects
Adult ,CD4-Positive T-Lymphocytes ,Male ,Immunosenescence ,T cell ,Immunology ,Stimulation ,Disease ,CD8-Positive T-Lymphocytes ,Biology ,Young Adult ,Alzheimer Disease ,Extracellular ,medicine ,Humans ,Immunology and Allergy ,Senile plaques ,Aged ,Aged, 80 and over ,Settore MED/04 - Patologia Generale ,Gene Expression Profiling ,Aβ42 ,Age Factors ,Cell Differentiation ,Middle Aged ,Alzheimer's disease ,Phenotype ,CD4 Lymphocyte Count ,medicine.anatomical_structure ,Neurology ,Etiology ,Female ,Neurology (clinical) ,Biomarkers - Abstract
Alzheimer's disease (AD) is characterized by extracellular senile plaques in the brain, containing amyloid-β peptide (Aβ). We identify immunological differences between AD patients and age-matched controls greater than those related to age itself. The biggest differences were in the CD4 + rather than the CD8 + T cell compartment resulting in lower proportions of naive cells, more late-differentiated cells and higher percentages of activated CD4 + CD25 + T cells without a Treg phenotype in AD patients. Changes to CD4 + cells might be the result of chronic stimulation by Aβ present in the blood. These findings have implications for diagnosis and understanding the aetiology of the disease.
- Published
- 2012
28. Systemic Immune Responses in Alzheimer's Disease: In Vitro Mononuclear Cell Activation and Cytokine Production
- Author
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Matteo Bulati, Anna Di Prima, Sonya Vasto, Mario Barbagallo, Mariavaleria Pellicanò, Silvio Buffa, Marta Di Carlo, Calogero Caruso, Gabriella Misiano, Domenico Lio, Pasquale Picone, Domenico Nuzzo, Giuseppina Colonna-Romano, Giuseppina Candore, Pellicanò, M, Bulati, M, Buffa, S, Barbagallo, M, Di Prima, A, Misiano, G, Pasquale Picone, P, Di Carlo, M, Nuzzo, D, Candore, G, Vasto, S, Lio, D, Caruso, C, and Colonna Romano, G
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Male ,Eotaxin ,CCR2 ,Chemokine ,Time Factors ,medicine.medical_treatment ,Peripheral blood mononuclear cell ,Chemokine receptor ,Immune system ,Alzheimer’s disease, chemokine, cytokine, PBMC, rAβ42 ,Alzheimer Disease ,medicine ,Humans ,Lymphocytes ,IL-2 receptor ,Cells, Cultured ,Aged ,Settore MED/04 - Patologia Generale ,Aged, 80 and over ,Analysis of Variance ,Amyloid beta-Peptides ,biology ,business.industry ,General Neuroscience ,General Medicine ,Middle Aged ,Flow Cytometry ,Peptide Fragments ,Psychiatry and Mental health ,Clinical Psychology ,Cytokine ,Gene Expression Regulation ,Case-Control Studies ,Immunology ,Leukocytes, Mononuclear ,biology.protein ,Cytokines ,Female ,Geriatrics and Gerontology ,business - Abstract
To investigate the systemic signs of immune-inflammatory responses in Alzheimer's disease (AD), in the present study we have analyzed blood lymphocyte subsets and the expression of activation markers on peripheral blood mononuclear cells (PBMCs) from AD patients and age-matched healthy controls (HC) activated in vitro by recombinant amyloid-beta peptide (rAbeta42). Our study of AD lymphocyte subpopulations confirms the already described decrease of the absolute number and percentage of B cells when compared to HC lymphocytes, whereas the other subsets are not significantly different in patients and controls. We report the increased expression of the activation marker CD69 and of the chemokine receptors CCR2 and CCR5 on T cells but no changes of CD25 after activation. B cells are also activated by rAbeta42 as demonstrated by the enhanced expression of CCR5. Moreover, rAbeta42 induces an increased expression of the scavenger receptor CD36 on monocytes. Some activation markers and chemokine receptors are overexpressed in unstimulated AD cells when compared to controls. This is evidence of the pro-inflammatory status of AD. Stimulation by rAbeta42 also induces the production of the pro-inflammatory cytokines IL-1beta, IL-6, IFN-gamma, and TNF-alpha, and of the anti-inflammatory cytokines IL-10 and IL-1Ra. The chemokines RANTES, MIP-1beta, and eotaxin as well as some growth factors (GM-CSF, G-CSF) are also overproduced by AD-derived PBMC activated by rAbeta42. These results support the involvement of systemic immunity in AD patients. However, our study is an observational one so we cannot draw a conclusion about its contribution to the pathophysiology of the disease.
- Published
- 2010
29. B Cells Compartment in Centenarian Offspring and Old People
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Giuseppina Colonna-Romano, Matteo Bulati, Mariavaleria Pellicanò, Sonya Vasto, Domenico Lio, Calogero Caruso, Silvio Buffa, Giuseppina Candore, Colonna Romano, G, Buffa, S, Bulati, M, Candore, G, Lio, D, Pellicanò, M, Vasto, S, and Caruso, C
- Subjects
Aging ,B lymphocyte, centenarian, immunosenescence, longevity ,Offspring ,T cell ,Longevity ,Naive B cell ,B-Lymphocyte Subsets ,Immunoglobulin D ,Drug Discovery ,medicine ,Humans ,B cell ,Aged ,Aged, 80 and over ,Settore MED/04 - Patologia Generale ,Pharmacology ,biology ,business.industry ,Immunosenescence ,Middle Aged ,Cell Compartmentation ,medicine.anatomical_structure ,Ageing ,Immunology ,biology.protein ,Adult Children ,Centenarian ,business - Abstract
Immunosenescence is considered a major contributory factor to the increased frequency of morbidity and mortality among elderly. On the other hand centenarians are considered the best example of successful ageing. To gain insight into mechanisms of immunosenescence and its clinical relevance, a possible model is represented by centenarians and/or their offspring. Nowadays centenarians are not more a curiosity, but in Europe are 1/8000 inhabitants and it has been demonstrated that the centenarian offspring, who are typically in their 70s and 80s, have a survival advantage when compared with age-matched controls whose parents died at an average life expectancy. Then again, studies on immunosenescence focus mainly on T cell impairment, although B cells are also affected. So, in the present preliminary report, we have studied B cell compartment in two classes of individuals, old people and centenarian offspring. B cell compartment was analysed using IgD and CD27 antibodies which characterize naïve B cells (IgD(+) CD27(-)), memory unswitched B cells (IgD(+)CD27(+)), memory switched B cells(IgD(-)CD27(+)) and double negative B cells (DN) (IgD(-)CD27(-)), i.e. exhausted memory cells. As expected, in both cohorts we observed a decreased B cell count. However, in centenarian offspring, naïve B cells are more abundant whereas exhausted memory cells (DN B cells, IgD(-)CD27(-)) do not show the increase that we have previously demonstrated in healthy elderly donors. These data are similar to that found in previously experiments on young subjects. So, our preliminary results show that centenarian offspring do not have the typical trend of memory/naive B cell subsets observed in elderly people and this is in agreement with the higher levels of IgM in the serum of centenarian offspring in comparison with data obtained in age-matched controls. This reservoir of naive B cell might be one of the causes that make centenarian offspring able to keep fighting off new infections, hence prolonging their life. So, B cell subset changes could represent a hallmark of successful or unsuccessful ageing and could be used as a biomarker of human life span, potentially useful for the evaluation of anti-ageing treatment.
- Published
- 2010
30. A double-negative (IgD−CD27−) B cell population is increased in the peripheral blood of elderly people
- Author
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Salvatore Vitello, Giuseppina Candore, Domenico Lio, Giuseppina Colonna-Romano, Calogero Caruso, Mariavaleria Pellicanò, Matteo Bulati, Alessandra Aquino, Colonna Romano, G, Bulati, M, Aquino, A, Pellicanò, M, Vitello, S, Lio, D, Candore, G, and Caruso, C
- Subjects
Adult ,Aging ,ATP Binding Cassette Transporter, Subfamily B ,T cell ,Antigens, CD19 ,B-Lymphocyte Subsets ,chemical and pharmacologic phenomena ,Young Adult ,B lymphocyte, Immunosenescence, IgD, CD27, Elderly, Immunologic memory ,medicine ,Humans ,Cytotoxic T cell ,ATP Binding Cassette Transporter, Subfamily B, Member 1 ,IL-2 receptor ,CD40 Antigens ,CD154 ,Antigen-presenting cell ,Cells, Cultured ,Aged ,Aged, 80 and over ,Settore MED/04 - Patologia Generale ,business.industry ,Age Factors ,HLA-DR Antigens ,Immunoglobulin D ,Middle Aged ,Telomere ,Flow Cytometry ,Acquired immune system ,Tumor Necrosis Factor Receptor Superfamily, Member 7 ,B-1 cell ,Ki-67 Antigen ,medicine.anatomical_structure ,Proto-Oncogene Proteins c-bcl-2 ,Immunology ,B7-1 Antigen ,business ,Immunologic Memory ,CD80 ,Developmental Biology - Abstract
The T cell branch of the immune system has been extensively studied in the elderly and it is known that the elderly have impaired immune function, mainly due to the chronic antigenic load that ultimately causes shrinkage of the T cell repertoire and filling of the immunologic space with memory T cells. In the present paper, we describe the IgD(-)CD27(-) double-negative B cell population which (as we have recently described) is higher in the elderly. Most of these cells were IgG(+). Evaluation of the telomere length and expression of the ABCB1 transporter and anti-apoptotic molecule, Bcl2, shows that they have the markers of memory B cells. We also show that these cells do not act as antigen presenting cells, as indicated by the low levels of CD80 and DR, nor do they express significant levels of the CD40 molecule necessary to interact with T lymphocytes through the ligand, CD154. Hence, we hypothesize that these expanded cells are late memory or exhausted cells that have down-modulated the expression of CD27 and filled the immunologic space in the elderly. These cells might be the age-related manifestation of time-enduring stimulation or dysregulation of the immune system.
- Published
- 2009
31. Double Negative (IgG+IgD-CD27-) B Cells are Increased in a Cohort of Moderate-Severe Alzheimer’s Disease Patients and Show a Pro-Inflammatory Trafficking Receptor Phenotype
- Author
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Matteo Bulati, Francesco Gervasi, Giuseppina Colonna-Romano, Calogero Caruso, Silvio Buffa, Delia Azzarello, Cecilia Camarda, Roberto Monastero, Adriana Martorana, Bulati, M, Buffa, S, Martorana, A, Gervasi, F, Camarda, C, Azzarello, D, Monastero, R, Caruso, C, and Colonna-Romano G
- Subjects
Male ,Receptors, CCR6 ,Receptors, CCR7 ,Myeloid ,Lymphocyte ,B-Lymphocyte Subsets ,C-C chemokine receptor type 7 ,Inflammation ,C-C chemokine receptor type 6 ,Immunoglobulin D ,CD19 ,Cohort Studies ,Alzheimer Disease ,medicine ,Humans ,B cell ,Aged ,Aged, 80 and over ,Settore MED/04 - Patologia Generale ,biology ,General Neuroscience ,General Medicine ,Flow Cytometry ,Tumor Necrosis Factor Receptor Superfamily, Member 7 ,Psychiatry and Mental health ,Clinical Psychology ,medicine.anatomical_structure ,Phenotype ,Alzheimer's Disease, Inflammation, B Cells ,Immunoglobulin G ,Immunology ,biology.protein ,Female ,Settore MED/26 - Neurologia ,Geriatrics and Gerontology ,medicine.symptom ,Mental Status Schedule - Abstract
Alzheimer's disease (AD) is a progressive, irreversible, and debilitating disease for which no effective preventive or disease modifying therapies or treatments have so far been detected. The crucial step in AD pathogenesis is the production of amyloid-42 peptide, which causes chronic inflammation. Activated cells in the central nervous system (CNS) produce pro- inflammatory mediators that lead to the recruitment of myeloid or lymphocytic cells. As a consequence, the communication between the CNS and peripheral blood of AD subjects could influence the lymphocyte distribution and/or the expression of phenotypic markers. In the present paper, we show a significant decrease in total CD19 + B lymphocytes and a remodeling of the B cell subpopulations in moderate-severe AD patients, compared with their coeval healthy controls and mild AD subjects. In particular, we report a significant reduction in na¨ ove B cells (IgD + CD27 − ) and a simultaneous increase in double negative (DN, IgD − CD27 − ) memory B lymphocytes. We have also evaluated the expression of the pro-inflammatory chemokine receptors CCR6 and CCR7 in total and na¨ ove/memory B cells from mild and moderate-severe AD patients, with the aim to detect a possible relationship between the trafficking profile and the stage of the disease. Our results demonstrate that both the amount and the trafficking profile of B cells are related to the severity of AD. The results discussed in this paper suggest a well-selected antibody panel should be used as an additional test for the identification of early AD.
- Published
- 2015
32. Inflammation, Longevity, and Cardiovascular Diseases: Role of Polymorphisms of TLR4
- Author
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Giuseppina Candore, Giuseppina Colonna-Romano, Florinda Listì, Daniele Di Carlo, Domenico Lio, Carmela Rita Balistreri, Calogero Caruso, Valentina Orlando, Sonya Vasto, Marco Caruso, Alessandra Aquino, Matteo Bulati, Maria Paola Grimaldi, CANDORE G, AQUINO A, BALISTRERI CR, BULATI M, DI CARLO D, GRIMALDI MP, LISTI' F, ORLANDO V, VASTO S, CARUSO M, COLONNA-ROMANO G, LIO D, and CARUSO C
- Subjects
Adult ,Lipopolysaccharides ,Male ,Heterozygote ,Time Factors ,media_common.quotation_subject ,medicine.medical_treatment ,Longevity ,Myocardial Infarction ,Enzyme-Linked Immunosorbent Assay ,Inflammation ,Biology ,General Biochemistry, Genetics and Molecular Biology ,AMI ,History and Philosophy of Science ,medicine ,Humans ,Genetic Predisposition to Disease ,TLR4 ,Interleukin 6 ,media_common ,Polymorphism, Genetic ,Innate immune system ,Interleukin-6 ,General Neuroscience ,Interleukin ,Heterozygote advantage ,Middle Aged ,Toll-Like Receptor 4 ,Cytokine ,Acute Disease ,Mutation ,Immunology ,biology.protein ,Female ,medicine.symptom - Abstract
The total burden of infection at various sites may affect the progression of atherosclerosis, the risk being modulated by host genotype. The role of lipopolysaccaride receptor TLR4 is paradigmatic. It initiates the innate immune response against gram-negative bacteria; and TLR4 polymorphisms, as ASP299GLY, suggested to attenuate receptor signaling, have been described. We demonstrated that TLR4 ASP299GLY polymorphism shows a significantly lower frequency in patients affected by myocardial infarction compared to controls, whereas centenarians show a higher frequency. Thus, people genetically predisposed to developing weak inflammatory activity, seem to have fewer chances of developing cardiovascular diseases (CVD) and, subsequently, live longer if they do not become affected by serious infectious diseases. These results are in agreement with our other data demonstrating how genetic background may exert the opposite effect with respect to inflammatory components in CVD and longevity. In the present report, to validate this hypothesis, the levels of interleukin (IL)-6, a pro-inflammatory cytokine involved in atherosclerosis and longevity, were determined by an enzyme-linked immuno-sorbent assay (ELISA) in supernatants from a whole blood assay after stimulation with subliminal doses of lipopolysaccaride (LPS) from Escherichia coli (E. coli). The samples, genotyped for the ASP299GLY polymorphism, were challenged with LPS for 4, 24, and 48 h. What we found was that Il-6 values were significantly lower in carriers bearing TLR4 mutation. Therefore, the pathogen burden, by interacting with host genotype, determines the type and intensity of the immune-inflammatory responses accountable for pro-inflammatory status, CVD, and unsuccessful aging. On the other hand, our present data seem to explain the inconclusive results obtained in case-control studies taking into account the role of functional IL-6 polymorphisms in successful and unsuccessful aging. In fact, IL6 levels seem to depend, in addition, on IL-6 polymorphisms and on innate immunity gene polymorphisms as well.
- Published
- 2006
33. Trafficking phenotype and production of granzyme B by double negative B cells (IgG(+)IgD(-)CD27(-)) in the elderly
- Author
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Calogero Caruso, Giuseppina Colonna-Romano, Matteo Bulati, Adriana Martorana, Domenico Lio, Silvio Buffa, Giuseppina Candore, Bulati M, Buffa S, Martorana A, Candore G, Lio D, Caruso C, and Colonna-Romano G
- Subjects
Adult ,Aging ,Chemokine receptor ,Naive B cell ,B-cell receptor ,B-Lymphocyte Subsets ,chemical and pharmacologic phenomena ,Biology ,CXCR3 ,Biochemistry ,Granzymes ,Endocrinology ,Immune system ,Elderly ,IL-21 ,Genetics ,Humans ,Settore MED/05 - Patologia Clinica ,L-Selectin ,Memory B cell ,Molecular Biology ,Aged ,Aged, 80 and over ,Receptors, CXCR ,Settore MED/04 - Patologia Generale ,B lymphocyte ,Granzyme B ,Interleukins ,hemic and immune systems ,Immunoglobulin D ,Cell Biology ,Inflamm-aging ,Tumor Necrosis Factor Receptor Superfamily, Member 7 ,B-1 cell ,Immunosurveillance ,Phenotype ,Immunoglobulin G ,Immunology - Abstract
The impairment of humoral immune response in elderly humans has been extensively demonstrated. We have reported the increase of memory B cells (IgG(+)IgD(-)CD27(-), double negative, DN) population in the elderly, in which there is also a typical inflammatory micro-environment. In order to evaluate whether this pro-inflammatory status could influence the trafficking phenotype of naïve/memory B cells, we have assessed the expression of CCR7, CCR6, CXCR3, CXCR4, CXCR5 and CD62L on naïve/memory B cell subpopulations in young and elderly subjects. Moreover, the combination of pro-inflammatory interleukin-21 (IL-21) and B cell receptor (BCR) stimulation enables B cells to produce and secrete granzyme B (GrB), which plays a critical role in early anti-viral immune responses, in the regulation of autoimmune mechanisms and in cancer immunosurveillance. Our data demonstrate that in the elderly, naïve/memory B cell populations present a different expression of the studied receptors that could be discussed in terms of "inflamm-aging". In particular IgG(+)IgD(-)CD27(-) DN B cells show a tissue trafficking phenotype and they can be stimulated to produce GrB.
- Published
- 2014
34. A novel B cell population revealed by a CD38/CD24 gating strategy: CD38−CD24− B cells in centenarian offspring and elderly people
- Author
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Mariavaleria Pellicanò, Graham Pawelec, Calogero Caruso, Giuseppina Colonna-Romano, David Goldeck, Adriana Martorana, Matteo Bulati, Silvio Buffa, Buffa, S, Pellicanò, M, Bulati, M, Martorana, A, Goldeck, D, Caruso, C, Pawelec, G, and Colonna-Romano, G
- Subjects
Adult ,Male ,Parents ,Aging ,CD180 ,Offspring ,Immunosenescence ,Population ,B cell ,CD38 ,CD24 ,Centenarian offspring ,Longevity ,Lymphocyte Activation ,CD19 ,Article ,03 medical and health sciences ,0302 clinical medicine ,Reference Values ,medicine ,Humans ,education ,030304 developmental biology ,Aged ,Settore MED/04 - Patologia Generale ,Aged, 80 and over ,0303 health sciences ,education.field_of_study ,B-Lymphocytes ,Immunity, Cellular ,biology ,CD24 Antigen ,General Medicine ,Middle Aged ,Acquired immune system ,ADP-ribosyl Cyclase 1 ,3. Good health ,medicine.anatomical_structure ,Immunology ,biology.protein ,Cytokines ,Female ,Geriatrics and Gerontology ,Centenarian ,030215 immunology - Abstract
The B cell arm of adaptive immunity undergoes significant modifications with age. Elderly people are characterized by impaired B cell responses reflected in a reduced ability to effectively respond against viruses and bacteria. Alterations of immunity with advancing age (immunosenescence) have been widely studied in centenarians who are considered a good example of successful aging. In recent years, attention has shifted to centenarian offspring (CO) as a model of people genetically advantaged for healthy aging and longevity. Here, we describe the preliminary characterization of a proposed new population of memory B cells, defined as CD19(+)CD38(-)CD24(-), which we find at higher frequencies in the elderly but less so in CO than healthy age-matched random controls. In addition, we found a decreased expression of RP105 (CD180), a toll-like receptor-associated molecule, on these cells. CD180 downregulation may potentially be a marker of immunosenescence. Moreover, we show that these CD19(+)CD38(-)CD24(-) B cells produce TNF and hypothesize that their observed expansion in the elderly might contribute to the increased inflammatory status sometimes designated "inflamm-aging."
- Published
- 2012
35. Genetics of longevity. Data from the studies on Sicilian centenarians
- Author
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Matteo Bulati, Marisa Palmeri, Silvio Buffa, M Bova, Loredana Vaccarino, Domenico Lio, Giuseppina Colonna-Romano, Mariavaleria Pellicanò, Giusi Irma Forte, Carmela Rita Balistreri, Giulia Accardi, Giuseppina Candore, Florinda Listì, Letizia Scola, Adriana Martorana, Balistreri, CR, Candore, G, Accardi, G, Bova, V, Buffa, S, Bulati, M, Forte, GI, Listì, F, Martorana, A, Palmeri, M, Pellicanò, M, Vaccarino, L, Scola, L, Lio, D, and Colonna-Romano G
- Subjects
lcsh:Immunologic diseases. Allergy ,Epigenomics ,Gerontology ,Aging ,medicine.medical_specialty ,Future studies ,Immune system, Genetics, Pro/anti-inflammatory polymorphisms, Epigenomics ,media_common.quotation_subject ,Immunology ,lcsh:Geriatrics ,Biology ,Genetics ,medicine ,Settore MED/05 - Patologia Clinica ,Epigenetics ,Inflammatory genes ,media_common ,Research ,Public health ,Longevity ,Ageing ,lcsh:RC952-954.6 ,Immune system ,Pro/anti-inflammatory polymorphisms ,Life expectancy ,lcsh:RC581-607 - Abstract
The demographic and social changes of the past decades have determined improvements in public health and longevity. So, the number of centenarians is increasing as a worldwide phenomenon. Scientists have focused their attention on centenarians as optimal model to address the biological mechanisms of "successful and unsuccessful ageing". They are equipped to reach the extreme limits of human life span and, most importantly, to show relatively good health, being able to perform their routine daily life and to escape fatal age-related diseases, such as cardiovascular diseases and cancer. Thus, particular attention has been centered on their genetic background and immune system. In this review, we report our data gathered for over 10 years in Sicilian centenarians. Based on results obtained, we suggest longevity as the result of an optimal performance of immune system and an over-expression of anti-inflammatory sequence variants of immune/inflammatory genes. However, as well known, genetic, epigenetic, stochastic and environmental factors seem to have a crucial role in ageing and longevity. Epigenetics is associated with ageing, as demonstrated in many studies. In particular, ageing is associated with a global loss of methylation state. Thus, the aim of future studies will be to analyze the weight of epigenetic changes in ageing and longevity.
- Published
- 2012
36. TRafficking profile in naive and memory B cells in young and old subjects
- Author
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BULATI, Matteo, BUFFA, Silvio, MARTORANA, Adriana, BALISTRERI, Carmela Rita, PELLICANO', Mariavaleria, CANDORE, Giuseppina, LIO, Domenico, CARUSO, Calogero, COLONNA ROMANO, Giuseppina, Bulati, M, Buffa, S, Martorana, A, Balistreri, CR, Pellicanò, M, Candore,G, Lio, D, Caruso,C, and Colonna-Romano G
- Subjects
memory B cells, ageing, chemokine receptors ,Settore MED/05 - Patologia Clinica - Published
- 2012
37. Vascular Aging effect on Medial aorta degeneration: focus on blood keukocyte telomere lenght in Hypertensive and old patients with sporadic thoracic aortic aneurysm
- Author
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BALISTRERI, Carmela Rita, PISANO, Calogera, MARTORANA, Adriana, BULATI, Matteo, BUFFA, Silvio, CANDORE, Giuseppina, COLONNA ROMANO, Giuseppina, RUVOLO, Giovanni, LIO, Domenico, CARUSO, Calogero, Balistreri, CR, Pisano, C, Martorana, A, Bulati, M, Buffa, S, Candore, G, Colonna-Romano, G, Ruvolo, G, Lio, D, and Caruso, C
- Subjects
vascular ageing, sporadic thoracic aortic aneurysm, blood leukocyte telomere lenght ,Settore MED/05 - Patologia Clinica - Published
- 2012
38. Immunosenescence, inflammation and Alzheimer’s disease
- Author
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Matteo Bulati, Mariavaleria Pellicanò, Calogero Caruso, Giuseppina Colonna-Romano, Silvio Buffa, Giuseppina Candore, Adriana Martorana, Martorana, A, Bulati, M, Buffa, S, Pellicanò, M, Caruso, C, Candore, G, and Colonna-Romano, G
- Subjects
Immunosenescence ,Alzheimer’s disease ,Inflammation ,Cytokine ,Chemokine ,Lymphocyte ,Ageing ,Review ,Disease ,Immune system ,medicine ,Dementia ,Settore MED/04 - Patologia Generale ,Innate immune system ,business.industry ,biochemical phenomena, metabolism, and nutrition ,medicine.disease ,Acquired immune system ,Immunology ,bacteria ,sense organs ,medicine.symptom ,business - Abstract
Ageing impacts negatively on the development of the immune system and its ability to fight pathogens. Progressive changes in the T-cell and B-cell systems over the lifespan of individuals have a major impact on the capacity to respond to immune challenges. The cumulative age-associated changes in immune competence are termed immunosenescence that is characterized by changes where adaptive immunity deteriorates, while innate immunity is largely conserved or even upregulated with age. On the other hand, ageing is also characterized by “inflamm-ageing”, a term coined to explain the inflammation commonly present in many age-associated diseases. It is believed that immune inflammatory processes are relevant in Alzheimer’s disease, the most common cause of dementia in older people. In the present paper we review data focusing on changes of some immunoinflammatory parameters observed in patients affected by Alzheimer’s disease.
- Published
- 2012
39. B cell immunosenescence: different features of naive and memory B cells in elderly
- Author
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Matteo Bulati, Silvio Buffa, Giuseppina Candore, Salvatore Vitello, Mariavaleria Pellicanò, Deborah K. Dunn-Walters, Calogero Caruso, Yu-Chang Wu, Giuseppina Colonna-Romano, Buffa, S, Bulati, M, Pellicanò, M, Dunn-Walters, DK, Wu, YC, Candore, G, Vitello, S, Caruso, C, and Colonna-Romano, G
- Subjects
Adult ,Aging ,Naive B cell ,Somatic hypermutation ,Immunoglobulins ,Inflammation ,Biology ,Lymphocyte Activation ,Elderly ,medicine ,Humans ,Cytokine ,B cell ,Cellular Senescence ,Aged ,Settore MED/04 - Patologia Generale ,Aged, 80 and over ,B-Lymphocytes ,Hypermutation ,Ionomycin ,Germinal center ,Immunosenescence ,Middle Aged ,Memory B cells ,Interleukin-10 ,B-1 cell ,Interleukin 10 ,medicine.anatomical_structure ,Immunology ,Tetradecanoylphorbol Acetate ,Geriatrics and Gerontology ,Gerontology ,Cell aging ,Immunologic Memory - Abstract
Elderly people show a reduced protection against new infections and a decreased response to vaccines as a consequence of impairment of both cellular and humoral immunity. In this paper we have studied memory/naive B cells in the elderly, evaluating surface immunoglobulin expression, production of the pro- and anti-inflammatory cytokines, tumor necrosis factor (TNF)-α and interleukin (IL)-10, and presence of somatic hypermutation, focusing on the IgG(+)IgD(-)CD27(-) double negative (DN) B cells that are expanded in the elderly. Our results show that naive B cells from young donors need a sufficiently strong stimulus to be activated "in vitro", while naive B cells from old subjects are able to produce IL-10 and TNF-α when stimulated "physiologically" (α-CD40/IL-4), suggesting that these cells might play a role in the control of the immuno-inflammatory environment in the elderly. In addition, in the elderly there is an accumulation of DN B cells with a reduced rate of somatic hypermutation. Thus, DN B lymphocytes may be exhausted cells that are expanded and accumulate as a by-product of persistent stimulation or impaired germinal center formation.
- Published
- 2011
40. Studio longitudinale degli effetti della psicoterapia sulla funzionalità immunitaria in soggetti affetti da disturbo depressivo unipolare
- Author
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VALSAVOIA, Rosaria, BUFFA, Silvio, BULATI, Matteo, BRUNO, Alessandro, DI CARO, Angela, FERRARO, Laura, FUCA', Laura, LA CASCIA, Caterina, MULÈ, Alice, PELLICANO', Mariavaleria, VIRGILIO, Pietro, COLONNA ROMANO, Giuseppina, LA BARBERA, Daniele, Marchese, F, Messina, N, Trotta, A, Valsavoia, R, Buffa, S, Bulati, M, Bruno, A, Di Caro, A, Ferraro, L, Fucà, L, La Cascia, C, Marchese, F, Messina, N, Mulè, A, Pellicanò, M, Trotta, A, Virgilio, P, Colonna Romano, G, La Barbera, D, and La Barbera D
- Subjects
Settore MED/04 - Patologia Generale ,depressione, immunità, psicoterapia ,Settore MED/25 - Psichiatria ,depressive disorders, immunology - Published
- 2010
41. Inflammation, Cytokines, Immune Response, Apolipoprotein E, Cholesterol, and Oxidative Stress in Alzheimer Disease: Therapeutic Implications
- Author
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Sonya Vasto, Danilo Di Bona, Laura Castiglia, Giovanni Scapagnini, Claudia Rizzo, Calogero Caruso, Mariavaleria Pellicanò, Matteo Bulati, Domenica Matranga, Giovanni Duro, Domenico Lio, Giuseppina Candore, Giuseppina Colonna-Romano, Candore,G, Bulati,M, Caruso,C, Castiglia,L, Colonna-Romano,G, Di Bona,D, Duro,G, Lio,D, Matranga,D, Pellicanò, M, Rizzo, CM, Scapagnini,G, and Vasto,S
- Subjects
Apolipoprotein E ,Aging ,Alzheimer Disease, Inflammation ,medicine.medical_treatment ,Inflammation ,Disease ,medicine.disease_cause ,Immune System Phenomena ,Immune system ,Apolipoproteins E ,Alzheimer Disease ,medicine ,Animals ,Humans ,Settore MED/04 - Patologia Generale ,business.industry ,Immunotherapy ,medicine.disease ,Diet ,Oxidative Stress ,Cytokine ,Cholesterol ,Immunology ,Cytokines ,Geriatrics and Gerontology ,medicine.symptom ,Alzheimer's disease ,business ,Oxidative stress - Abstract
Alzheimer disease (AD) is a heterogeneous and progressive neurodegenerative disease, which in Western society mainly accounts for senile dementia. Today many countries have rising aging populations and are facing an increased prevalence of age-related diseases, such as AD, with increasing health-care costs. Understanding the pathophysiology process of AD plays a prominent role in new strategies for extending the health of the elderly population. Considering the future epidemic of AD, prevention and treatment are important goals of ongoing research. However, a better understanding of AD pathophysiology must be accomplished to make this objective feasible. In this paper, we review some hot topics concerning AD pathophysiology that have an important impact on therapeutic perspectives. Hence, we have focused our attention on inflammation, cytokines, immune response, apolipoprotein E (APOE), cholesterol, oxidative stress, as welll as exploring the related therapeutic possibilities, i.e., nonsteroidal antiinflammatory drugs, cytokine blocking antibodies, immunotherapy, diet, and curcumin.
- Published
- 2010
42. Sottopopolazioni linfocitarie in soggetti affetti da disturbo depressivo unipolare
- Author
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VALSAVOIA, Rosaria, BULATI, Matteo, PELLICANO', Mariavaleria, COLONNA ROMANO, Giuseppina, LA BARBERA, Daniele, Valsavoia, R, Bulati, M, Pellicanò, M, Colonna Romano, G, and La Barbera, D
- Subjects
Settore MED/25 - Psichiatria ,depressive disorders, immunology - Published
- 2010
43. Biomarkers of aging
- Author
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VASTO, Sonya, BULATI, Matteo, CANDORE, Giuseppina, CASTIGLIA, Laura, COLONNA ROMANO, Giuseppina, LIO, Domenico, PELLICANO', Mariavaleria, RIZZO, Claudia Maria, CARUSO, Calogero, Scapagnini, G, Nuzzo, D, Ferrara, N, Vasto, S, Scapagnini, G, Bulati, M, Candore, G, Castiglia, L, Colonna-Romano, G, Lio, D, Nuzzo, D, Pellicano', M, Rizzo, CM, Ferrara, N, and Caruso, C
- Subjects
Settore MED/04 - Patologia Generale ,Settore MED/05 - Patologia Clinica ,Immunosenescence, Aging, Inflammation, biomarkers - Abstract
Aging is a complex process that negatively impacts the development of the different systems and its ability to function. Moreover, the Aging rate in humans is not the same, principally due to genetic heterogeneity and environmental factors. The aging rate is measured as the decline of functional capacity and stress resistance. Therefore, several attempts have been made to analyse the individual age, ( so-called biological age) compared to chronological age. The biomarkers of aging are age-related body function or composition, these markers aim to assess the biological age and predict the onset of age-related diseases and/or residual lifetime. Such biomarkers should help in one hand to characterise the biological age and on the other hand to identify individuals at high risk of developing age-associated diseases or disabilities. Unfortunately, most of the markers under discussion are related to age-related diseases rather than to age, so none of these markers discussed in literature is a true biomarker of aging. Hence, we discuss some disease-related biomarkers useful for a better understanding of aging and the development of new strategies to counteract it, essential for improving the quality of life of the elderly population.
- Published
- 2010
44. Immune-inflammatory responses in successful and unsuccessful ageing
- Author
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CANDORE, Giuseppina, BALISTRERI, Carmela Rita, BULATI, Matteo, COLONNA ROMANO, Giuseppina, FORTE, Giusi Irma, LIO, Domenico, LISTI', Florinda, PELLICANO', Mariavaleria, SCOLA, Letizia, VASTO, Sonya, CARUSO, Calogero, Di Bona, D, Candore, G, Balistreri, CR, Bulati, M, Colonna-Romano, G, Di Bona, D, Forte, GI, Lio, D, Listì, F, Pellicanò, M, Scola, L, Vasto, S, and Caruso, C
- Subjects
Inflammation ,Settore MED/04 - Patologia Generale ,Ageing ,Immunogenetics - Abstract
A dramatic increase in mean life span and life expectancy, coupled with a significant reduction in early mortality, has lead to a large increase in number of elderly people in modern societies. This demographic phenomenon has been paralleled by an epidemic of chronic diseases associated with advancing age. Both innate and instructive immunity are implicated in almost all age-related diseases. The modifications of the immune system in the elderly are evaluated as a deterioration of the immune system, the so-called immunosenescence, which is thought to be mostly the result of the declining effectiveness of T cells and it is responsible for the increased susceptibility of elderly to infectious diseases. In addition, a low-grade systemic inflammation characterizes ageing and inflammatory markers are significant predictors of mortality in old humans. This pro-inflammatory status of the elderly underlies biological mechanisms responsible for physical function decline and inflammatory age-related diseases are initiated or worsened by systemic inflammation.
- Published
- 2009
45. B cell immunosenescence in the elderly and in centenarians
- Author
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Giuseppina Candore, Domenico Lio, Giuseppina Colonna-Romano, Salvatore Vitello, Calogero Caruso, Alessandra Aquino, Matteo Bulati, Colonna Romano, G, Bulati, M, Aquino, A, Vitello, S, Lio, D, Candore, G, and Caruso, C
- Subjects
Settore MED/04 - Patologia Generale ,Aged, 80 and over ,Aging ,B-Lymphocytes ,Lymphocyte ,T cell ,Naive B cell ,Immunosenescence ,Biology ,medicine.anatomical_structure ,Immune system ,Antigen ,Immunology ,medicine ,Humans ,elderly, chronic antigen exposure, senescence of B cells ,Geriatrics and Gerontology ,Cell aging ,B cell ,Cellular Senescence ,Aged - Abstract
The elderly suffer from an increased susceptibility to infectious disease and cancer. Aging of the immune system contributes to this state of affairs due to immunosenescence. Because repeated intermittent or chronic antigen exposure may lead to lymphocyte clonal exhaustion, chronic antigenic stress plays a part in the compromised immunity of the elderly, who have accumulated a lifetime's exposure to infectious agents, autoantigens, and cancer antigens. Literature on immunosenescence has focused mainly on T cell impairment, but B cell compartment is also affected. The age-dependent B cell changes documented by the present review indicate that advanced age per se is a condition characterized by lack of B clonotypic immune response to new extracellular pathogens. In any event, data are suggesting that the loss of naive B cells could represent a hallmark of immunosenescence and could provide a biomarker possibly related to the life span of humans and potentially useful for the evaluation of anti-aging treatment. Since information on the senescence of B cells is of obvious interest, further studies are necessary to confirm these suggestions as well as to extend the number of markers used to characterize the cells.
- Published
- 2008
46. Understanding ageing: Biomedical and bioengineering approaches, the immunologic view
- Author
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Mariavaleria Pellicanò, Giuseppina Colonna-Romano, Sonya Vasto, Matteo Bulati, Bulati, M, Pellicano', M, Vasto, S, and Colonna Romano, G
- Subjects
Gerontology ,lcsh:Immunologic diseases. Allergy ,Settore MED/04 - Patologia Generale ,Aging ,business.industry ,Mitochondrial damage, immunology, Telomeres ,Immunology ,Short Report ,Immunosenescence ,lcsh:Geriatrics ,Birth rate ,Sharp rise ,Ageing ,lcsh:RC952-954.6 ,Elderly population ,Life expectancy ,Medicine ,business ,Older people ,lcsh:RC581-607 ,Developed country - Abstract
During the past century, humans have gained more years of average life expectancy than in the last 10,000 years; we are now living in a rapidly ageing world. The sharp rise in life expectancy, coupled to a steady decline in birth rates in all developed countries, has led to an unprecedented demographic revolution characterized by an explosive growth in the number and proportion of older people. Ageing is a complex process that negatively impacts the development of the immune system and its ability to function. Progressive changes in the T and B cell systems over the life span have a major impact on the capacity to respond to immune challenge. These cumulative age-associated changes in immune competence are termed Immunosenescence: some immunological parameters are commonly notably reduced in the elderly and, reciprocally good function is tightly correlated to health status. Hence, a better understanding of Immunosenescence and the development of new strategies to counteract it are essential for improving the quality of life of the elderly population.
- Published
- 2008
47. Impact of CMV and EBV seropositivity on CD8 T lymphocytes in an old population from West-Sicily
- Author
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Jean M. Fletcher, Graham Pawelec, Matteo Bulati, Pietro Ammatuna, Giuseppina Candore, Domenico Lio, Giuseppina Colonna-Romano, Calogero Caruso, Arne N. Akbar, Alessandra Aquino, Colonna Romano, G, Arne Akbar, N, Aquino, A, Bulati, M, Candore, G, Lio, D, Ammatuna, P, Fletcher, J, Caruso, C, and Pawelec, G
- Subjects
Human cytomegalovirus ,Adult ,Male ,Aging ,Epstein-Barr Virus Infections ,Herpesvirus 4, Human ,Population ,Cytomegalovirus ,Epitopes, T-Lymphocyte ,Biology ,CD8-Positive T-Lymphocytes ,Antibodies, Viral ,Biochemistry ,Epitope ,Virus ,Immunophenotyping ,Elderly ,Endocrinology ,Immune system ,EBV ,T-Lymphocyte Subsets ,HLA-A2 Antigen ,Genetics ,medicine ,Cytotoxic T cell ,Humans ,education ,Molecular Biology ,Sicily ,Aged ,Settore MED/04 - Patologia Generale ,Aged, 80 and over ,education.field_of_study ,CMV ,CD8 ,Immune senescence ,Cell Biology ,Immunosenescence ,Middle Aged ,medicine.disease ,Virology ,Immunology ,Cytomegalovirus Infections ,Female - Abstract
Herpes viruses (particularly CMV and to some extent EBV) might play a role in accelerating the deterioration of immune functions with age. Indeed, it has been demonstrated that chronic infection with CMV causes an expansion of specific CD8 T lymphocytes and that this is related to a shrinkage of the T cell repertoire in very elderly people, predicting mortality. We have analysed CD8 T cells in young and old healthy Sicilians who were both CMV- and EBV-seropositive. Our data confirm expansions of T cells specific for the HLA-A2-restricted pp65 (495–503) CMV epitope up to nearly 14% of total peripheral CD8 cells in certain elderly individuals (range 0–14%). However, the mean percentage of CMV-specific cells in the elderly was not greater than the young (range 0.2–3%). The CMV-specific CD8 cells in the elderly were predominantly CD45RA+, but in the young they were mostly CD45RO+. Our findings are somewhat different from published reports from Northern European populations, both in terms of mean numbers and surface phenotypes. These findings may reflect disparate hygienic and nutritional conditions 70–90 yr ago, which were very different in Northern and Southern Europe at that time, as well as a different genetic background.
- Published
- 2007
48. Gruppi sanguigni e Sistema HLA
- Author
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COLONNA ROMANO, Giuseppina, BULATI, Matteo, CARUSO C, LICASTRO F., COLONNA-ROMANO G, and BULATI M
- Published
- 2007
49. B CELL IMMUNOSENESCENCE IN THE ELDERLY
- Author
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COLONNA ROMANO G, VITELLO S, CARUSO C., BULATI, Matteo, AQUINO, Alessandra, LIO, Domenico, CANDORE, Giuseppina, COLONNA-ROMANO G, BULATI M, AQUINO A, VITELLO S, LIO D, CANDORE G, and CARUSO C
- Published
- 2007
50. Linfociti
- Author
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COLONNA ROMANO, Giuseppina, BULATI, Matteo, CARUSO C, LICASTRO F., COLONNA-ROMANO G, and BULATI M
- Published
- 2007
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