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Additional file3. Supplementary figures for visual assessment of sheep rumen samples using different preservation methods. Fig. S2 8 mL vial containing the sheep rumen sample preserved with TNx2, GHx2 or EtOH. Fig. S3 2 mL vials containing TNx2, GHx2 or EtOH and sheep rumen sample after the “bead-beating” step. Fig. S4 2 mL vials containing sheep rumen samples preserved using TNx2, GHx2, EtOH and the GRC method after centrifugation. Fig. S5) Principal component analysis (PCA) of the log10 relative abundance matrix using the RB approach for all non-failed samples (> 100,000 reads) including positive control samples.