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Your search keyword '"Buikema, H. J."' showing total 6 results
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6 results on '"Buikema, H. J."'

1. Digital image analysis of intraepithelial B-lymphocytes as an objective alternative to assess lymphoepithelial lesions in salivary glands of Sjögren's syndrome patients

Author

Van Ginkel, M. S., Van der Sluis, T., Bulthuis, M. L. C., Buikema, H. J., Haacke, E. A., Arends, S., Harder, S., Spijkervet, F. K. L., Bootsma, H., Vissink, A., Kroese, F. G. M., Van der Vegt, B., Translational Immunology Groningen (TRIGR), Guided Treatment in Optimal Selected Cancer Patients (GUTS), Personalized Healthcare Technology (PHT), and Damage and Repair in Cancer Development and Cancer Treatment (DARE)

Published
2022

2. POS0454 DIGITAL IMAGE ANALYSIS OF INTRAEPITHELIAL B-LYMPHOCYTES AS AN OBJECTIVE ALTERNATIVE TO ASSESS LYMPHOEPITHELIAL LESIONS IN SALIVARY GLANDS OF SJÖGREN’S SYNDROME PATIENTS

Author

Van Ginkel, M. S., primary, Van der Sluis, T., additional, Bulthuis, M. L. C., additional, Buikema, H. J., additional, Haacke, E. A., additional, Arends, S., additional, Harder, S., additional, Spijkervet, F. K. L., additional, Bootsma, H., additional, Vissink, A., additional, Kroese, F. G. M., additional, and Van der Vegt, B., additional

Published
2022
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4. Detecting cervical cancer by quantitative promoter hypermethylation assay on cervical scrapings: A feasibility study

Author

Reesink-Peters, N., Wisman, G. B. A., Jéronimo, C., Tokumaru, C. Y., Cohen, Y., Dong, S. M., Klip, H. G., Buikema, H. J., Albert Suurmeijer, Hollema, H., Boezen, H. M., Sidransky, D., Zee, A. G. J., Targeted Gynaecologic Oncology (TARGON), Guided Treatment in Optimal Selected Cancer Patients (GUTS), Life Course Epidemiology (LCE), and Groningen Research Institute for Asthma and COPD (GRIAC)

Subjects
SMEAR, AGE, CARCINOMA, TISSUE, MULTIPLE GENES, TUMOR-SUPPRESSOR, NEOPLASIA, DNA METHYLATION, DISEASE, SERUM
Abstract

Current morphology-based cervical cancer screening is associated with significant false-positive and false-negative results. Tumor suppressor gene hypermethylation is frequently present in cervical cancer. It is unknown whether a cervical scraping reflects the methylation status of the underlying epithelium, and it is therefore unclear whether quantitative hypermethylation specific PCR (QMSP) on cervical scrapings could be used as a future screening method augmenting the current approach. Cervical scrapings and paired fresh frozen cervical tissue samples were obtained from 53 cervical cancer patients and 45 controls. All scrapings were morphologically scored and analyzed with QMSP for the genes APC, DAPK, MGMT, and GSTP1. To adjust for DNA input, hypermethylation ratios were calculated against DNA levels of a reference gene. Hypermethylation ratios of paired fresh frozen tissue samples and scrapings of cervical cancer patients and controls were strongly related (Spearman correlation coefficient, 0.80 for APC, 0.98 for DAPK, and 0.83 for MGMT; P

Published
2004

6. Detection of cervical neoplasia by DNA methylation analysis in cervico-vaginal lavages, a feasibility study.

Author

Eijsink JJ, Yang N, Lendvai A, Klip HG, Volders HH, Buikema HJ, van Hemel BM, Voll M, Coelingh Bennink HJ, Schuuring E, Wisman GB, and van der Zee AG

Subjects
Feasibility Studies, Female, Humans, Neoplasm Staging, Pilot Projects, Self-Examination, Therapeutic Irrigation methods, Vagina pathology, Vaginal Smears, DNA Methylation, Uterine Cervical Neoplasms genetics, Uterine Cervical Neoplasms pathology, Uterine Cervical Dysplasia genetics, Uterine Cervical Dysplasia pathology
Abstract

Objective: To explore the feasibility of DNA methylation analysis for the detection of cervical neoplasia in self-obtained cervico-vaginal lavages., Methods: Lavages collected by a self-sampling device and paired cervical scrapings were obtained from 20 cervical cancer patients and 23 patients referred with an abnormal cervical smear (15 with high-grade cervical intraepithelial neoplasia (CIN2+) and 8 without CIN). All lavages and scrapings were analyzed by liquid based cytology (LBC), Hybrid Capture II (HC-II) for hr-HPV DNA detection and by DNA methylation analysis (JAM3, TERT, EPB41L3 and C13ORF18). Concordance between lavages and scrapings was measured by Cohen's Kappa (k)., Results: In lavages and scrapings from cervical cancer patients (n=20), methylation analysis was positive in 19 (95%) and 19 (95%), HC-II in 16 (80%) and 15 (75%) and LBC in 15 (75%) and 19 (95%), respectively. In lavages and scrapings from CIN2+ patients (n=15), methylation analysis was positive in 10 (67%) and 12 (80%), HC-II in 15 (100%) and 15 (100%) and LBC in 11 (73%) and 12 (80%), respectively. Concordance between cervical scrapings and lavages (n=43) was for LBC k=0.522 (p<0.001), hr-HPV testing k=0.551 (p<0.001) and DNA methylation analysis k=0.653 (p<0.001)., Conclusions: DNA methylation analysis in cervico-vaginal lavages obtained by a self-sampling device is feasible and its diagnostic performance appears to be at least comparable to the detection of cervical neoplasia by cytomorphology and hr-HPV. Our pilot study suggests that detection of cervical neoplasia by DNA methylation analysis in cervico-vaginal lavages warrants exploration of its use in large prospective studies., (Copyright © 2010 Elsevier Inc. All rights reserved.)

Published
2011
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