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4. Gravidity and malaria trends interact to modify P. falciparum densities and detectability in pregnancy: a 3-year prospective multi-site observational study

Author

Matambisso, Glória, Brokhattingen, Nanna, Maculuve, Sónia, Cisteró, Pau, Mbeve, Henriques, Escoda, Anna, Miguel, Judice, Buetas, Elena, de Jong, Ianthe, Cuna, Boaventura, Melembe, Cardoso, Ndimande, Nelo, Porras, Gemma, Chen, Haily, Tetteh, Kevin K. A., Drakeley, Chris, Gamain, Benoit, Chitnis, Chetan, Chauhan, Virander, Quintó, Llorenç, Galatas, Beatriz, Macete, Eusébio, and Mayor, Alfredo

Published
2022
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5. The multispecies microbial cluster of Fusobacterium, Parvimonas, Bacteroides and Faecalibacterium as a precision biomarker for colorectal cancer diagnosis

Author

Ladra, Susana, Poza, Margarita, Conde-Pérez, Kelly, Aja-Macaya, Pablo, Buetas, Elena, Trigo-Tasende, Noelia, Nasser-Ali, Mohammed, Rumbo-Feal, Soraya, Nión Cabeza, Paula, Martin-De Arribas, E., Estévez, Lara, Otero Alén, Begoña, Noguera, José Francisco, Concha, Ángel, Pardiñas López, Simón, Carda-Diéguez, Miguel, Gómez-Randulfe Rodríguez, Martín Igor, Martinez-Lago, Nieves, Aparicio, Luis A., Bou, Germán, Mira, Alex, Vallejo, J.A., Ladra, Susana, Poza, Margarita, Conde-Pérez, Kelly, Aja-Macaya, Pablo, Buetas, Elena, Trigo-Tasende, Noelia, Nasser-Ali, Mohammed, Rumbo-Feal, Soraya, Nión Cabeza, Paula, Martin-De Arribas, E., Estévez, Lara, Otero Alén, Begoña, Noguera, José Francisco, Concha, Ángel, Pardiñas López, Simón, Carda-Diéguez, Miguel, Gómez-Randulfe Rodríguez, Martín Igor, Martinez-Lago, Nieves, Aparicio, Luis A., Bou, Germán, Mira, Alex, and Vallejo, J.A.

Abstract

[Abstract] The incidence of colorectal cancer (CRC) has increased worldwide, and early diagnosis is crucial to reduce mortality rates. Therefore, new noninvasive biomarkers for CRC are required. Recent studies have revealed an imbalance in the oral and gut microbiomes of patients with CRC, as well as impaired gut vascular barrier function. In the present study, the microbiomes of saliva, crevicular fluid, feces, and non-neoplastic and tumor intestinal tissue samples of 93 CRC patients and 30 healthy individuals without digestive disorders (non-CRC) were analyzed by 16S rRNA metabarcoding procedures. The data revealed that Parvimonas, Fusobacterium, and Bacteroides fragilis were significantly over-represented in stool samples of CRC patients, whereas Faecalibacterium and Blautia were significantly over-abundant in the non-CRC group. Moreover, the tumor samples were enriched in well-known periodontal anaerobes, including Fusobacterium, Parvimonas, Peptostreptococcus, Porphyromonas, and Prevotella. Co-occurrence patterns of these oral microorganisms were observed in the subgingival pocket and in the tumor tissues of CRC patients, where they also correlated with other gut microbes, such as Hungatella. This study provides new evidence that oral pathobionts, normally located in subgingival pockets, can migrate to the colon and probably aggregate with aerobic bacteria, forming synergistic consortia. Furthermore, we suggest that the group composed of Fusobacterium, Parvimonas, Bacteroides, and Faecalibacterium could be used to design an excellent noninvasive fecal test for the early diagnosis of CRC. The combination of these four genera would significantly improve the reliability of a discriminatory test with respect to others that use a single species as a unique CRC biomarker.

Published
2024

6. The multispecies microbial cluster of Fusobacterium, Parvimonas, Bacteroides and Faecalibacterium as a precision biomarker for colorectal cancer diagnosis

Author

Conde‐Pérez, Kelly, primary, Aja‐Macaya, Pablo, additional, Buetas, Elena, additional, Trigo‐Tasende, Noelia, additional, Nasser‐Ali, Mohammed, additional, Rumbo‐Feal, Soraya, additional, Nión, Paula, additional, Arribas, Elsa Martín‐De, additional, Estévez, Lara S., additional, Otero‐Alén, Begoña, additional, Noguera, José F., additional, Concha, Ángel, additional, Pardiñas‐López, Simón, additional, Carda‐Diéguez, Miguel, additional, Gómez‐Randulfe, Igor, additional, Martínez‐Lago, Nieves, additional, Ladra, Susana, additional, Aparicio, Luis M. A., additional, Bou, Germán, additional, Mira, Álex, additional, Vallejo, Juan A., additional, and Poza, Margarita, additional

Published
2024
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7. Parvimonas micra can translocate from the subgingival sulcus of the human oral cavity to colorectal adenocarcinoma

Author

Conde‐Pérez, Kelly, primary, Buetas, Elena, additional, Aja‐Macaya, Pablo, additional, Martin‐De Arribas, Elsa, additional, Iglesias‐Corrás, Iago, additional, Trigo‐Tasende, Noelia, additional, Nasser‐Ali, Mohammed, additional, Estévez, Lara S., additional, Rumbo‐Feal, Soraya, additional, Otero‐Alén, Begoña, additional, Noguera, Jose F., additional, Concha, Ángel, additional, Pardiñas‐López, Simón, additional, Carda‐Diéguez, Miguel, additional, Gómez‐Randulfe, Igor, additional, Martínez‐Lago, Nieves, additional, Ladra, Susana, additional, Aparicio, Luis A., additional, Bou, Germán, additional, Mira, Alex, additional, Vallejo, Juan A., additional, and Poza, Margarita, additional

Published
2023
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8. Parvimonas micra can translocate from the subgingival sulcus of the human oral cavity to colorectal adenocarcinoma.

Author

Conde‐Pérez, Kelly, Buetas, Elena, Aja‐Macaya, Pablo, Martin‐De Arribas, Elsa, Iglesias‐Corrás, Iago, Trigo‐Tasende, Noelia, Nasser‐Ali, Mohammed, Estévez, Lara S., Rumbo‐Feal, Soraya, Otero‐Alén, Begoña, Noguera, Jose F., Concha, Ángel, Pardiñas‐López, Simón, Carda‐Diéguez, Miguel, Gómez‐Randulfe, Igor, Martínez‐Lago, Nieves, Ladra, Susana, Aparicio, Luis A., Bou, Germán, and Mira, Alex

Abstract

Oral and intestinal samples from a cohort of 93 colorectal cancer (CRC) patients and 30 healthy controls (non‐CRC) were collected for microbiome analysis. Saliva (28 non‐CRC and 94 CRC), feces (30 non‐CRC and 97 CRC), subgingival fluid (20 CRC), and tumor tissue samples (20 CRC) were used for 16S metabarcoding and/or RNA sequencing (RNAseq) approaches. A differential analysis of the abundance, performed with the ANCOM‐BC package, adjusting the P‐values by the Holm‐Bonferroni method, revealed that Parvimonas was significantly over‐represented in feces from CRC patients (P‐value < 0.001) compared to healthy controls. A total of 11 Parvimonas micra isolates were obtained from the oral cavity and adenocarcinoma of CRC patients. Genome analysis identified a pair of isolates from the same patient that shared 99.2% identity, demonstrating that P. micra can translocate from the subgingival cavity to the gut. The data suggest that P. micra could migrate in a synergistic consortium with other periodontal bacteria. Metatranscriptomics confirmed that oral bacteria were more active in tumor than in non‐neoplastic tissues. We suggest that P. micra could be considered as a CRC biomarker detected in non‐invasive samples such as feces. [ABSTRACT FROM AUTHOR]

Published
2024
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10. Parvimonas micra can translocate from the subgingival sulcus of the human oral cavity to colorectal adenocarcinoma

Author

Ladra, Susana, Poza, Margarita, Conde-Pérez, Kelly, Buetas, Elena, Aja-Macaya, Pablo, Martin-De Arribas, E., Iglesias Corrás, Iago, Trigo-Tasende, Noelia, Nasser-Ali, Mohammed, Estévez, Lara, Rumbo-Feal, Soraya, Otero Alén, Begoña, Noguera, José Francisco, Concha, Ángel, Pardiñas López, Simón, Carda-Diéguez, Miguel, Gómez-Randulfe Rodríguez, Martín Igor, Martinez-Lago, Nieves, Aparicio, Luis A., Bou, Germán, Mira, Alex, Vallejo, J.A., Ladra, Susana, Poza, Margarita, Conde-Pérez, Kelly, Buetas, Elena, Aja-Macaya, Pablo, Martin-De Arribas, E., Iglesias Corrás, Iago, Trigo-Tasende, Noelia, Nasser-Ali, Mohammed, Estévez, Lara, Rumbo-Feal, Soraya, Otero Alén, Begoña, Noguera, José Francisco, Concha, Ángel, Pardiñas López, Simón, Carda-Diéguez, Miguel, Gómez-Randulfe Rodríguez, Martín Igor, Martinez-Lago, Nieves, Aparicio, Luis A., Bou, Germán, Mira, Alex, and Vallejo, J.A.

Abstract

[Abstract] Oral and intestinal samples from a cohort of 93 colorectal cancer (CRC) patients and 30 healthy controls (non-CRC) were collected for microbiome analysis. Saliva (28 non-CRC and 94 CRC), feces (30 non-CRC and 97 CRC), subgingival fluid (20 CRC), and tumor tissue samples (20 CRC) were used for 16S metabarcoding and/or RNA sequencing (RNAseq) approaches. A differential analysis of the abundance, performed with the ANCOM-BC package, adjusting the P-values by the Holm-Bonferroni method, revealed that Parvimonas was significantly over-represented in feces from CRC patients (P-value < 0.001) compared to healthy controls. A total of 11 Parvimonas micra isolates were obtained from the oral cavity and adenocarcinoma of CRC patients. Genome analysis identified a pair of isolates from the same patient that shared 99.2% identity, demonstrating that P. micra can translocate from the subgingival cavity to the gut. The data suggest that P. micra could migrate in a synergistic consortium with other periodontal bacteria. Metatranscriptomics confirmed that oral bacteria were more active in tumor than in non-neoplastic tissues. We suggest that P. micra could be considered as a CRC biomarker detected in non-invasive samples such as feces.

Published
2023

11. Evidence for translocation of oral Parvimonas micra from the subgingival sulcus of the human oral cavity to the colorectal adenocarcinoma

Author

Conde-Pérez, Kelly, primary, Buetas, Elena, additional, Aja-Macaya, Pablo, additional, Arribas, Elsa Martín-De, additional, Iglesias-Corrás, Iago, additional, Trigo-Tasende, Noelia, additional, Nasser-Ali, Mohammed, additional, Estévez, Lara S., additional, Rumbo-Feal, Soraya, additional, Otero-Alén, Begoña, additional, Noguera, José F., additional, Concha, Ángel, additional, Pardiñas-López, Simón, additional, Carda-Diéguez, Miguel, additional, Gómez-Randulfe, Igor, additional, Martínez-Lago, Nieves, additional, Ladra, Susana, additional, Aparicio, Luis M. A., additional, Bou, Germán, additional, Mira, Álex, additional, Vallejo, Juan A., additional, and Poza, Margarita, additional

Published
2022
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12. Additional file 4 of Gravidity and malaria trends interact to modify P. falciparum densities and detectability in pregnancy: a 3-year prospective multi-site observational study

Author

Matambisso, Glória, Brokhattingen, Nanna, Maculuve, Sónia, Cisteró, Pau, Mbeve, Henriques, Escoda, Anna, Miguel, Judice, Buetas, Elena, de Jong, Ianthe, Cuna, Boaventura, Melembe, Cardoso, Ndimande, Nelo, Porras, Gemma, Chen, Haily, Tetteh, Kevin K. A., Drakeley, Chris, Gamain, Benoit, Chitnis, Chetan, Chauhan, Virander, Quintó, Llorenç, Galatas, Beatriz, Macete, Eusébio, and Mayor, Alfredo

Abstract

Additional file 4. Supplementary results (P. falciparum parasite densities).

Published
2022
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13. Additional file 2 of Gravidity and malaria trends interact to modify P. falciparum densities and detectability in pregnancy: a 3-year prospective multi-site observational study

Author

Matambisso, Glória, Brokhattingen, Nanna, Maculuve, Sónia, Cisteró, Pau, Mbeve, Henriques, Escoda, Anna, Miguel, Judice, Buetas, Elena, de Jong, Ianthe, Cuna, Boaventura, Melembe, Cardoso, Ndimande, Nelo, Porras, Gemma, Chen, Haily, Tetteh, Kevin K. A., Drakeley, Chris, Gamain, Benoit, Chitnis, Chetan, Chauhan, Virander, Quintó, Llorenç, Galatas, Beatriz, Macete, Eusébio, and Mayor, Alfredo

Abstract

Additional file 2. Supplementary methods (Quantitative suspension array assay).

Published
2022
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14. Additional file 1 of Gravidity and malaria trends interact to modify P. falciparum densities and detectability in pregnancy: a 3-year prospective multi-site observational study

Author

Matambisso, Glória, Brokhattingen, Nanna, Maculuve, Sónia, Cisteró, Pau, Mbeve, Henriques, Escoda, Anna, Miguel, Judice, Buetas, Elena, de Jong, Ianthe, Cuna, Boaventura, Melembe, Cardoso, Ndimande, Nelo, Porras, Gemma, Chen, Haily, Tetteh, Kevin K. A., Drakeley, Chris, Gamain, Benoit, Chitnis, Chetan, Chauhan, Virander, Quintó, Llorenç, Galatas, Beatriz, Macete, Eusébio, and Mayor, Alfredo

Abstract

Additional file 1: Fig. S1. Map of Manhiça and Magude District in southern Mozambique where the study was conducted. Fig. S2: Performance of the qPCRs and quantitative suspension array assays along with the different experiments. Fig. S3. Study profile. Fig. S4. Genetic complexity of the infections by study area and period. Fig. S5. Antibody levels by studied factors.

Published
2022
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15. Additional file 3 of Gravidity and malaria trends interact to modify P. falciparum densities and detectability in pregnancy: a 3-year prospective multi-site observational study

Author

Matambisso, Glória, Brokhattingen, Nanna, Maculuve, Sónia, Cisteró, Pau, Mbeve, Henriques, Escoda, Anna, Miguel, Judice, Buetas, Elena, de Jong, Ianthe, Cuna, Boaventura, Melembe, Cardoso, Ndimande, Nelo, Porras, Gemma, Chen, Haily, Tetteh, Kevin K. A., Drakeley, Chris, Gamain, Benoit, Chitnis, Chetan, Chauhan, Virander, Quintó, Llorenç, Galatas, Beatriz, Macete, Eusébio, and Mayor, Alfredo

Abstract

Additional file 3. Table S1. Characteristics of study participants. Table S2. Interactions between centers and study variables on parasitological outcomes. Table S3. Interactions between gravidity and temporal trends on parasitological outcomes. Table S4. P. falciparum parasite rates, density and detectability between centers. Table S5. Annual changes in parasitological outcomes in pregnant women from Ilha Josina. Table S6. Annual changes in antibody levels by site and gravidity group.

Published
2022
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16. P. Falciparum Densities and Detectability in Pregnancy During Declining Malaria: A Three-Year Prospective Multi-Site Observational Study

Author

Matambisso, Glória, primary, Brokhattingen, Nanna, additional, Maculuve, Sónia, additional, Cisteró, Pau, additional, Mbeve, Henriques, additional, Escoda, Anna, additional, Miguel, Judice, additional, Buetas, Elena, additional, de Jong, Ianthe, additional, Cuna, Boaventura, additional, Melembe, Cardoso, additional, Ndimande, Nelo, additional, Porras, Gemma, additional, Chen, Haily, additional, Tetteh, Kevin K.A., additional, Drakeley, Chris, additional, Gamain, Benoit, additional, Chitnis, Chetan, additional, Chauhan, Virander, additional, Quintó, Llorenç, additional, Galatas, Beatriz, additional, Macete, Eusébio, additional, and Mayor, Alfredo, additional

Published
2022
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17. Susceptibility to Severe Acute Respiratory Syndrome Coronavirus 2 Infection Among Children and Adults: A Seroprevalence Study of Family Households in the Barcelona Metropolitan Region, Spain

Author

Brotons, Pedro, Launes, Cristian, Buetas, Elena, Fumado, Vicky, Henares, Desiree, de Sevilla, Mariona Fernandez, Redin, Alba, Fuente-Soro, Laura, Cuadras, Daniel, Mele, Maria, Jou, Cristina, Millat, Pere, Jordan, Iolanda, Garcia-Garcia, Juan Jose, Bassat, Quique, Muñoz-Almagro, Carmen, Adroher, Cristina, Alsina, Laia, Ajanovic, Sara, Andueza, Ainhoa, Arias, Sara, Balanza, Nuria, Baro, Barbara, Bonet-Carne, Elisenda, Claverol, Joana, Cubells, Marta, Fortuny, Claudia, Garcia-Miguel, Aleix, Gratacos, Eduard, Hernandez, Maria, Morales, Monica, Pérez-Soler, Felipe, Ríos, María, Pons, Gemma, Torrents, Francesc, Uribesalgo, Iris, Valls, Anna, and Varo, Rosauro

Subjects
Pediatrics, medicine.disease_cause, 01 natural sciences, 0302 clinical medicine, Seroepidemiologic Studies, antibody, Pandemic, Medicine, 030212 general & internal medicine, Prospective Studies, Child, Formació d’anticossos, Volunteer, Seroprevalència, COVID-19 (malaltia), Pandemias, Coronavirus, media_common, Convalescence, Formación de anticuerpos, Síndrome respiratòria aguda, Infectious Diseases, AcademicSubjects/MED00290, medicine.symptom, Microbiology (medical), Adult, medicine.medical_specialty, 616.9, media_common.quotation_subject, prevalence, Asymptomatic, Pandèmies, 03 medical and health sciences, children, Seroprevalencia, Major Article, Seroprevalence, Humans, 0101 mathematics, business.industry, SARS-CoV-2, Public health, 010102 general mathematics, COVID-19, household, Cross-Sectional Studies, El Niño, Síndrome respiratorio agudo, Spain, business
Abstract

Background Susceptibility of children and adults to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and persistence of antibody response to the virus after infection resolution remain poorly understood, despite their significant public health implications. Methods A prospective cross-sectional seroprevalence study with volunteer families that included at least 1 first-reported adult case positive by SARS-CoV-2 by polymerase chain reaction (PCR) and at least 1 child aged Results A total of 381 family households including 381 first-reported PCR-positive adult cases and 1084 contacts (672 children, 412 adults) were enrolled. SARS-CoV-2 seroprevalence rates were 17.6% (118 of 672) in children and 18.7% (77 of 335) in adult contacts (P = .64). Among first-reported cases, seropositivity rates varied from 84.0% in adults previously hospitalized and tested within 6 weeks since the first positive PCR result to 31.5% in those not hospitalized and tested after that lag time (P Conclusions Children appear to have similar probability as adults to become infected by SARS-CoV-2 in quarantined family households but remain largely asymptomatic. Adult antibody protection against SARS-CoV-2 seems to be weak beyond 6 weeks post-infection confirmation, especially in cases that have experienced mild disease.

Published
2020

18. Susceptibility to Severe Acute Respiratory Syndrome Coronavirus 2 Infection Among Children and Adults: A Seroprevalence Study of Family Households in the Barcelona Metropolitan Region, Spain.

Author

Brotons, Pedro, Launes, Cristian, Buetas, Elena, Fumado, Vicky, Henares, Desiree, Sevilla, Mariona Fernandez de, Redin, Alba, Fuente-Soro, Laura, Cuadras, Daniel, Mele, Maria, Jou, Cristina, Millat, Pere, Jordan, Iolanda, Garcia-Garcia, Juan Jose, Bassat, Quique, Muñoz-Almagro, Carmen, and Group, Kids Corona Study

Subjects
RESEARCH, SEROPREVALENCE, SARS-CoV-2, COVID-19, CONVALESCENCE, CROSS-sectional method, FAMILIES, PUBLIC health, ANTIBODY formation, DISEASE susceptibility, DESCRIPTIVE statistics, DISEASE prevalence, METROPOLITAN areas, POLYMERASE chain reaction, STAY-at-home orders, LONGITUDINAL method, CHILDREN
Abstract

Background Susceptibility of children and adults to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and persistence of antibody response to the virus after infection resolution remain poorly understood, despite their significant public health implications. Methods A prospective cross-sectional seroprevalence study with volunteer families that included at least 1 first-reported adult case positive by SARS-CoV-2 by polymerase chain reaction (PCR) and at least 1 child aged <15 years living in the same household under strict home confinement was conducted in the metropolitan Barcelona Health Region, Spain, during the pandemic period 28 April 2020–3 June 2020. All household members were tested at home using a rapid SARS-CoV-2 antibody assay with finger prick–obtained capillary blood. Results A total of 381 family households including 381 first-reported PCR-positive adult cases and 1084 contacts (672 children, 412 adults) were enrolled. SARS-CoV-2 seroprevalence rates were 17.6% (118 of 672) in children and 18.7% (77 of 335) in adult contacts (P  = .64). Among first-reported cases, seropositivity rates varied from 84.0% in adults previously hospitalized and tested within 6 weeks since the first positive PCR result to 31.5% in those not hospitalized and tested after that lag time (P  < .001). Nearly all (99.9%) positive children were asymptomatic or had mild symptoms. Conclusions Children appear to have similar probability as adults to become infected by SARS-CoV-2 in quarantined family households but remain largely asymptomatic. Adult antibody protection against SARS-CoV-2 seems to be weak beyond 6 weeks post-infection confirmation, especially in cases that have experienced mild disease. [ABSTRACT FROM AUTHOR]

Published
2021
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